CsaV3_2G035340 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_2G035340 vs. NCBI nr
Match: KAE8652309.1 (hypothetical protein Csa_022554 [Cucumis sativus])

HSP 1 Score: 696.4 bits (1796), Expect = 1.2e-196
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60
           MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS
Sbjct: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60

Query: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120
           NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN
Sbjct: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120

Query: 121 DGGNTMIHHLLTLQDDIILAGIDTSTVTLEWALSQLLNNPEILKKSKEEIDCLIGRERLV 180
           DGGNTMIHHLLTLQDDIILAGIDTSTVTLEWALSQLLNNPEILKKSKEEIDCLIGRERLV
Sbjct: 121 DGGNTMIHHLLTLQDDIILAGIDTSTVTLEWALSQLLNNPEILKKSKEEIDCLIGRERLV 180

Query: 181 SEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGYDTPRDTIVLIHAWAIHR 240
           SEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGYDTPRDTIVLIHAWAIHR
Sbjct: 181 SEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGYDTPRDTIVLIHAWAIHR 240

Query: 241 DSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPGVGMAQRVVGLALASLIQSFEW 300
           DSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPGVGMAQRVVGLALASLIQSFEW
Sbjct: 241 DSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPGVGMAQRVVGLALASLIQSFEW 300

Query: 301 KSHSLIDMSESKGLTMPKAQPWVAHCKPCPFHI 334
           KSHSLIDMSESKGLTMPKAQPWVAHCKPCPFHI
Sbjct: 301 KSHSLIDMSESKGLTMPKAQPWVAHCKPCPFHI 333

BLAST of CsaV3_2G035340 vs. NCBI nr
Match: XP_031737068.1 (cytochrome P450 81Q32-like [Cucumis sativus])

HSP 1 Score: 642.1 bits (1655), Expect = 2.6e-180
Identity = 313/333 (93.99%), Postives = 315/333 (94.59%), Query Frame = 0

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60
           MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS
Sbjct: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60

Query: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120
           NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQL++      N
Sbjct: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLMLKP-EFYN 120

Query: 121 DGGNTMIHHLLTLQDDIILAGIDTSTVTLEWALSQLLNNPEILKKSKEEIDCLIGRERLV 180
           D      H    L  DIILAGIDTSTVTLEWALSQLLNNPEILKKSKEEIDCLIGRERLV
Sbjct: 121 D------HAFKVLNQDIILAGIDTSTVTLEWALSQLLNNPEILKKSKEEIDCLIGRERLV 180

Query: 181 SEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGYDTPRDTIVLIHAWAIHR 240
           SEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGYDTPRDTIVLIHAWAIHR
Sbjct: 181 SEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGYDTPRDTIVLIHAWAIHR 240

Query: 241 DSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPGVGMAQRVVGLALASLIQSFEW 300
           DSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPGVGMAQRVVGLALASLIQSFEW
Sbjct: 241 DSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPGVGMAQRVVGLALASLIQSFEW 300

Query: 301 KSHSLIDMSESKGLTMPKAQPWVAHCKPCPFHI 334
           KSHSLIDMSESKGLTMPKAQPWVAHCKPCPFHI
Sbjct: 301 KSHSLIDMSESKGLTMPKAQPWVAHCKPCPFHI 326

BLAST of CsaV3_2G035340 vs. NCBI nr
Match: XP_008455824.1 (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])

HSP 1 Score: 511.5 bits (1316), Expect = 5.4e-141
Identity = 254/348 (72.99%), Postives = 286/348 (82.18%), Query Frame = 0

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60
           MFL IREDEVK LL KL GNHCKLEDD FRVVE+GPLLKDLTLNIVMRMVSGK+FYEEDS
Sbjct: 142 MFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLKDLTLNIVMRMVSGKMFYEEDS 201

Query: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120
           NKFREIVT+IMEH GAS+P DFIPLWNWIDPTGFMKKVKKLGKTSDEFLQ LIDGIRNQN
Sbjct: 202 NKFREIVTQIMEHAGASNPGDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQQLIDGIRNQN 261

Query: 121 DGGNTMIHHLLTLQD----------------DIILAGIDTSTVTLEWALSQLLNNPEILK 180
           DGGNTMIHHLLTLQ+                DI+LAGIDTS VTL+WALS LLNNP +L+
Sbjct: 262 DGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQDILLAGIDTSAVTLQWALSHLLNNPIVLE 321

Query: 181 KSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGYD 240
           K+K EID  IG+ER+V+EADL +LSYLQGII +TL L P  PLLVPHC+  E+CKI GYD
Sbjct: 322 KAKAEIDSYIGQERMVNEADLSSLSYLQGIISETLRLSPAGPLLVPHCS-SEDCKIGGYD 381

Query: 241 TPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGE-LYKLIPFGVGRRACPGVGMA 300
            PR+TIVLI+AWAIHRD +LWE+ + FKPERH +    E  YKL+PFG+GRRACPG+ MA
Sbjct: 382 VPRNTIVLINAWAIHRDPNLWEDASSFKPERHVNAAGFENSYKLLPFGMGRRACPGMAMA 441

Query: 301 QRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPC 330
           QRV+GL LASL+Q FEWK  S  L+DM E +GLTMPK +P VA C+PC
Sbjct: 442 QRVIGLTLASLVQCFEWKRMSDLLVDMREGEGLTMPKVEPLVAKCRPC 488

BLAST of CsaV3_2G035340 vs. NCBI nr
Match: XP_008455822.2 (PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 473.8 bits (1218), Expect = 1.2e-129
Identity = 244/351 (69.52%), Postives = 279/351 (79.49%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDSN 61
           F + RE+EVK LL KL GN+ KLED+ FRVVE+ P+L DLT NIVMRMV GK F EE +N
Sbjct: 144 FSRTREEEVKRLLRKLWGNY-KLEDE-FRVVELEPMLLDLTFNIVMRMVGGKKFCEEKNN 203

Query: 62  ----------KFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQL 121
                     +F+E+VT+IM HGG+++P DFIPL NWIDP+G+ K + K+G+ +DE LQ 
Sbjct: 204 NVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPSGYTKNIMKIGRKTDEVLQG 263

Query: 122 LIDGIRNQNDGGNTMIHHLLTLQ----------------DDIILAGIDTSTVTLEWALSQ 181
           L+D IRN+ D GNTMI HLL LQ                 DIILAGIDTST++LEWALSQ
Sbjct: 264 LVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQ 323

Query: 182 LLNNPEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALF 241
           LLNNPE+LKK+KEEIDCLIG +RLVSE DLP+LSYLQGII +TL LHPPAPLLVPHCALF
Sbjct: 324 LLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALF 383

Query: 242 EECKIKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRR 301
           EECKIK Y+ PRDTIVLI+AWAIHRDS LWE+ T+FKPERH SVIE ELYKLIPFGVGRR
Sbjct: 384 EECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERH-SVIESELYKLIPFGVGRR 443

Query: 302 ACPGVGMAQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVA 325
           ACPG GMAQRVVGLALASLIQSFEW+    SLIDMSE KGLTMPKAQP +A
Sbjct: 444 ACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIA 491

BLAST of CsaV3_2G035340 vs. NCBI nr
Match: XP_038901939.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 451.4 bits (1160), Expect = 6.6e-123
Identity = 224/304 (73.68%), Postives = 253/304 (83.22%), Query Frame = 0

Query: 47  MRMVSGKVFYEED-SNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTS 106
           MRMV GK FYEED SNKFRE+VT+IM HGGA++P DFIPLWNWIDPTGFMK+VKKL KTS
Sbjct: 1   MRMVCGKKFYEEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLAKTS 60

Query: 107 DEFLQLLIDGIRNQNDGGNTMIHHLLTLQD----------------DIILAGIDTSTVTL 166
           DEFLQ LID IRNQNDGGNTMIHHLL+LQ+                DI+LAGIDT+ VTL
Sbjct: 61  DEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTAVTL 120

Query: 167 EWALSQLLNNPEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLV 226
           EW LSQLLNNPE+L+K+K EID  IG ERLV+EA+LP+LSYLQGII ++L L+PP PLLV
Sbjct: 121 EWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVPLLV 180

Query: 227 PHCALFEECKIKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGEL-YKLI 286
           PHCALFEECKI+GYD PR+TI+LI+ WAIHRDSS+WE+ T FKPERH S IE +  YKLI
Sbjct: 181 PHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYSYKLI 240

Query: 287 PFGVGRRACPGVGMAQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHC 331
           PFG GRRACPGVGMAQRVVGL LASLIQSFEWK  ++SLIDM+E KGLTMPK +P VA C
Sbjct: 241 PFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLVAKC 300

BLAST of CsaV3_2G035340 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.6e-87
Identity = 173/350 (49.43%), Postives = 224/350 (64.00%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEED-- 61
           FL IREDEVK LL  L+    +     F  VEM   L +L+ N+ MRMV+GK ++ +D  
Sbjct: 145 FLSIREDEVKQLLLSLY----QSSGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDVD 204

Query: 62  ---SNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGI 121
              +  FR ++ ++ EH GAS+P DF+P   WID   + KKV K+ +  D FLQ LI   
Sbjct: 205 SDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSKISQEMDAFLQRLIHES 264

Query: 122 RNQNDGGNTMIHHLLTLQDD----------------IILAGIDTSTVTLEWALSQLLNNP 181
           R  N    TMI HLL+LQ+                 ++LAG DTS VT+EWA+S LLN+P
Sbjct: 265 R-INKNNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHP 324

Query: 182 EILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKI 241
           E L+K++ EI+  +G  RL+ E DLP L+YL  II +T  L P AP+LVPH    ++CK+
Sbjct: 325 ETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPAAPMLVPH-ESSDDCKV 384

Query: 242 KGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPGV 301
           +GYD P+ TI+L++AWAIHRD   W+  T FKPERH  V E E  KL+PFG+GRR+CPG 
Sbjct: 385 QGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGV-ELEPSKLMPFGMGRRSCPGS 444

Query: 302 GMAQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKP 329
           G+AQRVVGL L +LIQ FEWK    + IDM+E  GLTMPKAQP  A CKP
Sbjct: 445 GLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCKP 487

BLAST of CsaV3_2G035340 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.2e-81
Identity = 163/354 (46.05%), Postives = 225/354 (63.56%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           FL +R DEV+ L+S+L     +L      VVE+ P+L DLT N +MRM++GK +Y     
Sbjct: 152 FLYVRTDEVRRLISRL----SRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEETT 211

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            EE++ + R++V  +  +  + +  D++P+      + +  +VKKLG+ +D+FLQ LID 
Sbjct: 212 DEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENRVKKLGEETDKFLQGLIDD 271

Query: 122 IRNQNDGGNTMIHHLLTLQDD----------------IILAGIDTSTVTLEWALSQLLNN 181
            R Q + G TMI HLL LQ                  +++AG +TS VTLEWALS LLN+
Sbjct: 272 KRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNH 331

Query: 182 PEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECK 241
           P+++ K+++EID  +G +RL+ EADL  L YL+ I+ +TL LHP  PLLVPH A  E+CK
Sbjct: 332 PDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMA-SEDCK 391

Query: 242 IKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPG 301
           I  YD PR T +L++AWAIHRD + W++   FKPER     E E  KL+ FG+GRRACPG
Sbjct: 392 IGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK--EEEAQKLLAFGLGRRACPG 451

Query: 302 VGMAQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPCPF 332
            G+AQR+VGLAL SLIQ FEW+   +  +DM E  G T+PKA P  A CK  PF
Sbjct: 452 SGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPF 496

BLAST of CsaV3_2G035340 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 5.5e-80
Identity = 167/354 (47.18%), Postives = 223/354 (62.99%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           F+ IR+DE++ L+  L     K    GF  VEM PL   LT+N ++RMV+GK FY     
Sbjct: 154 FVSIRQDEIRRLIICL----AKNSQHGFVKVEMKPLFMCLTINNIIRMVAGKRFYGDGTE 213

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            + ++   R+++ +++  GGA +  D+ P+  ++  T + K VKKL    DEFLQ L++ 
Sbjct: 214 NDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEKHVKKLAGRVDEFLQSLVNE 273

Query: 122 IRNQNDGGNTMIHHLLTLQDD----------------IILAGIDTSTVTLEWALSQLLNN 181
            R +   GNTMI HLL+LQ+                 +ILAG DTS  TLEWA+S LLN+
Sbjct: 274 KRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNH 333

Query: 182 PEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECK 241
           PE+L+K+K EID  IG +RLV E D+  L YLQ I+ +TL L+P AP+L+PH A  E+C 
Sbjct: 334 PEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLA-SEDCI 393

Query: 242 IKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPG 301
           + GYD PR TI+L++AWAIHRD  LWE   KFKPER     +GE  KL+PFG+GRR+CPG
Sbjct: 394 VDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK--KGEDKKLMPFGIGRRSCPG 453

