CsaV3_5G014390 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_5G014390 vs. NCBI nr
Match: XP_004142262.1 (sec14 cytosolic factor isoform X1 [Cucumis sativus] >KGN49599.1 hypothetical protein Csa_018340 [Cucumis sativus])

HSP 1 Score: 484.6 bits (1246), Expect = 5.1e-133
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV
Sbjct: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60

Query: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120
           PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI
Sbjct: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180
           ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240
           VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH
Sbjct: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240

Query: 241 LHG 244
           LHG
Sbjct: 241 LHG 243

BLAST of CsaV3_5G014390 vs. NCBI nr
Match: XP_008452419.1 (PREDICTED: sec14 cytosolic factor-like [Cucumis melo])

HSP 1 Score: 449.1 bits (1154), Expect = 2.4e-122
Identity = 222/242 (91.74%), Postives = 233/242 (96.28%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           ME S+ANAV  LR+SI KSGFSTQDYSDATMMRFLIARSMEV KAAKMFVQWKKWRDATV
Sbjct: 1   MEDSNANAVTLLRNSIEKSGFSTQDYSDATMMRFLIARSMEVSKAAKMFVQWKKWRDATV 60

Query: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120
           PKG IAESEVEDELKAKKIFLQG+SIKQLPVMIV+ANRHFHSKDQ+QFKKF+VH+LDKVI
Sbjct: 61  PKGYIAESEVEDELKAKKIFLQGMSIKQLPVMIVIANRHFHSKDQLQFKKFVVHILDKVI 120

Query: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180
           ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240
           VSIWRM+SRFVDTATLKNILIVS+EEEKR MIEEVGE+VLPIEYGGKAKF  LQDVV+PH
Sbjct: 181 VSIWRMVSRFVDTATLKNILIVSNEEEKRNMIEEVGEDVLPIEYGGKAKFFALQDVVVPH 240

Query: 241 LH 243
           LH
Sbjct: 241 LH 242

BLAST of CsaV3_5G014390 vs. NCBI nr
Match: XP_038893024.1 (sec14 cytosolic factor-like [Benincasa hispida])

HSP 1 Score: 431.4 bits (1108), Expect = 5.2e-117
Identity = 209/242 (86.36%), Postives = 229/242 (94.63%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           ME S  NA+IQLR+S+ KSGFSTQ+ SDATMMRFLIARSM+V KA+KMFVQW+KWRDA V
Sbjct: 1   MEDSGTNAMIQLRNSVEKSGFSTQNCSDATMMRFLIARSMDVSKASKMFVQWRKWRDAMV 60

Query: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120
           PKG IAESEVEDELKAKKIFLQG+SI QLPVMIV+ANRHFH+KDQ+QFKKF+VHLLDKVI
Sbjct: 61  PKGFIAESEVEDELKAKKIFLQGLSISQLPVMIVLANRHFHAKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180
           ASGCKGKEIGNEKWIAIVDL+QISYKN+DPRGLIT FQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGKEIGNEKWIAIVDLRQISYKNIDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240
           VS+WRM+SRFVD+ATLKNI IVS+EEE+RIMIEEVGEEVLP EYGGKAKFI LQDV+LPH
Sbjct: 181 VSVWRMVSRFVDSATLKNISIVSNEEERRIMIEEVGEEVLPDEYGGKAKFIALQDVLLPH 240

Query: 241 LH 243
           LH
Sbjct: 241 LH 242

BLAST of CsaV3_5G014390 vs. NCBI nr
Match: XP_022941638.1 (SEC14 cytosolic factor-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 397.5 bits (1020), Expect = 8.2e-107
Identity = 198/241 (82.16%), Postives = 215/241 (89.21%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           ME S ANA+IQ+R ++ KSG +TQ+Y DATMMRFLIARSME  K AKMFVQW+KWRDA V
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120
           PKG I+ESEV++EL+AKKIFLQG+S   L VMIVMA RHF SKDQ+QFKKF+VHLLDKVI
Sbjct: 61  PKGFISESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180
           ASG KGKEIGNEKWIAIVDL QISYKNVD RGLIT FQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240
           VSIWRM+SRFVD ATLKNILIVS+EEE+R MIEEVGEEVLP EYGGKAKFI LQD VLPH
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 L 242
           L
Sbjct: 241 L 241

