CsaV3_2G005010 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_2G005010
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionkunitz trypsin inhibitor 1-like
Locationchr2: 2715081 .. 2715786 (-)
RNA-Seq ExpressionCsaV3_2G005010
SyntenyCsaV3_2G005010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTATGTATTAAATCTGTTAGAGTGTTGATATACAACTATTAAATGTGTACCTACACTTCACTCACTATTTAAGCAGATAGATTATGATATATTGAATTCTTAACAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGAATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAA

mRNA sequence

ATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGAATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAA

Coding sequence (CDS)

ATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGAATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAA

Protein sequence

MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK*
Homology
BLAST of CsaV3_2G005010 vs. NCBI nr
Match: KAE8651587.1 (hypothetical protein Csa_023465 [Cucumis sativus])

HSP 1 Score: 422.9 bits (1086), Expect = 1.5e-114
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL
Sbjct: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
           LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG
Sbjct: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE
Sbjct: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180

Query: 181 FVRVDTGEELGSILKEKK 199
           FVRVDTGEELGSILKEKK
Sbjct: 181 FVRVDTGEELGSILKEKK 198

BLAST of CsaV3_2G005010 vs. NCBI nr
Match: XP_004139193.1 (endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus])

HSP 1 Score: 422.9 bits (1086), Expect = 1.5e-114
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL
Sbjct: 19  MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 78

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
           LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG
Sbjct: 79  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 138

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE
Sbjct: 139 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 198

Query: 181 FVRVDTGEELGSILKEKK 199
           FVRVDTGEELGSILKEKK
Sbjct: 199 FVRVDTGEELGSILKEKK 216

BLAST of CsaV3_2G005010 vs. NCBI nr
Match: KAA0063688.1 (endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >TYK18339.1 endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 386.3 bits (991), Expect = 1.5e-103
Identity = 182/199 (91.46%), Postives = 187/199 (93.97%), Query Frame = 0

Query: 1   MAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 60
           MAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP
Sbjct: 19  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 78

Query: 61  LLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKL 120
           L SQE GFGV+L PY EG+DTIREGRDLKFVFQ FTICITGTQWKVGE DPK+GRRFVKL
Sbjct: 79  LFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 138

Query: 121 GNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPF 180
           G DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGILIEKGVRFLALDGPAFPF
Sbjct: 139 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPF 198

Query: 181 EFVRVDTGEELGSILKEKK 199
           EFVRVD  EELGSIL+EKK
Sbjct: 199 EFVRVDPVEELGSILQEKK 217

BLAST of CsaV3_2G005010 vs. NCBI nr
Match: XP_008455014.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo])

HSP 1 Score: 383.6 bits (984), Expect = 1.0e-102
Identity = 181/198 (91.41%), Postives = 186/198 (93.94%), Query Frame = 0

Query: 1   MAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 60
           MAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP
Sbjct: 19  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 78

Query: 61  LLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKL 120
           LLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGTQWKVGE DPK+GRRFVKL
Sbjct: 79  LLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 138

Query: 121 GNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPF 180
           G DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGILIEKGVRFLALDGPAFPF
Sbjct: 139 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPF 198

Query: 181 EFVRVDTGEELGSILKEK 198
           EFVRVD  EELGSIL+EK
Sbjct: 199 EFVRVDPVEELGSILQEK 216

BLAST of CsaV3_2G005010 vs. NCBI nr
Match: XP_038888505.1 (kunitz type trypsin inhibitor 111-like [Benincasa hispida])

HSP 1 Score: 368.6 bits (945), Expect = 3.3e-98
Identity = 173/199 (86.93%), Postives = 184/199 (92.46%), Query Frame = 0

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MAI S AQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL
Sbjct: 18  MAISSIAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 77

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
           LSQ+IGFGVT TPYL G+DTIREGRDLK VFQAFTICIT TQWKVGE DP +GRRFVKLG
Sbjct: 78  LSQDIGFGVTFTPYLAGEDTIREGRDLKVVFQAFTICITSTQWKVGEADPITGRRFVKLG 137

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           ND+TATGYFRIDKS+LGVYNIGWCPSDVP +GRPRCGSAGILIEKG+R LALDG AFPFE
Sbjct: 138 NDDTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDGSAFPFE 197

Query: 181 FVRVD-TGEELGSILKEKK 199
           FVRV+  G++L SI +EKK
Sbjct: 198 FVRVEIAGKKLDSIFQEKK 216

BLAST of CsaV3_2G005010 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 2.6e-37
Identity = 82/184 (44.57%), Postives = 115/184 (62.50%), Query Frame = 0

Query: 5   STAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQE 64
           S AQF    V+DT G P++ D +YFI+PAIT   G+LTL+TR  + CPF VG  P   Q 
Sbjct: 22  SIAQF----VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCPFNVGLDPDAPQ- 81

Query: 65  IGFGVTLTPYLEG--DDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLGND 124
            GF V L+P++    +D +R GRDL+ +FQA T C   T+W++GE D  +GRRF+  G D
Sbjct: 82  -GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTGRRFIITGRD 141

Query: 125 NTATG----YFRIDKS-NLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAF 182
           ++  G    +FRI ++ + G++NI WCP++V    +  CG+ GI+ E G   LALDG A 
Sbjct: 142 DSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKILLALDGSAL 197

BLAST of CsaV3_2G005010 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.3e-33
Identity = 78/187 (41.71%), Postives = 103/187 (55.08%), Query Frame = 0

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MA  S AQF    V+DT G P++ D EYFI+PAIT   G  TL+T  G  CP +VG +  
Sbjct: 18  MATTSIAQF----VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVTGNG-PCPLHVG-LDN 77

Query: 61  LSQEIGFGVTLTPYL--EGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVK 120
               +G  V  TP+     DD +R  RDL+  F   T C   T W++GE+D  SGRR + 
Sbjct: 78  TEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKDATSGRRLIV 137

Query: 121 LGNDNTA---TGYFRIDKSNL-GVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDG 180
            G DN A     +FRI ++   G YNI WCP++     + +CG+ G++ E G   LALDG
Sbjct: 138 TGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRENGKNLLALDG 197

Query: 181 PAFPFEF 182
            A P  F
Sbjct: 198 DALPVVF 198

BLAST of CsaV3_2G005010 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 4.3e-32
Identity = 72/175 (41.14%), Postives = 102/175 (58.29%), Query Frame = 0

Query: 14  VLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTP 73
           VLDT G P++ D EYFI+P IT+  G  T+++R  + CP +VG + L     G  V  TP
Sbjct: 29  VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR-NESCPLHVG-LELTGLGRGLVVKFTP 88

Query: 74  YL--EGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLGNDNTATG---- 133
           +      D +R  RDL+  FQA + C+  T+W++GE+D KSGRR +  G D+   G    
Sbjct: 89  FAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLGEKDTKSGRRLIITGTDSATNGSYGN 148

Query: 134 YFRIDKSNL-GVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFEF 182
           +FRI ++ L G+YNI WCP++V    +  CG+  +L E G   LALDG   P  F
Sbjct: 149 FFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDMLNENGKILLALDGGPLPLVF 201

BLAST of CsaV3_2G005010 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.8e-15
Identity = 56/183 (30.60%), Postives = 81/183 (44.26%), Query Frame = 0

Query: 14  VLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTP 73
           V+D +G  +  +  Y++ P I    G LTL  R G  CP+ + Q      E G  V  + 
Sbjct: 30  VVDIDGNAMFHE-SYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 74  YLEGDDTIREGRDLKFVFQ-AFTICITGTQWKVGEEDPKSGRRFVKLG------NDNTAT 133
           +      + E ++L        TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 134 GYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI-EKGVRFLALDGPAFPFEFVRVD 189
            +F+I+KS    Y   +CP      G P+C   GI I E GVR LAL    F   F + +
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKAN 209

BLAST of CsaV3_2G005010 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 9.1e-14
Identity = 56/167 (33.53%), Postives = 75/167 (44.91%), Query Frame = 0

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           +AI  +     PPV DTEG  L  D  Y++ PA     G LT+  R    CP  V Q   
Sbjct: 14  LAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV-LPCPLLVAQ-ET 73

Query: 61  LSQEIGFGVTLTPY----LEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRF 120
             +  GF V  TP+       D TIR   D++  F A TIC+  T+W VG+E P +G R 
Sbjct: 74  DERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGDE-PLTGARR 133

Query: 121 VKLG-----NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGS 159
           V  G     + +     FR++K   G Y +  C       G  R G+
Sbjct: 134 VVTGPLIGPSPSGRENAFRVEKYG-GGYKLVSCRDSCQDLGVSRDGA 176

BLAST of CsaV3_2G005010 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 7.2e-115
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL
Sbjct: 19  MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 78

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
           LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG
Sbjct: 79  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 138

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE
Sbjct: 139 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 198

Query: 181 FVRVDTGEELGSILKEKK 199
           FVRVDTGEELGSILKEKK
Sbjct: 199 FVRVDTGEELGSILKEKK 216

BLAST of CsaV3_2G005010 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 7.5e-104
Identity = 182/199 (91.46%), Postives = 187/199 (93.97%), Query Frame = 0

Query: 1   MAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 60
           MAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP
Sbjct: 19  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 78

Query: 61  LLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKL 120
           L SQE GFGV+L PY EG+DTIREGRDLKFVFQ FTICITGTQWKVGE DPK+GRRFVKL
Sbjct: 79  LFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 138

Query: 121 GNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPF 180
           G DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGILIEKGVRFLALDGPAFPF
Sbjct: 139 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPF 198

Query: 181 EFVRVDTGEELGSILKEKK 199
           EFVRVD  EELGSIL+EKK
Sbjct: 199 EFVRVDPVEELGSILQEKK 217

BLAST of CsaV3_2G005010 vs. ExPASy TrEMBL
Match: A0A1S3C0M7 (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=3 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 4.9e-103
Identity = 181/198 (91.41%), Postives = 186/198 (93.94%), Query Frame = 0

Query: 1   MAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 60
           MAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP
Sbjct: 19  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 78

Query: 61  LLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKL 120
           LLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGTQWKVGE DPK+GRRFVKL
Sbjct: 79  LLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 138

Query: 121 GNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPF 180
           G DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGILIEKGVRFLALDGPAFPF
Sbjct: 139 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPF 198

Query: 181 EFVRVDTGEELGSILKEK 198
           EFVRVD  EELGSIL+EK
Sbjct: 199 EFVRVDPVEELGSILQEK 216

BLAST of CsaV3_2G005010 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 5.1e-84
Identity = 147/183 (80.33%), Postives = 162/183 (88.52%), Query Frame = 0

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL+TRKGDQCPF+VGQVPL
Sbjct: 17  MAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPL 76

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
            SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W+VGE DP +GRRFV+ G
Sbjct: 77  RSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETG 136

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           +D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILIEKG+R LALDG AFPFE
Sbjct: 137 DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFE 196

Query: 181 FVR 184
           FVR
Sbjct: 197 FVR 197

BLAST of CsaV3_2G005010 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.1e-81
Identity = 144/183 (78.69%), Postives = 158/183 (86.34%), Query Frame = 0

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MA  S AQ   PPVLD EGRPL+ +VEYFIKPAITDVAGNLTL+TRKGDQCPF+VGQVPL
Sbjct: 17  MAASSIAQ--SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPL 76

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
            SQEIGFGVT TPYL  +D IREGRDLK VFQAF+ICIT T W+VGE DP +GRRFV+ G
Sbjct: 77  RSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADPTTGRRFVETG 136

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           +D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILIEKG+R LALDG AFPFE
Sbjct: 137 DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFE 196

Query: 181 FVR 184
           FVR
Sbjct: 197 FVR 197

BLAST of CsaV3_2G005010 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 82.8 bits (203), Expect = 3.4e-16
Identity = 56/183 (30.60%), Postives = 81/183 (44.26%), Query Frame = 0

Query: 14  VLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTP 73
           V+D +G  +  +  Y++ P I    G LTL  R G  CP+ + Q      E G  V  + 
Sbjct: 30  VVDIDGNAMFHE-SYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 74  YLEGDDTIREGRDLKFVFQ-AFTICITGTQWKVGEEDPKSGRRFVKLG------NDNTAT 133
           +      + E ++L        TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 134 GYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI-EKGVRFLALDGPAFPFEFVRVD 189
            +F+I+KS    Y   +CP      G P+C   GI I E GVR LAL    F   F + +
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKAN 209

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8651587.11.5e-114100.00hypothetical protein Csa_023465 [Cucumis sativus][more]
XP_004139193.11.5e-114100.00endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus][more]
KAA0063688.11.5e-10391.46endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >T... [more]
XP_008455014.11.0e-10291.41PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo][more]
XP_038888505.13.3e-9886.93kunitz type trypsin inhibitor 111-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
G7LCV72.6e-3744.57Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ... [more]
A0A072TH682.3e-3341.71Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ... [more]
G7LCV14.3e-3241.14Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ... [more]
Q8RXD54.8e-1530.60Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1[more]
P294219.1e-1433.53Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R... [more]
Match NameE-valueIdentityDescription
A0A0A0LGB47.2e-115100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1[more]
A0A5A7V7Y27.5e-10491.46Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa O... [more]
A0A1S3C0M74.9e-10391.41endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1FD705.1e-8480.33alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1HLP63.1e-8178.69kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G73260.13.4e-1630.60kunitz trypsin inhibitor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 13..185
e-value: 8.2E-27
score: 105.1
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 14..182
e-value: 7.2E-36
score: 123.7
IPR002160Proteinase inhibitor I3, Kunitz legumePANTHERPTHR33107KUNITZ TRYPSIN INHIBITOR 2coord: 1..184
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 14..30
NoneNo IPR availableGENE3D2.80.10.50coord: 11..186
e-value: 2.9E-38
score: 133.3
NoneNo IPR availablePANTHERPTHR33107:SF51PUTATIVE-RELATEDcoord: 1..184
IPR011065Kunitz inhibitor STI-like superfamilySUPERFAMILY50386STI-likecoord: 11..185

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G005010.1CsaV3_2G005010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004866 endopeptidase inhibitor activity