Homology
BLAST of CsaV3_3G022960 vs. NCBI nr
Match:
XP_004152028.1 (taxadiene 5-alpha hydroxylase [Cucumis sativus] >KGN57973.1 hypothetical protein Csa_010468 [Cucumis sativus])
HSP 1 Score: 962.2 bits (2486), Expect = 1.7e-276
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0
Query: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ
Sbjct: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
Query: 61 KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL
Sbjct: 61 KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
Query: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK
Sbjct: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
Query: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT
Sbjct: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360
LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG
Sbjct: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360
Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI
Sbjct: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK
Sbjct: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
Query: 481 S 482
S
Sbjct: 481 S 481
BLAST of CsaV3_3G022960 vs. NCBI nr
Match:
XP_008454553.1 (PREDICTED: LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like [Cucumis melo])
HSP 1 Score: 901.7 bits (2329), Expect = 2.7e-258
Identity = 449/481 (93.35%), Postives = 466/481 (96.88%), Query Frame = 0
Query: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
MIT IILLV+TIFLFHRFRTR SSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ
Sbjct: 1 MITPIILLVITIFLFHRFRTRFSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
Query: 61 KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
+RVDKYGPVSKMTLFGKPTVF+HGAAANKAVVFSGEEGTVSNRQ+ESLKMILGKRNLTEL
Sbjct: 61 QRVDKYGPVSKMTLFGKPTVFVHGAAANKAVVFSGEEGTVSNRQIESLKMILGKRNLTEL 120
Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
SGEDHKRVRGALVWFLRPQTLRSYV KMDGEVRRHLNMYWHGNN+VTVAPLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQTLRSYVEKMDGEVRRHLNMYWHGNNKVTVAPLMKTLTFDII 180
Query: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
CSLLFGIEEGTTRKSII+CFKTMV+GIWS+PINLPFTRYNHSLKASAK QQILKQLLKDK
Sbjct: 181 CSLLFGIEEGTTRKSIIQCFKTMVEGIWSVPINLPFTRYNHSLKASAKVQQILKQLLKDK 240
Query: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
AK ME++K ++++ KDLI+YLLRIKNKDKEQAL+EEEI+HNIILLMIAGHDTTSILLT
Sbjct: 241 AKRMEQKK--KMKKKMKDLITYLLRIKNKDKEQALTEEEILHNIILLMIAGHDTTSILLT 300
Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360
LMLRVLATNPTVYA VLQEHEEI RSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG
Sbjct: 301 LMLRVLATNPTVYADVLQEHEEIARSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360
Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
GFRVALKDIQLGAYTIPKGWQIFWAA THLDETIFGDPQKFEPSRFDQ QTPIPPFSFI
Sbjct: 361 GFRVALKDIQLGAYTIPKGWQIFWAASTTHLDETIFGDPQKFEPSRFDQTQTPIPPFSFI 420
Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQD LTRDPTLMPNKGLPIQIFPK
Sbjct: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDSLTRDPTLMPNKGLPIQIFPK 479
Query: 481 S 482
S
Sbjct: 481 S 479
BLAST of CsaV3_3G022960 vs. NCBI nr
Match:
XP_038899676.1 (taxadiene 5-alpha hydroxylase-like [Benincasa hispida])
HSP 1 Score: 866.7 bits (2238), Expect = 9.5e-248
Identity = 435/481 (90.44%), Postives = 455/481 (94.59%), Query Frame = 0
Query: 1 MITVIILLVLTIFLFHRFRTRLSSSA-AKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWL 60
MIT+ +LL+ TIFLF RFRT SS+A AKNLPPG LGVPFIGQSLSLL AMRTN+AE+WL
Sbjct: 1 MITIFLLLI-TIFLFLRFRTTSSSAATAKNLPPGPLGVPFIGQSLSLLRAMRTNSAEQWL 60
Query: 61 QKRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTE 120
QKRV KYGPVSKMTLFGKPTVF+HGAAANKAVVF GEEGTVSNRQ+ESLK ILG+RNLTE
Sbjct: 61 QKRVAKYGPVSKMTLFGKPTVFVHGAAANKAVVFLGEEGTVSNRQIESLKWILGERNLTE 120
Query: 121 LSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDI 180
LSGEDHKRVRGALVWFLRPQTL+SYVGKMDGEVR+HLNMYWHG EVTVAPLMKTLTFDI
Sbjct: 121 LSGEDHKRVRGALVWFLRPQTLKSYVGKMDGEVRKHLNMYWHGKKEVTVAPLMKTLTFDI 180
Query: 181 ICSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKD 240
ICSLLFGIEEG TRKSII+CFKTMVDGIWS+PINLPFTRYNHSLKASAKAQQILKQLLKD
Sbjct: 181 ICSLLFGIEEGPTRKSIIQCFKTMVDGIWSVPINLPFTRYNHSLKASAKAQQILKQLLKD 240
Query: 241 KAKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILL 300
KA+ MEEE+E E +E+DKDLI+YLL IKNKDKEQALSEEEIVHN ILLMIAGHDTTSILL
Sbjct: 241 KAQKMEEEEEEE-KEDDKDLITYLLSIKNKDKEQALSEEEIVHNTILLMIAGHDTTSILL 300
Query: 301 TLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVF 360
TLMLRVLATNPTVYAAVLQEHEEI RSKE GEALTWEDVSKMKYTWRVAMETLR+YPPVF
Sbjct: 301 TLMLRVLATNPTVYAAVLQEHEEIARSKESGEALTWEDVSKMKYTWRVAMETLRIYPPVF 360
Query: 361 GGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSF 420
GGFRVALKDIQLG YTIPKGWQIFWAAPMTHLDETIFG+PQKFEPSRFDQNQTPIPPF F
Sbjct: 361 GGFRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGEPQKFEPSRFDQNQTPIPPFCF 420
Query: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFP 480
