CsaV3_2G010790 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_2G010790
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptioncytochrome P450 704B1
Locationchr2: 7968319 .. 7971807 (-)
RNA-Seq ExpressionCsaV3_2G010790
SyntenyCsaV3_2G010790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAAGAGGTGGCATAATAGAGTTTGTTTTGGAAGCTATTGGTAAAATTAAGAAATGGGAATTAGTTTATTGGAGTGGTGTAATAATAATAATAGAGCTGATTCAGAAGAAGAATGGTACATGAGATCAAGTAATTTAAGAAGAAGTGGAGTAGTAGCGTTGGTGTTGGTGATGATAGCAATAATAAGTGCATGTTGGATAATTATTGTTCATAGATGGAACCAAAGGAAGAAGATGAGGAGAGGTCCAAAAACATGGCCAATCGTAGGAGCAGCAATAGAACAGTTTGTAAATTACCACAGAATGCATGATTGGATTTTGGGTTACCTTAATCTCTACAGAACTGTTGTGGTTCCCATGCCTTTCACTACTTACACTTATATTGCTGACCCTGCCAATGTTCAACATATTCTCAAGACCAACTTTGCTAATTATCCAAAGGTACAAGTACAATTGCCTTACATTCATCTTGTTAATTTACCTTCTTTTTTTTTTTCTTTCTTTTTAAAGAAAAAGTATGAGTAGTAATTTAGTTAATATTATTGTATTAGGGTGATGTATATCATTCATACATGGAAGTGCTTCTTGGAGATGGGATCTTCAACGTAGATGGAGAGTTGTGGAGGAAACAAAGGAAAACTGCTAGCTTCGAATTTGCATCTAAGAATTTGAGGGATTTCAGTACCTTAGTTTTTAGAGACTATGGTCTCAAGCTATCAACTATTTTAACACGTGCCTCTTTACTCAACCAACAAATTGACATGCAGGTGACTTTGTTCATCAAACCCAACCTTTTATTTATTTGTTTTTATTACAGTAAATAAATATTATTCATTTTTAAACGTAGGAATTGTTGATGAGGATGACTTTAGATTCCATATGCAAGGTTGGCTTTGGTGTTGAAATTGGATCATTGGCTCCCAATTTGCCAGAAAATAAGTTTGCTAAGGCCTTTGATACAGCCAATATGATCGTTACCCTTCGCTTCATTGATCCTCTGTGGAAAATTAAAAGATTTCTGAATCTGGGATCTGAAGCTCTTCTTGATAAATCCATCAAAACCATTGATGATTTCACTTATTCTGTCATTGCCACAAGGAAAAAAGAGATCCAAGAAGCTCAAACTACACACACAGATCCCAATAAGGTCATTTACCGTAATACTCTCTACTTCATTGCCCCCTTCATTTTATTAACTTCATTTTTCGTCTATTTTAATATTTTAACTTTGGAAAATTTTGCAAAACTAAAAATTCTTCTTTCAGTTTCACCTTTCATAAATAGCATCCACTGAATTTTGTATAAAAAGTTTACCTTTTCGACTTATATCTTGACCATTATTGATTCTCTTGAATTTTTTGGGTGATCTTTTTTATCTATCCAAAATTAAGAATCACACACGGCCAAATATTACGATAAATTTGGAAAAATGGCATAATGTTTGAAAAAAATATTGACATGGATCTCAATTCATCCTCCACACAAAATTGTACTAAAAAAAATCCAAAACAATTTATTATTTTGAGCTTTTTTGGTATAATTGTCTTCACAGTGAACACCAACATTAGACTGTCTTCGTAAAATTAAAAAAGGATTTTGGATTAATTTAAGTATATAGAACAAAACTGACCAATAACAAAATAAGCGTGTAAGGGTTGAGTTAATTTTTTTTCATAAATTCAGATTTATCATGTTAGAATTTATTATCTCCTTTTACATCATTTATTACTTGTTCGTCATCTTCTCATTTTCCATCGGTGTCATCTTTTTTATAAAGACAATATTTTCTTCTTGTTTCTTTTTTACTTTATCAAGTATTGTGTCCTTATTTTTTTCTTTTTTTGTCACTACATGATTAGTACAAGATTGTGTATTAAGATCGTTTAGATTGAGTACAATATCGGTTAGAATGATTACAATATTATTTAGTGTAGGTACTGCAACCAATTAATTACTGATATTTTTGTTATTTCACGTTGTGAGCTTGTGTGTTTTTTTCATTTTTAAAATTGTTTTATACGTTGTAAATATTGATGCGTTTTGTTTTTTTTAAAAAAAAAAACTCATTAATTATGATAATTTTTTCTCAACTTTCAACTTCAAATAAATCTTTTAACTTTTAAAAATGTAGAAAATACTAAAAGAAAAATTAAATTTGTCTATTATTATTTTTCAGTTTTTAAAGAAAGATATTATTTTGTAATAAAAAAATTGAAGCTTATATAAGGTGGAATTCAATTAATATATGTTTTTGGTATGAGACGATCACAGATGAAACATGACATATTGTCGAGATTTATTGAGTTAGGGGAAGACCCAAACAACAAATTCACAGACAAAAGCTTAAGAGATATTGTTCTGAACTTCGTGATTGCCGGACGTGACACAACAGCCGCAACTCTTTCATGGAGTATTTATATGCTAATGACTCATTCAGATGTCTCCCAAAAACTTTACTCAGAGCTCAAAAGTTTTGAAGAAGAAAGAGCCAAAGAACACTCCATTTCATTGCTTCAATATCAACCAAATGATATTCAATCCTTTGACACAAGAGTTGTACAATTTTCACAACTTCTTAATTATGATTCTCTTGCTCGATTACATTACTTGCATGCTATCGTTACTGAGACACTTCGTCTTTACCCTGCAGTTCCCCAGGTACGAAATTATACATTACTACTCCTTAGATAAACCTATGTTAAACTCGGTGGCAAACTTGCAAATACCATAATCAAACCTTAATGCAAAAGATAATCTAATAGATTTTGTTAAATATGTAATTATTTAAAATGTTGTTATACCCTTGAGGTATTAATTAATTTTAAAAGTGTTATCCATGCATTGCAATTATCCGATAGGACCCAAAGGGAATTTTGGAGGATGATGTATTGCCAGATGGGACAAAGGTAAGAGCTGGAGGAATGGTGACATATGTTCCTTACTCGATGGGAAGAATGGAATACAATTGGGGTTATGATGCAACTTCTTTTAAACCTGAAAGATGGCTCAAACAAGGCATTTTCCAGAATGAATCTCCATTCAAGTTCACAGCTTTTCAGGTCACTTTCCTTTTGATCTTCACTTATATGTTATCACTGAAACTATAAGAAGAGAATCGATCTGTTTCATTTAACACTTCAAGTATTTTGTTTTAAAATAATAATGTTATTATTCAAAATAAATAACTTTTTTTTCTCTAGTCAATTAGTAGACATTTAAGTCGTTTAATTAATTAGAGATTTTGAAATGTTATTGTAGGCTGGACCAAGGATCTGCATGGGGAAAGACTCGGCATATCTACAAATGAAAATGACTCTTGCCATTTTATGCAGATTCTACAAATTTAGTTTGGTGAGTGGGCATAGAGTCGACTACAGAATGATGACTATTCTCTCAATGGCTAATGGCTTGAAAGTTACTGTGGCTCCACGCTCTTAAAAATTTTATCTCTTTTTATTTACACTTTATCATCAACAATCAAAACCAAAA