BLAST of CsaV3_5G014390 vs. NCBI nr
Match: KAG6599921.1 (hypothetical protein SDJN03_05154, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 397.5 bits (1020), Expect = 8.2e-107
Identity = 198/241 (82.16%), Postives = 214/241 (88.80%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           ME S ANA+IQ+R ++ KSG +TQ+Y DATMMRFLIARSME  K AKMFVQW+KWRDA V
Sbjct: 1   MENSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120
           PKG I ESEV++EL+AKKIFLQG+S   L VMIVMA RHF SKDQ+QFKKF+VHLLDKVI
Sbjct: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180
           ASG KGKEIGNEKWIAIVDL QISYKNVD RGLIT FQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240
           VSIWRM+SRFVD ATLKNILIVS+EEE+R MIEEVGEEVLP EYGGKAKFI LQD VLPH
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 L 242
           L
Sbjct: 241 L 241

BLAST of CsaV3_5G014390 vs. ExPASy Swiss-Prot
Match: Q10137 (Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sec14 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.9e-13
Identity = 63/242 (26.03%), Postives = 110/242 (45.45%), Query Frame = 0

Query: 12  LRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATVPKGLIAESEVE 71
           +R  + K G+ T+   DAT++RFL AR   + ++ +MF++ +KWR       LI     +
Sbjct: 35  MRLELQKLGY-TERLDDATLLRFLRARKFNLQQSLEMFIKCEKWRKEFGVDDLIKNFHYD 94

Query: 72  DELKAKKIF------------------LQGISIKQLPVMIV---MANRHFHSKDQVQFKK 131
           ++    K +                  L  I +K+L  +     M     +  + +  K+
Sbjct: 95  EKEAVSKYYPQFYHKTDIDGRPVYVEQLGNIDLKKLYQITTPERMMQNLVYEYEMLALKR 154

Query: 132 FIVHLLDKVIASGCKGKEIG-NEKWIAIVDLQQISYKNVDP--RGLITAFQFLQNYYPER 191
           F            C  K  G  E    I+DL+ +   ++      +  A    Q+YYPER
Sbjct: 155 F----------PACSRKAGGLIETSCTIMDLKGVGITSIHSVYSYIRQASSISQDYYPER 214

Query: 192 LGKCFILNMPWFFVSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGK 230
           +GK +++N PW F S + +I  F+D AT+K I I+ S   K  ++E++  + LP + GG 
Sbjct: 215 MGKFYVINAPWGFSSAFNLIKGFLDEATVKKIHILGS-NYKSALLEQIPADNLPAKLGGN 264

BLAST of CsaV3_5G014390 vs. ExPASy Swiss-Prot
Match: P24280 (SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEC14 PE=1 SV=3)

HSP 1 Score: 77.8 bits (190), Expect = 1.9e-13
Identity = 66/247 (26.72%), Postives = 123/247 (49.80%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWR---- 60
           ++ +   A+ +LR  +  +GF  +   D+T++RFL AR  +V  A +MF   +KWR    
Sbjct: 29  LDSAQEKALAELRKLLEDAGF-IERLDDSTLLRFLRARKFDVQLAKEMFENCEKWRKDYG 88

Query: 61  -DATV------PKGLIAESEVEDELKAKK----IFLQGISIKQLPVMIVMANRHFHSKDQ 120
            D  +       K LIA+   +   K  K    ++ + +    L  M  + +     K+ 
Sbjct: 89  TDTILQDFHYDEKPLIAKFYPQYYHKTDKDGRPVYFEELGAVNLHEMNKVTSEERMLKNL 148

Query: 121 V-QFKKFIVHLLDKVIASGCKGKEIGNEKWIAIVDLQQISYKNV-DPRGLITAFQFL-QN 180
           V +++  + + L     S   G  +  E    I+DL+ IS  +       +    ++ QN
Sbjct: 149 VWEYESVVQYRLP--ACSRAAGHLV--ETSCTIMDLKGISISSAYSVMSYVREASYISQN 208

Query: 181 YYPERLGKCFILNMPWFFVSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPI 230
           YYPER+GK +I+N P+ F + +R+   F+D  T+  I I+ S  +K  +++++  E LP+
Sbjct: 209 YYPERMGKFYIINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQKE-LLKQIPAENLPV 268

BLAST of CsaV3_5G014390 vs. ExPASy Swiss-Prot
Match: Q75DK1 (SEC14 cytosolic factor OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 1.2e-12
Identity = 58/239 (24.27%), Postives = 115/239 (48.12%), Query Frame = 0

Query: 8   AVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATVPKGLIAE 67
           A+ +LR  + ++GF T+   D+T++RFL AR  +V  A  MF   +KWR       +  +
Sbjct: 34  ALEELRKVLKQAGF-TKRLDDSTLLRFLRARKFDVAAARAMFENCEKWRKENGVDTIFED 93

Query: 68  SEVED---------------ELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQV-QFKKF 127
              E+               +   + ++++ +    L  M  +  +    K+ + +++ F
Sbjct: 94  FHYEEKPLVAKFYPQYYHKTDKDGRPVYIEELGAVNLTEMYKITTQERMLKNLIWEYESF 153

Query: 128 IVHLLDKVIASGCKGKEIGNEKWIAIVDLQQISYKNVDP--RGLITAFQFLQNYYPERLG 187
             + L     +  +  +   E    I+DL+ IS          +  A    QNYYPER+G
Sbjct: 154 SRYRL----PASSRQADCLVETSCTILDLKGISISAAAQVLSYVREASNIGQNYYPERMG 213

Query: 188 KCFILNMPWFFVSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKA 229
           K +++N P+ F + +R+   F+D  T+  I I+ S  +K  +++++  E LP+++GG++
Sbjct: 214 KFYMINAPFGFSAAFRLFKPFLDPVTVSKIFILGSSYQKE-LLKQIPAENLPVKFGGQS 266

BLAST of CsaV3_5G014390 vs. ExPASy Swiss-Prot
Match: P33324 (CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKL091C PE=1 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 2.7e-12
Identity = 61/245 (24.90%), Postives = 122/245 (49.80%), Query Frame = 0

Query: 8   AVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATVPKGLI-- 67
           A++Q R  + +  +  +   D+T++RFL AR  ++  + +MFV+ ++WR+      +I  
Sbjct: 32  ALLQFRSILLEKNYK-ERLDDSTLLRFLRARKFDINASVEMFVETERWREEYGANTIIED 91

Query: 68  ----AESEVEDELKAKKIF------------------LQGISIKQLPVMIVMANRHFHSK 127
                E+E ++ +K  K++                  L GI++K+   M  +       +
Sbjct: 92  YENNKEAEDKERIKLAKMYPQYYHHVDKDGRPLYFEELGGINLKK---MYKITTEKQMLR 151

Query: 128 DQVQFKKFIVHLLDKVIASGCKGKEIGNEKWIAIVDLQQISYKNVDP--RGLITAFQFLQ 187
           + V  K++ +    +V A   +   +  E    ++DL+ IS  N       +       Q
Sbjct: 152 NLV--KEYELFATYRVPACSRRAGYL-IETSCTVLDLKGISLSNAYHVLSYIKDVADISQ 211

Query: 188 NYYPERLGKCFILNMPWFFVSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLP 227
           NYYPER+GK +I++ P+ F ++++M+  F+D  T+  I I+ S  +K  +++++  E LP
Sbjct: 212 NYYPERMGKFYIIHSPFGFSTMFKMVKPFLDPVTVSKIFILGSSYKKE-LLKQIPIENLP 268

BLAST of CsaV3_5G014390 vs. ExPASy Swiss-Prot
Match: P45816 (SEC14 cytosolic factor OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 6.1e-12
Identity = 56/219 (25.57%), Postives = 109/219 (49.77%), Query Frame = 0

Query: 28  DATMMRFLIARSMEVPKAAKMFVQWKKWRDATVPKGLIAESEVEDELKAKK--------- 87
           DAT++RFL AR  +VP A +M+   +KWR       ++ +   +++ +  K         
Sbjct: 54  DATLLRFLRARKFDVPLAQEMWENCEKWRKEFGTNTILEDFWYKEKKEVAKLYPQYYHKT 113

Query: 88  ------IFLQGISIKQLPVMIVMANRHFHSKDQV-QFKKFIVHLLDKVIASGCKGKEIGN 147
                 ++++ +    +  M  +  +    ++ V +++ F+ H L     S   G  I  
Sbjct: 114 DKDGRPVYVENVGKVNIHEMYKITTQERMLRNLVWEYESFVRHRLP--ACSRVVGHLI-- 173

Query: 148 EKWIAIVDLQQISYKNVDP--RGLITAFQFLQNYYPERLGKCFILNMPWFFVSIWRMISR 207
           E    I+DL+ +S  +       L  A    QNYYPER+GK +++N P+ F +++ +I R
Sbjct: 174 ETSCTILDLKGVSLSSASQVYGFLKDASNIGQNYYPERMGKFYLINAPFGFSTVFSVIKR 233

Query: 208 FVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKA 229
           F+D  T+  I +  S  +++ ++ +V    LPI++GG++
Sbjct: 234 FLDPVTVSKIHVYGSNYKEK-LLAQVPAYNLPIKFGGQS 267

BLAST of CsaV3_5G014390 vs. ExPASy TrEMBL
Match: A0A0A0KL83 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G021320 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.5e-133
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV
Sbjct: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60

Query: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120
           PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI
Sbjct: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180
           ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240
           VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH
Sbjct: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240

Query: 241 LHG 244
           LHG
Sbjct: 241 LHG 243

BLAST of CsaV3_5G014390 vs. ExPASy TrEMBL
Match: A0A1S3BT62 (sec14 cytosolic factor-like OS=Cucumis melo OX=3656 GN=LOC103493455 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.2e-122
Identity = 222/242 (91.74%), Postives = 233/242 (96.28%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           ME S+ANAV  LR+SI KSGFSTQDYSDATMMRFLIARSMEV KAAKMFVQWKKWRDATV
Sbjct: 1   MEDSNANAVTLLRNSIEKSGFSTQDYSDATMMRFLIARSMEVSKAAKMFVQWKKWRDATV 60

Query: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120
           PKG IAESEVEDELKAKKIFLQG+SIKQLPVMIV+ANRHFHSKDQ+QFKKF+VH+LDKVI
Sbjct: 61  PKGYIAESEVEDELKAKKIFLQGMSIKQLPVMIVIANRHFHSKDQLQFKKFVVHILDKVI 120

Query: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180
           ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240
           VSIWRM+SRFVDTATLKNILIVS+EEEKR MIEEVGE+VLPIEYGGKAKF  LQDVV+PH
Sbjct: 181 VSIWRMVSRFVDTATLKNILIVSNEEEKRNMIEEVGEDVLPIEYGGKAKFFALQDVVVPH 240

Query: 241 LH 243
           LH
Sbjct: 241 LH 242

BLAST of CsaV3_5G014390 vs. ExPASy TrEMBL
Match: A0A6J1FSN7 (SEC14 cytosolic factor-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446938 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 4.0e-107
Identity = 198/241 (82.16%), Postives = 215/241 (89.21%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           ME S ANA+IQ+R ++ KSG +TQ+Y DATMMRFLIARSME  K AKMFVQW+KWRDA V
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120
           PKG I+ESEV++EL+AKKIFLQG+S   L VMIVMA RHF SKDQ+QFKKF+VHLLDKVI
Sbjct: 61  PKGFISESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180
           ASG KGKEIGNEKWIAIVDL QISYKNVD RGLIT FQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240
           VSIWRM+SRFVD ATLKNILIVS+EEE+R MIEEVGEEVLP EYGGKAKFI LQD VLPH
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 L 242
           L
Sbjct: 241 L 241

BLAST of CsaV3_5G014390 vs. ExPASy TrEMBL
Match: A0A6J1K6Q5 (SEC14 cytosolic factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491647 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 9.2e-104
Identity = 193/241 (80.08%), Postives = 211/241 (87.55%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           ME S ANA+IQ+R ++ KSG +TQ+Y DATMMRFLIARSME  K AKMFVQW+ WRDA V
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRTWRDAMV 60

Query: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120
           PKG I ESEV++EL+AKKIFLQG+S   L VMIVMA RHF SKDQ+QFKKF+VHLLDKVI
Sbjct: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180
           ASG +GKEIGNEKWIAIVDL QISYKNVD RGLIT FQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSEGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240
           VSIWRM+SRFVD ATLKNILIV ++EE+R MIEEVGEEVLP EYGGKAKFI LQD VLP 
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVRNKEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPR 240

Query: 241 L 242
           L
Sbjct: 241 L 241

BLAST of CsaV3_5G014390 vs. ExPASy TrEMBL
Match: A0A6J1CY34 (sec14 cytosolic factor-like OS=Momordica charantia OX=3673 GN=LOC111015872 PE=4 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.5e-103
Identity = 191/242 (78.93%), Postives = 215/242 (88.84%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           ME S ANA+ Q+R S+ K GFST+ Y DATMMRFLIARSME  K AKMFVQW+KWR+A V
Sbjct: 1   MEESRANALFQMRKSVEKLGFSTEAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMV 60

Query: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120
           P+G + ESEV +EL+A+KIFLQG S K L V+IV A+RHF SKDQ+QFKKF+VHLLDKVI
Sbjct: 61  PEGSVCESEVAEELEARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180
           ASGCKG+EIGNEKWIAIVDL QISYKNVDPRGLIT FQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGREIGNEKWIAIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQD-VVLP 240
           VSIWRM+SRFVD+ATLKNILIVS+EEE+RIMIEEVG EVLP+EYGGKAK I LQD +++P
Sbjct: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVG-EVLPVEYGGKAKLIALQDAIIIP 240

Query: 241 HL 242
           HL
Sbjct: 241 HL 241

BLAST of CsaV3_5G014390 vs. TAIR 10
Match: AT1G14820.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 270.0 bits (689), Expect = 1.8e-72
Identity = 132/240 (55.00%), Postives = 177/240 (73.75%), Query Frame = 0

Query: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60
           ME S   A+ QLR S+ K   ST+ Y   T+MRFL+ARSM+  KAAKMFV W+KWR + V
Sbjct: 1   MEESQELALTQLRKSVEKLSSSTEGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMV 60

Query: 61  -PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKV 120
            P G I ESEV+DEL+ +K+ LQG +    P+++V+ ++HF SKD   FKKF+V+ LDK 
Sbjct: 61  PPTGFIPESEVQDELEFRKVCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKT 120

Query: 121 IASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWF 180
           IASG  GKE+G EK +A++DL  I+YKN+D RGLIT FQFLQ+YYPERL KC+IL+MP F
Sbjct: 121 IASGNNGKEVGGEKLVAVIDLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGF 180

Query: 181 FVSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLP 240
           FV++W+ + RF++ AT + I+IV+  EE+R   EE+G + LP EYGG+AK   +QDV+LP
Sbjct: 181 FVTVWKFVCRFLEKATQEKIVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 240

BLAST of CsaV3_5G014390 vs. TAIR 10
Match: AT1G14820.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 255.0 bits (650), Expect = 6.2e-68
Identity = 122/219 (55.71%), Postives = 165/219 (75.34%), Query Frame = 0

Query: 22  STQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV-PKGLIAESEVEDELKAKKIF 81
           S + Y   T+MRFL+ARSM+  KAAKMFV W+KWR + V P G I ESEV+DEL+ +K+ 
Sbjct: 9   SEKGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVC 68

Query: 82  LQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVIASGCKGKEIGNEKWIAIVDL 141
           LQG +    P+++V+ ++HF SKD   FKKF+V+ LDK IASG  GKE+G EK +A++DL
Sbjct: 69  LQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDL 128

Query: 142 QQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFFVSIWRMISRFVDTATLKNIL 201
             I+YKN+D RGLIT FQFLQ+YYPERL KC+IL+MP FFV++W+ + RF++ AT + I+
Sbjct: 129 ANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIV 188

Query: 202 IVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLP 240
           IV+  EE+R   EE+G + LP EYGG+AK   +QDV+LP
Sbjct: 189 IVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 227

BLAST of CsaV3_5G014390 vs. TAIR 10
Match: AT1G14820.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 255.0 bits (650), Expect = 6.2e-68
Identity = 122/219 (55.71%), Postives = 165/219 (75.34%), Query Frame = 0

Query: 22  STQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV-PKGLIAESEVEDELKAKKIF 81
           S + Y   T+MRFL+ARSM+  KAAKMFV W+KWR + V P G I ESEV+DEL+ +K+ 
Sbjct: 9   SEKGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVC 68

Query: 82  LQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVIASGCKGKEIGNEKWIAIVDL 141
           LQG +    P+++V+ ++HF SKD   FKKF+V+ LDK IASG  GKE+G EK +A++DL
Sbjct: 69  LQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDL 128

Query: 142 QQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFFVSIWRMISRFVDTATLKNIL 201
             I+YKN+D RGLIT FQFLQ+YYPERL KC+IL+MP FFV++W+ + RF++ AT + I+
Sbjct: 129 ANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIV 188

Query: 202 IVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLP 240
           IV+  EE+R   EE+G + LP EYGG+AK   +QDV+LP
Sbjct: 189 IVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 227

BLAST of CsaV3_5G014390 vs. TAIR 10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.5e-37
Identity = 78/214 (36.45%), Postives = 130/214 (60.75%), Query Frame = 0

Query: 23  TQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATVPKGLIAESEVEDELKAKKIFLQ 82
           T++  D  + RFL AR +++ KA+ MF+ +  W+ + +PKG I E+E+ ++L   K+ +Q
Sbjct: 45  TKEVDDLMIRRFLRARDLDIEKASTMFLNYLTWKRSMLPKGHIPEAEIANDLSHNKMCMQ 104

Query: 83  GISIKQLPVMIVMANRHFHSK-DQVQFKKFIVHLLDKVIASGCKGKEIGNEKWIAIVDLQ 142
           G      P+ + + NRH  SK +  +FK+F+V+ L+K+    C     G EK++AI DLQ
Sbjct: 105 GHDKMGRPIAVAIGNRHNPSKGNPDEFKRFVVYTLEKI----CARMPRGQEKFVAIGDLQ 164

Query: 143 QISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFFVSIWRMISRFVDTATLKNILI 202
              Y N D RG + A   LQ+ YPERLGK +I++ P+ F++ W++I  F+D  T K I+ 
Sbjct: 165 GWGYSNCDIRGYLAALSTLQDCYPERLGKLYIVHAPYIFMTAWKVIYPFIDANTKKKIVF 224

Query: 203 VSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQD 236
           V +++    ++E++ E  LP  YGGK   + +Q+
Sbjct: 225 VENKKLTPTLLEDIDESQLPDIYGGKLPLVPIQE 254

BLAST of CsaV3_5G014390 vs. TAIR 10
Match: AT4G36640.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 83.2 bits (204), Expect = 3.2e-16
Identity = 70/226 (30.97%), Postives = 110/226 (48.67%), Query Frame = 0

Query: 7   NAVIQLRDSIHK-SGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATVPKGLI 66
           N V +L+ +I   SG S    SDA++ RFL AR+ +V KA KM  +  KWR    P+  I
Sbjct: 19  NKVRELKSAIGPLSGHSLVFCSDASLRRFLDARNWDVEKAKKMIQETLKWRSTYKPQE-I 78

Query: 67  AESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVIASGCK 126
             ++V  E +  K        +Q  V+++M     +S  Q    + +V+LL+  I +  K
Sbjct: 79  RWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQEGNIRHLVYLLENAIINLPK 138

Query: 127 GKEIGNEKWIAIVDLQQISYK-NVDPRGLITAFQFLQNYYPERLGKCFILNMPWFFVSIW 186
           G++     W  ++D    S   N   +        LQNYYPERLG  F+ N P  F +++
Sbjct: 139 GQK--QMSW--LIDFTGWSMAVNPPMKTTREIIHILQNYYPERLGIAFLYNPPRLFQAVY 198

Query: 187 RMISRFVDTATLKNILIVSSEEE--KRIMIEEVGEEVLPIEYGGKA 229
           R    F+D  T + +  V  +++    +M      E LP E+GG+A
Sbjct: 199 RAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGEA 239