CsaV3_6G000580 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_6G000580 vs. NCBI nr
Match: XP_031743838.1 (U-box domain-containing protein 33 [Cucumis sativus] >KGN45661.1 hypothetical protein Csa_004782 [Cucumis sativus])

HSP 1 Score: 1612.8 bits (4175), Expect = 0.0e+00
Identity = 813/813 (100.00%), Postives = 813/813 (100.00%), Query Frame = 0

Query: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60
           MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS
Sbjct: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60

Query: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120
           MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS
Sbjct: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120

Query: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180
           LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS
Sbjct: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180

Query: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASGSPFERSYATSPS 240
           PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASGSPFERSYATSPS
Sbjct: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASGSPFERSYATSPS 240

Query: 241 SGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIA 300
           SGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIA
Sbjct: 241 SGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIA 300

Query: 301 DAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDD 360
           DAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDD
Sbjct: 301 DAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDD 360

Query: 361 VKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQ 420
           VKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQ
Sbjct: 361 VKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQ 420

Query: 421 RDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGL 480
           RDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGL
Sbjct: 421 RDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGL 480

Query: 481 LYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNL 540
           LYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNL
Sbjct: 481 LYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNL 540

Query: 541 EDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKL 600
           EDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKL
Sbjct: 541 EDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKL 600

Query: 601 AGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRM 660
           AGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRM
Sbjct: 601 AGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRM 660

Query: 661 KEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEP 720
           KEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEP
Sbjct: 661 KEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEP 720

Query: 721 MRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH 780
           MRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH
Sbjct: 721 MRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH 780

Query: 781 DTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814
           DTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 DTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 813

BLAST of CsaV3_6G000580 vs. NCBI nr
Match: XP_008458227.1 (PREDICTED: U-box domain-containing protein 33-like [Cucumis melo] >TYK03015.1 U-box domain-containing protein 33-like [Cucumis melo var. makuwa])

HSP 1 Score: 1498.0 bits (3877), Expect = 0.0e+00
Identity = 758/815 (93.01%), Postives = 782/815 (95.95%), Query Frame = 0

Query: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60
           MAGS GED VYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS
Sbjct: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60

Query: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120
           M+EEEVNAYRELERQDA+KILDVFLLLCHQAGA+AEKLYFESDKIKKGIVELVSLHGIRS
Sbjct: 61  MEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDKIKKGIVELVSLHGIRS 120

Query: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180
           LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQE HADIS
Sbjct: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQEAHADIS 180

Query: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240
           PSSPQSQNINGVSWRT+QFG FNGRISNSP+IVMERLTISETISNASG  SPFERSYATS
Sbjct: 181 PSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLTISETISNASGTWSPFERSYATS 240

Query: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKA 300
           PSSG  DVASSR+EED+YGLGL+SPL LTNFA  TS PH SGFQQDG+A+DSLYIQLEKA
Sbjct: 241 PSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTS-PHLSGFQQDGNANDSLYIQLEKA 300

Query: 301 IADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREEL 360
           I DAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRK+AEE LSKEREEL
Sbjct: 301 ITDAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKEAEEALSKEREEL 360

Query: 361 DDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELL 420
           D+VKNQVNEMMK+LQIA+NN LKLENQIAQSDEMVKELEQKILSAI+LLHNYKNDRDELL
Sbjct: 361 DNVKNQVNEMMKDLQIAQNNELKLENQIAQSDEMVKELEQKILSAIQLLHNYKNDRDELL 420

Query: 421 KQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYK 480
           KQRDEAL+ELDDIR RQVEA SQ SAQLISEFSFSEIVEATRKFDPSLKIV   NGSMYK
Sbjct: 421 KQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATRKFDPSLKIVEGENGSMYK 480

Query: 481 GLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNG 540
           GLLYNTEVSIKMLCSHNLQNPV+FQREVDALSKLRHPN+ATLIGVCPEACILVYDYFPNG
Sbjct: 481 GLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGVCPEACILVYDYFPNG 540

Query: 541 NLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVP 600
           NLEDRLACKDNSSPLSWKTRILIAT LCSALIFIHSNKICK VHGDMKPSN+LLDANYVP
Sbjct: 541 NLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHGDMKPSNVLLDANYVP 600

Query: 601 KLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYL 660
           KLAGFG CHFLTHDEK SYNENLSVG+DA  NHEFPLTT  D+YSFG VLLSLLTG+SYL
Sbjct: 601 KLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTTAFDVYSFGMVLLSLLTGQSYL 660

Query: 661 RMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVL 720
           RM+EDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCC+TDS YRPDLVSD WRVL
Sbjct: 661 RMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCNTDSTYRPDLVSDAWRVL 720

Query: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780
           EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS
Sbjct: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780

Query: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814
           GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814

BLAST of CsaV3_6G000580 vs. NCBI nr
Match: XP_038907095.1 (U-box domain-containing protein 33-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1395.6 bits (3611), Expect = 0.0e+00
Identity = 716/818 (87.53%), Postives = 745/818 (91.08%), Query Frame = 0

Query: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60
           MAGSS ED+VYVAVGNDI  CKLNLVYAIK SGGR+ICILHVHEPAK IPILGTKFLASS
Sbjct: 1   MAGSSEEDVVYVAVGNDIKACKLNLVYAIKQSGGRKICILHVHEPAKLIPILGTKFLASS 60

Query: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120
           MKEE+VNAYRELERQDA+KILD FLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIR 
Sbjct: 61  MKEEKVNAYRELERQDANKILDGFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRR 120

Query: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180
           L+MGAAADKY+SRKMTKIRSRKAMYVHLNA A CQIQFVC GQLIRIREACPQE HADIS
Sbjct: 121 LIMGAAADKYHSRKMTKIRSRKAMYVHLNAPAFCQIQFVCNGQLIRIREACPQEPHADIS 180

Query: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240
             SPQSQNING SWR  Q G FNGRI +SP+I MERLTIS+ I N SG  S FE SY+TS
Sbjct: 181 LPSPQSQNINGASWRPVQSGQFNGRIIDSPSIGMERLTISDAILNTSGTWSAFEHSYSTS 240

Query: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKA 300
           PSSG MDVASSRTEED + LGL+SP  LTNFA  +SPPH  GFQQDGSADDSLYIQLEKA
Sbjct: 241 PSSGCMDVASSRTEEDEFELGLDSPRQLTNFAPNSSPPHLLGFQQDGSADDSLYIQLEKA 300

Query: 301 IADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREEL 360
           I DAANARREAFREALKRAKAE EL  AI  AKV+E LYAEE RGRK+ EE  SKEREEL
Sbjct: 301 ITDAANARREAFREALKRAKAEIELGHAIRRAKVAEALYAEEFRGRKETEEARSKEREEL 360

Query: 361 DDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELL 420
           DDVKNQVNEM KELQIARN GLKLENQIA+SDEMVKELEQKILSAIELLHNYKNDRDELL
Sbjct: 361 DDVKNQVNEMTKELQIARNKGLKLENQIAESDEMVKELEQKILSAIELLHNYKNDRDELL 420

Query: 421 KQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYK 480
           KQRDEALKEL+DIRTRQVEA  QHSA    EFSFSEI EATRKFDPSLKIV   NGSMYK
Sbjct: 421 KQRDEALKELNDIRTRQVEARIQHSAH---EFSFSEIAEATRKFDPSLKIVEGTNGSMYK 480

Query: 481 GLLYNTEVSIKMLCSHNLQNPVD-FQREVDALSKLRHPNIATLIGVCPEACILVYDYFPN 540
           GLLYNTEVSIKMLCSHNLQNP D FQREVD LSKLRHPNIATLIGVCPEACILVYDYFPN
Sbjct: 481 GLLYNTEVSIKMLCSHNLQNPEDEFQREVDVLSKLRHPNIATLIGVCPEACILVYDYFPN 540

Query: 541 GNLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYV 600
           GNLEDRLACKDNSSPLSWKTRI IAT+LCSALIFIHSNK+CK +HGDMKPSN+LLDANYV
Sbjct: 541 GNLEDRLACKDNSSPLSWKTRIRIATELCSALIFIHSNKVCKIIHGDMKPSNVLLDANYV 600

Query: 601 PKLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSY 660
           PKLAGFGTCHFL HDEK SYNE+LS GYDAK NHEFPLTTELD++SFG +LLSLLTG+SY
Sbjct: 601 PKLAGFGTCHFLPHDEKSSYNEDLSAGYDAKGNHEFPLTTELDVFSFGMILLSLLTGQSY 660