mRNA sequence

ATGGGAATTAGTTTATTGGAGTGGTGTAATAATAATAATAGAGCTGATTCAGAAGAAGAATGGTACATGAGATCAAGTAATTTAAGAAGAAGTGGAGTAGTAGCGTTGGTGTTGGTGATGATAGCAATAATAAGTGCATGTTGGATAATTATTGTTCATAGATGGAACCAAAGGAAGAAGATGAGGAGAGGTCCAAAAACATGGCCAATCGTAGGAGCAGCAATAGAACAGTTTGTAAATTACCACAGAATGCATGATTGGATTTTGGGTTACCTTAATCTCTACAGAACTGTTGTGGTTCCCATGCCTTTCACTACTTACACTTATATTGCTGACCCTGCCAATGTTCAACATATTCTCAAGACCAACTTTGCTAATTATCCAAAGGGTGATGTATATCATTCATACATGGAAGTGCTTCTTGGAGATGGGATCTTCAACGTAGATGGAGAGTTGTGGAGGAAACAAAGGAAAACTGCTAGCTTCGAATTTGCATCTAAGAATTTGAGGGATTTCAGTACCTTAGTTTTTAGAGACTATGGTCTCAAGCTATCAACTATTTTAACACGTGCCTCTTTACTCAACCAACAAATTGACATGCAGGAATTGTTGATGAGGATGACTTTAGATTCCATATGCAAGGTTGGCTTTGGTGTTGAAATTGGATCATTGGCTCCCAATTTGCCAGAAAATAAGTTTGCTAAGGCCTTTGATACAGCCAATATGATCGTTACCCTTCGCTTCATTGATCCTCTGTGGAAAATTAAAAGATTTCTGAATCTGGGATCTGAAGCTCTTCTTGATAAATCCATCAAAACCATTGATGATTTCACTTATTCTGTCATTGCCACAAGGAAAAAAGAGATCCAAGAAGCTCAAACTACACACACAGATCCCAATAAGATGAAACATGACATATTGTCGAGATTTATTGAGTTAGGGGAAGACCCAAACAACAAATTCACAGACAAAAGCTTAAGAGATATTGTTCTGAACTTCGTGATTGCCGGACGTGACACAACAGCCGCAACTCTTTCATGGAGTATTTATATGCTAATGACTCATTCAGATGTCTCCCAAAAACTTTACTCAGAGCTCAAAAGTTTTGAAGAAGAAAGAGCCAAAGAACACTCCATTTCATTGCTTCAATATCAACCAAATGATATTCAATCCTTTGACACAAGAGTTGTACAATTTTCACAACTTCTTAATTATGATTCTCTTGCTCGATTACATTACTTGCATGCTATCGTTACTGAGACACTTCGTCTTTACCCTGCAGTTCCCCAGGACCCAAAGGGAATTTTGGAGGATGATGTATTGCCAGATGGGACAAAGGTAAGAGCTGGAGGAATGGTGACATATGTTCCTTACTCGATGGGAAGAATGGAATACAATTGGGGTTATGATGCAACTTCTTTTAAACCTGAAAGATGGCTCAAACAAGGCATTTTCCAGAATGAATCTCCATTCAAGTTCACAGCTTTTCAGGCTGGACCAAGGATCTGCATGGGGAAAGACTCGGCATATCTACAAATGAAAATGACTCTTGCCATTTTATGCAGATTCTACAAATTTAGTTTGGTGAGTGGGCATAGAGTCGACTACAGAATGATGACTATTCTCTCAATGGCTAATGGCTTGAAAGTTACTGTGGCTCCACGCTCTTAA

Coding sequence (CDS)

ATGGGAATTAGTTTATTGGAGTGGTGTAATAATAATAATAGAGCTGATTCAGAAGAAGAATGGTACATGAGATCAAGTAATTTAAGAAGAAGTGGAGTAGTAGCGTTGGTGTTGGTGATGATAGCAATAATAAGTGCATGTTGGATAATTATTGTTCATAGATGGAACCAAAGGAAGAAGATGAGGAGAGGTCCAAAAACATGGCCAATCGTAGGAGCAGCAATAGAACAGTTTGTAAATTACCACAGAATGCATGATTGGATTTTGGGTTACCTTAATCTCTACAGAACTGTTGTGGTTCCCATGCCTTTCACTACTTACACTTATATTGCTGACCCTGCCAATGTTCAACATATTCTCAAGACCAACTTTGCTAATTATCCAAAGGGTGATGTATATCATTCATACATGGAAGTGCTTCTTGGAGATGGGATCTTCAACGTAGATGGAGAGTTGTGGAGGAAACAAAGGAAAACTGCTAGCTTCGAATTTGCATCTAAGAATTTGAGGGATTTCAGTACCTTAGTTTTTAGAGACTATGGTCTCAAGCTATCAACTATTTTAACACGTGCCTCTTTACTCAACCAACAAATTGACATGCAGGAATTGTTGATGAGGATGACTTTAGATTCCATATGCAAGGTTGGCTTTGGTGTTGAAATTGGATCATTGGCTCCCAATTTGCCAGAAAATAAGTTTGCTAAGGCCTTTGATACAGCCAATATGATCGTTACCCTTCGCTTCATTGATCCTCTGTGGAAAATTAAAAGATTTCTGAATCTGGGATCTGAAGCTCTTCTTGATAAATCCATCAAAACCATTGATGATTTCACTTATTCTGTCATTGCCACAAGGAAAAAAGAGATCCAAGAAGCTCAAACTACACACACAGATCCCAATAAGATGAAACATGACATATTGTCGAGATTTATTGAGTTAGGGGAAGACCCAAACAACAAATTCACAGACAAAAGCTTAAGAGATATTGTTCTGAACTTCGTGATTGCCGGACGTGACACAACAGCCGCAACTCTTTCATGGAGTATTTATATGCTAATGACTCATTCAGATGTCTCCCAAAAACTTTACTCAGAGCTCAAAAGTTTTGAAGAAGAAAGAGCCAAAGAACACTCCATTTCATTGCTTCAATATCAACCAAATGATATTCAATCCTTTGACACAAGAGTTGTACAATTTTCACAACTTCTTAATTATGATTCTCTTGCTCGATTACATTACTTGCATGCTATCGTTACTGAGACACTTCGTCTTTACCCTGCAGTTCCCCAGGACCCAAAGGGAATTTTGGAGGATGATGTATTGCCAGATGGGACAAAGGTAAGAGCTGGAGGAATGGTGACATATGTTCCTTACTCGATGGGAAGAATGGAATACAATTGGGGTTATGATGCAACTTCTTTTAAACCTGAAAGATGGCTCAAACAAGGCATTTTCCAGAATGAATCTCCATTCAAGTTCACAGCTTTTCAGGCTGGACCAAGGATCTGCATGGGGAAAGACTCGGCATATCTACAAATGAAAATGACTCTTGCCATTTTATGCAGATTCTACAAATTTAGTTTGGTGAGTGGGCATAGAGTCGACTACAGAATGATGACTATTCTCTCAATGGCTAATGGCTTGAAAGTTACTGTGGCTCCACGCTCTTAA

Protein sequence

MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPRS*
Homology
BLAST of CsaV3_2G010790 vs. NCBI nr
Match: XP_004138991.2 (cytochrome P450 704B1 [Cucumis sativus] >KGN61391.1 hypothetical protein Csa_006668 [Cucumis sativus])

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60
           MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK
Sbjct: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60

Query: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120
           MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL
Sbjct: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120

Query: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180
           KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY
Sbjct: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180

Query: 181 GLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA 240
           GLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA
Sbjct: 181 GLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA 240

Query: 241 NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPN 300
           NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPN
Sbjct: 241 NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPN 300

Query: 301 KMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQ 360
           KMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQ
Sbjct: 301 KMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQ 360

Query: 361 KLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTE 420
           KLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTE
Sbjct: 361 KLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTE 420

Query: 421 TLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWL 480
           TLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWL
Sbjct: 421 TLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWL 480

Query: 481 KQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMT 540
           KQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMT
Sbjct: 481 KQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMT 540

Query: 541 ILSMANGLKVTVAPRS 557
           ILSMANGLKVTVAPRS
Sbjct: 541 ILSMANGLKVTVAPRS 556

BLAST of CsaV3_2G010790 vs. NCBI nr
Match: XP_008441698.1 (PREDICTED: cytochrome P450 704B1 [Cucumis melo])

HSP 1 Score: 1061.2 bits (2743), Expect = 3.0e-306
Identity = 526/557 (94.43%), Postives = 542/557 (97.31%), Query Frame = 0

Query: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60
           MGISLLEWCNNNNRA+SEEEW+MRSSNLRRS VV LVL +I I++ CWIIIVHRWN+RKK
Sbjct: 1   MGISLLEWCNNNNRANSEEEWHMRSSNLRRSVVVVLVLGVITIVT-CWIIIVHRWNERKK 60

Query: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120
           MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLY+TVVVPMPFTTYTYIADPANVQHIL
Sbjct: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYKTVVVPMPFTTYTYIADPANVQHIL 120

Query: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180
           KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY
Sbjct: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180

Query: 181 GLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA 240
            LKLSTILTRASLLNQQ+DMQELL RMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA
Sbjct: 181 SLKLSTILTRASLLNQQLDMQELLTRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA 240

Query: 241 NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDP- 300
           NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIK IDDFTYSVIATRKKEIQEAQTT T+P 
Sbjct: 241 NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKIIDDFTYSVIATRKKEIQEAQTTPTEPN 300

Query: 301 NKMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVS 360
           NK+KHDILSRFIELGEDPNNKFTDKSLRD+VLNFVIAGRDTTAATLSWSIYMLMTHSDVS
Sbjct: 301 NKIKHDILSRFIELGEDPNNKFTDKSLRDVVLNFVIAGRDTTAATLSWSIYMLMTHSDVS 360

Query: 361 QKLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVT 420
           QKLYSELKSFEEERAKEHSISLLQYQPNDIQSF+ RV+QFSQLLNYDSLARLHYLHAI+T
Sbjct: 361 QKLYSELKSFEEERAKEHSISLLQYQPNDIQSFERRVIQFSQLLNYDSLARLHYLHAIIT 420

Query: 421 ETLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERW 480
           ETLRLYPAVPQDPKGILEDD+LPDGTKVRAGGMVTYVPYSMGRMEYNWG DATSF PERW
Sbjct: 421 ETLRLYPAVPQDPKGILEDDILPDGTKVRAGGMVTYVPYSMGRMEYNWGPDATSFNPERW 480

Query: 481 LKQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMM 540
           LKQGIF NESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRV+YRMM
Sbjct: 481 LKQGIFHNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVNYRMM 540

Query: 541 TILSMANGLKVTVAPRS 557
           TILSMANGLKVTVA RS
Sbjct: 541 TILSMANGLKVTVASRS 556

BLAST of CsaV3_2G010790 vs. NCBI nr
Match: KAA0032319.1 (cytochrome P450 704B1 [Cucumis melo var. makuwa] >TYJ99426.1 cytochrome P450 704B1 [Cucumis melo var. makuwa])

HSP 1 Score: 977.6 bits (2526), Expect = 4.4e-281
Identity = 482/504 (95.63%), Postives = 494/504 (98.02%), Query Frame = 0

Query: 54  RWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADP 113
           +WN+RKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLY+TVVVPMPFTTYTYIADP
Sbjct: 6   KWNERKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYKTVVVPMPFTTYTYIADP 65

Query: 114 ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS 173
           ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS
Sbjct: 66  ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS 125

Query: 174 TLVFRDYGLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKF 233
           TLVFRDY LKLSTILTRASLLNQQ+DMQELL RMTLDSICKVGFGVEIGSLAPNLPENKF
Sbjct: 126 TLVFRDYSLKLSTILTRASLLNQQLDMQELLTRMTLDSICKVGFGVEIGSLAPNLPENKF 185

Query: 234 AKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQ 293
           AKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIK IDDFTYSVIATRKKEIQEAQ
Sbjct: 186 AKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKIIDDFTYSVIATRKKEIQEAQ 245

Query: 294 TTHTDP-NKMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYML 353
           TT T+P NK+KHDILSRFIELGEDPNNKFTDKSLRD+VLNFVIAGRDTTAATLSWSIYML
Sbjct: 246 TTPTEPNNKIKHDILSRFIELGEDPNNKFTDKSLRDVVLNFVIAGRDTTAATLSWSIYML 305

Query: 354 MTHSDVSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLH 413
           MTHSDVSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSF+ RV+QFSQLLNYDSLARLH
Sbjct: 306 MTHSDVSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSFERRVIQFSQLLNYDSLARLH 365

Query: 414 YLHAIVTETLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDAT 473
           YLHAI+TETLRLYPAVPQDPKGILEDD+LPDGTKVRAGGMVTYVPYSMGRMEYNWG DAT
Sbjct: 366 YLHAIITETLRLYPAVPQDPKGILEDDILPDGTKVRAGGMVTYVPYSMGRMEYNWGPDAT 425

Query: 474 SFKPERWLKQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGH 533
           SF PERWLKQGIF NESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGH
Sbjct: 426 SFNPERWLKQGIFHNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGH 485

Query: 534 RVDYRMMTILSMANGLKVTVAPRS 557
           RV+YRMMTILSMANGLKVTVA RS
Sbjct: 486 RVNYRMMTILSMANGLKVTVASRS 509

BLAST of CsaV3_2G010790 vs. NCBI nr
Match: XP_022946229.1 (cytochrome P450 704B1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 900.2 bits (2325), Expect = 8.9e-258
Identity = 446/540 (82.59%), Postives = 483/540 (89.44%), Query Frame = 0

Query: 18  EEEWYMRSSNLRRSGVVALVLVMIAIISACWI-IIVHRWNQRKKMRRGPKTWPIVGAAIE 77
           ++EWY       +SGV+ +   ++      W  +I+HRWNQRK    GPKTWP++GAA+E
Sbjct: 3   DQEWYS-----SKSGVLGVATCLV------WTWMIIHRWNQRK--NTGPKTWPLLGAALE 62

Query: 78  QFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSY 137
           Q +NYHRMHDW+LGYLNL +TVVVPMPFTTYTYIADPANVQHILKTNF NYPKGD YHSY
Sbjct: 63  QLLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSY 122

Query: 138 MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQ 197
           MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS LVFR+Y LKLS ILTRASL NQ
Sbjct: 123 MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQ 182

Query: 198 QIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIK 257
           Q+D+QELLMRMTLDSICKVGFGVEIGSLAPNLPEN FAKAFDTANMIVTLRFIDP WKIK
Sbjct: 183 QVDVQELLMRMTLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIK 242

Query: 258 RFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKHDILSRFIELGED 317
           RFLNLGSEALL KSIK ID FTYSVIATRKKEIQ+A TT   PNK+KHDILSRFIELGED
Sbjct: 243 RFLNLGSEALLQKSIKVIDHFTYSVIATRKKEIQQAPTT---PNKIKHDILSRFIELGED 302

Query: 318 PNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKE 377
           PNN FT+KSLRD+VLNFVIAGRDTTAATLSW+IYM+MTHS V+ ++YSELKSFEEERAKE
Sbjct: 303 PNNNFTNKSLRDVVLNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKE 362

Query: 378 HSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGIL 437
             ISLL + P+DIQSF+ RV QF++LLNYDSL RLHYLHA++TETLRLYPAVPQDPKG+L
Sbjct: 363 ERISLLHHHPSDIQSFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVL 422

Query: 438 EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAF 497
           EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWG DATSF P+RWLKQG+F NESPFKFTAF
Sbjct: 423 EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQGVFHNESPFKFTAF 482

Query: 498 QAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPRS 557
           QAGPRICMGKDSAYLQMKM LAILCRFYKFSLVSGH V YRMMTILSMANGLKVTVA RS
Sbjct: 483 QAGPRICMGKDSAYLQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 526

BLAST of CsaV3_2G010790 vs. NCBI nr
Match: XP_023545882.1 (cytochrome P450 704B1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 899.4 bits (2323), Expect = 1.5e-257
Identity = 446/540 (82.59%), Postives = 482/540 (89.26%), Query Frame = 0

Query: 18  EEEWYMRSSNLRRSGVVALVLVMIAIISACWI-IIVHRWNQRKKMRRGPKTWPIVGAAIE 77
           ++EWY       +SGV+ +   ++      W  +I+HRWNQRK    GPKTWP++GAA+E
Sbjct: 3   DQEWYS-----SKSGVLGVATCLV------WTWMIIHRWNQRK--NTGPKTWPLLGAALE 62

Query: 78  QFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSY 137
           Q +NYHRMHDW+LGYLNL +TVVVPMPFTTYTYIADPANVQHILKTNF NYPKGD YHSY
Sbjct: 63  QLLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSY 122

Query: 138 MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQ 197
           MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS LVFR+Y LKLS ILT+ASL NQ
Sbjct: 123 MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSGILTQASLHNQ 182

Query: 198 QIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIK 257
           Q+DMQELLMRMTLDSICKVGFGVEIGSLAPNLPEN FAKAFDTANMIVTLRFIDP WKIK
Sbjct: 183 QVDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIK 242

Query: 258 RFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKHDILSRFIELGED 317
           RFLNLGSEALL KSIK ID FTYSVIATRK EIQ+A TT   PNK+KHDILSRFIELGED
Sbjct: 243 RFLNLGSEALLQKSIKVIDHFTYSVIATRKNEIQQAPTT---PNKIKHDILSRFIELGED 302

Query: 318 PNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKE 377
           PNN FT+KSLRD+VLNFVIAGRDTTAATLSW+IYM+MTHS V+ ++YSELKSFEEERAKE
Sbjct: 303 PNNNFTNKSLRDVVLNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKE 362

Query: 378 HSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGIL 437
             ISLL + P+DIQSF+ RV QF++LLNYDSL RLHYLHA++TETLRLYPAVPQDPKG+L
Sbjct: 363 EGISLLHHHPSDIQSFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVL 422

Query: 438 EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAF 497
           EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWG DATSF PERWLKQG+F NESPFKFTAF
Sbjct: 423 EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGSDATSFNPERWLKQGVFHNESPFKFTAF 482

Query: 498 QAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPRS 557
           QAGPRICMGKDSAYLQMKM LAILCRFYKFSLVSGH V YRMMTILSMANGLKVTVA RS
Sbjct: 483 QAGPRICMGKDSAYLQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 526

BLAST of CsaV3_2G010790 vs. ExPASy Swiss-Prot
Match: Q9C788 (Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 1.0e-227
Identity = 386/523 (73.80%), Postives = 448/523 (85.66%), Query Frame = 0

Query: 39  VMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTV 98
           ++IA +   WI + HRW QR K   GPKTWP+VGAAIEQ  N+ RMHDW++ YL   RTV
Sbjct: 5   LVIACMVTSWIFL-HRWGQRNK--SGPKTWPLVGAAIEQLTNFDRMHDWLVEYLYNSRTV 64

Query: 99  VVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRK 158
           VVPMPFTTYTYIADP NV+++LKTNF+NYPKG+ YHSYMEVLLGDGIFN DGELWRKQRK
Sbjct: 65  VVPMPFTTYTYIADPINVEYVLKTNFSNYPKGETYHSYMEVLLGDGIFNSDGELWRKQRK 124

Query: 159 TASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFG 218
           TASFEFASKNLRDFST+VF++Y LKL TIL++AS   QQ+DMQELLMRMTLDSICKVGFG
Sbjct: 125 TASFEFASKNLRDFSTVVFKEYSLKLFTILSQASFKEQQVDMQELLMRMTLDSICKVGFG 184

Query: 219 VEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFT 278
           VEIG+LAP LPEN FAKAFDTAN+IVTLRFIDPLWK+K+FLN+GSEALL KSIK ++DFT
Sbjct: 185 VEIGTLAPELPENHFAKAFDTANIIVTLRFIDPLWKMKKFLNIGSEALLGKSIKVVNDFT 244

Query: 279 YSVIATRKKEIQEAQTTHT-----DPNKMKHDILSRFIELGEDPNNKFTDKSLRDIVLNF 338
           YSVI  RK E+ EAQ + T     + NK+KHDILSRFIE+ +DP++K T+KSLRDIVLNF
Sbjct: 245 YSVIRRRKAELLEAQISPTNNNNNNNNKVKHDILSRFIEISDDPDSKETEKSLRDIVLNF 304

Query: 339 VIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSFD 398
           VIAGRDTTA TL+W+IYM+M + +V++KLYSEL+  E+E A+  + SL QY   D  SF+
Sbjct: 305 VIAGRDTTATTLTWAIYMIMMNENVAEKLYSELQELEKESAEATNTSLHQYDTEDFNSFN 364

Query: 399 TRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMV 458
            +V +F+ LLNYDSL +LHYLHA++TETLRLYPAVPQDPKG+LEDD+LP+GTKV+AGGMV
Sbjct: 365 EKVTEFAGLLNYDSLGKLHYLHAVITETLRLYPAVPQDPKGVLEDDMLPNGTKVKAGGMV 424

Query: 459 TYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAFQAGPRICMGKDSAYLQM 518
           TYVPYSMGRMEYNWG DA  FKPERWLK G+FQN SPFKFTAFQAGPRIC+GKDSAYLQM
Sbjct: 425 TYVPYSMGRMEYNWGSDAALFKPERWLKDGVFQNASPFKFTAFQAGPRICLGKDSAYLQM 484

Query: 519 KMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPRS 557
           KM +AILCRFYKF LV  H V YRMMTILSMA+GLKVTV+ RS
Sbjct: 485 KMAMAILCRFYKFHLVPNHPVKYRMMTILSMAHGLKVTVSRRS 524

BLAST of CsaV3_2G010790 vs. ExPASy Swiss-Prot
Match: Q50EK3 (Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.1e-128
Identity = 252/530 (47.55%), Postives = 338/530 (63.77%), Query Frame = 0

Query: 33  VVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYL 92
           ++ + + + A+  AC + I       +K    P   P+VG  +   +N+ R+HD+     
Sbjct: 5   ILTMFVTVSALALACSLWIASYLRNWRKKGVYP---PVVGTMLNHAINFERLHDYHTDQA 64

Query: 93  NLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGEL 152
             Y+T  V  P  +Y +  DP NV+HILKTNFANY KG   +  M+ LLGDGIFNVDG+ 
Sbjct: 65  QRYKTFRVVYPTCSYVFTTDPVNVEHILKTNFANYDKGTFNYDIMKDLLGDGIFNVDGDK 124

Query: 153 WRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQIDMQELLMRMTLDSI 212
           WR+QRK AS EFASK L+DFS+ VF +   KL+ IL +A+ LN  ++MQ+L MR +LDSI
Sbjct: 125 WRQQRKLASSEFASKVLKDFSSGVFCNNAAKLANILAQAAKLNLSVEMQDLFMRSSLDSI 184

Query: 213 CKVGFGVEIGSL----APNLPENKFAKAFDTANMIVTLR-FIDPLWKIKRFLNLGSEALL 272
           CKV FG++I SL    A + PE  FAKAFD AN +V  R  +   WK++RF N+GSEA+L
Sbjct: 185 CKVVFGIDINSLSSSKAESGPEASFAKAFDVANAMVFHRHMVGSFWKVQRFFNVGSEAIL 244

Query: 273 DKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKHDILSRFIELGE-DPNNKFTDKSL 332
             +IK +DDF Y VI  R++E+  A+  +  P     DILSR+I + + + + K +DK L
Sbjct: 245 RDNIKMVDDFLYKVIHFRRQEMFSAEKENVRP-----DILSRYIIISDKETDGKVSDKYL 304

Query: 333 RDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKEHSISLLQYQP 392
           RD++LNF++A RDTTA  LSW IYML  H  V +KL  E+ S       ++S      + 
Sbjct: 305 RDVILNFMVAARDTTAIALSWFIYMLCKHQHVQEKLLEEIISSTSVHEDQYST-----EC 364

Query: 393 NDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGILEDDVLPDGTK 452
           NDI S       F+Q L  ++L ++HYLHA ++ETLRLYPA+P D K ++ +D LPDG K
Sbjct: 365 NDIAS-------FAQSLTDEALGKMHYLHASLSETLRLYPALPVDGKYVVNEDTLPDGFK 424

Query: 453 VRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAFQAGPRICMGK 512
           V+ G  V ++PY+MGRM Y WG DA  FKPERW++ GIF  +SPFKF AFQAGPR C+GK
Sbjct: 425 VKKGDSVNFLPYAMGRMSYLWGDDAKEFKPERWIQDGIFHPKSPFKFPAFQAGPRTCLGK 484

Query: 513 DSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSM-ANGLKVTVAPR 556
           D AYLQMK+  A+L RF+KF  V    V YR M  L M  +GL V V PR
Sbjct: 485 DFAYLQMKIVAAVLVRFFKFEAVKTKEVRYRTMLTLHMNEDGLNVQVTPR 514

BLAST of CsaV3_2G010790 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 3.8e-102
Identity = 210/538 (39.03%), Postives = 309/538 (57.43%), Query Frame = 0

Query: 34  VALVLVMIAIISACWIIIVHRWNQR-KKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYL 93
           V+  ++++A+++A W+     W QR  +  +GP+ WP++G+         RMHDWI   L
Sbjct: 3   VSNTMLLVAVVAAYWL-----WFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENL 62

Query: 94  ----NLYRTVVVPMPFTT-----YTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGD 153
                 Y+T +  +PF        T   DP N++H+LKT F NYPKG  + +     LG 
Sbjct: 63  RACGGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQ 122

Query: 154 GIFNVDGELWRKQRKTASFEFASKNLRD-FSTLVFRDYGLKLSTILTRASLLNQQIDMQE 213
           GIFN DG+ W  QRKTA+ EF ++ LR      V R   L+   IL  A    + +D+Q+
Sbjct: 123 GIFNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQD 182

Query: 214 LLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDP--LWKIKRFLN 273
           L++R+T D+IC + FG +  + AP LPEN FA AFD A      RFI P  LW++K++L 
Sbjct: 183 LILRLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLG 242

Query: 274 LGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKH-DILSRFIELGEDPNN 333
           LG E  L +S+  ID +  +VI TRK+E+     +  +    +H D+LSRF+   +  + 
Sbjct: 243 LGLEVSLSRSLGEIDGYLDAVINTRKQEL----LSQRESGVQRHDDLLSRFM---KKKDQ 302

Query: 334 KFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKEHSI 393
            +++  LR + LNF++AGRDT++  LSW  +++ TH  V  K+  E+ S           
Sbjct: 303 SYSETFLRHVALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSV---------- 362

Query: 394 SLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGILEDD 453
            L++ +  D+ S+       ++ L +D + RL YL A ++ETLRLYP+VP+D K ++ DD
Sbjct: 363 -LIETRGTDVSSWT------AEPLEFDEVDRLVYLKAALSETLRLYPSVPEDSKHVVNDD 422

Query: 454 VLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLK--QGIFQNESPFKFTAFQ 513
           +LPDGT V AG  VTY  Y+ GRM+  WG D   FKPERW+    G F N   ++F AF 
Sbjct: 423 ILPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGKFVNHDQYRFVAFN 482

Query: 514 AGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPR 556
           AGPRIC+GKD AYLQMK   A +   ++ ++  GH+V+ +M   L M NGL V V  R
Sbjct: 483 AGPRICLGKDLAYLQMKTIAAAVLLRHRLTVAPGHKVEQKMSLTLFMKNGLLVNVHKR 511

BLAST of CsaV3_2G010790 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 5.5e-101
Identity = 212/540 (39.26%), Postives = 318/540 (58.89%), Query Frame = 0

Query: 32  GVVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGY 91
           G  A +++ + +    W + + R        +GP+ WP+VG+      N HRMHD+I   
Sbjct: 3   GSTAAIILTLIVTYIIWFVSLRR------SYKGPRVWPLVGSLPALITNAHRMHDFIADN 62

Query: 92  LNL----YRTVVVPMPFTT-----YTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLG 151
           L +    Y+T + P+PF        T   DP N++HILKT F NYPKG  + S    LLG
Sbjct: 63  LRMCGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLG 122

Query: 152 DGIFNVDGELWRKQRKTASFEFASKNLRD-FSTLVFRDYGLKLSTILTRASLLNQQIDMQ 211
           DGIFN DG+ WR QRKTA+ EF ++ LR   +  V R    +L  IL  A    + ID+Q
Sbjct: 123 DGIFNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQ 182

Query: 212 ELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDP--LWKIKRFL 271
           ++L+R+T D+IC + FG +  +L+P  PEN FA AFD A      RFI P  +WKI+++L
Sbjct: 183 DVLLRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWL 242

Query: 272 NLGSEALLDKSIKTIDDFTYSVIATRKKEI---QEAQTTHTDPNKMKHDILSRFIELGED 331
            LG E  + +SI  +D++   +I TRK E+   Q+ ++ H        D+LSRF++  E 
Sbjct: 243 RLGLEDDMSRSISHVDNYLSEIINTRKLELLGQQQDESRH-------DDLLSRFMKKKE- 302

Query: 332 PNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKE 391
               ++DK L+ + LNF++AGRDT++  +SW  +++  +  V +K+ +E+          
Sbjct: 303 ---SYSDKYLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEI---------- 362

Query: 392 HSISLLQYQPNDIQSFDTRVVQFS-QLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGI 451
              ++L      I++ DT V +++ + L +D + +L YL A ++ETLRLYP+VP+D K +
Sbjct: 363 --CTIL------IKTRDTNVSKWTDEPLTFDEIDQLVYLKAALSETLRLYPSVPEDSKFV 422

Query: 452 LEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTA 511
           + +DVLPDGT V +G  VTY  YS+GRM++ WG D   FKPERWL++   +  + +KF A
Sbjct: 423 VANDVLPDGTFVPSGSNVTYSIYSVGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVA 482

Query: 512 FQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPR 556
           F AGPRIC+GKD AYLQMK   A +   ++ ++  GHRV+ +M   L M  GLK+ V  R
Sbjct: 483 FNAGPRICLGKDLAYLQMKSITASILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKR 507

BLAST of CsaV3_2G010790 vs. ExPASy Swiss-Prot
Match: O80823 (Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 5.5e-101
Identity = 217/541 (40.11%), Postives = 311/541 (57.49%), Query Frame = 0

Query: 34  VALVLVMIAIISA--CWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGY 93
           ++  L++++ I+A   W+  + R        +GP+ WPI+G+      N  RMHDWI   
Sbjct: 3   ISTALMILSAITAYFLWLTFISR------CLKGPRVWPILGSLPGLIENCERMHDWISDN 62

Query: 94  L----NLYRTVVVPMPFTT-----YTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLG 153
           L      Y+T +  +PF        T   DP N++HILK  F NYPKG  + +    LLG
Sbjct: 63  LRACSGTYQTCICAIPFLAKKQGLVTVTCDPRNLEHILKNRFDNYPKGPTWQAVFHDLLG 122

Query: 154 DGIFNVDGELWRKQRKTASFEFASKNLRD-FSTLVFRDYGLKLSTILTRASLLNQQIDMQ 213
            GIFN DG+ W  QRKTA+ EF ++ LR   +  V R   L+   IL  A L ++ ID+Q
Sbjct: 123 QGIFNSDGDTWLFQRKTAALEFTTRTLRQAMARWVNRAIKLRFLPILENARLGSEPIDLQ 182

Query: 214 ELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDP--LWKIKRFL 273
           +LL+R+T D+IC + FG +  + AP LP N FA AFD A      RFI P  LWK KR+L
Sbjct: 183 DLLLRLTFDNICGLTFGKDPRTCAPGLPVNTFAVAFDRATEASLQRFILPEILWKFKRWL 242

Query: 274 NLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKH--DILSRFIELGEDP 333
            LG E  L +S+  +D++   +I TRK+E+       T  N  KH  D+LSRFI+  E  
Sbjct: 243 RLGLEVSLTRSLVQVDNYLSEIITTRKEEMM------TQHNNGKHHDDLLSRFIKKKE-- 302

Query: 334 NNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKEH 393
              ++D++L+ + LNF++AGRDT++  LSW  +++  H  +  K+  E+ +         
Sbjct: 303 --SYSDETLQRVALNFILAGRDTSSVALSWFFWLITQHPAIEDKILREICTV-------- 362

Query: 394 SISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGILE 453
              L++ + +D+       +   + L+ + L RL +L A ++ETLRLYP+VP+D K  ++
Sbjct: 363 ---LVETRGDDV------ALWTDEPLSCEELDRLVFLKAALSETLRLYPSVPEDSKRAVK 422

Query: 454 DDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQ---GIFQNESPFKFT 513
           DDVLPDGT V AG  +TY  YS GRM+  WG D   FKPERW+ Q   G F N  PFKF 
Sbjct: 423 DDVLPDGTFVPAGSSITYSIYSAGRMKSTWGEDCLEFKPERWISQSDGGRFINHDPFKFV 482

Query: 514 AFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAP 556
           AF AGPRIC+GKD AYLQMK   + +   ++ ++V+GH+V+ +M   L M  GL V V  
Sbjct: 483 AFNAGPRICLGKDLAYLQMKSIASAVLLRHRLTVVTGHKVEQKMSLTLFMKYGLLVNVHE 510

BLAST of CsaV3_2G010790 vs. ExPASy TrEMBL
Match: A0A0A0LN46 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108690 PE=3 SV=1)

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60
           MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK
Sbjct: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60

Query: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120
           MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL
Sbjct: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120

Query: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180
           KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY
Sbjct: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180

Query: 181 GLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA 240
           GLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA
Sbjct: 181 GLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA 240

Query: 241 NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPN 300
           NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPN
Sbjct: 241 NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPN 300

Query: 301 KMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQ 360
           KMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQ
Sbjct: 301 KMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQ 360

Query: 361 KLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTE 420
           KLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTE
Sbjct: 361 KLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTE 420

Query: 421 TLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWL 480
           TLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWL
Sbjct: 421 TLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWL 480

Query: 481 KQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMT 540
           KQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMT
Sbjct: 481 KQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMT 540

Query: 541 ILSMANGLKVTVAPRS 557
           ILSMANGLKVTVAPRS
Sbjct: 541 ILSMANGLKVTVAPRS 556

BLAST of CsaV3_2G010790 vs. ExPASy TrEMBL
Match: A0A1S3B4S2 (cytochrome P450 704B1 OS=Cucumis melo OX=3656 GN=LOC103485770 PE=3 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.5e-306
Identity = 526/557 (94.43%), Postives = 542/557 (97.31%), Query Frame = 0

Query: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60
           MGISLLEWCNNNNRA+SEEEW+MRSSNLRRS VV LVL +I I++ CWIIIVHRWN+RKK
Sbjct: 1   MGISLLEWCNNNNRANSEEEWHMRSSNLRRSVVVVLVLGVITIVT-CWIIIVHRWNERKK 60

Query: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120
           MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLY+TVVVPMPFTTYTYIADPANVQHIL
Sbjct: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYKTVVVPMPFTTYTYIADPANVQHIL 120

Query: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180
           KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY
Sbjct: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180

Query: 181 GLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA 240
            LKLSTILTRASLLNQQ+DMQELL RMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA
Sbjct: 181 SLKLSTILTRASLLNQQLDMQELLTRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTA 240

Query: 241 NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDP- 300
           NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIK IDDFTYSVIATRKKEIQEAQTT T+P 
Sbjct: 241 NMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKIIDDFTYSVIATRKKEIQEAQTTPTEPN 300

Query: 301 NKMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVS 360
           NK+KHDILSRFIELGEDPNNKFTDKSLRD+VLNFVIAGRDTTAATLSWSIYMLMTHSDVS
Sbjct: 301 NKIKHDILSRFIELGEDPNNKFTDKSLRDVVLNFVIAGRDTTAATLSWSIYMLMTHSDVS 360

Query: 361 QKLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVT 420
           QKLYSELKSFEEERAKEHSISLLQYQPNDIQSF+ RV+QFSQLLNYDSLARLHYLHAI+T
Sbjct: 361 QKLYSELKSFEEERAKEHSISLLQYQPNDIQSFERRVIQFSQLLNYDSLARLHYLHAIIT 420

Query: 421 ETLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERW 480
           ETLRLYPAVPQDPKGILEDD+LPDGTKVRAGGMVTYVPYSMGRMEYNWG DATSF PERW
Sbjct: 421 ETLRLYPAVPQDPKGILEDDILPDGTKVRAGGMVTYVPYSMGRMEYNWGPDATSFNPERW 480

Query: 481 LKQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMM 540
           LKQGIF NESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRV+YRMM
Sbjct: 481 LKQGIFHNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVNYRMM 540

Query: 541 TILSMANGLKVTVAPRS 557
           TILSMANGLKVTVA RS
Sbjct: 541 TILSMANGLKVTVASRS 556

BLAST of CsaV3_2G010790 vs. ExPASy TrEMBL
Match: A0A5D3BJX9 (Cytochrome P450 704B1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold316G00130 PE=3 SV=1)

HSP 1 Score: 977.6 bits (2526), Expect = 2.1e-281
Identity = 482/504 (95.63%), Postives = 494/504 (98.02%), Query Frame = 0

Query: 54  RWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADP 113
           +WN+RKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLY+TVVVPMPFTTYTYIADP
Sbjct: 6   KWNERKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYKTVVVPMPFTTYTYIADP 65

Query: 114 ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS 173
           ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS
Sbjct: 66  ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS 125

Query: 174 TLVFRDYGLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKF 233
           TLVFRDY LKLSTILTRASLLNQQ+DMQELL RMTLDSICKVGFGVEIGSLAPNLPENKF
Sbjct: 126 TLVFRDYSLKLSTILTRASLLNQQLDMQELLTRMTLDSICKVGFGVEIGSLAPNLPENKF 185

Query: 234 AKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQ 293
           AKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIK IDDFTYSVIATRKKEIQEAQ
Sbjct: 186 AKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKIIDDFTYSVIATRKKEIQEAQ 245

Query: 294 TTHTDP-NKMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYML 353
           TT T+P NK+KHDILSRFIELGEDPNNKFTDKSLRD+VLNFVIAGRDTTAATLSWSIYML
Sbjct: 246 TTPTEPNNKIKHDILSRFIELGEDPNNKFTDKSLRDVVLNFVIAGRDTTAATLSWSIYML 305

Query: 354 MTHSDVSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLH 413
           MTHSDVSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSF+ RV+QFSQLLNYDSLARLH
Sbjct: 306 MTHSDVSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSFERRVIQFSQLLNYDSLARLH 365

Query: 414 YLHAIVTETLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDAT 473
           YLHAI+TETLRLYPAVPQDPKGILEDD+LPDGTKVRAGGMVTYVPYSMGRMEYNWG DAT
Sbjct: 366 YLHAIITETLRLYPAVPQDPKGILEDDILPDGTKVRAGGMVTYVPYSMGRMEYNWGPDAT 425

Query: 474 SFKPERWLKQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGH 533
           SF PERWLKQGIF NESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGH
Sbjct: 426 SFNPERWLKQGIFHNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGH 485

Query: 534 RVDYRMMTILSMANGLKVTVAPRS 557
           RV+YRMMTILSMANGLKVTVA RS
Sbjct: 486 RVNYRMMTILSMANGLKVTVASRS 509

BLAST of CsaV3_2G010790 vs. ExPASy TrEMBL
Match: A0A6J1G378 (cytochrome P450 704B1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450376 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.3e-258
Identity = 446/540 (82.59%), Postives = 483/540 (89.44%), Query Frame = 0

Query: 18  EEEWYMRSSNLRRSGVVALVLVMIAIISACWI-IIVHRWNQRKKMRRGPKTWPIVGAAIE 77
           ++EWY       +SGV+ +   ++      W  +I+HRWNQRK    GPKTWP++GAA+E
Sbjct: 3   DQEWYS-----SKSGVLGVATCLV------WTWMIIHRWNQRK--NTGPKTWPLLGAALE 62

Query: 78  QFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSY 137
           Q +NYHRMHDW+LGYLNL +TVVVPMPFTTYTYIADPANVQHILKTNF NYPKGD YHSY
Sbjct: 63  QLLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSY 122

Query: 138 MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQ 197
           MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS LVFR+Y LKLS ILTRASL NQ
Sbjct: 123 MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQ 182

Query: 198 QIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIK 257
           Q+D+QELLMRMTLDSICKVGFGVEIGSLAPNLPEN FAKAFDTANMIVTLRFIDP WKIK
Sbjct: 183 QVDVQELLMRMTLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIK 242

Query: 258 RFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKHDILSRFIELGED 317
           RFLNLGSEALL KSIK ID FTYSVIATRKKEIQ+A TT   PNK+KHDILSRFIELGED
Sbjct: 243 RFLNLGSEALLQKSIKVIDHFTYSVIATRKKEIQQAPTT---PNKIKHDILSRFIELGED 302

Query: 318 PNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKE 377
           PNN FT+KSLRD+VLNFVIAGRDTTAATLSW+IYM+MTHS V+ ++YSELKSFEEERAKE
Sbjct: 303 PNNNFTNKSLRDVVLNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKE 362

Query: 378 HSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGIL 437
             ISLL + P+DIQSF+ RV QF++LLNYDSL RLHYLHA++TETLRLYPAVPQDPKG+L
Sbjct: 363 ERISLLHHHPSDIQSFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVL 422

Query: 438 EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAF 497
           EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWG DATSF P+RWLKQG+F NESPFKFTAF
Sbjct: 423 EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQGVFHNESPFKFTAF 482

Query: 498 QAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPRS 557
           QAGPRICMGKDSAYLQMKM LAILCRFYKFSLVSGH V YRMMTILSMANGLKVTVA RS
Sbjct: 483 QAGPRICMGKDSAYLQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 526

BLAST of CsaV3_2G010790 vs. ExPASy TrEMBL
Match: A0A6J1ICQ4 (cytochrome P450 704B1 OS=Cucurbita maxima OX=3661 GN=LOC111471352 PE=3 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.7e-257
Identity = 444/540 (82.22%), Postives = 482/540 (89.26%), Query Frame = 0

Query: 18  EEEWYMRSSNLRRSGVVALVLVMIAIISACWI-IIVHRWNQRKKMRRGPKTWPIVGAAIE 77
           ++EWY       +SGV+ +   ++      W  +I+HRWNQRK    GPKTWP++GAA+E
Sbjct: 3   DQEWYS-----SKSGVLGVATCLV------WTWMIIHRWNQRK--NTGPKTWPLLGAALE 62

Query: 78  QFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSY 137
           Q +NYHRMHDW+LGYLNL +TVVVPMPFTTYTYIADPANVQHILKTNF NYPKGD YHSY
Sbjct: 63  QLLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSY 122

Query: 138 MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQ 197
           MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS LVFR+Y LKLS ILTRASL NQ
Sbjct: 123 MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQ 182

Query: 198 QIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIK 257
           Q+D+QELLMRMTLDSICKVGFGVEIGSLAPNLPEN FAKAFDTANMIVTLRFIDP WKIK
Sbjct: 183 QVDIQELLMRMTLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIK 242

Query: 258 RFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKHDILSRFIELGED 317
           RFLN+GSEALL KSIK ID FTYSVIATRKKEIQ+A TT   PNK+KHDILSRFIELGED
Sbjct: 243 RFLNMGSEALLQKSIKVIDHFTYSVIATRKKEIQQAPTT---PNKIKHDILSRFIELGED 302

Query: 318 PNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKE 377
           PNN FT+KSLRD+VLNFVIAGRDTTAATLSW+IYM+MTHS V+ ++YSELKSFEEERAKE
Sbjct: 303 PNNNFTNKSLRDVVLNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKE 362

Query: 378 HSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGIL 437
             ISLL + P+DIQSF+ RV QF++LLNYDSL RLHYLHA++TETLRLYPAVPQDPKG+L
Sbjct: 363 EGISLLHHHPSDIQSFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVL 422

Query: 438 EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAF 497
           EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWG DATSF P+RWLKQ +F NESPFKFTAF
Sbjct: 423 EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAF 482

Query: 498 QAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPRS 557
           QAGPRICMGKDSAYLQMKM LAILCRFYKFSLVSGH V YRMMTILSMANGLKVTVA RS
Sbjct: 483 QAGPRICMGKDSAYLQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 526

BLAST of CsaV3_2G010790 vs. TAIR 10
Match: AT1G69500.1 (cytochrome P450, family 704, subfamily B, polypeptide 1 )

HSP 1 Score: 790.8 bits (2041), Expect = 7.1e-229
Identity = 386/523 (73.80%), Postives = 448/523 (85.66%), Query Frame = 0

Query: 39  VMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTV 98
           ++IA +   WI + HRW QR K   GPKTWP+VGAAIEQ  N+ RMHDW++ YL   RTV
Sbjct: 5   LVIACMVTSWIFL-HRWGQRNK--SGPKTWPLVGAAIEQLTNFDRMHDWLVEYLYNSRTV 64

Query: 99  VVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRK 158
           VVPMPFTTYTYIADP NV+++LKTNF+NYPKG+ YHSYMEVLLGDGIFN DGELWRKQRK
Sbjct: 65  VVPMPFTTYTYIADPINVEYVLKTNFSNYPKGETYHSYMEVLLGDGIFNSDGELWRKQRK 124

Query: 159 TASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFG 218
           TASFEFASKNLRDFST+VF++Y LKL TIL++AS   QQ+DMQELLMRMTLDSICKVGFG
Sbjct: 125 TASFEFASKNLRDFSTVVFKEYSLKLFTILSQASFKEQQVDMQELLMRMTLDSICKVGFG 184

Query: 219 VEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFT 278
           VEIG+LAP LPEN FAKAFDTAN+IVTLRFIDPLWK+K+FLN+GSEALL KSIK ++DFT
Sbjct: 185 VEIGTLAPELPENHFAKAFDTANIIVTLRFIDPLWKMKKFLNIGSEALLGKSIKVVNDFT 244

Query: 279 YSVIATRKKEIQEAQTTHT-----DPNKMKHDILSRFIELGEDPNNKFTDKSLRDIVLNF 338
           YSVI  RK E+ EAQ + T     + NK+KHDILSRFIE+ +DP++K T+KSLRDIVLNF
Sbjct: 245 YSVIRRRKAELLEAQISPTNNNNNNNNKVKHDILSRFIEISDDPDSKETEKSLRDIVLNF 304

Query: 339 VIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSFD 398
           VIAGRDTTA TL+W+IYM+M + +V++KLYSEL+  E+E A+  + SL QY   D  SF+
Sbjct: 305 VIAGRDTTATTLTWAIYMIMMNENVAEKLYSELQELEKESAEATNTSLHQYDTEDFNSFN 364

Query: 399 TRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMV 458
            +V +F+ LLNYDSL +LHYLHA++TETLRLYPAVPQDPKG+LEDD+LP+GTKV+AGGMV
Sbjct: 365 EKVTEFAGLLNYDSLGKLHYLHAVITETLRLYPAVPQDPKGVLEDDMLPNGTKVKAGGMV 424

Query: 459 TYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAFQAGPRICMGKDSAYLQM 518
           TYVPYSMGRMEYNWG DA  FKPERWLK G+FQN SPFKFTAFQAGPRIC+GKDSAYLQM
Sbjct: 425 TYVPYSMGRMEYNWGSDAALFKPERWLKDGVFQNASPFKFTAFQAGPRICLGKDSAYLQM 484

Query: 519 KMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPRS 557
           KM +AILCRFYKF LV  H V YRMMTILSMA+GLKVTV+ RS
Sbjct: 485 KMAMAILCRFYKFHLVPNHPVKYRMMTILSMAHGLKVTVSRRS 524

BLAST of CsaV3_2G010790 vs. TAIR 10
Match: AT2G45510.1 (cytochrome P450, family 704, subfamily A, polypeptide 2 )

HSP 1 Score: 399.8 bits (1026), Expect = 3.5e-111
Identity = 231/528 (43.75%), Postives = 308/528 (58.33%), Query Frame = 0

Query: 34  VALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLN 93
           +A+ +     I  C+ I +       K R   +  P+     +   +   ++D+      
Sbjct: 7   IAITVATTIFIVLCFTIYLMIRIFTGKSRNDKRYAPVHATVFDLLFHSDELYDYETEIAR 66

Query: 94  LYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELW 153
              T     P  +    ADP NV+HILKT F NY KG      M  LLG GIF VDGE W
Sbjct: 67  EKPTYRFLSPGQSEILTADPRNVEHILKTRFDNYSKGHSSRENMADLLGHGIFAVDGEKW 126

Query: 154 RKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQIDMQELLMRMTLDSIC 213
           R+QRK +SFEF+++ LRDFS  VFR    KL   ++  +L  +  D Q+LLMR TLDSI 
Sbjct: 127 RQQRKLSSFEFSTRVLRDFSCSVFRRNASKLVGFVSEFALSGKAFDAQDLLMRCTLDSIF 186

Query: 214 KVGFGVEIGSLAPNLPE-NKFAKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIK 273
           KVGFGVE+  L     E  +F +AFD  N+  + RFIDPLWK+K F N+GS++ L KSI 
Sbjct: 187 KVGFGVELKCLDGFSKEGQEFMEAFDEGNVATSSRFIDPLWKLKWFFNIGSQSKLKKSIA 246

Query: 274 TIDDFTYSVIATRKKEIQEAQTTHTDPNKMKHDILSRF-IELGEDPNNKFTDKSLRDIVL 333
           TID F YS+I T++KE+ + Q T      ++ DILSRF +E  +DP N   DK LRDI+L
Sbjct: 247 TIDKFVYSLITTKRKELAKEQNT-----VVREDILSRFLVESEKDPEN-MNDKYLRDIIL 306

Query: 334 NFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELK--SFEEERAKEHSISLLQYQPNDI 393
           NF+IAG+DTTAA LSW +YML  +  V +K+  E++  +F  E+                
Sbjct: 307 NFMIAGKDTTAALLSWFLYMLCKNPLVQEKIVQEIRDVTFSHEKT--------------- 366

Query: 394 QSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGILEDDVLPDGTKVRA 453
               T V  F + +N ++L  +HYLHA ++ETLRLYP VP D +    DDVLPDG +V  
Sbjct: 367 ----TDVNGFVESINEEALDEMHYLHAALSETLRLYPPVPVDMRCAENDDVLPDGHRVSK 426

Query: 454 GGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAFQAGPRICMGKDSA 513
           G  + Y+ Y+MGRM Y WG DA  FKPERWLK G+FQ ESPFKF +F AGPRIC+GKD A
Sbjct: 427 GDNIYYIAYAMGRMTYIWGQDAEEFKPERWLKDGLFQPESPFKFISFHAGPRICLGKDFA 486

Query: 514 YLQMKMTLAILCRFYKFSLV-SGHRVDYRMMTILSMANGLKVTVAPRS 557
           Y QMK+    L  F++F +     +V Y+ M  L +  GL +   PR+
Sbjct: 487 YRQMKIVSMALLHFFRFKMADENSKVYYKRMLTLHVDGGLHLCAIPRT 509

BLAST of CsaV3_2G010790 vs. TAIR 10
Match: AT2G44890.1 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 392.1 bits (1006), Expect = 7.4e-109
Identity = 217/500 (43.40%), Postives = 295/500 (59.00%), Query Frame = 0

Query: 60  KMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHI 119
           K R   +  P+     + F + H+++D+         T     P  +  + ADP NV+HI
Sbjct: 27  KSRNDKRYTPVHATIFDLFFHSHKLYDYETEIARTKPTFRFLSPGQSEIFTADPRNVEHI 86

Query: 120 LKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRD 179
           LKT F NY KG V    +  LLG GIF VDGE W++QRK  SFEF+++ LR+FS  VFR 
Sbjct: 87  LKTRFHNYSKGPVGTVNLADLLGHGIFAVDGEKWKQQRKLVSFEFSTRVLRNFSYSVFRT 146

Query: 180 YGLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPE-NKFAKAFD 239
              KL   +   +L  +  D Q++LM+ TLDSI KVGFGVE+G L     E  +F KAFD
Sbjct: 147 SASKLVGFIAEFALSGKSFDFQDMLMKCTLDSIFKVGFGVELGCLDGFSKEGEEFMKAFD 206

Query: 240 TANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTD 299
             N   + R  DP WK+K FLN+GSE+ L KSI  ID F YS+I T++KE+ + Q T   
Sbjct: 207 EGNGATSSRVTDPFWKLKCFLNIGSESRLKKSIAIIDKFVYSLITTKRKELSKEQNT--- 266

Query: 300 PNKMKHDILSRF-IELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSD 359
              ++ DILS+F +E  +DP N   DK LRDI+LN ++AG+DTTAA+LSW +YML  +  
Sbjct: 267 --SVREDILSKFLLESEKDPEN-MNDKYLRDIILNVMVAGKDTTAASLSWFLYMLCKNPL 326

Query: 360 VSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAI 419
           V +K+  E++       K                  T V  F + +  ++LA++ YLHA 
Sbjct: 327 VQEKIVQEIRDVTSSHEK-----------------TTDVNGFIESVTEEALAQMQYLHAA 386

Query: 420 VTETLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPE 479
           ++ET+RLYP VP+  +    DDVLPDG +V  G  + Y+ Y+MGRM Y WG DA  FKPE
Sbjct: 387 LSETMRLYPPVPEHMRCAENDDVLPDGHRVSKGDNIYYISYAMGRMTYIWGQDAEEFKPE 446

Query: 480 RWLKQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLV-SGHRVDY 539
           RWLK G+FQ ES FKF +F AGPRIC+GKD AY QMK+    L  F++F +     +V Y
Sbjct: 447 RWLKDGVFQPESQFKFISFHAGPRICIGKDFAYRQMKIVSMALLHFFRFKMADENSKVSY 503

Query: 540 RMMTILSMANGLKVTVAPRS 557
           + M  L +  GL +   PR+
Sbjct: 507 KKMLTLHVDGGLHLCAIPRT 503

BLAST of CsaV3_2G010790 vs. TAIR 10
Match: AT4G00360.1 (cytochrome P450, family 86, subfamily A, polypeptide 2 )

HSP 1 Score: 373.6 bits (958), Expect = 2.7e-103
Identity = 210/538 (39.03%), Postives = 309/538 (57.43%), Query Frame = 0

Query: 34  VALVLVMIAIISACWIIIVHRWNQR-KKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYL 93
           V+  ++++A+++A W+     W QR  +  +GP+ WP++G+         RMHDWI   L
Sbjct: 3   VSNTMLLVAVVAAYWL-----WFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENL 62

Query: 94  ----NLYRTVVVPMPFTT-----YTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGD 153
                 Y+T +  +PF        T   DP N++H+LKT F NYPKG  + +     LG 
Sbjct: 63  RACGGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQ 122

Query: 154 GIFNVDGELWRKQRKTASFEFASKNLRD-FSTLVFRDYGLKLSTILTRASLLNQQIDMQE 213
           GIFN DG+ W  QRKTA+ EF ++ LR      V R   L+   IL  A    + +D+Q+
Sbjct: 123 GIFNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQD 182

Query: 214 LLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDP--LWKIKRFLN 273
           L++R+T D+IC + FG +  + AP LPEN FA AFD A      RFI P  LW++K++L 
Sbjct: 183 LILRLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLG 242

Query: 274 LGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKH-DILSRFIELGEDPNN 333
           LG E  L +S+  ID +  +VI TRK+E+     +  +    +H D+LSRF+   +  + 
Sbjct: 243 LGLEVSLSRSLGEIDGYLDAVINTRKQEL----LSQRESGVQRHDDLLSRFM---KKKDQ 302

Query: 334 KFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKEHSI 393
            +++  LR + LNF++AGRDT++  LSW  +++ TH  V  K+  E+ S           
Sbjct: 303 SYSETFLRHVALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSV---------- 362

Query: 394 SLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGILEDD 453
            L++ +  D+ S+       ++ L +D + RL YL A ++ETLRLYP+VP+D K ++ DD
Sbjct: 363 -LIETRGTDVSSWT------AEPLEFDEVDRLVYLKAALSETLRLYPSVPEDSKHVVNDD 422

Query: 454 VLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLK--QGIFQNESPFKFTAFQ 513
           +LPDGT V AG  VTY  Y+ GRM+  WG D   FKPERW+    G F N   ++F AF 
Sbjct: 423 ILPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGKFVNHDQYRFVAFN 482

Query: 514 AGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPR 556
           AGPRIC+GKD AYLQMK   A +   ++ ++  GH+V+ +M   L M NGL V V  R
Sbjct: 483 AGPRICLGKDLAYLQMKTIAAAVLLRHRLTVAPGHKVEQKMSLTLFMKNGLLVNVHKR 511

BLAST of CsaV3_2G010790 vs. TAIR 10
Match: AT1G63710.1 (cytochrome P450, family 86, subfamily A, polypeptide 7 )

HSP 1 Score: 369.8 bits (948), Expect = 3.9e-102
Identity = 212/540 (39.26%), Postives = 318/540 (58.89%), Query Frame = 0

Query: 32  GVVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGY 91
           G  A +++ + +    W + + R        +GP+ WP+VG+      N HRMHD+I   
Sbjct: 3   GSTAAIILTLIVTYIIWFVSLRR------SYKGPRVWPLVGSLPALITNAHRMHDFIADN 62

Query: 92  LNL----YRTVVVPMPFTT-----YTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLG 151
           L +    Y+T + P+PF        T   DP N++HILKT F NYPKG  + S    LLG
Sbjct: 63  LRMCGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLG 122

Query: 152 DGIFNVDGELWRKQRKTASFEFASKNLRD-FSTLVFRDYGLKLSTILTRASLLNQQIDMQ 211
           DGIFN DG+ WR QRKTA+ EF ++ LR   +  V R    +L  IL  A    + ID+Q
Sbjct: 123 DGIFNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQ 182

Query: 212 ELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDP--LWKIKRFL 271
           ++L+R+T D+IC + FG +  +L+P  PEN FA AFD A      RFI P  +WKI+++L
Sbjct: 183 DVLLRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWL 242

Query: 272 NLGSEALLDKSIKTIDDFTYSVIATRKKEI---QEAQTTHTDPNKMKHDILSRFIELGED 331
            LG E  + +SI  +D++   +I TRK E+   Q+ ++ H        D+LSRF++  E 
Sbjct: 243 RLGLEDDMSRSISHVDNYLSEIINTRKLELLGQQQDESRH-------DDLLSRFMKKKE- 302

Query: 332 PNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKE 391
               ++DK L+ + LNF++AGRDT++  +SW  +++  +  V +K+ +E+          
Sbjct: 303 ---SYSDKYLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEI---------- 362

Query: 392 HSISLLQYQPNDIQSFDTRVVQFS-QLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGI 451
              ++L      I++ DT V +++ + L +D + +L YL A ++ETLRLYP+VP+D K +
Sbjct: 363 --CTIL------IKTRDTNVSKWTDEPLTFDEIDQLVYLKAALSETLRLYPSVPEDSKFV 422

Query: 452 LEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTA 511
           + +DVLPDGT V +G  VTY  YS+GRM++ WG D   FKPERWL++   +  + +KF A
Sbjct: 423 VANDVLPDGTFVPSGSNVTYSIYSVGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVA 482

Query: 512 FQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPR 556
           F AGPRIC+GKD AYLQMK   A +   ++ ++  GHRV+ +M   L M  GLK+ V  R
Sbjct: 483 FNAGPRICLGKDLAYLQMKSITASILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKR 507

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004138991.20.0e+00100.00cytochrome P450 704B1 [Cucumis sativus] >KGN61391.1 hypothetical protein Csa_006... [more]
XP_008441698.13.0e-30694.43PREDICTED: cytochrome P450 704B1 [Cucumis melo][more]
KAA0032319.14.4e-28195.63cytochrome P450 704B1 [Cucumis melo var. makuwa] >TYJ99426.1 cytochrome P450 704... [more]
XP_022946229.18.9e-25882.59cytochrome P450 704B1 isoform X1 [Cucurbita moschata][more]
XP_023545882.11.5e-25782.59cytochrome P450 704B1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9C7881.0e-22773.80Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1[more]
Q50EK31.1e-12847.55Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1[more]
O230663.8e-10239.03Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Q9CAD65.5e-10139.26Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
O808235.5e-10140.11Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LN460.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108690 PE=3 SV=1[more]
A0A1S3B4S21.5e-30694.43cytochrome P450 704B1 OS=Cucumis melo OX=3656 GN=LOC103485770 PE=3 SV=1[more]
A0A5D3BJX92.1e-28195.63Cytochrome P450 704B1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold31... [more]
A0A6J1G3784.3e-25882.59cytochrome P450 704B1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450376 P... [more]
A0A6J1ICQ43.7e-25782.22cytochrome P450 704B1 OS=Cucurbita maxima OX=3661 GN=LOC111471352 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G69500.17.1e-22973.80cytochrome P450, family 704, subfamily B, polypeptide 1 [more]
AT2G45510.13.5e-11143.75cytochrome P450, family 704, subfamily A, polypeptide 2 [more]
AT2G44890.17.4e-10943.40cytochrome P450, family 704, subfamily A, polypeptide 1 [more]
AT4G00360.12.7e-10339.03cytochrome P450, family 86, subfamily A, polypeptide 2 [more]
AT1G63710.13.9e-10239.26cytochrome P450, family 86, subfamily A, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 336..353
score: 42.36
coord: 416..427
score: 43.68
coord: 494..503
score: 48.47
coord: 503..514
score: 31.25
IPR001128Cytochrome P450PFAMPF00067p450coord: 64..547
e-value: 2.4E-65
score: 221.1
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 345..371
score: 23.66
coord: 415..433
score: 29.19
coord: 493..503
score: 46.58
coord: 325..342
score: 32.39
coord: 503..526
score: 21.32
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 49..556
e-value: 1.2E-99
score: 336.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 64..555
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 37..553
NoneNo IPR availablePANTHERPTHR24296:SF8CYTOCHROME P450 704B1coord: 37..553

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G010790.1CsaV3_2G010790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen