CsaV3_3G039390 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_3G039390 vs. NCBI nr
Match: KAE8651154.1 (hypothetical protein Csa_002203 [Cucumis sativus])

HSP 1 Score: 2222.6 bits (5758), Expect = 0.0e+00
Identity = 1079/1079 (100.00%), Postives = 1079/1079 (100.00%), Query Frame = 0

Query: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60
            MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV
Sbjct: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60

Query: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120
            TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL
Sbjct: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120

Query: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180
            QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR
Sbjct: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180

Query: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240
            SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL
Sbjct: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240

Query: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300
            VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT
Sbjct: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300

Query: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360
            DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN
Sbjct: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360

Query: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420
            IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR
Sbjct: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420

Query: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480
            GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT
Sbjct: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480

Query: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540
            MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP
Sbjct: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540

Query: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600
            SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA
Sbjct: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600

Query: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660
            GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA
Sbjct: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660

Query: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK 720
            DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK
Sbjct: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK 720

Query: 721  PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS 780
            PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS
Sbjct: 721  PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS 780

Query: 781  GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840
            GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS
Sbjct: 781  GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840

Query: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900
            DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR
Sbjct: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900

Query: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV 960
            GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV
Sbjct: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV 960

Query: 961  LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN 1020
            LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN
Sbjct: 961  LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN 1020

Query: 1021 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 1080
            TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA
Sbjct: 1021 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 1079

BLAST of CsaV3_3G039390 vs. NCBI nr
Match: XP_011652135.2 (DNA topoisomerase 3-alpha isoform X1 [Cucumis sativus])

HSP 1 Score: 1838.5 bits (4761), Expect = 0.0e+00
Identity = 924/1079 (85.63%), Postives = 924/1079 (85.63%), Query Frame = 0

Query: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60
            MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV
Sbjct: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60

Query: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120
            TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPE                      
Sbjct: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPE---------------------- 120

Query: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240
                                                     DKLDIKRTLEQESRRAQWL
Sbjct: 181  -----------------------------------------DKLDIKRTLEQESRRAQWL 240

Query: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300
            VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE                    
Sbjct: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE-------------------- 300

Query: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360
                        RDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN
Sbjct: 301  ------------RDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360

Query: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420
            IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR
Sbjct: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420

Query: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480
            GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT
Sbjct: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480

Query: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540
            MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP
Sbjct: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540

Query: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600
            SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA
Sbjct: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600

Query: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660
            GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA
Sbjct: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660

Query: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK 720
            DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK
Sbjct: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK 720

Query: 721  PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS 780
            PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS
Sbjct: 721  PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS 780

Query: 781  GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840
            GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS
Sbjct: 781  GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840

Query: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900
            DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR
Sbjct: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900

Query: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV 960
            GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV
Sbjct: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV 924

Query: 961  LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN 1020
            LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN
Sbjct: 961  LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN 924

Query: 1021 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 1080
            TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA
Sbjct: 1021 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 924

BLAST of CsaV3_3G039390 vs. NCBI nr
Match: XP_008443145.1 (PREDICTED: DNA topoisomerase 3-alpha isoform X1 [Cucumis melo])

HSP 1 Score: 1783.8 bits (4619), Expect = 0.0e+00
Identity = 899/1079 (83.32%), Postives = 910/1079 (84.34%), Query Frame = 0

Query: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60
            MSGGR ITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV
Sbjct: 1    MSGGRPITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60

Query: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120
            TSVTGHLMELAFEERYRKW+SCDPVQLFHAPVHKFVPE                      
Sbjct: 61   TSVTGHLMELAFEERYRKWYSCDPVQLFHAPVHKFVPE---------------------- 120

Query: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240
                                                     DKLDIKRTLEQE+RR+QWL
Sbjct: 181  -----------------------------------------DKLDIKRTLEQEARRSQWL 240

Query: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300
            VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE                    
Sbjct: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE-------------------- 300

Query: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360
                        RDIH+AVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN
Sbjct: 301  ------------RDIHEAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360

Query: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420
            IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQ+HEPEEFWTINCSHNSDDGISTFSWTR
Sbjct: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQAHEPEEFWTINCSHNSDDGISTFSWTR 420

Query: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480
            GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT
Sbjct: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480

Query: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540
            MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPES LWRNP
Sbjct: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESGLWRNP 540

Query: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600
            SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA
Sbjct: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600

Query: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660
            GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA
Sbjct: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660

Query: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK 720
            DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPT+LG+ALVMGYDDMGYNLWK
Sbjct: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTSLGEALVMGYDDMGYNLWK 720

Query: 721  PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS 780
            P+LRAIMEHEMKAVSEGTKRKDEVLASCLQQMK CFLDARVNKQKLLEAMEVFFERSNGS
Sbjct: 721  PHLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKNCFLDARVNKQKLLEAMEVFFERSNGS 780

Query: 781  GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840
            GGDENRAVGEVVQLCGLCQ+SDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS
Sbjct: 781  GGDENRAVGEVVQLCGLCQESDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840

Query: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900
            DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR
Sbjct: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900

Query: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV 960
            GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGS SA+ RQSGESSVSCSSCG+PCV
Sbjct: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSGSARGRQSGESSVSCSSCGTPCV 922

Query: 961  LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN 1020
            LRTANT NNRGRKFYKCQSQECNFFVWEDNVDN NGGNS QRSNVRSSGSAPSN NPGQN
Sbjct: 961  LRTANTTNNRGRKFYKCQSQECNFFVWEDNVDNVNGGNSAQRSNVRSSGSAPSNPNPGQN 922

Query: 1021 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 1080
            TGRGRGR RGRG  R RD GQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA
Sbjct: 1021 TGRGRGRSRGRG--RGRDLGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 922

BLAST of CsaV3_3G039390 vs. NCBI nr
Match: KAA0043680.1 (DNA topoisomerase 3-alpha isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1717.6 bits (4447), Expect = 0.0e+00
Identity = 874/1105 (79.10%), Postives = 885/1105 (80.09%), Query Frame = 0

Query: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60
            MSGGR ITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV
Sbjct: 1    MSGGRPITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60

Query: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120
            TSVTGHLMELAFEERYRKW+SCDPVQLFHAPVHKFVPE                      
Sbjct: 61   TSVTGHLMELAFEERYRKWYSCDPVQLFHAPVHKFVPE---------------------- 120

Query: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240
                                                     DKLDIKRTLEQE+RR+QWL
Sbjct: 181  -----------------------------------------DKLDIKRTLEQEARRSQWL 240

Query: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300
            VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE                    
Sbjct: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE-------------------- 300

Query: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDAR------------------------ 360
                        RDIH+AVQNLVQPNKWFADAVDAR                        
Sbjct: 301  ------------RDIHEAVQNLVQPNKWFADAVDARQVIALRAKLPFCCNCLLLLLQLLL 360

Query: 361  --QEIDLRIGASFTRFQTLLLKDAFNIDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQS 420
              QEIDLRIGASFTRFQTLLLKDAFNIDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQ+
Sbjct: 361  YLQEIDLRIGASFTRFQTLLLKDAFNIDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQA 420

Query: 421  HEPEEFWTINCSHNSDDGISTFSWTRGNLFDYTCAVVFYEMCVQESTATVVNVRRQETLK 480
            HEPEEFWTINCSHNSDDGISTFSWTRGNLFDYTCAVVFYEMCVQESTATVVNVRRQETLK
Sbjct: 421  HEPEEFWTINCSHNSDDGISTFSWTRGNLFDYTCAVVFYEMCVQESTATVVNVRRQETLK 480

Query: 481  YPPHPLNTIELEKRASRYFRMSSEHTMKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVR 540
            YPPHPLNTIELEKRASRYFRMSSEHTMKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVR
Sbjct: 481  YPPHPLNTIELEKRASRYFRMSSEHTMKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVR 540

Query: 541  EQQEHQIWGAYAQRLLDPESRLWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYE 600
            EQQEHQIWGAYAQRLLDPES LWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYE
Sbjct: 541  EQQEHQIWGAYAQRLLDPESGLWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYE 600

Query: 601  LVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMILARNYLDVYRFESWGGSTIPTYV 660
            LVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMILARNYLDVYRFESWGGSTIPTYV
Sbjct: 601  LVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMILARNYLDVYRFESWGGSTIPTYV 660

Query: 661  SGQQFVPTTLTLDSGVTRPPPLLSEADLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDA 720
            SGQQFVPTTLTLDSGVTRPPPLLSEADLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDA
Sbjct: 661  SGQQFVPTTLTLDSGVTRPPPLLSEADLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDA 720

Query: 721  NTRFTPTNLGKALVMGYDDMGYNLWKPNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKT 780
            NTRFTPT+LG+ALVMGYDDMGYNLWKP+LRAIMEHEMKAVSEGTKRKDEVLASCLQQMK 
Sbjct: 721  NTRFTPTSLGEALVMGYDDMGYNLWKPHLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKN 780

Query: 781  CFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYM 840
            CFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQ+SDMVLKRNRDGNYM
Sbjct: 781  CFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQESDMVLKRNRDGNYM 840

Query: 841  VGCLAFPQCRNVVWLPGSIAEATVTSDRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGC 900
            VGCLAFPQCRNVVWLPGSIAEATVTSDRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGC
Sbjct: 841  VGCLAFPQCRNVVWLPGSIAEATVTSDRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGC 900

Query: 901  VGGCDDILRQLTEICGTGSRVSARGRGTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQS 960
            VGGCDDILRQLTEICGTGSRVS  GRGTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQS
Sbjct: 901  VGGCDDILRQLTEICGTGSRVSGTGRGTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQS 922

Query: 961  VGSRSAQRRQSGESSVSCSSCGSPCVLRTANTANNRGRKFYKCQSQECNFFVWEDNVDNG 1020
            VGS SA+ RQSGESSVSCSSCG+PCVLRTANT NNRGRKFYKCQSQECNFFVWEDNVDN 
Sbjct: 961  VGSGSARGRQSGESSVSCSSCGTPCVLRTANTTNNRGRKFYKCQSQECNFFVWEDNVDNV 922

Query: 1021 NGGNSVQRSNVRSSGSAPSNLNPGQNTGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGE 1080
            NGGNS QRSNVRSSGSAPSN NP                            ATFVSATGE
Sbjct: 1021 NGGNSAQRSNVRSSGSAPSNPNP----------------------------ATFVSATGE 922

BLAST of CsaV3_3G039390 vs. NCBI nr
Match: XP_038904101.1 (DNA topoisomerase 3-alpha isoform X1 [Benincasa hispida])

HSP 1 Score: 1713.7 bits (4437), Expect = 0.0e+00
Identity = 864/1079 (80.07%), Postives = 882/1079 (81.74%), Query Frame = 0

Query: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60
            MSGGR ITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV
Sbjct: 1    MSGGRPITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60

Query: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120
            TSVTGHLMEL FEERYRKWHSCDPVQLFHAPV K VPE                      
Sbjct: 61   TSVTGHLMELEFEERYRKWHSCDPVQLFHAPVRKVVPE---------------------- 120

Query: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240
                                                     DK+DIKRTLEQE+RR QWL
Sbjct: 181  -----------------------------------------DKMDIKRTLEQEARRCQWL 240

Query: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300
            +LWLDCDREGENIAFEVVEVCTAVN HLNIKRAHFSALIE                    
Sbjct: 241  ILWLDCDREGENIAFEVVEVCTAVNRHLNIKRAHFSALIE-------------------- 300

Query: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360
                        RDIH+AVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQT+LLKDAFN
Sbjct: 301  ------------RDIHEAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTMLLKDAFN 360

Query: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420
            IDSV DDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNS DGISTFSWTR
Sbjct: 361  IDSVVDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSGDGISTFSWTR 420

Query: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480
            GNLFDYTCAVVFYEMCVQE TATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT
Sbjct: 421  GNLFDYTCAVVFYEMCVQEPTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480

Query: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540
            MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIV+EQQEHQIWGAYAQRLLDP S LWRNP
Sbjct: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVQEQQEHQIWGAYAQRLLDPGSGLWRNP 540

Query: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600
            SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA
Sbjct: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600

Query: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660
            GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA
Sbjct: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660

Query: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK 720
            DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLG+ALVMGYDDMGYNLWK
Sbjct: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGEALVMGYDDMGYNLWK 720

Query: 721  PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS 780
            P+LRAIMEHEMKAVSEGTKRKDEVLA+CLQQMK+CFLDARVNKQKLLEAMEVFFERSNGS
Sbjct: 721  PHLRAIMEHEMKAVSEGTKRKDEVLATCLQQMKSCFLDARVNKQKLLEAMEVFFERSNGS 780

Query: 781  GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840
            GGDENRAVGEVVQ CGLCQDS+MVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEA VTS
Sbjct: 781  GGDENRAVGEVVQRCGLCQDSNMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEAAVTS 840

Query: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900
            D C LCTPGPVYLIQFKFRQLEIPPNYNANHLGC+GGCDDILRQLTEICGTGSRVSARGR
Sbjct: 841  DCCNLCTPGPVYLIQFKFRQLEIPPNYNANHLGCIGGCDDILRQLTEICGTGSRVSARGR 900

Query: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV 960
            GTSSS+VQRDN  QSVCIFCQQVGHSSNDCPSQ VGSRSA RRQSGE SVSCS+CG+PCV
Sbjct: 901  GTSSSSVQRDNTRQSVCIFCQQVGHSSNDCPSQVVGSRSAHRRQSGEYSVSCSNCGTPCV 912

Query: 961  LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN 1020
            LRTANT NNRGRKFYKCQSQECNFFVWEDNVDN NGGNS  RSN+R+SGSA  N NPG+N
Sbjct: 961  LRTANTTNNRGRKFYKCQSQECNFFVWEDNVDNSNGGNSASRSNIRTSGSAAPNPNPGRN 912

Query: 1021 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 1080
            TGRGRGRG  R            IAATFVSATGEPISDRRCFVCGD SHFANVCPNRGA
Sbjct: 1021 TGRGRGRGWSR------------IAATFVSATGEPISDRRCFVCGDASHFANVCPNRGA 912

BLAST of CsaV3_3G039390 vs. ExPASy Swiss-Prot
Match: Q9LVP1 (DNA topoisomerase 3-alpha OS=Arabidopsis thaliana OX=3702 GN=TOP3A PE=1 SV=1)

HSP 1 Score: 1261.1 bits (3262), Expect = 0.0e+00
Identity = 651/1083 (60.11%), Postives = 752/1083 (69.44%), Query Frame = 0

Query: 3    GGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIVTS 62
            GG  +TVLNVAEKPSVAKSVA ILSR    R R+GRSRYNKIFEF+YAI GQ C M++TS
Sbjct: 5    GGGPVTVLNVAEKPSVAKSVAGILSRGT-FRTREGRSRYNKIFEFDYAINGQPCRMLMTS 64

Query: 63   VTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSELQS 122
            V GHLMEL F +RYRKWHSCDP  L+ APV K VPE                        
Sbjct: 65   VIGHLMELEFADRYRKWHSCDPADLYQAPVMKHVPE------------------------ 124

Query: 123  AKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYRSG 182
                                                                        
Sbjct: 125  ------------------------------------------------------------ 184

Query: 183  IKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWLVL 242
                                                   DK DIK+TLE+E+R++ WLVL
Sbjct: 185  ---------------------------------------DKKDIKKTLEEEARKSDWLVL 244

Query: 243  WLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFITDY 302
            WLDCDREGENIAFEVV+VC AV H+L I+RAHFSALI+                      
Sbjct: 245  WLDCDREGENIAFEVVDVCRAVKHNLFIRRAHFSALID---------------------- 304

Query: 303  FYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFNID 362
                      RDIH+AVQNL  PN+ FA+AVDARQEIDLRIGASFTRFQT+LL+D F ID
Sbjct: 305  ----------RDIHEAVQNLRDPNQLFAEAVDARQEIDLRIGASFTRFQTMLLRDRFAID 364

Query: 363  SVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTRGN 422
            S  ++R+ V+SYGPCQFPTLGF+VERYWEIQ+HEPEEFWTINCSH S++G++TF+W RG+
Sbjct: 365  STGEERSRVISYGPCQFPTLGFIVERYWEIQAHEPEEFWTINCSHQSEEGLATFNWMRGH 424

Query: 423  LFDYTCAVVFYEMCVQESTATVVNV-RRQETLKYPPHPLNTIELEKRASRYFRMSSEHTM 482
            LFDY  AV+ YEMCV+E TATV+NV   +E  KYPP+PLNTIELEKRASRYFR+SSEHTM
Sbjct: 425  LFDYASAVILYEMCVEEPTATVMNVPHPRERFKYPPYPLNTIELEKRASRYFRLSSEHTM 484

Query: 483  KVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNPS 542
            KVAE+LYQAGFISYPRTETD FS RTDL  +V EQ  H  WG+YAQRLL+PE  LWRNP+
Sbjct: 485  KVAEELYQAGFISYPRTETDSFSSRTDLRAMVEEQTRHPAWGSYAQRLLEPEGGLWRNPA 544

Query: 543  GGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIAG 602
             GGHDDKAHPPIHPTKFS+GE  WS+DH  +YELVVRH+LACVSQPAV A TTVEIDIAG
Sbjct: 545  NGGHDDKAHPPIHPTKFSSGESNWSRDHLNVYELVVRHYLACVSQPAVAAETTVEIDIAG 604

Query: 603  ELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEAD 662
            E FS SGR ILA+NYL+VYRFESWGGS IP Y  GQQF+PTTLTLD+ VTRPPPLL EAD
Sbjct: 605  ERFSASGRAILAKNYLEVYRFESWGGSVIPVYEKGQQFIPTTLTLDAAVTRPPPLLCEAD 664

Query: 663  LLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWKP 722
            LLSCMDK GIGTDATMHDHIKKLLDR YATKDANTRF+PTNLG+ALVMGYDDMGY LWKP
Sbjct: 665  LLSCMDKAGIGTDATMHDHIKKLLDRGYATKDANTRFSPTNLGEALVMGYDDMGYELWKP 724

Query: 723  NLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGSG 782
            NLRA+MEH+M  VS G K K EVL +CLQQMK CFLDARV K KLLEAM +FFERSN + 
Sbjct: 725  NLRALMEHDMNEVSVGRKTKAEVLETCLQQMKACFLDARVKKSKLLEAMTIFFERSNNTD 784

Query: 783  GDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTSD 842
              E++  GEVV+ C LC +SDM L++NRDGN+MVGC+ +PQCRN VWLPG   EA+VT++
Sbjct: 785  ESESQTAGEVVRRCNLCNESDMALRKNRDGNFMVGCMNYPQCRNAVWLPGPTLEASVTTN 844

Query: 843  RCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR- 902
             C  C PGPVY I FKFRQ+ IPP ++ NHLGCVGGCDDIL+QL +ICGTGSR  AR   
Sbjct: 845  VCQSCGPGPVYKILFKFRQIGIPPGFDVNHLGCVGGCDDILKQLIDICGTGSRSQARRTP 904

Query: 903  GTS-SSNVQRDNPGQS-VCIFCQQVGHSSNDCPSQSVGSR----SAQRRQSGESSVSCSS 962
            GT+ S+N+Q  N  QS VCI CQQ GH+S +CPS+   SR    +A   ++ ES+VSC++
Sbjct: 905  GTAPSNNIQGSNTRQSNVCIHCQQRGHASTNCPSRVPASRNSRPTATNPRNDESTVSCNT 918

Query: 963  CGSPCVLRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSN 1022
            CGS CVLRTANT  NRGR+F+ C +Q C+FF WED+++N + GN+   SN  S GS    
Sbjct: 965  CGSQCVLRTANTEANRGRQFFSCPTQGCSFFAWEDSINN-SSGNATTGSN--SGGS---- 918

Query: 1023 LNPGQNTGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVC 1078
               G+   RGRGRG   G+     RG G    +FVSATGEP+S  RCF CGDPSHFAN C
Sbjct: 1025 ---GRRGSRGRGRGGRGGQSSGGRRGSG---TSFVSATGEPVSGIRCFSCGDPSHFANAC 918

BLAST of CsaV3_3G039390 vs. ExPASy Swiss-Prot
Match: C7J0A2 (DNA topoisomerase 3-alpha OS=Oryza sativa subsp. japonica OX=39947 GN=TOP3A PE=3 SV=1)

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 623/1090 (57.16%), Postives = 734/1090 (67.34%), Query Frame = 0

Query: 3    GGRSITVLNVAEKPSVAKSVATILSR-NQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIVT 62
            GG +I VLNVAEKPSVAKSVA ILSR + G+R R+GRSRYN++FEF+Y+I G++C+M+VT
Sbjct: 6    GGGAIRVLNVAEKPSVAKSVAEILSRPSGGMRSREGRSRYNRVFEFDYSIGGRACHMLVT 65

Query: 63   SVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSELQ 122
            SVTGHLMEL F++R+R+WHSCDP  LFHAPV K VP+                       
Sbjct: 66   SVTGHLMELEFDDRFRRWHSCDPADLFHAPVRKSVPQ----------------------- 125

Query: 123  SAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYRS 182
                                                                        
Sbjct: 126  ------------------------------------------------------------ 185

Query: 183  GIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWLV 242
                                                    DK DIKRTLE+E+R+ QWLV
Sbjct: 186  ----------------------------------------DKQDIKRTLEEEARKCQWLV 245

Query: 243  LWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFITD 302
            LWLDCDREGENIA+EV+++C   N  LNI RA FSALI+                     
Sbjct: 246  LWLDCDREGENIAYEVIDICAGANSRLNIWRARFSALID--------------------- 305

Query: 303  YFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFNI 362
                       R+IH+AVQ+L +PNK FADAVDARQEIDLRIGASFTRFQT+LLKDAF +
Sbjct: 306  -----------REIHEAVQHLDRPNKLFADAVDARQEIDLRIGASFTRFQTMLLKDAFVL 365

Query: 363  DSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTRG 422
            D   DDRNI+LSYGPCQFPTLGF+VER+WEIQ+HEPEEFWTINCSH SD+G ++F W RG
Sbjct: 366  DDTGDDRNIILSYGPCQFPTLGFIVERFWEIQAHEPEEFWTINCSHTSDEGTASFGWIRG 425

Query: 423  NLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHTM 482
            +LFDY+ AVV YEMCV+E  ATV NVR QE LKYPP+PL+TIEL+KRASRYFRMSSEHTM
Sbjct: 426  HLFDYSSAVVIYEMCVEEPMATVQNVRNQEKLKYPPYPLSTIELQKRASRYFRMSSEHTM 485

Query: 483  KVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNPS 542
            KVAE+LYQAGFISYPRTETD FS  TDLH IV EQ  H  WG YAQRLLDPE+RLWRNPS
Sbjct: 486  KVAEELYQAGFISYPRTETDNFSPNTDLHSIVHEQVAHPNWGTYAQRLLDPEARLWRNPS 545

Query: 543  GGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIAG 602
             GGHDDKAHPPIHPTKFSAGE  W+ +H +LYELVVRHFLAC SQPAVGA TTVEIDIAG
Sbjct: 546  NGGHDDKAHPPIHPTKFSAGETNWTDNHKKLYELVVRHFLACCSQPAVGAETTVEIDIAG 605

Query: 603  ELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEAD 662
            E F+ SGR++LA+NYLDVYRF+SWGG+ +PTY+ GQQFVPTTLTLDSG+TRPPPLL+EAD
Sbjct: 606  EQFNASGRVVLAKNYLDVYRFDSWGGTLLPTYIIGQQFVPTTLTLDSGMTRPPPLLAEAD 665

Query: 663  LLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWKP 722
            LL CMDK GIGTDATMHDHIKKLLDR YATKDANTRF+PTNLG+ALVMGYD+MGY LWKP
Sbjct: 666  LLGCMDKAGIGTDATMHDHIKKLLDRCYATKDANTRFSPTNLGEALVMGYDEMGYELWKP 725

Query: 723  NLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGSG 782
             LR++ME +MK+VS GTK K EVL +CLQQMK CFLDAR NK KL +AM  FF RS+   
Sbjct: 726  YLRSMMEADMKSVSIGTKSKSEVLENCLQQMKACFLDARANKVKLFDAMGTFFARSSRPV 785

Query: 783  GDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTSD 842
             +   ++ E V+ C  C +S+M LK+   G +MVGC  FPQCRNVVWLP S++ A VT  
Sbjct: 786  NETQNSI-ETVRPCAACNESEMFLKQRPTGEFMVGCRGFPQCRNVVWLPRSLSGAAVTDQ 845

Query: 843  RCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSR----VSA 902
             C  C PGPVY IQFKFR+ +IPPN++ +HLGC+GGCDDIL++L E+   GS       A
Sbjct: 846  VCPTCAPGPVYKIQFKFRRRDIPPNFDVDHLGCIGGCDDILKELMELSRFGSHSQTATPA 905

Query: 903  RGRGTSSSNVQRDNPGQSV-CIFCQQVGHSSNDCP-SQSVGSRSAQRRQSGESS--VSCS 962
            R +  ++S V++ +  Q +   F   V  ++   P     G RS   + SG +S  V C+
Sbjct: 906  RNQSQTASGVRQGSSRQDLHTSFHPAVQFTNGQTPVVNPQGFRSTHTQSSGNASGQVQCT 927

Query: 963  SCGSPCVLRTANTANNRGRKFYKCQSQECNFFVWEDNVDN----GNGGNS-VQRSNVRSS 1022
            SC  PCVLRTANT  NRGRKFYKCQ+  C FF WED+V+N    G GG     RS+ R S
Sbjct: 966  SCREPCVLRTANTEANRGRKFYKCQNLACGFFAWEDDVENSAPRGRGGRGRGGRSSSRQS 927

Query: 1023 GSAPSNLNPGQNTGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPS 1079
             ++ S    G   GRGR   RGRGR+ D           FV+ATGEP+    CF+CGDP+
Sbjct: 1026 SASASAGRRGGTQGRGR---RGRGRNAD--------GMMFVAATGEPVYG-SCFICGDPT 927

BLAST of CsaV3_3G039390 vs. ExPASy Swiss-Prot
Match: Q13472 (DNA topoisomerase 3-alpha OS=Homo sapiens OX=9606 GN=TOP3A PE=1 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 7.5e-179
Identity = 392/1067 (36.74%), Postives = 548/1067 (51.36%), Query Frame = 0

Query: 9    VLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIVTSVTGHLM 68
            VL VAEK   AK +A +LS N  +R R+G S++NKI+EF+Y + GQ+  M++TSV+GHL+
Sbjct: 36   VLCVAEKNDAAKGIADLLS-NGRMRRREGLSKFNKIYEFDYHLYGQNVTMVMTSVSGHLL 95

Query: 69   ELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSELQSAKVLLD 128
               F+ ++RKW SC+P+ LF A + K+ PE                              
Sbjct: 96   AHDFQMQFRKWQSCNPLVLFEAEIEKYCPE------------------------------ 155

Query: 129  KQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYRSGIKYLKN 188
                                                                        
Sbjct: 156  ------------------------------------------------------------ 215

Query: 189  NFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWLVLWLDCDR 248
            NF                                 +DIK+TLE+E+R+ Q LV+W DCDR
Sbjct: 216  NF---------------------------------VDIKKTLERETRQCQALVIWTDCDR 275

Query: 249  EGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFITDYFYIVNG 308
            EGENI FE++ VC AV  +L + RA FS +                              
Sbjct: 276  EGENIGFEIIHVCKAVKPNLQVLRARFSEITP---------------------------- 335

Query: 309  NLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFNIDSVTDDR 368
                  +  A +NL +P++  +DAVD RQE+DLRIGA+FTRFQTL L+  F    V  ++
Sbjct: 336  ----HAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIF--PEVLAEQ 395

Query: 369  NIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTRGNLFDYTC 428
              ++SYG CQFPTLGFVVER+  IQ+  PE F  I  +H+  DGI  F+W R  LF++T 
Sbjct: 396  --LISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTA 455

Query: 429  AVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHTMKVAEDLY 488
             +V Y++CV++  ATVV VR +   K+ P  L+T+ELEK ASR  R++++ TM++AE LY
Sbjct: 456  CLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLY 515

Query: 489  QAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNPSGGGHDDK 548
              G+ISYPRTET+ F +  +L  +V +Q     WGA+AQ +L+   R    P  G   D+
Sbjct: 516  TQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILE---RGGPTPRNGNKSDQ 575

Query: 549  AHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSG 608
            AHPPIHPTK++   +G   D  RLYE +VRHFLAC SQ A G  TTVEIDIA E F   G
Sbjct: 576  AHPPIHPTKYTNNLQG---DEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHG 635

Query: 609  RMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEADLLSCMDK 668
             MILARNYLDVY ++ W    +P Y  G  F P+T+ +  G T PP LL+EADL++ M+K
Sbjct: 636  LMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEK 695

Query: 669  EGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWKPNLRAIME 728
             GIGTDAT  +HI+ +  R Y     + RF P +LG  LV GYD MGY + KP+LRA +E
Sbjct: 696  HGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELE 755

Query: 729  HEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGSGGDEN--R 788
             ++K + +G K K  VL   +Q+ K  F++A    +KL EA+  +F         E+   
Sbjct: 756  ADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYP 815