IAFGGGPRICPG EFAKLETLVTIHYLITQFTW LSCSQDFLTRDPTLMPNKGLPIQI P
Sbjct: 421 IAFGGGPRICPGSEFAKLETLVTIHYLITQFTWKLSCSQDFLTRDPTLMPNKGLPIQISP 479
BLAST of CsaV3_3G022960 vs. NCBI nr
Match:
XP_023535435.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 807.4 bits (2084), Expect = 6.8e-230
Identity = 403/477 (84.49%), Postives = 439/477 (92.03%), Query Frame = 0
Query: 5 IILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVD 64
IILL++TIFL+ R RT S+AAKNLPPG LGVP+IGQSLSLL AMR N+AE+WLQ+RV
Sbjct: 4 IILLLITIFLYLRLRTTFFSAAAKNLPPGRLGVPYIGQSLSLLRAMRANSAEQWLQQRVA 63
Query: 65 KYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGED 124
KYGP+SKMTLFGKPTVF+HG AANKAVVF GEE VSNRQ ESL+ ILG+RNLTELSGED
Sbjct: 64 KYGPISKMTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGED 123
Query: 125 HKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLL 184
HKRVRGALVWFLRPQ+L+ +VGKMDGEVR+HLN++WHGN EV VAPLMKTLTFDIICSLL
Sbjct: 124 HKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLL 183
Query: 185 FGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKAKVM 244
FGIEEG TRKSIIECFKTMVDGIWS+PINLPFTRY+HSLKASAK QQILKQLLKDKA+ M
Sbjct: 184 FGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKM 243
Query: 245 EEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLR 304
EEE+E +E DK+LI+ LL IKNKDKEQALSEEEIVHNIILL+IAGHDTTSIL+TLMLR
Sbjct: 244 EEEEE---KEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLR 303
Query: 305 VLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRV 364
V+ATNPTVYAAVLQEHE+I RSKE GEALTWEDVSKMKYTWRVA+ETLRL+PPVFGGFR+
Sbjct: 304 VMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRM 363
Query: 365 ALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPI-PPFSFIAFG 424
ALKDIQLG YTIPKGWQIFWAA MTHLDE IF DPQKFEPSRFDQNQT I PP SFIAFG
Sbjct: 364 ALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFG 423
Query: 425 GGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 481
GGPRICPGYEFAKLETLVTIHY+ITQFTW LS SQDFLTRDP+LMPNKGLPIQ++PK
Sbjct: 424 GGPRICPGYEFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 476
BLAST of CsaV3_3G022960 vs. NCBI nr
Match:
XP_022976494.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita maxima])
HSP 1 Score: 803.9 bits (2075), Expect = 7.5e-229
Identity = 403/477 (84.49%), Postives = 438/477 (91.82%), Query Frame = 0
Query: 6 ILLVLTIFLFHRFR-TRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVD 65
ILL++TIFL+ R R T SS+ AKNLPPG LGVPFIGQSLSLL AMR N+AE+WLQ+RV
Sbjct: 5 ILLLITIFLYLRLRATFFSSAEAKNLPPGPLGVPFIGQSLSLLRAMRANSAEQWLQQRVA 64
Query: 66 KYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGED 125
KYGP+SKMTLFGKPTVF+HG AANKAVVFSGEE VSNRQ ESL+ ILG+RNLTELSGED
Sbjct: 65 KYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERNLTELSGED 124
Query: 126 HKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLL 185
HKRVRGALVWFLRPQ+L+ +VGKMDGEVR+HLN++WHGN EV VAPLMKTLTFDIICSLL
Sbjct: 125 HKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLL 184
Query: 186 FGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKAKVM 245
FGIEEG TRKSIIECFKTMVDGIWS+PINLPFTRY+HSL ASAK QQILKQLLKDKA+ M
Sbjct: 185 FGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLNASAKVQQILKQLLKDKAQKM 244
Query: 246 EEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLR 305
EEE+E +E DK+LI+ LL IKNKDKEQALSEEEIVHNIILL+IAGHDTTSIL+TLMLR
Sbjct: 245 EEEEEE--KEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLR 304
Query: 306 VLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRV 365
V+ATNPTVYAAVLQEHE+I RSKE GEALTWEDVSKMKYTWRVA+ETLRL+PPVFGGFRV
Sbjct: 305 VMATNPTVYAAVLQEHEDIHRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRV 364
Query: 366 ALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPI-PPFSFIAFG 425
ALKDIQLG YTIPKGWQIFWAA MTHLDE IF DPQKF+PSRFDQNQT I PP SFIAFG
Sbjct: 365 ALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFTDPQKFKPSRFDQNQTSIPPPLSFIAFG 424
Query: 426 GGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 481
GGPRICPGYEFAKLETLVTIHY+ITQFTW LS SQDFLTRDP+LMPNKGLPIQ++PK
Sbjct: 425 GGPRICPGYEFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 478
BLAST of CsaV3_3G022960 vs. ExPASy Swiss-Prot
Match:
Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)
HSP 1 Score: 348.6 bits (893), Expect = 1.1e-94
Identity = 186/477 (38.99%), Postives = 291/477 (61.01%), Query Frame = 0
Query: 6 ILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVDK 65
ILL+L +F R ++ LPPG LG+PFIG+S L A+R+N+ E++ +RV K
Sbjct: 35 ILLLLLLF-------RSKRHSSLKLPPGKLGIPFIGESFIFLRALRSNSLEQFFDERVKK 94
Query: 66 YGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGEDH 125
+G V K +L G PTV + G A N+ ++ S EE V ++G+ ++ GEDH
Sbjct: 95 FGLVFKTSLIGHPTVVLCGPAGNR-LILSNEEKLVQMSWPAQFMKLMGENSVATRRGEDH 154
Query: 126 KRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLLF 185
+R AL F P L+SY+GKM+ E++ H+N W G +EV V PL++ L F+I L F
Sbjct: 155 IVMRSALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAILFF 214
Query: 186 GIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKAKVME 245
I + + + + +T++ G +++PI+LP ++ +L+ AK +I+ L+K + +
Sbjct: 215 NIYDKQEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKR----K 274
Query: 246 EEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLRV 305
E+ +S +DL+S LL ++ DK L+ +EI+ N L+ A +DTT+ + L+ ++
Sbjct: 275 EDLQSGSATATQDLLSVLLTFRD-DKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKL 334
Query: 306 LATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRVA 365
L++NP Y V+QE EI +KE GE +TW+D+ MKYTW+VA ETLR++PPVFG FR A
Sbjct: 335 LSSNPECYQKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKA 394
Query: 366 LKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFIAFGGG 425
+ DIQ YTIPKGW++ W TH + F +P+KF PSRFDQ + P++F+ FGGG
Sbjct: 395 ITDIQYDGYTIPKGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHVAPYTFLPFGGG 454
Query: 426 PRICPGYEFAKLETLVTIHYLITQFTWNLSCSQD-FLTRDP-TLMPNKGLPIQIFPK 481
R C G+EF+K+E L+ +H+ + F+ D ++ DP +P+KG I++FP+
Sbjct: 455 QRSCVGWEFSKMEILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498
BLAST of CsaV3_3G022960 vs. ExPASy Swiss-Prot
Match:
Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)
HSP 1 Score: 341.3 bits (874), Expect = 1.8e-92
Identity = 180/483 (37.27%), Postives = 287/483 (59.42%), Query Frame = 0
Query: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
+++ + +VL + LF R + R ++ LPPG LG PFIG+SL L A+R+NT E++L
Sbjct: 25 VLSAVAGIVLPLLLFLRSKRR----SSVGLPPGKLGYPFIGESLLFLKALRSNTVEQFLD 84
Query: 61 KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
+RV +G V K +L G PTV + G A N+ ++ + EE V +S ++G++++T
Sbjct: 85 ERVKNFGNVFKTSLIGHPTVVLCGPAGNR-LILANEEKLVQMSWPKSSMKLMGEKSITAK 144
Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
GE H +R AL F P L+ Y+G+M + H+N W GN++V+V L+ L FDI
Sbjct: 145 RGEGHMIIRSALQGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVALVGDLVFDIS 204
Query: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
L F I E R+ + E + + G+ ++P++LP Y+ +L+A +K IL L++ +
Sbjct: 205 ACLFFNINEKHERERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLNAILSGLIEKR 264
Query: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
+ + S + +++DL+S L K+ D+ S+EEI+ N L+ +DTT +
Sbjct: 265 ----KMDLSSGLATSNQDLLSVFLTFKD-DRGNPCSDEEILDNFSGLLHGSYDTTVSAMA 324
Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360
+ ++L++NP Y V+QE I +K G+ +TW+DV MKYTW+V ETLRLYP +FG
Sbjct: 325 CVFKLLSSNPECYEKVVQEQLGILSNKLEGDEITWKDVKSMKYTWQVVQETLRLYPSIFG 384
Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
FR A+ DI Y IPKGW++ W TH E F +P+KF PSRFDQ + P++F+
Sbjct: 385 SFRQAITDIHYNGYIIPKGWKLLWTPYTTHPKEMYFSEPEKFLPSRFDQEGKLVAPYTFL 444
Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQF-TWNLSCSQDFLTRDPTL-MPNKGLPIQIF 480
FGGG R CPG+EF+K+E L+++H+ + F T+ + + RD +P+ G +++F
Sbjct: 445 PFGGGQRSCPGWEFSKMEILLSVHHFVKTFSTFTPVDPAEIIARDSLCPLPSNGFSVKLF 497
Query: 481 PKS 482
P+S
Sbjct: 505 PRS 497
BLAST of CsaV3_3G022960 vs. ExPASy Swiss-Prot
Match:
Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)
HSP 1 Score: 339.7 bits (870), Expect = 5.3e-92
Identity = 187/480 (38.96%), Postives = 273/480 (56.88%), Query Frame = 0
Query: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
M TV + + + F + + A + +PPG G P IG++L LG R +
Sbjct: 22 MFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFD 81
Query: 61 KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
R KYG V +L G PTV N+ +FS E V N S+ + + +L
Sbjct: 82 SRTQKYGNVFTTSLVGHPTVVFCSPEGNR-FLFSNENKLVVNSWPSSVGNLF-RSSLITT 141
Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
G+D KR+R L+ FLRP+ LR +VG++D +RHL +W G +EV PL+K TF +
Sbjct: 142 VGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLA 201
Query: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
C L I + F V G+ IPI+LP TRYN + A+ +Q L ++ ++
Sbjct: 202 CDLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINER 261
Query: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKE-QALSEEEIVHNIILLMIAGHDTTSILL 300
+E S ++DL+S+LL N D++ ++L++ EI NI+LL+ AGHDT+S L
Sbjct: 262 KIALEAGNASP----EQDLLSFLL--SNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTL 321
Query: 301 TLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVF 360
T++L+ LA NP Y VL+E I SKE G+ L WED+ +MKY+WRVA E LRL+P V
Sbjct: 322 TVLLKFLAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQ 381
Query: 361 GGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSF 420
G FR A+K+ +TIPKGW++ W TH F +P+KF+PSRF + + P PP++F
Sbjct: 382 GSFRKAIKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRF-EGEGP-PPYTF 441
Query: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFP 480
+ FGGGPR+CPG EFA++E L+ +H ++ F WNL + + DP P GLPI++ P
Sbjct: 442 VPFGGGPRMCPGNEFARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491
BLAST of CsaV3_3G022960 vs. ExPASy Swiss-Prot
Match:
Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)
HSP 1 Score: 336.3 bits (861), Expect = 5.8e-91
Identity = 187/480 (38.96%), Postives = 273/480 (56.88%), Query Frame = 0
Query: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
M TV + + + F + + A + +PPG G P IG++L LG R +
Sbjct: 22 MFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFD 81
Query: 61 KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
R KYG V +L G PTV N+ +FS E V N S+ + + +L
Sbjct: 82 SRTQKYGNVFTTSLVGHPTVVFCSPEGNR-FLFSNENKLVVNSWPSSVGNLF-RSSLITT 141
Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
G+D KR+R L+ FLRP+ LR +VG++D +RHL +W G +EVT PL+K TF +
Sbjct: 142 VGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLA 201
Query: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
C L I + F V G+ IPI+LP TRYN + A+ +Q L ++ ++
Sbjct: 202 CDLFASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINER 261
Query: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKE-QALSEEEIVHNIILLMIAGHDTTSILL 300
+E S ++DL+S+LL N D++ ++L++ EI NI+LL+ AGHDT+S L
Sbjct: 262 KIGLEAGNASP----EQDLLSFLL--SNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTL 321
Query: 301 TLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVF 360
T++L+ LA NP Y VL+E +I SKE G+ L WED+ +MKY+WRVA E LRL+P
Sbjct: 322 TVLLKFLAENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQ 381
Query: 361 GGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSF 420
G FR A+K+ +TIPKGW+++W TH F +P+ F+PSRF + + P PP++F
Sbjct: 382 GSFRKAIKEFIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRF-EGEGP-PPYTF 441
Query: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFP 480
+ FGGGPR+CPG EFA+LE LV +H ++ WNL + + DP P GLPI++ P
Sbjct: 442 VPFGGGPRMCPGNEFARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVP 491
BLAST of CsaV3_3G022960 vs. ExPASy Swiss-Prot
Match:
Q9AXM6 (Taxane 10-beta-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A1 PE=1 SV=1)
HSP 1 Score: 336.3 bits (861), Expect = 5.8e-91
Identity = 174/482 (36.10%), Postives = 286/482 (59.34%), Query Frame = 0
Query: 2 ITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQK 61
+T+ +L + + L R+ R ++ LPPG LG P IG+++ LL +R+ T +K+
Sbjct: 26 LTLAPILAIILLLLFRYNHR----SSVKLPPGKLGFPLIGETIQLLRTLRSETPQKFFDD 85
Query: 62 RVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELS 121
R+ K+GPV +L G PTV + G A NK +V S E+ V +S ++G+ ++
Sbjct: 86 RLKKFGPVYMTSLIGHPTVVLCGPAGNK-LVLSNEDKLVEMEGPKSFMKLIGEDSIVAKR 145
Query: 122 GEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIIC 181
GEDH+ +R AL FL Q L++Y+G+M E+ H N W G +EV V PL++ L F I
Sbjct: 146 GEDHRILRTALARFLGAQALQNYLGRMSSEIGHHFNEKWKGKDEVKVLPLVRGLIFSIAS 205
Query: 182 SLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKA 241
+L F + +G +K + +T++ G S+P++ P TRY L+A K +IL L+K +
Sbjct: 206 TLFFDVNDGHQQKQLHHLLETILVGSLSVPLDFPGTRYRKGLQARLKLDEILSSLIKRRR 265
Query: 242 KVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTL 301
+ + S + +D+DL+S LL ++ +K +L+++ I+ N + A +DTT + L
Sbjct: 266 R----DLRSGIASDDQDLLSVLLTFRD-EKGNSLTDQGILDNFSAMFHASYDTTVAPMAL 325
Query: 302 MLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGG 361
+ ++L +NP + V QE EI +K+ GE ++W+D+ MKYTW+ E+LR+YPPVFG
Sbjct: 326 IFKLLYSNPEYHEKVFQEQLEIIGNKKEGEEISWKDLKSMKYTWQAVQESLRMYPPVFGI 385
Query: 362 FRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFIA 421
FR A+ DI YTIPKGW++ + THL E F +P++F PSRF+ + P++++
Sbjct: 386 FRKAITDIHYDGYTIPKGWRVLCSPYTTHLREEYFPEPEEFRPSRFEDEGRHVTPYTYVP 445
Query: 422 FGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSC--SQDFLTRDPTLMPNKGLPIQIFP 481
FGGG R CPG+EF+K+E L+ +H+ + F+ + ++ L+ +P G I++FP
Sbjct: 446 FGGGLRTCPGWEFSKIEILLFVHHFVKNFSSYIPVDPNEKVLSDPLPPLPANGFSIKLFP 497
BLAST of CsaV3_3G022960 vs. ExPASy TrEMBL
Match:
A0A0A0L864 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G416660 PE=3 SV=1)
HSP 1 Score: 962.2 bits (2486), Expect = 8.0e-277
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0
Query: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ
Sbjct: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
Query: 61 KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL
Sbjct: 61 KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
Query: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK
Sbjct: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
Query: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT
Sbjct: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360
LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG
Sbjct: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360
Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI
Sbjct: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK
Sbjct: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
Query: 481 S 482
S
Sbjct: 481 S 481
BLAST of CsaV3_3G022960 vs. ExPASy TrEMBL
Match:
A0A1S3C037 (LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103494945 PE=3 SV=1)
HSP 1 Score: 901.7 bits (2329), Expect = 1.3e-258
Identity = 449/481 (93.35%), Postives = 466/481 (96.88%), Query Frame = 0
Query: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
MIT IILLV+TIFLFHRFRTR SSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ
Sbjct: 1 MITPIILLVITIFLFHRFRTRFSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
Query: 61 KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
+RVDKYGPVSKMTLFGKPTVF+HGAAANKAVVFSGEEGTVSNRQ+ESLKMILGKRNLTEL
Sbjct: 61 QRVDKYGPVSKMTLFGKPTVFVHGAAANKAVVFSGEEGTVSNRQIESLKMILGKRNLTEL 120
Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
SGEDHKRVRGALVWFLRPQTLRSYV KMDGEVRRHLNMYWHGNN+VTVAPLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQTLRSYVEKMDGEVRRHLNMYWHGNNKVTVAPLMKTLTFDII 180
Query: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
CSLLFGIEEGTTRKSII+CFKTMV+GIWS+PINLPFTRYNHSLKASAK QQILKQLLKDK
Sbjct: 181 CSLLFGIEEGTTRKSIIQCFKTMVEGIWSVPINLPFTRYNHSLKASAKVQQILKQLLKDK 240
Query: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
AK ME++K ++++ KDLI+YLLRIKNKDKEQAL+EEEI+HNIILLMIAGHDTTSILLT
Sbjct: 241 AKRMEQKK--KMKKKMKDLITYLLRIKNKDKEQALTEEEILHNIILLMIAGHDTTSILLT 300
Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360
LMLRVLATNPTVYA VLQEHEEI RSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG
Sbjct: 301 LMLRVLATNPTVYADVLQEHEEIARSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360
Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
GFRVALKDIQLGAYTIPKGWQIFWAA THLDETIFGDPQKFEPSRFDQ QTPIPPFSFI
Sbjct: 361 GFRVALKDIQLGAYTIPKGWQIFWAASTTHLDETIFGDPQKFEPSRFDQTQTPIPPFSFI 420
Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQD LTRDPTLMPNKGLPIQIFPK
Sbjct: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDSLTRDPTLMPNKGLPIQIFPK 479
Query: 481 S 482
S
Sbjct: 481 S 479
BLAST of CsaV3_3G022960 vs. ExPASy TrEMBL
Match:
A0A6J1INM7 (taxadiene 5-alpha hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111476875 PE=3 SV=1)
HSP 1 Score: 803.9 bits (2075), Expect = 3.7e-229
Identity = 403/477 (84.49%), Postives = 438/477 (91.82%), Query Frame = 0
Query: 6 ILLVLTIFLFHRFR-TRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVD 65
ILL++TIFL+ R R T SS+ AKNLPPG LGVPFIGQSLSLL AMR N+AE+WLQ+RV
Sbjct: 5 ILLLITIFLYLRLRATFFSSAEAKNLPPGPLGVPFIGQSLSLLRAMRANSAEQWLQQRVA 64
Query: 66 KYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGED 125
KYGP+SKMTLFGKPTVF+HG AANKAVVFSGEE VSNRQ ESL+ ILG+RNLTELSGED
Sbjct: 65 KYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERNLTELSGED 124
Query: 126 HKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLL 185
HKRVRGALVWFLRPQ+L+ +VGKMDGEVR+HLN++WHGN EV VAPLMKTLTFDIICSLL
Sbjct: 125 HKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLL 184
Query: 186 FGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKAKVM 245
FGIEEG TRKSIIECFKTMVDGIWS+PINLPFTRY+HSL ASAK QQILKQLLKDKA+ M
Sbjct: 185 FGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLNASAKVQQILKQLLKDKAQKM 244
Query: 246 EEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLR 305
EEE+E +E DK+LI+ LL IKNKDKEQALSEEEIVHNIILL+IAGHDTTSIL+TLMLR
Sbjct: 245 EEEEEE--KEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLR 304
Query: 306 VLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRV 365
V+ATNPTVYAAVLQEHE+I RSKE GEALTWEDVSKMKYTWRVA+ETLRL+PPVFGGFRV
Sbjct: 305 VMATNPTVYAAVLQEHEDIHRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRV 364
Query: 366 ALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPI-PPFSFIAFG 425
ALKDIQLG YTIPKGWQIFWAA MTHLDE IF DPQKF+PSRFDQNQT I PP SFIAFG
Sbjct: 365 ALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFTDPQKFKPSRFDQNQTSIPPPLSFIAFG 424
Query: 426 GGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 481
GGPRICPGYEFAKLETLVTIHY+ITQFTW LS SQDFLTRDP+LMPNKGLPIQ++PK
Sbjct: 425 GGPRICPGYEFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 478
BLAST of CsaV3_3G022960 vs. ExPASy TrEMBL
Match:
A0A6J1F7G6 (taxadiene 5-alpha hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443055 PE=3 SV=1)
HSP 1 Score: 800.4 bits (2066), Expect = 4.0e-228
Identity = 402/479 (83.92%), Postives = 438/479 (91.44%), Query Frame = 0
Query: 5 IILLVLTIFLFHRFRTRLSSS--AAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKR 64
IILL++TIF++ R RT S+ AAKNLPPG LGVP+IGQSLSLL AMR N+AE+WLQ+R
Sbjct: 4 IILLLITIFVYLRLRTTFFSTAVAAKNLPPGRLGVPYIGQSLSLLRAMRANSAEQWLQQR 63
Query: 65 VDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSG 124
V KYGP+SKMTLFGKPTVF+HG AANKAVVFSGEE VSNRQ ESL+ ILG+RNLTELSG
Sbjct: 64 VAKYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERNLTELSG 123
Query: 125 EDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICS 184
EDHKRVRGALVWFLRPQ+L+ +VGKMDGEVR+HL ++WHGN EV VAPLMKTLTFDIICS
Sbjct: 124 EDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLKLHWHGNKEVIVAPLMKTLTFDIICS 183
Query: 185 LLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKAK 244
LLFGIEEG TRKSIIECFKTMVDGIWS+PINLPFTRY+HSLKASAK QQILKQLLKDKA+
Sbjct: 184 LLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQ 243
Query: 245 VMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLM 304
ME E+E +E DK+LI+ LL IKNKDKEQALSEEEIVHNIILL+IAGHDTTSIL+TLM
Sbjct: 244 KMEGEEEE--KEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLM 303
Query: 305 LRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGGF 364
LRV+ATNPTVYAAVLQEHE+I RSKE GEALTWEDVSKMKYTWRVA+ETLRL+PPVFGGF
Sbjct: 304 LRVMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGF 363
Query: 365 RVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPI-PPFSFIA 424
RVALKDIQLG YTIPKGWQIFWAA MTHLDE IF DPQKFEPSRFDQNQT I PP SFIA
Sbjct: 364 RVALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIA 423
Query: 425 FGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 481
FGGGPRICPGYEFAKLETLVTIHY+ITQFTW LS SQDFLTRDP+LMPNKGLPIQ++PK
Sbjct: 424 FGGGPRICPGYEFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 479
BLAST of CsaV3_3G022960 vs. ExPASy TrEMBL
Match:
A0A6J1CCZ3 (taxadiene 5-alpha hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111010356 PE=3 SV=1)
HSP 1 Score: 732.3 bits (1889), Expect = 1.4e-207
Identity = 363/480 (75.62%), Postives = 414/480 (86.25%), Query Frame = 0
Query: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
+I + L +LTIF R SS LPPG LG+P+IGQSL LL AMR+N+AE+WL
Sbjct: 2 IIPMFFLFLLTIFFLFLTTRRTRSS---RLPPGPLGLPYIGQSLGLLRAMRSNSAEQWLL 61
Query: 61 KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
+RV KYGPVSKMTLFGKPTVFIHG ANKA VF+GEEG +SN+Q ESLK ILG+RNLTEL
Sbjct: 62 RRVAKYGPVSKMTLFGKPTVFIHGTVANKA-VFTGEEGAMSNQQTESLKRILGERNLTEL 121
Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
SGEDHKRVRGALV FL+P +L+ YVGKMDGE+R+HL MYWH N EVTV PLMK LTFDII
Sbjct: 122 SGEDHKRVRGALVSFLKPDSLKKYVGKMDGEIRKHLQMYWHDNKEVTVLPLMKILTFDII 181
Query: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
CSLLFGIEEG RKS++ CFKTMVDGIWS+PINLPFTRY+HSLKASAK QQ+LKQLLK+K
Sbjct: 182 CSLLFGIEEGPRRKSMVGCFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQMLKQLLKEK 241
Query: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
+ ME+E + EE +DL++ LL +KNKDKEQALSE+EIVHNII+LM+AGHDTT+IL+T
Sbjct: 242 REKMEKEDQ---EEEQQDLVTCLLSMKNKDKEQALSEDEIVHNIIILMVAGHDTTTILIT 301
Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360
LM+RVL +NP VYAAVLQEHEEI RSKERGE L+WEDV KMKYTWRVA+ETLRL+PPVFG
Sbjct: 302 LMIRVLGSNPAVYAAVLQEHEEIARSKERGEPLSWEDVGKMKYTWRVALETLRLFPPVFG 361
Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
GFRVAL+DI+ G YTIPKGWQ+FWAAPMTHLD+TIF DP KFEP+RFD+ QT IPP FI
Sbjct: 362 GFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDDTIFADPHKFEPNRFDK-QTLIPPLCFI 421
Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
AFGGGPRICPG EFAKLETL+TIHYLITQFTW L CS++FLTRDPTLMPNKGLPIQI+PK
Sbjct: 422 AFGGGPRICPGSEFAKLETLITIHYLITQFTWKL-CSEEFLTRDPTLMPNKGLPIQIYPK 472
BLAST of CsaV3_3G022960 vs. TAIR 10
Match:
AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )
HSP 1 Score: 324.7 bits (831), Expect = 1.2e-88
Identity = 179/486 (36.83%), Postives = 285/486 (58.64%), Query Frame = 0
Query: 1 MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
++ ++ L + + L R LS + NLPPG G+P IG+S S L A R EK++
Sbjct: 5 IMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEKFIT 64
Query: 61 KRVDKYGP----VSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRN 120
RV ++ V K LFG PT + GA+ NK +F+ E V + +S+ I
Sbjct: 65 DRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNK-FLFTNENKLVVSWWPDSVNKIFPSSM 124
Query: 121 LTELSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLT 180
T S E+ +++R L F++P+ LR YVG MD +RH W ++V V PL K T
Sbjct: 125 QTS-SKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFT 184
Query: 181 FDIICSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQL 240
F I C +E+ + + E F T+ GI+SIPI+LP TR+N ++KAS ++ + +
Sbjct: 185 FSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAI 244
Query: 241 LKDKAKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTS 300
++ + + ++ K E + D++S++L + K +E++ II L+I GHDT S
Sbjct: 245 VRQRKEELKAGKALE----EHDILSHMLMNIGETK-----DEDLADKIIGLLIGGHDTAS 304
Query: 301 ILLTLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYP 360
I+ T ++ LA P VY VLQE +EI + K+ E L WED+ KM+Y+W VA E +R+ P
Sbjct: 305 IVCTFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVP 364
Query: 361 PVFGGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPP 420
P+ G FR A+ + IPKGW+++W+A TH++ F +P++FEP+RF+ + P
Sbjct: 365 PLSGTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGP--KP 424
Query: 421 FSFIAFGGGPRICPGYEFAKLETLVTIHYLITQFTW-NLSCSQDFLTRDPTLMPNKGLPI 480
++++ FGGGPR+CPG E+A+LE L+ +H L+ +F W + +++ + DP +P+KGLPI
Sbjct: 425 YTYVPFGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPI 477
Query: 481 QIFPKS 482
+IFP+S
Sbjct: 485 RIFPQS 477
BLAST of CsaV3_3G022960 vs. TAIR 10
Match:
AT2G42850.1 (cytochrome P450, family 718 )
HSP 1 Score: 319.7 bits (818), Expect = 4.0e-87
Identity = 169/475 (35.58%), Postives = 276/475 (58.11%), Query Frame = 0
Query: 11 TIFLF----HRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTA-EKWLQKRVDK 70
T+F F HRF T+ K L PG +G+P+IG+++ A ++N E ++ R+ K
Sbjct: 23 TLFFFRKKHHRFITK-KIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIK 82
Query: 71 YGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGEDH 130
+G + K + G PT+ ++GA AN+ ++ S E V + S ++G + GE H
Sbjct: 83 HGNIFKTRIMGSPTIVVNGAEANR-LILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKH 142
Query: 131 KRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLLF 190
+ +RG + L L S + K+ V+ H W G E+++ K LTF ++ L+
Sbjct: 143 RVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLY 202
Query: 191 GIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKAKVME 250
GI+ ++E F+ +++G++++P+ P +++ + KA + + L KV E
Sbjct: 203 GIK---VEIGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFL------VGKVRE 262
Query: 251 EEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLRV 310
+ +E E E +K + R+ + + ++EEE+V N++LL+ A HDTTS +++ ++
Sbjct: 263 KRREMEKEGAEKPNTTLFSRLVEELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFKM 322
Query: 311 LATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRVA 370
LA +PT +LQEH +I +K GE LT EDV KMKY+W+V ET+RL PP+FG FR A
Sbjct: 323 LAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKA 382
Query: 371 LKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFIAFGGG 430
+ DI G YTIPKGW+I W TH + IF DP F+P+RFD+ PI ++++ FGGG
Sbjct: 383 VADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDK---PIQAYTYLPFGGG 442
Query: 431 PRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 481
PR+C G++ AK+ LV +H+++T F W+L + ++ DP P+ G+PI+I PK
Sbjct: 443 PRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPK 483
BLAST of CsaV3_3G022960 vs. TAIR 10
Match:
AT5G05690.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 243.4 bits (620), Expect = 3.7e-64
Identity = 151/475 (31.79%), Postives = 246/475 (51.79%), Query Frame = 0
Query: 3 TVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKR 62
T +LL+ +I R + LPPG+LG+P IG++ L+GA +T E ++ +R
Sbjct: 4 TAFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDER 63
Query: 63 VDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSG 122
V +YG V LFG+PT+F N+ V E S+ +LGK +L + G
Sbjct: 64 VARYGSVFMTHLFGEPTIFSADPETNR-FVLQNEGKLFECSYPASICNLLGKHSLLLMKG 123
Query: 123 EDHKRVRGALVWFLRPQTLRSYVG-KMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIIC 182
HKR+ + F ++ ++ +D VR +L+ + ++ V + K +TF++
Sbjct: 124 SLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSW---SSRVLLMEEAKKITFELTV 183
Query: 183 SLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKA 242
L + G +S+ + + +++G +S+P+ L T Y +++A K + L ++
Sbjct: 184 KQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVV---- 243
Query: 243 KVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTL 302
+ E+E E E KD+++ LL + S+EEIV ++ L++AG++TTS ++TL
Sbjct: 244 -MKRREEEEEGAERKKDMLAALLA-----ADDGFSDEEIVDFLVALLVAGYETTSTIMTL 303
Query: 303 MLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGG 362
++ L P A + +EHE+I K +L W D M +T V ETLR+ + G
Sbjct: 304 AVKFLTETPLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGV 363
Query: 363 FRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFS-FI 422
FR A+ D+++ Y IPKGW++F + HLD F D + F P R+ N P + F
Sbjct: 364 FRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFT 423
Query: 423 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPI 476
FGGGPR+CPGYE A++ V +H L+T F+W + QD L PT K PI
Sbjct: 424 PFGGGPRLCPGYELARVALSVFLHRLVTGFSW-VPAEQDKLVFFPTTRTQKRYPI 463
BLAST of CsaV3_3G022960 vs. TAIR 10
Match:
AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )
HSP 1 Score: 238.0 bits (606), Expect = 1.5e-62
Identity = 150/457 (32.82%), Postives = 234/457 (51.20%), Query Frame = 0
Query: 21 RLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVDKYGPVSKMTLFGKPTV 80
R SSS LPPG +G P++G++ L + + + +YG V K + G P V
Sbjct: 27 RRSSSTKLPLPPGTMGYPYVGETFQLY----SQDPNVFFAAKQRRYGSVFKTHVLGCPCV 86
Query: 81 FIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGEDHKRVRGALVWFLRPQT 140
I A K V+ + + S + +LGK+ + G+ H ++R ++ P
Sbjct: 87 MISSPEAAKFVLVT-KSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDA 146
Query: 141 LRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLLFGIEEGTTRKSIIECF 200
+R+ V ++ + LN W G ++ MKT TF++ + G +E R+ + C+
Sbjct: 147 IRNMVPHIESIAQESLNS-WDG-TQLNTYQEMKTYTFNVALISILGKDEVYYREDLKRCY 206
Query: 201 KTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKAKVMEEEKESEVEENDKDLI 260
+ G S+PINLP T ++ ++KA + QIL +L K + + DL+
Sbjct: 207 YILEKGYNSMPINLPGTLFHKAMKARKELAQILANIL---------SKRRQNPSSHTDLL 266
Query: 261 SYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLRVLATNPTVYAAVLQEH 320
+ + + L++E+I NII ++ A DTT+ +LT +L+ LA NPTV AV +E
Sbjct: 267 GSFM-----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQ 326
Query: 321 EEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRVALKDIQLGAYTIPKGW 380
I + K+ GE+LTWED KM T+RV ETLR + FR A++D++ Y IPKGW
Sbjct: 327 MAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGW 386
Query: 381 QIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFIAFGGGPRICPGYEFAKLETL 440
++ H + IF DP KF+PSRF + P +F+ FG G CPG E AKLE
Sbjct: 387 KVLPLFRNIHHNADIFSDPGKFDPSRF---EVAPKPNTFMPFGSGIHSCPGNELAKLEIS 446
Query: 441 VTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQI 478
V IH+L T++ W++ D + P +P GLPI +
Sbjct: 447 VLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIAL 459
BLAST of CsaV3_3G022960 vs. TAIR 10
Match:
AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )
HSP 1 Score: 232.6 bits (592), Expect = 6.5e-61
Identity = 159/484 (32.85%), Postives = 250/484 (51.65%), Query Frame = 0
Query: 5 IILLVLTIF---LFHRFRTRLSS-----SAAKNLPPGALGVPFIGQSLSLLGAMRTNTAE 64
I L LT+F LF F L S S+ LPPG +G P++G++ L +
Sbjct: 3 ISALFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY----SQDPN 62
Query: 65 KWLQKRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRN 124
+ Q + +YG V K + G P V I A K V+ + + S + +LGK+
Sbjct: 63 VFFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVT-KSHLFKPTFPASKERMLGKQA 122
Query: 125 LTELSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLT 184
+ G+ H ++R ++ P+++R+ V ++ + L W G + MKT T
Sbjct: 123 IFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRS-WEG-TMINTYQEMKTYT 182
Query: 185 FDIICSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQL 244
F++ +FG +E R+ + C+ + G S+P+NLP T ++ S+KA + QIL
Sbjct: 183 FNVALLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQIL--- 242
Query: 245 LKDKAKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTS 304
A+++ E +++ ND LL DKE+ L++E+I NII ++ A DTT+
Sbjct: 243 ----ARILSERRQNGSSHND------LLGSFMGDKEE-LTDEQIADNIIGVIFAARDTTA 302
Query: 305 ILLTLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYP 364
+++ +L+ LA NP V AV +E I + KE GE+LTW D KM T RV ETLR+
Sbjct: 303 SVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVAS 362
Query: 365 PVFGGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPP 424
+ FR A++D++ Y IPKGW++ H IF +P KF+PSRF + P
Sbjct: 363 ILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRF---EVAPKP 422
Query: 425 FSFIAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQ 481
+F+ FG G CPG E AKLE + IH+L T+++W++ + D + P +P GLPI
Sbjct: 423 NTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPIV 462
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_004152028.1 | 1.7e-276 | 100.00 | taxadiene 5-alpha hydroxylase [Cucumis sativus] >KGN57973.1 hypothetical protein... | [more] |
| XP_008454553.1 | 2.7e-258 | 93.35 | PREDICTED: LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like [Cucumis melo... | [more] |
| XP_038899676.1 | 9.5e-248 | 90.44 | taxadiene 5-alpha hydroxylase-like [Benincasa hispida] | [more] |
| XP_023535435.1 | 6.8e-230 | 84.49 | taxadiene 5-alpha hydroxylase-like [Cucurbita pepo subsp. pepo] | [more] |
| XP_022976494.1 | 7.5e-229 | 84.49 | taxadiene 5-alpha hydroxylase-like [Cucurbita maxima] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q6WG30 | 1.1e-94 | 38.99 | Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2 | [more] |
| Q84KI1 | 1.8e-92 | 37.27 | Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1 | [more] |
| Q50EK1 | 5.3e-92 | 38.96 | Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1 | [more] |
| Q50EK0 | 5.8e-91 | 38.96 | Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1 | [more] |
| Q9AXM6 | 5.8e-91 | 36.10 | Taxane 10-beta-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A1 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0L864 | 8.0e-277 | 100.00 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G416660 PE=3 SV=1 | [more] |
| A0A1S3C037 | 1.3e-258 | 93.35 | LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like OS=Cucumis melo OX=3656 ... | [more] |
| A0A6J1INM7 | 3.7e-229 | 84.49 | taxadiene 5-alpha hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111476875 P... | [more] |
| A0A6J1F7G6 | 4.0e-228 | 83.92 | taxadiene 5-alpha hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443055... | [more] |
| A0A6J1CCZ3 | 1.4e-207 | 75.63 | taxadiene 5-alpha hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC11101035... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT5G36110.1 | 1.2e-88 | 36.83 | cytochrome P450, family 716, subfamily A, polypeptide 1 | [more] |
| AT2G42850.1 | 4.0e-87 | 35.58 | cytochrome P450, family 718 | [more] |
| AT5G05690.1 | 3.7e-64 | 31.79 | Cytochrome P450 superfamily protein | [more] |
| AT5G45340.1 | 1.5e-62 | 32.82 | cytochrome P450, family 707, subfamily A, polypeptide 3 | [more] |
| AT4G19230.1 | 6.5e-61 | 32.85 | cytochrome P450, family 707, subfamily A, polypeptide 1 | [more] |
