CsaV3_4G032030 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_4G032030 vs. NCBI nr
Match: XP_011653984.1 (TLC domain-containing protein 4 [Cucumis sativus] >KGN55433.2 hypothetical protein Csa_012466 [Cucumis sativus])

HSP 1 Score: 506.9 bits (1304), Expect = 1.0e-139
Identity = 263/263 (100.00%), Postives = 263/263 (100.00%), Query Frame = 0

Query: 1   MSLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRV 60
           MSLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRV
Sbjct: 1   MSLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRV 60

Query: 61  EWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDL 120
           EWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDL
Sbjct: 61  EWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDL 120

Query: 121 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 180
           VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT
Sbjct: 121 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 180

Query: 181 AGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSIL 240
           AGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSIL
Sbjct: 181 AGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSIL 240

Query: 241 WIMNLTWFSKIFRGLKKTLAKRQ 264
           WIMNLTWFSKIFRGLKKTLAKRQ
Sbjct: 241 WIMNLTWFSKIFRGLKKTLAKRQ 263

BLAST of CsaV3_4G032030 vs. NCBI nr
Match: XP_008466799.1 (PREDICTED: transmembrane protein 56-like [Cucumis melo] >XP_008466800.1 PREDICTED: transmembrane protein 56-like [Cucumis melo])

HSP 1 Score: 485.3 bits (1248), Expect = 3.3e-133
Identity = 253/263 (96.20%), Postives = 258/263 (98.10%), Query Frame = 0

Query: 1   MSLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRV 60
           MSLKSQA +LLKYLVLSDPVVPYISIPAGVLASKLVYDLTD+IS VYFKSYSNLSRIQRV
Sbjct: 1   MSLKSQAEQLLKYLVLSDPVVPYISIPAGVLASKLVYDLTDLISGVYFKSYSNLSRIQRV 60

Query: 61  EWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDL 120
           EWNNRAMSTVHAIFITAAALYMVFLSDLYSD+YFGPITFR+STISTFGLGVSVGYFITDL
Sbjct: 61  EWNNRAMSTVHAIFITAAALYMVFLSDLYSDRYFGPITFRSSTISTFGLGVSVGYFITDL 120

Query: 121 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 180
           VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT
Sbjct: 121 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 180

Query: 181 AGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSIL 240
           AGMKKSKAYLINGIVIFFAWLVARILLFMY+FYHVYLHLNQVLQMHVFGQ LVFVVP IL
Sbjct: 181 AGMKKSKAYLINGIVIFFAWLVARILLFMYLFYHVYLHLNQVLQMHVFGQLLVFVVPLIL 240

Query: 241 WIMNLTWFSKIFRGLKKTLAKRQ 264
            IMNLTWFSKIFRGLKKTLAKRQ
Sbjct: 241 SIMNLTWFSKIFRGLKKTLAKRQ 263

BLAST of CsaV3_4G032030 vs. NCBI nr
Match: XP_038899443.1 (LOW QUALITY PROTEIN: TLC domain-containing protein 4-like [Benincasa hispida])

HSP 1 Score: 472.2 bits (1214), Expect = 2.8e-129
Identity = 243/263 (92.40%), Postives = 255/263 (96.96%), Query Frame = 0

Query: 1   MSLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRV 60
           MSLKSQA +LLKYLVLSDP VPYISIPAG+LASKLVYDLTD+IS VYFKSYSNLSRIQRV
Sbjct: 1   MSLKSQAEQLLKYLVLSDPAVPYISIPAGILASKLVYDLTDLISGVYFKSYSNLSRIQRV 60

Query: 61  EWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDL 120
           EWNNRAMSTVHA+FITAAALYMVFLSDLYSD+YF  +TFR+STISTFGLGVSVGYFITDL
Sbjct: 61  EWNNRAMSTVHAVFITAAALYMVFLSDLYSDRYFPLVTFRSSTISTFGLGVSVGYFITDL 120

Query: 121 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 180
            MILWFYPAIGGMEYVIHHLLSL+AVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT
Sbjct: 121 GMILWFYPAIGGMEYVIHHLLSLVAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 180

Query: 181 AGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSIL 240
           AGMKKSKAYLING+VIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVP IL
Sbjct: 181 AGMKKSKAYLINGVVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPLIL 240

