Homology
BLAST of CsaV3_3G028370 vs. NCBI nr
Match:
XP_004152001.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN58303.1 hypothetical protein Csa_017526 [Cucumis sativus])
HSP 1 Score: 834.7 bits (2155), Expect = 3.3e-238
Identity = 394/394 (100.00%), Postives = 394/394 (100.00%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV
Sbjct: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
BLAST of CsaV3_3G028370 vs. NCBI nr
Match:
XP_008447374.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo] >KAA0037954.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo var. makuwa])
HSP 1 Score: 798.5 bits (2061), Expect = 2.6e-227
Identity = 375/394 (95.18%), Postives = 385/394 (97.72%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRP WNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRT EI+NCEANV
Sbjct: 61 VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
+MLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFES+INKSSQRH+PPFTEDLPRLECPNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YYKQEDLL+EEIEQSQKKDLTWAVIRPN+IFGFSPFSLMN+VGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG KLAWEDFQ ASDADL+AEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IEEYGF EEGE LTLVDLMKDKSDVWDEIVKENQLQ TKLEEIG WW+VD IFS+SGNID
Sbjct: 301 IEEYGF-EEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of CsaV3_3G028370 vs. NCBI nr
Match:
XP_038899926.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Benincasa hispida])
HSP 1 Score: 763.5 bits (1970), Expect = 9.3e-217
Identity = 357/394 (90.61%), Postives = 375/394 (95.18%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
+ARRPRP WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWTNR TEIENCEANV
Sbjct: 61 IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GS +SI++KS Q HDPPFTEDLPRLE PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY QEDLL+EEIEQSQKKDLTWAVIRPN+IFGFSPFSL+N+VGTLCVYAAICKHEGR LK
Sbjct: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG+K AWEDFQV SDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IE+YGF E+GE LTLV+LMKDKS+VWDEIVKENQLQ TKLEEI WWF D IFS+SGNID
Sbjct: 301 IEDYGF-EDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of CsaV3_3G028370 vs. NCBI nr
Match:
XP_023535925.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 755.7 bits (1950), Expect = 1.9e-214
Identity = 350/394 (88.83%), Postives = 373/394 (94.67%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARR RP+WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+TE+ENCEANV
Sbjct: 61 VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESI+NKS QRHD PFTEDLPRL+CPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQRHDSPFTEDLPRLKCPNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY QEDLL+EEIE++Q+KDLTWAVIRPN+IFGFSPFS MN++GTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKTQRKDLTWAVIRPNVIFGFSPFSWMNIIGTLCAYAAICKHEGRPLK 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG K AWE+F +ASDADLIAEQHIWT+VDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEEFSIASDADLIAEQHIWTSVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IE+YGF EEGE LTLV+LMKDKS VW+EIVKENQLQ T+LEEIG WWFVD I S+SGNID
Sbjct: 301 IEDYGF-EEGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSISGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of CsaV3_3G028370 vs. NCBI nr
Match:
XP_022936254.1 (uncharacterized protein LOC111442928 [Cucurbita moschata])
HSP 1 Score: 751.9 bits (1940), Expect = 2.8e-213
Identity = 349/394 (88.58%), Postives = 371/394 (94.16%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARR RP+WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+TE+ENCEANV
Sbjct: 61 VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESI+NKS Q HD PFTEDLPRL+CPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY QEDLL+EEIE++Q+KDLTWAVIRPN+IFGFSPF MN++GTLC YAAIC+HEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKTQRKDLTWAVIRPNVIFGFSPFGWMNIIGTLCAYAAICRHEGRPLK 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG K AWE+F VASDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IE+YGF EEGE LTLV+LMKDKS VW+EIVKENQLQ T+LEEIG WWFVD I S+SGNID
Sbjct: 301 IEDYGF-EEGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSISGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of CsaV3_3G028370 vs. ExPASy Swiss-Prot
Match:
Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)
HSP 1 Score: 647.1 bits (1668), Expect = 1.3e-184
Identity = 298/394 (75.63%), Postives = 338/394 (85.79%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRP WNADHPI+YIQCDVSD +D +KLS L DVTH+FYVTWTNR +E ENCEAN
Sbjct: 61 VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
MLRNVL+++IP APNLRH+CLQTGTKHY+G F N RHDPPFTED+PRL+ NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPF---TNVDGPRHDPPFTEDMPRLQIQNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY QED+L+EEI++ + +TW++ RPN+IFGFSP+SLMN+VGTLCVYAAICKHEG PL
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG+K AWE F ASDADLIAEQ IW AVDPY+KNEAFNCNN D+FKWKH WK+LAEQF
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IEEYGF EEG++L LV++MK K VW+E+VKENQLQ+ KLEE+G WWF D I + G ID
Sbjct: 301 IEEYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 SMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388
BLAST of CsaV3_3G028370 vs. ExPASy Swiss-Prot
Match:
A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)
HSP 1 Score: 606.3 bits (1562), Expect = 2.5e-172
Identity = 281/394 (71.32%), Postives = 325/394 (82.49%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKK +EDE PR++ESVAL++GVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRP+WN DHPI YI CDV D D E KLS L DVTH+FY TWT R+TE ENCEAN
Sbjct: 61 VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
KML+NVL ++IPN PNL+HICLQTG KHY+G+FE + S+ HDPP TEDLPRL+ N+
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFEDL---KSKCHDPPLTEDLPRLDSQNY 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY QED+L+EE++ +K+ LTW+V RP IFGFSP+S+MN+VGTLCVYAAICKHEG L+
Sbjct: 181 YYTQEDILFEEVQ--KKEGLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLR 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG K AW+ + SDADLIAE IW AVDPY+KNEAFN +NGDVFKWKHFWKVLAEQF
Sbjct: 241 FPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
+ E G EEG+ + L DLMKDK VWD+IV+EN L TKLE++G WWF D + +D
Sbjct: 301 V-ECGEYEEGQQVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLD 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
+MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 TMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388
BLAST of CsaV3_3G028370 vs. ExPASy Swiss-Prot
Match:
A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)
HSP 1 Score: 601.7 bits (1550), Expect = 6.1e-171
Identity = 280/394 (71.07%), Postives = 324/394 (82.23%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKK+ +EDE PR++ESVAL++GVTGIVGNSLAEILPLSDTP GPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKRIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRP+WN DHPI YI CDV D D E KLS L DVTH+FY TWT R+TE ENCEAN
Sbjct: 61 VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
KML+NVL ++IPN PNL+HICLQTG KHY+G+FE + +RHDPP TEDLPRL+ NF
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFE---DWKIKRHDPPLTEDLPRLDSQNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY QED+L+EE++ +K+ LTW+V RP IFGFSP+S+MN+VGTLCVYAAICKHEG L+
Sbjct: 181 YYTQEDILFEEVQ--KKESLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLR 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG K AW+ + SDADLIAE IW AVDPY+KNEAFN +NGDVFKWKHFWKVLAEQF
Sbjct: 241 FPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
+ E G EEG+ + L DLMKDK VWD+IV+EN L TKLE++G WWF D + +D
Sbjct: 301 V-ECGEYEEGKEVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLD 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
+MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 TMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388
BLAST of CsaV3_3G028370 vs. ExPASy Swiss-Prot
Match:
A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)
HSP 1 Score: 589.3 bits (1518), Expect = 3.1e-167
Identity = 276/395 (69.87%), Postives = 315/395 (79.75%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAA+KKFEED+ PR +SVAL+IGVTGIVGNSLAEILP+SDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAARKKFEEDDAPRDCQSVALIIGVTGIVGNSLAEILPISDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRPAWNADHP+EYIQCD+SD D TKLS L DVTH+F+VTW NR TE E CE N
Sbjct: 61 VARRPRPAWNADHPVEYIQCDISDASDTHTKLSPLTDVTHIFWVTWANRPTESECCELNG 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
MLRNVL ++IP A NL HICLQTG KHY+G FE+ + H+PPFTED+PRL PNF
Sbjct: 121 TMLRNVLNALIPKAANLHHICLQTGHKHYIGPFEAF--GKIKPHEPPFTEDMPRLNAPNF 180
Query: 181 YYKQEDLLWEEIEQSQKK-DLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPL 240
YY ED+L +E S+KK L W+V RP +IFGFSPFS+MN++GTLCVYAAICKHE PL
Sbjct: 181 YYTLEDML---VEASEKKAGLNWSVHRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPL 240
Query: 241 KFPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQF 300
KFPG K AW + VASDADLIAE IW AVDPY+KNEAFNC+NGD+FKWKH WKVLAEQF
Sbjct: 241 KFPGTKAAWNCYSVASDADLIAEHQIWAAVDPYAKNEAFNCSNGDLFKWKHLWKVLAEQF 300
Query: 301 NIEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNI 360
+E FDE + +LV+ MKDK VW+EIV+EN L TKLE + TWWF D I +
Sbjct: 301 GVEYAEFDESEKPTSLVERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVILGGECLL 360
Query: 361 DSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
DSMNKSKEHG+LGFRN+KNS IS IDK+KA KIVP
Sbjct: 361 DSMNKSKEHGYLGFRNTKNSLISVIDKMKAHKIVP 390
BLAST of CsaV3_3G028370 vs. ExPASy Swiss-Prot
Match:
Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)
HSP 1 Score: 579.3 bits (1492), Expect = 3.2e-164
Identity = 272/394 (69.04%), Postives = 316/394 (80.20%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKK+ EED+ SVAL++GVTGI+GNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARR RPAW+ D+PI Y+QCD+SDP D++ KLS L DVTH+FYVTW NR+TE ENCEAN
Sbjct: 61 VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
KM RNVL +VIPN PNL+HI LQTG KHY+G FES + HDPP+TEDLPRL+ NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESY--GKIESHDPPYTEDLPRLKYMNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY ED++ EE+E +K+ LTW+V RP IFGFSP+S+MN+VGTLCVYAAICKHEG+ L+
Sbjct: 181 YYDLEDIMLEEVE--KKEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLR 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
F G K AW+ + SDADLIAE HIW AVDPY+KNEAFN +NGDVFKWKHFWKVLAEQF
Sbjct: 241 FTGCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
+ G EEG L L DLMK K VW+EIV+EN L TKL+++G WWF D I +D
Sbjct: 301 V-GCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILGNECFLD 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKN+FISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389
BLAST of CsaV3_3G028370 vs. ExPASy TrEMBL
Match:
A0A0A0L8Y0 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 PE=3 SV=1)
HSP 1 Score: 834.7 bits (2155), Expect = 1.6e-238
Identity = 394/394 (100.00%), Postives = 394/394 (100.00%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV
Sbjct: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
BLAST of CsaV3_3G028370 vs. ExPASy TrEMBL
Match:
A0A5A7T3B3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001350 PE=3 SV=1)
HSP 1 Score: 798.5 bits (2061), Expect = 1.3e-227
Identity = 375/394 (95.18%), Postives = 385/394 (97.72%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRP WNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRT EI+NCEANV
Sbjct: 61 VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
+MLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFES+INKSSQRH+PPFTEDLPRLECPNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YYKQEDLL+EEIEQSQKKDLTWAVIRPN+IFGFSPFSLMN+VGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG KLAWEDFQ ASDADL+AEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IEEYGF EEGE LTLVDLMKDKSDVWDEIVKENQLQ TKLEEIG WW+VD IFS+SGNID
Sbjct: 301 IEEYGF-EEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of CsaV3_3G028370 vs. ExPASy TrEMBL
Match:
A0A1S3BHA7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC103489839 PE=3 SV=1)
HSP 1 Score: 798.5 bits (2061), Expect = 1.3e-227
Identity = 375/394 (95.18%), Postives = 385/394 (97.72%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRP WNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRT EI+NCEANV
Sbjct: 61 VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
+MLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFES+INKSSQRH+PPFTEDLPRLECPNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YYKQEDLL+EEIEQSQKKDLTWAVIRPN+IFGFSPFSLMN+VGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG KLAWEDFQ ASDADL+AEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IEEYGF EEGE LTLVDLMKDKSDVWDEIVKENQLQ TKLEEIG WW+VD IFS+SGNID
Sbjct: 301 IEEYGF-EEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of CsaV3_3G028370 vs. ExPASy TrEMBL
Match:
A0A6J1F7T0 (uncharacterized protein LOC111442928 OS=Cucurbita moschata OX=3662 GN=LOC111442928 PE=3 SV=1)
HSP 1 Score: 751.9 bits (1940), Expect = 1.3e-213
Identity = 349/394 (88.58%), Postives = 371/394 (94.16%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARR RP+WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+TE+ENCEANV
Sbjct: 61 VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESI+NKS Q HD PFTEDLPRL+CPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY QEDLL+EEIE++Q+KDLTWAVIRPN+IFGFSPF MN++GTLC YAAIC+HEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKTQRKDLTWAVIRPNVIFGFSPFGWMNIIGTLCAYAAICRHEGRPLK 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG K AWE+F VASDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IE+YGF EEGE LTLV+LMKDKS VW+EIVKENQLQ T+LEEIG WWFVD I S+SGNID
Sbjct: 301 IEDYGF-EEGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSISGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of CsaV3_3G028370 vs. ExPASy TrEMBL
Match:
A0A6J1IQC1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111477228 PE=3 SV=1)
HSP 1 Score: 742.3 bits (1915), Expect = 1.1e-210
Identity = 346/394 (87.82%), Postives = 368/394 (93.40%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKK F+EDEPPR FE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKTFDEDEPPRRFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARR RP+WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+TE+ENCEANV
Sbjct: 61 VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
KMLRNVLRSVI NAPNLRHICLQTGTKHYVGSFESI+NKS Q PFTEDLPRL+CPNF
Sbjct: 121 KMLRNVLRSVISNAPNLRHICLQTGTKHYVGSFESIVNKSVQPPGSPFTEDLPRLKCPNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY QEDLL+EEIE++Q+KDLTWAVIRPN+IFGFSPFS MN++GTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKTQRKDLTWAVIRPNVIFGFSPFSWMNIIGTLCAYAAICKHEGRPLK 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG K AWE+F VASDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IE+YGF EEGE LTLV+LMKDKS VW+EIVKENQLQ T+LEEIG WWFVD I S+SGNID
Sbjct: 301 IEDYGF-EEGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSISGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of CsaV3_3G028370 vs. TAIR 10
Match:
AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 647.1 bits (1668), Expect = 9.0e-186
Identity = 298/394 (75.63%), Postives = 338/394 (85.79%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRP WNADHPI+YIQCDVSD +D +KLS L DVTH+FYVTWTNR +E ENCEAN
Sbjct: 61 VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
MLRNVL+++IP APNLRH+CLQTGTKHY+G F N RHDPPFTED+PRL+ NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPF---TNVDGPRHDPPFTEDMPRLQIQNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY QED+L+EEI++ + +TW++ RPN+IFGFSP+SLMN+VGTLCVYAAICKHEG PL
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG+K AWE F ASDADLIAEQ IW AVDPY+KNEAFNCNN D+FKWKH WK+LAEQF
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IEEYGF EEG++L LV++MK K VW+E+VKENQLQ+ KLEE+G WWF D I + G ID
Sbjct: 301 IEEYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 SMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388
BLAST of CsaV3_3G028370 vs. TAIR 10
Match:
AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 640.2 bits (1650), Expect = 1.1e-183
Identity = 297/394 (75.38%), Postives = 337/394 (85.53%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
MSWWWAGAIGAA KK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAA-KKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
VARRPRP WNADHPI+YIQCDVSD +D +KLS L DVTH+FYVTWTNR +E ENCEAN
Sbjct: 61 VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120
Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
MLRNVL+++IP APNLRH+CLQTGTKHY+G F N RHDPPFTED+PRL+ NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPF---TNVDGPRHDPPFTEDMPRLQIQNF 180
Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
YY QED+L+EEI++ + +TW++ RPN+IFGFSP+SLMN+VGTLCVYAAICKHEG PL
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240
Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG+K AWE F ASDADLIAEQ IW AVDPY+KNEAFNCNN D+FKWKH WK+LAEQF
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300
Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
IEEYGF EEG++L LV++MK K VW+E+VKENQLQ+ KLEE+G WWF D I + G ID
Sbjct: 301 IEEYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
SMNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 SMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 387
BLAST of CsaV3_3G028370 vs. TAIR 10
Match:
AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 233.4 bits (594), Expect = 3.1e-61
Identity = 135/377 (35.81%), Postives = 205/377 (54.38%), Query Frame = 0
Query: 26 ESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPAWNADHPIEYIQCDVSDP 85
E+VAL+ GVTG+VG + + L L PG W++YGVAR P + +I CD+ +
Sbjct: 16 ENVALIFGVTGLVGREIVKTL-LMSKPG--WRIYGVARNPE-INSMTKMYNFISCDLLNA 75
Query: 86 QDAETKLSQLAD-VTHLFYVTWTNR--TTEIENCEANVKMLRNVLRSVIPNAPNLRHICL 145
+ + +LS L D V+H+F+VTW+ E C N ML N L +++PNA L+H L
Sbjct: 76 SETKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSL 135
Query: 146 QTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLEC-PNFYYKQEDLLWEEIEQSQKKDLT 205
QTG KHYV E + + ++E+ PR NFYY EDLL E+I +S +
Sbjct: 136 QTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRS---SVV 195
Query: 206 WAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLKFPGNKLAWEDFQV-ASDADLI 265
W+V RP L+ G S +L N +G+LCVY A+CK+ P F G + WE+ + SD++L+
Sbjct: 196 WSVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLV 255
Query: 266 AEQHIWTAVD--PYSKNEAFNCNNGDVFKWKHFWKVLAEQFNIEEYGFDEEGESLTLVDL 325
AEQHI+ A K EAFN NG F WK W + ++ ++ E
Sbjct: 256 AEQHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGFWFGRE 315
Query: 326 MKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGN-IDSMNKSKEHGFLGFRNSK 385
M ++ VWDEIV + +L +T++E++ W+F+D++F + K GF +
Sbjct: 316 MVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTL 375
Query: 386 NSFISWIDKIKAFKIVP 395
+S + WID ++ K++P
Sbjct: 376 DSVLYWIDVMRDEKLIP 385
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_004152001.1 | 3.3e-238 | 100.00 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN58303.1 hypothet... | [more] |
XP_008447374.1 | 2.6e-227 | 95.18 | PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo] >KAA0037954.... | [more] |
XP_038899926.1 | 9.3e-217 | 90.61 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Benincasa hispida] | [more] |
XP_023535925.1 | 1.9e-214 | 88.83 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo] | [more] |
XP_022936254.1 | 2.8e-213 | 88.58 | uncharacterized protein LOC111442928 [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
Q9STX2 | 1.3e-184 | 75.63 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... | [more] |
A0A221J5W8 | 2.5e-172 | 71.32 | (S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ... | [more] |
A0A221J5X3 | 6.1e-171 | 71.07 | (S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1... | [more] |
A0A1C9CX66 | 3.1e-167 | 69.87 | (S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC... | [more] |
Q6PQJ9 | 3.2e-164 | 69.04 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0L8Y0 | 1.6e-238 | 100.00 | Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 P... | [more] |
A0A5A7T3B3 | 1.3e-227 | 95.18 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695... | [more] |
A0A1S3BHA7 | 1.3e-227 | 95.18 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC10348983... | [more] |
A0A6J1F7T0 | 1.3e-213 | 88.58 | uncharacterized protein LOC111442928 OS=Cucurbita moschata OX=3662 GN=LOC1114429... | [more] |
A0A6J1IQC1 | 1.1e-210 | 87.82 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO... | [more] |
Match Name | E-value | Identity | Description | |
AT4G24220.1 | 9.0e-186 | 75.63 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |
AT4G24220.2 | 1.1e-183 | 75.38 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |
AT5G58750.1 | 3.1e-61 | 35.81 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |