CsaV3_5G030250 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_5G030250 vs. NCBI nr
Match: XP_004135327.1 (alpha-ketoglutarate-dependent dioxygenase alkB isoform X1 [Cucumis sativus])

HSP 1 Score: 524.2 bits (1349), Expect = 6.1e-145
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0

Query: 1   MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK 60
           MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK
Sbjct: 109 MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK 168

Query: 61  FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 120
           FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA
Sbjct: 169 FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 228

Query: 121 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG 180
           KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG
Sbjct: 229 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG 288

Query: 181 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE 240
           KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE
Sbjct: 289 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE 348

Query: 241 YIRTSRININIRQVF 256
           YIRTSRININIRQVF
Sbjct: 349 YIRTSRININIRQVF 363

BLAST of CsaV3_5G030250 vs. NCBI nr
Match: XP_031740902.1 (alpha-ketoglutarate-dependent dioxygenase alkB isoform X2 [Cucumis sativus])

HSP 1 Score: 524.2 bits (1349), Expect = 6.1e-145
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0

Query: 1   MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK 60
           MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK
Sbjct: 106 MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK 165

Query: 61  FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 120
           FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA
Sbjct: 166 FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 225

Query: 121 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG 180
           KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG
Sbjct: 226 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG 285

Query: 181 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE 240
           KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE
Sbjct: 286 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE 345

Query: 241 YIRTSRININIRQVF 256
           YIRTSRININIRQVF
Sbjct: 346 YIRTSRININIRQVF 360

BLAST of CsaV3_5G030250 vs. NCBI nr
Match: KAE8648677.1 (hypothetical protein Csa_009164 [Cucumis sativus])

HSP 1 Score: 524.2 bits (1349), Expect = 6.1e-145
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0

Query: 1   MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK 60
           MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK
Sbjct: 1   MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK 60

Query: 61  FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 120
           FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA
Sbjct: 61  FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 120

Query: 121 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG 180
           KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG
Sbjct: 121 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG 180

Query: 181 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE 240
           KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE
Sbjct: 181 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE 240

Query: 241 YIRTSRININIRQVF 256
           YIRTSRININIRQVF
Sbjct: 241 YIRTSRININIRQVF 255

BLAST of CsaV3_5G030250 vs. NCBI nr
Match: XP_008446031.1 (PREDICTED: LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB [Cucumis melo])

HSP 1 Score: 500.4 bits (1287), Expect = 9.5e-138
Identity = 245/255 (96.08%), Postives = 248/255 (97.25%), Query Frame = 0

Query: 1   MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK 60
           M+FPQPPNRTNHNAIYGPIQDLFIAAKE KVLVEHDEISDF LDSDVEPSISNG+THNWK
Sbjct: 109 MDFPQPPNRTNHNAIYGPIQDLFIAAKEKKVLVEHDEISDFNLDSDVEPSISNGSTHNWK 168

Query: 61  FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 120
           FVEENTVSSRRGTA KSI ASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA
Sbjct: 169 FVEENTVSSRRGTACKSISASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 228

Query: 121 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG 180
           KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKA FLLGG
Sbjct: 229 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAXFLLGG 288

Query: 181 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE 240
           KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLE HL+NQDDLH LE
Sbjct: 289 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLERHLSNQDDLHFLE 348

Query: 241 YIRTSRININIRQVF 256
           YIRTSRININIRQVF
Sbjct: 349 YIRTSRININIRQVF 363

BLAST of CsaV3_5G030250 vs. NCBI nr
Match: XP_038891364.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Benincasa hispida])

HSP 1 Score: 476.9 bits (1226), Expect = 1.1e-130
Identity = 234/253 (92.49%), Postives = 240/253 (94.86%), Query Frame = 0

Query: 3   FPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFV 62
           FPQP NRTNHNAIYG IQDLFIAAK  KVLVE DEISDF +DSDVE S+SNGNTHNWKFV
Sbjct: 111 FPQPSNRTNHNAIYGSIQDLFIAAKGKKVLVEDDEISDFNVDSDVERSVSNGNTHNWKFV 170

Query: 63  EENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKR 122
           EENTVSS+RGTA KSIPASVLLRKLRWSTLGLQFDWSKRSY+ISLPHNKIPSALCQLAKR
Sbjct: 171 EENTVSSKRGTACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKR 230

Query: 123 MAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS 182
           MAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS
Sbjct: 231 MAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS 290

Query: 183 RQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYI 242
           RQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEIS LE H +NQDDLH LEYI
Sbjct: 291 RQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISLLERHFSNQDDLHFLEYI 350

Query: 243 RTSRININIRQVF 256
           + SRININIRQVF
Sbjct: 351 KNSRININIRQVF 363

BLAST of CsaV3_5G030250 vs. ExPASy Swiss-Prot
Match: Q9SA98 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 GN=At1g11780 PE=2 SV=2)

HSP 1 Score: 355.5 bits (911), Expect = 4.9e-97
Identity = 176/253 (69.57%), Postives = 199/253 (78.66%), Query Frame = 0

Query: 3   FPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFV 62
           FPQPPNRTNHNAIYGPI DLF +AKENKVLV+ D              ++N   + WKF 
Sbjct: 110 FPQPPNRTNHNAIYGPIDDLFDSAKENKVLVQDD--------------LTN---NKWKFY 169

Query: 63  EENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKR 122
           EE  +     ++ KS+ ASVLLRKLRWSTLGLQFDWSKR+Y++SLPHN IP ALCQLAK 
Sbjct: 170 EEVDIEKATRSSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKT 229

Query: 123 MAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS 182
            AA AMP GEEF+PE AIVNYF  GDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS
Sbjct: 230 HAAIAMPDGEEFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS 289

Query: 183 RQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYI 242
           + DPP AM+LRSGDVVLMAGEARECFHG+PRIF  EE+ +I  LE+ L+++      EYI
Sbjct: 290 KDDPPHAMYLRSGDVVLMAGEARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYI 345

Query: 243 RTSRININIRQVF 256
           +TSRININIRQVF
Sbjct: 350 KTSRININIRQVF 345

BLAST of CsaV3_5G030250 vs. ExPASy Swiss-Prot
Match: Q54N08 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44689 GN=alkB PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 5.0e-33
Identity = 84/259 (32.43%), Postives = 142/259 (54.83%), Query Frame = 0

Query: 2   EFPQPPNRTNHNAIYGPIQDLFIAAKE---NKVLVEHDEISDFKLDSDVEPSISNGNTHN 61
           ++  PPN  N    +GPI++L+   ++   N+ L    +  D +++    P   NG    
Sbjct: 147 DYADPPNNNNITLFHGPIKNLWKNGEKELINEELKSQGKHDDDEIEQPTRPLDKNG---- 206

Query: 62  WKFVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQ 121
                E   + R+           LL KL WSTLG Q+ W+ R Y+    + + P  L +
Sbjct: 207 -----EPLPTYRQ-----------LLDKLAWSTLGYQYQWTPRLYSEEF-YEEFPDDLQE 266

Query: 122 LAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLL 181
           L +++A A     + +  EAA VN+++    +GGHLDD E +  KPI+S+S G  A+FL+
Sbjct: 267 LVQKIAIAT--KFDPYVAEAATVNFYSEDSIMGGHLDDAEQEMEKPIISISFGSTAVFLM 326

Query: 182 GGKSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHC 241
           G ++R   P+ +F+RSGD+V+M G +R C+HGV +I   E S ++  ++ +  +QD  + 
Sbjct: 327 GAETRDIAPVPLFIRSGDIVIMGGRSRYCYHGVAKIV--ENSFDLGLIDEN-DDQDLKYK 379

Query: 242 LEYI--RTSRININIRQVF 256
           ++++  +  R+NIN RQVF
Sbjct: 387 IQWLKEKNRRVNINTRQVF 379

BLAST of CsaV3_5G030250 vs. ExPASy Swiss-Prot
Match: Q13686 (Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-29
Identity = 83/226 (36.73%), Postives = 124/226 (54.87%), Query Frame = 0

Query: 48  EPSISNGNTHNWK-----FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRS 107
           +P++ N + H  K       E++    R   A K  P S LL KLRW T+G  ++W  + 
Sbjct: 125 KPNVCNLDKHMSKEETQDLWEQSKEFLRYKEATKRRPRS-LLEKLRWVTVGYHYNWDSKK 184

Query: 108 YNISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWS 167
           Y+    +   PS L  L++++AAA     E+F+ EA I+NY+    TLG H+D  E D S
Sbjct: 185 YSAD-HYTPFPSDLGFLSEQVAAAC--GFEDFRAEAGILNYYRLDSTLGIHVDRSELDHS 244

Query: 168 KPIVSMSLGCKAIFLLGGKSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEE 227
           KP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H VPR+  + E E 
Sbjct: 245 KPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNHAVPRVLPNPEGEG 304

Query: 228 ISF-LETHL-------------TNQDDLHCLEYIRTSRININIRQV 255
           +   LE  L             + +D   C  Y++T+R+N+ +RQV
Sbjct: 305 LPHCLEAPLPAVLPRDSMVEPCSMEDWQVCASYLKTARVNMTVRQV 346

BLAST of CsaV3_5G030250 vs. ExPASy Swiss-Prot
Match: P0CB42 (Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.2e-28
Identity = 82/226 (36.28%), Postives = 121/226 (53.54%), Query Frame = 0

Query: 48  EPSISNGNTHNWK-----FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRS 107
           +P++ N + H  K       E++    R     K  P S LL +LRW TLG  ++W  + 
Sbjct: 125 KPNVCNLDKHMTKEETQGLWEQSKEVLRSKEVTKRRPRS-LLERLRWVTLGYHYNWDSKK 184

Query: 108 YNISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWS 167
           Y+    +   PS L  L++++A A    G  F+ EA I+NY+    TLG H+D  E D S
Sbjct: 185 YSAD-HYTPFPSDLAFLSEQVATACGFQG--FQAEAGILNYYRLDSTLGIHVDRSELDHS 244

Query: 168 KPIVSMSLGCKAIFLLGGKSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEE 227
           KP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H VPR+    + E 
Sbjct: 245 KPLLSFSFGQSAIFLLGGLKRDEAPTAMFMHSGDIMVMSGFSRLLNHAVPRVLPHPDGEC 304

Query: 228 ISF-LETHL-------------TNQDDLHCLEYIRTSRININIRQV 255
           +   LET L             + +D   C  Y+RT+R+N+ +RQV
Sbjct: 305 LPHCLETPLPAVLPSNSLVEPCSVEDWQVCATYLRTARVNMTVRQV 346

BLAST of CsaV3_5G030250 vs. ExPASy Swiss-Prot
Match: O60066 (Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=abh1 PE=2 SV=3)

HSP 1 Score: 118.6 bits (296), Expect = 1.0e-25
Identity = 63/170 (37.06%), Postives = 97/170 (57.06%), Query Frame = 0

Query: 85  RKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYF 144
           +KLRW TLG Q+DW+ + Y         P  L    +++   +      +K EAAIVN++
Sbjct: 138 KKLRWVTLGEQYDWTTKEYPDPSKSPGFPKDLGDFVEKVVKESTDF-LHWKAEAAIVNFY 197

Query: 145 ASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFLRSGDVVLMAGEA 204
           + GDTL  H+D+ E D + P++S+S+G   I+L+G +SR + P A+ L SGDVV+M G +
Sbjct: 198 SPGDTLSAHIDESEEDLTLPLISLSMGLDCIYLIGTESRSEKPSALRLHSGDVVIMTGTS 257

Query: 205 RECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININIRQV 255
           R+ FH VP+I     +   ++L T     D      +I   R+N N+RQV
Sbjct: 258 RKAFHAVPKII---PNSTPNYLLTGNKAWDG-----WISRKRVNFNVRQV 298

BLAST of CsaV3_5G030250 vs. ExPASy TrEMBL
Match: A0A0A0KS00 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589370 PE=4 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.0e-145
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0

Query: 1   MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK 60
           MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK
Sbjct: 109 MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK 168

Query: 61  FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 120
           FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA
Sbjct: 169 FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 228

Query: 121 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG 180
           KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG
Sbjct: 229 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG 288

Query: 181 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE 240
           KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE
Sbjct: 289 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE 348

Query: 241 YIRTSRININIRQVF 256
           YIRTSRININIRQVF
Sbjct: 349 YIRTSRININIRQVF 363

BLAST of CsaV3_5G030250 vs. ExPASy TrEMBL
Match: A0A1S3BE32 (LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis melo OX=3656 GN=LOC103488884 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 4.6e-138
Identity = 245/255 (96.08%), Postives = 248/255 (97.25%), Query Frame = 0

Query: 1   MEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWK 60
           M+FPQPPNRTNHNAIYGPIQDLFIAAKE KVLVEHDEISDF LDSDVEPSISNG+THNWK
Sbjct: 109 MDFPQPPNRTNHNAIYGPIQDLFIAAKEKKVLVEHDEISDFNLDSDVEPSISNGSTHNWK 168

Query: 61  FVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 120
           FVEENTVSSRRGTA KSI ASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA
Sbjct: 169 FVEENTVSSRRGTACKSISASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLA 228

Query: 121 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGG 180
           KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKA FLLGG
Sbjct: 229 KRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAXFLLGG 288

Query: 181 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLE 240
           KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLE HL+NQDDLH LE
Sbjct: 289 KSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLERHLSNQDDLHFLE 348

Query: 241 YIRTSRININIRQVF 256
           YIRTSRININIRQVF
Sbjct: 349 YIRTSRININIRQVF 363

BLAST of CsaV3_5G030250 vs. ExPASy TrEMBL
Match: A0A6J1FZQ9 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=LOC111449397 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.2e-124
Identity = 222/254 (87.40%), Postives = 236/254 (92.91%), Query Frame = 0

Query: 2   EFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKF 61
           +FPQPPNRTNHNAIYGPIQDLFIAAKE +VLVE +EIS   +DSD EPS+ NGN++ WKF
Sbjct: 110 DFPQPPNRTNHNAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKF 169

Query: 62  VEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAK 121
           VEENTVSSRRGT  KS+PAS LLRKLRWSTLGLQFDWSKRSY+ISLPHN+IPSALCQL K
Sbjct: 170 VEENTVSSRRGTC-KSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTK 229

Query: 122 RMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGK 181
           RMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGK
Sbjct: 230 RMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGK 289

Query: 182 SRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEY 241
           SRQDPP+AMFLRSGDVVLMAGEARECFHGVPRIF DEESEEIS LE   +++DDLH LEY
Sbjct: 290 SRQDPPVAMFLRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEY 349

Query: 242 IRTSRININIRQVF 256
           IRTSRININIRQVF
Sbjct: 350 IRTSRININIRQVF 362

BLAST of CsaV3_5G030250 vs. ExPASy TrEMBL
Match: A0A6J1DG90 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN=LOC111019811 PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 3.8e-124
Identity = 222/253 (87.75%), Postives = 235/253 (92.89%), Query Frame = 0

Query: 3   FPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFV 62
           FPQPPNRTNHNAIYGPIQDLFIAAKE +VLVE +EIS F +DSD+EPS+S GNTH WKFV
Sbjct: 111 FPQPPNRTNHNAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFV 170

Query: 63  EENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKR 122
           EENTVSSRR T  KS+PASVLLRKLRWSTLGLQFDWSKRSY+ISL HNK+PSALCQLAKR
Sbjct: 171 EENTVSSRR-TTCKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKR 230

Query: 123 MAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS 182
           MAAAAMP GEEFKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS
Sbjct: 231 MAAAAMPAGEEFKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS 290

Query: 183 RQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYI 242
           RQDPP+AMFLRSGDVVLMAGEARECFHGVPRIFID E++E S LE   +NQDDLH LEYI
Sbjct: 291 RQDPPVAMFLRSGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYI 350

Query: 243 RTSRININIRQVF 256
           RTSRININIRQVF
Sbjct: 351 RTSRININIRQVF 362

BLAST of CsaV3_5G030250 vs. ExPASy TrEMBL
Match: A0A6J1HQB4 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LOC111466401 PE=4 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 3.2e-123
Identity = 221/254 (87.01%), Postives = 235/254 (92.52%), Query Frame = 0

Query: 2   EFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKF 61
           +FPQPPNRTNHNAIYGPIQDLFIAAKE +VLVE +EIS   +DSD EPS+ NGN++ WKF
Sbjct: 110 DFPQPPNRTNHNAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKF 169

Query: 62  VEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAK 121
           VEENTVSSRRGT  KS+PAS LLRKLRWSTLGLQFDWSKRSY+ISLPHN IPSALCQLAK
Sbjct: 170 VEENTVSSRRGTC-KSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNDIPSALCQLAK 229

Query: 122 RMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGK 181
           RMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGK
Sbjct: 230 RMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGK 289

Query: 182 SRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEY 241
           SRQD P+AMFLRSGDVVLMAGEARECFHGVPRIF DEESEEIS LE   +++DDLH LEY
Sbjct: 290 SRQDLPVAMFLRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEY 349

Query: 242 IRTSRININIRQVF 256
           +RTSRININIRQVF
Sbjct: 350 MRTSRININIRQVF 362

BLAST of CsaV3_5G030250 vs. TAIR 10
Match: AT1G11780.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 355.5 bits (911), Expect = 3.5e-98
Identity = 176/253 (69.57%), Postives = 199/253 (78.66%), Query Frame = 0

Query: 3   FPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFV 62
           FPQPPNRTNHNAIYGPI DLF +AKENKVLV+ D              ++N   + WKF 
Sbjct: 110 FPQPPNRTNHNAIYGPIDDLFDSAKENKVLVQDD--------------LTN---NKWKFY 169

Query: 63  EENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKR 122
           EE  +     ++ KS+ ASVLLRKLRWSTLGLQFDWSKR+Y++SLPHN IP ALCQLAK 
Sbjct: 170 EEVDIEKATRSSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKT 229

Query: 123 MAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS 182
            AA AMP GEEF+PE AIVNYF  GDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS
Sbjct: 230 HAAIAMPDGEEFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS 289

Query: 183 RQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYI 242
           + DPP AM+LRSGDVVLMAGEARECFHG+PRIF  EE+ +I  LE+ L+++      EYI
Sbjct: 290 KDDPPHAMYLRSGDVVLMAGEARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYI 345

Query: 243 RTSRININIRQVF 256
           +TSRININIRQVF
Sbjct: 350 KTSRININIRQVF 345

BLAST of CsaV3_5G030250 vs. TAIR 10
Match: AT3G14160.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 65.5 bits (158), Expect = 7.3e-11
Identity = 43/122 (35.25%), Postives = 61/122 (50.00%), Query Frame = 0

Query: 136 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPIAMF 195
           P+  IVN+++S   LG H D  E++ S     P+VS S+G  A FL G +  +D    + 
Sbjct: 346 PDICIVNFYSSTGRLGLHQDKDESENSIRKGLPVVSFSIGDSAEFLYGDQRDEDKAETLT 405

Query: 196 LRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININI 254
           L SGDV+L  G +R+ FHGV  I  D   + +   ET L            R  R+N+  
Sbjct: 406 LESGDVLLFGGRSRKVFHGVRSIRKDTAPKAL-LQETSL------------RPGRLNLTF 454

BLAST of CsaV3_5G030250 vs. TAIR 10
Match: AT5G01780.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.8e-10
Identity = 44/121 (36.36%), Postives = 60/121 (49.59%), Query Frame = 0

Query: 136 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPIAMF 195
           P+  IVN+++    LG H D  E++ S     PIVS S+G  A FL G K   +    + 
Sbjct: 278 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 337

Query: 196 LRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININI 253
           L SGDV++  GE+R  FHGV  I     S  +S L     N+  L      RT R+N+  
Sbjct: 338 LESGDVLIFGGESRMIFHGVKSII--PNSAPMSLL-----NESKL------RTGRLNLTF 385

BLAST of CsaV3_5G030250 vs. TAIR 10
Match: AT5G01780.2 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.8e-10
Identity = 44/121 (36.36%), Postives = 60/121 (49.59%), Query Frame = 0

Query: 136 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPIAMF 195
           P+  IVN+++    LG H D  E++ S     PIVS S+G  A FL G K   +    + 
Sbjct: 333 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 392

Query: 196 LRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININI 253
           L SGDV++  GE+R  FHGV  I     S  +S L     N+  L      RT R+N+  
Sbjct: 393 LESGDVLIFGGESRMIFHGVKSII--PNSAPMSLL-----NESKL------RTGRLNLTF 440

BLAST of CsaV3_5G030250 vs. TAIR 10
Match: AT3G14140.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 48.9 bits (115), Expect = 7.0e-06
Identity = 34/117 (29.06%), Postives = 48/117 (41.03%), Query Frame = 0

Query: 136 PEAAIVNYFASGDTLGGH----LDDMEADWSK---------------------PIVSMSL 195
           P+  +VN++ S   LG H     D    D+ K                     PIVS S+
Sbjct: 351 PDICVVNFYTSTGKLGLHQVSVYDKTSFDFLKYKGGYLNTDKGESKKSLRKGLPIVSFSI 410

Query: 196 GCKAIFLLGGKSRQDPPIAMFLRSGDVVLMAGEARECFHGV--------PRIFIDEE 220
           G  A FL G +   D    + L SGDV++    +R  FHGV        PR+F  ++
Sbjct: 411 GDSAEFLYGDQKDVDKADTLILESGDVLIFGERSRNVFHGVRSIRKILPPRLFFRKQ 467