CsaV3_3G030180 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_3G030180
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description(1->3)-beta-glucan endohydrolase
Locationchr3: 25905804 .. 25908181 (-)
RNA-Seq ExpressionCsaV3_3G030180
SyntenyCsaV3_3G030180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTAAGACATAAATATAATTATAAATTTAAAAGGGTATAGTGTATTAAATTTGATATGATATTTTTACAATTTTGATGTGGAACATAAAGCCCTAATATCTGTCAATTAATCCAACGGAGGTTTTTTATTTTATTTTTTTAGTTAGGGTTTGCTTATCAAAAATAAAGTAAATTATTCCAATTTTGTCCTCCACGAGTCATTGACTCTCAAGTCCCACATCTACGTCTCGGTCTCCTATATCACCATCACCATCATCCTCCATTAATCCCCACTCTCTCCATTTCCTCCCATTCTTCCTCTTCCTCTTCTTCTTCTTCTGCAACAATGCCCATTCCCTTCCTTTCTCTACTCTTCTTTCTCTTTTTCCTCTCACTCTCAAGTAAACTCCAATATCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACGTTTTTGTATTTCTCTCTAACCTTCTCCTCCTCATTTCTTGCAGGTGCAGAAATCTCAACCCTCGTCGGAATCAACTACGGTCAGCTCGGCAACAACCTCCCTCCCCCCTCCCATTCCGTCAATTTAATCAAATCCCTAAACGCTCAAATCGTCAAAATCTACGACGCAAATCCCCAAATCCTCAAAGCTCTCAAAAACACCGATCTACGGGTCTCCGTCATGGTTCCTAACGAACTCATCATCAACATTTCTTCTAGCCAGAACCTCGCCGACCAATGGGTTCGGACCAATATCCTCCCCTTTTACCCCCAAACCAAAATCCGTTACCTTCTCGTCGGAAACGAAATCATCTCCTCCACTGGAAATCAAACCTGGTTCAGTTTAGTACCGGCGATGCGCCGAATCAAACATTCTTTGAAAACTTACGGGATTCATAAAGTGAAAGTCGGAACACCCTCTGCTATGGATGTTCTTCAGTCGTCTTTTCCGCCCTCGAATGGGACATTCCGGGCGGATATTGCGGATCGTGTCATGAGACCGATGTTGCAGTTTCTTAACCGGACGAAATCGTTCTTCTTCCTTGATGTGTATCCCTATTTCCCTTGGTCTTCCGATTCAGTTAACATCAAACTCGATTATGCTCTGTTTGAATCGAAGAACATTACGTATTCGGATCCGGTTTCGGGCTTGGTTTACACGAATCTCTTCGATCAAATGGTGGATTCAGTGATTTTCGCTATGAAACGTCTCGGCTTCCCCGATATCCGAGTATTCATCGCCGAAACCGGATGGCCAAACGGCGGCGATTACGACCAAATCGGCGCCAGCATCCATAATGCCGCCGTGTACAACCGGAACATCGTGAAACGAGTCACTTCGAAACCGCCAGTTGGTACACCGGCTCGACCGGGACGGATGTTGCCAACGATAATATTCTCTTTGTATAATGAGAATCAGAAACCGGGTCCGGGTACGGAGAGGCATTTCGGACTGTTGTATCCGAAGGGAAAGGCGGTTTATGAGATGGATTTGAGTGGGTCGAGAGCGGAGACAAAGTTTAAGCCGCTGCCAGAGGCGGAGAGGAATGAACCGTACAAGGGGAAGATTTGGTGCGTAGTTGGGAAGGGCGTGAATATGAGTGATGTTGTTGGGGCTTTATCGTACGCATGCTCCCAGGGAAATAAAACGTGTGAACCGATACGAAAGGGCGGTCCGTGTTATGAACCGGATTCCTTAAAACGACATGCAAGTTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTCGGTGGAACCTGTTACTTCAATGGCTTAGCAACTCAAACCATCAAAGACCCAAGTAAGTTTTTGTTTTTGTTATTATTTTTTTCTTAACTAAAACACATATCAATTATTCTTTACAAGATTTAGTAACACGTTTTTATTTTCTTTGCATTTCAAACCATTGGGGTTGTTTGAAATTTATTGTTTTTTGAGAGTAGAATTTAATTAGTGGTATTGGTGAACTAAAGAAAAACAATTAATTTCTTGCAGGTTATGGAAAGTGCAAGTTCCCAAGTGTGACATTGCAAGCATCATAAGTTATGAATTTGATGGGTGAATTATAAGAAAGGTGGTTTGGGCATCCCATTTCCTTCTCTTTTTGTTTTTAATTTTAAATATTAATCAAGGAAGGACCAAAATGGAAAAGTGGGGGTTTTTTTATTGATAATTTTAATTTGATGGTGAGAATTGAAATGGGGTGCCCAAACCAGCAATAGCATCGCCAAATATGATTCAGTAATTCCACCCTCCTTTTTGCTCTGTCTTTTTCATCAACGCGTGGTCCACTTTTTCAACGCCCGTTTTTATTTATGCTACACCTAACGGCTACTTTTCTTTTCTCTTTTACTCTTTTCTCTTTTAATTATTATGTATTTTGTTTGGCAAATTAATATCATTATTTTT

mRNA sequence

ATGCCCATTCCCTTCCTTTCTCTACTCTTCTTTCTCTTTTTCCTCTCACTCTCAAGTGCAGAAATCTCAACCCTCGTCGGAATCAACTACGGTCAGCTCGGCAACAACCTCCCTCCCCCCTCCCATTCCGTCAATTTAATCAAATCCCTAAACGCTCAAATCGTCAAAATCTACGACGCAAATCCCCAAATCCTCAAAGCTCTCAAAAACACCGATCTACGGGTCTCCGTCATGGTTCCTAACGAACTCATCATCAACATTTCTTCTAGCCAGAACCTCGCCGACCAATGGGTTCGGACCAATATCCTCCCCTTTTACCCCCAAACCAAAATCCGTTACCTTCTCGTCGGAAACGAAATCATCTCCTCCACTGGAAATCAAACCTGGTTCAGTTTAGTACCGGCGATGCGCCGAATCAAACATTCTTTGAAAACTTACGGGATTCATAAAGTGAAAGTCGGAACACCCTCTGCTATGGATGTTCTTCAGTCGTCTTTTCCGCCCTCGAATGGGACATTCCGGGCGGATATTGCGGATCGTGTCATGAGACCGATGTTGCAGTTTCTTAACCGGACGAAATCGTTCTTCTTCCTTGATGTGTATCCCTATTTCCCTTGGTCTTCCGATTCAGTTAACATCAAACTCGATTATGCTCTGTTTGAATCGAAGAACATTACGTATTCGGATCCGGTTTCGGGCTTGGTTTACACGAATCTCTTCGATCAAATGGTGGATTCAGTGATTTTCGCTATGAAACGTCTCGGCTTCCCCGATATCCGAGTATTCATCGCCGAAACCGGATGGCCAAACGGCGGCGATTACGACCAAATCGGCGCCAGCATCCATAATGCCGCCGTGTACAACCGGAACATCGTGAAACGAGTCACTTCGAAACCGCCAGTTGGTACACCGGCTCGACCGGGACGGATGTTGCCAACGATAATATTCTCTTTGTATAATGAGAATCAGAAACCGGGTCCGGGTACGGAGAGGCATTTCGGACTGTTGTATCCGAAGGGAAAGGCGGTTTATGAGATGGATTTGAGTGGGTCGAGAGCGGAGACAAAGTTTAAGCCGCTGCCAGAGGCGGAGAGGAATGAACCGTACAAGGGGAAGATTTGGTGCGTAGTTGGGAAGGGCGTGAATATGAGTGATGTTGTTGGGGCTTTATCGTACGCATGCTCCCAGGGAAATAAAACGTGTGAACCGATACGAAAGGGCGGTCCGTGTTATGAACCGGATTCCTTAAAACGACATGCAAGTTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTCGGTGGAACCTGTTACTTCAATGGCTTAGCAACTCAAACCATCAAAGACCCAAGTTATGGAAAGTGCAAGTTCCCAAGTGTGACATTGCAAGCATCATAA

Coding sequence (CDS)

ATGCCCATTCCCTTCCTTTCTCTACTCTTCTTTCTCTTTTTCCTCTCACTCTCAAGTGCAGAAATCTCAACCCTCGTCGGAATCAACTACGGTCAGCTCGGCAACAACCTCCCTCCCCCCTCCCATTCCGTCAATTTAATCAAATCCCTAAACGCTCAAATCGTCAAAATCTACGACGCAAATCCCCAAATCCTCAAAGCTCTCAAAAACACCGATCTACGGGTCTCCGTCATGGTTCCTAACGAACTCATCATCAACATTTCTTCTAGCCAGAACCTCGCCGACCAATGGGTTCGGACCAATATCCTCCCCTTTTACCCCCAAACCAAAATCCGTTACCTTCTCGTCGGAAACGAAATCATCTCCTCCACTGGAAATCAAACCTGGTTCAGTTTAGTACCGGCGATGCGCCGAATCAAACATTCTTTGAAAACTTACGGGATTCATAAAGTGAAAGTCGGAACACCCTCTGCTATGGATGTTCTTCAGTCGTCTTTTCCGCCCTCGAATGGGACATTCCGGGCGGATATTGCGGATCGTGTCATGAGACCGATGTTGCAGTTTCTTAACCGGACGAAATCGTTCTTCTTCCTTGATGTGTATCCCTATTTCCCTTGGTCTTCCGATTCAGTTAACATCAAACTCGATTATGCTCTGTTTGAATCGAAGAACATTACGTATTCGGATCCGGTTTCGGGCTTGGTTTACACGAATCTCTTCGATCAAATGGTGGATTCAGTGATTTTCGCTATGAAACGTCTCGGCTTCCCCGATATCCGAGTATTCATCGCCGAAACCGGATGGCCAAACGGCGGCGATTACGACCAAATCGGCGCCAGCATCCATAATGCCGCCGTGTACAACCGGAACATCGTGAAACGAGTCACTTCGAAACCGCCAGTTGGTACACCGGCTCGACCGGGACGGATGTTGCCAACGATAATATTCTCTTTGTATAATGAGAATCAGAAACCGGGTCCGGGTACGGAGAGGCATTTCGGACTGTTGTATCCGAAGGGAAAGGCGGTTTATGAGATGGATTTGAGTGGGTCGAGAGCGGAGACAAAGTTTAAGCCGCTGCCAGAGGCGGAGAGGAATGAACCGTACAAGGGGAAGATTTGGTGCGTAGTTGGGAAGGGCGTGAATATGAGTGATGTTGTTGGGGCTTTATCGTACGCATGCTCCCAGGGAAATAAAACGTGTGAACCGATACGAAAGGGCGGTCCGTGTTATGAACCGGATTCCTTAAAACGACATGCAAGTTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTCGGTGGAACCTGTTACTTCAATGGCTTAGCAACTCAAACCATCAAAGACCCAAGTTATGGAAAGTGCAAGTTCCCAAGTGTGACATTGCAAGCATCATAA

