Homology
BLAST of CsaV3_5G003440 vs. NCBI nr
Match:
KGN50165.1 (hypothetical protein Csa_000664 [Cucumis sativus])
HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 745/745 (100.00%), Postives = 745/745 (100.00%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN
Sbjct: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
Query: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR
Sbjct: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
Query: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
Query: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG
Sbjct: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
Query: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST
Sbjct: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
Query: 721 EFAEGYLKWVSAKHLVRSPISVKFV 746
EFAEGYLKWVSAKHLVRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHLVRSPISVKFV 745
BLAST of CsaV3_5G003440 vs. NCBI nr
Match:
KAA0042818.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])
HSP 1 Score: 1421.4 bits (3678), Expect = 0.0e+00
Identity = 713/745 (95.70%), Postives = 730/745 (97.99%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
MVLLPSLFLLLLLNFH YEAQVTELP SNLHTYIVHVKKPEVVDDLE WHRSFLPTSL+N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
EEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTH+P+FLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITP+HPSF DAGMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RS+NLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
HLAIYKVCFGE CS+VDILAGLDAAVEDGVDVLSISLGGP VPFFADITAIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALCAEGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
LLNAESDGFTTE DAHVLPASHVSHTAALKIKAYINSTTYPTATI+FKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNI+SGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPI+D+TLQPADLFAIGAGHVNPSKA+D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTT+SIPEGELNYPSFMVKLG VQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
TFSRTVT VGSGR VYNVVIEAPEGVSVTVRPRK+ FSALNQKATYSVTFKRIGSISPS
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
Query: 721 EFAEGYLKWVSAKHLVRSPISVKFV 746
EFAEGYLKWVSAKH+VRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of CsaV3_5G003440 vs. NCBI nr
Match:
XP_008437181.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])
HSP 1 Score: 1416.0 bits (3664), Expect = 0.0e+00
Identity = 711/745 (95.44%), Postives = 728/745 (97.72%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
MVLLPSLFLLLLLNFH YEAQVTELP SNLHTYIVHVKKPEVVDDLE WHRSFLPTSL+N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
EEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTH+P+FLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITP+HPSF DAGM QPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RS+NLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
HLAIYKVCFGE CS+VDILAGLDAAVEDGVDVLSISLGGP VPFFADITAIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALCAEGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
LLNAESDGFTTE DAHVLPASHVSHTAALKIKAYINSTTYPTATI+FKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNI+SGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPI+D+TLQPADLFAIGAGHVNPSKA+D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTT+SIPEGELNYPSFMVKLG VQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
TFSRTVT VGSGR VYNVVIEAPEGVSVTVRPRK+ FSALNQKATYSVTFKR GSISPS
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPSI 720
Query: 721 EFAEGYLKWVSAKHLVRSPISVKFV 746
EFAEGYLKWVSAKH+VRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of CsaV3_5G003440 vs. NCBI nr
Match:
XP_038876087.1 (LOW QUALITY PROTEIN: subtilisin-like protease 4 [Benincasa hispida])
HSP 1 Score: 1293.5 bits (3346), Expect = 0.0e+00
Identity = 643/727 (88.45%), Postives = 672/727 (92.43%), Query Frame = 0
Query: 25 LPFSN------LHTYIVHVKKPEVVDDLESWHRSFLPTSLENSEEQPTLLYSYRNVMSGF 84
LPFS+ LHTYIVHVKKPE DDLESWHRSFLPTSLENSEEQP+LLYSYRNVMSGF
Sbjct: 17 LPFSHIKLPLCLHTYIVHVKKPETADDLESWHRSFLPTSLENSEEQPSLLYSYRNVMSGF 76
Query: 85 SARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNRQFGFWKDSNFGKGVIIGV 144
+ARLTEE VKAMEEKDGFVSARRE I+HLHTTH+P+FLGLNRQFGFWKDSNFGKGVIIGV
Sbjct: 77 AARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGV 136
Query: 145 LDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGARSLNLASQALKGKITTLD 204
LDGGITP+HPSF D GMPQPP KWKGRCEFNFSACNNKLIGARS NLAS+ALKG TT+D
Sbjct: 137 LDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGARSFNLASKALKGHATTMD 196
Query: 205 DSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGESCSNVDI 264
DSPIDEDGHGTHTASTAAGTF+DGAEALGNA GTA GMAPLAHLAIYKVCFGE C + DI
Sbjct: 197 DSPIDEDGHGTHTASTAAGTFIDGAEALGNAIGTAXGMAPLAHLAIYKVCFGEDCPDTDI 256
Query: 265 LAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKGIFVSCSAANSGPFNATLS 324
LA LDAAVEDGVDVLS+SLG P PFF D AIGAF AIQKGIFV CSAANSGPFNATLS
Sbjct: 257 LAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRCSAANSGPFNATLS 316
Query: 325 NEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKNETVAL 384
NEAPWILTVAASTIDR+I A AKLGNGEE DGESLFQPNDFPQTFLPLVFPGEKNETVAL
Sbjct: 317 NEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFPQTFLPLVFPGEKNETVAL 376
Query: 385 CAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILLNAESDGFTTEADAHVL 444
C EGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKNAGGAAMILLN ESDGFTTEADAHVL
Sbjct: 377 CGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNTESDGFTTEADAHVL 436
Query: 445 PASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAIAAFSSRGPSLASPGILKP 504
PASHVSHTAALKIKAYINSTTYP ATIVFKGT IGDDFSPAIA+FSSRGPSLASPGILKP
Sbjct: 437 PASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKP 496
Query: 505 DITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSGIAALIKSAHPDWSPAAIK 564
DITGPGVSILAAWPFPLDNNTN K TFNIVSGTSMSCPHLSGIAALIKS+HPDWSPA IK
Sbjct: 497 DITGPGVSILAAWPFPLDNNTNPKXTFNIVSGTSMSCPHLSGIAALIKSSHPDWSPAVIK 556
Query: 565 SSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVDPGLVYDIQPDDYIPYLCG 624
SSIMTTANITNLEGNPIVDQTLQPADLFA GAGHVNPSKA DPGLVYDIQPDDYIPYLCG
Sbjct: 557 SSIMTTANITNLEGNPIVDQTLQPADLFATGAGHVNPSKANDPGLVYDIQPDDYIPYLCG 616
Query: 625 LGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQTFSRTVTYVGSGREVYNV 684
LGY NNQVSL+AHKP++CLTT SIPEGELNYPSF V+LG QTF+RTVT VG GREVY V
Sbjct: 617 LGYKNNQVSLVAHKPVECLTTPSIPEGELNYPSFTVELGPPQTFTRTVTNVGGGREVYTV 676
Query: 685 VIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPSTEFAEGYLKWVSAKHLVRS 744
V+EAP VSVTV+PR +IFSALNQKATY+VTFKRIGSISPS EFA+GY+KWVS KH+VRS
Sbjct: 677 VVEAPAAVSVTVKPRTLIFSALNQKATYAVTFKRIGSISPSIEFAKGYIKWVSTKHVVRS 736
Query: 745 PISVKFV 746
PIS+KFV
Sbjct: 737 PISIKFV 743
BLAST of CsaV3_5G003440 vs. NCBI nr
Match:
XP_023532974.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 614/745 (82.42%), Postives = 662/745 (88.86%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
MV PSLFLLLLLNFH A VTELP NL TYIVHV+KPE DDLESWHRSFLP+S
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVEKPETTDDLESWHRSFLPSS--- 60
Query: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
+LLYSYRNVMSGF+ARL+EE VKAMEEKDGFVSARRE I+ LHTTH+P+FLGLNR
Sbjct: 61 ----SSLLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILQLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI PSHPSF D GMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RS NLA++ LKG+ T +DDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSFNLATKVLKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
Query: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
HLAIYKVCFGE C + DILA LDAA+EDGVDVLS+SLG P VPFF D+ AIGAFAAIQKG
Sbjct: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPF ATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
Query: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
TFLPLV+ GEKN+T ALC EGSLK+IDVKGK+VVC+RGGGIARIAKG EVKNAGGAAMI
Sbjct: 361 PTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKIVVCERGGGIARIAKGTEVKNAGGAAMI 420
Query: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDD-FSPA 480
LLN + DGF+TEADAHVLPASHVSH AALKIKAYINSTTYPTATI+FKGT IGDD FSPA
Sbjct: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
Query: 481 IAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLS 540
IA+FSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N+NTKSTFNI+SGTSMSCPHLS
Sbjct: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNSNTKSTFNIISGTSMSCPHLS 540
Query: 541 GIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAV 600
GIAALIKS+HPDWSPAAIKS+IMTTA+ITNLEG PIVD+ LQPADLFA GAGHVNPSKA
Sbjct: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
Query: 601 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQV 660
DPGLVYDIQPDDYIPYLCGLGY +N+V+ IA KPI+CL SIPEG+LNYPSF V LG
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
Query: 661 QTFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPS 720
QTF+RTVT VG GREVY V+EAP +SVT+RP K+ FS +N+K TYSVTFKRIGSISPS
Sbjct: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINEKVTYSVTFKRIGSISPS 720
Query: 721 TEFAEGYLKWVSAKHLVRSPISVKF 745
TEF +GYLKWVS KH+VRSPIS KF
Sbjct: 721 TEFGKGYLKWVSDKHVVRSPISFKF 737
BLAST of CsaV3_5G003440 vs. ExPASy Swiss-Prot
Match:
A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)
HSP 1 Score: 977.2 bits (2525), Expect = 1.0e-283
Identity = 493/752 (65.56%), Postives = 592/752 (78.72%), Query Frame = 0
Query: 9 LLLLLNFHVYEAQVTELP--------FSNLHTYIVHVKKPE-----VVDDLESWHRSFLP 68
L LL FHV+ AQ +ELP S+ YI+HV PE +DLESW+ SFLP
Sbjct: 9 LTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLESWYHSFLP 68
Query: 69 TSLENSEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNF 128
+L +SEEQP ++YSY+NV+ GF+A LT+E + A+E+K+GF+SA + ++H TTH+P F
Sbjct: 69 PTLMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTTHTPKF 128
Query: 129 LGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNN 188
LGL + G WK+SNFGKGVIIGVLD GITP HPSF D G+P PP KWKGRC+ N +ACNN
Sbjct: 129 LGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNVTACNN 188
Query: 189 KLIGARSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVG 248
KLIGAR+ NLA++A+ GK ++PIDEDGHGTHTASTAAG FV+ AE LGNA GTA G
Sbjct: 189 KLIGARAFNLAAEAMNGKKA---EAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKGTAAG 248
Query: 249 MAPLAHLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLG-GPPVPFFADITAIGAF 308
MAP AHLAIYKVCFGE C DILA LDAAVEDGVDV+SISLG P PFF D TAIGAF
Sbjct: 249 MAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTAIGAF 308
Query: 309 AAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLF 368
AA+QKGIFVSC+A NSGPFN+++ N APWILTV ASTIDR+I ATAKLGNG+EFDGES+F
Sbjct: 309 AAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDGESVF 368
Query: 369 QPNDFPQTFLPLVFPGEK-NETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKN 428
QP+ F T LPL + G+ E A CA GSL + +GKVV+C+RGGGIARIAKG EVK
Sbjct: 369 QPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGEEVKR 428
Query: 429 AGGAAMILLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIG 488
AGGAAMIL+N E++ F+ AD H LPA+HVS+ A ++IKAYINST PTATI+FKGT IG
Sbjct: 429 AGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKGTVIG 488
Query: 489 DDFSPAIAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSM 548
+ +PA+A+FSSRGP+L SPGILKPDI GPGV+ILAAWPFPL N+T++K TFNI SGTSM
Sbjct: 489 NSLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSKLTFNIESGTSM 548
Query: 549 SCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHV 608
SCPHLSGIAAL+KS+HP WSPAAIKS+IMT+A+ NL IVD+TLQP DLFA G+GHV
Sbjct: 549 SCPHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTDLFATGSGHV 608
Query: 609 NPSKAVDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFM 668
NPS+A DPGLVYDIQPDDYIPYLCGLGY+ +V +IAH+ I C + SIPEGELNYPSF
Sbjct: 609 NPSRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKC--SASIPEGELNYPSFS 668
Query: 669 VKLGQVQTFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRI 728
V+LG +TF+RTVT VG Y++++ AP+GV V V+P K+ FS +NQK TYSVTF R
Sbjct: 669 VELGSSKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVNQKETYSVTFSRT 728
Query: 729 GSISPSTEFAEGYLKWVSAKHLVRSPISVKFV 746
G + + E+A+G+LKWVS KH VRSPISVKF+
Sbjct: 729 GLGNKTQEYAQGFLKWVSTKHTVRSPISVKFI 755
BLAST of CsaV3_5G003440 vs. ExPASy Swiss-Prot
Match:
A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)
HSP 1 Score: 860.9 bits (2223), Expect = 1.1e-248
Identity = 445/742 (59.97%), Postives = 548/742 (73.85%), Query Frame = 0
Query: 9 LLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEV-----VDDLESWHRSFLPTSLENSEE 68
+ LL+FH+ AQ + + L TYIVHV KP+ DLES++ SFLP ++ SE
Sbjct: 9 IAFLLSFHLTTAQRS----TGLQTYIVHVDKPDAQVLANSADLESYYNSFLPATVSGSEV 68
Query: 69 QPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNRQFG 128
+++SY +V +GF+A+L+ E VK ME+K GFVSA+ E ++ LHTTH+PNFLGL + G
Sbjct: 69 PSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMG 128
Query: 129 FWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSA-CNNKLIGARS 188
FW++SN+GKGVIIG+LD GITP HPSF D MP PPAKWKG+CEF +A CN K+IGAR+
Sbjct: 129 FWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARN 188
Query: 189 LNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHL 248
I+ P DE+GHGTHTASTAAG FV+ A GNA GTAVGMAPLAH+
Sbjct: 189 F----------ISGSGVPPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAHI 248
Query: 249 AIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKGIF 308
A+YKVC + CS+ DILA LDAA++DGVDVLS+SLGG PF+ D AIGAFAAI+KGIF
Sbjct: 249 AMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIF 308
Query: 309 VSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQT 368
VS SA N GP N+TLSNEAPWILTV AST DRKI ATA LGNG+++DGES FQP DFP T
Sbjct: 309 VSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPHT 368
Query: 369 FLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILL 428
LPLV+PG +E A C+ GSL DVKGKVVVCDRGG +AR+ K VK+AGGAAMIL
Sbjct: 369 LLPLVYPGTSDEEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMILA 428
Query: 429 NAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAIAA 488
N E DG T ADAHVLPA+HV + A IK+YINST+ PTA I+FKGT IG SP++++
Sbjct: 429 NLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKSSPSVSS 488
Query: 489 FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSGIA 548
FSSRGP+LASPGI+KPDI GPGV+ILAAWP ++N T T TFNI+SGTSMSCPHLSGI
Sbjct: 489 FSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIISGTSMSCPHLSGIV 548
Query: 549 ALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVDPG 608
AL+KSAHPDWSPAAIKS+IMT+A+ +NLEG PI+D+ PAD+FA GAGHVNPSKA DPG
Sbjct: 549 ALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKASDPG 608
Query: 609 LVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLG-QVQT 668
L+YDIQ +DYI YLCGLGY + LI + + C +SI E ELNYPSF + LG + Q
Sbjct: 609 LIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKC--ESSISEAELNYPSFSIILGPKTQN 668
Query: 669 FSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPSTE 728
++RTVT VG Y V I +GV + V P ++F+ + Q+ATY+V+F + G +
Sbjct: 669 YTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTVSFTQSGDF--TDR 728
Query: 729 FAEGYLKWVSAKHLVRSPISVK 744
F +G + W S +++VRSPISVK
Sbjct: 729 FVQGAISWSSNEYVVRSPISVK 732
BLAST of CsaV3_5G003440 vs. ExPASy Swiss-Prot
Match:
A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)
HSP 1 Score: 832.0 bits (2148), Expect = 5.2e-240
Identity = 441/729 (60.49%), Postives = 532/729 (72.98%), Query Frame = 0
Query: 29 NLHTYIVHVKKPEV------VDDLESWHRSFLPTSLENSEEQPTLLYSYRNVMSGFSARL 88
NL TYIVHVKK E+ ++L +WH SFLP + + +++SYRNV SGF+ RL
Sbjct: 37 NLTTYIVHVKKLEIEGPLQSTEELHTWHHSFLP----ETSNKDRMVFSYRNVASGFAVRL 96
Query: 89 TEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNRQFGFWKDSNFGKGVIIGVLDGG 148
T E A++EK+ +S R E + LHTTH+P+FLGL + G W DSN GKGVIIGV+D G
Sbjct: 97 TPEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTG 156
Query: 149 ITPSHPSFVDAGMPQPPAKWKGRCEF-NFSACNNKLIGARSLNLASQALKGKITTLDDSP 208
I P H SF D GMP PPAKWKG CEF S CNNKLIGAR NL A++ + P
Sbjct: 157 IYPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ-------EPP 216
Query: 209 IDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFG---ESCSNVDI 268
++ HGTHTA+ AAG FV+GA GNA GTA GMAP AHLAIYKVC + C I
Sbjct: 217 YEDFFHGTHTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAI 276
Query: 269 LAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKGIFVSCSAANSGPFNATLS 328
LA +D A+EDGVDVLS+SLG +PFF D AIGAFAA QKGIFVSCSAANSGP ++LS
Sbjct: 277 LAAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLS 336
Query: 329 NEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTFLPLVF-PGEKNETVA 388
NEAPWILTV ASTIDRKI+A+AKLGNG E++GE+LFQP DF LPLV+ EKN + A
Sbjct: 337 NEAPWILTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSA 396
Query: 389 LCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILLNAESDGFTTEADAHV 448
LCA GSL+NI+VKGKVVVCD GGGI IAKG EV +AGG+AMIL N E+ GFTT A+AHV
Sbjct: 397 LCAPGSLRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHV 456
Query: 449 LPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAIAAFSSRGPSLASPGILK 508
LPA HVS+ A+L IKAYINST PTAT++F+GT IGD +P++AAFSSRGPS SPGILK
Sbjct: 457 LPAVHVSYAASLAIKAYINSTYTPTATVLFQGTIIGDSLAPSVAAFSSRGPSQQSPGILK 516
Query: 509 PDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSGIAALIKSAHPDWSPAAI 568
PDI GPGV+ILAAW +DN F+I+SGTSMSCPHLSGIAAL+KSAHPDWSPAAI
Sbjct: 517 PDIIGPGVNILAAWAVSVDNKI---PAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAI 576
Query: 569 KSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVDPGLVYDIQPDDYIPYLC 628
KS+IMTTAN NL G PI+DQ LQPAD+FA GAGHVNP +A DPGLVYDIQP+DY+PYLC
Sbjct: 577 KSAIMTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLC 636
Query: 629 GLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLG-QVQTFSRTVTYVGSGREVY 688
GLGY++ +V++I + + C SI + ELNYPSF + LG Q ++RT+T VG Y
Sbjct: 637 GLGYSDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTY 696
Query: 689 NVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTF-KRIGSISPSTEFAEGYLKWVSAKHL 745
V I+ P + ++V P ++ F+ +NQK Y V F +I + FA+G + WVS KH+
Sbjct: 697 TVKIDVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHV 749
BLAST of CsaV3_5G003440 vs. ExPASy Swiss-Prot
Match:
A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)
HSP 1 Score: 810.4 bits (2092), Expect = 1.6e-233
Identity = 433/753 (57.50%), Postives = 536/753 (71.18%), Query Frame = 0
Query: 7 LFLLLLLNFHVYEAQVTELPF--------SNLHTYIVHVKKPEVV--DDLESWHRSFLPT 66
L L+L+L+ + A L F SNL TYIVHV+KP+V+ DDL +++ S LP
Sbjct: 11 LALILVLSVSLASAASQNLEFTELEDEDQSNLSTYIVHVRKPQVIQSDDLHTFYYSLLPE 70
Query: 67 SLENSEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFL 126
S + + ++ ++++YRNV++GF+ +LT E KA+++ + VSAR E I+ LHTTH+P+FL
Sbjct: 71 STKTTNQR--IVFTYRNVVNGFAVKLTPEEAKALQQNEEVVSARPEKILSLHTTHTPSFL 130
Query: 127 GLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFS-ACNN 186
GL + G WK SN GKGVIIG+LD GI+P HPSF D GMP PPAKW G CEF CNN
Sbjct: 131 GLQQGLGLWKGSNSGKGVIIGILDTGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNN 190
Query: 187 KLIGARSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVG 246
K+IGAR+ +K K TL P D+ GHGTHTASTAAG V GA GNA GTAVG
Sbjct: 191 KIIGARNF------VKTKNLTL---PFDDVGHGTHTASTAAGRPVQGANVYGNANGTAVG 250
Query: 247 MAPLAHLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFA 306
MAP AH+A+YKVC CS ILAG+D AV+DGVDVLS+SLGGP PFF D A+GAF
Sbjct: 251 MAPDAHIAMYKVCGLVGCSESAILAGMDTAVDDGVDVLSLSLGGPSGPFFEDPIALGAFG 310
Query: 307 AIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQ 366
AIQKGIFVSCSAANSGP ++LSNEAPWILTV AS+IDR I ATAKLGNG+E+ G+S+FQ
Sbjct: 311 AIQKGIFVSCSAANSGPAYSSLSNEAPWILTVGASSIDRTIMATAKLGNGKEYVGQSVFQ 370
Query: 367 PNDFPQTFLPLVFPGE--KNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKN 426
P DF + LPLV+ G N CA SL DV+GKVV+C+ GG + R+ KG VK+
Sbjct: 371 PKDFAPSLLPLVYAGANGNNNFSVFCAPESLNRSDVEGKVVLCEDGGFVPRVFKGKAVKD 430
Query: 427 AGGAAMILLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIG 486
AGGAAMIL+N+ + F AD HVLPA H+S+ A L +K YINST+ PTATI+F+GT IG
Sbjct: 431 AGGAAMILMNSVLEDFNPIADVHVLPAVHISYEAGLALKEYINSTSTPTATILFEGTVIG 490
Query: 487 DDFSPAIAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSM 546
+ +P + +FSSRGPS ASPGILKPDI GPG++ILAAWP LDN+T FNI+SGTSM
Sbjct: 491 NLLAPQVTSFSSRGPSKASPGILKPDIIGPGLNILAAWPVSLDNSTT--PPFNIISGTSM 550
Query: 547 SCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHV 606
SCPHLSGIAAL+K++HPDWSPAAIKS+IMTTA+ NL G PI+DQ L PAD+FA GAGHV
Sbjct: 551 SCPHLSGIAALLKNSHPDWSPAAIKSAIMTTASQVNLGGTPILDQRLVPADVFATGAGHV 610
Query: 607 NPSKAVDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFM 666
NP KA DPGLVYDI+P+DYIPYLCGL YT+ +V +I + + C I E ELNYPSF
Sbjct: 611 NPVKANDPGLVYDIEPNDYIPYLCGLNYTDREVGVILQQRVRCSEVNHIAEAELNYPSFS 670
Query: 667 VKLGQ-VQTFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKR 726
+ LG Q ++RTV VG Y I P GV +++ P ++ F+ + QK TYSV+F
Sbjct: 671 ILLGNTTQLYTRTVANVGPANSTYTAEIGVPVGVGMSLSPAQLTFTEVGQKLTYSVSFIP 730
Query: 727 IGSISPSTEFAEGYLKWVSAKHLVRSPISVKFV 746
+ FA+G LKWVS K+ VRSPIS F+
Sbjct: 731 FSEDRDNHTFAQGSLKWVSGKYSVRSPISFIFL 750
BLAST of CsaV3_5G003440 vs. ExPASy Swiss-Prot
Match:
G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)
HSP 1 Score: 793.1 bits (2047), Expect = 2.7e-228
Identity = 431/756 (57.01%), Postives = 525/756 (69.44%), Query Frame = 0
Query: 3 LLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVV-----DDLESWHRSFLPTS 62
LL SL +L + + E NL TYIVHVKK E V +DL SW+ SFLP +
Sbjct: 8 LLVSLIFILCSISMLAAEENLEHDQINLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQT 67
Query: 63 LENSEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLG 122
+ E +++SYR V SGF+ +LT E K+++EK VSAR E + LHTTH+P FLG
Sbjct: 68 FPHKER---MVFSYRKVASGFAVKLTPEEAKSLQEKGEIVSARPERTLELHTTHTPTFLG 127
Query: 123 LNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEF-NFSACNNK 182
L + G W D N GKGVIIG++D GI P HPSF D GMP PPAKWKG CEF CNNK
Sbjct: 128 LKQGQGLWSDDNLGKGVIIGIIDTGIFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNK 187
Query: 183 LIGARSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGM 242
LIGAR NL A++ + P + HGTHTA+ AAG F++ A GNA G A GM
Sbjct: 188 LIGAR--NLVKSAIQ-------EPPFENFFHGTHTAAEAAGRFIEDASVFGNAKGVAAGM 247
Query: 243 APLAHLAIYKVCFGE-SCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFA 302
AP AHLAIYKVC + C+ ILA +D A+EDGVDVLS+SLG +PFF D AIGAFA
Sbjct: 248 APNAHLAIYKVCNDKIGCTESAILAAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFA 307
Query: 303 AIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQ 362
A Q G+FVSCSAANSGP +TLSNEAPWILTV ASTIDRKI A+AKLGNGEE++GE+LFQ
Sbjct: 308 ATQNGVFVSCSAANSGPGYSTLSNEAPWILTVGASTIDRKIVASAKLGNGEEYEGETLFQ 367
Query: 363 PNDFPQTFLPLVFP-----GEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVE 422
P DF Q LPLV+P G + + +LC GSLKNID+ GKVV+CD G ++ I KG E
Sbjct: 368 PKDFSQQLLPLVYPGSFGYGNQTQNQSLCLPGSLKNIDLSGKVVLCD-VGNVSSIVKGQE 427
Query: 423 VKNAGGAAMILLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGT 482
V N+GG AMIL N+E+ GF+T A AHVLPA VS+ A L IK+YI ST PTAT++FKGT
Sbjct: 428 VLNSGGIAMILANSEALGFSTFAIAHVLPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGT 487
Query: 483 TIGDDFSPAIAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSG 542
IGD +P++ FSSRGPS SPGILKPDI GPGV+ILAAW +DN F+IVSG
Sbjct: 488 IIGDSLAPSVVYFSSRGPSQESPGILKPDIIGPGVNILAAWAVSVDNKI---PAFDIVSG 547
Query: 543 TSMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGA 602
TSMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTAN NL G PI+DQ L PAD+FA GA
Sbjct: 548 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANTLNLGGIPILDQRLFPADIFATGA 607
Query: 603 GHVNPSKAVDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYP 662
GHVNP KA DPGLVYDI+P+DY+PYLCGLGY++ ++ +I + C SIPE +LNYP
Sbjct: 608 GHVNPVKANDPGLVYDIEPEDYVPYLCGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYP 667
Query: 663 SFMVKLG-QVQTFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVT 722
SF + LG Q ++RT+T VG Y V +E P + ++V P ++ F+ +N+K ++SV
Sbjct: 668 SFSILLGSDSQYYTRTLTNVGFANSTYKVELEVPLALGMSVNPSEITFTEVNEKVSFSVE 727
Query: 723 F-KRIGSISPSTEFAEGYLKWVSAKHLVRSPISVKF 745
F +I + F +G L WVS +H VR PISV F
Sbjct: 728 FIPQIKENRRNHTFGQGSLTWVSDRHAVRIPISVIF 747
BLAST of CsaV3_5G003440 vs. ExPASy TrEMBL
Match:
A0A0A0KKE3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1)
HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 745/745 (100.00%), Postives = 745/745 (100.00%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN
Sbjct: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
Query: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR
Sbjct: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
Query: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
Query: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG
Sbjct: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
Query: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST
Sbjct: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
Query: 721 EFAEGYLKWVSAKHLVRSPISVKFV 746
EFAEGYLKWVSAKHLVRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHLVRSPISVKFV 745
BLAST of CsaV3_5G003440 vs. ExPASy TrEMBL
Match:
A0A5A7TJ55 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003250 PE=3 SV=1)
HSP 1 Score: 1421.4 bits (3678), Expect = 0.0e+00
Identity = 713/745 (95.70%), Postives = 730/745 (97.99%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
MVLLPSLFLLLLLNFH YEAQVTELP SNLHTYIVHVKKPEVVDDLE WHRSFLPTSL+N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
EEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTH+P+FLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITP+HPSF DAGMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RS+NLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
HLAIYKVCFGE CS+VDILAGLDAAVEDGVDVLSISLGGP VPFFADITAIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALCAEGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
LLNAESDGFTTE DAHVLPASHVSHTAALKIKAYINSTTYPTATI+FKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNI+SGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPI+D+TLQPADLFAIGAGHVNPSKA+D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTT+SIPEGELNYPSFMVKLG VQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
TFSRTVT VGSGR VYNVVIEAPEGVSVTVRPRK+ FSALNQKATYSVTFKRIGSISPS
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
Query: 721 EFAEGYLKWVSAKHLVRSPISVKFV 746
EFAEGYLKWVSAKH+VRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of CsaV3_5G003440 vs. ExPASy TrEMBL
Match:
A0A1S3ATZ7 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=1)
HSP 1 Score: 1416.0 bits (3664), Expect = 0.0e+00
Identity = 711/745 (95.44%), Postives = 728/745 (97.72%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
MVLLPSLFLLLLLNFH YEAQVTELP SNLHTYIVHVKKPEVVDDLE WHRSFLPTSL+N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
EEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTH+P+FLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITP+HPSF DAGM QPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RS+NLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
HLAIYKVCFGE CS+VDILAGLDAAVEDGVDVLSISLGGP VPFFADITAIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALCAEGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
LLNAESDGFTTE DAHVLPASHVSHTAALKIKAYINSTTYPTATI+FKGTTIGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNI+SGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPI+D+TLQPADLFAIGAGHVNPSKA+D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTT+SIPEGELNYPSFMVKLG VQ
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
TFSRTVT VGSGR VYNVVIEAPEGVSVTVRPRK+ FSALNQKATYSVTFKR GSISPS
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPSI 720
Query: 721 EFAEGYLKWVSAKHLVRSPISVKFV 746
EFAEGYLKWVSAKH+VRSPISVKFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of CsaV3_5G003440 vs. ExPASy TrEMBL
Match:
A0A6J1H242 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE=3 SV=1)
HSP 1 Score: 1219.9 bits (3155), Expect = 0.0e+00
Identity = 614/745 (82.42%), Postives = 659/745 (88.46%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
MV PSLFLLLLLNFH A VTELP NL TYIVHVKKPE DDLE WHRSFLP+S
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSS--- 60
Query: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
+LLYSYRNVMSGF+ARL+EE VKAMEE DGFVSARRE I+ LHTTH+P+FLGLNR
Sbjct: 61 ----SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI PSHPSF D GMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
RS NLA++ALKG+ T +DDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
Query: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
HLAIYKVCFGE C + DILA LDAA+EDGVDVLS+SLG P VPFF D+ AIGAFAAIQKG
Sbjct: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPF ATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
Query: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
T LPLV+ GEKN+T ALC EGSLK+IDVKGKVVVC+RGGGIARIAKG EVKNAGGAAMI
Sbjct: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
Query: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDD-FSPA 480
LLN + DGF+TEADAHVLPASHVSH AALKIKAYINSTTYPTATI+FKGT IGDD FSPA
Sbjct: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
Query: 481 IAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLS 540
IA+FSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNI+SGTSMSCPHLS
Sbjct: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLS 540
Query: 541 GIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAV 600
GIAALIKS+HPDWSPAAIKS+IMTTA+ITNLEG PIVD+ LQPADLFA GAGHVNPSKA
Sbjct: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
Query: 601 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQV 660
DPGLVYDIQPDDYIPYLCGLGY +N+V+ IA KPI+CL SIPEG+LNYPSF V LG
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
Query: 661 QTFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPS 720
QTF+RTVT VG GREVY V+EAP +SVT+RP K+ FS +NQK T+SVTFKRIGSISPS
Sbjct: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPS 720
Query: 721 TEFAEGYLKWVSAKHLVRSPISVKF 745
TEF +GYLKWVS KH+VRSPIS KF
Sbjct: 721 TEFGKGYLKWVSDKHVVRSPISFKF 737
BLAST of CsaV3_5G003440 vs. ExPASy TrEMBL
Match:
A0A6J1E0E3 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 PE=3 SV=1)
HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 602/756 (79.63%), Postives = 658/756 (87.04%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPE------VVDDLESWHRSFL 60