Query: 789  AVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTSDRCTLC 848
            A+ E ++ C  C + DMVLK  ++G + + C+ FP+CR+ VWLP S+ EA+  S  C +C
Sbjct: 816  AMPEPIRKCPQC-NKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVC 875

Query: 849  TPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSA---RGRGTS 908
             P PVY ++ KF++  +PP      + C+GGCDD LR++ ++  +G    A    GR  +
Sbjct: 876  QPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQA 925

Query: 909  SSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCVLRT 968
            + ++ R +  Q       Q   S     S+++         +GES+    +CG   VL T
Sbjct: 936  NQSLNRMDNSQH-----PQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLT 925

Query: 969  ANTAN-NRGRKFYKCQSQECNFFVWEDNVDNGNGGNS--VQRSNVRSSGSAPSNLNPGQN 1028
                  NRGR+F+KC    CNFF+W D+ + G GG      R    S G  P    PG +
Sbjct: 996  VRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPP---GPGIH 925

Query: 1029 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDP 1068
             G     G G G        Q  +  T V   G P   R+   C  P
Sbjct: 1056 LGGFGNPGDGSGSGTSCLCSQPSVTRT-VQKDG-PNKGRQFHTCAKP 925

BLAST of CsaV3_3G039390 vs. ExPASy Swiss-Prot
Match: O70157 (DNA topoisomerase 3-alpha OS=Mus musculus OX=10090 GN=Top3a PE=2 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 2.7e-176
Identity = 385/1066 (36.12%), Postives = 545/1066 (51.13%), Query Frame = 0

Query: 9    VLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIVTSVTGHLM 68
            VL VAEK   AK +A +LS N  +R ++G S++NKI+EF+Y + GQ+  MI+TSV+GHL+
Sbjct: 36   VLCVAEKNDAAKGIADLLS-NGRMRRKEGLSKFNKIYEFDYHLYGQNVTMIMTSVSGHLL 95

Query: 69   ELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSELQSAKVLLD 128
               F+ ++RKW SC+P+ LF A + K+ PE                              
Sbjct: 96   AHDFQMQFRKWQSCNPLVLFEAEIEKYCPE------------------------------ 155

Query: 129  KQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYRSGIKYLKN 188
                                                                        
Sbjct: 156  ------------------------------------------------------------ 215

Query: 189  NFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWLVLWLDCDR 248
            NF                                 +DIK+TLE+E+   Q LV+W DCDR
Sbjct: 216  NF---------------------------------IDIKKTLERETHHCQALVIWTDCDR 275

Query: 249  EGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFITDYFYIVNG 308
            EGENI FE++ VC AV  +L + RA FS +                              
Sbjct: 276  EGENIGFEIIHVCKAVKPNLRVLRARFSEITP---------------------------- 335

Query: 309  NLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFNIDSVTDDR 368
                  +  A +NL +P++  +DAVD RQE+DLRIGA+FTRFQTL L+  F    V  ++
Sbjct: 336  ----HAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIF--PEVLAEQ 395

Query: 369  NIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTRGNLFDYTC 428
              ++SYG CQFPTLGFVVER+  IQ+  PE F  I  +H+  DG   F+W R  LF++T 
Sbjct: 396  --LISYGSCQFPTLGFVVERFKAIQAFVPEVFHKIKVTHDHKDGTVEFNWKRYRLFNHTA 455

Query: 429  AVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHTMKVAEDLY 488
             +V Y++C+++  ATVV VR +   K+ P  L+T+ELEK ASR  R++++ TM++AE LY
Sbjct: 456  CLVLYQLCMEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLY 515

Query: 489  QAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNPSGGGHDDK 548
              G+ISYPRTET+ F +  +L  +V +Q     WGA+AQ +L+   R    P  G   D+
Sbjct: 516  TQGYISYPRTETNIFPKDLNLVALVEQQTVDPHWGAFAQTILE---RGGPTPRNGSKSDQ 575

Query: 549  AHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSG 608
            AHPPIHPTK+++G +G   D  RLYE +VRHFLAC SQ A G  TTVEIDIA E F   G
Sbjct: 576  AHPPIHPTKYTSGLQG---DDRRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHG 635

Query: 609  RMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEADLLSCMDK 668
             +ILARNYLDVY ++ W    +P Y  G  F P+T+ +  G T PP LL+EADL++ M+K
Sbjct: 636  LIILARNYLDVYPYDHWSDKLLPVYEQGSHFQPSTVEMVDGETSPPQLLTEADLIALMEK 695

Query: 669  EGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWKPNLRAIME 728
             GIGTDAT  +HI+ +  R Y    ++ RF P +LG  LV GYD MGY + KP+LRA +E
Sbjct: 696  HGIGTDATHAEHIETIKARMYVGLTSDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELE 755

Query: 729  HEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFF-ERSNGSGGDE-NR 788
             ++K + EG K K +VL   +Q+ K  F++A    +KL EA+  +  ER+  +  +E   
Sbjct: 756  ADLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAKAKKLDEALSQYLGERTEMAQQEEIYP 815

Query: 789  AVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTSDRCTLC 848
            A+ E V+ C  C + DMVLK  + G + + C+ FP+CR+ VW P S+ EA+  +  C++C
Sbjct: 816  AMPEPVRKCPQC-NKDMVLKTKKSGGFYLSCMGFPECRSAVWFPDSVLEASRDNSVCSVC 875

Query: 849  TPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGRGTSSSN 908
             P PVY ++ KF++  +PP      +GC+GGCD+ L+++  +    +   A         
Sbjct: 876  QPPPVYRLKLKFKRGSLPPAMPLEFVGCIGGCDETLKEIFGLRFPRALPRASQPSGHLQA 927

Query: 909  VQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCVLRTANT 968
             Q  N   S      Q   + +  PS++V         +GES+    +CG   VL T   
Sbjct: 936  SQALNRMDSSQHNLSQPLVNRHTRPSKTVAQALLPPTTAGESNSVTCNCGREAVLLTVRK 927

Query: 969  AN-NRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQNTGRGR 1028
               N+GR FYKC + +CNFF+W D+  +  GG         +S S P   + G  +  G 
Sbjct: 996  QGPNQGRHFYKCSNGDCNFFLWADSSHSTGGGTP-------TSASGPPGSSVGCPSSVGS 927

Query: 1029 GRGRGRGRDRDRDRGQ----GQIAATFVSATGEPISDRRCFVCGDP 1068
                      D D G     GQ A T       P   R+   C  P
Sbjct: 1056 HMDGFGSLGSDSDGGTPCLCGQPAVTRTVQKDGPNKGRQFHTCAKP 927

BLAST of CsaV3_3G039390 vs. ExPASy Swiss-Prot
Match: Q8T2T7 (DNA topoisomerase 3 OS=Dictyostelium discoideum OX=44689 GN=top3 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.3e-146
Identity = 325/871 (37.31%), Postives = 438/871 (50.29%), Query Frame = 0

Query: 9   VLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRG-QSCNMIVTSVTGHL 68
           +LNVAEKPS AK +A ILS N+   VR+G S+YNK+++F Y I     C M  TSVTGHL
Sbjct: 5   ILNVAEKPSAAKEIAAILS-NKRATVREGFSKYNKLWDFKYNILNFNDCEMTFTSVTGHL 64

Query: 69  MELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSELQSAKVLL 128
           ME+   E+++ W SCDP+QLF AP+ K                                 
Sbjct: 65  MEIDVVEQFKPWASCDPIQLFDAPIRK--------------------------------- 124

Query: 129 DKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYRSGIKYLK 188
                                                                       
Sbjct: 125 ------------------------------------------------------------ 184

Query: 189 NNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWLVLWLDCD 248
                                          VP+DK  +K+TLE+E ++A  L+LWLDCD
Sbjct: 185 ------------------------------TVPSDKEPLKKTLEREIKKADILILWLDCD 244

Query: 249 REGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFITDYFYIVN 308
           REGENIAFEV+EVC          RAHFSA+I                            
Sbjct: 245 REGENIAFEVLEVCKNAKKKFEFYRAHFSAIIP--------------------------- 304

Query: 309 GNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFNIDSVTDD 368
                R+I  A +NL +PN+  + AVD R EIDLRIGA+FTRFQTL LK  F I S +D+
Sbjct: 305 -----REIDRACKNLAKPNEKDSIAVDTRMEIDLRIGAAFTRFQTLYLK-KFKIISNSDN 364

Query: 369 R---------NI------------VLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCS 428
           +         NI            ++SYGPCQFPTLGFVVERY+ I + +PE+FW ++  
Sbjct: 365 QPKTTTPANGNILNNSNNNSSGKEIISYGPCQFPTLGFVVERYFRIVNFKPEDFWHLSVV 424

Query: 429 HNSDDGIS------TFSWTRGNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPL 488
           H   D  S      TFSW R  LFDYT A + YE C+  + ATVV+V  +E+ +Y P PL
Sbjct: 425 HEKMDTSSGKMIPVTFSWCRNRLFDYTAAFILYEKCLDNTEATVVDVTSKES-RYRPVPL 484

Query: 489 NTIELEKRASRYFRMSSEHTMKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQ 548
            TIEL+K AS+  R+SS  TM+ AE+LY  G ISYPRTETD F   TDL G++  Q  + 
Sbjct: 485 TTIELQKAASKKLRISSVQTMQYAEELYTKGLISYPRTETDSFQAGTDLKGLIGNQASNP 544

Query: 549 IWGAYAQRLLDPESRLWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHF 608
            WGAYA RL++    ++  P  G ++D +HPPIHPT  + G  G   +  ++Y+ + R F
Sbjct: 545 EWGAYASRLINNNQFVY--PKSGKNNDNSHPPIHPTSSATGLSG---NLKKIYDFITRRF 604

Query: 609 LACVSQPAVGAVTTVEIDIAGELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFV 668
           LAC S+ +V A TTV IDI GE FS +G M+L   YL+VY F+      IPTY  G++F 
Sbjct: 605 LACCSEESVFANTTVTIDIQGERFSETGTMVLKLGYLEVYPFDKRNDKLIPTYQKGERFT 664

Query: 669 PTTLTLDSGVTRPPPLLSEADLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTP 728
           P  + L  G T  P  ++EA+LL+ MD   IGTDATM  HI+ + DRFY  K+ + +F P
Sbjct: 665 PKRIDLTKGTTVAPHYITEAELLTAMDNNKIGTDATMATHIQTIQDRFYVKKNESNQFVP 710

Query: 729 TNLGKALVMGYDDMGYNLWKPNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDAR 788
           +NLG +LV  Y+ MG+   KPNLRA +E ++  +S G K K EVL S +++ K  +  A 
Sbjct: 725 SNLGVSLVASYELMGFEFSKPNLRAAIEADVDKISRGQKTKQEVLLSTIEKYKQLYQLAN 710

Query: 789 VNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAF 847
            N      +   ++E ++  GG E R +      CG C +  M  K +++       L  
Sbjct: 785 QNINCFDRSFREYYEPADPKGG-EFRVLVSQFSRCGKC-NGKMQYKSDQNPEAPKRILFC 710

BLAST of CsaV3_3G039390 vs. ExPASy TrEMBL
Match: A0A0A0LBG7 (DNA topoisomerase OS=Cucumis sativus OX=3659 GN=Csa_3G813290 PE=3 SV=1)

HSP 1 Score: 1835.5 bits (4753), Expect = 0.0e+00
Identity = 922/1079 (85.45%), Postives = 923/1079 (85.54%), Query Frame = 0

Query: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60
            MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV
Sbjct: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60

Query: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120
            TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPE                      
Sbjct: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPE---------------------- 120

Query: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240
                                                     DKLDIKRTLEQESRRAQWL
Sbjct: 181  -----------------------------------------DKLDIKRTLEQESRRAQWL 240

Query: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300
            VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE                    
Sbjct: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE-------------------- 300

Query: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360
                        RDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN
Sbjct: 301  ------------RDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360

Query: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420
            IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR
Sbjct: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420

Query: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480
            GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT
Sbjct: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480

Query: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540
            MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP
Sbjct: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540

Query: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600
            SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA
Sbjct: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600

Query: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660
            GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA
Sbjct: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660

Query: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK 720
            DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLG+ALVMGYDDMGYNLWK
Sbjct: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGEALVMGYDDMGYNLWK 720

Query: 721  PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS 780
            PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS
Sbjct: 721  PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS 780

Query: 781  GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840
            GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCL FPQCRNVVWLPGSIAEATVTS
Sbjct: 781  GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLGFPQCRNVVWLPGSIAEATVTS 840

Query: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900
            DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR
Sbjct: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900

Query: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV 960
            GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV
Sbjct: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV 924

Query: 961  LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN 1020
            LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN
Sbjct: 961  LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN 924

Query: 1021 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 1080
            TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA
Sbjct: 1021 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 924

BLAST of CsaV3_3G039390 vs. ExPASy TrEMBL
Match: A0A1S3B7B9 (DNA topoisomerase OS=Cucumis melo OX=3656 GN=LOC103486821 PE=3 SV=1)

HSP 1 Score: 1783.8 bits (4619), Expect = 0.0e+00
Identity = 899/1079 (83.32%), Postives = 910/1079 (84.34%), Query Frame = 0

Query: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60
            MSGGR ITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV
Sbjct: 1    MSGGRPITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60

Query: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120
            TSVTGHLMELAFEERYRKW+SCDPVQLFHAPVHKFVPE                      
Sbjct: 61   TSVTGHLMELAFEERYRKWYSCDPVQLFHAPVHKFVPE---------------------- 120

Query: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240
                                                     DKLDIKRTLEQE+RR+QWL
Sbjct: 181  -----------------------------------------DKLDIKRTLEQEARRSQWL 240

Query: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300
            VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE                    
Sbjct: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE-------------------- 300

Query: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360
                        RDIH+AVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN
Sbjct: 301  ------------RDIHEAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360

Query: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420
            IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQ+HEPEEFWTINCSHNSDDGISTFSWTR
Sbjct: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQAHEPEEFWTINCSHNSDDGISTFSWTR 420

Query: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480
            GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT
Sbjct: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480

Query: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540
            MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPES LWRNP
Sbjct: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESGLWRNP 540

Query: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600
            SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA
Sbjct: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600

Query: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660
            GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA
Sbjct: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660

Query: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK 720
            DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPT+LG+ALVMGYDDMGYNLWK
Sbjct: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTSLGEALVMGYDDMGYNLWK 720

Query: 721  PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS 780
            P+LRAIMEHEMKAVSEGTKRKDEVLASCLQQMK CFLDARVNKQKLLEAMEVFFERSNGS
Sbjct: 721  PHLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKNCFLDARVNKQKLLEAMEVFFERSNGS 780

Query: 781  GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840
            GGDENRAVGEVVQLCGLCQ+SDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS
Sbjct: 781  GGDENRAVGEVVQLCGLCQESDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840

Query: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900
            DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR
Sbjct: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900

Query: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV 960
            GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGS SA+ RQSGESSVSCSSCG+PCV
Sbjct: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSGSARGRQSGESSVSCSSCGTPCV 922

Query: 961  LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN 1020
            LRTANT NNRGRKFYKCQSQECNFFVWEDNVDN NGGNS QRSNVRSSGSAPSN NPGQN
Sbjct: 961  LRTANTTNNRGRKFYKCQSQECNFFVWEDNVDNVNGGNSAQRSNVRSSGSAPSNPNPGQN 922

Query: 1021 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 1080
            TGRGRGR RGRG  R RD GQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA
Sbjct: 1021 TGRGRGRSRGRG--RGRDLGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 922

BLAST of CsaV3_3G039390 vs. ExPASy TrEMBL
Match: A0A5A7TKA3 (DNA topoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G00050 PE=3 SV=1)

HSP 1 Score: 1717.6 bits (4447), Expect = 0.0e+00
Identity = 874/1105 (79.10%), Postives = 885/1105 (80.09%), Query Frame = 0

Query: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60
            MSGGR ITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV
Sbjct: 1    MSGGRPITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60

Query: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120
            TSVTGHLMELAFEERYRKW+SCDPVQLFHAPVHKFVPE                      
Sbjct: 61   TSVTGHLMELAFEERYRKWYSCDPVQLFHAPVHKFVPE---------------------- 120

Query: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240
                                                     DKLDIKRTLEQE+RR+QWL
Sbjct: 181  -----------------------------------------DKLDIKRTLEQEARRSQWL 240

Query: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300
            VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE                    
Sbjct: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE-------------------- 300

Query: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDAR------------------------ 360
                        RDIH+AVQNLVQPNKWFADAVDAR                        
Sbjct: 301  ------------RDIHEAVQNLVQPNKWFADAVDARQVIALRAKLPFCCNCLLLLLQLLL 360

Query: 361  --QEIDLRIGASFTRFQTLLLKDAFNIDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQS 420
              QEIDLRIGASFTRFQTLLLKDAFNIDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQ+
Sbjct: 361  YLQEIDLRIGASFTRFQTLLLKDAFNIDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQA 420

Query: 421  HEPEEFWTINCSHNSDDGISTFSWTRGNLFDYTCAVVFYEMCVQESTATVVNVRRQETLK 480
            HEPEEFWTINCSHNSDDGISTFSWTRGNLFDYTCAVVFYEMCVQESTATVVNVRRQETLK
Sbjct: 421  HEPEEFWTINCSHNSDDGISTFSWTRGNLFDYTCAVVFYEMCVQESTATVVNVRRQETLK 480

Query: 481  YPPHPLNTIELEKRASRYFRMSSEHTMKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVR 540
            YPPHPLNTIELEKRASRYFRMSSEHTMKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVR
Sbjct: 481  YPPHPLNTIELEKRASRYFRMSSEHTMKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVR 540

Query: 541  EQQEHQIWGAYAQRLLDPESRLWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYE 600
            EQQEHQIWGAYAQRLLDPES LWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYE
Sbjct: 541  EQQEHQIWGAYAQRLLDPESGLWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYE 600

Query: 601  LVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMILARNYLDVYRFESWGGSTIPTYV 660
            LVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMILARNYLDVYRFESWGGSTIPTYV
Sbjct: 601  LVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMILARNYLDVYRFESWGGSTIPTYV 660

Query: 661  SGQQFVPTTLTLDSGVTRPPPLLSEADLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDA 720
            SGQQFVPTTLTLDSGVTRPPPLLSEADLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDA
Sbjct: 661  SGQQFVPTTLTLDSGVTRPPPLLSEADLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDA 720

Query: 721  NTRFTPTNLGKALVMGYDDMGYNLWKPNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKT 780
            NTRFTPT+LG+ALVMGYDDMGYNLWKP+LRAIMEHEMKAVSEGTKRKDEVLASCLQQMK 
Sbjct: 721  NTRFTPTSLGEALVMGYDDMGYNLWKPHLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKN 780

Query: 781  CFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYM 840
            CFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQ+SDMVLKRNRDGNYM
Sbjct: 781  CFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQESDMVLKRNRDGNYM 840

Query: 841  VGCLAFPQCRNVVWLPGSIAEATVTSDRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGC 900
            VGCLAFPQCRNVVWLPGSIAEATVTSDRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGC
Sbjct: 841  VGCLAFPQCRNVVWLPGSIAEATVTSDRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGC 900

Query: 901  VGGCDDILRQLTEICGTGSRVSARGRGTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQS 960
            VGGCDDILRQLTEICGTGSRVS  GRGTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQS
Sbjct: 901  VGGCDDILRQLTEICGTGSRVSGTGRGTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQS 922

Query: 961  VGSRSAQRRQSGESSVSCSSCGSPCVLRTANTANNRGRKFYKCQSQECNFFVWEDNVDNG 1020
            VGS SA+ RQSGESSVSCSSCG+PCVLRTANT NNRGRKFYKCQSQECNFFVWEDNVDN 
Sbjct: 961  VGSGSARGRQSGESSVSCSSCGTPCVLRTANTTNNRGRKFYKCQSQECNFFVWEDNVDNV 922

Query: 1021 NGGNSVQRSNVRSSGSAPSNLNPGQNTGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGE 1080
            NGGNS QRSNVRSSGSAPSN NP                            ATFVSATGE
Sbjct: 1021 NGGNSAQRSNVRSSGSAPSNPNP----------------------------ATFVSATGE 922

BLAST of CsaV3_3G039390 vs. ExPASy TrEMBL
Match: A0A5D3DP88 (DNA topoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005970 PE=3 SV=1)

HSP 1 Score: 1690.2 bits (4376), Expect = 0.0e+00
Identity = 872/1108 (78.70%), Postives = 900/1108 (81.23%), Query Frame = 0

Query: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60
            MSGGR ITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV
Sbjct: 1    MSGGRPITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60

Query: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120
            TSVTGHLMELAFEERYRKW+SCDPVQLFHAPVHKFVPE   + GR      P   +TS L
Sbjct: 61   TSVTGHLMELAFEERYRKWYSCDPVQLFHAPVHKFVPEF--LEGR------PTVFSTSHL 120

Query: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180
            +                                 ++ ++ +  ++               
Sbjct: 121  R---------------------------------QKFRVRVGAFQ--------------- 180

Query: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240
              +  L +N      I   G  T           S ++   DKLDIKRTLEQE+RR+QWL
Sbjct: 181  --VAVLDSN----QRIFLFGVTT-----------SELLSAKDKLDIKRTLEQEARRSQWL 240

Query: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300
            VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE                    
Sbjct: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIE-------------------- 300

Query: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360
                        RDIH+AVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN
Sbjct: 301  ------------RDIHEAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360

Query: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420
            IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQ+HEPEEFWTINCSHNSDDGISTFSWTR
Sbjct: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQAHEPEEFWTINCSHNSDDGISTFSWTR 420

Query: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480
            GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT
Sbjct: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480

Query: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540
            MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPES LWRNP
Sbjct: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESGLWRNP 540

Query: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600
            SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA
Sbjct: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600

Query: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660
            GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA
Sbjct: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660

Query: 661  DLLSCMDK------------EGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALV 720
            DLLSCMDK            EGIGTDATMHDHIKKLLDRFYATKDANTRFTPT+LG+ALV
Sbjct: 661  DLLSCMDKVTDVFANWANSGEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTSLGEALV 720

Query: 721  MGYDDMGYNLWK-PNLRAI----------------MEHEMKAVSEGTKRKDEVLASCLQQ 780
            MGYDDMG  ++   N  ++                MEHEMKAVSEGTKRKDEVLASCLQQ
Sbjct: 721  MGYDDMGKQIFSLINCLSLQVCDSTSELFFFFFFXMEHEMKAVSEGTKRKDEVLASCLQQ 780

Query: 781  MKTCFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQDSDMVLKRNRDG 840
            MK CFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQ+SDMVLKRNRDG
Sbjct: 781  MKNCFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQESDMVLKRNRDG 840

Query: 841  NYMVGCLAFPQCRNVVWLPGSIAEATVTSDRCTLCTPGPVYLIQFKFRQLEIPPNYNANH 900
            NYMVGCLAFPQCRNVVWLPGSIAEATVTSDRCTLCTPGPVYLIQFKFRQLEIPPNYNANH
Sbjct: 841  NYMVGCLAFPQCRNVVWLPGSIAEATVTSDRCTLCTPGPVYLIQFKFRQLEIPPNYNANH 900

Query: 901  LGCVGGCDDILRQLTEICGTGSRVSARGRGTSSSNVQRDNPGQSVCIFCQQVGHSSNDCP 960
            LGCVGGCDDILRQLTEIC       ARGRGTSSSNVQRDNPGQSVCIFCQQVGHSSNDCP
Sbjct: 901  LGCVGGCDDILRQLTEIC-------ARGRGTSSSNVQRDNPGQSVCIFCQQVGHSSNDCP 960

Query: 961  SQSVGSRSAQRRQSGESSVSCSSCGSPCVLRTANTANNRGRKFYKCQSQECNFFVWEDNV 1020
            SQSVGS SA+ RQSGESSVSCSSCG+PCVLRTANT NNRGRKFYKCQSQECNFFVWEDNV
Sbjct: 961  SQSVGSGSARGRQSGESSVSCSSCGTPCVLRTANTTNNRGRKFYKCQSQECNFFVWEDNV 968

Query: 1021 DNGNGGNSVQRSNVRSSGSAPSNLNPGQNTGRGRGRGRGRGRDRDRDRGQGQIAATFVSA 1080
            DN NGGNS QRSNVRSSGSAPSN NP                            ATFVSA
Sbjct: 1021 DNVNGGNSAQRSNVRSSGSAPSNPNP----------------------------ATFVSA 968

BLAST of CsaV3_3G039390 vs. ExPASy TrEMBL
Match: A0A6J1F0T9 (DNA topoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111441350 PE=3 SV=1)

HSP 1 Score: 1677.9 bits (4344), Expect = 0.0e+00
Identity = 840/1079 (77.85%), Postives = 876/1079 (81.19%), Query Frame = 0

Query: 1    MSGGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIV 60
            M+GGR ITVLNVAEKPSVAKSVATILSRNQGLRVR+GRSRYNKIFEFNYAIRGQSCNM+V
Sbjct: 1    MAGGRPITVLNVAEKPSVAKSVATILSRNQGLRVREGRSRYNKIFEFNYAIRGQSCNMLV 60

Query: 61   TSVTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSEL 120
            TSVTGHLMEL FEERYRKWHSCDPV+LF+APV K VPE                      
Sbjct: 61   TSVTGHLMELEFEERYRKWHSCDPVELFNAPVRKLVPE---------------------- 120

Query: 121  QSAKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYR 180
                                                                        
Sbjct: 121  ------------------------------------------------------------ 180

Query: 181  SGIKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWL 240
                                                     DK+DIKRTLEQE+ R QWL
Sbjct: 181  -----------------------------------------DKMDIKRTLEQEAIRCQWL 240

Query: 241  VLWLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFIT 300
            VLWLDCDREGENIAFEVVEVCT VN HLNIKRAHFSALIE                    
Sbjct: 241  VLWLDCDREGENIAFEVVEVCTMVNRHLNIKRAHFSALIE-------------------- 300

Query: 301  DYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFN 360
                        RDIH+AVQNLVQPNK++ADAVDARQEIDLRIGASFTRFQT+LLKDAFN
Sbjct: 301  ------------RDIHEAVQNLVQPNKYYADAVDARQEIDLRIGASFTRFQTMLLKDAFN 360

Query: 361  IDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTR 420
            IDSV DDRN+VLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNS DGISTFSWTR
Sbjct: 361  IDSVGDDRNVVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSGDGISTFSWTR 420

Query: 421  GNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480
            GNLFDYTCAVV YEMCV+ESTATVVNV+RQETLKYPPHPLNTIELEKRASRYFRMSSEHT
Sbjct: 421  GNLFDYTCAVVLYEMCVEESTATVVNVKRQETLKYPPHPLNTIELEKRASRYFRMSSEHT 480

Query: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNP 540
            MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIV+EQQEHQIWGAYAQRLLDP S LWRNP
Sbjct: 481  MKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVQEQQEHQIWGAYAQRLLDPGSGLWRNP 540

Query: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600
            SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA
Sbjct: 541  SGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIA 600

Query: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEA 660
            GELFSTSGRMILARNYLDVYRFESWGGSTIPTY SGQQFVPTTLTLDSGVTRPPPLLSEA
Sbjct: 601  GELFSTSGRMILARNYLDVYRFESWGGSTIPTYASGQQFVPTTLTLDSGVTRPPPLLSEA 660

Query: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWK 720
            DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLG+ALVMGYDDMGY LWK
Sbjct: 661  DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGEALVMGYDDMGYKLWK 720

Query: 721  PNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGS 780
            P+LRA+MEHEMKAVSEG KRKDEVLA+CLQQMK+CFLDAR NKQKL EAMEVFFERSNGS
Sbjct: 721  PHLRALMEHEMKAVSEGIKRKDEVLATCLQQMKSCFLDARFNKQKLFEAMEVFFERSNGS 780

Query: 781  GGDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTS 840
            GGDEN AVGEVVQ C LCQDSDMVLKRNRDGNYMVGCL FPQCRNVVWLPGSIAEA VTS
Sbjct: 781  GGDENHAVGEVVQRCRLCQDSDMVLKRNRDGNYMVGCLGFPQCRNVVWLPGSIAEAAVTS 840

Query: 841  DRCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR 900
            +RC  CTPGPVYLIQFKFRQLEIPPNYN NHLGC+GGCDDILRQLTEICGTGSR+SARGR
Sbjct: 841  NRCNSCTPGPVYLIQFKFRQLEIPPNYNPNHLGCIGGCDDILRQLTEICGTGSRISARGR 900

Query: 901  GTSSSNVQRDNPGQSVCIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCV 960
            GTSSS+VQR N  QSVC+FCQQ+ HSSNDCPSQ VGSRSA+RRQ+GESS SCS+CG+PCV
Sbjct: 901  GTSSSSVQRGNTAQSVCVFCQQLDHSSNDCPSQVVGSRSARRRQTGESSFSCSNCGTPCV 916

Query: 961  LRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSNLNPGQN 1020
            LRTANT NNRGRKFYKCQSQECNFF WED+VD+ NGGNS  RSN+RSSGSA SN NPG+N
Sbjct: 961  LRTANTTNNRGRKFYKCQSQECNFFAWEDSVDSVNGGNSAARSNIRSSGSAASNTNPGRN 916

Query: 1021 TGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVCPNRGA 1080
            TGRGRGRG+G        +GQGQIAATFVSA GEPISDRRCFVCGDPSHFANVCPNRGA
Sbjct: 1021 TGRGRGRGQG--------QGQGQIAATFVSAIGEPISDRRCFVCGDPSHFANVCPNRGA 916

BLAST of CsaV3_3G039390 vs. TAIR 10
Match: AT5G63920.1 (topoisomerase 3alpha )

HSP 1 Score: 1261.1 bits (3262), Expect = 0.0e+00
Identity = 651/1083 (60.11%), Postives = 752/1083 (69.44%), Query Frame = 0

Query: 3    GGRSITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIVTS 62
            GG  +TVLNVAEKPSVAKSVA ILSR    R R+GRSRYNKIFEF+YAI GQ C M++TS
Sbjct: 5    GGGPVTVLNVAEKPSVAKSVAGILSRGT-FRTREGRSRYNKIFEFDYAINGQPCRMLMTS 64

Query: 63   VTGHLMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSELQS 122
            V GHLMEL F +RYRKWHSCDP  L+ APV K VPE                        
Sbjct: 65   VIGHLMELEFADRYRKWHSCDPADLYQAPVMKHVPE------------------------ 124

Query: 123  AKVLLDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYRSG 182
                                                                        
Sbjct: 125  ------------------------------------------------------------ 184

Query: 183  IKYLKNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWLVL 242
                                                   DK DIK+TLE+E+R++ WLVL
Sbjct: 185  ---------------------------------------DKKDIKKTLEEEARKSDWLVL 244

Query: 243  WLDCDREGENIAFEVVEVCTAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFITDY 302
            WLDCDREGENIAFEVV+VC AV H+L I+RAHFSALI+                      
Sbjct: 245  WLDCDREGENIAFEVVDVCRAVKHNLFIRRAHFSALID---------------------- 304

Query: 303  FYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFNID 362
                      RDIH+AVQNL  PN+ FA+AVDARQEIDLRIGASFTRFQT+LL+D F ID
Sbjct: 305  ----------RDIHEAVQNLRDPNQLFAEAVDARQEIDLRIGASFTRFQTMLLRDRFAID 364

Query: 363  SVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTRGN 422
            S  ++R+ V+SYGPCQFPTLGF+VERYWEIQ+HEPEEFWTINCSH S++G++TF+W RG+
Sbjct: 365  STGEERSRVISYGPCQFPTLGFIVERYWEIQAHEPEEFWTINCSHQSEEGLATFNWMRGH 424

Query: 423  LFDYTCAVVFYEMCVQESTATVVNV-RRQETLKYPPHPLNTIELEKRASRYFRMSSEHTM 482
            LFDY  AV+ YEMCV+E TATV+NV   +E  KYPP+PLNTIELEKRASRYFR+SSEHTM
Sbjct: 425  LFDYASAVILYEMCVEEPTATVMNVPHPRERFKYPPYPLNTIELEKRASRYFRLSSEHTM 484

Query: 483  KVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNPS 542
            KVAE+LYQAGFISYPRTETD FS RTDL  +V EQ  H  WG+YAQRLL+PE  LWRNP+
Sbjct: 485  KVAEELYQAGFISYPRTETDSFSSRTDLRAMVEEQTRHPAWGSYAQRLLEPEGGLWRNPA 544

Query: 543  GGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIAG 602
             GGHDDKAHPPIHPTKFS+GE  WS+DH  +YELVVRH+LACVSQPAV A TTVEIDIAG
Sbjct: 545  NGGHDDKAHPPIHPTKFSSGESNWSRDHLNVYELVVRHYLACVSQPAVAAETTVEIDIAG 604

Query: 603  ELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEAD 662
            E FS SGR ILA+NYL+VYRFESWGGS IP Y  GQQF+PTTLTLD+ VTRPPPLL EAD
Sbjct: 605  ERFSASGRAILAKNYLEVYRFESWGGSVIPVYEKGQQFIPTTLTLDAAVTRPPPLLCEAD 664

Query: 663  LLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWKP 722
            LLSCMDK GIGTDATMHDHIKKLLDR YATKDANTRF+PTNLG+ALVMGYDDMGY LWKP
Sbjct: 665  LLSCMDKAGIGTDATMHDHIKKLLDRGYATKDANTRFSPTNLGEALVMGYDDMGYELWKP 724

Query: 723  NLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGSG 782
            NLRA+MEH+M  VS G K K EVL +CLQQMK CFLDARV K KLLEAM +FFERSN + 
Sbjct: 725  NLRALMEHDMNEVSVGRKTKAEVLETCLQQMKACFLDARVKKSKLLEAMTIFFERSNNTD 784

Query: 783  GDENRAVGEVVQLCGLCQDSDMVLKRNRDGNYMVGCLAFPQCRNVVWLPGSIAEATVTSD 842
              E++  GEVV+ C LC +SDM L++NRDGN+MVGC+ +PQCRN VWLPG   EA+VT++
Sbjct: 785  ESESQTAGEVVRRCNLCNESDMALRKNRDGNFMVGCMNYPQCRNAVWLPGPTLEASVTTN 844

Query: 843  RCTLCTPGPVYLIQFKFRQLEIPPNYNANHLGCVGGCDDILRQLTEICGTGSRVSARGR- 902
             C  C PGPVY I FKFRQ+ IPP ++ NHLGCVGGCDDIL+QL +ICGTGSR  AR   
Sbjct: 845  VCQSCGPGPVYKILFKFRQIGIPPGFDVNHLGCVGGCDDILKQLIDICGTGSRSQARRTP 904

Query: 903  GTS-SSNVQRDNPGQS-VCIFCQQVGHSSNDCPSQSVGSR----SAQRRQSGESSVSCSS 962
            GT+ S+N+Q  N  QS VCI CQQ GH+S +CPS+   SR    +A   ++ ES+VSC++
Sbjct: 905  GTAPSNNIQGSNTRQSNVCIHCQQRGHASTNCPSRVPASRNSRPTATNPRNDESTVSCNT 918

Query: 963  CGSPCVLRTANTANNRGRKFYKCQSQECNFFVWEDNVDNGNGGNSVQRSNVRSSGSAPSN 1022
            CGS CVLRTANT  NRGR+F+ C +Q C+FF WED+++N + GN+   SN  S GS    
Sbjct: 965  CGSQCVLRTANTEANRGRQFFSCPTQGCSFFAWEDSINN-SSGNATTGSN--SGGS---- 918

Query: 1023 LNPGQNTGRGRGRGRGRGRDRDRDRGQGQIAATFVSATGEPISDRRCFVCGDPSHFANVC 1078
               G+   RGRGRG   G+     RG G    +FVSATGEP+S  RCF CGDPSHFAN C
Sbjct: 1025 ---GRRGSRGRGRGGRGGQSSGGRRGSG---TSFVSATGEPVSGIRCFSCGDPSHFANAC 918

BLAST of CsaV3_3G039390 vs. TAIR 10
Match: AT2G32000.1 (DNA topoisomerase, type IA, core )

HSP 1 Score: 330.5 bits (846), Expect = 5.1e-90
Identity = 238/795 (29.94%), Postives = 349/795 (43.90%), Query Frame = 0

Query: 7   ITVLNVAEKPSVAKSVATILSRNQGLRVRDGRSRYNKIFEFNYAIRGQSCNMIVTSVTGH 66
           + VL VAEKPS+A S+A++LS  Q +  R G +   ++ EF+   RG   +  VTSV GH
Sbjct: 5   LRVLMVAEKPSIALSIASVLSHGQ-MSTRRGST---EVHEFDGMFRGFKAHYRVTSVIGH 64

Query: 67  LMELAFEERYRKWHSCDPVQLFHAPVHKFVPELVAVSGRKTNVHLPIGVTTSELQSAKVL 126
           +  + F E+Y+ W + DP  LF AP+ K                                
Sbjct: 65  VFSVDFPEKYQNWATIDPQDLFDAPIIK-------------------------------- 124

Query: 127 LDKQLKHNLMGEGNHGINEQRPGRPSRYRRIKMYIWRYRHLHRQIGGEVYWNYRSGIKYL 186
                             E  P                                      
Sbjct: 125 -----------------KESNP-------------------------------------- 184

Query: 187 KNNFHPASPISPHGHVTKIHVMAERSLRSGIVVPADKLDIKRTLEQESRRAQWLVLWLDC 246
                                               K  I R L  E+R   ++VLWLDC
Sbjct: 185 ------------------------------------KAHICRHLSNEARGCSYMVLWLDC 244

Query: 247 DREGENIAFEVVEVC--TAVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFITDYFY 306
           DREGENI FEV+E       +    + RA FS++ E                        
Sbjct: 245 DREGENICFEVIESTGFDMKDSKRKVYRARFSSVTE------------------------ 304

Query: 307 IVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAF-NIDS 366
                   +DI  A+ NLV+PN+  A AVDARQEIDL++G +F+RFQT   +  + N+D 
Sbjct: 305 --------KDISKAMDNLVEPNRDEALAVDARQEIDLKVGVAFSRFQTSYFQGKYQNLDC 364

Query: 367 VTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTRGNL 426
                  V+SYGPCQ PTLGF V+RY  I + +PE+FW +      D       W R  L
Sbjct: 365 ------RVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKDGYELQLEWERRRL 424

Query: 427 FDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRMSSEHTMKV 486
           FD   A VF ++ V+  TA V++V  ++ +K  P  LNT+ L K AS       +  M +
Sbjct: 425 FDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVASSALGFGPQTAMHL 484

Query: 487 AEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEHQIWGAYAQRLLDPESRLWRNPSGG 546
           AE LY  GFISYPRTE+  +    D    +R Q  + +WG Y QRLL     + ++    
Sbjct: 485 AERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLLSDGFHMPKS---- 544

Query: 547 GHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRHFLACVSQPAVGAVTTVEIDIAGEL 606
           G D   HPPI P + +A E     D  RLY+ V +HFL  VS       T VE+ I GE 
Sbjct: 545 GTDAGDHPPITPMR-AATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKVELSIGGET 604

Query: 607 FSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEADLL 666
           F  +G+ +  + +  +  + +     +P+++ G++     + L  G T PP  L+E++L+
Sbjct: 605 FHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPDYLTESELI 621

Query: 667 SCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWKPNL 726
           S M+K GIGTDA++  HI  + +R Y    +  +  PT LG  L+ GY  +  +L  P++
Sbjct: 665 SLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCIDPDLCLPDI 621

Query: 727 RAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDARVNKQKLLEAMEVFFERSNGSGGD 786
           R+ +E ++  V++G      V+   +QQ +  F        + +E M+  FE       D
Sbjct: 725 RSFIEQQITLVAKGQADHSHVVQHVIQQFRRKF----SYFVQQIEHMDALFEAQFSPLAD 621

Query: 787 ENRAVGEVVQLCGLC 799
             RA+ +    CG C
Sbjct: 785 SGRALSK----CGKC 621

BLAST of CsaV3_3G039390 vs. TAIR 10
Match: AT2G32000.2 (DNA topoisomerase, type IA, core )

HSP 1 Score: 317.8 bits (813), Expect = 3.4e-86
Identity = 199/579 (34.37%), Postives = 293/579 (50.60%), Query Frame = 0

Query: 223 KLDIKRTLEQESRRAQWLVLWLDCDREGENIAFEVVEVC--TAVNHHLNIKRAHFSALIE 282
           K  I R L  E+R   ++VLWLDCDREGENI FEV+E       +    + RA FS++ E
Sbjct: 67  KAHICRHLSNEARGCSYMVLWLDCDREGENICFEVIESTGFDMKDSKRKVYRARFSSVTE 126

Query: 283 RFHILNLVSILLCGFQRFITDYFYIVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEID 342
                                           +DI  A+ NLV+PN+  A AVDARQEID
Sbjct: 127 --------------------------------KDISKAMDNLVEPNRDEALAVDARQEID 186

Query: 343 LRIGASFTRFQTLLLKDAF-NIDSVTDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEE 402
           L++G +F+RFQT   +  + N+D        V+SYGPCQ PTLGF V+RY  I + +PE+
Sbjct: 187 LKVGVAFSRFQTSYFQGKYQNLDC------RVISYGPCQTPTLGFCVQRYMHINTFKPEK 246

Query: 403 FWTINCSHNSDDGISTFSWTRGNLFDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHP 462
           FW +      D       W R  LFD   A VF ++ V+  TA V++V  ++ +K  P  
Sbjct: 247 FWALRPYIRKDGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAG 306

Query: 463 LNTIELEKRASRYFRMSSEHTMKVAEDLYQAGFISYPRTETDCFSQRTDLHGIVREQQEH 522
           LNT+ L K AS       +  M +AE LY  GFISYPRTE+  +    D    +R Q  +
Sbjct: 307 LNTVNLLKVASSALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSN 366

Query: 523 QIWGAYAQRLLDPESRLWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDHHRLYELVVRH 582
            +WG Y QRLL     + ++    G D   HPPI P + +A E     D  RLY+ V +H
Sbjct: 367 PVWGGYVQRLLSDGFHMPKS----GTDAGDHPPITPMR-AATEVMVGGDAWRLYQYVCQH 426

Query: 583 FLACVSQPAVGAVTTVEIDIAGELFSTSGRMILARNYLDVYRFESWGGSTIPTYVSGQQF 642
           FL  VS       T VE+ I GE F  +G+ +  + +  +  + +     +P+++ G++ 
Sbjct: 427 FLGTVSPNCKYIRTKVELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERI 486

Query: 643 VPTTLTLDSGVTRPPPLLSEADLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDANTRFT 702
               + L  G T PP  L+E++L+S M+K GIGTDA++  HI  + +R Y    +  +  
Sbjct: 487 EVLRVELYEGNTAPPDYLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMV 546

Query: 703 PTNLGKALVMGYDDMGYNLWKPNLRAIMEHEMKAVSEGTKRKDEVLASCLQQMKTCFLDA 762
           PT LG  L+ GY  +  +L  P++R+ +E ++  V++G      V+   +QQ +  F   
Sbjct: 547 PTALGITLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKF--- 594

Query: 763 RVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLC 799
                + +E M+  FE       D  RA+ +    CG C
Sbjct: 607 -SYFVQQIEHMDALFEAQFSPLADSGRALSK----CGKC 594

BLAST of CsaV3_3G039390 vs. TAIR 10
Match: AT4G31210.1 (DNA topoisomerase, type IA, core )

HSP 1 Score: 85.9 bits (211), Expect = 2.2e-16
Identity = 147/686 (21.43%), Postives = 244/686 (35.57%), Query Frame = 0

Query: 199  HGHVTKIHVMAERSLRSGIVVPADKLDIK-----------RTLEQESRRAQWLVLWLDCD 258
            +GH+  +      + RSG V P D   +            ++++     A+ L+L  D D
Sbjct: 502  YGHIRDL------ATRSGSVRPDDDFSMVWEVPSSAWTHIKSIKVALNGAENLILASDPD 561

Query: 259  REGENIAFEVVEVCT---AVNHHLNIKRAHFSALIERFHILNLVSILLCGFQRFITDYFY 318
            REGE IA+ ++E+     A++  + + R  F  + E                        
Sbjct: 562  REGEAIAWHIIEMLQQQGALHESMTVARVVFHEITE------------------------ 621

Query: 319  IVNGNLEFRDIHDAVQNLVQPNKWFADAVDARQEIDLRIGASFTRFQTLLLKDAFNIDSV 378
                      I  A+Q+  + +     A  AR+ +D  IG + +    LL +      S 
Sbjct: 622  --------SAIKSALQSPREIDGDLVHAYLARRALDYLIGFNIS---PLLWRKLPGCPSA 681

Query: 379  TDDRNIVLSYGPCQFPTLGFVVERYWEIQSHEPEEFWTINCSHNSDDGISTFSWTRGNL- 438
                      G  Q   L  V +R  EI   +P+E+WT+       D  +TFS    +L 
Sbjct: 682  ----------GRVQSAALALVCDRESEIDGFKPQEYWTVGIKVKGKDNSATFSAHLTSLN 741

Query: 439  --------FDYTCAVVFYEMCVQESTATVVNVRRQETLKYPPHPLNTIELEKRASRYFRM 498
                              E  ++     V   +   T K PP P  T  L++ A+     
Sbjct: 742  SKRLNQLSISSEANAQDIEQRIKSEGFLVKGTKTSTTRKNPPTPYITSTLQQDAANKLHF 801

Query: 499  SSEHTMKVAEDLYQ---------AGFISYPRTETDCFSQRT--DLHGIVREQQEHQIWGA 558
            S+ HTMK+A+ LY+         AG I+Y RT+    +     D+  +V E+        
Sbjct: 802  STAHTMKLAQKLYEGVQLSDGKSAGLITYMRTDGLHIADEAIKDIQSLVAER-------- 861

Query: 559  YAQRLLDPESRLWRNPSGGGHDDKAHPPIHPTKF----SAGERGWSQDHHRLYELVVRHF 618
            Y +       R +        +  AH  I PT      S        D  +LY L+    
Sbjct: 862  YGKNFTSDSPRKYFKKVKNAQE--AHEAIRPTDIRRLPSTIASLLDADSLKLYTLIWSRA 921

Query: 619  LACVSQPAVGAVTTVEIDIAGE--LFSTSGRMILARNYLDVY------------------ 678
            +AC  +PA  A   ++I  A E  +F +S   +    Y  VY                  
Sbjct: 922  VACQMEPASIAQIQLDIGNASESIIFRSSCSKVEFLGYQAVYEDPEAKAIKNNDNDQSSE 981

Query: 679  RFESWGGSTIPTYVSGQQFVPTTLTLDSGVTRPPPLLSEADLLSCMDKEGIGTDATMHDH 738
            R E++   T+ +   G       + L    T+ PP  SE  L+  +++ GIG  +T    
Sbjct: 982  REETF--KTLSSLKDGDLLQIGEVELKQHHTQHPPRYSEGSLVKKLEELGIGRPSTYASI 1041

Query: 739  IKKLLDRFYATKDANTRFTPTNLGKALVMGYDDMGYNLWKPNLRAIMEHEMKAVSEGTKR 798
             + L  R Y T   N    P    + +          +   +  A ME E+  VS G   
Sbjct: 1042 FRVLQHRKYVT-IKNRVLYPEFRARMVSAFLTHYFTEITDYSFTADMETELDNVSGGVTE 1101

Query: 799  KDEVLASCLQQMKT-CFLDARVNKQKLLEAMEVFFERSNGSGGDENRAVGEVVQLCGLCQ 825
               +L     +    C     V + ++ + +E  +E    S       +    + C  C 
Sbjct: 1102 WKGLLRDYWTRFSAYCKRVENVQRSQVEKMLEKKYEDVLFS------LLPYPSRTCPSCM 1117

BLAST of CsaV3_3G039390 vs. TAIR 10
Match: AT5G13920.1 (GRF zinc finger / Zinc knuckle protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.4e-07
Identity = 31/76 (40.79%), Postives = 38/76 (50.00%), Query Frame = 0

Query: 917 CIFCQQVGHSSNDCPSQSVGSRSAQRRQSGESSVSCSSCGSPCVLRTANTANNRGRKFYK 976
           C  C+Q GH  NDCP +S              ++ C   G  C ++ ANT  N GRKFYK
Sbjct: 11  CFRCRQAGHWINDCPLKSY-------TDDPPPAIQCPCGGGFCEIKVANTRENPGRKFYK 70

Query: 977 C-QSQECNFFVWEDNV 992
           C  +Q C FF W D V
Sbjct: 71  CPTAQNCCFFKWCDKV 79