Query: 302 VGMAQRVVGLALASLIQSFEWK--SHSLIDMSES-KGLTMPKAQPWVAHCKPCP 331
            G+AQR+V LAL SL+Q FEW+      +DM ES KG TM KA    A CK  P
Sbjct: 454 SGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKARP 498

BLAST of CsaV3_2G035340 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 298.1 bits (762), Expect = 1.2e-79
Identity = 158/355 (44.51%), Postives = 225/355 (63.38%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEED-- 61
           F  IR DE + L+++L  +        F  +E+   L D+T N +MRM+SGK +Y ED  
Sbjct: 144 FSGIRRDETQRLITRLADD----SSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCD 203

Query: 62  ------SNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLI 121
                 +++FR++V+++++  GA++  DF+PL  ++D     K++K +   +D FL+ LI
Sbjct: 204 TSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLI 263

Query: 122 DGIRNQNDGGNTMIHHLLTLQDD----------------IILAGIDTSTVTLEWALSQLL 181
           +  R + +  NTMI HLL LQD                 ++LAG D+S VTLEW++S LL
Sbjct: 264 EEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLL 323

Query: 182 NNPEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEE 241
           N+PE+LKK K+E+D  +G++RLV E+DLP L+YL+ +I +TL L+ PAPLL+PH +  +E
Sbjct: 324 NHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPH-STSDE 383

Query: 242 CKIKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRAC 301
           C I GY  P+DTIVLI+AWAIHRD  LW   T FKPER     +GEL KLI FG+GRRAC
Sbjct: 384 CNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEK--KGELEKLIAFGMGRRAC 443

Query: 302 PGVGMAQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPCP 331
           PG G+A R + + LA LIQ F+WK  +   ID++E  G T+ K  P  A CK  P
Sbjct: 444 PGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLKAMCKSRP 491

BLAST of CsaV3_2G035340 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 7.9e-79
Identity = 157/357 (43.98%), Postives = 224/357 (62.75%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDG--FRVVEMGPLLKDLTLNIVMRMVSGKVFYEED 61
           F  IR DE + L+ KL       ED    F  VE+     D+T N +MRM+SGK +Y +D
Sbjct: 144 FAPIRRDETQRLIKKL------AEDSSTKFAEVELTFRFFDMTFNNIMRMISGKRYYGDD 203

Query: 62  --------SNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQL 121
                   +++FR++V+++++  GA++  DF+PL  ++D     K+VK++G+ +D FL  
Sbjct: 204 CDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRIGEKNDVFLSG 263

Query: 122 LIDGIRNQNDGGNTMIHHLLTLQDD----------------IILAGIDTSTVTLEWALSQ 181
           L+   R++ +  NTMI HLL +Q+                 ++LAG D+S VTLEW +S 
Sbjct: 264 LLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAGTDSSAVTLEWTMSN 323

Query: 182 LLNNPEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALF 241
           +LN PE+LKK ++E+D  +G++RLV E+DLP L+YL+ +I++TL L+ PAPLL+PH +  
Sbjct: 324 ILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLYTPAPLLLPH-STA 383

Query: 242 EECKIKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRR 301
           +EC + GY  PRDTIVLI+AWAIHRD   W   T FKPER     +GEL K+I FG+GRR
Sbjct: 384 DECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDK--KGELEKMIAFGMGRR 443

Query: 302 ACPGVGMAQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPCP 331
           ACPG G+A R + + LA L+Q F+WK  +   IDMSE  G TM K  P  A CK  P
Sbjct: 444 ACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTKLLPLKAMCKTRP 491

BLAST of CsaV3_2G035340 vs. ExPASy TrEMBL
Match: A0A1S3C323 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.6e-141
Identity = 254/348 (72.99%), Postives = 286/348 (82.18%), Query Frame = 0

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60
           MFL IREDEVK LL KL GNHCKLEDD FRVVE+GPLLKDLTLNIVMRMVSGK+FYEEDS
Sbjct: 142 MFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLKDLTLNIVMRMVSGKMFYEEDS 201

Query: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120
           NKFREIVT+IMEH GAS+P DFIPLWNWIDPTGFMKKVKKLGKTSDEFLQ LIDGIRNQN
Sbjct: 202 NKFREIVTQIMEHAGASNPGDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQQLIDGIRNQN 261

Query: 121 DGGNTMIHHLLTLQD----------------DIILAGIDTSTVTLEWALSQLLNNPEILK 180
           DGGNTMIHHLLTLQ+                DI+LAGIDTS VTL+WALS LLNNP +L+
Sbjct: 262 DGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQDILLAGIDTSAVTLQWALSHLLNNPIVLE 321

Query: 181 KSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGYD 240
           K+K EID  IG+ER+V+EADL +LSYLQGII +TL L P  PLLVPHC+  E+CKI GYD
Sbjct: 322 KAKAEIDSYIGQERMVNEADLSSLSYLQGIISETLRLSPAGPLLVPHCS-SEDCKIGGYD 381

Query: 241 TPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGE-LYKLIPFGVGRRACPGVGMA 300
            PR+TIVLI+AWAIHRD +LWE+ + FKPERH +    E  YKL+PFG+GRRACPG+ MA
Sbjct: 382 VPRNTIVLINAWAIHRDPNLWEDASSFKPERHVNAAGFENSYKLLPFGMGRRACPGMAMA 441

Query: 301 QRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPC 330
           QRV+GL LASL+Q FEWK  S  L+DM E +GLTMPK +P VA C+PC
Sbjct: 442 QRVIGLTLASLVQCFEWKRMSDLLVDMREGEGLTMPKVEPLVAKCRPC 488

BLAST of CsaV3_2G035340 vs. ExPASy TrEMBL
Match: A0A1S3C1S2 (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 6.0e-130
Identity = 244/351 (69.52%), Postives = 279/351 (79.49%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDSN 61
           F + RE+EVK LL KL GN+ KLED+ FRVVE+ P+L DLT NIVMRMV GK F EE +N
Sbjct: 144 FSRTREEEVKRLLRKLWGNY-KLEDE-FRVVELEPMLLDLTFNIVMRMVGGKKFCEEKNN 203

Query: 62  ----------KFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQL 121
                     +F+E+VT+IM HGG+++P DFIPL NWIDP+G+ K + K+G+ +DE LQ 
Sbjct: 204 NVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPSGYTKNIMKIGRKTDEVLQG 263

Query: 122 LIDGIRNQNDGGNTMIHHLLTLQ----------------DDIILAGIDTSTVTLEWALSQ 181
           L+D IRN+ D GNTMI HLL LQ                 DIILAGIDTST++LEWALSQ
Sbjct: 264 LVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQ 323

Query: 182 LLNNPEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALF 241
           LLNNPE+LKK+KEEIDCLIG +RLVSE DLP+LSYLQGII +TL LHPPAPLLVPHCALF
Sbjct: 324 LLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALF 383

Query: 242 EECKIKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRR 301
           EECKIK Y+ PRDTIVLI+AWAIHRDS LWE+ T+FKPERH SVIE ELYKLIPFGVGRR
Sbjct: 384 EECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERH-SVIESELYKLIPFGVGRR 443

Query: 302 ACPGVGMAQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVA 325
           ACPG GMAQRVVGLALASLIQSFEW+    SLIDMSE KGLTMPKAQP +A
Sbjct: 444 ACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIA 491

BLAST of CsaV3_2G035340 vs. ExPASy TrEMBL
Match: A0A6J1FTX9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448413 PE=3 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 4.2e-123
Identity = 226/350 (64.57%), Postives = 276/350 (78.86%), Query Frame = 0

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60
           +FL IRE+E+KCLL KL G+     +D FRVVE+ P+L DL  NIV RMV+GK FYE   
Sbjct: 142 LFLGIREEEIKCLLCKLCGDSL---EDRFRVVELEPMLLDLMFNIVTRMVAGKRFYER-H 201

Query: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120
           +KFRE+VT+IM H GA++P DFIPLW +IDP+GF K+VKKLGKTSDE LQ L+D IR++N
Sbjct: 202 DKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGKTSDELLQELVDEIRDKN 261

Query: 121 DGGNTMIHHLLTLQD----------------DIILAGIDTSTVTLEWALSQLLNNPEILK 180
           DGGNTMIH LL+LQ+                DI+LAGIDT  VTLEW +SQLLN+PE+L+
Sbjct: 262 DGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLAVTLEWTVSQLLNHPEVLE 321

Query: 181 KSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGYD 240
           K++ EID  IG ERLV+E+DLP+LSY+QGII +TL LHP APLL+PHCA FEECKI+GYD
Sbjct: 322 KARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPLLLPHCA-FEECKIEGYD 381

Query: 241 TPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIE--GELYKLIPFGVGRRACPGVGM 300
            PRDTI+ I+AWA+HRD +LWE+ T FKPERH + ++   E YKLIPFG+GRRACPG+GM
Sbjct: 382 VPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRRACPGLGM 441

Query: 301 AQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPCP 331
           AQRV GL LASLIQ FEW+  ++SL+DMSE KG+TMPKAQP VA CKP P
Sbjct: 442 AQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRP 486

BLAST of CsaV3_2G035340 vs. ExPASy TrEMBL
Match: A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.7e-122
Identity = 226/349 (64.76%), Postives = 266/349 (76.22%), Query Frame = 0

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEED- 60
           MF+ IRE+EVK LL KL G+      + FR VE+ P+L DLT N+VM MV G+ FY+E  
Sbjct: 142 MFVGIRENEVKRLLRKLCGDW----SEEFREVELEPMLSDLTFNVVMGMVGGRRFYDEGY 201

Query: 61  SNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQ 120
           S KFR++VT+IM HGG+S+P DFIPLWNW+DPTGF K++  L +  DE LQ LID  R+ 
Sbjct: 202 SKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDELLQELIDEARDG 261

Query: 121 NDGGNTMIHHLLTLQDD----------------IILAGIDTSTVTLEWALSQLLNNPEIL 180
            DGGNTMIHHLL LQ D                I LAGIDTS VTLEWALS LLNNP++L
Sbjct: 262 KDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSAVTLEWALSHLLNNPDVL 321

Query: 181 KKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGY 240
           KK++ EID LIG+ERLV+E+DLPNL YLQGII +TL L+P APLLVPHCA F++CKI+GY
Sbjct: 322 KKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPAAPLLVPHCA-FQDCKIEGY 381

Query: 241 DTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPGVGMA 300
           D PRDTIVLI+ WAIHRD +LWE+ T FKPERH      + YKL+PFG+GRRACPGVGMA
Sbjct: 382 DVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDTSGADSYKLLPFGLGRRACPGVGMA 441

Query: 301 QRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPCP 331
           QRVVGLALASLIQ FEW+  S SL+DMSE +GLTMPKAQP VA CKP P
Sbjct: 442 QRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAKCKPRP 485

BLAST of CsaV3_2G035340 vs. ExPASy TrEMBL
Match: A0A6J1JDS1 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111483622 PE=3 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 4.6e-122
Identity = 226/349 (64.76%), Postives = 272/349 (77.94%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDSN 61
           FL +R +E+KCLL KL GN   L +D FRVVE+ P+L DL  NIVMRM++GK  +E  S 
Sbjct: 143 FLGVRGEEIKCLLCKLCGN---LLEDRFRVVELEPMLLDLMFNIVMRMLAGKKSFEGHSY 202

Query: 62  KFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQND 121
           KF+E+VT IM H GA++P DFIPLW +IDPTGF K+ KKL KTSDE LQ L+D IR++ D
Sbjct: 203 KFKEMVTHIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLAKTSDELLQELLDEIRDKED 262

Query: 122 GGNTMIHHLLTLQD----------------DIILAGIDTSTVTLEWALSQLLNNPEILKK 181
           GGNTMIH LL+LQ+                DI+LAGIDT  VTLEWALSQLLNNPE+L+K
Sbjct: 263 GGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLVVTLEWALSQLLNNPEVLEK 322

Query: 182 SKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKGYDT 241
           ++ EID  IG ERLV+EADLP+LSY+QGII +TL LHP APLL+PHCA  EECKI+GYD 
Sbjct: 323 ARAEIDSSIGYERLVNEADLPSLSYVQGIISETLRLHPAAPLLLPHCA-SEECKIEGYDV 382

Query: 242 PRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIE--GELYKLIPFGVGRRACPGVGMA 301
           PRDTI+LI+AWA+HRD +LWE+ T FKPERH + ++   E YKLIPFG+GRRACPG+GMA
Sbjct: 383 PRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRRACPGLGMA 442

Query: 302 QRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPCP 331
           QRV GL LASLIQ FEW+  ++SL+DMSE KG+TMPKAQP VA CKP P
Sbjct: 443 QRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRP 487

BLAST of CsaV3_2G035340 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 307.4 bits (786), Expect = 1.4e-83
Identity = 162/352 (46.02%), Postives = 225/352 (63.92%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           FL IR+DE++ L+ +L  N        F  V+M  +L DLT N ++RMV+GK +Y     
Sbjct: 145 FLSIRKDEIRRLVFRLSRNF----SQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGVE 204

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            + ++ + R+++  ++   GA +  D++P+   +  + +  +VKKL    DEFLQ L+D 
Sbjct: 205 DDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLAGRLDEFLQGLVDE 264

Query: 122 IRNQNDGGNTMIHHLLTLQDD----------------IILAGIDTSTVTLEWALSQLLNN 181
            R   + GNTMI HLLTLQ+                 +ILAG DTS VTLEWALS +LN+
Sbjct: 265 KREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAVTLEWALSNVLNH 324

Query: 182 PEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECK 241
           P++L K+++EID  IG +RL+ E+D+ NL YLQ I+ +TL L+P AP+L+PH A  E+CK
Sbjct: 325 PDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVA-SEDCK 384

Query: 242 IKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPG 301
           + GYD PR TI+L + WAIHRD  LW++   FKPER     EGE  KL+PFG+GRRACPG
Sbjct: 385 VAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK--EGEAQKLMPFGLGRRACPG 444

Query: 302 VGMAQRVVGLALASLIQSFEW-KSHSLIDMSESKGLTMPKAQPWVAHCKPCP 331
            G+A R++ L L SLIQ  EW K    +DMSE KG+TMPKA+P  A C+  P
Sbjct: 445 SGLAHRLINLTLGSLIQCLEWEKIGEEVDMSEGKGVTMPKAKPLEAMCRARP 487

BLAST of CsaV3_2G035340 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 304.7 bits (779), Expect = 9.3e-83
Identity = 163/355 (45.92%), Postives = 223/355 (62.82%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           F  IR DE++ L+ +L  N       GF  VEM  +  DLT N ++RM++GK +Y     
Sbjct: 145 FSSIRRDEIRRLIGRLLRN----SSYGFTKVEMKSMFSDLTFNNIIRMLAGKCYYGDGKE 204

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            + ++ + R ++ + M   G  +  D+IP+  WI  T    ++KKL    DEFLQ L+D 
Sbjct: 205 DDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWI--TYSETRIKKLAGRLDEFLQGLVDE 264

Query: 122 IR-NQNDGGNTMIHHLLTLQD----------------DIILAGIDTSTVTLEWALSQLLN 181
            R  +    NTM+ HLL LQ+                 +I  G DT+ VTLEWALS LLN
Sbjct: 265 KREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLN 324

Query: 182 NPEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEEC 241
           NPE+L K+++EID +IG +RL+ E+D+PNL YLQ I+ +TL L+P AP+L+PH A  ++C
Sbjct: 325 NPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVA-SKDC 384

Query: 242 KIKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACP 301
           K+ GYD PR T++L +AWAIHRD  LW++ T FKPER     EGE  KL+PFG+GRRACP
Sbjct: 385 KVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEK--EGEAKKLMPFGLGRRACP 444

Query: 302 GVGMAQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPCPF 332
           G G+AQR+V L+L SLIQ FEW+      +DM+E  GLTMPKA+P  A C+   F
Sbjct: 445 GSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRARDF 490

BLAST of CsaV3_2G035340 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 303.9 bits (777), Expect = 1.6e-82
Identity = 163/354 (46.05%), Postives = 225/354 (63.56%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           FL +R DEV+ L+S+L     +L      VVE+ P+L DLT N +MRM++GK +Y     
Sbjct: 152 FLYVRTDEVRRLISRL----SRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEETT 211

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            EE++ + R++V  +  +  + +  D++P+      + +  +VKKLG+ +D+FLQ LID 
Sbjct: 212 DEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENRVKKLGEETDKFLQGLIDD 271

Query: 122 IRNQNDGGNTMIHHLLTLQDD----------------IILAGIDTSTVTLEWALSQLLNN 181
            R Q + G TMI HLL LQ                  +++AG +TS VTLEWALS LLN+
Sbjct: 272 KRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNH 331

Query: 182 PEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECK 241
           P+++ K+++EID  +G +RL+ EADL  L YL+ I+ +TL LHP  PLLVPH A  E+CK
Sbjct: 332 PDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMA-SEDCK 391

Query: 242 IKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPG 301
           I  YD PR T +L++AWAIHRD + W++   FKPER     E E  KL+ FG+GRRACPG
Sbjct: 392 IGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK--EEEAQKLLAFGLGRRACPG 451

Query: 302 VGMAQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPCPF 332
            G+AQR+VGLAL SLIQ FEW+   +  +DM E  G T+PKA P  A CK  PF
Sbjct: 452 SGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPF 496

BLAST of CsaV3_2G035340 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 302.0 bits (772), Expect = 6.0e-82
Identity = 159/347 (45.82%), Postives = 222/347 (63.98%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDSN 61
           FL IR+DE++ L+S L  +       GF  VEM  LL +L  N  +RM++GK ++ ED++
Sbjct: 146 FLYIRKDEIRRLISHLSRDSL----HGFVEVEMKTLLTNLASNTTIRMLAGKRYFGEDND 205

Query: 62  K---FREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRN 121
                + +V++ +   GA +P D++ +  W+  + + K++K LG   D FLQ L+D  R 
Sbjct: 206 DAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIKNLGNRFDTFLQKLVDEKRA 265

Query: 122 QNDGGNTMIHHLLTLQD----------------DIILAGIDTSTVTLEWALSQLLNNPEI 181
           + + G TMI HLL LQD                 +I+AG DTS+VTLEWA+S LLN+PEI
Sbjct: 266 EKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEI 325

Query: 182 LKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEECKIKG 241
           LKK++ EID  +G +RLV E+D+ NLSYLQ I+ +TL ++P  PLL+PH +  E+CK+ G
Sbjct: 326 LKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMYPAVPLLLPHLS-SEDCKVGG 385

Query: 242 YDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRACPGVGM 301
           YD P  T+VL +AWA+HRD  +WE+   FKPER     EGE  KLI FG+GRRACPG G+
Sbjct: 386 YDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEK--EGEAEKLISFGMGRRACPGAGL 445

Query: 302 AQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCK 328
           A R++  AL SL+Q FEW+      +DM+E KG T+PKA P  A CK
Sbjct: 446 AHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAIPLRAMCK 483

BLAST of CsaV3_2G035340 vs. TAIR 10
Match: AT1G66540.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.7e-81
Identity = 164/356 (46.07%), Postives = 224/356 (62.92%), Query Frame = 0

Query: 2   FLKIREDEVKCLLSKL-HGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY---- 61
           F  IR DE++ L+++L    +  LE   F  VEM  +L +L  N ++RMV+GK +Y    
Sbjct: 30  FYTIRRDEIRRLIARLSRSPNASLE---FAKVEMNSMLSNLAFNNIIRMVTGKCYYGDGA 89

Query: 62  --EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLID 121
             + ++ + R+++ + M   GA    D +P+  WI  T F ++VKK+    DEF Q L+D
Sbjct: 90  EDDPEAKRVRQLIAEAMSCFGAGHAADHLPMLRWI--TDFERRVKKIAARLDEFFQRLVD 149

Query: 122 GIR-NQNDGGNTMIHHLLTLQD----------------DIILAGIDTSTVTLEWALSQLL 181
             R  +    NTMI HLL+LQ                  +ILAG DTS VTLEWALS LL
Sbjct: 150 EKRVAKEKKENTMIDHLLSLQVSQPEYYTDHTIKGTMLSLILAGTDTSAVTLEWALSSLL 209

Query: 182 NNPEILKKSKEEIDCLIGRERLVSEADLPNLSYLQGIIFDTLPLHPPAPLLVPHCALFEE 241
           NNPE+LKK ++EID  IG +RL+ E+D+PNL YLQ I+ +TL L+P  PLLVPH +  E+
Sbjct: 210 NNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHIS-SED 269

Query: 242 CKIKGYDTPRDTIVLIHAWAIHRDSSLWENVTKFKPERHTSVIEGELYKLIPFGVGRRAC 301
           CK+ GYD P  T++L++ WAIHRD  LW++   FKPER     EGE +KL+ FG+GRRAC
Sbjct: 270 CKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEK--EGETHKLLTFGLGRRAC 329

Query: 302 PGVGMAQRVVGLALASLIQSFEWK--SHSLIDMSESKGLTMPKAQPWVAHCKPCPF 332
           PG G+A+R+V L+L SLIQ FEW+      +DM+E  GLTMP+A P VA C+   F
Sbjct: 330 PGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRARAF 377