Protein sequence

MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDANPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPPVGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPLPEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQAS*
Homology
BLAST of CsaV3_3G030180 vs. NCBI nr
Match: XP_004144397.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucumis sativus] >KGN58384.1 hypothetical protein Csa_001975 [Cucumis sativus])

HSP 1 Score: 951.8 bits (2459), Expect = 2.2e-273
Identity = 466/466 (100.00%), Postives = 466/466 (100.00%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA
Sbjct: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI
Sbjct: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF
Sbjct: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300

Query: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360
           VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL
Sbjct: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQAS 467
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQAS
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQAS 466

BLAST of CsaV3_3G030180 vs. NCBI nr
Match: XP_008460404.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase A6 [Cucumis melo] >KAA0068092.1 putative glucan endo-1,3-beta-glucosidase A6 [Cucumis melo var. makuwa])

HSP 1 Score: 909.4 bits (2349), Expect = 1.2e-260
Identity = 443/464 (95.47%), Postives = 453/464 (97.63%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           MPIPFLSLL F FFLSLSSAEIS+LVGINYGQLGNNLP PSHSVNLIKSLNAQIVKIYDA
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQIL ALKNTDLRVSVMVPNELIINISS+QNLADQWVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGT SAMDVLQSSFPPSNGTFR DIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VMRPMLQFLNRTKSF+FLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKR+TSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360
           VGTPARPGR+LPTIIFSLYNEN+K GPGTERHFGLLYP GKAVYEMDLSGS AETKFKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           PEAE NEPYKG+IWCVVGKGVNMS+VVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

BLAST of CsaV3_3G030180 vs. NCBI nr
Match: XP_038886980.1 (probable glucan endo-1,3-beta-glucosidase A6 [Benincasa hispida])

HSP 1 Score: 881.3 bits (2276), Expect = 3.6e-252
Identity = 429/464 (92.46%), Postives = 442/464 (95.26%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           M IPFLSLL   FFLSLSSAEIS+LVGINYGQLGNNLPPPS SVNLIKSLNAQIVKIYDA
Sbjct: 1   MAIPFLSLLLPFFFLSLSSAEISSLVGINYGQLGNNLPPPSDSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILK LKNTDLRVSVMVPN+LIINISS+QNLAD WVRTN+ PFYPQTKIRYLLVGNEI
Sbjct: 61  NPQILKTLKNTDLRVSVMVPNDLIINISSNQNLADDWVRTNVRPFYPQTKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           +SSTGN+TWFSLVPAMRRIK SLKTYGI KVKVGT SAMDVLQSSFPPSNGTFRADIA+R
Sbjct: 121 VSSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIAER 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYPYFPW+ DSVNIKLDYALFESKNITYSDP SGL+YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPYFPWAIDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPD+RVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDVRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300

Query: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360
           VGTPARPGR+LPTIIFSLYNENQKPGPGTERHFGLLYP   AVYEMDLSGS  ET+FKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENQKPGPGTERHFGLLYPNRTAVYEMDLSGSTPETEFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           P AE NEPYKGKIWCVVGKG NMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PAAETNEPYKGKIWCVVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSV LQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVVLQ 464

BLAST of CsaV3_3G030180 vs. NCBI nr
Match: XP_022931078.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita moschata])

HSP 1 Score: 834.3 bits (2154), Expect = 5.0e-238
Identity = 403/464 (86.85%), Postives = 429/464 (92.46%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           M IPF SLL   +FLSL+SA+ISTLVGINYGQLGNNLPPP  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILK+L N+D++VSVMVPN+LIINISS+QNLAD WVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTW SLVPAMRRIK SLKTYGIHKVKVGT SAMDVLQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLG+PDIR+FIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360
           VGTPARPG +LPTIIFSLYNENQKPG GTERHFGLLYP   AVYEMDLSG   E  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           P AE NEPYKGKIWC+V +G N S+V GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP+YGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPNYGKCKFPSVTLQ 464

BLAST of CsaV3_3G030180 vs. NCBI nr
Match: XP_023532592.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833.2 bits (2151), Expect = 1.1e-237
Identity = 403/464 (86.85%), Postives = 428/464 (92.24%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           M IPF SLL   +FLSL+SA+ISTLVGINYGQLGNNLPPP  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILK+L N+D++VSVMVPN+LIINISS+QNLAD WVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTW SLVPAMRRIK SLK YGIHKVKVGT SAMDVLQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLG+PDIR+FIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360
           VGTPARPG +LPTIIFSLYNENQKPG GTERHFGLLYP   AVYEMDLSG   E  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           P AE NEPYKGKIWC+V +G N S+V GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

BLAST of CsaV3_3G030180 vs. ExPASy Swiss-Prot
Match: Q06915 (Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=A6 PE=2 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 1.4e-150
Identity = 244/443 (55.08%), Postives = 331/443 (74.72%), Query Frame = 0

Query: 21  EISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDANPQILKALKNTDLRVSVMVP 80
           ++++ +GINYG+ GNNLP P  S+N IKS+ A  VK+YDA+P+ L  L  T+L V++ VP
Sbjct: 37  DLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTNLYVTITVP 96

Query: 81  NELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTGNQTWFSLVPAMRRIK 140
           N  I  +SS+Q +AD+WVRTNILP+YPQT+IR++LVGNEI+S        +LVPAMR+I 
Sbjct: 97  NHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNLVPAMRKIV 156

Query: 141 HSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPMLQFLNRTKSFFFLDV 200
           +SL+ +GIH +KVGTP AMD L+SSFPPSNGTFR +I   VM P+L+FLN T S+FFL+V
Sbjct: 157 NSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGTNSYFFLNV 216

Query: 201 YPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVDSVIFAMKRLGFPDIR 260
           +PYF WS + +N  LD+ALF+  + TY+DP +GLVY NL DQM+DSV+FAM +LG+P +R
Sbjct: 217 HPYFRWSRNPMNTSLDFALFQGHS-TYTDPQTGLVYRNLLDQMLDSVLFAMTKLGYPHMR 276

Query: 261 VFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPPVGTPARPGRMLPTIIFSLYN 320
           + I+ETGWPN GD D+ GA+I NAA YNRN++K++++ PP+GTP+RPG  +PT +FSL+N
Sbjct: 277 LAISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFN 336

Query: 321 ENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPLPEAERNEPYKGKIWCVVGKG 380
           ENQK G GT+RH+G+L+P G  +Y++D +G    T F PLP+   N PYKG++WCV  +G
Sbjct: 337 ENQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQVWCVPVEG 396

Query: 381 VNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYF 440
            N +++   L  AC+Q N TC  +  G  CYEP S+  HAS+A +SYWAQFR     C+F
Sbjct: 397 ANETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFRNQSIQCFF 456

Query: 441 NGLATQTIKDPSYGKCKFPSVTL 464
           NGLA +T  +P   +CKFPSVTL
Sbjct: 457 NGLAHETTTNPGNDRCKFPSVTL 478

BLAST of CsaV3_3G030180 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 7.4e-91
Identity = 172/433 (39.72%), Postives = 251/433 (57.97%), Query Frame = 0

Query: 26  VGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDANPQILKALKNTDLRVSVMVPNELII 85
           VG+ YG+  ++LP PS  V LI+  N + V+IYD N Q+LKA  NT + + + VPN  + 
Sbjct: 26  VGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDLN 85

Query: 86  NISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTGNQTWFSLVPAMRRIKHSLKT 145
             S SQ+  D W++ ++LP+YP TKI Y+ VG E            +VPAM+ +  +L+ 
Sbjct: 86  AFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLTALRK 145

Query: 146 YGI-HKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPMLQFLNRTKSFFFLDVYPYF 205
            G+  ++KV T  ++ +L  SFPPS G F +  A   +RPML+FL   KS F +D+YPY+
Sbjct: 146 VGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYA-YFLRPMLEFLAENKSPFMIDLYPYY 205

Query: 206 PWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVDSVIFAMKRLGFPDIRVFIA 265
            +     N+ LDY LFES +    DP +GL+Y N+FD  VD++ +A+  L F  I++ + 
Sbjct: 206 AYRDSPNNVSLDYVLFESSS-EVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMVT 265

Query: 266 ETGWP-NGGDYDQIGASIHNAAVYNRNIVKRVTSKPPVGTPARPGRMLPTIIFSLYNENQ 325
           ETGWP  G   ++  AS  NA  YN NI++ V +    GTPA+PG  +   IFSL+NEN+
Sbjct: 266 ETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQ--GTPAKPGEAMNVYIFSLFNENR 325

Query: 326 KPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPLPEAERNEPYKGKIWCVVGKGVNM 385
           K G  +ER++GL YP   +VY++D +G   ++       +  N       WC+     + 
Sbjct: 326 KAGLDSERNWGLFYPDQTSVYQLDFTG---KSNGFHSNSSGTNSSGSSNSWCIASSKASE 385

Query: 386 SDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGL 445
            D+ GAL +AC  GN  C  I+   PC++PD+L  HASF F+SY+ Q R     C F G 
Sbjct: 386 RDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGA 445

Query: 446 ATQTIKDPSYGKC 457
             +  KDPSY KC
Sbjct: 446 GVKVNKDPSYDKC 451

BLAST of CsaV3_3G030180 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.5e-88
Identity = 174/457 (38.07%), Postives = 267/457 (58.42%), Query Frame = 0

Query: 7   SLLFFLFFL--SLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDANPQI 66
           SLLF +F L  +  SA   + +G+NYGQL +NLP    +VNL+KS   Q V+++ A P +
Sbjct: 9   SLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAV 68

Query: 67  LKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISST 126
           +KA  NT + + +   N  I  ++S+ N+A Q+V++N++ FYP + I  + VGNE+++S 
Sbjct: 69  IKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSG 128

Query: 127 GNQTWFSLVPAMRRIKHSLKTYGI-HKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMR 186
             +    L+PAM+ ++++L    +  KVKV T  AM VL  S+PPS+G F   + D  M+
Sbjct: 129 DQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGD-TMK 188

Query: 187 PMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQM 246
            +LQF +   + F +  YPYF + +      L + LF+  N    D  +G  YTN+FD  
Sbjct: 189 ALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQ-PNAGQVDSGNGHKYTNMFDAQ 248

Query: 247 VDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPPVGT 306
           VD+V  A+  +GF DI + +AETGWP+GGD +++G S+ NA  Y  N++  + SK  VGT
Sbjct: 249 VDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSK--VGT 308

Query: 307 PARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPLPEA 366
           P  PG+ + T +FSLY+E++K G  +E++FGL  P G   Y++   G    T+    P  
Sbjct: 309 PLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDV---GLLKNTQNPTTPAT 368

Query: 367 ERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFA 426
               P     WCV   GV+   + G ++YAC QG   C PI+ GG C+EP+++K HA++ 
Sbjct: 369 PTPTPKAAGSWCVPKPGVSDDQLTGNINYACGQG-IDCGPIQPGGACFEPNTVKAHAAYV 428

Query: 427 FSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
            + Y+    +    C F+  AT T  +PSYG C FPS
Sbjct: 429 MNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFPS 457

BLAST of CsaV3_3G030180 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.5e-88
Identity = 169/477 (35.43%), Postives = 272/477 (57.02%), Query Frame = 0

Query: 6   LSLLFFLF--FLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDANPQ 65
           L+L+F++F   L+  +  +++ +GI YG+  +NLP P+    LI+ LN + V+IYDAN  
Sbjct: 5   LNLVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANID 64

Query: 66  ILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISS 125
           +LKA  NT + + + VPN  ++  +  Q+  D W+  NILP+YP TKI  + VG E+  +
Sbjct: 65  VLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEA 124

Query: 126 TGNQTWFSLVPAMRRIKHSLKTYGI-HKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVM 185
             N T   ++PAMR I  +LK  G+  K+K+ +  ++ +L  SFPPS+ +F +      +
Sbjct: 125 PDNATGL-VLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASF-SKKHSAFL 184

Query: 186 RPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQ 245
           +PML+FL   +S F +D+YPY+ +   +  + L+YALFES +    DP +GL+Y+N+FD 
Sbjct: 185 KPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSS-QVVDPATGLLYSNMFDA 244

Query: 246 MVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPPVG 305
            +D++ FA+  + F  ++V + E+GWP+ G   +  A+  NA  YN N+++ V   P  G
Sbjct: 245 QLDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDP--G 304

Query: 306 TPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPLPE 365
           TPA+PG  +   +FSL+NEN+KPG  +ER++G+ Y  G  VY +D +G    T   P   
Sbjct: 305 TPAKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENT-TPVSPTNS 364

Query: 366 AERNEPYKG---------------------KIWCVVGKGVNMSDVVGALSYACSQGNKTC 425
                P                        K WC+     +++++  AL +AC  GN  C
Sbjct: 365 TTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDC 424

Query: 426 EPIRKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKF 459
             ++   PC+EPD++  HAS+AF++Y+ Q       C FNG + +  KDPSYG C +
Sbjct: 425 SAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of CsaV3_3G030180 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 323.6 bits (828), Expect = 3.8e-87
Identity = 177/460 (38.48%), Postives = 273/460 (59.35%), Query Frame = 0

Query: 6   LSLLFFLFFLS-LSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDANPQI 65
           +S+ F L FLS   S+     +G+NYGQ+ +NLPPPS +V L++S + Q V++Y A+P I
Sbjct: 5   ISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAI 64

Query: 66  LKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISST 125
           +KAL  T + + +   N  + +++S  N A QW+ +N+LPFYP +KI  + VGNEI+ S 
Sbjct: 65  IKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSN 124

Query: 126 GNQTWFSLVPAMRRIKHSLKTYGI-HKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMR 185
                  L+PAM+ ++ +L+   +  K+KV T ++M VL SS PPS+G+F A      ++
Sbjct: 125 DPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGY-QTGLK 184

Query: 186 PMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQM 245
            +LQFL+ T S F ++ YP+F + SD     L + LFE  N    D  +G+ YTN+FD  
Sbjct: 185 GILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFE-PNAGRVDSKTGIKYTNMFDAQ 244

Query: 246 VDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPPVGT 305
           VD+V  A+K +GF  + + +AETGW + GD +++GAS+ NA  YN N++  + S   VGT
Sbjct: 245 VDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSM--VGT 304

Query: 306 PARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPLPEA 365
           P  PG+ + T IF+LY+EN KPGP +ER FGL       VY++ L+ S           +
Sbjct: 305 PLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKS-----------S 364

Query: 366 ERNEPYKGKI----WCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRH 425
             ++   GK+    WCV  KG    ++  +L +AC  G   C  I+ GG C+EP+++  H
Sbjct: 365 SSSQTPSGKVTSSGWCVPKKGATNEELQASLDWACGHG-IDCGAIQPGGACFEPNNVVSH 424

Query: 426 ASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFP 460
           A++A + Y+ +  K    C F+  AT T ++PSY  C +P
Sbjct: 425 AAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

BLAST of CsaV3_3G030180 vs. ExPASy TrEMBL
Match: A0A0A0LBR1 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G634360 PE=3 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.1e-273
Identity = 466/466 (100.00%), Postives = 466/466 (100.00%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA
Sbjct: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI
Sbjct: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF
Sbjct: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300

Query: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360
           VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL
Sbjct: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQAS 467
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQAS
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQAS 466

BLAST of CsaV3_3G030180 vs. ExPASy TrEMBL
Match: A0A5A7VIC4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G00460 PE=3 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 6.0e-261
Identity = 443/464 (95.47%), Postives = 453/464 (97.63%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           MPIPFLSLL F FFLSLSSAEIS+LVGINYGQLGNNLP PSHSVNLIKSLNAQIVKIYDA
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQIL ALKNTDLRVSVMVPNELIINISS+QNLADQWVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGT SAMDVLQSSFPPSNGTFR DIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VMRPMLQFLNRTKSF+FLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKR+TSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360
           VGTPARPGR+LPTIIFSLYNEN+K GPGTERHFGLLYP GKAVYEMDLSGS AETKFKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           PEAE NEPYKG+IWCVVGKGVNMS+VVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

BLAST of CsaV3_3G030180 vs. ExPASy TrEMBL
Match: A0A1S3CCE3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103499229 PE=3 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 6.0e-261
Identity = 443/464 (95.47%), Postives = 453/464 (97.63%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           MPIPFLSLL F FFLSLSSAEIS+LVGINYGQLGNNLP PSHSVNLIKSLNAQIVKIYDA
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQIL ALKNTDLRVSVMVPNELIINISS+QNLADQWVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGT SAMDVLQSSFPPSNGTFR DIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VMRPMLQFLNRTKSF+FLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKR+TSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360
           VGTPARPGR+LPTIIFSLYNEN+K GPGTERHFGLLYP GKAVYEMDLSGS AETKFKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           PEAE NEPYKG+IWCVVGKGVNMS+VVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

BLAST of CsaV3_3G030180 vs. ExPASy TrEMBL
Match: A0A6J1EXC8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437372 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.4e-238
Identity = 403/464 (86.85%), Postives = 429/464 (92.46%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           M IPF SLL   +FLSL+SA+ISTLVGINYGQLGNNLPPP  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILK+L N+D++VSVMVPN+LIINISS+QNLAD WVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTW SLVPAMRRIK SLKTYGIHKVKVGT SAMDVLQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLG+PDIR+FIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360
           VGTPARPG +LPTIIFSLYNENQKPG GTERHFGLLYP   AVYEMDLSG   E  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           P AE NEPYKGKIWC+V +G N S+V GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP+YGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPNYGKCKFPSVTLQ 464

BLAST of CsaV3_3G030180 vs. ExPASy TrEMBL
Match: A0A6J1K875 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491531 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 5.1e-236
Identity = 399/464 (85.99%), Postives = 427/464 (92.03%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           M IPF SLL   +FLSL+SA+ISTLVGINYGQLGNNLPPP  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           NPQILK+L N+D++VSVMVPN+LIINISS+QNLAD WVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGNQTW SLVPAMRRIK SLK YGIHKVKVG+ SAMD+LQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGSSSAMDILQSSFPPSNGTFRADISDR 180

Query: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLG+PDIRVFI+ETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRVFISETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360
           VGTPARPG +LPTIIFSLYNENQKPG GTERHFGLLYP   AVYEMDLSG   E  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           P AE NEP+KGKIWC+V +G N S+V GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPFKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKC FPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCMFPSVTLQ 464

BLAST of CsaV3_3G030180 vs. TAIR 10
Match: AT3G07320.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 658.3 bits (1697), Expect = 4.6e-189
Identity = 315/463 (68.03%), Postives = 379/463 (81.86%), Query Frame = 0

Query: 6   LSLLFFLFFLSL----SSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDAN 65
           +SLL  LF LSL    S A+ S   GINYGQLGNNLP PS SVNLIKSLNA+ VK+YDAN
Sbjct: 1   MSLLLHLFALSLLISVSGAKFSGRPGINYGQLGNNLPSPSDSVNLIKSLNAKRVKLYDAN 60

Query: 66  PQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEII 125
           P+IL AL  TD+ VSVMVPNEL++NIS S +L+D W+R+NILPFYP TKIRYLLVGNEI+
Sbjct: 61  PKILAALNGTDITVSVMVPNELLVNISKSASLSDDWIRSNILPFYPTTKIRYLLVGNEIL 120

Query: 126 SSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADRV 185
           S   ++   SLVPAMR+I+ SLK+ G+ KVKVGT  A DVLQSSFPPS+G FR DI+  +
Sbjct: 121 SLPDSELKSSLVPAMRKIQRSLKSLGVKKVKVGTTLATDVLQSSFPPSSGEFREDISGLI 180

Query: 186 MRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFD 245
           M+PMLQFLNRTKSF F+DVYPYF W+ D  ++ LDYA+FES N+T +DPVS L Y NLFD
Sbjct: 181 MKPMLQFLNRTKSFLFVDVYPYFAWAQDPTHVDLDYAIFESTNVTVTDPVSNLTYHNLFD 240

Query: 246 QMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPPV 305
           QM+D+ +FAMKR+G+PDIR+++AETGWPN GDYDQIGA+I+NAA YNRN+VK++ + PPV
Sbjct: 241 QMIDAFVFAMKRVGYPDIRIWVAETGWPNNGDYDQIGANIYNAATYNRNVVKKLAADPPV 300

Query: 306 GTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFK-PL 365
           GTPARPG++LP  +F+LYNENQK GPGTERHFGLL+P G  VY +DLSG   +T++K  L
Sbjct: 301 GTPARPGKVLPAFVFALYNENQKTGPGTERHFGLLHPNGTQVYGIDLSG---KTEYKESL 360

Query: 366 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 425
           P  E N+ YKGKIWCVV KG N + +  ALSYACSQGN TC+PI++GGPC +PD    HA
Sbjct: 361 PAPENNDLYKGKIWCVVAKGANWTQLGDALSYACSQGNNTCDPIQRGGPCQKPDLTVLHA 420

Query: 426 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTL 464
           S+AFSSYWAQFRK+GGTC FNGLATQTIKDPSYG+C+FPSVTL
Sbjct: 421 SYAFSSYWAQFRKIGGTCSFNGLATQTIKDPSYGRCEFPSVTL 460

BLAST of CsaV3_3G030180 vs. TAIR 10
Match: AT3G23770.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-157
Identity = 267/475 (56.21%), Postives = 347/475 (73.05%), Query Frame = 0

Query: 4   PFLSLLFFLFFLSLSSAE------------ISTLVGINYGQLGNNLPPPSHSVNLIKSLN 63
           PF   LF L  LS S               +++ +GINYG+LGNNLP P  S+NLIK++ 
Sbjct: 3   PFALFLFTLLALSSSCCSAIGPQNNRTVLALASRIGINYGKLGNNLPFPYQSINLIKTIK 62

Query: 64  AQIVKIYDANPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKI 123
           A  VK+YDA+P+ LK L  T+L V++MVPN  II+I + Q  AD WV TN+LPF+PQT+I
Sbjct: 63  AGHVKLYDADPETLKLLSTTNLYVTIMVPNNQIISIGADQAAADNWVATNVLPFHPQTRI 122

Query: 124 RYLLVGNEIISSTGN---QTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPP 183
           R++LVGNE++S + +   Q W +LVPAMR++ +SL+  GIH +KVGTP AMD L+SSFPP
Sbjct: 123 RFVLVGNEVLSYSSDQDKQIWANLVPAMRKVVNSLRARGIHNIKVGTPLAMDALRSSFPP 182

Query: 184 SNGTFRADIADRVMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYS 243
           S+GTFR DIA  VM P+L+FLN T SFFFLDVYPYFPWS+D VN  LD+ALFES N TY+
Sbjct: 183 SSGTFREDIAVPVMLPLLKFLNGTNSFFFLDVYPYFPWSTDPVNNHLDFALFES-NSTYT 242

Query: 244 DPVSGLVYTNLFDQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYN 303
           DP +GLVYTNL DQM+DSVIFAM +LG+P+I + I+ETGWPN GD  + GA+I NAA YN
Sbjct: 243 DPQTGLVYTNLLDQMLDSVIFAMTKLGYPNISLAISETGWPNDGDIHETGANIVNAATYN 302

Query: 304 RNIVKRVTSKPPVGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDL 363
           RN++K++T+ PP+GTPAR G  +PT +FSL+NENQKPG GTERH+G+L P G  +Y++D 
Sbjct: 303 RNLIKKMTANPPLGTPARRGAPIPTFLFSLFNENQKPGSGTERHWGILNPDGTPIYDIDF 362

Query: 364 SGSRAETKFKPLPEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGG 423
           SG R+ + F  LP+   N P+KG +WCV   G + +++  AL++AC + N TC  +  GG
Sbjct: 363 SGRRSFSGFDSLPKPSNNVPFKGNVWCVAVDGADEAELGQALNFACGRSNATCAALAPGG 422

Query: 424 PCYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTL 464
            CY P ++  HAS+AFSSYWAQFR     CYFNGLA +T  +P   +CKFPSVTL
Sbjct: 423 ECYAPVTVTWHASYAFSSYWAQFRNQSSQCYFNGLARETTTNPGNERCKFPSVTL 476

BLAST of CsaV3_3G030180 vs. TAIR 10
Match: AT4G14080.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 534.3 bits (1375), Expect = 1.0e-151
Identity = 244/443 (55.08%), Postives = 331/443 (74.72%), Query Frame = 0

Query: 21  EISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDANPQILKALKNTDLRVSVMVP 80
           ++++ +GINYG+ GNNLP P  S+N IKS+ A  VK+YDA+P+ L  L  T+L V++ VP
Sbjct: 37  DLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTNLYVTITVP 96

Query: 81  NELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTGNQTWFSLVPAMRRIK 140
           N  I  +SS+Q +AD+WVRTNILP+YPQT+IR++LVGNEI+S        +LVPAMR+I 
Sbjct: 97  NHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNLVPAMRKIV 156

Query: 141 HSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPMLQFLNRTKSFFFLDV 200
           +SL+ +GIH +KVGTP AMD L+SSFPPSNGTFR +I   VM P+L+FLN T S+FFL+V
Sbjct: 157 NSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGTNSYFFLNV 216

Query: 201 YPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVDSVIFAMKRLGFPDIR 260
           +PYF WS + +N  LD+ALF+  + TY+DP +GLVY NL DQM+DSV+FAM +LG+P +R
Sbjct: 217 HPYFRWSRNPMNTSLDFALFQGHS-TYTDPQTGLVYRNLLDQMLDSVLFAMTKLGYPHMR 276

Query: 261 VFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPPVGTPARPGRMLPTIIFSLYN 320
           + I+ETGWPN GD D+ GA+I NAA YNRN++K++++ PP+GTP+RPG  +PT +FSL+N
Sbjct: 277 LAISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFN 336

Query: 321 ENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPLPEAERNEPYKGKIWCVVGKG 380
           ENQK G GT+RH+G+L+P G  +Y++D +G    T F PLP+   N PYKG++WCV  +G
Sbjct: 337 ENQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQVWCVPVEG 396

Query: 381 VNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYF 440
            N +++   L  AC+Q N TC  +  G  CYEP S+  HAS+A +SYWAQFR     C+F
Sbjct: 397 ANETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFRNQSIQCFF 456

Query: 441 NGLATQTIKDPSYGKCKFPSVTL 464
           NGLA +T  +P   +CKFPSVTL
Sbjct: 457 NGLAHETTTNPGNDRCKFPSVTL 478

BLAST of CsaV3_3G030180 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 365.9 bits (938), Expect = 4.7e-101
Identity = 188/463 (40.60%), Postives = 284/463 (61.34%), Query Frame = 0

Query: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60
           M + FL     L FLS   A  S +VG+NYG++ NNLP P   VNL+KS     +KI+D 
Sbjct: 1   MAVSFLPYFLILSFLSAIDAH-SGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDT 60

Query: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120
           +  +L AL N+ ++V V +PNEL+ + +S Q+ AD W++T+I+P++P T+I  + VGNE+
Sbjct: 61  DKNVLTALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEV 120

Query: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHK-VKVGTPSAMDVLQSSFPPSNGTFRADIAD 180
                   +  LV AM+ I  SL  Y + K +K+ +P A+  L +S+PPS+G+F+ ++ +
Sbjct: 121 FVDPTITPY--LVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIE 180

Query: 181 RVMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNL 240
            V++PML  L +T S+  ++ YP+F +++++  I LDYALF+ +N    D  +GL Y +L
Sbjct: 181 PVVKPMLALLQQTSSYLMVNAYPFFAYAANADKISLDYALFK-ENAGNIDSGTGLKYNSL 240

Query: 241 FDQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKP 300
           FD  +D+V  A+  +GF  ++V + ETGWP+ GD ++IGAS  NAA YN  +VKRV +  
Sbjct: 241 FDAQIDAVYAALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGK 300

Query: 301 PVGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKP 360
             GTP RP   L   +F+L+NENQKPGP +ER++GL YP    VY +  +    +    P
Sbjct: 301 --GTPLRPTEPLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFT----KKSTTP 360

Query: 361 LPEAERNEP--YKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLK 420
           +       P  ++G  WCV    V    +  AL YAC +G   C PI+ G  CY P+SL+
Sbjct: 361 VNGNRGKVPVTHEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPESLE 420

Query: 421 RHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
            HAS+AF+SY+ +  +  GTC+F G A    + P YGKC+FP+
Sbjct: 421 AHASYAFNSYYQKNSRRVGTCFFGGAAHVVTQPPRYGKCEFPT 453

BLAST of CsaV3_3G030180 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 361.7 bits (927), Expect = 8.9e-100
Identity = 182/468 (38.89%), Postives = 279/468 (59.62%), Query Frame = 0

Query: 8   LLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDANPQILKA 67
           L+F L  LS  S   +  +G+NYG++ + LP     V L+KS     VKI+DA+P +LKA
Sbjct: 7   LIFLLLLLSPFSLSDAGSIGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSVLKA 66

Query: 68  LKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEIISSTGNQ 127
           L  + ++V+V +PNEL+ + +   + A  WV+ N+  ++P T+I  + VGNE+   T N 
Sbjct: 67  LSGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDTHNT 126

Query: 128 TWFSLVPAMRRIKHSLKTYGIHK-VKVGTPSAMDVLQSSFPPSNGTFRADIADRVMRPML 187
           T F L+PAMR I  +L ++ +H  +K+ +P A+  LQ+S+P S+G+FR ++ D V++PML
Sbjct: 127 TSF-LIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIKPML 186

Query: 188 QFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLFDQMVDS 247
            FL  T S   ++VYP+F +  +S  I LDYAL   +N    D  +GL Y NLFD  +D+
Sbjct: 187 DFLRETGSRLMINVYPFFAYEGNSDVIPLDYALLR-ENPGMVDSGNGLRYFNLFDAQIDA 246

Query: 248 VIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPPVGTPAR 307
           V  AM  L + DI + + ETGWP+ GD +++GA++ NAA YN N+++R+ ++   GTP R
Sbjct: 247 VFAAMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRG--GTPLR 306

Query: 308 PGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSG--------------SR 367
           P   L   +F+L+NEN+K GP +ER++GL +P  K VY++  +               + 
Sbjct: 307 PKADLTVYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRDGGHTPVTG 366

Query: 368 AETKFKPLPEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYE 427
            +   KP      ++   G  WCV         + G L YAC +G   C PI+ G  CY 
Sbjct: 367 GDQVTKPPMSGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYS 426

Query: 428 PDSLKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
           PD+L+ HASFAF+SY+ +  + GG+CYF G A    + P YG+C+FP+
Sbjct: 427 PDTLEAHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPPKYGRCEFPT 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004144397.12.2e-273100.00probable glucan endo-1,3-beta-glucosidase A6 [Cucumis sativus] >KGN58384.1 hypot... [more]
XP_008460404.11.2e-26095.47PREDICTED: probable glucan endo-1,3-beta-glucosidase A6 [Cucumis melo] >KAA00680... [more]
XP_038886980.13.6e-25292.46probable glucan endo-1,3-beta-glucosidase A6 [Benincasa hispida][more]
XP_022931078.15.0e-23886.85probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita moschata][more]
XP_023532592.11.1e-23786.85probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q069151.4e-15055.08Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9FJU97.4e-9139.72Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Q94G861.5e-8838.07Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Q8VYE51.5e-8835.43Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9M0693.8e-8738.48Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LBR11.1e-273100.00(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G634360 PE=3... [more]
A0A5A7VIC46.0e-26195.47(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3CCE36.0e-26195.47(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103499229 PE=3 SV... [more]
A0A6J1EXC82.4e-23886.85(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437372 P... [more]
A0A6J1K8755.1e-23685.99(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491531 PE=... [more]
Match NameE-valueIdentityDescription
AT3G07320.14.6e-18968.03O-Glycosyl hydrolases family 17 protein [more]
AT3G23770.11.2e-15756.21O-Glycosyl hydrolases family 17 protein [more]
AT4G14080.11.0e-15155.08O-Glycosyl hydrolases family 17 protein [more]
AT4G26830.14.7e-10140.60O-Glycosyl hydrolases family 17 protein [more]
AT2G05790.18.9e-10038.89O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 373..458
e-value: 3.7E-40
score: 149.4
IPR012946X8 domainPFAMPF07983X8coord: 374..445
e-value: 4.2E-18
score: 65.8
NoneNo IPR availableGENE3D1.20.58.1040coord: 369..464
e-value: 1.2E-6
score: 30.7
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 25..356
e-value: 1.7E-119
score: 400.5
NoneNo IPR availablePANTHERPTHR32227:SF341BNAC03G35190D PROTEINcoord: 14..456
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 26..347
e-value: 1.0E-102
score: 343.9
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 259..272
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 14..456
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_3G030180.1CsaV3_3G030180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds