CsaV3_3G032650 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_3G032650
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	phosphatidylinositol-glycan biosynthesis class F protein
Location	chr3: 28167181 .. 28175088 (+)
RNA-Seq Expression	CsaV3_3G032650
Synteny	CsaV3_3G032650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCTTTAGCATTTTTTTTTCCCTTAAAACTTATTTACCAATGTGAAATTAAAATGGAGAGACGAAATGAAAAGTAACTAAAAAAAGGGTAAAAAGAGACTAAAAGAAAAAAGCATTTCCCAAGAGAAAAATGAGTTTTGGTATTTACAAACACACCTTCAGGCACTCCATTTCCCTCTCTCCGCTCACCTTCATCAAGATTTTGCATTGGGCGTTTTCCGTTTGAGCTTTAAGAAACTACAGCAATGGAGATGGAGAAGAAAGAGATGGCTTCTACATCCTCCACTCCCATTTCAATTCCAGAGGCCTTCTTCCTCCACCTTATCTCCGCCCTAGCCCTAGCCCTAGCTTTCTGGATTGCTCATTACATCTTCTCTACCCATCTCATCTCCGATCCTTCTCTTACTCTCTTCTTGATTTTGGTACTTCTCTCAATTCCCTTCATTTTCATCCTTCTTTTCCATTCTACTCCCTGCAATTTTCTTTCATTTTTCTTCTTTCTCCCTCTCCAGGTCGTTCAATCTCCCATTGTGATTCTTCTCTACAGCCGGTATCGTACGGACCGACACCAATGCTCGGTATAATTCTTCACTCTCTTAGCTAATATATACGTTCATGAGAAAATTAAACCAAATATCTGATCATGAAAACTTGGACTGTGTTCTTTGTGAATTTGCAGTACTTCAAAGCTGTGGCACGAGGATTGCTTGGGCTCCCTGCTGGTTAGTTGGAGAAAACTCATTCCACTTCCCTGCTTTTGATTTTCATTCATTTAATTACTGTTTGATGTTGTTCATGTAATTAAGTATTTTGAGGACTGAAATTCTGAATACGAAGATCATATACCTTTAATTTTCTTAAGTACTTCACACAATTTGTATGGCTCATGTCAAATATGGTGTCGTGAGAGTTATTGGACAGGAGGAAAGATTCTAAATGTAGCTATAGACTCTTTTCTTTTTTTGCTTTTTCATATTGATGTATAGTGGTTTGGTTCTTTTTACTGAGCTTGTAATAATTGGATCTATCTGCCGTTTATTATGCAGGTGCTATTATTAATGCTTTTGGAGCAATAGTTCTAGGCGCTCCTATTGGTGCTCAGTACGTGAATTAGTGTCTCAAAAGCTATATTTTTTATCTCCCGTGGATTACAAATTTTGAATATTATGGTTTGAATGGAAAATTTAATCCAGAATTTGTCCTACAAATAAATCCTCTCTGTTATAGCTGGTATTTTGTCACCAACCATTTTATGGGAACACTACTTTTCCTTTTTATCACCAACTCTTTTATGGGAACGCTACTTTTCCTTTTTCTTTCAATCAAATTTGTGAAAAAATCATTTGGAATTTTACTTGGGAAGAAGATTAATTGAAGGTAGCGTCATTAATCGTAGCTGATTGTTTTATGATTTTAAACATTGATTCTGCTCACGTCTAAAATTTCCCGAGGTTGAATCATGATGGTCATTAATAATACATTGGATAACTTGTGTGTCTTTGTGTACATGCTCTGAGTACTTCTCCTTGGCACAGATATTTCTTGAAGACCCTTAACTGGTCACTTGTGATGTCATTGTTCAATGTAAGCAAGCAATTAAGACAATTCCTTTGATGGGTATATATCTAATTATATTGTTTTAAGTTTTTCCATCTTTCAGAAACATGTACTCTTTTCATTGGCTATTGTCTTGGATCCTTAAGATGGAAAGTGAGACCTCTAACGACGGGAATTTAAGAATATTGGATGGTTAATGAAAATGGAGCTTTTCTCATATTTAGTCATTCTTATCAGTTTTCTTTTCAATGTCATTCTCTTTGTATTAAAATTCCAGGGTAGATGTAATACTGTGTTAGAAAGCAGATTTCTTCATGACTTCAACTTCACACATTGGTATAGATGTGTTAGTCCTTTAATGGTTGTGTTTCTTGTTGGTGTTCTTATAGATTGTGCCTTCAGCATGTGTCTTCGGTTCATCGTGGATAGATTGGCAGCGCCTATTTGCTTACACAAAGTATGCCTCCAAACATATTCTATCTTCCTTCTCTCAGTCTGTTCTTTTAATGTATTCTTTTACTTGCAAGATATTTAGTCAACATTTAATAAGGTAAATACAAAATTTATAATAAAATGTTTATGTGGAGGTAGTGAACGCTTTGCAATTGCAGGCCAATTGGAACTATTGATCACATGATATGCATCCCAGCACATGGTGCCATTATTGGAGCGTGGTTTGGTGCATGGCCCATGCCACTTGATTGGGAAAGGCCATGGCAGGTACTTGTTCTTCATACAGCATTGCATGTCAAAGCTCAATGACATCCAATAATGTGAATTAGCAGTCTCTGGTTTAGACTTAAATTTACAATATTGCATGATTAAGGCATTCAAATAAAAAATGGTGAAAGTCAAGTCCGCTTATGAATGTAGCAATAGTAACCTATATGATTGTGTCTAGCTTTTGTTGGATCTATTCGTGATTTCTAGGCATCCAGAGAAAAAAGAATGAGTCAAGTTTGCTCATCTCAAACTTGTGAACTTGCACTCAGACGACCTGTCCACCAAAAGTAATAATTTGATAGACATTTTACCATGTCCCCATGGTTTTATATCGAATTCAAATAAGATATTAATATTTGGTGTCAATTTATGTCTCCCTTTCCCTAGACATCATTTCCAACTCTAGTGCTCCCAATCTTGAAGGTCTTGGATTTCTCGCATCTATGTGAACTATATTAAACACAGATTACTTTAATATGCAGGAATGGCCAATTTGCGTGACTTATGGGGCAATACTGGGCTACTCAATTGCAATGGCAGCATCCTTGGTTCTCTCACACCAGAGGGGCTTGCAGCATGTTAAAAGAGACTAACTACCCAGTATTCTTTTCTAGGTAGATTAGAAAATTTCAATCACACGATGCTCCGTTTTAGAACTTCTAACCATTCATTTTCTTTTTAACATCTATCAAGACACATGCGCTGTTTCTTTGCAGCCATGTTACAATACTGCACGAAAACCTCCTTTTTTCTCCCAATGGGGACTTCTACATGTGAATTGTCTCTCAATATAATATGGTGTGCTAAAATGTGTAGATATTTTTTTTCCTTTTACACTGATCATGAAGACCTGTGTTGCCCCTTTTTGGCCACCGAAAGGGAAATGGAATACGTTTCATTCCATTCCCTACCCCACAAAAGATGGATAAGGAGGGGGAAAAGAAAAAGATGAAATCCATTAGATGCTAGAAAGAAAAAGACATTCTTCTAGGACCCCTTATCATTTGTGAAGTGATTCGACAGAGCCCATTGCGGCTTGTCTGGATCCATCCCCCCAGCTCAAAATTGCCAAAATTCTGAACAATATTAGAACTTCCACCTGTTAAATTTCTCAGGCCTACCTACCGCACTCTCCCCTTGGCTTTGCCATCTGCAGTAAAAGTCTGAACAAGGCTACTATTTGTTTGCATCATATCCAAAGTTTGGTTCATTATAGGAGATATAATACGCTGGAGATATAATACGCTTGCCATGTAAGCTTTAAACATCAAATTTTGTTCATACAAAGTTCAAAACAAAGAAAGAGATGTTGGCTGTTAGTTGATACAGCTACTGAACTACATATATTTTTAAGCACCCTACCTACATATTTACAATAAGTTGACTTGTAAGGAGGGCACTGTTTATTCATCTACACAGTAATGGAACAGAGAAGGGTAGATTTTTCTGTTTCTTTTTTCTTTTCATTAGAGATCTGGTTTATGCTAGACTTTCTATTAACAAGATGAACCAAAACAAAGGTTATCTAGAACGATCATCAGAATCATCGAACAAGTTGAACAAAAACTGAGGTAGAGAAGCTATTCTGGGAAGATTAAGCAGCAGCTCACCAACGGAATCATTCCTCGACATTGCTATGTGCTTGCTCGATTGAAAGCCATCGTGTGCAGGTAAGCCTTCTCGACTCATAGTTTCATATTCTGATGCAGTAGTGGGGTAGGTGTTGACATCGAGGCTATTATTGGCAGCTTTGGTTTGCAAGGTCTTAGCCATACAAGAATCCTTTTGCAGAAGACAGCATAGGGAATTCACCCTGGACATGATAGTCTGCTCGTCTGAGTCGGATGGATTTTGAGAATCTCCAAAAAGATACTGTGTAATTCCCTCTAGGGCTTCTCTGCTTTGTTGGTTTTCTTCAGAAAACATAGAACCGTTGGGTGTCATCTGCTGTGATAGGCAGTGTTCAATATGATTGACAAAATCACTAACAGACATTGATGGCCGAATCCCAGGAACCATGACTTGATCCCATTTATTTAACAGTCTTGAACTTTCACATCCATCGTTTCTTAATTCCTCCGTCGCTTGAGCTTCCAACCCTAAAATGTATTAATTGACAAAATCAGAATATTAGCAAAGATAAGGTTAATTGTCAGCTTGAAGATAAAGAATGTCTAGAGGGATGGAAAGCCTGTAGGC

mRNA sequence

ATGGAGATGGAGAAGAAAGAGATGGCTTCTACATCCTCCACTCCCATTTCAATTCCAGAGGCCTTCTTCCTCCACCTTATCTCCGCCCTAGCCCTAGCCCTAGCTTTCTGGATTGCTCATTACATCTTCTCTACCCATCTCATCTCCGATCCTTCTCTTACTCTCTTCTTGATTTTGGTCGTTCAATCTCCCATTGTGATTCTTCTCTACAGCCGGTATCGTACGGACCGACACCAATGCTCGTACTTCAAAGCTGTGGCACGAGGATTGCTTGGGCTCCCTGCTGGTGCTATTATTAATGCTTTTGGAGCAATAGTTCTAGGCGCTCCTATTGGTGCTCAATATTTCTTGAAGACCCTTAACTGGTCACTTGTGATGTCATTGTTCAATATTGTGCCTTCAGCATGTGTCTTCGGTTCATCGTGGATAGATTGGCAGCGCCTATTTGCTTACACAAAGCCAATTGGAACTATTGATCACATGATATGCATCCCAGCACATGGTGCCATTATTGGAGCGTGGTTTGGTGCATGGCCCATGCCACTTGATTGGGAAAGGCCATGGCAGGAATGGCCAATTTGCGTGACTTATGGGGCAATACTGGGCTACTCAATTGCAATGGCAGCATCCTTGGTTCTCTCACACCAGAGGGGCTTGCAGCATGTTAAAAGAGACTAA

Coding sequence (CDS)

ATGGAGATGGAGAAGAAAGAGATGGCTTCTACATCCTCCACTCCCATTTCAATTCCAGAGGCCTTCTTCCTCCACCTTATCTCCGCCCTAGCCCTAGCCCTAGCTTTCTGGATTGCTCATTACATCTTCTCTACCCATCTCATCTCCGATCCTTCTCTTACTCTCTTCTTGATTTTGGTCGTTCAATCTCCCATTGTGATTCTTCTCTACAGCCGGTATCGTACGGACCGACACCAATGCTCGTACTTCAAAGCTGTGGCACGAGGATTGCTTGGGCTCCCTGCTGGTGCTATTATTAATGCTTTTGGAGCAATAGTTCTAGGCGCTCCTATTGGTGCTCAATATTTCTTGAAGACCCTTAACTGGTCACTTGTGATGTCATTGTTCAATATTGTGCCTTCAGCATGTGTCTTCGGTTCATCGTGGATAGATTGGCAGCGCCTATTTGCTTACACAAAGCCAATTGGAACTATTGATCACATGATATGCATCCCAGCACATGGTGCCATTATTGGAGCGTGGTTTGGTGCATGGCCCATGCCACTTGATTGGGAAAGGCCATGGCAGGAATGGCCAATTTGCGTGACTTATGGGGCAATACTGGGCTACTCAATTGCAATGGCAGCATCCTTGGTTCTCTCACACCAGAGGGGCTTGCAGCATGTTAAAAGAGACTAA

Protein sequence

MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILVVQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTLNWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD*

Homology

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BLAST of CsaV3_3G032650 vs. NCBI nr
Match: XP_004137648.1 (phosphatidylinositol-glycan biosynthesis class F protein isoform X2 [Cucumis sativus] >KGN58643.1 hypothetical protein Csa_001578 [Cucumis sativus])

HSP 1 Score: 458.8 bits (1179), Expect = 2.8e-125
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60
           MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV
Sbjct: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60

Query: 61  VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL 120
           VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL
Sbjct: 61  VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD 226
           PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD 225

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BLAST of CsaV3_3G032650 vs. NCBI nr
Match: XP_031738426.1 (phosphatidylinositol-glycan biosynthesis class F protein isoform X1 [Cucumis sativus])

HSP 1 Score: 443.0 bits (1138), Expect = 1.6e-120
Identity = 225/255 (88.24%), Postives = 225/255 (88.24%), Query Frame = 0


Query: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLIL- 60
           MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLIL 
Sbjct: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60

Query: 61  -----------------------------VVQSPIVILLYSRYRTDRHQCSYFKAVARGL 120
                                        VVQSPIVILLYSRYRTDRHQCSYFKAVARGL
Sbjct: 61  LLSIPFIFILLFHSTPCNFLSFFFFLPLQVVQSPIVILLYSRYRTDRHQCSYFKAVARGL 120

Query: 121 LGLPAGAIINAFGAIVLGAPIGAQYFLKTLNWSLVMSLFNIVPSACVFGSSWIDWQRLFA 180
           LGLPAGAIINAFGAIVLGAPIGAQYFLKTLNWSLVMSLFNIVPSACVFGSSWIDWQRLFA
Sbjct: 121 LGLPAGAIINAFGAIVLGAPIGAQYFLKTLNWSLVMSLFNIVPSACVFGSSWIDWQRLFA 180

Query: 181 YTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVTYGAILGYSIAMAAS 226
           YTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVTYGAILGYSIAMAAS
Sbjct: 181 YTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVTYGAILGYSIAMAAS 240

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BLAST of CsaV3_3G032650 vs. NCBI nr
Match: KAA0034945.1 (glycosylphosphatidylinositol anchor biosynthesis protein 11 [Cucumis melo var. makuwa] >TYK21300.1 glycosylphosphatidylinositol anchor biosynthesis protein 11 [Cucumis melo var. makuwa])

HSP 1 Score: 442.2 bits (1136), Expect = 2.7e-120
Identity = 217/225 (96.44%), Postives = 217/225 (96.44%), Query Frame = 0


Query: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60
           MEMEKKEMAS SSTPISIPEAFFLHLIS L LALAFWIAHYIFST LISDPS TLF ILV
Sbjct: 1   MEMEKKEMASKSSTPISIPEAFFLHLISTLGLALAFWIAHYIFSTDLISDPSRTLFFILV 60

Query: 61  VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL 120
           VQSPIVILLYSRYRTDRHQCSY KAVARGLLGLP GAIINAFGAIVLGAPIGAQYFLKTL
Sbjct: 61  VQSPIVILLYSRYRTDRHQCSYLKAVARGLLGLPTGAIINAFGAIVLGAPIGAQYFLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD 226
           PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD 225

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BLAST of CsaV3_3G032650 vs. NCBI nr
Match: XP_038904140.1 (phosphatidylinositol-glycan biosynthesis class F protein [Benincasa hispida])

HSP 1 Score: 407.1 bits (1045), Expect = 9.6e-110
Identity = 198/225 (88.00%), Postives = 206/225 (91.56%), Query Frame = 0


Query: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60
           MEMEKKEMA  +S PISI EAFFLHLI AL LALAFWIA YI+ST LISDPS TLFLIL 
Sbjct: 1   MEMEKKEMACEASIPISISEAFFLHLICALGLALAFWIARYIYSTDLISDPSRTLFLILA 60

Query: 61  VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL 120
           ++ PIVILLYSRYRTDR QCSY KAVARGLLGLPAGA+INAFGAIVLGAP+GAQYF+KTL
Sbjct: 61  IECPIVILLYSRYRTDRQQCSYLKAVARGLLGLPAGAVINAFGAIVLGAPVGAQYFVKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMSLFNIVPSACVFGSSW DWQRLFAYTKP GTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSLFNIVPSACVFGSSWRDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD 226
           PLDWERPWQEWPICV+YGAILGYSIAM ASLV SH RG QHVKRD
Sbjct: 181 PLDWERPWQEWPICVSYGAILGYSIAMVASLVFSHLRGSQHVKRD 225

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BLAST of CsaV3_3G032650 vs. NCBI nr
Match: XP_022998495.1 (phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita maxima])

HSP 1 Score: 380.2 bits (975), Expect = 1.3e-101
Identity = 185/226 (81.86%), Postives = 200/226 (88.50%), Query Frame = 0


Query: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60
           ME EKKEM S +S P+SI EAFF+HLI  L LALAF IA +++S  LISDPS TLFLIL+
Sbjct: 1   METEKKEMTSKASPPVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 61  VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL 120
           V+ PIVILLYSRYRTDR QCSY KA+ARG+LGL AGAIINAFGAIVLGAPIGAQY LKTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAMARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMS F +VPSACVFGSSW+DWQRLFAYTKP GTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFGVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQR-GLQHVKRD 226
           PLDWERPWQEWPICV+YGA++GYSIAM ASL  SH R GLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

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BLAST of CsaV3_3G032650 vs. ExPASy Swiss-Prot
Match: Q9Y7P2 (Uncharacterized protein C1450.15 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC1450.15 PE=3 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 9.0e-18
Identity = 49/130 (37.69%), Postives = 71/130 (54.62%), Query Frame = 0


Query: 84  KAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTLNWSLVMSLFNIVPSACVFGSSWI 143
           K +  G   +  G+++ +F  +  GAP+   + L T   +L +S+F + P A     +  
Sbjct: 367 KRLLAGAASMLIGSLLISFILVAFGAPLLHDFHL-TYFCALTLSVFTVYPLASTLAFNTE 426

Query: 144 DWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVTYGAILGY 203
            WQR F   K    I  M  + + G IIGAWFGA+P+PLDW+RPWQ WPI +  GA LGY
Sbjct: 427 QWQR-FLTLKSFNVIGSM-QLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFLGY 486

Query: 204 SIAMAASLVL 214
           + A     +L
Sbjct: 487 AFAAIVGEIL 493

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BLAST of CsaV3_3G032650 vs. ExPASy Swiss-Prot
Match: O09101 (Phosphatidylinositol-glycan biosynthesis class F protein OS=Mus musculus OX=10090 GN=Pigf PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.7e-16
Identity = 43/110 (39.09%), Postives = 63/110 (57.27%), Query Frame = 0


Query: 105 IVLGAPIGAQYFLKTLNWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICI 164
           ++ GAP+  +  L+T  +++V+S F  VP  C+ G +   W R+F+        ++ + I
Sbjct: 99  VLYGAPL-IELVLETFLFAVVLSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQI 158

Query: 165 PAHGAIIGAWFGAWPMPLDWERPWQEWPICVTYGAILGYSIAMAASLVLS 215
               +  GAW GA+P+PLDWERPWQ WPI  T GA  GY     A LV+S
Sbjct: 159 TTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATFGY----VAGLVIS 203

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BLAST of CsaV3_3G032650 vs. ExPASy Swiss-Prot
Match: Q07326 (Phosphatidylinositol-glycan biosynthesis class F protein OS=Homo sapiens OX=9606 GN=PIGF PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-15
Identity = 60/204 (29.41%), Postives = 95/204 (46.57%), Query Frame = 0


Query: 18  IPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLI---LVVQSPIVILLYSRYR 77
           I    + HL+   ++ L+ +I         I +  LT   I    V    +V+ L  +  
Sbjct: 6   IKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPN 65

Query: 78  TDRHQCSYFKAVARGLLGLPAGAIINAFG----AIVLGAPIGAQYFLKTLNWSLVMSLFN 137
           T   + S    V  G L      +++ F      ++ GAP+  +  L+T  +++++S F 
Sbjct: 66  TSSKRSSLSHKVT-GFLKCCIYFLMSCFSFHVIFVLYGAPL-IELALETFLFAVILSTFT 125

Query: 138 IVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQE 197
            VP  C+ G +   W R+F+        ++ + I    + +GAW GA P+PLDWERPWQ 
Sbjct: 126 TVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQV 185

Query: 198 WPICVTYGAILGYSIAMAASLVLS 215
           WPI  T GA  GY     A LV+S
Sbjct: 186 WPISCTLGATFGY----VAGLVIS 203

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BLAST of CsaV3_3G032650 vs. ExPASy Swiss-Prot
Match: Q6C741 (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=GPI11 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.6e-11
Identity = 37/118 (31.36%), Postives = 56/118 (47.46%), Query Frame = 0


Query: 102 FGAIVLGAPIGAQYFLKTLNWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHM 161
           FG +VL           T   ++ MS+  ++P    +      W  + A  +P   +DH+
Sbjct: 102 FGLLVLFGAPAISLVHNTFVCAMHMSILAVLPLFFTYHLDSKVWADIIAMRRP---LDHV 161

Query: 162 ICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGL 220
                   +IGAW GA P+P DW+RPWQ+WPI +  GA LGY +     + L   + L
Sbjct: 162 YAASV-CTLIGAWLGAIPIPYDWDRPWQQWPITILAGAYLGYFVGTLGGIALELTKSL 215

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BLAST of CsaV3_3G032650 vs. ExPASy Swiss-Prot
Match: P0C148 (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=GPI11 PE=3 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 2.1e-11
Identity = 47/133 (35.34%), Postives = 64/133 (48.12%), Query Frame = 0


Query: 90  LLGLPAGAIINAFGAIVLGAPI---GAQYFLKTLNWSLVMSLFNIVPSACVFGSSWIDWQ 149
           LL L A   I+A   ++ GAP        FL  LN    +SL  + P     G+    W+
Sbjct: 136 LLTLIATPPIHAL-MVLFGAPFLTHAPHTFLCALN----LSLLTLFPLFYTRGAEASAWR 195

Query: 150 RLFAYTKPIG-TIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVTYGAILGYSI 209
            L  +T PI  ++  ++     GA  GAW GA P+PLDW+R WQ WP+ V  G  +GY+I
Sbjct: 196 ALAGFTAPIDESVGGLV-----GACFGAWLGAVPIPLDWDRDWQRWPVTVLTGIYVGYAI 255

Query: 210 AMAASLVLSHQRG 219
                  L   RG
Sbjct: 256 GSYGGRTLLRIRG 258

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BLAST of CsaV3_3G032650 vs. ExPASy TrEMBL
Match: A0A0A0L9Y3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710220 PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.4e-125
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60
           MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV
Sbjct: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60

Query: 61  VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL 120
           VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL
Sbjct: 61  VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD 226
           PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD 225

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BLAST of CsaV3_3G032650 vs. ExPASy TrEMBL
Match: A0A5A7T0F5 (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00080 PE=3 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.3e-120
Identity = 217/225 (96.44%), Postives = 217/225 (96.44%), Query Frame = 0


Query: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60
           MEMEKKEMAS SSTPISIPEAFFLHLIS L LALAFWIAHYIFST LISDPS TLF ILV
Sbjct: 1   MEMEKKEMASKSSTPISIPEAFFLHLISTLGLALAFWIAHYIFSTDLISDPSRTLFFILV 60

Query: 61  VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL 120
           VQSPIVILLYSRYRTDRHQCSY KAVARGLLGLP GAIINAFGAIVLGAPIGAQYFLKTL
Sbjct: 61  VQSPIVILLYSRYRTDRHQCSYLKAVARGLLGLPTGAIINAFGAIVLGAPIGAQYFLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD 226
           PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQRGLQHVKRD 225

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BLAST of CsaV3_3G032650 vs. ExPASy TrEMBL
Match: A0A6J1KCP2 (phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita maxima OX=3661 GN=LOC111493110 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 6.1e-102
Identity = 185/226 (81.86%), Postives = 200/226 (88.50%), Query Frame = 0


Query: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60
           ME EKKEM S +S P+SI EAFF+HLI  L LALAF IA +++S  LISDPS TLFLIL+
Sbjct: 1   METEKKEMTSKASPPVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 61  VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL 120
           V+ PIVILLYSRYRTDR QCSY KA+ARG+LGL AGAIINAFGAIVLGAPIGAQY LKTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAMARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMS F +VPSACVFGSSW+DWQRLFAYTKP GTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFGVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQR-GLQHVKRD 226
           PLDWERPWQEWPICV+YGA++GYSIAM ASL  SH R GLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

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BLAST of CsaV3_3G032650 vs. ExPASy TrEMBL
Match: A0A6J1GAT8 (phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita moschata OX=3662 GN=LOC111452477 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 6.1e-102
Identity = 186/226 (82.30%), Postives = 201/226 (88.94%), Query Frame = 0


Query: 1   MEMEKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILV 60
           ME EKKEMAS +S  +SI EAFF+HLI  L LALAF IA +++S  LISDPS TLFLIL+
Sbjct: 1   METEKKEMASKASPSVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 61  VQSPIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTL 120
           V+ PIVILLYSRYRTDR QCSY KAVARG+LGL AGAIINAFGAIVLGAPIGAQY LKTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAVARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMS F++VPSACVFGSSW+DWQRLFAYTKP GTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFSVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVTYGAILGYSIAMAASLVLSHQR-GLQHVKRD 226
           PLDWERPWQEWPICV+YGA++GYSIAM ASL  SH R GLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

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BLAST of CsaV3_3G032650 vs. ExPASy TrEMBL
Match: A0A6J1DKK8 (phosphatidylinositol-glycan biosynthesis class F protein OS=Momordica charantia OX=3673 GN=LOC111020928 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 9.1e-98
Identity = 177/222 (79.73%), Postives = 193/222 (86.94%), Query Frame = 0


Query: 5   KKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILVVQSP 64
           +KEMAS + T +SI EAF +HLI  L LALA WIA YI+ST LIS+PS TLFLI  ++ P
Sbjct: 4   EKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECP 63

Query: 65  IVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTLNWSL 124
           IVILLYSRYR DR QCSY KAV RG+LGLPAGA+INAFGAIVLGAP+GAQYF KTLNWSL
Sbjct: 64  IVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSL 123

Query: 125 VMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDW 184
           VMSLFNIVPSACVFGSSW+DWQRLFAYTKP GTIDHMICIPAHGAIIGAWFGAWPMPLDW
Sbjct: 124 VMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW 183

Query: 185 ERPWQEWPICVTYGAILGYSIAMAASLVLSHQR-GLQHVKRD 226
           ERPWQEWPICV+YGA++GYS +M ASL  S  R GLQHVK D
Sbjct: 184 ERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 225

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BLAST of CsaV3_3G032650 vs. TAIR 10
Match: AT1G16040.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic process; LOCATED IN: integral to membrane, endoplasmic reticulum membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GPI biosynthesis protein Pig-F (InterPro:IPR009580); Has 280 Blast hits to 280 proteins in 133 species: Archae - 0; Bacteria - 0; Metazoa - 113; Fungi - 111; Plants - 44; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink). )

HSP 1 Score: 245.0 bits (624), Expect = 5.9e-65
Identity = 119/219 (54.34%), Postives = 156/219 (71.23%), Query Frame = 0


Query: 4   EKKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILVVQS 63
           EKK    + S  IS   AF +++I+ L L   F +    +S  LISDP+LTL L+ +++ 
Sbjct: 6   EKKNPEISVSITISTWGAFAVYVITGLFLIFGFHVVRNKYSVDLISDPTLTLRLLWIIEF 65

Query: 64  PIVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTLNWS 123
           PIV+++YS +R +  +CSYF+AV R L+GL AGA+INA GA+ LGAPIG Q   KT++WS
Sbjct: 66  PIVVIIYSLFRRNPEKCSYFRAVGRSLVGLIAGALINALGAVSLGAPIGMQSLSKTIHWS 125

Query: 124 LVMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLD 183
            +MS+F +VP+  V G+SWIDW R+FA  KPIG I+HM+ +PA+GAIIG WFGAWPMPLD
Sbjct: 126 FLMSVFTVVPATAVLGASWIDWHRIFASLKPIGIIEHMLLVPAYGAIIGGWFGAWPMPLD 185

Query: 184 WERPWQEWPICVTYGAILGYSIAMAASL--VLSHQRGLQ 221
           WERPWQEWPICV YGAI GY      SL  +LS  + L+
Sbjct: 186 WERPWQEWPICVCYGAIGGYIGGQMVSLLTLLSEHKNLK 224

The following BLAST results are available for this feature:

BLAST of CsaV3_3G032650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137648.1	2.8e-125	100.00	phosphatidylinositol-glycan biosynthesis class F protein isoform X2 [Cucumis sat...	[more]
XP_031738426.1	1.6e-120	88.24	phosphatidylinositol-glycan biosynthesis class F protein isoform X1 [Cucumis sat...	[more]
KAA0034945.1	2.7e-120	96.44	glycosylphosphatidylinositol anchor biosynthesis protein 11 [Cucumis melo var. m...	[more]
XP_038904140.1	9.6e-110	88.00	phosphatidylinositol-glycan biosynthesis class F protein [Benincasa hispida]	[more]
XP_022998495.1	1.3e-101	81.86	phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita maxima]	[more]

BLAST of CsaV3_3G032650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y7P2	9.0e-18	37.69	Uncharacterized protein C1450.15 OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]
O09101	1.7e-16	39.09	Phosphatidylinositol-glycan biosynthesis class F protein OS=Mus musculus OX=1009...	[more]
Q07326	1.4e-15	29.41	Phosphatidylinositol-glycan biosynthesis class F protein OS=Homo sapiens OX=9606...	[more]
Q6C741	1.6e-11	31.36	Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Yarrowia lipolyti...	[more]
P0C148	2.1e-11	35.34	Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Magnaporthe oryza...	[more]

BLAST of CsaV3_3G032650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9Y3	1.4e-125	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710220 PE=3 SV=1	[more]
A0A5A7T0F5	1.3e-120	96.44	Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Cucumis melo var....	[more]
A0A6J1KCP2	6.1e-102	81.86	phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita maxima OX=...	[more]
A0A6J1GAT8	6.1e-102	82.30	phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita moschata O...	[more]
A0A6J1DKK8	9.1e-98	79.73	phosphatidylinositol-glycan biosynthesis class F protein OS=Momordica charantia ...	[more]

BLAST of CsaV3_3G032650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G16040.1	5.9e-65	54.34	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic p...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009580	GPI biosynthesis protein Pig-F	PFAM	PF06699	PIG-F	coord: 39..210 e-value: 4.2E-40 score: 137.8
None	No IPR available	PANTHER	PTHR43157:SF41	BNAA09G56460D PROTEIN	coord: 10..217
None	No IPR available	PANTHER	PTHR43157	PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED	coord: 10..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_3G032650.1	CsaV3_3G032650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006506	GPI anchor biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane