CsaV3_6G034010 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G034010
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionCalcium-binding EF-hand family protein
Locationchr6: 18813080 .. 18813933 (-)
RNA-Seq ExpressionCsaV3_6G034010
SyntenyCsaV3_6G034010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGCTCCACACACATATTATATATTTTGGTAATAAACTTTCTCGTCTGTGTTTACATTTCAATTCAAAACCAAAGAAACAAAAACACCATGCCTCCAATTAGCTCCAAAGACTTGCACAGGATCTTCAAGAAGCTAGACAAGAATTGTGATGGCCTCATTTGCCTTCAAGAGCTCAACTGGCTCTTGGATAGCATTGGCATCCAATTAACCATGGAGGAGTTGGAATCATTTCTAGAGAGACCAAGCCTTGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCCAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCAGGGTGTGTGCAAGATAATGATAATGATCAGGGTCAGGATGATATGGAGATTGTATATTTGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCCTGCGACGAGCTCGAAAACGTGTTGGTGAAGCTCGAGCTTTGGGATGCAAGTAGAAGCGATGTAGATTATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAGTTCTTCTTTTAGAGTTTCAGAAAAGACTAGAAAAAAATATCCCCTTTGTTATATTGTTGTTAGATAGAGATGGAGTTTGGTCCGTTTATTTACATGTACAATTTGAGAAGCTGGGAAGCTTCAAGAATAAAAATTTTGGTCATGGAGTTCATGTAATTATATTTTTGGTGCTCCAATAAAATAATGCATTTCCACTCTCCTTTAATTTATATAGGATCAATGACTATGGTCTTGTGTGTGTATATATCTATATATAATATGTGTGTGG

mRNA sequence

ATGCCTCCAATTAGCTCCAAAGACTTGCACAGGATCTTCAAGAAGCTAGACAAGAATTGTGATGGCCTCATTTGCCTTCAAGAGCTCAACTGGCTCTTGGATAGCATTGGCATCCAATTAACCATGGAGGAGTTGGAATCATTTCTAGAGAGACCAAGCCTTGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCCAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCAGGGTGTGTGCAAGATAATGATAATGATCAGGGTCAGGATGATATGGAGATTGTATATTTGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCCTGCGACGAGCTCGAAAACGTGTTGGTGAAGCTCGAGCTTTGGGATGCAAGTAGAAGCGATGTAGATTATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAG

Coding sequence (CDS)

ATGCCTCCAATTAGCTCCAAAGACTTGCACAGGATCTTCAAGAAGCTAGACAAGAATTGTGATGGCCTCATTTGCCTTCAAGAGCTCAACTGGCTCTTGGATAGCATTGGCATCCAATTAACCATGGAGGAGTTGGAATCATTTCTAGAGAGACCAAGCCTTGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCCAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCAGGGTGTGTGCAAGATAATGATAATGATCAGGGTCAGGATGATATGGAGATTGTATATTTGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCCTGCGACGAGCTCGAAAACGTGTTGGTGAAGCTCGAGCTTTGGGATGCAAGTAGAAGCGATGTAGATTATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAG

Protein sequence

MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFLFFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT*
Homology
BLAST of CsaV3_6G034010 vs. NCBI nr
Match: XP_004149438.1 (probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetical protein Csa_018262 [Cucumis sativus])

HSP 1 Score: 343.2 bits (879), Expect = 1.3e-90
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

BLAST of CsaV3_6G034010 vs. NCBI nr
Match: XP_008441608.1 (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 putative calcium-binding protein CML44 [Cucumis melo var. makuwa])

HSP 1 Score: 307.0 bits (785), Expect = 1.0e-79
Identity = 148/165 (89.70%), Postives = 156/165 (94.55%), Query Frame = 0

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           M PISSKDLHRIFKKLDKNCDGL+CLQEL WLLD IGIQLTMEELESFLE+PSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQD+     +DD EIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDH----EDDTEIVYMAFKVFDMNGDGFISCDELENV 120

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           LV+LELWDASRS+VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of CsaV3_6G034010 vs. NCBI nr
Match: XP_038885742.1 (probable calcium-binding protein CML44 [Benincasa hispida])

HSP 1 Score: 280.0 bits (715), Expect = 1.3e-71
Identity = 137/165 (83.03%), Postives = 149/165 (90.30%), Query Frame = 0

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           M  IS  DLHRIF KLDKN DGLICLQEL WL D IGIQLTMEELESFLE+PSLDF+EFL
Sbjct: 11  MSSISFNDLHRIFNKLDKNGDGLICLQELKWLFDRIGIQLTMEELESFLEKPSLDFNEFL 70

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGV GC+QD+     +++MEIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 71  FFYESISKQNKGECKGGV-GCLQDDH----EEEMEIVYMAFKVFDMNGDGFISCDELENV 130

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           L +LELWDA+R+D+DYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 131 LSRLELWDANRNDIDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 170

BLAST of CsaV3_6G034010 vs. NCBI nr
Match: XP_022993348.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 258.8 bits (660), Expect = 3.1e-65
Identity = 128/163 (78.53%), Postives = 143/163 (87.73%), Query Frame = 0

Query: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFLF 61
           PPISS DL RIF +LDKN DGLIC+QEL WLLD+IGIQLTMEELESFLE+PSLDFDEFL 
Sbjct: 3   PPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLI 62

Query: 62  FYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENVL 121
           FYES+SKQNKGECKGG+    +D D     +D+EIVY+AFKVFD+NGDGFISC+ELENVL
Sbjct: 63  FYESMSKQNKGECKGGLVDEHEDED-----EDVEIVYMAFKVFDVNGDGFISCEELENVL 122

Query: 122 VKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
            +LELWDA+RS+VD CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 123 SRLELWDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of CsaV3_6G034010 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 258.5 bits (659), Expect = 4.1e-65
Identity = 129/162 (79.63%), Postives = 144/162 (88.89%), Query Frame = 0

Query: 3   PISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGLIC+QEL WLLD+IGIQLTMEELESFLE+PSLDFDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENVLV 122
           YESISKQNKGECKGG+     D   D+ ++D+EIVY+AFKVFD+NGDGFISC+ELENVL 
Sbjct: 64  YESISKQNKGECKGGLV----DEHGDE-EEDVEIVYMAFKVFDVNGDGFISCEELENVLS 123

Query: 123 KLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
           +LELWDA+RS+VD CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 124 RLELWDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of CsaV3_6G034010 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-28
Identity = 77/166 (46.39%), Postives = 98/166 (59.04%), Query Frame = 0

Query: 4   ISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIG-IQLTMEELESFLERPSLDFDEFLFF 63
           I++ DL R+FK LDKN DGL+ L EL W+LD +G  + T +ELE  + + SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENVLV 123
           Y      +KG  K      V DND        E +  AF VFD+NGDG+IS +EL +VL 
Sbjct: 66  YYDAVLDSKGSKKN--IDVVADND--------EAIARAFNVFDVNGDGYISAEELRDVLE 125

Query: 124 KL------ELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMML 163
           +L      + WD        C  MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 RLGFEEEAKAWD--------CGRMIRVHDKNLDGFVDFEEFKNMIL 153

BLAST of CsaV3_6G034010 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.2e-19
Identity = 61/166 (36.75%), Postives = 95/166 (57.23%), Query Frame = 0

Query: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLE------RPSLD 61
           P +++  L RIF   DKN DG I ++EL+  L  +G+   + +L+S +E         L+
Sbjct: 23  PSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLN 82

Query: 62  FDEFLFFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCD 121
           FD+F   ++++     G   GG  G  +D+ +   +++ ++   AFKVFD NGDGFIS  
Sbjct: 83  FDDFSSLHKTLDDSFFGGACGG--GENEDDPSSAAENESDLAE-AFKVFDENGDGFISAR 142

Query: 122 ELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           EL+ VL KL L +    +++    MI + D N DG++DF EFKNMM
Sbjct: 143 ELQTVLKKLGLPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

BLAST of CsaV3_6G034010 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.7e-18
Identity = 59/166 (35.54%), Postives = 92/166 (55.42%), Query Frame = 0

Query: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFL------ERPSLD 61
           P +++  LHR+F   DKN DG I ++EL+  L  +G+     +L+S +      ++  L 
Sbjct: 22  PSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLR 81

Query: 62  FDEFLFFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCD 121
           FD+F   ++++ +       GG   C   +     + D+E    AF VFD +GDGFIS  
Sbjct: 82  FDDFAALHKTLDE----SFFGGEGSCCDGSP----ESDLE---EAFNVFDEDGDGFISAV 141

Query: 122 ELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           EL+ VL KL L +A   +++    MI + D+N DG++DF EFKNMM
Sbjct: 142 ELQKVLKKLGLPEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

BLAST of CsaV3_6G034010 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-17
Identity = 58/164 (35.37%), Postives = 84/164 (51.22%), Query Frame = 0

Query: 4   ISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLER------PSLDFD 63
           + S +L ++FK  DKN DG I  +EL     + GI +  +EL++ +++        +D +
Sbjct: 1   MDSTELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVE 60

Query: 64  EFLFFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDEL 123
           EF   Y SI   +        A      +     D+ E +  AF VFD NGDGFI+ DEL
Sbjct: 61  EFGLLYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDEGMREAFNVFDQNGDGFITVDEL 120

Query: 124 ENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
            +VL  L L     +  D CR MI   D + DG++DF+EFK MM
Sbjct: 121 RSVLSSLGLKHGRTA--DDCRRMISMVDADGDGRVDFKEFKQMM 162

BLAST of CsaV3_6G034010 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.3e-17
Identity = 60/168 (35.71%), Postives = 92/168 (54.76%), Query Frame = 0

Query: 9   LHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLE------RPSLDFDEFLFF 68
           L RIF   DKN DG+I + EL+  L+ +G++  + ELES ++         L F++F+  
Sbjct: 41  LRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISL 100

Query: 69  YESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLA---------FKVFDMNGDGFIS 128
           ++S++  +     GG        D D  ++DM    L+         FKVFD +GDG+IS
Sbjct: 101 HQSLN--DSYFAYGG-------EDEDDNEEDMRKSILSQEEADSFGGFKVFDEDGDGYIS 160

Query: 129 CDELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
             EL+ VL KL    +  S++D    MI + D+N DG++DF EFK+MM
Sbjct: 161 ARELQMVLGKLGF--SEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMM 197

BLAST of CsaV3_6G034010 vs. ExPASy TrEMBL
Match: A0A0A0KFQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 6.1e-91
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

BLAST of CsaV3_6G034010 vs. ExPASy TrEMBL
Match: A0A5D3B6U1 (Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold282G00080 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 4.8e-80
Identity = 148/165 (89.70%), Postives = 156/165 (94.55%), Query Frame = 0

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           M PISSKDLHRIFKKLDKNCDGL+CLQEL WLLD IGIQLTMEELESFLE+PSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQD+     +DD EIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDH----EDDTEIVYMAFKVFDMNGDGFISCDELENV 120

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           LV+LELWDASRS+VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of CsaV3_6G034010 vs. ExPASy TrEMBL
Match: A0A1S3B3T2 (probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 4.8e-80
Identity = 148/165 (89.70%), Postives = 156/165 (94.55%), Query Frame = 0

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           M PISSKDLHRIFKKLDKNCDGL+CLQEL WLLD IGIQLTMEELESFLE+PSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQD+     +DD EIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDH----EDDTEIVYMAFKVFDMNGDGFISCDELENV 120

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           LV+LELWDASRS+VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of CsaV3_6G034010 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.5e-65
Identity = 128/163 (78.53%), Postives = 143/163 (87.73%), Query Frame = 0

Query: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFLF 61
           PPISS DL RIF +LDKN DGLIC+QEL WLLD+IGIQLTMEELESFLE+PSLDFDEFL 
Sbjct: 3   PPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLI 62

Query: 62  FYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENVL 121
           FYES+SKQNKGECKGG+    +D D     +D+EIVY+AFKVFD+NGDGFISC+ELENVL
Sbjct: 63  FYESMSKQNKGECKGGLVDEHEDED-----EDVEIVYMAFKVFDVNGDGFISCEELENVL 122

Query: 122 VKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
            +LELWDA+RS+VD CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 123 SRLELWDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of CsaV3_6G034010 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 4.4e-65
Identity = 128/162 (79.01%), Postives = 144/162 (88.89%), Query Frame = 0

Query: 3   PISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGLIC+QEL WLLD+IGIQLTMEELESFLE+PSLDFDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENVLV 122
           YESISKQNKGECKGG+     D   D+ ++D+EIVY+AFKVFD+NGDGFISC+ELENVL 
Sbjct: 64  YESISKQNKGECKGGLV----DEHGDE-EEDVEIVYMAFKVFDVNGDGFISCEELENVLS 123

Query: 123 KLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
           +LELWDA++S+VD CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 124 RLELWDANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of CsaV3_6G034010 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 127.9 bits (320), Expect = 7.7e-30
Identity = 77/166 (46.39%), Postives = 98/166 (59.04%), Query Frame = 0

Query: 4   ISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIG-IQLTMEELESFLERPSLDFDEFLFF 63
           I++ DL R+FK LDKN DGL+ L EL W+LD +G  + T +ELE  + + SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENVLV 123
           Y      +KG  K      V DND        E +  AF VFD+NGDG+IS +EL +VL 
Sbjct: 66  YYDAVLDSKGSKKN--IDVVADND--------EAIARAFNVFDVNGDGYISAEELRDVLE 125

Query: 124 KL------ELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMML 163
           +L      + WD        C  MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 RLGFEEEAKAWD--------CGRMIRVHDKNLDGFVDFEEFKNMIL 153

BLAST of CsaV3_6G034010 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 97.8 bits (242), Expect = 8.6e-21
Identity = 61/166 (36.75%), Postives = 95/166 (57.23%), Query Frame = 0

Query: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLE------RPSLD 61
           P +++  L RIF   DKN DG I ++EL+  L  +G+   + +L+S +E         L+
Sbjct: 23  PSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLN 82

Query: 62  FDEFLFFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCD 121
           FD+F   ++++     G   GG  G  +D+ +   +++ ++   AFKVFD NGDGFIS  
Sbjct: 83  FDDFSSLHKTLDDSFFGGACGG--GENEDDPSSAAENESDLAE-AFKVFDENGDGFISAR 142

Query: 122 ELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           EL+ VL KL L +    +++    MI + D N DG++DF EFKNMM
Sbjct: 143 ELQTVLKKLGLPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

BLAST of CsaV3_6G034010 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 94.0 bits (232), Expect = 1.2e-19
Identity = 59/166 (35.54%), Postives = 92/166 (55.42%), Query Frame = 0

Query: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFL------ERPSLD 61
           P +++  LHR+F   DKN DG I ++EL+  L  +G+     +L+S +      ++  L 
Sbjct: 22  PSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLR 81

Query: 62  FDEFLFFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCD 121
           FD+F   ++++ +       GG   C   +     + D+E    AF VFD +GDGFIS  
Sbjct: 82  FDDFAALHKTLDE----SFFGGEGSCCDGSP----ESDLE---EAFNVFDEDGDGFISAV 141

Query: 122 ELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           EL+ VL KL L +A   +++    MI + D+N DG++DF EFKNMM
Sbjct: 142 ELQKVLKKLGLPEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

BLAST of CsaV3_6G034010 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 86.3 bits (212), Expect = 2.6e-17
Identity = 56/160 (35.00%), Postives = 87/160 (54.37%), Query Frame = 0

Query: 8   DLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLER------PSLDFDEFLF 67
           +L R+F+  DKN DG I  +EL+  L S+GI +  +EL   +E+        +D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 68  FYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENVL 127
            Y++I                  ++ D+ ++DM+    AF VFD NGDGFI+ DEL+ VL
Sbjct: 65  LYKTIM-----------------DEEDEEEEDMK---EAFNVFDQNGDGFITVDELKAVL 124

Query: 128 VKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
             L L       +D C+ MI+  D + DG+++++EF+ MM
Sbjct: 125 SSLGL--KQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

BLAST of CsaV3_6G034010 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 85.1 bits (209), Expect = 5.7e-17
Identity = 54/164 (32.93%), Postives = 87/164 (53.05%), Query Frame = 0

Query: 4   ISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLER------PSLDFD 63
           I   +L R+F+  DKN DG I  +ELN  L+++GI +  ++L   + +        +D D
Sbjct: 61  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 120

Query: 64  EFLFFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDEL 123
           EF   Y SI  ++              ND +  ++DM+    AF VFD +GDGFI+ +EL
Sbjct: 121 EFESLYSSIVDEH-------------HNDGETEEEDMKD---AFNVFDQDGDGFITVEEL 180

Query: 124 ENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           ++V+  L L       +D C+ MI   D + DG+++++EF  MM
Sbjct: 181 KSVMASLGL--KQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004149438.11.3e-90100.00probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetica... [more]
XP_008441608.11.0e-7989.70PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 put... [more]
XP_038885742.11.3e-7183.03probable calcium-binding protein CML44 [Benincasa hispida][more]
XP_022993348.13.1e-6578.53probable calcium-binding protein CML44 [Cucurbita maxima][more]
XP_023551499.14.1e-6579.63probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LPK51.1e-2846.39Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ... [more]
Q9SVG91.2e-1936.75Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1[more]
Q9FI191.7e-1835.54Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ... [more]
Q2QVI11.1e-1735.37Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
P431874.3e-1735.71Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KFQ66.1e-91100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1[more]
A0A5D3B6U14.8e-8089.70Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B3T24.8e-8089.70probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 P... [more]
A0A6J1K1Y71.5e-6578.53probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893... [more]
A0A6J1FLU54.4e-6579.01probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
AT1G21550.17.7e-3046.39Calcium-binding EF-hand family protein [more]
AT4G20780.18.6e-2136.75calmodulin like 42 [more]
AT5G44460.11.2e-1935.54calmodulin like 43 [more]
AT1G05990.12.6e-1735.00EF hand calcium-binding protein family [more]
AT2G43290.15.7e-1732.93Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 96..124
e-value: 0.19
score: 20.8
coord: 136..164
e-value: 0.0051
score: 26.0
coord: 8..36
e-value: 0.016
score: 24.4
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 12..29
e-value: 0.01
score: 15.3
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 98..161
e-value: 1.2E-9
score: 38.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 4..39
score: 11.891237
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 132..165
score: 13.648647
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 92..127
score: 12.811786
IPR002048EF-hand domainCDDcd00051EFhcoord: 100..161
e-value: 1.09494E-12
score: 57.9429
IPR002048EF-hand domainCDDcd00051EFhcoord: 8..60
e-value: 1.63502E-7
score: 44.0757
NoneNo IPR availableGENE3D1.10.238.10coord: 4..64
e-value: 4.4E-9
score: 38.3
NoneNo IPR availableGENE3D1.10.238.10coord: 65..161
e-value: 8.3E-19
score: 69.7
NoneNo IPR availablePANTHERPTHR10891:SF788CALCIUM-BINDING PROTEIN CML44-RELATEDcoord: 4..164
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 4..164
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 105..117
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 17..29
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 7..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G034010.1CsaV3_6G034010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding