CsaV3_3G041300 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_3G041300
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	Calcium-binding EF hand family protein
Location	chr3: 33700020 .. 33701402 (-)
RNA-Seq Expression	CsaV3_3G041300
Synteny	CsaV3_3G041300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAAAATTATAATAACTTAGTTGTACAAGAAATGGTTTGTAACTATATACTCACAGACAATTTGTCATATGAAATCCCTCCTAGTTGGTAACCCAACAAAGAAGAAAAAAAAAAAGAGCAGGCCAAGTAGGACACACTTGTTACTTTTCTTCTTCTCTTCAAATAATAAATTAAAAGAGAAAAAAACAAAACAAAACTTGAAAAATGTATTGAAGTTTCCTAACAAAACATCTCATATGAACGTTATTTTCAATGTTAATTGCACATCTTCTTGCACACATAATCACAGCAACAAGCTAGTAAATTTCAAATACCAACCTGATCAACTTCGTGTACACATATATATATATATATACACATAAACTCTTTCCCATCTCCAATTCACCACATTAACTTAGTCCCCAAACAAAATTCTATATCCATGTTCTTTAGATTAGAGTACTATTTAATCACAATGGAAACCCGGTTAACCGACATCAAAAGTGTGCAGCTCGACCCGTCACCGTTCGTGTTTGTCGATCGGATATCAATTCTACCAATTTTATTCAACTTTGCAGACAGAATACAAGAGCTGGTAAGATCTCTACCTTGGCCAATTCTTCAACCACACTCCAAAGAGAAAGCTTTATCACAATGTAATTGCTCTTCAAAATCTGGTGATCAAATCATCATCACTACTCAACAAAATTGCAGCTCAATGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTCTGGAGGGAAGAAAGGGATGGTGGTGGTGGTGATAAAGGAATAGGGGAAAGCGATGAAATGATAAGAGGTATGTTTGAAGAGAATGAACCAAGCTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATTGCCAGATTCGATCTCAATAATGATGGTAAAATTGATTTCCATGAGTTTGTTAAGTTTATGGAAGTTGCTTTGTCTTAGAATATTTTTGGTTCTACTGCTATAATTATTTGTAATAATGTTTTTCATTTTTGTGGGCGTGGTGGACATTTTCATTCACATTTTCATATGAAACCAATCCACCTTTTCATGATATATTATAAAGATTTTTGATGATATATTATAAAGATTTTACGTAATAGAACAAAAGAATATTGAATAATATACTAATGTGAACATAATATAAAAAGCAAGAAATTTAACTTAATCATAAAACTTTTATATGGTAGCATTAATTTCTTTTGGAAATTTAAAATATAATTTGTGAAGGTAGAAAAATGAAAAGAAAAATGAAAGATTCAAAGGGCGGG

mRNA sequence

ATGTTCTTTAGATTAGAGTACTATTTAATCACAATGGAAACCCGGTTAACCGACATCAAAAGTGTGCAGCTCGACCCGTCACCGTTCGTGTTTGTCGATCGGATATCAATTCTACCAATTTTATTCAACTTTGCAGACAGAATACAAGAGCTGGTAAGATCTCTACCTTGGCCAATTCTTCAACCACACTCCAAAGAGAAAGCTTTATCACAATGTAATTGCTCTTCAAAATCTGGTGATCAAATCATCATCACTACTCAACAAAATTGCAGCTCAATGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTCTGGAGGGAAGAAAGGGATGGTGGTGGTGGTGATAAAGGAATAGGGGAAAGCGATGAAATGATAAGAGGTATGTTTGAAGAGAATGAACCAAGCTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATTGCCAGATTCGATCTCAATAATGATGGTAAAATTGATTTCCATGAGTTTGTTAAGTTTATGGAAGTTGCTTTGTCTTAG

Coding sequence (CDS)

ATGTTCTTTAGATTAGAGTACTATTTAATCACAATGGAAACCCGGTTAACCGACATCAAAAGTGTGCAGCTCGACCCGTCACCGTTCGTGTTTGTCGATCGGATATCAATTCTACCAATTTTATTCAACTTTGCAGACAGAATACAAGAGCTGGTAAGATCTCTACCTTGGCCAATTCTTCAACCACACTCCAAAGAGAAAGCTTTATCACAATGTAATTGCTCTTCAAAATCTGGTGATCAAATCATCATCACTACTCAACAAAATTGCAGCTCAATGAGCAGAGAAGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTCTGGAGGGAAGAAAGGGATGGTGGTGGTGGTGATAAAGGAATAGGGGAAAGCGATGAAATGATAAGAGGTATGTTTGAAGAGAATGAACCAAGCTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATTGCCAGATTCGATCTCAATAATGATGGTAAAATTGATTTCCATGAGTTTGTTAAGTTTATGGAAGTTGCTTTGTCTTAG

Protein sequence

MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALS*

Homology

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BLAST of CsaV3_3G041300 vs. NCBI nr
Match: KGN59556.1 (hypothetical protein Csa_001548 [Cucumis sativus])

HSP 1 Score: 421.8 bits (1083), Expect = 3.5e-114
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL 60
           MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL
Sbjct: 1   MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL 60

Query: 61  QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDK 120
           QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDK
Sbjct: 61  QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDK 120

Query: 121 GIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD 180
           GIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD
Sbjct: 121 GIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD 180

Query: 181 CKTMIARFDLNNDGKIDFHEFVKFMEVALS 211
           CKTMIARFDLNNDGKIDFHEFVKFMEVALS
Sbjct: 181 CKTMIARFDLNNDGKIDFHEFVKFMEVALS 210

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BLAST of CsaV3_3G041300 vs. NCBI nr
Match: XP_004147503.1 (probable calcium-binding protein CML45 [Cucumis sativus])

HSP 1 Score: 401.0 bits (1029), Expect = 6.4e-108
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0


Query: 12  METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQ 71
           METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQ
Sbjct: 1   METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQ 60

Query: 72  CNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRG 131
           CNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRG
Sbjct: 61  CNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRG 120

Query: 132 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 191
           MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN
Sbjct: 121 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 180

Query: 192 NDGKIDFHEFVKFMEVALS 211
           NDGKIDFHEFVKFMEVALS
Sbjct: 181 NDGKIDFHEFVKFMEVALS 199

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BLAST of CsaV3_3G041300 vs. NCBI nr
Match: XP_008443616.1 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 319.3 bits (817), Expect = 2.5e-83
Identity = 169/201 (84.08%), Postives = 181/201 (90.05%), Query Frame = 0


Query: 12  METRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WPILQ-PHSKEKA 71
           M+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W ILQ  +SKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  LSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEM 131
           LSQ NCSS+S D+IIITTQQNC SMSREEVRFVMEKLELFWREE D    D+ IG SDE+
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNC-SMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEI 120

Query: 132 IRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 191
           IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF
Sbjct: 121 IRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 180

Query: 192 DLNNDGKIDFHEFVKFMEVAL 210
           DLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 DLNNDGKIDFHEFVKFMEVAL 200

[Close]

BLAST of CsaV3_3G041300 vs. NCBI nr
Match: XP_038904514.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 243.0 bits (619), Expect = 2.2e-60
Identity = 139/201 (69.15%), Postives = 154/201 (76.62%), Query Frame = 0


Query: 12  METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQ 71
           M+ RL D+ +VQL P+P VFVDR SILPILF FADRI +L+ S+P    Q  SKEKA  Q
Sbjct: 1   MQNRLPDV-NVQLIPTPLVFVDRTSILPILFTFADRIHDLI-SVPLSFFQSPSKEKASPQ 60

Query: 72  CNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGE--SDEMI 131
            NCSS+S DQ +IT Q+NC  MSREEVRFVM+KLELF  +      GD+ IGE    E +
Sbjct: 61  SNCSSQSDDQ-VITQQKNC-LMSREEVRFVMDKLELFCSK----NDGDEEIGECFGGEEL 120

Query: 132 RGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 191
             MFEENEPSLEELKQTFNVFDRNRDGFID  EL IVL LL+SN GIFIHDCK MIARFD
Sbjct: 121 TAMFEENEPSLEELKQTFNVFDRNRDGFIDAEELHIVLGLLQSNNGIFIHDCKRMIARFD 180

Query: 192 LNNDGKIDFHEFVKFMEVALS 211
            NNDGKIDF EFVKFMEVALS
Sbjct: 181 HNNDGKIDFTEFVKFMEVALS 193

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BLAST of CsaV3_3G041300 vs. NCBI nr
Match: XP_023528818.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 190.3 bits (482), Expect = 1.7e-44
Identity = 123/216 (56.94%), Postives = 140/216 (64.81%), Query Frame = 0


Query: 1   MFFRLEYYLITMETRLTDIKSVQLDPS------PFVFVDRISILPILFNFADRIQELVRS 60
           MFF+LE YL+TM      I ++QL PS      P VF+DR SI      FAD I +L+ S
Sbjct: 1   MFFKLE-YLLTMHNPQPTI-NLQLIPSTTTTTPPLVFIDRASI------FADTIHDLI-S 60

Query: 61  LPWPILQPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERD 120
           L +   Q   KEKA  Q   S   G +  +        MSREEV+ VMEKLEL+  EE D
Sbjct: 61  LVFSFFQSPPKEKAAPQSRASQSEGKEDWL--------MSREEVKSVMEKLELYCNEEED 120

Query: 121 GGGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNK 180
               D+  G  +  +RGMFEENEPSLEELKQTFNVFDRNRDGFID  EL  VL LL SN 
Sbjct: 121 -DEIDECFGRDE--VRGMFEENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNN 180

Query: 181 GIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALS 211
            IFIHDCK+MIARFD NND KIDF+EFVKFMEVALS
Sbjct: 181 TIFIHDCKSMIARFDHNNDQKIDFNEFVKFMEVALS 196

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BLAST of CsaV3_3G041300 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.0e-20
Identity = 55/118 (46.61%), Postives = 75/118 (63.56%), Query Frame = 0


Query: 93  MSREEVRFVMEKLELFWREERDGGGGDKGIGE--SDEMIRGMFEENEPSLEELKQTFNVF 152
           +SREE   VM  L LF+ +++        + E  S + +  +FEE E SLEE+KQ F+VF
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQ--------LQEQYSAKEVSSLFEEKEASLEEVKQAFDVF 142

Query: 153 DRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVA 209
           D N+DGFID  EL  VL++L   +G ++ +C  MI   D N DGKIDF+EFVKFME +
Sbjct: 143 DENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 192

[Close]

BLAST of CsaV3_3G041300 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 6.9e-20
Identity = 75/207 (36.23%), Postives = 104/207 (50.24%), Query Frame = 0


Query: 7   YYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKE 66
           Y  ITM++ L+  K  Q     F     I+   I F       +L  S  WP++Q     
Sbjct: 7   YSFITMKSSLSKCK--QSSSLSFPLFGLINFFLIGFFRWVSFAQLFFSRFWPLVQH---- 66

Query: 67  KALSQCNCSSKSGDQIIITT-------QQNCSSMSREEVRFVMEKLELFWREERDGGGGD 126
               QC    KS D    T+         +   + RE+V  VM+ L L   +E +G    
Sbjct: 67  ---QQCVSEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGLSTDQENEGLQKQ 126

Query: 127 KGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIH 186
                S + +  +FEE EPSLEE+KQ F+VFD NRDGFID  +L  VL++L   +G  + 
Sbjct: 127 ----YSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLE 186

Query: 187 DCKTMIARFDLNNDGKIDFHEFVKFME 207
           +C+ MI  FD + DG+IDF+ FVKFME
Sbjct: 187 NCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of CsaV3_3G041300 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.3e-12
Identity = 39/111 (35.14%), Postives = 66/111 (59.46%), Query Frame = 0


Query: 96  EEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRD 155
           +E+  ++EK+++        G G   I E  E+ + + +E +   E++K+ FNVFD+N D
Sbjct: 40  KELTQMIEKIDV-------NGDGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGD 99

Query: 156 GFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 207
           GFI   EL  VLS L   +G  + DCK MI + D++ DG++++ EF + M+
Sbjct: 100 GFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of CsaV3_3G041300 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-11
Identity = 41/98 (41.84%), Postives = 60/98 (61.22%), Query Frame = 0


Query: 122 IGESDEMIRGMFEENEPSL---------EELKQTFNVFDRNRDGFIDEHELFIVLSLLES 181
           I +S+ +   + EE E  L         E +K+ F +FD N+DGFIDE+EL  VLSLL  
Sbjct: 87  IDDSEALYECLIEEGEEYLLEKNEMMGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGY 146

Query: 182 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALS 211
           ++   + +C+ M+  +D N DGKIDF+EFVK +E + S
Sbjct: 147 DECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]

BLAST of CsaV3_3G041300 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 7.6e-11
Identity = 39/106 (36.79%), Postives = 63/106 (59.43%), Query Frame = 0


Query: 101 VMEKLELFWREERDGGGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDE 160
           ++EK++L        G G   I E   + + + EE +   E++++ FNVFD+NRDGFI  
Sbjct: 45  MIEKIDL-------NGDGYVDIEEFGGLYQTIMEERDEE-EDMREAFNVFDQNRDGFITV 104

Query: 161 HELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 207
            EL  VL+ L   +G  + DCK MI++ D++ DG ++F EF + M+
Sbjct: 105 EELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMK 142

[Close]

BLAST of CsaV3_3G041300 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.7e-114
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL 60
           MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL
Sbjct: 1   MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL 60

Query: 61  QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDK 120
           QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDK
Sbjct: 61  QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDK 120

Query: 121 GIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD 180
           GIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD
Sbjct: 121 GIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHD 180

Query: 181 CKTMIARFDLNNDGKIDFHEFVKFMEVALS 211
           CKTMIARFDLNNDGKIDFHEFVKFMEVALS
Sbjct: 181 CKTMIARFDLNNDGKIDFHEFVKFMEVALS 210

[Close]

BLAST of CsaV3_3G041300 vs. ExPASy TrEMBL
Match: A0A1S3B8I1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.2e-83
Identity = 169/201 (84.08%), Postives = 181/201 (90.05%), Query Frame = 0


Query: 12  METRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WPILQ-PHSKEKA 71
           M+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W ILQ  +SKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  LSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEM 131
           LSQ NCSS+S D+IIITTQQNC SMSREEVRFVMEKLELFWREE D    D+ IG SDE+
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNC-SMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEI 120

Query: 132 IRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 191
           IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF
Sbjct: 121 IRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 180

Query: 192 DLNNDGKIDFHEFVKFMEVAL 210
           DLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 DLNNDGKIDFHEFVKFMEVAL 200

[Close]

BLAST of CsaV3_3G041300 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 5.4e-44
Identity = 113/187 (60.43%), Postives = 124/187 (66.31%), Query Frame = 0


Query: 26  PSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQCNCSSKSGDQIIIT 85
           P P VF+DR SI      FAD I +LV SL +   Q   KEKA  Q   S   G +  + 
Sbjct: 21  PPPLVFIDRASI------FADTIHDLV-SLAFSFFQSPPKEKAAPQSRASQSEGKEDWL- 80

Query: 86  TQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGE--SDEMIRGMFEENEPSLEEL 145
                  MSREEV+ VMEKLEL+  EE     GD  I E    E +RGMFEENEPSLEEL
Sbjct: 81  -------MSREEVKSVMEKLELYCNEE-----GDDEIDECFGREEVRGMFEENEPSLEEL 140

Query: 146 KQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVK 205
           KQTFNVFDRNRDGFID HEL  VL LL SN  IFIHDCK+MIARFD NND KIDF+EFVK
Sbjct: 141 KQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFNEFVK 187

Query: 206 FMEVALS 211
           FMEV+LS
Sbjct: 201 FMEVSLS 187

[Close]

BLAST of CsaV3_3G041300 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 9.6e-41
Identity = 109/192 (56.77%), Postives = 122/192 (63.54%), Query Frame = 0


Query: 19  IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQCNCSSKS 78
           I S      P VF+DR SI      FA  I +LV SL +   Q   K+KA  Q   S   
Sbjct: 13  IPSTTTTTPPLVFIDRASI------FAGTIHDLV-SLAFSFFQSPPKDKAAPQSRASQSE 72

Query: 79  GDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRGMFEENEP 138
             +  +        MSREEV+ VMEKLEL+  EE D    D+  G  +  +RGMFEENEP
Sbjct: 73  SKEDWL--------MSREEVKSVMEKLELYCNEEED-DEIDECFGRDE--VRGMFEENEP 132

Query: 139 SLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDF 198
           SLEELKQTFNVFDRNRDGFID  EL  VL LL SN  IFIHDCK+MIARFD NND KIDF
Sbjct: 133 SLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDF 186

Query: 199 HEFVKFMEVALS 211
           +EFVKFMEVALS
Sbjct: 193 NEFVKFMEVALS 186

[Close]

BLAST of CsaV3_3G041300 vs. ExPASy TrEMBL
Match: A0A6J1KQS0 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111496787 PE=4 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 8.1e-40
Identity = 106/195 (54.36%), Postives = 126/195 (64.62%), Query Frame = 0


Query: 19  IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQCNCSSKS 78
           I +++L P+  + VDRISI  ILF+F DRI +L+ S PW +     KEK   Q N     
Sbjct: 7   IVNLKLAPT-LLLVDRISISSILFSFVDRIHDLI-SQPWSLFHSPPKEKVSPQGNSLESE 66

Query: 79  GDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGES---DEMIRGMFEE 138
                  TQ   SS+S+EEV+FVMEKLE F  +E         I ES    E I  +F E
Sbjct: 67  ------ATQHKNSSISKEEVKFVMEKLEFFCSDEE--------IEESWLGGEEIGAIFYE 126

Query: 139 NEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGK 198
           +EPSLEELKQTFNVFD+NRDG ID  EL  VL LL  N+G+FIHDC  MIARFD N DGK
Sbjct: 127 SEPSLEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGK 185

Query: 199 IDFHEFVKFMEVALS 211
           IDF+EFVKFME+ALS
Sbjct: 187 IDFNEFVKFMEIALS 185

[Close]

BLAST of CsaV3_3G041300 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.9e-21
Identity = 55/118 (46.61%), Postives = 75/118 (63.56%), Query Frame = 0


Query: 93  MSREEVRFVMEKLELFWREERDGGGGDKGIGE--SDEMIRGMFEENEPSLEELKQTFNVF 152
           +SREE   VM  L LF+ +++        + E  S + +  +FEE E SLEE+KQ F+VF
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQ--------LQEQYSAKEVSSLFEEKEASLEEVKQAFDVF 142

Query: 153 DRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVA 209
           D N+DGFID  EL  VL++L   +G ++ +C  MI   D N DGKIDF+EFVKFME +
Sbjct: 143 DENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 192

[Close]

BLAST of CsaV3_3G041300 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.9e-21
Identity = 75/207 (36.23%), Postives = 104/207 (50.24%), Query Frame = 0


Query: 7   YYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKE 66
           Y  ITM++ L+  K  Q     F     I+   I F       +L  S  WP++Q     
Sbjct: 7   YSFITMKSSLSKCK--QSSSLSFPLFGLINFFLIGFFRWVSFAQLFFSRFWPLVQH---- 66

Query: 67  KALSQCNCSSKSGDQIIITT-------QQNCSSMSREEVRFVMEKLELFWREERDGGGGD 126
               QC    KS D    T+         +   + RE+V  VM+ L L   +E +G    
Sbjct: 67  ---QQCVSEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGLSTDQENEGLQKQ 126

Query: 127 KGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIH 186
                S + +  +FEE EPSLEE+KQ F+VFD NRDGFID  +L  VL++L   +G  + 
Sbjct: 127 ----YSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLE 186

Query: 187 DCKTMIARFDLNNDGKIDFHEFVKFME 207
           +C+ MI  FD + DG+IDF+ FVKFME
Sbjct: 187 NCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of CsaV3_3G041300 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 72.8 bits (177), Expect = 3.7e-13
Identity = 39/111 (35.14%), Postives = 66/111 (59.46%), Query Frame = 0


Query: 96  EEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRD 155
           +E+  ++EK+++        G G   I E  E+ + + +E +   E++K+ FNVFD+N D
Sbjct: 40  KELTQMIEKIDV-------NGDGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGD 99

Query: 156 GFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 207
           GFI   EL  VLS L   +G  + DCK MI + D++ DG++++ EF + M+
Sbjct: 100 GFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of CsaV3_3G041300 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.4e-12
Identity = 41/98 (41.84%), Postives = 60/98 (61.22%), Query Frame = 0


Query: 122 IGESDEMIRGMFEENEPSL---------EELKQTFNVFDRNRDGFIDEHELFIVLSLLES 181
           I +S+ +   + EE E  L         E +K+ F +FD N+DGFIDE+EL  VLSLL  
Sbjct: 87  IDDSEALYECLIEEGEEYLLEKNEMMGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGY 146

Query: 182 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALS 211
           ++   + +C+ M+  +D N DGKIDF+EFVK +E + S
Sbjct: 147 DECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]

BLAST of CsaV3_3G041300 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 68.9 bits (167), Expect = 5.4e-12
Identity = 39/106 (36.79%), Postives = 63/106 (59.43%), Query Frame = 0


Query: 101 VMEKLELFWREERDGGGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDE 160
           ++EK++L        G G   I E   + + + EE +   E++++ FNVFD+NRDGFI  
Sbjct: 45  MIEKIDL-------NGDGYVDIEEFGGLYQTIMEERDEE-EDMREAFNVFDQNRDGFITV 104

Query: 161 HELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 207
            EL  VL+ L   +G  + DCK MI++ D++ DG ++F EF + M+
Sbjct: 105 EELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMK 142

The following BLAST results are available for this feature:

BLAST of CsaV3_3G041300 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN59556.1	3.5e-114	100.00	hypothetical protein Csa_001548 [Cucumis sativus]	[more]
XP_004147503.1	6.4e-108	100.00	probable calcium-binding protein CML45 [Cucumis sativus]	[more]
XP_008443616.1	2.5e-83	84.08	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
XP_038904514.1	2.2e-60	69.15	probable calcium-binding protein CML45 [Benincasa hispida]	[more]
XP_023528818.1	1.7e-44	56.94	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsaV3_3G041300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	4.0e-20	46.61	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	6.9e-20	36.23	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9LNE7	5.3e-12	35.14	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SN89	2.0e-11	41.84	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]
Q9SRR7	7.6e-11	36.79	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]

BLAST of CsaV3_3G041300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHQ5	1.7e-114	100.00	Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501...	[more]
A0A1S3B8I1	1.2e-83	84.08	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 P...	[more]
A0A6J1J5Q6	5.4e-44	60.43	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A6J1F854	9.6e-41	56.77	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1KQS0	8.1e-40	54.36	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114967...	[more]

BLAST of CsaV3_3G041300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	2.9e-21	46.61	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	4.9e-21	36.23	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	3.7e-13	35.14	EF hand calcium-binding protein family	[more]
AT3G47480.1	1.4e-12	41.84	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	5.4e-12	36.79	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 142..170 e-value: 2.4E-5 score: 33.8 coord: 180..208 e-value: 8.7E-4 score: 28.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 141..206 e-value: 1.8E-9 score: 37.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 138..173 score: 13.537066
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 176..210 score: 11.33333
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 142..205 e-value: 2.3309E-14 score: 63.3357
None	No IPR available	GENE3D	1.10.238.10		coord: 78..209 e-value: 3.3E-18 score: 68.3
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 6..206
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 6..206
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 151..163
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 189..201
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 91..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_3G041300.1	CsaV3_3G041300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding