CsaV3_4G036250 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_4G036250
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	Threonyl-tRNA synthetase
Location	chr4: 25479117 .. 25479451 (-)
RNA-Seq Expression	CsaV3_4G036250
Synteny	CsaV3_4G036250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTGCTATGATTTTGATATAGAGCCCTTGGGAGATAAAGATTTGATGAAAATCAAGAAGGAGATGGTTGGTATTATACCAAATCAATGAGCATTGAAGTCACTGGTTTTCTTTTTCTTTATTATTTTACATCACATGATTTGGAATATATGCATTAGTCTATGCTTTTAGTATGTGGGGTTTATTTTTCATATCTTCTCTAGGAACGCATTATCAGCACAAACTTACCACTTGTAAGAGAAGAAGTTTCAAGGGATAAAGCTTAA

mRNA sequence

ATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTGCTATGATTTTGATATAGAGCCCTTGGGAGATAAAGATTTGATGAAAATCAAGAAGGAGATGGAACGCATTATCAGCACAAACTTACCACTTGTAAGAGAAGAAGTTTCAAGGGATAAAGCTTAA

Coding sequence (CDS)

ATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTGCTATGATTTTGATATAGAGCCCTTGGGAGATAAAGATTTGATGAAAATCAAGAAGGAGATGGAACGCATTATCAGCACAAACTTACCACTTGTAAGAGAAGAAGTTTCAAGGGATAAAGCTTAA

Protein sequence

MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEVSRDKA*

Homology

[Close]

BLAST of CsaV3_4G036250 vs. NCBI nr
Match: KAE8649928.1 (hypothetical protein Csa_012436 [Cucumis sativus])

HSP 1 Score: 137.1 bits (344), Expect = 5.4e-29
Identity = 65/65 (100.00%), Postives = 65/65 (100.00%), Query Frame = 0


Query: 1  MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
          MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV
Sbjct: 1  MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60

Query: 61 SRDKA 66
          SRDKA
Sbjct: 61 SRDKA 65

[Close]

BLAST of CsaV3_4G036250 vs. NCBI nr
Match: KAG7011877.1 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 125.6 bits (314), Expect = 1.6e-25
Identity = 60/65 (92.31%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLVREEV
Sbjct: 112 MAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEV 171

Query: 61  SRDKA 66
           SRD+A
Sbjct: 172 SRDEA 176

[Close]

BLAST of CsaV3_4G036250 vs. NCBI nr
Match: KAA0059181.1 (threonine--tRNA ligase [Cucumis melo var. makuwa] >TYK19338.1 threonine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 125.6 bits (314), Expect = 1.6e-25
Identity = 60/65 (92.31%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLVREEV
Sbjct: 112 MAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEV 171

Query: 61  SRDKA 66
           SRD+A
Sbjct: 172 SRDEA 176

[Close]

BLAST of CsaV3_4G036250 vs. NCBI nr
Match: XP_004144536.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucumis sativus] >KGN43435.1 hypothetical protein Csa_020587 [Cucumis sativus])

HSP 1 Score: 125.6 bits (314), Expect = 1.6e-25
Identity = 60/65 (92.31%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLVREEV
Sbjct: 112 MAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEV 171

Query: 61  SRDKA 66
           SRD+A
Sbjct: 172 SRDEA 176

[Close]

BLAST of CsaV3_4G036250 vs. NCBI nr
Match: XP_022989354.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita maxima] >XP_022989355.1 threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita maxima])

HSP 1 Score: 125.6 bits (314), Expect = 1.6e-25
Identity = 60/65 (92.31%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLVREEV
Sbjct: 112 MAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEV 171

Query: 61  SRDKA 66
           SRD+A
Sbjct: 172 SRDEA 176

[Close]

BLAST of CsaV3_4G036250 vs. ExPASy Swiss-Prot
Match: F4IFC5 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2761 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 1.3e-25
Identity = 54/65 (83.08%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWI+NGF YDFD+EPL DKDL +IKKEM+RIIS NLPL+REEV
Sbjct: 90  MAMAVQKLFPDAKVTIGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEV 149

Query: 61  SRDKA 66
           SR++A
Sbjct: 150 SREEA 154

[Close]

BLAST of CsaV3_4G036250 vs. ExPASy Swiss-Prot
Match: Q8DGW0 (Threonine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=thrS PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.0e-18
Identity = 44/66 (66.67%), Postives = 54/66 (81.82%), Query Frame = 0


Query: 1  MAMAVQKLFPDAKVTIGPWIENGFCYDFD-IEPLGDKDLMKIKKEMERIISTNLPLVREE 60
          +AMAVQKLFP A+VTIGPWIENGF YDFD  EP  +KDL  I+KEM +II   LP++REE
Sbjct: 31 LAMAVQKLFPKAQVTIGPWIENGFYYDFDHPEPFSEKDLRLIEKEMVKIIKRKLPVIREE 90

Query: 61 VSRDKA 66
          V+R++A
Sbjct: 91 VTREEA 96

[Close]

BLAST of CsaV3_4G036250 vs. ExPASy Swiss-Prot
Match: Q55806 (Threonine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=thrS PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.4e-18
Identity = 43/66 (65.15%), Postives = 53/66 (80.30%), Query Frame = 0


Query: 1  MAMAVQKLFPDAKVTIGPWIENGFCYDFDI-EPLGDKDLMKIKKEMERIISTNLPLVREE 60
          MAMAVQKLFP A+VTIGPW E GF YDFD+ EP  + DL  IKKEM +II+  LP++REE
Sbjct: 33 MAMAVQKLFPKAQVTIGPWTETGFYYDFDVAEPFTEADLKAIKKEMVKIINKKLPVIREE 92

Query: 61 VSRDKA 66
          +SR++A
Sbjct: 93 ISREEA 98

[Close]

BLAST of CsaV3_4G036250 vs. ExPASy Swiss-Prot
Match: Q31JY3 (Threonine--tRNA ligase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=thrS PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 7.6e-18
Identity = 45/66 (68.18%), Postives = 51/66 (77.27%), Query Frame = 0


Query: 1  MAMAVQKLFPDAKVTIGPWIENGFCYDFDI-EPLGDKDLMKIKKEMERIISTNLPLVREE 60
          MAMAVQKLFP A+VTIGPW E GF YDFD  EP  + DL  IKKEM +II   LP+VREE
Sbjct: 33 MAMAVQKLFPKAQVTIGPWTETGFYYDFDTPEPFTEADLKAIKKEMVKIIQQKLPVVREE 92

Query: 61 VSRDKA 66
          VSR++A
Sbjct: 93 VSREEA 98

[Close]

BLAST of CsaV3_4G036250 vs. ExPASy Swiss-Prot
Match: Q5N1X6 (Threonine--tRNA ligase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=thrS PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 7.6e-18
Identity = 45/66 (68.18%), Postives = 51/66 (77.27%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDI-EPLGDKDLMKIKKEMERIISTNLPLVREE 60
           MAMAVQKLFP A+VTIGPW E GF YDFD  EP  + DL  IKKEM +II   LP+VREE
Sbjct: 36  MAMAVQKLFPKAQVTIGPWTETGFYYDFDTPEPFTEADLKAIKKEMVKIIQQKLPVVREE 95

Query: 61  VSRDKA 66
           VSR++A
Sbjct: 96  VSREEA 101

[Close]

BLAST of CsaV3_4G036250 vs. ExPASy TrEMBL
Match: A0A0A0L0Y5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G650170 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.6e-29
Identity = 65/65 (100.00%), Postives = 65/65 (100.00%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV
Sbjct: 60  MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 119

Query: 61  SRDKA 66
           SRDKA
Sbjct: 120 SRDKA 124

[Close]

BLAST of CsaV3_4G036250 vs. ExPASy TrEMBL
Match: A0A6J1JP39 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111486433 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.9e-26
Identity = 60/65 (92.31%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLVREEV
Sbjct: 112 MAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEV 171

Query: 61  SRDKA 66
           SRD+A
Sbjct: 172 SRDEA 176

[Close]

BLAST of CsaV3_4G036250 vs. ExPASy TrEMBL
Match: A0A5A7V0A9 (Threonyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00590 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.9e-26
Identity = 60/65 (92.31%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLVREEV
Sbjct: 112 MAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEV 171

Query: 61  SRDKA 66
           SRD+A
Sbjct: 172 SRDEA 176

[Close]

BLAST of CsaV3_4G036250 vs. ExPASy TrEMBL
Match: A0A0A0K179 (Threonyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_7G033430 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.9e-26
Identity = 60/65 (92.31%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLVREEV
Sbjct: 112 MAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEV 171

Query: 61  SRDKA 66
           SRD+A
Sbjct: 172 SRDEA 176

[Close]

BLAST of CsaV3_4G036250 vs. ExPASy TrEMBL
Match: A0A6J1GM45 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.9e-26
Identity = 60/65 (92.31%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLVREEV
Sbjct: 112 MAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEV 171

Query: 61  SRDKA 66
           SRD+A
Sbjct: 172 SRDEA 176

[Close]

BLAST of CsaV3_4G036250 vs. TAIR 10
Match: AT2G04842.1 (threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative )

HSP 1 Score: 116.3 bits (290), Expect = 9.2e-27
Identity = 54/65 (83.08%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 1   MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60
           MAMAVQKLFPDAKVTIGPWI+NGF YDFD+EPL DKDL +IKKEM+RIIS NLPL+REEV
Sbjct: 90  MAMAVQKLFPDAKVTIGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEV 149

Query: 61  SRDKA 66
           SR++A
Sbjct: 150 SREEA 154

The following BLAST results are available for this feature:

BLAST of CsaV3_4G036250 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8649928.1	5.4e-29	100.00	hypothetical protein Csa_012436 [Cucumis sativus]	[more]
KAG7011877.1	1.6e-25	92.31	Threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita argyrosperma su...	[more]
KAA0059181.1	1.6e-25	92.31	threonine--tRNA ligase [Cucumis melo var. makuwa] >TYK19338.1 threonine--tRNA li...	[more]
XP_004144536.1	1.6e-25	92.31	threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucumis sativus] >KGN4343...	[more]
XP_022989354.1	1.6e-25	92.31	threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita maxima] >X...	[more]

BLAST of CsaV3_4G036250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IFC5	1.3e-25	83.08	Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX...	[more]
Q8DGW0	2.0e-18	66.67	Threonine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 ...	[more]
Q55806	3.4e-18	65.15	Threonine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=111170...	[more]
Q31JY3	7.6e-18	68.18	Threonine--tRNA ligase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) ...	[more]
Q5N1X6	7.6e-18	68.18	Threonine--tRNA ligase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG...	[more]

BLAST of CsaV3_4G036250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0Y5	2.6e-29	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G650170 PE=4 SV=1	[more]
A0A6J1JP39	7.9e-26	92.31	Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111486433 PE=3 SV=1	[more]
A0A5A7V0A9	7.9e-26	92.31	Threonyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A0A0K179	7.9e-26	92.31	Threonyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_7G033430 PE=3 SV=1	[more]
A0A6J1GM45	7.9e-26	92.31	Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1	[more]

BLAST of CsaV3_4G036250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04842.1	9.2e-27	83.08	threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.980.10		coord: 1..65 e-value: 1.9E-18 score: 68.9
None	No IPR available	PANTHER	PTHR11451:SF44	THREONINE--TRNA LIGASE, CHLOROPLASTIC/MITOCHONDRIAL 2	coord: 1..65
IPR002320	Threonine-tRNA ligase, class IIa	PANTHER	PTHR11451	THREONINE-TRNA LIGASE	coord: 1..65
IPR018163	Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily	SUPERFAMILY	55186	ThrRS/AlaRS common domain	coord: 1..65

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_4G036250.1	CsaV3_4G036250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006435	threonyl-tRNA aminoacylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0004829	threonine-tRNA ligase activity