CsaV3_2G026160 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_2G026160 vs. NCBI nr
Match: XP_004143027.3 (subtilisin-like protease SBT3 [Cucumis sativus] >KGN62450.1 hypothetical protein Csa_018640 [Cucumis sativus])

HSP 1 Score: 1507.3 bits (3901), Expect = 0.0e+00
Identity = 757/757 (100.00%), Postives = 757/757 (100.00%), Query Frame = 0

Query: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSS 60
           MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSS
Sbjct: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSS 60

Query: 61  SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGL 120
           SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGL
Sbjct: 61  SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGL 120

Query: 121 SSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCN 180
           SSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCN
Sbjct: 121 SSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCN 180

Query: 181 NKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTAR 240
           NKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTAR
Sbjct: 181 NKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTAR 240

Query: 241 GVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATF 300
           GVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATF
Sbjct: 241 GVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATF 300

Query: 301 AAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLF 360
           AAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLF
Sbjct: 301 AAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLF 360

Query: 361 PLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIF 420
           PLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIF
Sbjct: 361 PLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIF 420

Query: 421 ISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARY 480
           ISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARY
Sbjct: 421 ISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARY 480

Query: 481 SSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAA 540
           SSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAA
Sbjct: 481 SSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAA 540

Query: 541 GVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNK 600
           GVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNK
Sbjct: 541 GVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNK 600

Query: 601 AIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVNSSD 660
           AIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVNSSD
Sbjct: 601 AIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVNSSD 660

Query: 661 SKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKI 720
           SKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKI
Sbjct: 661 SKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKI 720

Query: 721 AGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ 758
           AGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ
Sbjct: 721 AGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ 757

BLAST of CsaV3_2G026160 vs. NCBI nr
Match: XP_008444560.1 (PREDICTED: subtilisin-like protease SBT1.9 [Cucumis melo] >KAA0060951.1 subtilisin-like protease SBT1.9 [Cucumis melo var. makuwa])

HSP 1 Score: 1409.0 bits (3646), Expect = 0.0e+00
Identity = 704/764 (92.15%), Postives = 728/764 (95.29%), Query Frame = 0

Query: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLH---- 60
           MA RLYFWFSL PIFWLCPILTET NYIVHMNSAAMPKPFASRHSWYSATISS+LH    
Sbjct: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60

Query: 61  ---SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120
              SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH
Sbjct: 61  SSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120

Query: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTH 180
           SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESF DDGMSEIPS+WKGECESSTH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 180

Query: 181 FNVSFCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFG 240
           FNVSFCNNKLIGARFFNKGLI+  P ATIS+NSTRDTIGHGTHTST AAGSY+KEASFFG
Sbjct: 181 FNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFG 240

Query: 241 YGRGTARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDD 300
           YGRGTARGVAPRARVAIYKAIW+EGNSVSDVVAAIDQAISDGVDVISLSIGIDG+PLY+D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYND 300

Query: 301 PVAIATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVS 360
           PV+IATFAAVERGIFVATSAGNNGPQL TVHNGAPW+LNVAAGTMDRDFGGTITLSNGVS
Sbjct: 301 PVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 360

Query: 361 VLGSSLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTA 420
           VLGSSLFPLN T GLSPLPIVFMGGCQNLKKL+R GYKIVVCED+DGYSLTSQVDNVQTA
Sbjct: 361 VLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420

Query: 421 NVALGIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKP 480
            V LGIFISNIFDWDNLIQTPFPSIF+NPYHGNIIKDYIHKSSDPKAEV FHKTIL TKP
Sbjct: 421 KVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKP 480

Query: 481 APMVARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTS 540
           AP VARYSSRGPSQSCPFVLKPDIMAPGD ILASWPQN+PAMDVNSTPIY+KFNVISGTS
Sbjct: 481 APTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTS 540

Query: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGS 600
           MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTAD++DNTQT IKD GNNNKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGS 600

Query: 601 GHVNPNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660
           GHVNPNKA+DPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI
Sbjct: 601 GHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660

Query: 661 MIVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQK 720
           +I NSSDSKT KRKI GEFKRTLTKIGE+RATYEAKL GMKGFKVRVKP  L FKRKNQK
Sbjct: 661 IIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQK 720

Query: 721 LSFELKIAGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ 758
           LSFELKIAGSA ESN+VFGYLSWAEVGG HI+QSPIVV+GMR+Q
Sbjct: 721 LSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 764

BLAST of CsaV3_2G026160 vs. NCBI nr
Match: XP_038885776.1 (subtilisin-like protease SBT3 [Benincasa hispida])

HSP 1 Score: 1261.1 bits (3262), Expect = 0.0e+00
Identity = 632/761 (83.05%), Postives = 689/761 (90.54%), Query Frame = 0

Query: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSS 60
           MAA LYFWFSLIPI WLCPILTET NYIVHMNSAAMPKPFASRHSWYSATISS+LHSSSS
Sbjct: 1   MAAGLYFWFSLIPILWLCPILTETDNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60

Query: 61  SSS--SFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFL 120
           SSS  S  SKL H+YNHAI GF ASL+PS+LEALKNSPGY+SSVLDSSV VDTTHSSHFL
Sbjct: 61  SSSSASSSSKLTHSYNHAIDGFSASLSPSELEALKNSPGYVSSVLDSSVRVDTTHSSHFL 120

Query: 121 GLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSF 180
           GLSSN GLLPIS YGSDVIIGFVDTGIWP+ ESF DDGMSEIPS+WKGECE  THFN S 
Sbjct: 121 GLSSNFGLLPISNYGSDVIIGFVDTGIWPEGESFNDDGMSEIPSRWKGECERGTHFNASL 180

Query: 181 CNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGT 240
           CN KLIGARFFNKGL++  P  TIS+NSTRDTIGHGTHT+T AAGSY+KEASFFGYGRGT
Sbjct: 181 CNKKLIGARFFNKGLLAKFPNITISMNSTRDTIGHGTHTATIAAGSYVKEASFFGYGRGT 240

Query: 241 ARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIA 300
           ARGVAPRAR+AIYKAIWEEGN+VSDV+AAIDQAISDGVDVISLS+G+DGVPLY DPVAIA
Sbjct: 241 ARGVAPRARIAIYKAIWEEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYQDPVAIA 300

Query: 301 TFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSS 360
           TFAAVERGIFVAT+ GNNGPQL TVHNGAPW+LNVAAGTMDRDFGGTITLSNGV++LGSS
Sbjct: 301 TFAAVERGIFVATTTGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVTILGSS 360

Query: 361 LFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDG-YSLTSQVDNVQTANVAL 420
           LFPLN T  +SPLPIVFMGGCQNLKKL+R GYKI VC+D D  YSL+SQVDNVQ+ANVAL
Sbjct: 361 LFPLNSTMDVSPLPIVFMGGCQNLKKLKRIGYKIAVCDDKDEYYSLSSQVDNVQSANVAL 420

Query: 421 GIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMV 480
           G+FISNI DWDNLIQT FPSIFL+PY+GNIIKDYI KSSDPKAEV FHKTIL TKPAP V
Sbjct: 421 GVFISNISDWDNLIQTSFPSIFLSPYNGNIIKDYIKKSSDPKAEVNFHKTILGTKPAPSV 480

Query: 481 ARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCP 540
           ARYSSRGPS SCPFVLKPDIMAPGD ILASWPQNV AM V S PIY+KFN++SGTSMSCP
Sbjct: 481 ARYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMYVYSDPIYTKFNIMSGTSMSCP 540

Query: 541 HAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVN 600
           HAAGVAALLK  H  WSPAAIRSAMMTTAD++DN QT IKD GN N+FA+PLAMGSGH+N
Sbjct: 541 HAAGVAALLKVVHHGWSPAAIRSAMMTTADVVDNNQTSIKDIGNKNRFASPLAMGSGHIN 600

Query: 601 PNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVN 660
           PNKAIDP L+YD+GIQDYVN+LCALNYT++QI+ ITR DSN+CENPS+DLNYPSFI+IVN
Sbjct: 601 PNKAIDPGLVYDMGIQDYVNLLCALNYTKSQIQTITRLDSNDCENPSMDLNYPSFIIIVN 660

Query: 661 SSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFE 720
           +S+SKT K +I GEFKRTLTKIGE RATYEAKL GMKGFKV V P KL FKRKNQKLSF+
Sbjct: 661 TSNSKTSKGEILGEFKRTLTKIGEKRATYEAKLRGMKGFKVGVNPKKLEFKRKNQKLSFK 720

Query: 721 LKIAGSA---RESNIVFGYLSWAEVGGGHIIQSPIVVSGMR 756
           LKIAG+A   RE+++VFGYLSW EVGGGHI+QSPIVV+G+R
Sbjct: 721 LKIAGTARVKRENDMVFGYLSWVEVGGGHIVQSPIVVAGLR 761

BLAST of CsaV3_2G026160 vs. NCBI nr
Match: KAG6598336.1 (Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1172.5 bits (3032), Expect = 0.0e+00
Identity = 587/758 (77.44%), Postives = 658/758 (86.81%), Query Frame = 0

Query: 4   RLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSL------LHS 63
           R++FW SLI IF     LT+T NYIVHM+ AAMPKPFA+ HSWYSAT+SS+      L +
Sbjct: 6   RMFFWVSLIVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65

Query: 64  SSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHF 123
           ++SSSSS PSKLIH Y HAI+GF A+LTPSQL+ALKNSPGY+SSV DSSV  DTTHSS+F
Sbjct: 66  TTSSSSSSPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNF 125

Query: 124 LGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVS 183
           L LS N GLLPIS YGSDVIIGFVDTG+WP+SESF DDG+ +IPS+WKGECES THFN S
Sbjct: 126 LALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGIPKIPSRWKGECESGTHFNAS 185

Query: 184 FCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRG 243
            CN KLIG RFFNKGLI+  P  TIS+NSTRDT GHGTHTSTTAAGSY+KEASFFGYG+G
Sbjct: 186 LCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQG 245

Query: 244 TARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAI 303
           TARGVAPRARVAIYKAIW+EGN+VSDV+AAIDQAISDGVDVISLS+G+DGVPLY+DPVAI
Sbjct: 246 TARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAI 305

Query: 304 ATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGS 363
           ATFAA+ERGIFVATSAGN GPQ  TVH+GAPW+LNVAAGTMDRDFGGTITL+NGVSVLGS
Sbjct: 306 ATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGS 365

Query: 364 SLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVAL 423
           SLFPLN+   LSPLPIVFMG C NLKKL+R G+KIVVCEDSD YSL  QVDNV++A +A+
Sbjct: 366 SLFPLNLAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAV 425

Query: 424 GIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMV 483
           G+FISNI DWDNLIQT FPSIFLN YHGN+IKDYI +SS+PKA V FHKTI+ TKPAP V
Sbjct: 426 GVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSV 485

Query: 484 ARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCP 543
           ARYSSRGPS+SCPFVLKPDIMAPGD ILASWPQNV A DV S PIYSK+NV+SGTSM+CP
Sbjct: 486 ARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSGTSMACP 545

Query: 544 HAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVN 603
           HAAGVAALLKGAHP WSPAAIRSAMMTTADI+DNTQT IKD GN NK ATPLAMGSGHVN
Sbjct: 546 HAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVN 605

Query: 604 PNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVN 663
           PNKAIDP LIYD+GI+DY N+LCALNYT+NQI+ ITRS SN+CE P LDLNYPSFI+ VN
Sbjct: 606 PNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPSFIITVN 665

Query: 664 SSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFE 723
           +SDS+T + +IS EFKR +T IGE  ATY AK+T MKG  V V+PNKL FKRKNQ LSF+
Sbjct: 666 ASDSQTGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFK 725

Query: 724 LKIAGSA---RESNIVFGYLSWAEVGGGHIIQSPIVVS 753
           LKI G     RES++VFGYL+W EVGGGH +QSPIVV+
Sbjct: 726 LKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVA 763

BLAST of CsaV3_2G026160 vs. NCBI nr
Match: XP_022962414.1 (subtilisin-like protease SBT1.9 [Cucurbita moschata])

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 589/759 (77.60%), Postives = 657/759 (86.56%), Query Frame = 0

Query: 4   RLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLL-------H 63
           R++FW SL+ IF     LT+T NYIVHM+ AAMPKPFA+ HSWYSAT+SS+L        
Sbjct: 6   RMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65

Query: 64  SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSH 123
           ++SSSSSS PSKLIH Y HAI+GF ASLTPSQL+ALKNSPGY+SS+ DSSV  DTTHSS+
Sbjct: 66  TTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSN 125

Query: 124 FLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNV 183
           FL LS N GLLPIS YGSDVIIGFVDTG+WP+SESF DDG+S+IPS+WKGECES THFN 
Sbjct: 126 FLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNA 185

Query: 184 SFCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGR 243
           S CN KLIG RFFNKGLIS  P  TIS+NSTRDT GHGTHTSTTAAGSY+KEASFFGYG+
Sbjct: 186 SLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQ 245

Query: 244 GTARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVA 303
           GTARGVAPRARVAIYKAIW+EGN+VSDV+AAIDQAISDGVDVISLS+G+DGVPLY+DPVA
Sbjct: 246 GTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVA 305

Query: 304 IATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLG 363
           IATFAA+ERGIFVATSAGN GPQ  TVH+GAPW+LNVAAGTMDRDFGGTITL+NGVSVLG
Sbjct: 306 IATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLG 365

Query: 364 SSLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVA 423
           SSLFPLN    LSPLPIVFMG C NLKKL+R G+KIVVCEDSD YSL  QVDNV++A +A
Sbjct: 366 SSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIA 425

Query: 424 LGIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPM 483
           +G+FISNI DWDNLIQT FPSIFLN YHGN+IKDYI +SS+PKA V FHKTI+ TKPAP 
Sbjct: 426 VGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPS 485

Query: 484 VARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSC 543
           VARYSSRGPS+SCPFVLKPDIMAPGD ILASWPQNV A DV S PIYSKFNV+SGTSM+C
Sbjct: 486 VARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMAC 545

Query: 544 PHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHV 603
           PHAAGVAALLKGAHP WSPAAIRSAMMTTADI+DNTQT IKD GN NK ATPLAMGSGHV
Sbjct: 546 PHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHV 605

Query: 604 NPNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIV 663
           NPNKAIDP LIYD+GI+DY N+LCALNYT+NQI+ ITRS SN+CE   LDLNYPSFIM V
Sbjct: 606 NPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMTV 665

Query: 664 NSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSF 723
           N+SDS+T + ++S EFKR +T IGE  ATY AK+T MKG  V V+PNKL FKRKNQ LSF
Sbjct: 666 NASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSF 725

Query: 724 ELKIAGSA---RESNIVFGYLSWAEVGGGHIIQSPIVVS 753
           +LKI G     RES++VFGYL+W EVGGGH +QSPIVV+
Sbjct: 726 KLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVA 764

BLAST of CsaV3_2G026160 vs. ExPASy Swiss-Prot
Match: O82777 (Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 4.1e-184
Identity = 352/744 (47.31%), Postives = 486/744 (65.32%), Query Frame = 0

Query: 21  LTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSSSSSSFPS--KLIHTYNHAIS 80
           L +   YIVH++ + MP  F   H W+S+TI S+  S  SS   F S  KL+++Y++ + 
Sbjct: 21  LAQRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSIKASVPSSVDRFHSAPKLVYSYDNVLH 80

Query: 81  GFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVII 140
           GF A L+  +L ALK  PG++S+  D +V   TTH+S FL L+ + GL P S  G DVI+
Sbjct: 81  GFSAVLSKDELAALKKLPGFISAYKDRTVEPHTTHTSDFLKLNPSSGLWPASGLGQDVIV 140

Query: 141 GFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFNKGLISGLP 200
             +D+GIWP+S SF DDGM EIP +WKG C+  T FN S CN KLIGA +FNKG+++  P
Sbjct: 141 AVLDSGIWPESASFQDDGMPEIPKRWKGICKPGTQFNASMCNRKLIGANYFNKGILANDP 200

Query: 201 KATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRARVAIYKAIWEEG 260
              I++NS RDT GHGTH ++  AG++ K  S FGY  GTARGVAPRAR+A+YK  + EG
Sbjct: 201 TVNITMNSARDTDGHGTHCASITAGNFAKGVSHFGYAPGTARGVAPRARLAVYKFSFNEG 260

Query: 261 NSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATFAAVERGIFVATSAGNNGP 320
              SD++AA+DQA++DGVD+IS+S G   +PLY+D ++IA+F A+ +G+ V+ SAGN GP
Sbjct: 261 TFTSDLIAAMDQAVADGVDMISISYGYRFIPLYEDAISIASFGAMMKGVLVSASAGNRGP 320

Query: 321 QLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNITTGLSPLPIVF--- 380
            + +++NG+PW+L VA+G  DR F GT+TL NG+ + G SLFP       S  P+++   
Sbjct: 321 GIGSLNNGSPWILCVASGHTDRTFAGTLTLGNGLKIRGWSLFPARAFVRDS--PVIYNKT 380

Query: 381 MGGCQN---LKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIFISNIFDWDNLIQ 440
           +  C +   L ++      IV+C+D+  +S   Q+  +  A +   IFIS          
Sbjct: 381 LSDCSSEELLSQVENPENTIVICDDNGDFS--DQMRIITRARLKAAIFISEDPGVFRSAT 440

Query: 441 TPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARYSSRGPSQSCPFV 500
            P P + +N   G  + +Y+  S  P A +TF +T L TKPAP+VA  S+RGPS+S   +
Sbjct: 441 FPNPGVVVNKKEGKQVINYVKNSVTPTATITFQETYLDTKPAPVVAASSARGPSRSYLGI 500

Query: 501 LKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVI-SGTSMSCPHAAGVAALLKGAHP 560
            KPDI+APG  ILA++P NV A  + +  + S   ++ SGTSM+ PHAAG+AA+LK AHP
Sbjct: 501 SKPDILAPGVLILAAYPPNVFATSIGTNILLSTDYILESGTSMAAPHAAGIAAMLKAAHP 560

Query: 561 QWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNKAIDPDLIYDVG 620
           +WSP+AIRSAMMTTAD LDNT+  IKD  +NNK ATPL MG+GHV+PN+A+DP L+YD  
Sbjct: 561 EWSPSAIRSAMMTTADPLDNTRKPIKD-SDNNKAATPLDMGAGHVDPNRALDPGLVYDAT 620

Query: 621 IQDYVNVLCALNYTENQIRIITRSD-SNNCENPSLDLNYPSFIMIVNSSDSKTRKRKISG 680
            QDYVN+LC+LN+TE Q + I RS  S+NC NPS DLNYPSFI + +   + T    +  
Sbjct: 621 PQDYVNLLCSLNFTEEQFKTIARSSASHNCSNPSADLNYPSFIALYSIEGNFT---LLEQ 680

Query: 681 EFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKI--AGSARESN 740
           +FKRT+T +G+  ATY+AKL   K   + V P  L FK KN+K S+ L I   G   +S 
Sbjct: 681 KFKRTVTNVGKGAATYKAKLKAPKNSTISVSPQILVFKNKNEKQSYTLTIRYIGDEGQSR 740

Query: 741 IVFGYLSWAEVGGGHIIQSPIVVS 753
            V G ++W E  G H ++SPIV S
Sbjct: 741 NV-GSITWVEQNGNHSVRSPIVTS 755

BLAST of CsaV3_2G026160 vs. ExPASy Swiss-Prot
Match: Q9FHA4 (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 3.7e-164
Identity = 321/745 (43.09%), Postives = 462/745 (62.01%), Query Frame = 0

Query: 11  LIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSSSSSSFPSKLI 70
           L+  F++  +  ET  YI+HM+ +A P PF+   SW+S T++S++       ++   K+I
Sbjct: 9   LVFSFFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVI-------TNRKPKII 68

Query: 71  HTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPIS 130
           + Y  ++ GF A LT S+L+ LK+ PGY+S   D  V + TT S  F+GL+S  G  P+S
Sbjct: 69  YAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTWPVS 128

Query: 131 KYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFN 190
            YG+ ++IG +DTGIWPDS SF DDG+  +PSKWKG CE ++    S CN KLIGA+ FN
Sbjct: 129 NYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFNSS---SLCNKKLIGAKVFN 188

Query: 191 KGLISGLPKATIS----INSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRA 250
           KGL +  P    +     +S  DTIGHGTH +  AAG+++K AS+F Y +GTA G+AP A
Sbjct: 189 KGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIAPHA 248

Query: 251 RVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGI--------DGVPLYDDPVAIA 310
            +AIYKA WEEG   SDV+AAIDQAI DGV VISLS+G+        DG  L +DP+A+A
Sbjct: 249 HLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPIAVA 308

Query: 311 TFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSS 370
           +FAA+++G+FV TS GN+GP   ++ NGAPW++ V AGT+ R F GT+T  N VS    S
Sbjct: 309 SFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFSFPS 368

Query: 371 LFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALG 430
           LFP    +   P+  +  G  +N    +    +IVVC  ++  ++ S++  +++   A  
Sbjct: 369 LFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIRSTGAAAV 428

Query: 431 IFISN-IFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKS-SDPKAEVTFHKTILRTKPAPM 490
           + I++ + +  + I+  FP  F+   H   I+ Y   + ++  A++ F KT++ TKPAP 
Sbjct: 429 VLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKPAPE 488

Query: 491 VARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSC 550
           V  YSSRGP  S P +LKPDI+APG  IL++WP         + P++S FN+++GTSM+ 
Sbjct: 489 VGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTSMAA 548

Query: 551 PHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHV 610
           PH AGVAAL+K  HP WSP+AI+SA+MTTA  LDN                PLA+G+GHV
Sbjct: 549 PHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGAGHV 608

Query: 611 NPNKAIDPDLIYDVGIQDYVNVLC-ALNYTENQIRIITRSD-SNNCENPSLDLNYPSFIM 670
           + NK ++P LIYD   QD++N LC     +   I IITRS+ S+ C+ PS  LNYPS I 
Sbjct: 609 STNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPSIIA 668

Query: 671 IVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKL 730
              S  S  +       FKRTLT +GE + +Y  ++ G+KG  V V+P KL F  KN+KL
Sbjct: 669 YFTSDQSSPK------IFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNEKL 715

Query: 731 SFELKI-AGSARESNIVFGYLSWAE 739
           S+ +++ +    + N+V+G +SW +
Sbjct: 729 SYTVRLESPRGLQENVVYGLVSWVD 715

BLAST of CsaV3_2G026160 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 2.2e-145
Identity = 312/764 (40.84%), Postives = 442/764 (57.85%), Query Frame = 0

Query: 27  YIVHMNSAAMPKPFASRHSWYSATISSLLHSSSSSSSSFPSKLIHTYNHAISGFCASLTP 86
           YIVH++  A P  F +   WY+++++SL        +S P  +IHTY+    GF A LT 
Sbjct: 28  YIVHVDHEAKPSIFPTHFHWYTSSLASL--------TSSPPSIIHTYDTVFHGFSARLTS 87

Query: 87  SQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNH--GLLPISKYGSDVIIGFVDTG 146
                L + P  +S + +   H+ TT S  FLGL S    GLL  S +GSD++IG +DTG
Sbjct: 88  QDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTG 147

Query: 147 IWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFNKG--LISGLPKATI 206
           +WP+  SF D G+  +P KWKG+C +S  F  S CN KL+GARFF  G    +G    T 
Sbjct: 148 VWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETT 207

Query: 207 SINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSVS 266
              S RD+ GHGTHT++ +AG Y+  AS  GY  G A G+AP+AR+A YK  W  G   S
Sbjct: 208 EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDS 267

Query: 267 DVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATFAAVERGIFVATSAGNNGPQLET 326
           D++AA D A++DGVDVISLS+G   VP Y D +AI  F A++RGIFV+ SAGN GP   T
Sbjct: 268 DILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALT 327

Query: 327 VHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNITTGLSP---LPIVF---- 386
           V N APW+  V AGT+DRDF   + L NG  + G S++      GL P    P+V+    
Sbjct: 328 VTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYG---GPGLDPGRMYPLVYGGSL 387

Query: 387 MGGCQNLKKLRRTGY--------KIVVCEDSDGYSLTSQVDNVQTANVALGIFISN-IFD 446
           +GG      L   G         KIV+C+   G +  +    +   N  LG+ I+N +FD
Sbjct: 388 LGGDGYSSSLCLEGSLDPNLVKGKIVLCD--RGINSRATKGEIVRKNGGLGMIIANGVFD 447

Query: 447 WDNLIQT--PFPSIFLNPYHGNIIKDYIHKSS------DPKAEVTFHKTILRTKPAPMVA 506
            + L+      P+  +    G+ I+ YI +SS       P A + F  T L  +PAP+VA
Sbjct: 448 GEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVA 507

Query: 507 RYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPH 566
            +S+RGP+   P +LKPD++APG  ILA+WP  +    V S    ++FN++SGTSM+CPH
Sbjct: 508 SFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPH 567

Query: 567 AAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNP 626
            +G+AALLK AHP WSPAAIRSA++TTA  +DN+   + D    N  ++ +  GSGHV+P
Sbjct: 568 VSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT-SSVMDYGSGHVHP 627

Query: 627 NKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSL-----DLNYPSFI 686
            KA+DP L+YD+   DY+N LC  NYT   I  ITR  + +C+         +LNYPSF 
Sbjct: 628 TKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA-DCDGARRAGHVGNLNYPSFS 687

Query: 687 MIVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQK 746
           ++      +  + K+S  F RT+T +G+  + YE K+   +G  V V+P KL+F+R  QK
Sbjct: 688 VVF----QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQK 747

Query: 747 LSFELKIAG-----SARESNIVFGYLSWAEVGGGHIIQSPIVVS 753
           LSF +++       S   +N+  G++ W++  G   + SP+VV+
Sbjct: 748 LSFVVRVKTTEVKLSPGATNVETGHIVWSD--GKRNVTSPLVVT 770

BLAST of CsaV3_2G026160 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 6.5e-145
Identity = 298/756 (39.42%), Postives = 436/756 (57.67%), Query Frame = 0

Query: 22  TETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSSSSSSFPSKLIHTYNHAISGFC 81
           T  + YI+ +N +  P+ F + H WY++ ++S             S L++TY  +  GF 
Sbjct: 25  TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS------------ESSLLYTYTTSFHGFS 84

Query: 82  ASLTPSQLEALKNSPGYLSSVLDSSVH-VDTTHSSHFLGLSSNHGLLPISKYGSDVIIGF 141
           A L  ++ ++L +S   +  + +  ++ + TT +  FLGL+S  G+  +    + VIIG 
Sbjct: 85  AYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGV 144

Query: 142 VDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFNKGLI----SG 201
           +DTG+WP+S SF D  M EIPSKWKGECES + F+   CN KLIGAR F+KG       G
Sbjct: 145 LDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGG 204

Query: 202 LPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRARVAIYKAIWE 261
                 S+ S RD  GHGTHTSTTAAGS ++ ASF GY  GTARG+A RARVA YK  W 
Sbjct: 205 FSSKRESV-SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWS 264

Query: 262 EGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATFAAVERGIFVATSAGNN 321
            G   SD++AA+D+AI DGVDV+SLS+G    P Y D +AI  F+A+ERG+FV+ SAGN+
Sbjct: 265 TGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNS 324

Query: 322 GPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNITTGLSPLPIVFM 381
           GP   +V N APW++ V AGT+DRDF     L NG  + G SL+   +  G  PL +V+ 
Sbjct: 325 GPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKPLELVYN 384

Query: 382 GGCQNLKKLRRTGY--------KIVVCEDSDGYSLTSQVDNVQTANVALGIFISN-IFDW 441
            G  +   L   G         KIVVC+   G +   +   V      LG+ ++N     
Sbjct: 385 KGNSSSSNLCLPGSLDSSIVRGKIVVCD--RGVNARVEKGAVVRDAGGLGMIMANTAASG 444

Query: 442 DNLIQTP--FPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARYSSRGP 501
           + L+      P+I +    G+++++Y+   S P A + F  T+L  KP+P+VA +SSRGP
Sbjct: 445 EELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGP 504

Query: 502 SQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAAGVAAL 561
           +   P +LKPD++ PG  ILA W   +    ++     ++FN++SGTSMSCPH +G+A L
Sbjct: 505 NTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGL 564

Query: 562 LKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNKAIDPD 621
           LK AHP+WSP+AI+SA+MTTA +LDNT   + D  +N+  + P A GSGHV+P KA+ P 
Sbjct: 565 LKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNS-LSNPYAHGSGHVDPQKALSPG 624

Query: 622 LIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLD---LNYPSFIMIVNSSDSK 681
           L+YD+  ++Y+  LC+L+YT + I  I +  S NC     D   LNYPSF ++       
Sbjct: 625 LVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGG---- 684

Query: 682 TRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKIA- 741
             KR +   + R +T +G   + Y+  + G     + VKP+KL+FK   +K  + +    
Sbjct: 685 --KRVV--RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 744

Query: 742 --GSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMR 756
             G +  +   FG ++W+     H ++SP+  S  R
Sbjct: 745 KKGVSMTNKAEFGSITWS--NPQHEVRSPVAFSWNR 753

BLAST of CsaV3_2G026160 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 6.9e-139
Identity = 304/748 (40.64%), Postives = 429/748 (57.35%), Query Frame = 0

Query: 27  YIVHMNSAAMPKPFASRHSWYSATISSLLHSSSSSSSSFPSKLIHTYNHAISGFCASLTP 86
           YIVHM  + MP  F    +WY +++ S+  S         ++L++TY +AI GF   LT 
Sbjct: 32  YIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---------AELLYTYENAIHGFSTRLTQ 91

Query: 87  SQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHG-LLPISKYGSDVIIGFVDTGI 146
            + ++L   PG +S + +    + TT +  FLGL  +   L P +   SDV++G +DTG+
Sbjct: 92  EEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGV 151

Query: 147 WPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFNKGLIS--GLPKATIS 206
           WP+S+S+ D+G   IPS WKG CE+ T+F  S CN KLIGARFF +G  S  G    +  
Sbjct: 152 WPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKE 211

Query: 207 INSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSVSD 266
             S RD  GHGTHTS+TAAGS ++ AS  GY  GTARG+APRARVA+YK  W  G   SD
Sbjct: 212 SRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSD 271

Query: 267 VVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATFAAVERGIFVATSAGNNGPQLETV 326
           ++AAID+AI+D V+V+S+S+G      Y D VAI  FAA+ERGI V+ SAGN GP   ++
Sbjct: 272 ILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSL 331

Query: 327 HNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNITTGLSPLPIVFMGGCQNLK 386
            N APW+  V AGT+DRDF     L NG +  G SLF          LP ++ G   N  
Sbjct: 332 SNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD-KLLPFIYAGNASNAT 391

Query: 387 --KLRRTGY--------KIVVCEDSDGYSLTSQVDNVQTANVALGIFISN-IFDWDNLIQ 446
              L  TG         KIV+C+   G +   Q  +V  A   +G+ ++N   + + L+ 
Sbjct: 392 NGNLCMTGTLIPEKVKGKIVMCD--RGINARVQKGDVVKAAGGVGMILANTAANGEELVA 451

Query: 447 TP--FPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARYSSRGPSQSCP 506
                P+  +    G+II+ Y+    +P A ++   T++  KP+P+VA +SSRGP+   P
Sbjct: 452 DAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITP 511

Query: 507 FVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAAGVAALLKGAH 566
            +LKPD++APG  ILA+W        + S     +FN+ISGTSMSCPH +G+AALLK  H
Sbjct: 512 NILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVH 571

Query: 567 PQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNKAIDPDLIYDV 626
           P+WSPAAIRSA+MTTA         + D     K +TP   G+GHV+P  A +P LIYD+
Sbjct: 572 PEWSPAAIRSALMTTAYKTYKDGKPLLDIA-TGKPSTPFDHGAGHVSPTTATNPGLIYDL 631

Query: 627 GIQDYVNVLCALNYTENQIRIITRSDSNNCENPS---LDLNYPSFIMIVNSSDSKTRKRK 686
             +DY+  LCALNYT  QIR ++R +     + S    DLNYPSF + V+   +      
Sbjct: 632 TTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAY----- 691

Query: 687 ISGEFKRTLTKIGEHRATYEAKLTG-MKGFKVRVKPNKLNFKRKNQKLSFEL--KIAGSA 746
              ++ RT+T +G    TY  K+T    G K+ V+P  LNFK  N+K S+ +   +  S 
Sbjct: 692 ---KYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSK 751

Query: 747 RESNIVFGYLSWAEVGGGHIIQSPIVVS 753
              +  FG + W++  G H++ SP+ +S
Sbjct: 752 PSGSNSFGSIEWSD--GKHVVGSPVAIS 755

BLAST of CsaV3_2G026160 vs. ExPASy TrEMBL
Match: A0A0A0LKL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354760 PE=3 SV=1)

HSP 1 Score: 1507.3 bits (3901), Expect = 0.0e+00
Identity = 757/757 (100.00%), Postives = 757/757 (100.00%), Query Frame = 0

Query: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSS 60
           MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSS
Sbjct: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSS 60

Query: 61  SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGL 120
           SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGL
Sbjct: 61  SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGL 120

Query: 121 SSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCN 180
           SSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCN
Sbjct: 121 SSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCN 180

Query: 181 NKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTAR 240
           NKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTAR
Sbjct: 181 NKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTAR 240

Query: 241 GVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATF 300
           GVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATF
Sbjct: 241 GVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATF 300

Query: 301 AAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLF 360
           AAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLF
Sbjct: 301 AAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLF 360

Query: 361 PLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIF 420
           PLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIF
Sbjct: 361 PLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIF 420

Query: 421 ISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARY 480
           ISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARY
Sbjct: 421 ISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARY 480

Query: 481 SSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAA 540
           SSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAA
Sbjct: 481 SSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAA 540

Query: 541 GVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNK 600
           GVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNK
Sbjct: 541 GVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNK 600

Query: 601 AIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVNSSD 660
           AIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVNSSD
Sbjct: 601 AIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVNSSD 660

Query: 661 SKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKI 720
           SKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKI
Sbjct: 661 SKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKI 720

Query: 721 AGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ 758
           AGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ
Sbjct: 721 AGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ 757

BLAST of CsaV3_2G026160 vs. ExPASy TrEMBL
Match: A0A5A7V589 (Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00840 PE=3 SV=1)

HSP 1 Score: 1409.0 bits (3646), Expect = 0.0e+00
Identity = 704/764 (92.15%), Postives = 728/764 (95.29%), Query Frame = 0

Query: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLH---- 60
           MA RLYFWFSL PIFWLCPILTET NYIVHMNSAAMPKPFASRHSWYSATISS+LH    
Sbjct: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60

Query: 61  ---SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120
              SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH
Sbjct: 61  SSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120

Query: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTH 180
           SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESF DDGMSEIPS+WKGECESSTH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 180

Query: 181 FNVSFCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFG 240
           FNVSFCNNKLIGARFFNKGLI+  P ATIS+NSTRDTIGHGTHTST AAGSY+KEASFFG
Sbjct: 181 FNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFG 240

Query: 241 YGRGTARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDD 300
           YGRGTARGVAPRARVAIYKAIW+EGNSVSDVVAAIDQAISDGVDVISLSIGIDG+PLY+D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYND 300

Query: 301 PVAIATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVS 360
           PV+IATFAAVERGIFVATSAGNNGPQL TVHNGAPW+LNVAAGTMDRDFGGTITLSNGVS
Sbjct: 301 PVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 360

Query: 361 VLGSSLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTA 420
           VLGSSLFPLN T GLSPLPIVFMGGCQNLKKL+R GYKIVVCED+DGYSLTSQVDNVQTA
Sbjct: 361 VLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420

Query: 421 NVALGIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKP 480
            V LGIFISNIFDWDNLIQTPFPSIF+NPYHGNIIKDYIHKSSDPKAEV FHKTIL TKP
Sbjct: 421 KVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKP 480

Query: 481 APMVARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTS 540
           AP VARYSSRGPSQSCPFVLKPDIMAPGD ILASWPQN+PAMDVNSTPIY+KFNVISGTS
Sbjct: 481 APTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTS 540

Query: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGS 600
           MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTAD++DNTQT IKD GNNNKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGS 600

Query: 601 GHVNPNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660
           GHVNPNKA+DPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI
Sbjct: 601 GHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660

Query: 661 MIVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQK 720
           +I NSSDSKT KRKI GEFKRTLTKIGE+RATYEAKL GMKGFKVRVKP  L FKRKNQK
Sbjct: 661 IIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQK 720

Query: 721 LSFELKIAGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ 758
           LSFELKIAGSA ESN+VFGYLSWAEVGG HI+QSPIVV+GMR+Q
Sbjct: 721 LSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 764

BLAST of CsaV3_2G026160 vs. ExPASy TrEMBL
Match: A0A1S3BBC3 (subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=1)

HSP 1 Score: 1409.0 bits (3646), Expect = 0.0e+00
Identity = 704/764 (92.15%), Postives = 728/764 (95.29%), Query Frame = 0

Query: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLH---- 60
           MA RLYFWFSL PIFWLCPILTET NYIVHMNSAAMPKPFASRHSWYSATISS+LH    
Sbjct: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60

Query: 61  ---SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120
              SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH
Sbjct: 61  SSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120

Query: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTH 180
           SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESF DDGMSEIPS+WKGECESSTH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 180

Query: 181 FNVSFCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFG 240
           FNVSFCNNKLIGARFFNKGLI+  P ATIS+NSTRDTIGHGTHTST AAGSY+KEASFFG
Sbjct: 181 FNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFG 240

Query: 241 YGRGTARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDD 300
           YGRGTARGVAPRARVAIYKAIW+EGNSVSDVVAAIDQAISDGVDVISLSIGIDG+PLY+D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYND 300

Query: 301 PVAIATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVS 360
           PV+IATFAAVERGIFVATSAGNNGPQL TVHNGAPW+LNVAAGTMDRDFGGTITLSNGVS
Sbjct: 301 PVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 360

Query: 361 VLGSSLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTA 420
           VLGSSLFPLN T GLSPLPIVFMGGCQNLKKL+R GYKIVVCED+DGYSLTSQVDNVQTA
Sbjct: 361 VLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420

Query: 421 NVALGIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKP 480
            V LGIFISNIFDWDNLIQTPFPSIF+NPYHGNIIKDYIHKSSDPKAEV FHKTIL TKP
Sbjct: 421 KVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKP 480

Query: 481 APMVARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTS 540
           AP VARYSSRGPSQSCPFVLKPDIMAPGD ILASWPQN+PAMDVNSTPIY+KFNVISGTS
Sbjct: 481 APTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTS 540

Query: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGS 600
           MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTAD++DNTQT IKD GNNNKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGS 600

Query: 601 GHVNPNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660
           GHVNPNKA+DPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI
Sbjct: 601 GHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660

Query: 661 MIVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQK 720
           +I NSSDSKT KRKI GEFKRTLTKIGE+RATYEAKL GMKGFKVRVKP  L FKRKNQK
Sbjct: 661 IIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQK 720

Query: 721 LSFELKIAGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ 758
           LSFELKIAGSA ESN+VFGYLSWAEVGG HI+QSPIVV+GMR+Q
Sbjct: 721 LSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 764

BLAST of CsaV3_2G026160 vs. ExPASy TrEMBL
Match: A0A6J1HH07 (subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE=3 SV=1)

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 589/759 (77.60%), Postives = 657/759 (86.56%), Query Frame = 0

Query: 4   RLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLL-------H 63
           R++FW SL+ IF     LT+T NYIVHM+ AAMPKPFA+ HSWYSAT+SS+L        
Sbjct: 6   RMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65

Query: 64  SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSH 123
           ++SSSSSS PSKLIH Y HAI+GF ASLTPSQL+ALKNSPGY+SS+ DSSV  DTTHSS+
Sbjct: 66  TTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSN 125

Query: 124 FLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNV 183
           FL LS N GLLPIS YGSDVIIGFVDTG+WP+SESF DDG+S+IPS+WKGECES THFN 
Sbjct: 126 FLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNA 185

Query: 184 SFCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGR 243
           S CN KLIG RFFNKGLIS  P  TIS+NSTRDT GHGTHTSTTAAGSY+KEASFFGYG+
Sbjct: 186 SLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQ 245

Query: 244 GTARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVA 303
           GTARGVAPRARVAIYKAIW+EGN+VSDV+AAIDQAISDGVDVISLS+G+DGVPLY+DPVA
Sbjct: 246 GTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVA 305

Query: 304 IATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLG 363
           IATFAA+ERGIFVATSAGN GPQ  TVH+GAPW+LNVAAGTMDRDFGGTITL+NGVSVLG
Sbjct: 306 IATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLG 365

Query: 364 SSLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVA 423
           SSLFPLN    LSPLPIVFMG C NLKKL+R G+KIVVCEDSD YSL  QVDNV++A +A
Sbjct: 366 SSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIA 425

Query: 424 LGIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPM 483
           +G+FISNI DWDNLIQT FPSIFLN YHGN+IKDYI +SS+PKA V FHKTI+ TKPAP 
Sbjct: 426 VGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPS 485

Query: 484 VARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSC 543
           VARYSSRGPS+SCPFVLKPDIMAPGD ILASWPQNV A DV S PIYSKFNV+SGTSM+C
Sbjct: 486 VARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMAC 545

Query: 544 PHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHV 603
           PHAAGVAALLKGAHP WSPAAIRSAMMTTADI+DNTQT IKD GN NK ATPLAMGSGHV
Sbjct: 546 PHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHV 605

Query: 604 NPNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIV 663
           NPNKAIDP LIYD+GI+DY N+LCALNYT+NQI+ ITRS SN+CE   LDLNYPSFIM V
Sbjct: 606 NPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMTV 665

Query: 664 NSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSF 723
           N+SDS+T + ++S EFKR +T IGE  ATY AK+T MKG  V V+PNKL FKRKNQ LSF
Sbjct: 666 NASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSF 725

Query: 724 ELKIAGSA---RESNIVFGYLSWAEVGGGHIIQSPIVVS 753
           +LKI G     RES++VFGYL+W EVGGGH +QSPIVV+
Sbjct: 726 KLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVA 764

BLAST of CsaV3_2G026160 vs. ExPASy TrEMBL
Match: A0A6J1K9W2 (subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3 SV=1)

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 582/761 (76.48%), Postives = 654/761 (85.94%), Query Frame = 0

Query: 4   RLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSL------LHS 63
           R++F  SL+ I      LTET NYIVHM+ AAMPKPFA+ HSWYSAT+SS+      L +
Sbjct: 6   RMFFCLSLMVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65

Query: 64  SSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHF 123
           ++SSSSS PSKLIH Y HAI+GF A+LTPSQL+ALKNSPGY+SSV DSSV  DTTHSS+F
Sbjct: 66  TTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNF 125

Query: 124 LGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVS 183
           L L+ N GLLPIS YGSDVIIGFVDTG+WP+SESF D+ +S+IPS+WKGECES THFN S
Sbjct: 126 LALTPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESGTHFNAS 185

Query: 184 FCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRG 243
            CN KLIG RFFNKGLI+  P  TIS+NSTRDT GHGTHTSTTAAGSY+KEASFFGYG+G
Sbjct: 186 LCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQG 245

Query: 244 TARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAI 303
           TARGVAPRARVAIYKAIW+EGN VSDV+AAIDQAISDGVDVISLS+G+DGVPLY+DPVAI
Sbjct: 246 TARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAI 305

Query: 304 ATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGS 363
           ATFAA+ERGIFVATSAGN GPQ  TVH+GAPW+LNVAAGTMDRDFGGTITL+NGVSVLGS
Sbjct: 306 ATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGS 365

Query: 364 SLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVAL 423
           SLF LN    LSPLPIVFMG C NLKKL+R G+KIVVCEDSD YSL  QVDNVQ+A +A+
Sbjct: 366 SLFVLNSAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAV 425

Query: 424 GIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMV 483
           G+FISNI DWDNLIQT FPSIFLN YHGN+IKDYI +SS+PKA V FHKTI+ TKPAP V
Sbjct: 426 GVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGTKPAPSV 485

Query: 484 ARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCP 543
           ARYSSRGPS+SCP VLKPDIMAPGD ILASWPQ V A DV S PIYSKFNV+SGTSM+CP
Sbjct: 486 ARYSSRGPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSGTSMACP 545

Query: 544 HAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVN 603
           HAAGVAALLKGAHP WSPAAIRSAMMTTAD++DNTQT IKD GN NK ATPLAMGSGHVN
Sbjct: 546 HAAGVAALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAMGSGHVN 605

Query: 604 PNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVN 663
           PNKAIDP LIYD+GI+DY N+LCALNYT+NQI+ ITRS SN+CE PSLDLNYPSFI+ VN
Sbjct: 606 PNKAIDPGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPSFIITVN 665

Query: 664 SSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFE 723
           +SDS++ +R+IS EFKR +T IGE  ATY AK+T MKG  V V+P  L FKRKNQ LSF+
Sbjct: 666 ASDSESGRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKNQNLSFK 725

Query: 724 LKIAGSA---RESNIVFGYLSWAEVGGGHIIQSPIVVSGMR 756
           LKI G     RES++VFGYL+W EVGGGH +QSPIVV+G+R
Sbjct: 726 LKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAGLR 766

BLAST of CsaV3_2G026160 vs. TAIR 10
Match: AT5G67090.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 580.1 bits (1494), Expect = 2.6e-165
Identity = 321/745 (43.09%), Postives = 462/745 (62.01%), Query Frame = 0

Query: 11  LIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSSSSSSFPSKLI 70
           L+  F++  +  ET  YI+HM+ +A P PF+   SW+S T++S++       ++   K+I
Sbjct: 9   LVFSFFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVI-------TNRKPKII 68

Query: 71  HTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPIS 130
           + Y  ++ GF A LT S+L+ LK+ PGY+S   D  V + TT S  F+GL+S  G  P+S
Sbjct: 69  YAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTWPVS 128

Query: 131 KYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFN 190
            YG+ ++IG +DTGIWPDS SF DDG+  +PSKWKG CE ++    S CN KLIGA+ FN
Sbjct: 129 NYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFNSS---SLCNKKLIGAKVFN 188

Query: 191 KGLISGLPKATIS----INSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRA 250
           KGL +  P    +     +S  DTIGHGTH +  AAG+++K AS+F Y +GTA G+AP A
Sbjct: 189 KGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIAPHA 248

Query: 251 RVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGI--------DGVPLYDDPVAIA 310
            +AIYKA WEEG   SDV+AAIDQAI DGV VISLS+G+        DG  L +DP+A+A
Sbjct: 249 HLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPIAVA 308

Query: 311 TFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSS 370
           +FAA+++G+FV TS GN+GP   ++ NGAPW++ V AGT+ R F GT+T  N VS    S
Sbjct: 309 SFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFSFPS 368

Query: 371 LFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALG 430
           LFP    +   P+  +  G  +N    +    +IVVC  ++  ++ S++  +++   A  
Sbjct: 369 LFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIRSTGAAAV 428

Query: 431 IFISN-IFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKS-SDPKAEVTFHKTILRTKPAPM 490
           + I++ + +  + I+  FP  F+   H   I+ Y   + ++  A++ F KT++ TKPAP 
Sbjct: 429 VLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKPAPE 488

Query: 491 VARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSC 550
           V  YSSRGP  S P +LKPDI+APG  IL++WP         + P++S FN+++GTSM+ 
Sbjct: 489 VGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTSMAA 548

Query: 551 PHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHV 610
           PH AGVAAL+K  HP WSP+AI+SA+MTTA  LDN                PLA+G+GHV
Sbjct: 549 PHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGAGHV 608

Query: 611 NPNKAIDPDLIYDVGIQDYVNVLC-ALNYTENQIRIITRSD-SNNCENPSLDLNYPSFIM 670
           + NK ++P LIYD   QD++N LC     +   I IITRS+ S+ C+ PS  LNYPS I 
Sbjct: 609 STNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPSIIA 668

Query: 671 IVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKL 730
              S  S  +       FKRTLT +GE + +Y  ++ G+KG  V V+P KL F  KN+KL
Sbjct: 669 YFTSDQSSPK------IFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNEKL 715

Query: 731 SFELKI-AGSARESNIVFGYLSWAE 739
           S+ +++ +    + N+V+G +SW +
Sbjct: 729 SYTVRLESPRGLQENVVYGLVSWVD 715

BLAST of CsaV3_2G026160 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.6e-146
Identity = 312/764 (40.84%), Postives = 442/764 (57.85%), Query Frame = 0

Query: 27  YIVHMNSAAMPKPFASRHSWYSATISSLLHSSSSSSSSFPSKLIHTYNHAISGFCASLTP 86
           YIVH++  A P  F +   WY+++++SL        +S P  +IHTY+    GF A LT 
Sbjct: 28  YIVHVDHEAKPSIFPTHFHWYTSSLASL--------TSSPPSIIHTYDTVFHGFSARLTS 87

Query: 87  SQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNH--GLLPISKYGSDVIIGFVDTG 146
                L + P  +S + +   H+ TT S  FLGL S    GLL  S +GSD++IG +DTG
Sbjct: 88  QDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTG 147

Query: 147 IWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFNKG--LISGLPKATI 206
           +WP+  SF D G+  +P KWKG+C +S  F  S CN KL+GARFF  G    +G    T 
Sbjct: 148 VWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETT 207

Query: 207 SINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSVS 266
              S RD+ GHGTHT++ +AG Y+  AS  GY  G A G+AP+AR+A YK  W  G   S
Sbjct: 208 EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDS 267

Query: 267 DVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATFAAVERGIFVATSAGNNGPQLET 326
           D++AA D A++DGVDVISLS+G   VP Y D +AI  F A++RGIFV+ SAGN GP   T
Sbjct: 268 DILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALT 327

Query: 327 VHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNITTGLSP---LPIVF---- 386
           V N APW+  V AGT+DRDF   + L NG  + G S++      GL P    P+V+    
Sbjct: 328 VTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYG---GPGLDPGRMYPLVYGGSL 387

Query: 387 MGGCQNLKKLRRTGY--------KIVVCEDSDGYSLTSQVDNVQTANVALGIFISN-IFD 446
           +GG      L   G         KIV+C+   G +  +    +   N  LG+ I+N +FD
Sbjct: 388 LGGDGYSSSLCLEGSLDPNLVKGKIVLCD--RGINSRATKGEIVRKNGGLGMIIANGVFD 447

Query: 447 WDNLIQT--PFPSIFLNPYHGNIIKDYIHKSS------DPKAEVTFHKTILRTKPAPMVA 506
            + L+      P+  +    G+ I+ YI +SS       P A + F  T L  +PAP+VA
Sbjct: 448 GEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVA 507

Query: 507 RYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPH 566
            +S+RGP+   P +LKPD++APG  ILA+WP  +    V S    ++FN++SGTSM+CPH
Sbjct: 508 SFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPH 567

Query: 567 AAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNP 626
            +G+AALLK AHP WSPAAIRSA++TTA  +DN+   + D    N  ++ +  GSGHV+P
Sbjct: 568 VSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT-SSVMDYGSGHVHP 627

Query: 627 NKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSL-----DLNYPSFI 686
            KA+DP L+YD+   DY+N LC  NYT   I  ITR  + +C+         +LNYPSF 
Sbjct: 628 TKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA-DCDGARRAGHVGNLNYPSFS 687

Query: 687 MIVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQK 746
           ++      +  + K+S  F RT+T +G+  + YE K+   +G  V V+P KL+F+R  QK
Sbjct: 688 VVF----QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQK 747

Query: 747 LSFELKIAG-----SARESNIVFGYLSWAEVGGGHIIQSPIVVS 753
           LSF +++       S   +N+  G++ W++  G   + SP+VV+
Sbjct: 748 LSFVVRVKTTEVKLSPGATNVETGHIVWSD--GKRNVTSPLVVT 770

BLAST of CsaV3_2G026160 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 516.2 bits (1328), Expect = 4.6e-146
Identity = 298/756 (39.42%), Postives = 436/756 (57.67%), Query Frame = 0

Query: 22  TETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSSSSSSFPSKLIHTYNHAISGFC 81
           T  + YI+ +N +  P+ F + H WY++ ++S             S L++TY  +  GF 
Sbjct: 25  TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS------------ESSLLYTYTTSFHGFS 84

Query: 82  ASLTPSQLEALKNSPGYLSSVLDSSVH-VDTTHSSHFLGLSSNHGLLPISKYGSDVIIGF 141
           A L  ++ ++L +S   +  + +  ++ + TT +  FLGL+S  G+  +    + VIIG 
Sbjct: 85  AYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGV 144

Query: 142 VDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFNKGLI----SG 201
           +DTG+WP+S SF D  M EIPSKWKGECES + F+   CN KLIGAR F+KG       G
Sbjct: 145 LDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGG 204

Query: 202 LPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRARVAIYKAIWE 261
                 S+ S RD  GHGTHTSTTAAGS ++ ASF GY  GTARG+A RARVA YK  W 
Sbjct: 205 FSSKRESV-SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWS 264

Query: 262 EGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATFAAVERGIFVATSAGNN 321
            G   SD++AA+D+AI DGVDV+SLS+G    P Y D +AI  F+A+ERG+FV+ SAGN+
Sbjct: 265 TGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNS 324

Query: 322 GPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNITTGLSPLPIVFM 381
           GP   +V N APW++ V AGT+DRDF     L NG  + G SL+   +  G  PL +V+ 
Sbjct: 325 GPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKPLELVYN 384

Query: 382 GGCQNLKKLRRTGY--------KIVVCEDSDGYSLTSQVDNVQTANVALGIFISN-IFDW 441
            G  +   L   G         KIVVC+   G +   +   V      LG+ ++N     
Sbjct: 385 KGNSSSSNLCLPGSLDSSIVRGKIVVCD--RGVNARVEKGAVVRDAGGLGMIMANTAASG 444

Query: 442 DNLIQTP--FPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARYSSRGP 501
           + L+      P+I +    G+++++Y+   S P A + F  T+L  KP+P+VA +SSRGP
Sbjct: 445 EELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGP 504

Query: 502 SQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAAGVAAL 561
           +   P +LKPD++ PG  ILA W   +    ++     ++FN++SGTSMSCPH +G+A L
Sbjct: 505 NTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGL 564

Query: 562 LKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNKAIDPD 621
           LK AHP+WSP+AI+SA+MTTA +LDNT   + D  +N+  + P A GSGHV+P KA+ P 
Sbjct: 565 LKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNS-LSNPYAHGSGHVDPQKALSPG 624

Query: 622 LIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLD---LNYPSFIMIVNSSDSK 681
           L+YD+  ++Y+  LC+L+YT + I  I +  S NC     D   LNYPSF ++       
Sbjct: 625 LVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGG---- 684

Query: 682 TRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKIA- 741
             KR +   + R +T +G   + Y+  + G     + VKP+KL+FK   +K  + +    
Sbjct: 685 --KRVV--RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 744

Query: 742 --GSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMR 756
             G +  +   FG ++W+     H ++SP+  S  R
Sbjct: 745 KKGVSMTNKAEFGSITWS--NPQHEVRSPVAFSWNR 753

BLAST of CsaV3_2G026160 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 496.1 bits (1276), Expect = 4.9e-140
Identity = 304/748 (40.64%), Postives = 429/748 (57.35%), Query Frame = 0

Query: 27  YIVHMNSAAMPKPFASRHSWYSATISSLLHSSSSSSSSFPSKLIHTYNHAISGFCASLTP 86
           YIVHM  + MP  F    +WY +++ S+  S         ++L++TY +AI GF   LT 
Sbjct: 32  YIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---------AELLYTYENAIHGFSTRLTQ 91

Query: 87  SQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHG-LLPISKYGSDVIIGFVDTGI 146
            + ++L   PG +S + +    + TT +  FLGL  +   L P +   SDV++G +DTG+
Sbjct: 92  EEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGV 151

Query: 147 WPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFNKGLIS--GLPKATIS 206
           WP+S+S+ D+G   IPS WKG CE+ T+F  S CN KLIGARFF +G  S  G    +  
Sbjct: 152 WPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKE 211

Query: 207 INSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSVSD 266
             S RD  GHGTHTS+TAAGS ++ AS  GY  GTARG+APRARVA+YK  W  G   SD
Sbjct: 212 SRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSD 271

Query: 267 VVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATFAAVERGIFVATSAGNNGPQLETV 326
           ++AAID+AI+D V+V+S+S+G      Y D VAI  FAA+ERGI V+ SAGN GP   ++
Sbjct: 272 ILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSL 331

Query: 327 HNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNITTGLSPLPIVFMGGCQNLK 386
            N APW+  V AGT+DRDF     L NG +  G SLF          LP ++ G   N  
Sbjct: 332 SNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD-KLLPFIYAGNASNAT 391

Query: 387 --KLRRTGY--------KIVVCEDSDGYSLTSQVDNVQTANVALGIFISN-IFDWDNLIQ 446
              L  TG         KIV+C+   G +   Q  +V  A   +G+ ++N   + + L+ 
Sbjct: 392 NGNLCMTGTLIPEKVKGKIVMCD--RGINARVQKGDVVKAAGGVGMILANTAANGEELVA 451

Query: 447 TP--FPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARYSSRGPSQSCP 506
                P+  +    G+II+ Y+    +P A ++   T++  KP+P+VA +SSRGP+   P
Sbjct: 452 DAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITP 511

Query: 507 FVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAAGVAALLKGAH 566
            +LKPD++APG  ILA+W        + S     +FN+ISGTSMSCPH +G+AALLK  H
Sbjct: 512 NILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVH 571

Query: 567 PQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNKAIDPDLIYDV 626
           P+WSPAAIRSA+MTTA         + D     K +TP   G+GHV+P  A +P LIYD+
Sbjct: 572 PEWSPAAIRSALMTTAYKTYKDGKPLLDIA-TGKPSTPFDHGAGHVSPTTATNPGLIYDL 631

Query: 627 GIQDYVNVLCALNYTENQIRIITRSDSNNCENPS---LDLNYPSFIMIVNSSDSKTRKRK 686
             +DY+  LCALNYT  QIR ++R +     + S    DLNYPSF + V+   +      
Sbjct: 632 TTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAY----- 691

Query: 687 ISGEFKRTLTKIGEHRATYEAKLTG-MKGFKVRVKPNKLNFKRKNQKLSFEL--KIAGSA 746
              ++ RT+T +G    TY  K+T    G K+ V+P  LNFK  N+K S+ +   +  S 
Sbjct: 692 ---KYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSK 751

Query: 747 RESNIVFGYLSWAEVGGGHIIQSPIVVS 753
              +  FG + W++  G H++ SP+ +S
Sbjct: 752 PSGSNSFGSIEWSD--GKHVVGSPVAIS 755

BLAST of CsaV3_2G026160 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 491.9 bits (1265), Expect = 9.3e-139
Identity = 295/760 (38.82%), Postives = 430/760 (56.58%), Query Frame = 0

Query: 25  RNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSSSSSSFPSKLIHTYNHAISGFCASL 84
           + +I  ++  +MP  F + + WYS   +              S+++H Y+    GF A +
Sbjct: 26  KTFIFRIDGGSMPSIFPTHYHWYSTEFAE------------ESRIVHVYHTVFHGFSAVV 85

Query: 85  TPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTG 144
           TP + + L+N P  L+   D    + TT S  FLGL +  GL   S YGSDVIIG  DTG
Sbjct: 86  TPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIGVFDTG 145

Query: 145 IWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFNKG----LISGLPKA 204
           IWP+  SF D  +  IP +W+G CES   F+   CN K+IGARFF KG    +I G+ K 
Sbjct: 146 IWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGINK- 205

Query: 205 TISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNS 264
           T+   S RD  GHGTHTS+TAAG +  +AS  GY  G A+GVAP+AR+A YK  W++   
Sbjct: 206 TVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGC 265

Query: 265 V-SDVVAAIDQAISDGVDVISLSI-GIDGV--PLYDDPVAIATFAAVERGIFVATSAGNN 324
           + SD++AA D A+ DGVDVIS+SI G DG+  P Y DP+AI ++ A  +GIFV++SAGN 
Sbjct: 266 LDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNE 325

Query: 325 GPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLF---PLNITTGLSPLPI 384
           GP   +V N APW+  V A T+DR+F     L +G  + G SL+   PLN        P+
Sbjct: 326 GPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRM----FPV 385

Query: 385 VFMGG---------CQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIFISNI 444
           V+ G           +N    ++   KIV+C+      +   +   +   V + I  +  
Sbjct: 386 VYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGM-ILANGA 445

Query: 445 FDWDNLIQTP--FPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARYSS 504
            + + L+      P+  +    G+ IK Y     +P A + F  TI+  KPAP++A +S 
Sbjct: 446 SNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSG 505

Query: 505 RGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAAGV 564
           RGP+   P +LKPD++APG  ILA+W   V    + S P  ++FN++SGTSM+CPH +G 
Sbjct: 506 RGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGA 565

Query: 565 AALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNKAI 624
           AALLK AHP WSPA IRSAMMTT +++DN+   + D  +  K ATP   GSGH+N  +A+
Sbjct: 566 AALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGHLNLGRAM 625

Query: 625 DPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSN--NCENPSL-DLNYPSFIMIVNSS 684
           +P L+YD+   DY+  LC++ Y    I++ITR+         PS  +LNYPS   +  ++
Sbjct: 626 NPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTN 685

Query: 685 DSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELK 744
               R+  +S    RT T +G+  A Y A++   +G  V VKP +L F    ++ S+ + 
Sbjct: 686 ----RRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVT 745

Query: 745 IAGSAR-----ESNIVFGYLSWAEVGGGHIIQSPIVVSGM 755
           +  + R     E+  VFG ++W + GG H+++SPIVV+ M
Sbjct: 746 VTVNTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVVTQM 761