Query: 661 LRMKEDVQ--IAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVW 720
           LR+KEDVQ  I IKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCC+T+SMYRPDLVSDVW
Sbjct: 661 LRLKEDVQFIINIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCNTNSMYRPDLVSDVW 720

Query: 721 RVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 780
           RVLEPMRASCGGS SVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW
Sbjct: 721 RVLEPMRASCGGSQSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 780

Query: 781 LDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814
           LDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 LDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 815

BLAST of CsaV3_6G000580 vs. NCBI nr
Match: XP_022959086.1 (U-box domain-containing protein 33-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 660/815 (80.98%), Postives = 726/815 (89.08%), Query Frame = 0

Query: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60
           MAGS G+D+VY+AVGND+NEC+LNLVYAIKH GGRRIC++HVHEPAK IP LGT+FLASS
Sbjct: 1   MAGSCGDDVVYIAVGNDVNECRLNLVYAIKHLGGRRICVVHVHEPAKLIPFLGTQFLASS 60

Query: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120
           MKE+EVNAYRE ERQDA+KILD FL LCHQAG  AEKLYFESDKIKKGIVELVSLHGIR 
Sbjct: 61  MKEKEVNAYRERERQDANKILDGFLFLCHQAGVWAEKLYFESDKIKKGIVELVSLHGIRR 120

Query: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180
           L+MGAAADKYYSRKMTKIRSRKAMYVHL A A CQIQFVC GQLIR REACPQE H D+ 
Sbjct: 121 LIMGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDVP 180

Query: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240
           P SPQSQN+NG  WR  + G FNG    SP+IV+ERL+IS+ +SN SG  SPFERSY+ S
Sbjct: 181 PPSPQSQNVNGACWRPMESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPS 240

Query: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKA 300
            SSG +D ASSRTEED YG+GLNSPLLL + A  +SP H SGFQQD SADD LYIQLEK+
Sbjct: 241 SSSGCLDAASSRTEEDEYGVGLNSPLLLKDVAPNSSPLHLSGFQQDESADDILYIQLEKS 300

Query: 301 IADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREEL 360
           I +AANA+REAFREA+KRA+AE EL+ AI  AK  E LYA E+R RKD EE LSKEREEL
Sbjct: 301 ITEAANAKREAFREAVKRAQAENELENAIFRAKFYEDLYAGEARERKDVEEALSKEREEL 360

Query: 361 DDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELL 420
           ++VKNQVN+MM+ELQ++RN GL+LENQIA SD+MVKELEQKILSAIELLHNYKNDRDELL
Sbjct: 361 ENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDELL 420

Query: 421 KQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYK 480
           KQRDEALKE+DDIRTRQVEA ++H+AQ+ SEFSFSEI EATRKFDPSLKI    +GSMYK
Sbjct: 421 KQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYK 480

Query: 481 GLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNG 540
           GLLYNTEV IKM  SHNLQNPV+FQREVD LS LRHPNIATLIGVCPEACILVYDYFPNG
Sbjct: 481 GLLYNTEVCIKMFGSHNLQNPVEFQREVDVLSILRHPNIATLIGVCPEACILVYDYFPNG 540

Query: 541 NLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVP 600
           +LEDRLACKDNSSPLSWKTRI IA++LCSALIFIHSNKICK +HGD+KP N+LLDANYVP
Sbjct: 541 SLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVP 600

Query: 601 KLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYL 660
           KLAGFG+CHFL HDEKLSYNEN+S  Y  K N EFPLTT+LD++SFG VLLSLLTGKSYL
Sbjct: 601 KLAGFGSCHFLPHDEKLSYNENISAKYGVKSNPEFPLTTKLDVFSFGMVLLSLLTGKSYL 660

Query: 661 RMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVL 720
           RMKEDVQ AI++RKLKDVLDPRAGDWPFVQAEQLAQLALRCC+ +SMYRPDLVSDVWRVL
Sbjct: 661 RMKEDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVL 720

Query: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780
           EPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDS
Sbjct: 721 EPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780

Query: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814
           GH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 GHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 815

BLAST of CsaV3_6G000580 vs. NCBI nr
Match: KAG7013740.1 (U-box domain-containing protein 33 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 660/815 (80.98%), Postives = 724/815 (88.83%), Query Frame = 0

Query: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60
           MAGS G+D+VY+AVGND+NEC+LNLVYAIKH GGRRIC++HVHEPAK IP LGT+FLASS
Sbjct: 1   MAGSCGDDVVYMAVGNDVNECRLNLVYAIKHLGGRRICVVHVHEPAKLIPFLGTQFLASS 60

Query: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120
           MKE+EVNAYRE ERQDA+ ILD FL LCHQAG  AEKLYFESDKIKKGIVELVSLHGIR 
Sbjct: 61  MKEKEVNAYRERERQDANNILDGFLFLCHQAGVWAEKLYFESDKIKKGIVELVSLHGIRR 120

Query: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180
           L+MGAAADKYYSRKMTKIRSRKAMYVHL A A CQIQFVC GQLIR REACPQE H D+ 
Sbjct: 121 LIMGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDVP 180

Query: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240
           P SPQSQN+NG  WR  + G FNG    SP+IV+ERL+IS+ +SN SG  SPFERSY+ S
Sbjct: 181 PPSPQSQNVNGACWRPMESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPS 240

Query: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKA 300
            SSG +D ASSRTEED YG+GLNSPLLL + A  +SP H SGFQQD SADD LYIQLEK+
Sbjct: 241 SSSGCLDAASSRTEEDEYGVGLNSPLLLKDVAPNSSPLHLSGFQQDESADDILYIQLEKS 300

Query: 301 IADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREEL 360
           I +AANA+REAFREA+KRA+AE EL  AI  AK  E LYA E+R RKD EE LSKEREEL
Sbjct: 301 ITEAANAKREAFREAVKRAQAENELGNAIFRAKFYEDLYAGEARERKDVEEALSKEREEL 360

Query: 361 DDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELL 420
           ++VKNQVN+MM+ELQ++RN GL+LENQIA SD+MVKELEQKILSAIELLHNYKNDRDELL
Sbjct: 361 ENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDELL 420

Query: 421 KQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYK 480
           KQRDEALKE+DDIRTRQVEA S+H+AQ+ SEFSFSEI EATRKFDPSLKI    +GSMYK
Sbjct: 421 KQRDEALKEVDDIRTRQVEARSKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYK 480

Query: 481 GLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNG 540
           GLLYNTEV IKM  SHNLQNPV+FQREVD LS LRHPNIATLIGVCPEACILVYDYFPNG
Sbjct: 481 GLLYNTEVCIKMFGSHNLQNPVEFQREVDVLSILRHPNIATLIGVCPEACILVYDYFPNG 540

Query: 541 NLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVP 600
           +LEDRLACKDNSSPLSWKTRI IA++LCSALIFIHSNKICK +HGD+KP N+LLDANYVP
Sbjct: 541 SLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVP 600

Query: 601 KLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYL 660
           KLAGFG+CHFL HDEKLSYNEN+S  Y  K N EFPLTT+LD++SFG VLLSLLTGKSYL
Sbjct: 601 KLAGFGSCHFLPHDEKLSYNENISAKYGVKSNPEFPLTTKLDVFSFGMVLLSLLTGKSYL 660

Query: 661 RMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVL 720
           RMKEDVQ AI++RKLKDVLDPRAGDWPFVQAEQLAQLALRCC+ +SMYRPDLVSDVWRVL
Sbjct: 661 RMKEDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVL 720

Query: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780
           EPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDS
Sbjct: 721 EPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780

Query: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814
           GH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 GHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 815

BLAST of CsaV3_6G000580 vs. ExPASy Swiss-Prot
Match: Q8GUH1 (U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2 SV=2)

HSP 1 Score: 717.6 bits (1851), Expect = 1.6e-205
Identity = 387/811 (47.72%), Postives = 537/811 (66.21%), Query Frame = 0

Query: 7   EDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASSMKEEEV 66
           ++ ++VAV   + + K  L++A++++GG++IC++HVH+P++ IP++G KF   ++KEEEV
Sbjct: 44  DEKIFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPSQMIPLMGAKFPVGAVKEEEV 103

Query: 67  NAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRSLVMGAA 126
             +RE ER+    ILD +L +C Q G RAEK++ E + I+ GIV+L+S  GIR LVMGAA
Sbjct: 104 RVFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENGIVQLISELGIRKLVMGAA 163

Query: 127 ADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADISPSSPQS 186
           AD++YSR+MT ++SRKA++V   A   CQI F CKG LI  REA   +T ++ +   P  
Sbjct: 164 ADRHYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYLIHTREATMDDTESEYASPRPSI 223

Query: 187 QNINGVSWRTEQFGLFNGRISNSPTI--VMERLTISETISNASGSPFERSYATSPSSGYM 246
                    ++    F+   S    I  V    ++ + +SN S        +T  S    
Sbjct: 224 S-------ASDLLQTFSTPESEHQHISRVQSTDSVQQLVSNGS--------STEQSGRVS 283

Query: 247 DVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIADAAN 306
           D + +  EE+    G      +T  AT  S  H S        DDS  +++ KA ++A +
Sbjct: 284 DGSLNTDEEERESDGSE----VTGSATVMSSGHSSPSSFPDGVDDSFNVKIRKATSEAHS 343

Query: 307 ARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDDVKNQ 366
           +++EAF E L+R KAEK   +AI  AK SE+ Y+EE + RKD E  ++KE+E    +KN+
Sbjct: 344 SKQEAFAETLRRQKAEKNALDAIRRAKQSESAYSEELKRRKDTEIAVAKEKERFITIKNE 403

Query: 367 VNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQRDEA 426
              +M+ELQ A      LE+QIA+SD  +++L QK+  A++LL   +++R+EL  +RD A
Sbjct: 404 QEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKLDIAVKLLQKLRDEREELQTERDRA 463

Query: 427 LKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGLLYNT 486
           L+E +++R+   E  +    Q  ++FSFSEI EAT  FD +LKI     GS+Y GLL +T
Sbjct: 464 LREAEELRS-HAETSTLQLPQYFTDFSFSEIEEATNHFDSTLKIGEGGYGSIYVGLLRHT 523

Query: 487 EVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRL 546
           +V+IKML  ++ Q PV++Q+EVD LSK+RHPNI TLIG CPE   LVY+Y P G+LEDRL
Sbjct: 524 QVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITLIGACPEGWSLVYEYLPGGSLEDRL 583

Query: 547 ACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKLAGFG 606
            CKDNS PLSW+ R+ IAT++C+AL+F+HSNK    VHGD+KP+NILLD+N V KL+ FG
Sbjct: 584 TCKDNSPPLSWQNRVRIATEICAALVFLHSNKAHSLVHGDLKPANILLDSNLVSKLSDFG 643

Query: 607 TCHFLTHDEKLSYNENL--SVGY-DAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRMK 666
           TC  L  +   S   ++  +V Y D + +    LT + D+YSFG +LL LLTG+  LR+ 
Sbjct: 644 TCSLLHPNGSKSVRTDVTGTVAYLDPEASSSGELTPKSDVYSFGIILLRLLTGRPALRIS 703

Query: 667 EDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEPM 726
            +V+ A+    L D+LDP AGDWPFVQAEQLA+LALRCC+T S  RPDL ++VWRVLEPM
Sbjct: 704 NEVKYALDNGTLNDLLDPLAGDWPFVQAEQLARLALRCCETVSENRPDLGTEVWRVLEPM 763

Query: 727 RASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHD 786
           RAS GGS S  L   + ++  PPYFICPIFQEVM+DPHVAADGFTYEAEA+R WLDS HD
Sbjct: 764 RASSGGSSSFHLGRNEHRI-APPYFICPIFQEVMQDPHVAADGFTYEAEAIRAWLDSEHD 823

Query: 787 TSPMTNLRLDHQNLVPNRALRSVIQEWLQQH 813
           TSPMTN++L H +L+ N ALRS IQEWLQ H
Sbjct: 824 TSPMTNVKLSHTSLIANHALRSAIQEWLQHH 833

BLAST of CsaV3_6G000580 vs. ExPASy Swiss-Prot
Match: Q94A51 (U-box domain-containing protein 32 OS=Arabidopsis thaliana OX=3702 GN=PUB32 PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.0e-139
Identity = 298/811 (36.74%), Postives = 467/811 (57.58%), Query Frame = 0

Query: 7   EDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASSMKEEEV 66
           ++ ++VAV  D+   K  +++A ++  G++IC+L+VH  A+       K +  S K+ +V
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDV 73

Query: 67  NAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRSLVMGAA 126
                +E+   D++++ +L L  +   + +KL      I++ IVEL++ H I+ LVMGAA
Sbjct: 74  KVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAA 133

Query: 127 ADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADISPSSPQS 186
           +DK+YS KMT ++S+KA++V   A  SC I F+CKG LI  R +     +    P   Q 
Sbjct: 134 SDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIFTRASNDDSNNRQTMPPLVQL 193

Query: 187 QNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG----SPFERSYATSPSSG 246
            + N    ++E+            + +  RL    ++    G       ER      +  
Sbjct: 194 DSDNETR-KSEKL---------ESSYMRRRLRYWRSLLEQDGEKDTGQLEREKVEPRAPP 253

Query: 247 YMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIADA 306
                SS +  +  G    SP L+ +    TS  +    +++G     ++ + +KA+ D 
Sbjct: 254 LFSSGSSSSFGEPVGPEPVSPELVDSDTLNTS--NVEEKEREGDVARKVH-RYDKAMHDI 313

Query: 307 ANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDDVK 366
             + R  + EA K+ + +    EA+C AK  E L  +ES  RK  EE L KE+ E+  V 
Sbjct: 314 GQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELLEKEKLEVKMVI 373

Query: 367 NQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQRD 426
            Q N  MKELQ+ +   LKLE+Q+ +  ++ KE  +K  +A+ELL +++  RDE+    +
Sbjct: 374 EQNNGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMELLKSFRQKRDEIRIDHE 433

Query: 427 EALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGLLY 486
            A+KE++ +R        + S   + ++SF EI EAT +FDPS K+     GS+YKG L 
Sbjct: 434 NAVKEVNALRRLVKGETGESSGSEMLDYSFMEINEATNEFDPSWKLGEGKYGSVYKGNLQ 493

Query: 487 NTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNLED 546
           + +V++KML S+   N  +F+R V+ LS++RHPN+ TL+G CPE+  L+Y Y PNG+LED
Sbjct: 494 HLQVAVKMLPSYGSLNHFEFERRVEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLED 553

Query: 547 RLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKLAG 606
             + ++N   LSW++RI IA+++CSAL+F+HSN  C  +HG++KPS ILLD+N V K+  
Sbjct: 554 CFSSENNVPALSWESRIRIASEICSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKIND 613

Query: 607 FGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRMKE 666
           +G    +  D     + ++   Y   R     +T E DIY+FG +LL LLT +    +  
Sbjct: 614 YGISQLIPIDGLDKSDPHVDPHYFVSRE----MTLESDIYAFGIILLQLLTRRPVSGILR 673

Query: 667 DVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEPMR 726
           DV+ A++   +  VLD  AGDWP  + ++LA +A+RCC  + M RPDL + V R ++ M+
Sbjct: 674 DVKCALENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRPDL-AVVLRFIDRMK 733

Query: 727 ASCGGSLSVCLSFGDQQV--QPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH 786
           A    S S   S+ +Q V  +PP +++CPIFQEVM+DP +AADGFTYEAEA+R WL +GH
Sbjct: 734 APEVPS-SETSSYANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGH 793

Query: 787 DTSPMTNLRLDHQNLVPNRALRSVIQEWLQQ 812
           DTSPMTNL+++  NL+PN AL   IQ+W  Q
Sbjct: 794 DTSPMTNLKMEDCNLIPNHALHLAIQDWQNQ 804

BLAST of CsaV3_6G000580 vs. ExPASy Swiss-Prot
Match: Q5WA76 (U-box domain-containing protein 70 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB70 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 9.2e-113
Identity = 230/532 (43.23%), Postives = 325/532 (61.09%), Query Frame = 0

Query: 288 DDSLYIQLEKAIADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDA 347
           +D L  +L+K++ +AA  ++EA  E LKR ++E+        A+  E LY  + + RK+ 
Sbjct: 292 NDDLCNKLQKSMDEAAVLKKEASDERLKRIESER-------LARTLEDLYLSQVQQRKET 351

Query: 348 EEELSKEREELDDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELL 407
           EE LS+ ++E + +K Q +E+  ELQ        L  Q++ S E  + L           
Sbjct: 352 EESLSRVQQEFEQLKIQQDEVTVELQRVNEQNENLLGQLSDSREHFEWL----------- 411

Query: 408 HNYKNDRDELLKQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLK 467
               ++ D+LL++RD A++E++++R ++ + +S     +  EFS SE+  AT  F  SLK
Sbjct: 412 ---LSEHDQLLRERDNAVREVEELRQKRGQMLSVLVTAMHCEFSSSEVESATENFSNSLK 471

Query: 468 IVTDANGSMYKGLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEA 527
           I     G +YKG+L N  V+IK+L   +LQ    F++EV  LS++RHP++ TL+G C E+
Sbjct: 472 IGEGGFGCVYKGILRNMTVAIKVLRPDSLQGQSQFEQEVSILSRVRHPHLVTLLGACSES 531

Query: 528 CILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKP 587
             LVY++ PNG+LED L C D    L+W+ RI I  ++CSALIF+H NK    VHGD+KP
Sbjct: 532 STLVYEFLPNGSLEDFLMCSDKRQTLTWQARIRIIAEICSALIFLHKNKPHPVVHGDLKP 591

Query: 588 SNILLDANYVPKLAGFGTCHFL---THDEKLSYNENLSVGYDAKRNHEF----PLTTELD 647
           +NILL  N V KL+ FG    L   + +    Y     VG     + EF     LT + D
Sbjct: 592 ANILLGVNLVSKLSDFGISRLLIQSSTNNTTLYRTMHPVGTPLYMDPEFLSTGELTPQSD 651

Query: 648 IYSFGTVLLSLLTGKSYLRMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCC 707
           +YSFG V+L LLTGK  + +K  V+ A++K  L  V+D   G+WP +  EQLA LALRC 
Sbjct: 652 VYSFGIVVLRLLTGKPPVGIKNIVEDAMEKGDLNSVIDTSVGEWPHLHIEQLAYLALRCT 711

Query: 708 DTDSMYRPDLVSDVWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHV 767
           +     RPDL  +VW ++E +R +   S S   S  DQ   PP YFICPI Q++M+DPH+
Sbjct: 712 ELSRRCRPDLSGEVWAIVEAIRDAALSSPSSSRSAQDQN-SPPSYFICPISQDIMDDPHI 771

Query: 768 AADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQH 813
           AADGFTYEAEA+R WL +GHDTSPMTNL L+H+ L+PNRALRS IQEWLQQH
Sbjct: 772 AADGFTYEAEAIRSWLCNGHDTSPMTNLLLEHEELIPNRALRSAIQEWLQQH 801

BLAST of CsaV3_6G000580 vs. ExPASy Swiss-Prot
Match: Q0DR28 (U-box domain-containing protein 57 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB57 PE=2 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 5.1e-87
Identity = 177/431 (41.07%), Postives = 244/431 (56.61%), Query Frame = 0

Query: 390 DEMVKELEQKILSAIELLHNYKNDRDELLKQRDEALKELDDIR------TRQVEAMSQHS 449
           D    E  +K+    E +   K+  D  L Q+++A+   D ++       +QV  +   S
Sbjct: 81  DRKTYEEVRKVHILEEEIVTLKHQADTYLVQKEKAVTAYDQLKHERDNAVQQVNELRDQS 140

Query: 450 AQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGLLYNTEVSIKMLCSHNLQNPVDFQ 509
             +I +FS  ++ +AT  F  + ++     G  YKG+++N +V IK+  S  L     FQ
Sbjct: 141 THIILDFSRKDMEQATEHFKNAREVGDTEYGHTYKGMIHNMKVLIKLSSSQKL-----FQ 200

Query: 510 REVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIAT 569
           +EV  L + RHPNI T IGVC E   LVY++ PNGNLEDR+ C +NS+PLSW  R  I  
Sbjct: 201 QEVSILRQWRHPNIITFIGVCSEVSALVYEWLPNGNLEDRIICTNNSAPLSWYNRTQIIG 260

Query: 570 QLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKLAGFGTCHFLTHDEKLSYNENLSV 629
           ++C AL+F+HSNK    VHGD++P NIL+DANY  K+  FG  +          N    +
Sbjct: 261 EICCALLFLHSNKSTALVHGDLRPCNILIDANYRSKICNFGMSNLFLQLGTFPPNLTARL 320

Query: 630 GY-DAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRMKEDVQIAIKKRKLKDVLDPRAG 689
            Y D + N    LTT  D+YS G ++L LLTG   L + E V  A+    L  ++D  AG
Sbjct: 321 PYMDPEFNTTGELTTLSDVYSLGVIILRLLTGMPPLTLSEKVAEALGSDSLHLLIDKSAG 380

Query: 690 DWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEPMRASCGGSLSVCLSFGDQQVQP 749
           DWP+++A+QLA + L C       RPDL+++VW V+EP+      +    L         
Sbjct: 381 DWPYIEAKQLALIGLSCTGMTRKKRPDLLNEVWIVIEPLTRKPPAATWPYLQSASGDSSV 440

Query: 750 PPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALR 809
           P  FICPI  E+M+DP VA+DGFTYEAEA+R W D G   SPMTNL L + NLVPNR LR
Sbjct: 441 PAAFICPISMEIMKDPQVASDGFTYEAEAIRCWFDRGISRSPMTNLALPNLNLVPNRVLR 500

Query: 810 SVIQEWLQQHQ 814
           S I  +LQQ Q
Sbjct: 501 SFIHGYLQQQQ 506

BLAST of CsaV3_6G000580 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 281.6 bits (719), Expect = 2.9e-74
Identity = 231/812 (28.45%), Postives = 381/812 (46.92%), Query Frame = 0

Query: 39  ILHVHEPAKFIPI-LGTKFLASSMKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEK 98
           +LH+H     +P  +G     S ++++ V AYR+     ++++L  +  L  +     E 
Sbjct: 53  LLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEV 112

Query: 99  LYFESDKIKKGIVELVSLHGIRSLVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQ 158
           L  ESD +   I E V+   I  +V+G ++  ++SRK        A+  +      C + 
Sbjct: 113 LVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSFFSRKADICSVISALMPNF-----CTVY 172

Query: 159 FVCKGQL-----------IRIREACPQETHADISPSSPQSQNINGVSWRTEQFGLFNGRI 218
            V KG+L             IRE   + T++    S P S + + +S   +         
Sbjct: 173 VVSKGKLSCVRPSDSDGNATIREDGSERTNSSSGSSGPTSDSSDVMSSAHDS-------Q 232

Query: 219 SNSPTIVMERLTISETISNASGSPFERSYATSPSSGYMDVASSRTEEDAYGLGLNSPLLL 278
           S   ++ + R+     I+  +  P E S   S  +  M +  +    D   +  +S    
Sbjct: 233 SRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSL-DAEEARDVSSINRSS---- 292

Query: 279 TNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIADAANARREA-FREALKRAKAEKELDE 338
           T+  ++ +P      ++D       Y + ++A++ +++ R    F      +    +   
Sbjct: 293 TDTTSRWTPR-----RRD-------YEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTH 352

Query: 339 AICWAKVSETLYAEESRGRKDAEEELSKEREELDDVKNQVNEMMKELQ---------IAR 398
           +    + S    A   +   D +  L+ E E+L      V EM    Q         +  
Sbjct: 353 SRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGE 412

Query: 399 NNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRD----------ELLKQRDEALK 458
            N  +LE  I   +  +KE E + L+  E  +  K  RD          E+ ++R+   K
Sbjct: 413 LNQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAERK 472

Query: 459 ELDDIRTRQVEAMSQHSAQL-ISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGLLYNTE 518
              D + ++    +  S QL    F++ EI+ AT  F   LKI   A G++YK  L++T 
Sbjct: 473 SARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTT 532

Query: 519 VSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRLA 578
             +K+L S   Q    FQ+E++ LSK+RHP++  L+G CPE   LVY+Y  NG+LEDRL 
Sbjct: 533 AVVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLF 592

Query: 579 CKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKLAGFGT 638
             +NS PL W  R  IA ++ +AL+F+H +K    +H D+KP+NILLD N+V K+   G 
Sbjct: 593 QVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGL 652

Query: 639 CHFLTHDEKLS----YNENLSVG----YDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSY 698
              +  D   +    Y +   VG     D +      ++++ DIYSFG +LL LLT K  
Sbjct: 653 STMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPA 712

Query: 699 LRMKEDVQIAIKKR-KLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWR 758
           + +   V+ A+    +   +LD +AG+WP  +  +LA LAL C +     RPDL   +  
Sbjct: 713 IALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILP 772

Query: 759 VLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWL 809
            LE ++     + +   SF     QPP +FICP+ ++VM +P VAADG+TY+  A+  WL
Sbjct: 773 ALENLKKVAEKARN---SFSGVSTQPPTHFICPLLKDVMNEPCVAADGYTYDRHAIEEWL 831

BLAST of CsaV3_6G000580 vs. ExPASy TrEMBL
Match: A0A0A0KD01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004520 PE=4 SV=1)

HSP 1 Score: 1612.8 bits (4175), Expect = 0.0e+00
Identity = 813/813 (100.00%), Postives = 813/813 (100.00%), Query Frame = 0

Query: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60
           MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS
Sbjct: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60

Query: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120
           MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS
Sbjct: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120

Query: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180
           LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS
Sbjct: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180

Query: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASGSPFERSYATSPS 240
           PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASGSPFERSYATSPS
Sbjct: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASGSPFERSYATSPS 240

Query: 241 SGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIA 300
           SGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIA
Sbjct: 241 SGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIA 300

Query: 301 DAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDD 360
           DAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDD
Sbjct: 301 DAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDD 360

Query: 361 VKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQ 420
           VKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQ
Sbjct: 361 VKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQ 420

Query: 421 RDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGL 480
           RDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGL
Sbjct: 421 RDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGL 480

Query: 481 LYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNL 540
           LYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNL
Sbjct: 481 LYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNL 540

Query: 541 EDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKL 600
           EDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKL
Sbjct: 541 EDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKL 600

Query: 601 AGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRM 660
           AGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRM
Sbjct: 601 AGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRM 660

Query: 661 KEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEP 720
           KEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEP
Sbjct: 661 KEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEP 720

Query: 721 MRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH 780
           MRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH
Sbjct: 721 MRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH 780

Query: 781 DTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814
           DTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 DTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 813

BLAST of CsaV3_6G000580 vs. ExPASy TrEMBL
Match: A0A5D3BV84 (E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001100 PE=4 SV=1)

HSP 1 Score: 1498.0 bits (3877), Expect = 0.0e+00
Identity = 758/815 (93.01%), Postives = 782/815 (95.95%), Query Frame = 0

Query: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60
           MAGS GED VYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS
Sbjct: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60

Query: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120
           M+EEEVNAYRELERQDA+KILDVFLLLCHQAGA+AEKLYFESDKIKKGIVELVSLHGIRS
Sbjct: 61  MEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDKIKKGIVELVSLHGIRS 120

Query: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180
           LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQE HADIS
Sbjct: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQEAHADIS 180

Query: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240
           PSSPQSQNINGVSWRT+QFG FNGRISNSP+IVMERLTISETISNASG  SPFERSYATS
Sbjct: 181 PSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLTISETISNASGTWSPFERSYATS 240

Query: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKA 300
           PSSG  DVASSR+EED+YGLGL+SPL LTNFA  TS PH SGFQQDG+A+DSLYIQLEKA
Sbjct: 241 PSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTS-PHLSGFQQDGNANDSLYIQLEKA 300

Query: 301 IADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREEL 360
           I DAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRK+AEE LSKEREEL
Sbjct: 301 ITDAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKEAEEALSKEREEL 360

Query: 361 DDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELL 420
           D+VKNQVNEMMK+LQIA+NN LKLENQIAQSDEMVKELEQKILSAI+LLHNYKNDRDELL
Sbjct: 361 DNVKNQVNEMMKDLQIAQNNELKLENQIAQSDEMVKELEQKILSAIQLLHNYKNDRDELL 420

Query: 421 KQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYK 480
           KQRDEAL+ELDDIR RQVEA SQ SAQLISEFSFSEIVEATRKFDPSLKIV   NGSMYK
Sbjct: 421 KQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATRKFDPSLKIVEGENGSMYK 480

Query: 481 GLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNG 540
           GLLYNTEVSIKMLCSHNLQNPV+FQREVDALSKLRHPN+ATLIGVCPEACILVYDYFPNG
Sbjct: 481 GLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGVCPEACILVYDYFPNG 540

Query: 541 NLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVP 600
           NLEDRLACKDNSSPLSWKTRILIAT LCSALIFIHSNKICK VHGDMKPSN+LLDANYVP
Sbjct: 541 NLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHGDMKPSNVLLDANYVP 600

Query: 601 KLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYL 660
           KLAGFG CHFLTHDEK SYNENLSVG+DA  NHEFPLTT  D+YSFG VLLSLLTG+SYL
Sbjct: 601 KLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTTAFDVYSFGMVLLSLLTGQSYL 660

Query: 661 RMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVL 720
           RM+EDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCC+TDS YRPDLVSD WRVL
Sbjct: 661 RMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCNTDSTYRPDLVSDAWRVL 720

Query: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780
           EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS
Sbjct: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780

Query: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814
           GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814

BLAST of CsaV3_6G000580 vs. ExPASy TrEMBL
Match: A0A1S3C7H7 (E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103497714 PE=4 SV=1)

HSP 1 Score: 1498.0 bits (3877), Expect = 0.0e+00
Identity = 758/815 (93.01%), Postives = 782/815 (95.95%), Query Frame = 0

Query: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60
           MAGS GED VYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS
Sbjct: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60

Query: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120
           M+EEEVNAYRELERQDA+KILDVFLLLCHQAGA+AEKLYFESDKIKKGIVELVSLHGIRS
Sbjct: 61  MEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDKIKKGIVELVSLHGIRS 120

Query: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180
           LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQE HADIS
Sbjct: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQEAHADIS 180

Query: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240
           PSSPQSQNINGVSWRT+QFG FNGRISNSP+IVMERLTISETISNASG  SPFERSYATS
Sbjct: 181 PSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLTISETISNASGTWSPFERSYATS 240

Query: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKA 300
           PSSG  DVASSR+EED+YGLGL+SPL LTNFA  TS PH SGFQQDG+A+DSLYIQLEKA
Sbjct: 241 PSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTS-PHLSGFQQDGNANDSLYIQLEKA 300

Query: 301 IADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREEL 360
           I DAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRK+AEE LSKEREEL
Sbjct: 301 ITDAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKEAEEALSKEREEL 360

Query: 361 DDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELL 420
           D+VKNQVNEMMK+LQIA+NN LKLENQIAQSDEMVKELEQKILSAI+LLHNYKNDRDELL
Sbjct: 361 DNVKNQVNEMMKDLQIAQNNELKLENQIAQSDEMVKELEQKILSAIQLLHNYKNDRDELL 420

Query: 421 KQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYK 480
           KQRDEAL+ELDDIR RQVEA SQ SAQLISEFSFSEIVEATRKFDPSLKIV   NGSMYK
Sbjct: 421 KQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATRKFDPSLKIVEGENGSMYK 480

Query: 481 GLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNG 540
           GLLYNTEVSIKMLCSHNLQNPV+FQREVDALSKLRHPN+ATLIGVCPEACILVYDYFPNG
Sbjct: 481 GLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGVCPEACILVYDYFPNG 540

Query: 541 NLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVP 600
           NLEDRLACKDNSSPLSWKTRILIAT LCSALIFIHSNKICK VHGDMKPSN+LLDANYVP
Sbjct: 541 NLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHGDMKPSNVLLDANYVP 600

Query: 601 KLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYL 660
           KLAGFG CHFLTHDEK SYNENLSVG+DA  NHEFPLTT  D+YSFG VLLSLLTG+SYL
Sbjct: 601 KLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTTAFDVYSFGMVLLSLLTGQSYL 660

Query: 661 RMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVL 720
           RM+EDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCC+TDS YRPDLVSD WRVL
Sbjct: 661 RMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCNTDSTYRPDLVSDAWRVL 720

Query: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780
           EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS
Sbjct: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780

Query: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814
           GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814

BLAST of CsaV3_6G000580 vs. ExPASy TrEMBL
Match: A0A6J1H3V9 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1)

HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 660/815 (80.98%), Postives = 726/815 (89.08%), Query Frame = 0

Query: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60
           MAGS G+D+VY+AVGND+NEC+LNLVYAIKH GGRRIC++HVHEPAK IP LGT+FLASS
Sbjct: 1   MAGSCGDDVVYIAVGNDVNECRLNLVYAIKHLGGRRICVVHVHEPAKLIPFLGTQFLASS 60

Query: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120
           MKE+EVNAYRE ERQDA+KILD FL LCHQAG  AEKLYFESDKIKKGIVELVSLHGIR 
Sbjct: 61  MKEKEVNAYRERERQDANKILDGFLFLCHQAGVWAEKLYFESDKIKKGIVELVSLHGIRR 120

Query: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180
           L+MGAAADKYYSRKMTKIRSRKAMYVHL A A CQIQFVC GQLIR REACPQE H D+ 
Sbjct: 121 LIMGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDVP 180

Query: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240
           P SPQSQN+NG  WR  + G FNG    SP+IV+ERL+IS+ +SN SG  SPFERSY+ S
Sbjct: 181 PPSPQSQNVNGACWRPMESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPS 240