MV+LPSLFLL+L +FH A+ T SNL TYIVHVKKPE V+DLE+WHRSFL
Sbjct: 1 MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
Query: 61 PTSLENSEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPN 120
P+S SE+ P LLYS+ NVMSGF+ARLTEEHVKAMEEKDGF+SARRE I+ LHTTH+PN
Sbjct: 61 PSS---SEDHPKLLYSFXNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPN 120
Query: 121 FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACN 180
FLGLNRQFGFWKDSNFGKG IIGVLDGGITP+HPSF DAGMP PP KWKGRCEFNFSACN
Sbjct: 121 FLGLNRQFGFWKDSNFGKGXIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACN 180
Query: 181 NKLIGARSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAV 240
NKLIGARS NLA++ALKG+ T DSPIDEDGHGTHTASTAAG FVD AEALGNA GTAV
Sbjct: 181 NKLIGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAV 240
Query: 241 GMAPLAHLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAF 300
GMAPLAHLAIYKVCFGE C + DILA LDAAVEDGVDVLS+SLG PFF D AIGAF
Sbjct: 241 GMAPLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAF 300
Query: 301 AAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLF 360
AA QKGIFVSCSA+NSGP NATLSNEAPWILTVAASTIDR+I ATAKLGNGEEFDGESLF
Sbjct: 301 AATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLF 360
Query: 361 QPNDFPQT-FLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKN 420
QP+DFP T FLPLV+ GEKNET ALC EGSLK+IDVKGK VVC+RGGGIARIAKG+EVKN
Sbjct: 361 QPSDFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKN 420
Query: 421 AGGAAMILLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIG 480
AGGAAMILLN ++DGFTTEADAHVLPA+HV+H AALKIK YINST P A I F+GT IG
Sbjct: 421 AGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIG 480
Query: 481 -DDFSPAIAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNT-NTKSTFNIVSGT 540
DDFSPAIA+FSSRGPSLASPGILKPDITGPG+SILAAWPFPLDNNT NTK TFN++SGT
Sbjct: 481 DDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGT 540
Query: 541 SMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAG 600
SMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTA+ITN +G PI+DQ QPADLFA GAG
Sbjct: 541 SMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAG 600
Query: 601 HVNPSKAVDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPS 660
HVNPSKA DPGLVYDIQPDDYIPYLCGLGY +N+VS+IAH+P+DC SIPEGELNYPS
Sbjct: 601 HVNPSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPS 660
Query: 661 FMVKLGQVQTFSRTVTYVGSGREVYNVVIEA-PEGVSVTVRPRKVIFSALNQKATYSVTF 720
F V LG QTF+RTVT VG G EVY V+IEA P GVSVTVRPRK+ FSA+NQK YSVTF
Sbjct: 661 FSVALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTF 720
Query: 721 KRIGSIS-PSTEFAEGYLKWVSAKHLVRSPISVKFV 746
KRIGSI+ PS+ A+ YLK +S+KH+VRSPIS+KFV
Sbjct: 721 KRIGSITPPSSGSAQAYLKCMSSKHIVRSPISIKFV 750
BLAST of CsaV3_5G003440 vs. TAIR 10
Match:
AT1G04110.1 (Subtilase family protein )
HSP 1 Score: 587.4 bits (1513), Expect = 1.6e-167
Identity = 334/734 (45.50%), Postives = 455/734 (61.99%), Query Frame = 0
Query: 49 WHRSFLPTS---LENSEEQPT--LLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRET 108
WH SFL + +E EE+P+ LLYSY + + GF+A+LTE + + V+ R +
Sbjct: 48 WHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDH 107
Query: 109 IVHLHTTHSPNFLGLN--RQFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAK 168
++ + TT+S FLGL+ G W S FG+G IIGVLD G+ P PSF D GMP P K
Sbjct: 108 VLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRK 167
Query: 169 WKGRCE----FNFSACNNKLIGARSLNLASQALKGKITTLDDSP---------IDEDGHG 228
WKG C+ F+ S+CN KLIGAR + ++SP D GHG
Sbjct: 168 WKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSP----EESPNMPREYISARDSTGHG 227
Query: 229 THTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGESCSNVDILAGLDAAVED 288
THTAST G+ V A LGN G A GMAP AH+A+YKVC+ C + DILA +D A++D
Sbjct: 228 THTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQD 287
Query: 289 GVDVLSISLGGPPVPFFADITAIGAFAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVA 348
VDVLS+SLGG P+P + D AIG F A+++GI V C+A N+GP ++++N APW+ T+
Sbjct: 288 KVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIG 347
Query: 349 ASTIDRKITATAKLGNGEEFDGESLFQ----PNDFPQTFLPLVFPGEKNETVALCAEGSL 408
A T+DR+ A +L NG+ GESL+ N + + V G+K C GSL
Sbjct: 348 AGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEVIYVTGGDKGS--EFCLRGSL 407
Query: 409 KNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILLNAESDGFTTEADAHVLPASHVS 468
+++GK+V+CDRG R KG VK AGG AMIL N E + D H+LPA+ +
Sbjct: 408 PREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIG 467
Query: 469 HTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAIAAFSSRGPSLASPGILKPDITGPG 528
+T ++ +KAY+N+T P A I+F GT IG +P +A FS+RGPSLA+P ILKPD+ PG
Sbjct: 468 YTESVLLKAYVNATVKPKARIIFGGTVIGRSRAPEVAQFSARGPSLANPSILKPDMIAPG 527
Query: 529 VSILAAWP-------FPLDNNTNTKSTFNIVSGTSMSCPHLSGIAALIKSAHPDWSPAAI 588
V+I+AAWP P D+ + F ++SGTSMSCPH+SGI ALI+SA+P+WSPAAI
Sbjct: 528 VNIIAAWPQNLGPTGLPYDSR---RVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAI 587
Query: 589 KSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVDPGLVYDIQPDDYIPYLC 648
KS++MTTA++ + +G I D +PA +FAIGAGHVNP KA++PGLVY+IQP DYI YLC
Sbjct: 588 KSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLC 647
Query: 649 GLGYTNNQVSLIAHKPIDCL-TTTSIPEGELNYPSFMV--KLGQ-VQTFSRTVTYVGSGR 708
LG+T + + I HK + C P LNYPS V K G+ + +R VT VGS
Sbjct: 648 TLGFTRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPN 707
Query: 709 EVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTF--KRIGSISPSTEFAEGYLKWVS 743
+Y+V ++APEG+ V V P++++F ++Q +Y V F K+ FA+G L WV+
Sbjct: 708 SIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKKNRGGKVASFAQGQLTWVN 767
BLAST of CsaV3_5G003440 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 576.2 bits (1484), Expect = 3.7e-164
Identity = 337/758 (44.46%), Postives = 465/758 (61.35%), Query Frame = 0
Query: 6 SLFLLLLLNF-HVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLENSEEQ 65
+ FLLL L F HV + S+ TYIVH+ K ++ + H ++ +SL + +
Sbjct: 10 AFFLLLCLGFCHVSSSS------SDQGTYIVHMAKSQMPSSFD-LHSNWYDSSLRSISDS 69
Query: 66 PTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNRQFG- 125
LLY+Y N + GFS RLT+E ++ + G +S E LHTT +P FLGL+
Sbjct: 70 AELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD 129
Query: 126 FWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCE----FNFSACNNKLIG 185
+ ++ V++GVLD G+ P S+ D G P+ WKG CE F S CN KLIG
Sbjct: 130 LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIG 189
Query: 186 ARSLNLASQALKGKITTLDD--SPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMA 245
AR ++ G I + SP D+DGHGTHT+STAAG+ V+GA LG A GTA GMA
Sbjct: 190 ARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 249
Query: 246 PLAHLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAI 305
P A +A+YKVC+ C + DILA +D A+ D V+VLS+SLGG ++ D AIGAFAA+
Sbjct: 250 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAM 309
Query: 306 QKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPN 365
++GI VSCSA N+GP +++LSN APWI TV A T+DR A A LGNG+ F G SLF+
Sbjct: 310 ERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGE 369
Query: 366 DFPQTFLPLVFPGEKNETV--ALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAG 425
P LP ++ G + LC G+L VKGK+V+CDRG AR+ KG VK AG
Sbjct: 370 ALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAG 429
Query: 426 GAAMILLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDD 485
G MIL N ++G ADAH+LPA+ V A I+ Y+ + PTA+I GT +G
Sbjct: 430 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 489
Query: 486 FSPAIAAFSSRGPSLASPGILKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNIVSG 545
SP +AAFSSRGP+ +P ILKPD+ PGV+ILAAW P L +++ + FNI+SG
Sbjct: 490 PSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR-RVEFNIISG 549
Query: 546 TSMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVD-QTLQPADLFAIG 605
TSMSCPH+SG+AAL+KS HP+WSPAAI+S++MTTA T +G P++D T +P+ F G
Sbjct: 550 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 609
Query: 606 AGHVNPSKAVDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNY 665
AGHV+P+ A +PGL+YD+ +DY+ +LC L YT+ Q+ ++ + C + S +LNY
Sbjct: 610 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNY 669
Query: 666 PSFMVKLGQV--QTFSRTVTYVGSGREVYNV-VIEAPEGVSVTVRPRKVIFSALNQKATY 725
PSF V + V ++RTVT VG G Y+V V GV ++V P + F N+K +Y
Sbjct: 670 PSFAVNVDGVGAYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSY 729
Query: 726 SVTFKRIGSISPSTEFAEGYLKWVSAKHLVRSPISVKF 745
+VTF + S PS + G ++W KH+V SP+++ +
Sbjct: 730 TVTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756
BLAST of CsaV3_5G003440 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 573.2 bits (1476), Expect = 3.1e-163
Identity = 342/751 (45.54%), Postives = 448/751 (59.65%), Query Frame = 0
Query: 28 SNLHTYIVHV---KKPEVVDDLESWHRSFLPTSLENSEEQPTLLYSYRNVMSGFSARLTE 87
SN TYIVHV KP + W+ S L SL +S P+++++Y V GFSARLT
Sbjct: 23 SNSLTYIVHVDHEAKPSIFPTHFHWYTSSL-ASLTSS--PPSIIHTYDTVFHGFSARLTS 82
Query: 88 EHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR--QFGFWKDSNFGKGVIIGVLDGG 147
+ + + +S E + HLHTT SP FLGL + G ++S+FG ++IGV+D G
Sbjct: 83 QDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTG 142
Query: 148 ITPSHPSFVDAGMPQPPAKWKGRC----EFNFSACNNKLIGARSLNLASQALKGKI--TT 207
+ P PSF D G+ P KWKG+C +F SACN KL+GAR +A GK+ TT
Sbjct: 143 VWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETT 202
Query: 208 LDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGESCSNV 267
SP D DGHGTHTAS +AG +V A LG A G A GMAP A LA YKVC+ C +
Sbjct: 203 EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDS 262
Query: 268 DILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKGIFVSCSAANSGPFNAT 327
DILA D AV DGVDV+S+S+GG VP++ D AIGAF AI +GIFVS SA N GP T
Sbjct: 263 DILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALT 322
Query: 328 LSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLF-QPNDFPQTFLPLVFPGE---- 387
++N APW+ TV A TIDR A KLGNG+ G S++ P P PLV+ G
Sbjct: 323 VTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGG 382
Query: 388 KNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILLNAESDGFTT 447
+ +LC EGSL VKGK+V+CDRG +R KG V+ GG MI+ N DG
Sbjct: 383 DGYSSSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKGEIVRKNGGLGMIIANGVFDGEGL 442
Query: 448 EADAHVLPASHVSHTAALKIKAYIN------STTYPTATIVFKGTTIGDDFSPAIAAFSS 507
AD HVLPA+ V + +I+ YI+ S+ +PTATIVFKGT +G +P +A+FS+
Sbjct: 443 VADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSA 502
Query: 508 RGPSLASPGILKPDITGPGVSILAAWPFPLD----NNTNTKSTFNIVSGTSMSCPHLSGI 567
RGP+ +P ILKPD+ PG++ILAAWP + + N ++ FNI+SGTSM+CPH+SG+
Sbjct: 503 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 562
Query: 568 AALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQ-TLQPADLFAIGAGHVNPSKAVD 627
AAL+K+AHPDWSPAAI+S+++TTA + G P++D+ T + + G+GHV+P+KA+D
Sbjct: 563 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 622
Query: 628 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPE-GELNYPSFMVKLGQV 687
PGLVYDI DYI +LC YT + I + DC G LNYPSF V Q
Sbjct: 623 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQY 682
Query: 688 ------QTFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRI 743
F RTVT VG VY + I P G +VTV P K+ F + QK ++ V K
Sbjct: 683 GESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTT 742
BLAST of CsaV3_5G003440 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 568.5 bits (1464), Expect = 7.7e-162
Identity = 340/748 (45.45%), Postives = 448/748 (59.89%), Query Frame = 0
Query: 30 LHTYIVHVK---KPEVVDDLESWHRSFLPTSLENSEEQPTLLYSYRNVMSGFSARLTEEH 89
L +YIVHV+ KP + +WH S L SL +S + TLLYSY + GFSARL+
Sbjct: 30 LESYIVHVQRSHKPSLFSSHNNWHVSLL-RSLPSSPQPATLLYSYSRAVHGFSARLSPIQ 89
Query: 90 VKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPS 149
A+ +S + +HTTH+P FLG ++ G W +SN+G+ VI+GVLD GI P
Sbjct: 90 TAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPE 149
Query: 150 HPSFVDAGMPQPPAKWKGRCE----FNFSACNNKLIGARSLN----LASQALKGKITTLD 209
HPSF D+G+ P+ WKG CE F S+CN KLIGAR+ K
Sbjct: 150 HPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKES 209
Query: 210 DSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGESCSNVDI 269
SP D +GHGTHTASTAAG+ V A A GTA GMA A +A YK+C+ C + DI
Sbjct: 210 RSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTGGCYDSDI 269
Query: 270 LAGLDAAVEDGVDVLSISLG--GPPVPFFADITAIGAFAAIQKGIFVSCSAANSGPFNAT 329
LA +D AV DGV V+S+S+G G + D AIGAF A + GI VSCSA NSGP T
Sbjct: 270 LAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPET 329
Query: 330 LSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKNETV 389
+N APWILTV AST+DR+ A A G+G+ F G SL+ P + L LV+ G+
Sbjct: 330 ATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSGDCGS-- 389
Query: 390 ALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILLNAESDGFTTEADAH 449
LC G L + V+GK+V+CDRGG AR+ KG VK AGGA MIL N G AD+H
Sbjct: 390 RLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAESGEELTADSH 449
Query: 450 VLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIG-DDFSPAIAAFSSRGPSLASPGI 509
++PA+ V A +I+ YI ++ PTA I F GT IG SP +AAFSSRGP+ +P I
Sbjct: 450 LVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVI 509
Query: 510 LKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNIVSGTSMSCPHLSGIAALIKSAHP 569
LKPD+ PGV+ILA W P LD + + FNI+SGTSMSCPH+SG+AAL++ AHP
Sbjct: 510 LKPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCPHVSGLAALLRKAHP 569
Query: 570 DWSPAAIKSSIMTTANITNLEGNPIVD-QTLQPADLFAIGAGHVNPSKAVDPGLVYDIQP 629
DWSPAAIKS+++TTA G PI D T + ++ F GAGHV+P+KA++PGLVYDI+
Sbjct: 570 DWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEV 629
Query: 630 DDYIPYLCGLGYTNNQVSLIAHKPI---DCLTTTSIPEGELNYPSFMV---KLGQVQTFS 689
+Y+ +LC +GY + + P C T+ G+LNYPSF V G+V +
Sbjct: 630 KEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYK 689
Query: 690 RTVTYVGSGRE-VYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKR------IGSI 745
R V VGS + VY V +++P V + V P K+ FS Y VTFK +GS+
Sbjct: 690 RVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSV 749
BLAST of CsaV3_5G003440 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 566.6 bits (1459), Expect = 2.9e-161
Identity = 330/745 (44.30%), Postives = 448/745 (60.13%), Query Frame = 0
Query: 22 VTELPFSNLH-----TYIV---HVKKPEVVDDLESWHRSFLPTSLENSEEQPTLLYSYRN 81
+T F LH TYI+ H KPE W+ TS NSE +LLY+Y
Sbjct: 14 ITTFLFLLLHTTAKKTYIIRVNHSDKPESFLTHHDWY-----TSQLNSES--SLLYTYTT 73
Query: 82 VMSGFSARL-TEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNRQFGFWKDSNFGK 141
GFSA L + E + + + + + LHTT +P FLGLN +FG +
Sbjct: 74 SFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSN 133
Query: 142 GVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCE----FNFSACNNKLIGARSLNLASQ 201
GVIIGVLD G+ P SF D MP+ P+KWKG CE F+ CN KLIGARS + Q
Sbjct: 134 GVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQ 193
Query: 202 ALKG---KITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIY 261
G SP D DGHGTHT++TAAG+ V A LG A GTA GMA A +A Y
Sbjct: 194 MASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATY 253
Query: 262 KVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKGIFVSC 321
KVC+ C DILA +D A+ DGVDVLS+SLGG P++ D AIGAF+A+++G+FVSC
Sbjct: 254 KVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSC 313
Query: 322 SAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTFLP 381
SA NSGP A+++N APW++TV A T+DR A A LGNG+ G SL+ L
Sbjct: 314 SAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLE 373
Query: 382 LVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILLNAE 441
LV+ + + LC GSL + V+GK+VVCDRG AR+ KG V++AGG MI+ N
Sbjct: 374 LVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTA 433
Query: 442 SDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAIAAFSS 501
+ G AD+H+LPA V ++ Y+ S + PTA +VFKGT + SP +AAFSS
Sbjct: 434 ASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSS 493
Query: 502 RGPSLASPGILKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNIVSGTSMSCPHLSG 561
RGP+ +P ILKPD+ GPGV+ILA W P LD ++ ++ FNI+SGTSMSCPH+SG
Sbjct: 494 RGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSR-RTQFNIMSGTSMSCPHISG 553
Query: 562 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQP-ADLFAIGAGHVNPSKAV 621
+A L+K+AHP+WSP+AIKS++MTTA + + P+ D ++ +A G+GHV+P KA+
Sbjct: 554 LAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKAL 613
Query: 622 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKP-IDCLTTTSIPEGELNYPSFMVKLG- 681
PGLVYDI ++YI +LC L YT + + I +P ++C S P G+LNYPSF V G
Sbjct: 614 SPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDP-GQLNYPSFSVLFGG 673
Query: 682 -QVQTFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSI 741
+V ++R VT VG+ VY V + V ++V+P K+ F ++ +K Y+VTF +
Sbjct: 674 KRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGV 733
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| KGN50165.1 | 0.0e+00 | 100.00 | hypothetical protein Csa_000664 [Cucumis sativus] | [more] |
| KAA0042818.1 | 0.0e+00 | 95.70 | subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa] | [more] |
| XP_008437181.1 | 0.0e+00 | 95.44 | PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo] | [more] |
| XP_038876087.1 | 0.0e+00 | 88.45 | LOW QUALITY PROTEIN: subtilisin-like protease 4 [Benincasa hispida] | [more] |
| XP_023532974.1 | 0.0e+00 | 82.42 | subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A9QY38 | 1.0e-283 | 65.56 | Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1 | [more] |
| A0A0M3R8G2 | 1.1e-248 | 59.97 | Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1 | [more] |
| A9QY40 | 5.2e-240 | 60.49 | Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1 | [more] |
| A9QY39 | 1.6e-233 | 57.50 | Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1 | [more] |
| G7KEU7 | 2.7e-228 | 57.01 | Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KKE3 | 0.0e+00 | 100.00 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1 | [more] |
| A0A5A7TJ55 | 0.0e+00 | 95.70 | Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
| A0A1S3ATZ7 | 0.0e+00 | 95.44 | subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=... | [more] |
| A0A6J1H242 | 0.0e+00 | 82.42 | subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE... | [more] |
| A0A6J1E0E3 | 0.0e+00 | 79.63 | subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 P... | [more] |