Query: 241 WIMNLTWFSKIFRGLKKTLAKRQ 264
            +MNLTWF+KIF+GLKKTLAKRQ
Sbjct: 241 SVMNLTWFAKIFKGLKKTLAKRQ 263

BLAST of CsaV3_4G032030 vs. NCBI nr
Match: XP_022940088.1 (transmembrane protein 56-like [Cucurbita moschata] >XP_022940089.1 transmembrane protein 56-like [Cucurbita moschata] >XP_022940090.1 transmembrane protein 56-like [Cucurbita moschata] >XP_022940091.1 transmembrane protein 56-like [Cucurbita moschata] >XP_022940092.1 transmembrane protein 56-like [Cucurbita moschata] >XP_023525630.1 transmembrane protein 56-like [Cucurbita pepo subsp. pepo] >XP_023525631.1 transmembrane protein 56-like [Cucurbita pepo subsp. pepo] >XP_023525633.1 transmembrane protein 56-like [Cucurbita pepo subsp. pepo] >XP_023525634.1 transmembrane protein 56-like [Cucurbita pepo subsp. pepo] >XP_023525635.1 transmembrane protein 56-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.8 bits (1166), Expect = 1.0e-123
Identity = 233/262 (88.93%), Postives = 250/262 (95.42%), Query Frame = 0

Query: 2   SLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRVE 61
           S +SQA +LLKYLVLSDPVVPYISIPAG+LASKLVYDLTD+IS VYFKSYSNLSRIQR+E
Sbjct: 5   SYQSQAEQLLKYLVLSDPVVPYISIPAGILASKLVYDLTDLISGVYFKSYSNLSRIQRME 64

Query: 62  WNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDLV 121
           WNNRAMST+HAIFITAA+LYMVFLSDLYSD+YFG ITFR+STISTF LGVSVGYFITDL 
Sbjct: 65  WNNRAMSTLHAIFITAASLYMVFLSDLYSDRYFGFITFRSSTISTFCLGVSVGYFITDLG 124

Query: 122 MILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDTA 181
           MI WFYP++GGMEYVIHHLLSL+AVAYAMLTGEGQLYT+MVLISETTTPGINLRWYLDTA
Sbjct: 125 MISWFYPSLGGMEYVIHHLLSLVAVAYAMLTGEGQLYTFMVLISETTTPGINLRWYLDTA 184

Query: 182 GMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSILW 241
           GMKKSKAYLING+VIFFAWLVARILLFMYMFYHVYLHLNQVLQMH FGQ LVFVVP +L 
Sbjct: 185 GMKKSKAYLINGVVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHAFGQLLVFVVPLVLS 244

Query: 242 IMNLTWFSKIFRGLKKTLAKRQ 264
           +MNL WF+KIFRGLKKTLAKRQ
Sbjct: 245 VMNLMWFAKIFRGLKKTLAKRQ 266

BLAST of CsaV3_4G032030 vs. NCBI nr
Match: KAG6607986.1 (TLC domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 453.8 bits (1166), Expect = 1.0e-123
Identity = 233/262 (88.93%), Postives = 250/262 (95.42%), Query Frame = 0

Query: 2   SLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRVE 61
           S +SQA +LLKYLVLSDPVVPYISIPAG+LASKLVYDLTD+IS VYFKSYSNLSRIQR+E
Sbjct: 77  SYQSQAEQLLKYLVLSDPVVPYISIPAGILASKLVYDLTDLISGVYFKSYSNLSRIQRME 136

Query: 62  WNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDLV 121
           WNNRAMST+HAIFITAA+LYMVFLSDLYSD+YFG ITFR+STISTF LGVSVGYFITDL 
Sbjct: 137 WNNRAMSTLHAIFITAASLYMVFLSDLYSDRYFGFITFRSSTISTFCLGVSVGYFITDLG 196

Query: 122 MILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDTA 181
           MI WFYP++GGMEYVIHHLLSL+AVAYAMLTGEGQLYT+MVLISETTTPGINLRWYLDTA
Sbjct: 197 MISWFYPSLGGMEYVIHHLLSLVAVAYAMLTGEGQLYTFMVLISETTTPGINLRWYLDTA 256

Query: 182 GMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSILW 241
           GMKKSKAYLING+VIFFAWLVARILLFMYMFYHVYLHLNQVLQMH FGQ LVFVVP +L 
Sbjct: 257 GMKKSKAYLINGVVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHAFGQLLVFVVPLVLS 316

Query: 242 IMNLTWFSKIFRGLKKTLAKRQ 264
           +MNL WF+KIFRGLKKTLAKRQ
Sbjct: 317 VMNLMWFAKIFRGLKKTLAKRQ 338

BLAST of CsaV3_4G032030 vs. ExPASy Swiss-Prot
Match: Q8CGF5 (TLC domain-containing protein 4 OS=Mus musculus OX=10090 GN=Tlcd4 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.4e-17
Identity = 70/240 (29.17%), Postives = 124/240 (51.67%), Query Frame = 0

Query: 30  VLASKLVYDLTDMISSVYFKS-----YSNLSRIQRVEWNNRAMSTVHAIFITAAALYMVF 89
           V +S +V+ +     S +F +     Y++LS  +++EWN+R +ST H++ +    LY+ F
Sbjct: 25  VCSSFVVFQILFHFVSYWFSARVSSGYNSLSIDKKIEWNSRVVSTCHSLLVGIFGLYLFF 84

Query: 90  LSD-LYSDQYFGPITFRNSTISTFGLGVSVGYFITDLVMILWFYPAIGGMEYVIHHLLSL 149
             +   +D  +G  T+ N  I+T     + GY I+DL++IL+ +  IG   ++IHH   L
Sbjct: 85  FDEATITDPLWGDPTYVNINIAT-----ASGYLISDLLIILFNWKVIGDKFFIIHHCAGL 144

Query: 150 LAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDTAGMKK-SKAYLINGIVIFFAWLV 209
            A  + + TG         L++E ++P +N RW+ +     K SKA +INGI++   + +
Sbjct: 145 TAYYFVLTTGALAYIANFRLLAELSSPFVNQRWFFEALKYPKFSKANVINGILMTVVFFI 204

Query: 210 ARILLF--MYMFYHVYLHLNQVLQMHVFGQFLVFVVPSILWIMNLTWFSKIFRGLKKTLA 261
            RI+    MY F +        ++     Q +  V   IL +MN+ W  KI +G  K ++
Sbjct: 205 VRIISIPPMYFFLYSVYGTEPYIRFGFVIQSVWIVTCVILDVMNIMWMIKITKGCIKVIS 259

BLAST of CsaV3_4G032030 vs. ExPASy Swiss-Prot
Match: Q6PGS5 (TLC domain-containing protein 4-B OS=Xenopus laevis OX=8355 GN=tlcd4-b PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.6e-16
Identity = 68/249 (27.31%), Postives = 127/249 (51.00%), Query Frame = 0

Query: 19  PVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRVEWNNRAMSTVHAIFITAA 78
           P+  +IS+ +  +   L +  +  +S+     +  LS  Q++EWN+R +S+ HA+ +   
Sbjct: 6   PLTVFISVTSLAVFQFLFHVGSSWLSTRLTGGFHKLSARQKIEWNSRTVSSFHALVVGCF 65

Query: 79  ALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDLVMILWFYPAIGGMEYVIH 138
            LY++    +Y D       + +  +    + V+ GY I+DL++I++++  IG   +V H
Sbjct: 66  CLYIL----VYDDAVNADPVWGDPFMVKLNVAVTSGYLISDLLLIIYYWKEIGDKYFVTH 125

Query: 139 HLLSLLAVAYAMLTGEGQL--YTYMVLISETTTPGINLRWYLDTAGMKK-SKAYLINGIV 198
           HL +L A  Y +  GEG L  +    LI+E +TP +N RW+ +  G  K S   ++NG++
Sbjct: 126 HLAALYACYYVL--GEGMLPYFGNFRLIAEFSTPFVNQRWFFEVLGYSKYSLPNMVNGVL 185

Query: 199 IFFAWLVARILLFMYMFYHVYLHL-NQVLQMHVFGQFLVFVVPSI-LWIMNLTWFSKIFR 258
           +  ++ + RI +    +  V+     +       G    +++ S+ L IMN+ W  KI +
Sbjct: 186 MTISFFIVRIAVIPIYYGRVFSTFGTEAFHRLGLGAQCAWIISSVSLDIMNVMWMIKIAK 245

Query: 259 GLKKTLAKR 263
           G  K L  R
Sbjct: 246 GCYKVLYHR 248

BLAST of CsaV3_4G032030 vs. ExPASy Swiss-Prot
Match: Q96MV1 (TLC domain-containing protein 4 OS=Homo sapiens OX=9606 GN=TLCD4 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.7e-13
Identity = 65/232 (28.02%), Postives = 118/232 (50.86%), Query Frame = 0

Query: 35  LVYDLTDMISSVYFKSYSNLSRIQRVEWNNRAMSTVHAIFITAAALYMVFLSD--LYSDQ 94
           L Y ++   S+     +++LS  +++EWN+R +ST H++ +    LY +FL D    +D 
Sbjct: 22  LFYFVSYWFSAKVSPGFNSLSFKKKIEWNSRVVSTCHSLVVGIFGLY-IFLFDEATKADP 81

Query: 95  YFGPITFRNSTISTFGLGVSVGYFITDLVMILWFYPAIGGMEYVIHHLLSLLAVAYAMLT 154
            +G       +++   + ++ GY I+DL +I+ ++  IG   +++HH  SL A    +  
Sbjct: 82  LWG-----GPSLANVNIAIASGYLISDLSIIILYWKVIGDKFFIMHHCASLYAYYLVLKN 141

Query: 155 GEGQLYTYMVLISETTTPGINLRWYLDTAGMKK-SKAYLINGI---VIFFAWLVARILLF 214
           G         L++E ++P +N RW+ +     K SKA +INGI   V+FF   +A +L  
Sbjct: 142 GVLAYIGNFRLLAELSSPFVNQRWFFEALKYPKFSKAIVINGILMTVVFFIVRIASMLPH 201

Query: 215 MYMFYHVYLHLNQVLQMHVFGQFLVFVVPSILWIMNLTWFSKIFRGLKKTLA 261
               Y VY      +++ V  Q    +   +L +MN+ W  KI +G  K ++
Sbjct: 202 YGFMYSVY-GTEPYIRLGVLIQLSWVISCVVLDVMNVMWMIKISKGCIKVIS 246

BLAST of CsaV3_4G032030 vs. ExPASy Swiss-Prot
Match: Q6GLX2 (TLC domain-containing protein 4-A OS=Xenopus laevis OX=8355 GN=tlcd4-a PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.0e-12
Identity = 61/249 (24.50%), Postives = 120/249 (48.19%), Query Frame = 0

Query: 16  LSDPVVPYISIPAGVLASKLVYD-LTDMISSVYFKSYSNLSRIQRVEWNNRAMSTVHAIF 75
           + D ++ Y  +    L  +L++  ++  + + Y  +Y  LS  ++ EW++R +ST HA+ 
Sbjct: 1   MDDVLISYCVVTGSFLGFQLLFSAISPRLFTKYSSTYRQLSFGKQCEWDSRFVSTNHALI 60

Query: 76  ITAAALYMVFLSD-LYSDQYFGPITFRNSTISTFGLGVSVGYFITDLVMILWFYPAIGGM 135
           + +A LY++   D + +D  +G   +    ++     ++ GY + DL+++  F+  +   
Sbjct: 61  VGSACLYILAYDDAVNADPIWGDPFWVKMNVA-----ITCGYLVQDLLLLARFWKVMRDP 120

Query: 136 EYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDTAGMKKSK-AYLIN 195
             V HHL    +  Y +  G    +    LISE +TP +N RW+ D  G  +S    L+N
Sbjct: 121 YMVCHHLAVFYSYGYVLNRGVLPYFANFRLISELSTPFVNQRWFFDVIGKPRSSWPVLLN 180

Query: 196 GIVIFFAWLVARILLFMYMFYHVYLHLNQ--VLQMHVFGQFLVFVVPSILWIMNLTWFSK 255
           G+ +   + + RI +    +  V+        +++ +  Q    V   +L I+N+ W  K
Sbjct: 181 GLAMALVFFIVRIAVIPSYYSQVFATFGTEGYIRLGIGPQVAWIVSCVVLDILNVFWMYK 240

Query: 256 IFRGLKKTL 260
           I RG  K +
Sbjct: 241 IARGFYKVV 244

BLAST of CsaV3_4G032030 vs. ExPASy Swiss-Prot
Match: Q5XIY2 (TLC domain-containing protein 4-B OS=Danio rerio OX=7955 GN=tlcd4b PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.5e-11
Identity = 56/226 (24.78%), Postives = 114/226 (50.44%), Query Frame = 0

Query: 39  LTDMISSVYFKSYSNLSRIQRVEWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPIT 98
           ++ ++SS + + Y  L   +  +WN+R +STVHA+ +    LY+++  D  ++    P+ 
Sbjct: 23  VSPVLSSNFTQGYGKLPPNKLNDWNSRLVSTVHALIVGLFCLYILWYDDAVNED---PV- 82

Query: 99  FRNSTISTFGLGVSVGYFITDLVMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLY 158
           + +  +    + ++ GY   DL+++   +  +G + +V HHL +L A  Y +  G    +
Sbjct: 83  WGDPNLVKLNVAITCGYLFYDLLLLACNWSTMGDVFFVCHHLAALYAYGYVLTRGVLPYF 142

Query: 159 TYMVLISETTTPGINLRWYLDTAGMKKSKAYLI-NGIVIFFAWLVARILL----FMYMFY 218
               LISE +TP +N RW+ +     ++   ++ NGI +   + + RI +    +  +F 
Sbjct: 143 ANFRLISELSTPFVNQRWFFEALAYPRTHQLVVANGIAMAVVFFLVRIAVMPPYWAKVFG 202

Query: 219 HVYLHLNQVLQMHVFGQFLVFVVPSILWIMNLTWFSKIFRGLKKTL 260
            +Y    + L + +   +++  V   L I+N+ W  KI RG  K +
Sbjct: 203 IIYSPTFEKLGLAIQVAWIISCV--CLDILNIIWMYKIARGCYKVI 242

BLAST of CsaV3_4G032030 vs. ExPASy TrEMBL
Match: A0A1S3CS93 (transmembrane protein 56-like OS=Cucumis melo OX=3656 GN=LOC103504121 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.6e-133
Identity = 253/263 (96.20%), Postives = 258/263 (98.10%), Query Frame = 0

Query: 1   MSLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRV 60
           MSLKSQA +LLKYLVLSDPVVPYISIPAGVLASKLVYDLTD+IS VYFKSYSNLSRIQRV
Sbjct: 1   MSLKSQAEQLLKYLVLSDPVVPYISIPAGVLASKLVYDLTDLISGVYFKSYSNLSRIQRV 60

Query: 61  EWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDL 120
           EWNNRAMSTVHAIFITAAALYMVFLSDLYSD+YFGPITFR+STISTFGLGVSVGYFITDL
Sbjct: 61  EWNNRAMSTVHAIFITAAALYMVFLSDLYSDRYFGPITFRSSTISTFGLGVSVGYFITDL 120

Query: 121 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 180
           VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT
Sbjct: 121 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 180

Query: 181 AGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSIL 240
           AGMKKSKAYLINGIVIFFAWLVARILLFMY+FYHVYLHLNQVLQMHVFGQ LVFVVP IL
Sbjct: 181 AGMKKSKAYLINGIVIFFAWLVARILLFMYLFYHVYLHLNQVLQMHVFGQLLVFVVPLIL 240

Query: 241 WIMNLTWFSKIFRGLKKTLAKRQ 264
            IMNLTWFSKIFRGLKKTLAKRQ
Sbjct: 241 SIMNLTWFSKIFRGLKKTLAKRQ 263

BLAST of CsaV3_4G032030 vs. ExPASy TrEMBL
Match: A0A0A0L1M1 (TLC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G614180 PE=4 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.2e-125
Identity = 243/263 (92.40%), Postives = 243/263 (92.40%), Query Frame = 0

Query: 1   MSLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRV 60
           MSLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRV
Sbjct: 1   MSLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRV 60

Query: 61  EWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDL 120
           EWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDL
Sbjct: 61  EWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDL 120

Query: 121 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 180
           VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT
Sbjct: 121 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 180

Query: 181 AGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSIL 240
           AGMKKSKAYLINGIVIFFAWL                    VLQMHVFGQFLVFVVPSIL
Sbjct: 181 AGMKKSKAYLINGIVIFFAWL--------------------VLQMHVFGQFLVFVVPSIL 240

Query: 241 WIMNLTWFSKIFRGLKKTLAKRQ 264
           WIMNLTWFSKIFRGLKKTLAKRQ
Sbjct: 241 WIMNLTWFSKIFRGLKKTLAKRQ 243

BLAST of CsaV3_4G032030 vs. ExPASy TrEMBL
Match: A0A6J1FJ18 (transmembrane protein 56-like OS=Cucurbita moschata OX=3662 GN=LOC111445812 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 5.1e-124
Identity = 233/262 (88.93%), Postives = 250/262 (95.42%), Query Frame = 0

Query: 2   SLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRVE 61
           S +SQA +LLKYLVLSDPVVPYISIPAG+LASKLVYDLTD+IS VYFKSYSNLSRIQR+E
Sbjct: 5   SYQSQAEQLLKYLVLSDPVVPYISIPAGILASKLVYDLTDLISGVYFKSYSNLSRIQRME 64

Query: 62  WNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDLV 121
           WNNRAMST+HAIFITAA+LYMVFLSDLYSD+YFG ITFR+STISTF LGVSVGYFITDL 
Sbjct: 65  WNNRAMSTLHAIFITAASLYMVFLSDLYSDRYFGFITFRSSTISTFCLGVSVGYFITDLG 124

Query: 122 MILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDTA 181
           MI WFYP++GGMEYVIHHLLSL+AVAYAMLTGEGQLYT+MVLISETTTPGINLRWYLDTA
Sbjct: 125 MISWFYPSLGGMEYVIHHLLSLVAVAYAMLTGEGQLYTFMVLISETTTPGINLRWYLDTA 184

Query: 182 GMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSILW 241
           GMKKSKAYLING+VIFFAWLVARILLFMYMFYHVYLHLNQVLQMH FGQ LVFVVP +L 
Sbjct: 185 GMKKSKAYLINGVVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHAFGQLLVFVVPLVLS 244

Query: 242 IMNLTWFSKIFRGLKKTLAKRQ 264
           +MNL WF+KIFRGLKKTLAKRQ
Sbjct: 245 VMNLMWFAKIFRGLKKTLAKRQ 266

BLAST of CsaV3_4G032030 vs. ExPASy TrEMBL
Match: A0A6J1J4U4 (transmembrane protein 56-like OS=Cucurbita maxima OX=3661 GN=LOC111481269 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.5e-123
Identity = 232/262 (88.55%), Postives = 250/262 (95.42%), Query Frame = 0

Query: 2   SLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRVE 61
           S +SQA +LLKYLVLSDPVVPYISIPAG+LASKLVYDLTD+IS VYFKSYSNLSRIQR+E
Sbjct: 5   SYQSQAEQLLKYLVLSDPVVPYISIPAGILASKLVYDLTDLISGVYFKSYSNLSRIQRME 64

Query: 62  WNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFITDLV 121
           WNNRAMST+HAIFITAA+LY+VFLSDLYSD+YFG ITFR+STISTF LGVSVGYFITDL 
Sbjct: 65  WNNRAMSTLHAIFITAASLYVVFLSDLYSDRYFGFITFRSSTISTFCLGVSVGYFITDLG 124

Query: 122 MILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDTA 181
           MI WFYP++GGMEYVIHHLLSL+AVAYAMLTGEGQLYT+MVLISETTTPGINLRWYLDTA
Sbjct: 125 MISWFYPSLGGMEYVIHHLLSLVAVAYAMLTGEGQLYTFMVLISETTTPGINLRWYLDTA 184

Query: 182 GMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSILW 241
           GMKKSKAYLING+VIFFAWLVARILLFMYMFYHVYLHLNQVLQMH FGQ LVFVVP +L 
Sbjct: 185 GMKKSKAYLINGVVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHAFGQLLVFVVPLVLS 244

Query: 242 IMNLTWFSKIFRGLKKTLAKRQ 264
           +MNL WF+KIFRGLKKTLAKRQ
Sbjct: 245 VMNLMWFAKIFRGLKKTLAKRQ 266

BLAST of CsaV3_4G032030 vs. ExPASy TrEMBL
Match: A0A6J1CIF9 (transmembrane protein 56-like OS=Momordica charantia OX=3673 GN=LOC111011776 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.9e-123
Identity = 233/266 (87.59%), Postives = 253/266 (95.11%), Query Frame = 0

Query: 1   MSLKS---QAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRI 60
           MSLKS   QA +LLKYLVLSDP+VPYISIPAG+LASKLV+DLTD IS+VYFKSYSNLSRI
Sbjct: 1   MSLKSYQNQAEQLLKYLVLSDPIVPYISIPAGILASKLVHDLTDSISAVYFKSYSNLSRI 60

Query: 61  QRVEWNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGPITFRNSTISTFGLGVSVGYFI 120
           QRVEWNNRAMST+HAIFITAAALY+VFLSDLYSDQ+FG ITFR+STISTF LGVSVGYFI
Sbjct: 61  QRVEWNNRAMSTLHAIFITAAALYLVFLSDLYSDQHFGLITFRSSTISTFALGVSVGYFI 120

Query: 121 TDLVMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWY 180
           TDLVMI WFYPA+GGMEYVIHHLLSL+AVAYAMLTGEGQLYTYMVLISETTTPGINLRWY
Sbjct: 121 TDLVMIFWFYPALGGMEYVIHHLLSLIAVAYAMLTGEGQLYTYMVLISETTTPGINLRWY 180

Query: 181 LDTAGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVP 240
           LDTAGMKKSKAYLING+VIFFAWLVARILLF+Y+FYHVYLHL+QVL+MH FGQFLVFVVP
Sbjct: 181 LDTAGMKKSKAYLINGVVIFFAWLVARILLFVYLFYHVYLHLDQVLKMHAFGQFLVFVVP 240

Query: 241 SILWIMNLTWFSKIFRGLKKTLAKRQ 264
            +L +MNL WF+KI +GLKKTLAKRQ
Sbjct: 241 LVLSVMNLMWFAKIVKGLKKTLAKRQ 266

BLAST of CsaV3_4G032030 vs. TAIR 10
Match: AT4G19645.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 332.0 bits (850), Expect = 4.3e-91
Identity = 168/264 (63.64%), Postives = 209/264 (79.17%), Query Frame = 0

Query: 2   SLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRVE 61
           S ++QA   +++ +L+DP +PY S+  G+   KLVYDLT++ SS++ KSYS L++I+R+E
Sbjct: 5   SYQNQAEYFVEHYLLADPFLPYTSVLVGICLCKLVYDLTELFSSIHVKSYSALTKIKRIE 64

Query: 62  WNNRAMSTVHAIFITAAALYMVFLSDLYSDQ-YFGPIT-FRNSTISTFGLGVSVGYFITD 121
           WNNR +STVHAIFI+  ALY  F SDL+SDQ     +T FRNS +STFGLGVSVGYF+ D
Sbjct: 65  WNNRGISTVHAIFISFMALYFAFFSDLFSDQRSLETLTVFRNSPLSTFGLGVSVGYFLAD 124

Query: 122 LVMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLD 181
           L MI W YP++GG EY++HH LS  AVAY++ +GE QLYTYMVLISE TTP INLRWYLD
Sbjct: 125 LGMIFWLYPSLGGSEYILHHCLSGTAVAYSLFSGEAQLYTYMVLISEVTTPEINLRWYLD 184

Query: 182 TAGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSI 241
            AG+K+SKAYL+NG+ IFFAWL ARILLF+YMFYHVY H +QV++MH FG  LVFVVP  
Sbjct: 185 IAGLKRSKAYLVNGVAIFFAWLTARILLFIYMFYHVYTHYDQVIEMHTFGYLLVFVVPIS 244

Query: 242 LWIMNLTWFSKIFRGLKKTLAKRQ 264
           L +MNL WF KI +GLKKTL KRQ
Sbjct: 245 LSVMNLIWFGKIVKGLKKTLEKRQ 268

BLAST of CsaV3_4G032030 vs. TAIR 10
Match: AT4G19645.2 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 332.0 bits (850), Expect = 4.3e-91
Identity = 168/264 (63.64%), Postives = 209/264 (79.17%), Query Frame = 0

Query: 2   SLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRVE 61
           S ++QA   +++ +L+DP +PY S+  G+   KLVYDLT++ SS++ KSYS L++I+R+E
Sbjct: 5   SYQNQAEYFVEHYLLADPFLPYTSVLVGICLCKLVYDLTELFSSIHVKSYSALTKIKRIE 64

Query: 62  WNNRAMSTVHAIFITAAALYMVFLSDLYSDQ-YFGPIT-FRNSTISTFGLGVSVGYFITD 121
           WNNR +STVHAIFI+  ALY  F SDL+SDQ     +T FRNS +STFGLGVSVGYF+ D
Sbjct: 65  WNNRGISTVHAIFISFMALYFAFFSDLFSDQRSLETLTVFRNSPLSTFGLGVSVGYFLAD 124

Query: 122 LVMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLD 181
           L MI W YP++GG EY++HH LS  AVAY++ +GE QLYTYMVLISE TTP INLRWYLD
Sbjct: 125 LGMIFWLYPSLGGSEYILHHCLSGTAVAYSLFSGEAQLYTYMVLISEVTTPEINLRWYLD 184

Query: 182 TAGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSI 241
            AG+K+SKAYL+NG+ IFFAWL ARILLF+YMFYHVY H +QV++MH FG  LVFVVP  
Sbjct: 185 IAGLKRSKAYLVNGVAIFFAWLTARILLFIYMFYHVYTHYDQVIEMHTFGYLLVFVVPIS 244

Query: 242 LWIMNLTWFSKIFRGLKKTLAKRQ 264
           L +MNL WF KI +GLKKTL KRQ
Sbjct: 245 LSVMNLIWFGKIVKGLKKTLEKRQ 268

BLAST of CsaV3_4G032030 vs. TAIR 10
Match: AT1G31300.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 328.6 bits (841), Expect = 4.7e-90
Identity = 164/262 (62.60%), Postives = 209/262 (79.77%), Query Frame = 0

Query: 2   SLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRVE 61
           S   QA  L+K  +L+DP +PY S+  G+   K+VYDL   IS+ + K+Y  L++IQR+E
Sbjct: 14  SYHHQAQHLVKNYLLADPFIPYTSVLTGIFLCKVVYDLCHFISNSHSKTYIILTKIQRIE 73

Query: 62  WNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGP-ITFRNSTISTFGLGVSVGYFITDL 121
           WNNR +STVHAIFI+A +LY VF SDL+SD++    + FR+S +S+ GLG+S+GYF+ DL
Sbjct: 74  WNNRGISTVHAIFISAMSLYFVFWSDLFSDRWHNDLVVFRSSRLSSLGLGISIGYFLADL 133

Query: 122 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 181
            MI W YP++GG+EY++HH LS +AVAY++ +GEGQLYTYMVLISE TTP INLRWYLDT
Sbjct: 134 GMIFWKYPSLGGIEYIVHHSLSGVAVAYSLFSGEGQLYTYMVLISEITTPEINLRWYLDT 193

Query: 182 AGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSIL 241
           AGMKKS AY++NG+ IF AWLVARILLF+YMFYHVYLH NQV++MH+FG  LVF VP+ L
Sbjct: 194 AGMKKSLAYVVNGVFIFLAWLVARILLFIYMFYHVYLHYNQVMRMHIFGYVLVFGVPAAL 253

Query: 242 WIMNLTWFSKIFRGLKKTLAKR 263
            +MNL WF KI RG+KKTLAKR
Sbjct: 254 GVMNLIWFGKIVRGVKKTLAKR 275

BLAST of CsaV3_4G032030 vs. TAIR 10
Match: AT1G31300.2 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 328.6 bits (841), Expect = 4.7e-90
Identity = 164/262 (62.60%), Postives = 209/262 (79.77%), Query Frame = 0

Query: 2   SLKSQAGELLKYLVLSDPVVPYISIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRVE 61
           S   QA  L+K  +L+DP +PY S+  G+   K+VYDL   IS+ + K+Y  L++IQR+E
Sbjct: 14  SYHHQAQHLVKNYLLADPFIPYTSVLTGIFLCKVVYDLCHFISNSHSKTYIILTKIQRIE 73

Query: 62  WNNRAMSTVHAIFITAAALYMVFLSDLYSDQYFGP-ITFRNSTISTFGLGVSVGYFITDL 121
           WNNR +STVHAIFI+A +LY VF SDL+SD++    + FR+S +S+ GLG+S+GYF+ DL
Sbjct: 74  WNNRGISTVHAIFISAMSLYFVFWSDLFSDRWHNDLVVFRSSRLSSLGLGISIGYFLADL 133

Query: 122 VMILWFYPAIGGMEYVIHHLLSLLAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDT 181
            MI W YP++GG+EY++HH LS +AVAY++ +GEGQLYTYMVLISE TTP INLRWYLDT
Sbjct: 134 GMIFWKYPSLGGIEYIVHHSLSGVAVAYSLFSGEGQLYTYMVLISEITTPEINLRWYLDT 193

Query: 182 AGMKKSKAYLINGIVIFFAWLVARILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSIL 241
           AGMKKS AY++NG+ IF AWLVARILLF+YMFYHVYLH NQV++MH+FG  LVF VP+ L
Sbjct: 194 AGMKKSLAYVVNGVFIFLAWLVARILLFIYMFYHVYLHYNQVMRMHIFGYVLVFGVPAAL 253

Query: 242 WIMNLTWFSKIFRGLKKTLAKR 263
            +MNL WF KI RG+KKTLAKR
Sbjct: 254 GVMNLIWFGKIVRGVKKTLAKR 275

BLAST of CsaV3_4G032030 vs. TAIR 10
Match: AT4G10360.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 229.9 bits (585), Expect = 2.3e-60
Identity = 113/238 (47.48%), Postives = 161/238 (67.65%), Query Frame = 0

Query: 25  SIPAGVLASKLVYDLTDMISSVYFKSYSNLSRIQRVEWNNRAMSTVHAIFITAAALYMVF 84
           SI +G L  K+VYDLT  IS + F  Y  L    R+EWNNR  ST HA+F + A++Y + 
Sbjct: 20  SICSGALMCKIVYDLTRFISPLLFSVYGKLDSKVRMEWNNRGFSTFHAVFTSVASIYFLV 79

Query: 85  LSDLYSDQYFGPITFRNST-ISTFGLGVSVGYFITDLVMILWFYPAIGGMEYVIHHLLSL 144
           +SD + +   G     ++T +S   +G+S+GYF+ DL MI W++P +GG+EYV HH LS+
Sbjct: 80  ISDQFDENVHGDSVINSTTRLSESVMGISLGYFLADLAMIFWYFPTLGGIEYVFHHFLSM 139

Query: 145 LAVAYAMLTGEGQLYTYMVLISETTTPGINLRWYLDTAGMKKSKAYLINGIVIFFAWLVA 204
            A+  ++ +G+ Q Y ++VL+SE TTP +NLRWYLD +G K SKAY +NGI +F  WLVA
Sbjct: 140 FAIILSVTSGQSQFYIFLVLLSEATTPFVNLRWYLDNSGQKGSKAYTLNGIALFLGWLVA 199

Query: 205 RILLFMYMFYHVYLHLNQVLQMHVFGQFLVFVVPSILWIMNLTWFSKIFRGLKKTLAK 262
           R+LLF++ F H+YLH +QV Q+   G + +  +P  L +MNL WF KI +GL KTL+K
Sbjct: 200 RVLLFIFFFVHMYLHFHQVKQVFPLGFYSLLTIPPALAVMNLLWFWKITKGLIKTLSK 257