Query: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKA 300
            SSG +D ASSRTEED YG+GLNSPLLL + A  +SP H SGFQQD SADD LYIQLEK+
Sbjct: 241 SSSGCLDAASSRTEEDEYGVGLNSPLLLKDVAPNSSPLHLSGFQQDESADDILYIQLEKS 300

Query: 301 IADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREEL 360
           I +AANA+REAFREA+KRA+AE EL+ AI  AK  E LYA E+R RKD EE LSKEREEL
Sbjct: 301 ITEAANAKREAFREAVKRAQAENELENAIFRAKFYEDLYAGEARERKDVEEALSKEREEL 360

Query: 361 DDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELL 420
           ++VKNQVN+MM+ELQ++RN GL+LENQIA SD+MVKELEQKILSAIELLHNYKNDRDELL
Sbjct: 361 ENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDELL 420

Query: 421 KQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYK 480
           KQRDEALKE+DDIRTRQVEA ++H+AQ+ SEFSFSEI EATRKFDPSLKI    +GSMYK
Sbjct: 421 KQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYK 480

Query: 481 GLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNG 540
           GLLYNTEV IKM  SHNLQNPV+FQREVD LS LRHPNIATLIGVCPEACILVYDYFPNG
Sbjct: 481 GLLYNTEVCIKMFGSHNLQNPVEFQREVDVLSILRHPNIATLIGVCPEACILVYDYFPNG 540

Query: 541 NLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVP 600
           +LEDRLACKDNSSPLSWKTRI IA++LCSALIFIHSNKICK +HGD+KP N+LLDANYVP
Sbjct: 541 SLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVP 600

Query: 601 KLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYL 660
           KLAGFG+CHFL HDEKLSYNEN+S  Y  K N EFPLTT+LD++SFG VLLSLLTGKSYL
Sbjct: 601 KLAGFGSCHFLPHDEKLSYNENISAKYGVKSNPEFPLTTKLDVFSFGMVLLSLLTGKSYL 660

Query: 661 RMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVL 720
           RMKEDVQ AI++RKLKDVLDPRAGDWPFVQAEQLAQLALRCC+ +SMYRPDLVSDVWRVL
Sbjct: 661 RMKEDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVL 720

Query: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780
           EPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDS
Sbjct: 721 EPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780

Query: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814
           GH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 GHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 815

BLAST of CsaV3_6G000580 vs. ExPASy TrEMBL
Match: A0A6J1L2C9 (E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111499216 PE=4 SV=1)

HSP 1 Score: 1316.2 bits (3405), Expect = 0.0e+00
Identity = 660/815 (80.98%), Postives = 723/815 (88.71%), Query Frame = 0

Query: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60
           MAGS G+D+VY+AVGND+NECKLNLVYA+KHSGGRRICILHVHEPAK IP LGT+FLASS
Sbjct: 1   MAGSCGDDVVYMAVGNDVNECKLNLVYALKHSGGRRICILHVHEPAKLIPFLGTQFLASS 60

Query: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120
           MKE+EVNAYRE ERQDA+KILD FL LCHQAG RAEKLYFESDKIKKGIVELVSLHGIR 
Sbjct: 61  MKEKEVNAYRERERQDANKILDGFLFLCHQAGVRAEKLYFESDKIKKGIVELVSLHGIRR 120

Query: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180
           L+MGAAADKYYSRKMTKIRSRKAMYVHL A A CQIQFVC GQLIR REACPQE H DI 
Sbjct: 121 LIMGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIP 180

Query: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240
           P SPQSQN+NG  WR  + G FNG    SP+IV+ERL+IS+ + N SG  SP ERSY+ S
Sbjct: 181 PPSPQSQNVNGACWRPVEPGQFNGSRIQSPSIVLERLSISKLVLNDSGTSSPSERSYSPS 240

Query: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKA 300
            SSG +D A SRTEEDAYG+GLNSPLLL + A  +SP H  GFQQD SADD LYIQLEK+
Sbjct: 241 SSSGCLDAACSRTEEDAYGVGLNSPLLLKDVAPNSSPLHLLGFQQDESADDILYIQLEKS 300

Query: 301 IADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREEL 360
           I +AANA+REAFREA+KRA+AE EL  AI  AK  E LYA E+R RKD EE LSKEREE 
Sbjct: 301 ITEAANAKREAFREAVKRAQAENELGNAIFRAKFYEDLYAGEARERKDVEEALSKEREEF 360

Query: 361 DDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELL 420
           ++VKNQVN+MM+ELQ+++N GL+LENQIA SD+MVKELEQKILSAIELLHNYKNDRDELL
Sbjct: 361 ENVKNQVNKMMEELQVSQNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDELL 420

Query: 421 KQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYK 480
           KQRDEALKE+DDIRTRQVEA ++H+AQ+ SEFSFSEI EATRKFDPSLKI    +GSMYK
Sbjct: 421 KQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYK 480

Query: 481 GLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNG 540
           GLLYNTEV IKM  SHNLQNPV+FQREVDALS +RHPNIATLIGVCPEACILVYDYFPNG
Sbjct: 481 GLLYNTEVCIKMFGSHNLQNPVEFQREVDALSIMRHPNIATLIGVCPEACILVYDYFPNG 540

Query: 541 NLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVP 600
           +LEDRLACKDNSSPLSWKTRI IA+ LCSALIFIHSNKICK +HGD+KP N+LLDANYVP
Sbjct: 541 SLEDRLACKDNSSPLSWKTRIRIASDLCSALIFIHSNKICKIIHGDLKPLNVLLDANYVP 600

Query: 601 KLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYL 660
           KLAGFG+CH L HDEKLSYNEN+S  Y  K NHEFPLTT+LD++SFG VLLSLLTGKSYL
Sbjct: 601 KLAGFGSCHLLPHDEKLSYNENISAKYGVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYL 660

Query: 661 RMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVL 720
           RMKEDVQ AI++RKLKDVLDPRAGDWPFVQAEQLAQLALRCC+ +SMYRPDLVSDVWRVL
Sbjct: 661 RMKEDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVL 720

Query: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780
           EPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDS
Sbjct: 721 EPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780

Query: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814
           GH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 GHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 815

BLAST of CsaV3_6G000580 vs. TAIR 10
Match: AT2G45910.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 717.6 bits (1851), Expect = 1.1e-206
Identity = 387/811 (47.72%), Postives = 537/811 (66.21%), Query Frame = 0

Query: 7   EDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASSMKEEEV 66
           ++ ++VAV   + + K  L++A++++GG++IC++HVH+P++ IP++G KF   ++KEEEV
Sbjct: 44  DEKIFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPSQMIPLMGAKFPVGAVKEEEV 103

Query: 67  NAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRSLVMGAA 126
             +RE ER+    ILD +L +C Q G RAEK++ E + I+ GIV+L+S  GIR LVMGAA
Sbjct: 104 RVFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENGIVQLISELGIRKLVMGAA 163

Query: 127 ADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADISPSSPQS 186
           AD++YSR+MT ++SRKA++V   A   CQI F CKG LI  REA   +T ++ +   P  
Sbjct: 164 ADRHYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYLIHTREATMDDTESEYASPRPSI 223

Query: 187 QNINGVSWRTEQFGLFNGRISNSPTI--VMERLTISETISNASGSPFERSYATSPSSGYM 246
                    ++    F+   S    I  V    ++ + +SN S        +T  S    
Sbjct: 224 S-------ASDLLQTFSTPESEHQHISRVQSTDSVQQLVSNGS--------STEQSGRVS 283

Query: 247 DVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIADAAN 306
           D + +  EE+    G      +T  AT  S  H S        DDS  +++ KA ++A +
Sbjct: 284 DGSLNTDEEERESDGSE----VTGSATVMSSGHSSPSSFPDGVDDSFNVKIRKATSEAHS 343

Query: 307 ARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDDVKNQ 366
           +++EAF E L+R KAEK   +AI  AK SE+ Y+EE + RKD E  ++KE+E    +KN+
Sbjct: 344 SKQEAFAETLRRQKAEKNALDAIRRAKQSESAYSEELKRRKDTEIAVAKEKERFITIKNE 403

Query: 367 VNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQRDEA 426
              +M+ELQ A      LE+QIA+SD  +++L QK+  A++LL   +++R+EL  +RD A
Sbjct: 404 QEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKLDIAVKLLQKLRDEREELQTERDRA 463

Query: 427 LKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGLLYNT 486
           L+E +++R+   E  +    Q  ++FSFSEI EAT  FD +LKI     GS+Y GLL +T
Sbjct: 464 LREAEELRS-HAETSTLQLPQYFTDFSFSEIEEATNHFDSTLKIGEGGYGSIYVGLLRHT 523

Query: 487 EVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRL 546
           +V+IKML  ++ Q PV++Q+EVD LSK+RHPNI TLIG CPE   LVY+Y P G+LEDRL
Sbjct: 524 QVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITLIGACPEGWSLVYEYLPGGSLEDRL 583

Query: 547 ACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKLAGFG 606
            CKDNS PLSW+ R+ IAT++C+AL+F+HSNK    VHGD+KP+NILLD+N V KL+ FG
Sbjct: 584 TCKDNSPPLSWQNRVRIATEICAALVFLHSNKAHSLVHGDLKPANILLDSNLVSKLSDFG 643

Query: 607 TCHFLTHDEKLSYNENL--SVGY-DAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRMK 666
           TC  L  +   S   ++  +V Y D + +    LT + D+YSFG +LL LLTG+  LR+ 
Sbjct: 644 TCSLLHPNGSKSVRTDVTGTVAYLDPEASSSGELTPKSDVYSFGIILLRLLTGRPALRIS 703

Query: 667 EDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEPM 726
            +V+ A+    L D+LDP AGDWPFVQAEQLA+LALRCC+T S  RPDL ++VWRVLEPM
Sbjct: 704 NEVKYALDNGTLNDLLDPLAGDWPFVQAEQLARLALRCCETVSENRPDLGTEVWRVLEPM 763

Query: 727 RASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHD 786
           RAS GGS S  L   + ++  PPYFICPIFQEVM+DPHVAADGFTYEAEA+R WLDS HD
Sbjct: 764 RASSGGSSSFHLGRNEHRI-APPYFICPIFQEVMQDPHVAADGFTYEAEAIRAWLDSEHD 823

Query: 787 TSPMTNLRLDHQNLVPNRALRSVIQEWLQQH 813
           TSPMTN++L H +L+ N ALRS IQEWLQ H
Sbjct: 824 TSPMTNVKLSHTSLIANHALRSAIQEWLQHH 833

BLAST of CsaV3_6G000580 vs. TAIR 10
Match: AT3G49060.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 498.0 bits (1281), Expect = 1.4e-140
Identity = 298/811 (36.74%), Postives = 467/811 (57.58%), Query Frame = 0

Query: 7   EDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASSMKEEEV 66
           ++ ++VAV  D+   K  +++A ++  G++IC+L+VH  A+       K +  S K+ +V
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDV 73

Query: 67  NAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRSLVMGAA 126
                +E+   D++++ +L L  +   + +KL      I++ IVEL++ H I+ LVMGAA
Sbjct: 74  KVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAA 133

Query: 127 ADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADISPSSPQS 186
           +DK+YS KMT ++S+KA++V   A  SC I F+CKG LI  R +     +    P   Q 
Sbjct: 134 SDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIFTRASNDDSNNRQTMPPLVQL 193

Query: 187 QNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG----SPFERSYATSPSSG 246
            + N    ++E+            + +  RL    ++    G       ER      +  
Sbjct: 194 DSDNETR-KSEKL---------ESSYMRRRLRYWRSLLEQDGEKDTGQLEREKVEPRAPP 253

Query: 247 YMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIADA 306
                SS +  +  G    SP L+ +    TS  +    +++G     ++ + +KA+ D 
Sbjct: 254 LFSSGSSSSFGEPVGPEPVSPELVDSDTLNTS--NVEEKEREGDVARKVH-RYDKAMHDI 313

Query: 307 ANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDDVK 366
             + R  + EA K+ + +    EA+C AK  E L  +ES  RK  EE L KE+ E+  V 
Sbjct: 314 GQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELLEKEKLEVKMVI 373

Query: 367 NQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQRD 426
            Q N  MKELQ+ +   LKLE+Q+ +  ++ KE  +K  +A+ELL +++  RDE+    +
Sbjct: 374 EQNNGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMELLKSFRQKRDEIRIDHE 433

Query: 427 EALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGLLY 486
            A+KE++ +R        + S   + ++SF EI EAT +FDPS K+     GS+YKG L 
Sbjct: 434 NAVKEVNALRRLVKGETGESSGSEMLDYSFMEINEATNEFDPSWKLGEGKYGSVYKGNLQ 493

Query: 487 NTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNLED 546
           + +V++KML S+   N  +F+R V+ LS++RHPN+ TL+G CPE+  L+Y Y PNG+LED
Sbjct: 494 HLQVAVKMLPSYGSLNHFEFERRVEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLED 553

Query: 547 RLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKLAG 606
             + ++N   LSW++RI IA+++CSAL+F+HSN  C  +HG++KPS ILLD+N V K+  
Sbjct: 554 CFSSENNVPALSWESRIRIASEICSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKIND 613

Query: 607 FGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRMKE 666
           +G    +  D     + ++   Y   R     +T E DIY+FG +LL LLT +    +  
Sbjct: 614 YGISQLIPIDGLDKSDPHVDPHYFVSRE----MTLESDIYAFGIILLQLLTRRPVSGILR 673

Query: 667 DVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEPMR 726
           DV+ A++   +  VLD  AGDWP  + ++LA +A+RCC  + M RPDL + V R ++ M+
Sbjct: 674 DVKCALENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRPDL-AVVLRFIDRMK 733

Query: 727 ASCGGSLSVCLSFGDQQV--QPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH 786
           A    S S   S+ +Q V  +PP +++CPIFQEVM+DP +AADGFTYEAEA+R WL +GH
Sbjct: 734 APEVPS-SETSSYANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGH 793

Query: 787 DTSPMTNLRLDHQNLVPNRALRSVIQEWLQQ 812
           DTSPMTNL+++  NL+PN AL   IQ+W  Q
Sbjct: 794 DTSPMTNLKMEDCNLIPNHALHLAIQDWQNQ 804

BLAST of CsaV3_6G000580 vs. TAIR 10
Match: AT3G49060.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 475.3 bits (1222), Expect = 9.7e-134
Identity = 289/809 (35.72%), Postives = 457/809 (56.49%), Query Frame = 0

Query: 7   EDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASSMKEEEV 66
           ++ ++VAV  D+   K  +++A ++  G++IC+L+VH  A+       K +  S K+ +V
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDV 73

Query: 67  NAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRSLVMGAA 126
                +E+   D++++ +L L  +   + +KL      I++ IVEL++ H I+ LVMGAA
Sbjct: 74  KVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAA 133

Query: 127 ADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADISPSSPQS 186
           +DK+YS KMT ++S+KA++V   A  SC I F+CKG LI  R +     +    P   Q 
Sbjct: 134 SDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIFTRASNDDSNNRQTMPPLVQL 193

Query: 187 QNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNA--SGSPFERSYATSPSSGYM 246
            + N      +    +  R       ++E++ + +  ++        ER      +    
Sbjct: 194 DSDNETRKSEKLESSYMRRRLRYWRSLLEQVNLLQNCADGEKDTGQLEREKVEPRAPPLF 253

Query: 247 DVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIADAAN 306
              SS +  +  G    SP L+ +    TS  +    +++G     ++ + +KA+ D   
Sbjct: 254 SSGSSSSFGEPVGPEPVSPELVDSDTLNTS--NVEEKEREGDVARKVH-RYDKAMHDIGQ 313

Query: 307 ARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREELDDVKNQ 366
           + R  + EA K+ + +    EA+C AK  E L  +ES  RK  EE L KE+ E+  V  Q
Sbjct: 314 SDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELLEKEKLEVKMVIEQ 373

Query: 367 VNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQRDEA 426
            N  MKELQ+ +   LKLE+Q+ +  ++ KE  +K  +A+ELL +++  RDE+    + A
Sbjct: 374 NNGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMELLKSFRQKRDEIRIDHENA 433

Query: 427 LKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGLLYNT 486
           +KE++ +R        + S   + ++SF EI EAT +FDPS K+     GS+YKG L + 
Sbjct: 434 VKEVNALRRLVKGETGESSGSEMLDYSFMEINEATNEFDPSWKLGEGKYGSVYKGNLQHL 493

Query: 487 EVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRL 546
           +V+                  V+ LS++RHPN+ TL+G CPE+  L+Y Y PNG+LED  
Sbjct: 494 QVA------------------VEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCF 553

Query: 547 ACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKLAGFG 606
           + ++N   LSW++RI IA+++CSAL+F+HSN  C  +HG++KPS ILLD+N V K+  +G
Sbjct: 554 SSENNVPALSWESRIRIASEICSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKINDYG 613

Query: 607 TCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRMKEDV 666
               +  D     + ++   Y   R     +T E DIY+FG +LL LLT +    +  DV
Sbjct: 614 ISQLIPIDGLDKSDPHVDPHYFVSRE----MTLESDIYAFGIILLQLLTRRPVSGILRDV 673

Query: 667 QIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEPMRAS 726
           + A++   +  VLD  AGDWP  + ++LA +A+RCC  + M RPDL + V R ++ M+A 
Sbjct: 674 KCALENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRPDL-AVVLRFIDRMKAP 733

Query: 727 CGGSLSVCLSFGDQQV--QPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDT 786
              S S   S+ +Q V  +PP +++CPIFQEVM+DP +AADGFTYEAEA+R WL +GHDT
Sbjct: 734 EVPS-SETSSYANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGHDT 793

Query: 787 SPMTNLRLDHQNLVPNRALRSVIQEWLQQ 812
           SPMTNL+++  NL+PN AL   IQ+W  Q
Sbjct: 794 SPMTNLKMEDCNLIPNHALHLAIQDWQNQ 794

BLAST of CsaV3_6G000580 vs. TAIR 10
Match: AT4G25160.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 281.6 bits (719), Expect = 2.0e-75
Identity = 231/812 (28.45%), Postives = 381/812 (46.92%), Query Frame = 0

Query: 39  ILHVHEPAKFIPI-LGTKFLASSMKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEK 98
           +LH+H     +P  +G     S ++++ V AYR+     ++++L  +  L  +     E 
Sbjct: 53  LLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEV 112

Query: 99  LYFESDKIKKGIVELVSLHGIRSLVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQ 158
           L  ESD +   I E V+   I  +V+G ++  ++SRK        A+  +      C + 
Sbjct: 113 LVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSFFSRKADICSVISALMPNF-----CTVY 172

Query: 159 FVCKGQL-----------IRIREACPQETHADISPSSPQSQNINGVSWRTEQFGLFNGRI 218
            V KG+L             IRE   + T++    S P S + + +S   +         
Sbjct: 173 VVSKGKLSCVRPSDSDGNATIREDGSERTNSSSGSSGPTSDSSDVMSSAHDS-------Q 232

Query: 219 SNSPTIVMERLTISETISNASGSPFERSYATSPSSGYMDVASSRTEEDAYGLGLNSPLLL 278
           S   ++ + R+     I+  +  P E S   S  +  M +  +    D   +  +S    
Sbjct: 233 SRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSL-DAEEARDVSSINRSS---- 292

Query: 279 TNFATKTSPPHFSGFQQDGSADDSLYIQLEKAIADAANARREA-FREALKRAKAEKELDE 338
           T+  ++ +P      ++D       Y + ++A++ +++ R    F      +    +   
Sbjct: 293 TDTTSRWTPR-----RRD-------YEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTH 352

Query: 339 AICWAKVSETLYAEESRGRKDAEEELSKEREELDDVKNQVNEMMKELQ---------IAR 398
           +    + S    A   +   D +  L+ E E+L      V EM    Q         +  
Sbjct: 353 SRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGE 412

Query: 399 NNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRD----------ELLKQRDEALK 458
            N  +LE  I   +  +KE E + L+  E  +  K  RD          E+ ++R+   K
Sbjct: 413 LNQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAERK 472

Query: 459 ELDDIRTRQVEAMSQHSAQL-ISEFSFSEIVEATRKFDPSLKIVTDANGSMYKGLLYNTE 518
              D + ++    +  S QL    F++ EI+ AT  F   LKI   A G++YK  L++T 
Sbjct: 473 SARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTT 532

Query: 519 VSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRLA 578
             +K+L S   Q    FQ+E++ LSK+RHP++  L+G CPE   LVY+Y  NG+LEDRL 
Sbjct: 533 AVVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLF 592

Query: 579 CKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVPKLAGFGT 638
             +NS PL W  R  IA ++ +AL+F+H +K    +H D+KP+NILLD N+V K+   G 
Sbjct: 593 QVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGL 652

Query: 639 CHFLTHDEKLS----YNENLSVG----YDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSY 698
              +  D   +    Y +   VG     D +      ++++ DIYSFG +LL LLT K  
Sbjct: 653 STMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPA 712

Query: 699 LRMKEDVQIAIKKR-KLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWR 758
           + +   V+ A+    +   +LD +AG+WP  +  +LA LAL C +     RPDL   +  
Sbjct: 713 IALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILP 772

Query: 759 VLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWL 809
            LE ++     + +   SF     QPP +FICP+ ++VM +P VAADG+TY+  A+  WL
Sbjct: 773 ALENLKKVAEKARN---SFSGVSTQPPTHFICPLLKDVMNEPCVAADGYTYDRHAIEEWL 831

BLAST of CsaV3_6G000580 vs. TAIR 10
Match: AT5G61550.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 272.3 bits (695), Expect = 1.2e-72
Identity = 226/819 (27.59%), Postives = 381/819 (46.52%), Query Frame = 0

Query: 39  ILHVHEPAKFIPI-LGTKFLASSMKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEK 98
           +L+V  P  +IP  +G     S ++E+ V+AY++     A+++L  +  +  +   + E 
Sbjct: 55  LLYVRPPVSYIPTPMGIAVAVSELREDVVSAYKQELDWSANEMLRPYKKMFERRKVQVEV 114

Query: 99  LYFESDKIKKGIVELVSLHGIRSLVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQ 158
           L  +S +    I E ++  G+  LV+G +   ++SRK+       +  +       C + 
Sbjct: 115 LLLDSLEPAAAIAEEIAGTGVTKLVIGMSLRGFFSRKID-----MSSLIATAVPRFCTVY 174

Query: 159 FVCKGQLIRIREACPQETHADISPSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERL 218
            + KG+L  +R   P E+ A  S    +S + +G               ++SP +  E  
Sbjct: 175 VISKGKLASVR---PSESDASGSIRFERSSSTSG--------------STDSPRLPPEYQ 234

Query: 219 TISETISNASG--SPFERSYATSPSS---GYMDVASSRTEEDAYGLGLNSPLLLTNFATK 278
                +S A    SPF  +   S  S     MD +SS T+++    G    ++ +    K
Sbjct: 235 DFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGTDQEEVSTGRGMEIVHSGIEGK 294

Query: 279 TSPPHFSGFQQDGSADDSLYIQLEKAIADAANARREAFREALKRAKAEKELD-------- 338
            +        +  SA   +  +   +++  +  R    R  ++ + +    D        
Sbjct: 295 KNK------DESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSSSSSNNHDLVNMDWGA 354

Query: 339 ---EAICW-----AKVSETLYAEES--RGRKDAEEELSKEREELDDVKNQVNEMMKELQI 398
              E   W     + +S+ L +  S    + +   E+ K R EL  V+        E   
Sbjct: 355 VVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELKHVQEMYAMAQTETVG 414

Query: 399 ARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELLKQRDEALKEL---DDI 458
           A     +L  +  +  E + EL++K   A +     K   +E +K+  E +KEL   + +
Sbjct: 415 ASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMKEA-EKVKELMMKEAL 474

Query: 459 RTRQVEAMSQHSAQ--------LIS------EFSFSEIVEATRKFDPSLKIVTDANGSMY 518
             R+ E  ++  A+        L+S       +++ EI  AT  F  +LKI   A GS+Y
Sbjct: 475 HRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFAENLKIGIGAYGSVY 534

Query: 519 KGLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPN 578
           K  L++T  ++K+L +   Q    F +E++ LSK+RHP++  L+G CPE   LVY+Y  N
Sbjct: 535 KCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGACPERGCLVYEYMDN 594

Query: 579 GNLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYV 638
           G+L+DRL   +++ P+ W  R  IA ++ SAL+F+H +K    +H D+KP NILLD N+V
Sbjct: 595 GSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHRDLKPGNILLDHNFV 654

Query: 639 PKLAGFGTCHFLTHDEKLS---YNENLSVG----YDAKRNHEFPLTTELDIYSFGTVLLS 698
            KL   G    +  D+  S   + +   VG     D +      ++ + D+YS G V+L 
Sbjct: 655 SKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQRTGIISPKSDVYSLGVVILQ 714

Query: 699 LLTGKSYLRMKEDVQIAI-KKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPD 758
           L+T K  + +   V+ AI    +   +LD +AG WP     +LA L L C +     RPD
Sbjct: 715 LITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTRELAALGLCCTEMRRRDRPD 774

Query: 759 LVSDVWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEA 809
           L   +   LE +R     + ++          PP +FICP+ + VM +P VAADG+TY+ 
Sbjct: 775 LKDQIIPALERLRKVADKAQNL---LSRTPSGPPSHFICPLLKGVMNEPCVAADGYTYDR 834