CsaV3_2G002740 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_2G002740
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	NADH-cytochrome b5 reductase
Location	chr2: 1225123 .. 1229978 (+)
RNA-Seq Expression	CsaV3_2G002740
Synteny	CsaV3_2G002740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAAACTGGAGAAGCAAAGGCTTTATTCCAAAGAATAAAAAAAAACCCAAGAGAGGTTTTGTTAAATCACTCATAAATTTCGTTACACATTTCGTCTTTTTCTTCTTCTTGATTTCTTTTCTCCGGTGATCATTCATGGCGACATTCCTCAGAAGATTAGCAAACGCTGCTCCATCACTACGCTACAATGCATTATTAGGAACCCAATCAAAGATCCATTCCTCTAACTTCAGATTCCCCATTGGAGCCATCGCTGCAGCTTCCGGTGGAATATCGTATTTGTATTATGTTTCCTATTCGGATTTGGTAATTCACCTCAATTTATTCTCTTCTCTATTGAATGTGGTTGCTTGTATTGAATTCTTCTTTATTTTTCATAATTTTGAACTCTTTGTTATATGGGTATGGATTGGTTTGTCAATTGTCAGTTAGCTTCATTTTCGTCAGCTGATTGTATGATTCGGTTTTATCTTCTATTTGCAACTTTATTTGCAGGTTCATCTAGATCAAAGCAGTGAAGAGGCAAGCCCAAAAGTTGGTCAGTTTGTCTTATTTTCTCTTTCTTTAACAACTTTTGTAACAAAAGAAAGAGAATATTTAGCCCGAGTCAACTTTTAAATTTGAATATCGAGTTTTAGTGGGAGTGAATCGTGCAAAATTGATAATGATCTTTAATTTTACACTTTTGAATGTAATTTCTTTATCATCAAGATTAATTTTGAATGATTAAAAGTGTGTTTGGAAGTGATTGTACCAAGTTTAGAATCACTCTAGAACATGCCTCAGTAAATCTTAATTCATAAAAAATGGTTTGTTTGCATTTCCAGCACTCAAGTCAGGTAAATGGATTGAGTTCAAGTTACAAGACTCTGCACGGGTTACTCATAATACTCACTTATTCAGGTACAAAATTTACGATATATGTTTTATAGTGTACTTTAGGAAGCTAATGAAGCATTTAATTGTCTGTATTACCTTGTCAATACTATATAGTACCTGGTAGACATATCTCTAATTTTAGATTTCAGGTAAGAACTCGTTGTGGGGTTAGCAGAAAACAATTACTATAACTACTGCCCAAACTTACCCCTTGAAATAAAGAACGGAGGCCACTATTCTAATTACATTTCATACCACTACTATTTCTGTTTCTTTTTCCCAGCTCCATAACATAACCTCACTCAAGTCCTGTGTGCAGTAAATGCAACTAATTAAAATCAGGTGCTTTTCTTGCCATCTTTTTACATATGCAATATTTGCTAACACACAAGTTGGGCAGATTTTCATTTGATCCTAGTGAAACATTGGGGTTAAATGTCGCGTCATGCATCTATACAAGGTATGCTTTTCTCTTTCCTTCTGTCCTCCTCGACCCTGCTTTTTCAATTAAACCATTGGAGATGTATTTTTCTTTGACTGATTGCTAAATTAAAAGGAATGTGCTTCTTGGTTATTCATTACGGTGTATTGTTGTAACCACAGAGCTCCAATTGGACAAGACGATGAAGGGAAAGCAAAATACGTTGTGCGCCCGTAAGTTAACTTGTTCAAACATTGGGATGCTTCATTTTAATTTATCTATATTTGATTATCGAAAATTTGGTAGAGGGTTCGGCATAATTATAAAGGATTTTATAATTCCACCAGTTGATTATGAATTCAAAATACTCGCATCCTTGGGAAGTTGAGATGTCCAGCCAGTCCATTCCATGTTGATGACAAACAATATTCACAGTTTCAATCTTTATGATATTCCAGCTATTTTTGCTTCCTGCTTGTTCACCATCAATCCAACCCCTTTACATTATGTTACTTGGTCTTCATGAAGTTATTTTATTATCCAGTTTATATTAATTTGTTTTTGAAACATCAAGATGAGAGTCAATTGGATTTACTGATGTATGTAATGTGAAGCTCTGTATATTCATTTTTCTTTCCTTTATTTTAAATTAATGATTTTTGTCCAGGTATACACCTATATCAGATCCTGATGCAAAGGGATACTTTGATTTACTAATTAAGGTATATTTTTGTTCACCTGACATTCTATATTTCCTCATCGGATATCTACTTATCCTTTTGAAGACAATTTTTTTATAATGAGGTGTGATATGTTTTACATAGATTGTGCTTTCTTTGATGAATTTTGTAGGTATATCCTCAAGGAAAAATGAGTCAACATTTTGCTACCTTGAAACCCGGGGATGTACTTGAAGTGAAAGGGTATCAATTTTTTTTTTCTTCCTTGTTATCTTCAAGTCATGTATTGTATATCGTAACTCTTTTCCCTTCATTGCTGTGCAGCCCCATTGAAAAGCTCAAATACTCTCCAAATATGAAGAAACACATTGGCATGGTAAGAAGAATCGTGTTGGATTGCTCGTGATAATATCAATTATAAATATATAACTAAATTGCTTTGCAGCCCGATTAAAAAGCTCAGATACTTCTACTTGGCATATCATTGTTTTTTGCATTGGTAATGCTGTTGCCATTACTGCCATTTAATTTAAAGAGATAAAAGTTCAAGGTTTCATTTAATAAATTGTATATTTTAATTGACAAGAAAGTCACGTAGCTTTCTTTGGAATGAGATTGACCATTTCTATTATATGCACGGGAAAAGGAAATGCAGCTTAACTGAAATTAGAGTAACTAAATACTGAAATCAACTATACGTCATATCTCCAAATTTTGTTTTCTGCCCTTGCAGATTGCTGGTGGCACAGGAATTACTCCCATGCTCCAGGTTATTGATGCTATAGTAAAAAATCCAGATGACAATACCCAGGTGCGCAATGATCTTTTCTTGCAAAATTTCTGAACTATGCCAAAACTGACCAGTAAAGTTGAAATCCAATGTTCCTTTATTTGCACTTTGATTAGATTTTGCAAGAATGTTTCTTTGTTCAATCACTTCGTAAAAAATGTTTTATTCATTTAATGAAAACTTCCATTGGAAAACCTAACTCTCCATCTATCCCTTATTTGGAGCTCGTGAAAATGCACTCCAATTGGGACTGTTGTAGTATAAAAGTAAACTTTCGGCATTTCCTATGGACATTGAAGCTGCATTTTATTCTGACACTTTAATGTATGAACTTGGGAAAATATTTATGATAAGAACAAAATTACCTCAGCTATAACTTTCAATGATTGCTTGAGCATGTCCTTTAATACTTACAGGTCTCGCTGCTTTACGGGAATGTCTCTCCTGATGACATATTGCTTAAACAGAAACTTGACATACTTGCATCTAGCCACCCAAATATAAAGGTATTATTTGTTTCCGTAATACAAAATTGCTGTAGTATTTAGAAGTTGATTTCCTATCAAATGATCGTGAGATGGACAATGGCATATTAAGAACCCAGTTTCAGCAGCGTGTTCATGTATGAAGTGTACTCAATGTTATGTGAGATGTGTTACACATGCAAATCATGAAATTTTGACTTACTACGAAGACGTTAGTGAAATAGAATATGAGAAACTGGACAAAAAAGAAAAGAATTTAATGAACATAACATAAAAATTGGAAAAGAAGGATAAACTTGTTTGTGTATCGATCTTGACATTTACCGCGAGAAATGATTTAACTATTTCCCCTTTTTTGCATGCCTGTGCAATATAATTTATTTTCCTGCCAGGTGCAGTTTTTTTTACCGTCGCTTTGATTCTATAGTTTCCTTTTCTTTTGGTTACATCTTAGGTGTAAATGCTCCCCCACTTCCATTAACATAGACATAACTCTTTGATATCCAATTGGAGAGGCTTTTTTGTAACTCTCGTGAATTAGAGATCCCCCCCCTTGGCCTCTTGAAATTTCATTCAATCAATAAATTTGTATTTATATCATATGTGTGTGTGTGTGTATGTGTATATATCCCTGCCTCTTAATGAAAGTGGTGAACTCTATGGTAACAAAAATTTAGGTATTCTACACGGTTGATAAACCAACAAAGAATTGGAAGGGAGGAAAAGGATACGTATCAAAGGATATGGTCATCAAAGGCTTACCACCCCCAGGGGATGACGCACTAATCCTCGTAAGTGTTTATTGTTGTTGCTGCTGTAGCAATACTTCATCTCTTTCTCTTTGTTAACGTGTTTATCATTTCTTACCCAAACTAAAATAATATGCACCACAAACATAGACTATTATAAATTAGAGGGTTTTTGTTGTACTACCATGGTCTATTCTAGATACATAACGAACTTGGTTCCATGCGTTCAGGTATGCGGTCCACCAGGGATGATGAAACACATTTCTGGGGACAAAGCTCCCGATTACTCACAGGGAGAGGTAATATTTATTTAGGATTTTCAGTGTCTTACCGTGAACTTAAATTGATTTATCAACTGTTAATACATCTTAATTTACATCTTTTTTACTTCTTTCTTGCAGCTTGATGGCATGCTAAAGGAATTGGGATATACGAAGGAAATGGTTTACAAGTTTTAACAAGATTCACAAAAGTTTTAGCTGTTGACGGACGTATCAACTTAATGTTAGGGAGTGTTTTCTGAAGCCTTGTTAGAATTATTGTCATTGGACCAAATTTTAAATCAAAATAATTTCCAAGTTGGTGTTCGTTATATAAGTTGATCTCTTGAAAGCCAGTTTATGTTGCTTCTTCTCGTACTTTTTTATGTCTTGATAACGAGGCTGGTATCTTTTCTCAATATTCCACAATATGACGATATATTCGTAAATTAGAGCGTTCGACATGGATAGCTTCTATTTGGCAAGCAAAAGAATCAAAACTTGTGGAAATATCCAAGGAAGGAATTCACACATTTATGAAGTTATTGGTCAAAAATACATAAATAGAATCTCAATACTTTACCCTTTTTG

mRNA sequence

ATGGCGACATTCCTCAGAAGATTAGCAAACGCTGCTCCATCACTACGCTACAATGCATTATTAGGAACCCAATCAAAGATCCATTCCTCTAACTTCAGATTCCCCATTGGAGCCATCGCTGCAGCTTCCGGTGGAATATCGTATTTGTATTATGTTTCCTATTCGGATTTGGTTCATCTAGATCAAAGCAGTGAAGAGGCAAGCCCAAAAGTTGCACTCAAGTCAGGTAAATGGATTGAGTTCAAGTTACAAGACTCTGCACGGGTTACTCATAATACTCACTTATTCAGATTTTCATTTGATCCTAGTGAAACATTGGGGTTAAATGTCGCGTCATGCATCTATACAAGAGCTCCAATTGGACAAGACGATGAAGGGAAAGCAAAATACGTTGTGCGCCCGTATACACCTATATCAGATCCTGATGCAAAGGGATACTTTGATTTACTAATTAAGGTATATCCTCAAGGAAAAATGAGTCAACATTTTGCTACCTTGAAACCCGGGGATGTACTTGAAGTGAAAGGCCCCATTGAAAAGCTCAAATACTCTCCAAATATGAAGAAACACATTGGCATGATTGCTGGTGGCACAGGAATTACTCCCATGCTCCAGGTTATTGATGCTATAGTAAAAAATCCAGATGACAATACCCAGGTCTCGCTGCTTTACGGGAATGTCTCTCCTGATGACATATTGCTTAAACAGAAACTTGACATACTTGCATCTAGCCACCCAAATATAAAGGTATTCTACACGGTTGATAAACCAACAAAGAATTGGAAGGGAGGAAAAGGATACGTATCAAAGGATATGGTCATCAAAGGCTTACCACCCCCAGGGGATGACGCACTAATCCTCGTATGCGGTCCACCAGGGATGATGAAACACATTTCTGGGGACAAAGCTCCCGATTACTCACAGGGAGAGCTTGATGGCATGCTAAAGGAATTGGGATATACGAAGGAAATGGTTTACAAGTTTTAA

Coding sequence (CDS)

ATGGCGACATTCCTCAGAAGATTAGCAAACGCTGCTCCATCACTACGCTACAATGCATTATTAGGAACCCAATCAAAGATCCATTCCTCTAACTTCAGATTCCCCATTGGAGCCATCGCTGCAGCTTCCGGTGGAATATCGTATTTGTATTATGTTTCCTATTCGGATTTGGTTCATCTAGATCAAAGCAGTGAAGAGGCAAGCCCAAAAGTTGCACTCAAGTCAGGTAAATGGATTGAGTTCAAGTTACAAGACTCTGCACGGGTTACTCATAATACTCACTTATTCAGATTTTCATTTGATCCTAGTGAAACATTGGGGTTAAATGTCGCGTCATGCATCTATACAAGAGCTCCAATTGGACAAGACGATGAAGGGAAAGCAAAATACGTTGTGCGCCCGTATACACCTATATCAGATCCTGATGCAAAGGGATACTTTGATTTACTAATTAAGGTATATCCTCAAGGAAAAATGAGTCAACATTTTGCTACCTTGAAACCCGGGGATGTACTTGAAGTGAAAGGCCCCATTGAAAAGCTCAAATACTCTCCAAATATGAAGAAACACATTGGCATGATTGCTGGTGGCACAGGAATTACTCCCATGCTCCAGGTTATTGATGCTATAGTAAAAAATCCAGATGACAATACCCAGGTCTCGCTGCTTTACGGGAATGTCTCTCCTGATGACATATTGCTTAAACAGAAACTTGACATACTTGCATCTAGCCACCCAAATATAAAGGTATTCTACACGGTTGATAAACCAACAAAGAATTGGAAGGGAGGAAAAGGATACGTATCAAAGGATATGGTCATCAAAGGCTTACCACCCCCAGGGGATGACGCACTAATCCTCGTATGCGGTCCACCAGGGATGATGAAACACATTTCTGGGGACAAAGCTCCCGATTACTCACAGGGAGAGCTTGATGGCATGCTAAAGGAATTGGGATATACGAAGGAAATGGTTTACAAGTTTTAA

Protein sequence

MATFLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHLDQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAPIGQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEKLKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDILASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISGDKAPDYSQGELDGMLKELGYTKEMVYKF*

Homology

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BLAST of CsaV3_2G002740 vs. NCBI nr
Match: XP_004139319.1 (NADH-cytochrome b5 reductase-like protein [Cucumis sativus] >KAE8651527.1 hypothetical protein Csa_019300 [Cucumis sativus])

HSP 1 Score: 670.2 bits (1728), Expect = 8.9e-189
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MATFLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHL 60
           MATFLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHL
Sbjct: 1   MATFLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHL 60

Query: 61  DQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAPI 120
           DQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAPI
Sbjct: 61  DQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAPI 120

Query: 121 GQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEK 180
           GQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEK
Sbjct: 121 GQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEK 180

Query: 181 LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI 240
           LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI
Sbjct: 181 LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI 240

Query: 241 LASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG 300
           LASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG
Sbjct: 241 LASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG 300

Query: 301 DKAPDYSQGELDGMLKELGYTKEMVYKF 329
           DKAPDYSQGELDGMLKELGYTKEMVYKF
Sbjct: 301 DKAPDYSQGELDGMLKELGYTKEMVYKF 328

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BLAST of CsaV3_2G002740 vs. NCBI nr
Match: XP_008457751.1 (PREDICTED: NADH-cytochrome b5 reductase-like protein [Cucumis melo] >KAA0061593.1 NADH-cytochrome b5 reductase-like protein [Cucumis melo var. makuwa] >TYJ97802.1 NADH-cytochrome b5 reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 635.6 bits (1638), Expect = 2.4e-178
Identity = 311/329 (94.53%), Postives = 320/329 (97.26%), Query Frame = 0


Query: 1   MATFLRRLANAAPS-LRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVH 60
           MATF RRLANAAPS LRYNALLGTQSKIHSSNFRFPIGAIAAASGG+SYLYYVS SD+VH
Sbjct: 1   MATFFRRLANAAPSLLRYNALLGTQSKIHSSNFRFPIGAIAAASGGVSYLYYVSSSDMVH 60

Query: 61  LDQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAP 120
           LD +SEEAS KVALKSGKWIEFKLQDSARVTHNT LFRFSFDPSETLGLNVASCIYTRAP
Sbjct: 61  LDPNSEEASSKVALKSGKWIEFKLQDSARVTHNTQLFRFSFDPSETLGLNVASCIYTRAP 120

Query: 121 IGQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIE 180
           IGQDDEGK KYVVRPYTPISDPDA+GYFDLLIKVYPQGKMSQHFA+LKPGDVLEVKGPIE
Sbjct: 121 IGQDDEGKVKYVVRPYTPISDPDARGYFDLLIKVYPQGKMSQHFASLKPGDVLEVKGPIE 180

Query: 181 KLKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD 240
           KLKYSPNMKK IGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD
Sbjct: 181 KLKYSPNMKKRIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD 240

Query: 241 ILASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHIS 300
           ILASSHPN+KVFYTVD P++NWKGGKGYVSKDMVIKGLPPPGDD+LILVCGPPGMMKHIS
Sbjct: 241 ILASSHPNLKVFYTVDNPSQNWKGGKGYVSKDMVIKGLPPPGDDSLILVCGPPGMMKHIS 300

Query: 301 GDKAPDYSQGELDGMLKELGYTKEMVYKF 329
           GDKAPDYSQGELDGMLKELGYTKEMVYKF
Sbjct: 301 GDKAPDYSQGELDGMLKELGYTKEMVYKF 329

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BLAST of CsaV3_2G002740 vs. NCBI nr
Match: XP_038889566.1 (NADH-cytochrome b5 reductase-like protein [Benincasa hispida])

HSP 1 Score: 622.9 bits (1605), Expect = 1.6e-174
Identity = 306/328 (93.29%), Postives = 315/328 (96.04%), Query Frame = 0


Query: 1   MATFLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHL 60
           MATF RRLANAAPSLRYNA LG+QSKIHSS FRFPIGAIAA SGGISYLYYVS SDLVHL
Sbjct: 1   MATFFRRLANAAPSLRYNA-LGSQSKIHSSMFRFPIGAIAAISGGISYLYYVSTSDLVHL 60

Query: 61  DQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAPI 120
           DQSSEEA PKVALKSGKWIEFKLQDSARVTH+T LFRFSFDP+E LGLNVASCIYTRAP 
Sbjct: 61  DQSSEEAGPKVALKSGKWIEFKLQDSARVTHDTQLFRFSFDPTEKLGLNVASCIYTRAPS 120

Query: 121 GQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEK 180
           GQDDEGK KYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFA+LKPGD +EVKGPIEK
Sbjct: 121 GQDDEGKVKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFASLKPGDTIEVKGPIEK 180

Query: 181 LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI 240
           LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI
Sbjct: 181 LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI 240

Query: 241 LASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG 300
           LASSHPNIKVFYTVD P+K+WKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG
Sbjct: 241 LASSHPNIKVFYTVDNPSKSWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG 300

Query: 301 DKAPDYSQGELDGMLKELGYTKEMVYKF 329
           +KAPDYSQGEL G+LKELGYTKEMVYKF
Sbjct: 301 EKAPDYSQGELVGILKELGYTKEMVYKF 327

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BLAST of CsaV3_2G002740 vs. NCBI nr
Match: XP_023547148.1 (NADH-cytochrome b5 reductase-like protein [Cucurbita pepo subsp. pepo] >XP_023547149.1 NADH-cytochrome b5 reductase-like protein [Cucurbita pepo subsp. pepo] >XP_023547151.1 NADH-cytochrome b5 reductase-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 579.3 bits (1492), Expect = 2.1e-161
Identity = 280/328 (85.37%), Postives = 303/328 (92.38%), Query Frame = 0


Query: 1   MATFLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHL 60
           MATFLRRL NAAP+LR NA  G QSKIHSS +RFPIGAIAA SGGISY+YYVS SDLVHL
Sbjct: 1   MATFLRRLVNAAPALRSNA-FGGQSKIHSSKYRFPIGAIAAVSGGISYMYYVSSSDLVHL 60

Query: 61  DQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAPI 120
           DQ+SE   PKVALKS KWIEFKLQD+ARVTH+T LFRFSFDP+E LGL+VASC+ TRAP 
Sbjct: 61  DQNSEAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGLHVASCLLTRAPS 120

Query: 121 GQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEK 180
           G D EGK KYVVRPYTPISDPD+KGYFDLLIKVYPQGKMSQHFA+LKPGDV+EVKGPIEK
Sbjct: 121 GVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPQGKMSQHFASLKPGDVVEVKGPIEK 180

Query: 181 LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI 240
           ++YSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSL+YGNVSPDDILLKQKLD+
Sbjct: 181 IRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNVSPDDILLKQKLDL 240

Query: 241 LASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG 300
           LASSHPN+KVFYTVD P+K+WKGGKGYVSKDM +KGLP PGDD LILVCGPPGMMKHISG
Sbjct: 241 LASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAVKGLPAPGDDTLILVCGPPGMMKHISG 300

Query: 301 DKAPDYSQGELDGMLKELGYTKEMVYKF 329
           +KA DYSQGEL G+L+ELGYTKEMVYKF
Sbjct: 301 EKAKDYSQGELAGILQELGYTKEMVYKF 327

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BLAST of CsaV3_2G002740 vs. NCBI nr
Match: XP_022999370.1 (NADH-cytochrome b5 reductase-like protein [Cucurbita maxima] >XP_022999371.1 NADH-cytochrome b5 reductase-like protein [Cucurbita maxima] >XP_022999372.1 NADH-cytochrome b5 reductase-like protein [Cucurbita maxima])

HSP 1 Score: 576.6 bits (1485), Expect = 1.3e-160
Identity = 279/328 (85.06%), Postives = 301/328 (91.77%), Query Frame = 0


Query: 1   MATFLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHL 60
           MATFLRRL NAAP+LR NA  G QSKIHSS +RFPIGAIAA SGGISY+YYVS SDLVHL
Sbjct: 1   MATFLRRLVNAAPALRSNA-FGGQSKIHSSKYRFPIGAIAAVSGGISYMYYVSSSDLVHL 60

Query: 61  DQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAPI 120
           DQ SE   PKVALKS KWIEFKLQD+ARVTH+T LFRFSFDP+E LGL+VASC+ TRAP 
Sbjct: 61  DQKSEAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGLHVASCLLTRAPS 120

Query: 121 GQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEK 180
           G D EGK KYVVRPYTPISDPD+KGYFDLLIKVYPQGKMSQHFA+LKPGDV+EVKGPIEK
Sbjct: 121 GVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPQGKMSQHFASLKPGDVVEVKGPIEK 180

Query: 181 LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI 240
           ++YSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSL+YGNVSPDDILLKQKLD+
Sbjct: 181 IRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNVSPDDILLKQKLDL 240

Query: 241 LASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG 300
           LASSHPN+KVFYTVD P K+WKGGKGYV+KDM IKGLP PGDD LILVCGPPGMMKHISG
Sbjct: 241 LASSHPNLKVFYTVDNPPKSWKGGKGYVTKDMAIKGLPAPGDDTLILVCGPPGMMKHISG 300

Query: 301 DKAPDYSQGELDGMLKELGYTKEMVYKF 329
           +KA DYSQGE+ G+L+ELGYTKEMVYKF
Sbjct: 301 EKAKDYSQGEIAGILQELGYTKEMVYKF 327

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BLAST of CsaV3_2G002740 vs. ExPASy Swiss-Prot
Match: P83291 (NADH-cytochrome b5 reductase-like protein OS=Arabidopsis thaliana OX=3702 GN=CBR2 PE=1 SV=2)

HSP 1 Score: 515.4 bits (1326), Expect = 4.8e-145
Identity = 247/329 (75.08%), Postives = 282/329 (85.71%), Query Frame = 0


Query: 1   MAT-FLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVH 60
           MAT F RRLA +AP + +    G+QSK  S  FRF  GAIAA SGG SY Y  S ++LV+
Sbjct: 1   MATSFFRRLARSAP-ITFPVAFGSQSKSGSGAFRFSTGAIAALSGGFSYYYLTSGNNLVY 60

Query: 61  LDQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAP 120
           LDQ+ EE  PK AL   KW+EFKLQD+ARV+HNT LFRFSFDPS  LGL+VASC+ TRAP
Sbjct: 61  LDQAKEETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSFDPSAELGLHVASCLLTRAP 120

Query: 121 IGQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIE 180
           +G + EGK KYV+RPYTPISDP+AKGYFDLLIKVYP GKMSQHFA+LKPGDVLEVKGP+E
Sbjct: 121 LGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPGDVLEVKGPVE 180

Query: 181 KLKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD 240
           K KYSPNMKKHIGMIAGG+GITPMLQVIDAIVKNP+DNTQ+SLLY NVSPDDILLKQKLD
Sbjct: 181 KFKYSPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPDDILLKQKLD 240

Query: 241 ILASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHIS 300
           +L ++HPN+K+FYTVD PTKNWKGG GY+SKDM +KGLP P DD LILVCGPPGMM+HIS
Sbjct: 241 VLQANHPNLKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVCGPPGMMEHIS 300

Query: 301 GDKAPDYSQGELDGMLKELGYTKEMVYKF 329
           G KAPD+SQGE+ G+LKELGYT+EMV+KF
Sbjct: 301 GGKAPDWSQGEVKGILKELGYTEEMVFKF 328

[Close]

BLAST of CsaV3_2G002740 vs. ExPASy Swiss-Prot
Match: A1CRK9 (NADH-cytochrome b5 reductase 2 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=mcr1 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.5e-66
Identity = 129/254 (50.79%), Postives = 166/254 (65.35%), Query Frame = 0


Query: 78  WIEFKLQDSARVTHNTHLFRFSFDPSETL-GLNVASCIYTRAPIGQDDEGKAKYVVRPYT 137
           W++ KL +   ++HNT   RF F   E + GL VAS + T+    +  EGK   V+RPYT
Sbjct: 74  WVDLKLSEIENLSHNTKRLRFEFADKEAVSGLQVASALLTKF---KPAEGKP--VIRPYT 133

Query: 138 PISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEKLKYSPNMKKHIGMIAG 197
           P+SD D  GY DL++KVYP G MS+H  ++     LE KGPI K  +  N  KHI +IAG
Sbjct: 134 PVSDEDQPGYLDLVVKVYPNGPMSEHLHSMNVDQRLEFKGPIPKYPWETNKHKHICLIAG 193

Query: 198 GTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDILASSHP-NIKVFYTVD 257
           GTGITPM Q+   I KNP+D T+V+L++GNVS +DILLK++L  L ++HP   K FY +D
Sbjct: 194 GTGITPMYQLARQIFKNPEDQTKVTLVFGNVSEEDILLKKELQELENTHPRRFKAFYVLD 253

Query: 258 KPTKNWKGGKGYVSKDMVIKGLP-PPGDDALILVCGPPGMMKHISGDKAPDYSQGELDGM 317
            P K W GGKGYV+K+++   LP P  +D  I VCGPPGM K ISG K     QGEL G+
Sbjct: 254 NPPKEWTGGKGYVTKELLKTVLPEPKEEDIKIFVCGPPGMYKAISGPKVSPKDQGELTGL 313

Query: 318 LKELGYTKEMVYKF 329
           L ELGY K+ VYKF
Sbjct: 314 LAELGYNKDQVYKF 322

[Close]

BLAST of CsaV3_2G002740 vs. ExPASy Swiss-Prot
Match: Q2UKB8 (NADH-cytochrome b5 reductase 2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mcr1 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.6e-66
Identity = 123/255 (48.24%), Postives = 170/255 (66.67%), Query Frame = 0


Query: 78  WIEFKLQDSARVTHNTHLFRFSFDPSETL-GLNVASCIYTR-APIGQDDEGKAKYVVRPY 137
           W++ KL +   ++HNT   RF F+  E + G+ +AS + T+  P+G +     K V+RPY
Sbjct: 74  WVDLKLSEIEVLSHNTKRLRFEFEDKEAVSGVTIASALLTKFKPVGAE-----KAVLRPY 133

Query: 138 TPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEKLKYSPNMKKHIGMIA 197
           TP SD D  GY DL++KVYP G MS+H  ++     L  KGP+ K ++  N  +HI +IA
Sbjct: 134 TPTSDEDQPGYLDLVVKVYPNGPMSEHLHSMNVDQRLSFKGPLPKYQWETNKHEHIALIA 193

Query: 198 GGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDILASSHP-NIKVFYTV 257
           GGTGITPM Q+I  I KNPDD T+V+L+YGNV+ DDILLK++L  L +++P   K FY +
Sbjct: 194 GGTGITPMYQLIRQIFKNPDDKTKVTLVYGNVTEDDILLKKELQDLENTYPQRFKAFYLL 253

Query: 258 DKPTKNWKGGKGYVSKDMVIKGLPPPGDD-ALILVCGPPGMMKHISGDKAPDYSQGELDG 317
           DKP K W GGKGY++K+++   LP P ++   I VCGPPG+   +SG+K     QGEL G
Sbjct: 254 DKPPKEWTGGKGYINKELLKTVLPEPKEENQKIFVCGPPGLYNAVSGNKVSPKDQGELSG 313

Query: 318 MLKELGYTKEMVYKF 329
           +LKELGY K+ VYKF
Sbjct: 314 ILKELGYNKDQVYKF 323

[Close]

BLAST of CsaV3_2G002740 vs. ExPASy Swiss-Prot
Match: Q2HG02 (NADH-cytochrome b5 reductase 2 OS=Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) OX=306901 GN=MCR1 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.4e-66
Identity = 143/341 (41.94%), Postives = 200/341 (58.65%), Query Frame = 0


Query: 5   LRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHLDQSS 64
           +RR A   PS   +A  G  + ++        GA AAA  G  Y +          ++  
Sbjct: 23  IRRYATEPPSA--DAKKGNNTLLY--------GAAAAAVAGAGYYFLGGTPAAKKAEEKV 82

Query: 65  EEASP-----------KVALKSGK--WIEFKLQDSARVTHNTHLFRFSFDPSETL-GLNV 124
           ++AS            K AL  G+  W+  KL++   V HN+   RF     + + G++V
Sbjct: 83  KDASSAAAGKLSTSEVKQALTGGEQGWVSLKLEEVEIVNHNSKRLRFRLPEDDMVSGVHV 142

Query: 125 ASCIYTR-APIGQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPG 184
           AS I T+  P+  +     K V+RPYTP +D DA+GY DLL+K YP G MS H   + PG
Sbjct: 143 ASAILTKFKPVDAE-----KPVIRPYTPTNDEDARGYLDLLVKKYPNGPMSTHLHDMVPG 202

Query: 185 DVLEVKGPIEKLKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSP 244
             L+VKGP+ K  ++ N   HI ++AGGTGITPM Q+  AI  NPDD T+V+L++GNV  
Sbjct: 203 QRLDVKGPLPKYPWTANKHGHIALVAGGTGITPMFQLCRAIFNNPDDQTKVTLVFGNVRE 262

Query: 245 DDILLKQKLDILASSHP-NIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDAL-IL 304
           DDILLK++L  L +++P   + FY +D P K+W GGKG+++KD++   LP P D+ + + 
Sbjct: 263 DDILLKKELAALENNNPRRFRAFYVLDDPPKHWTGGKGFITKDLLKTVLPEPKDENIKVF 322

Query: 305 VCGPPGMMKHISGDKAPDYSQGELDGMLKELGYTKEMVYKF 329
           VCGPPGMM  ISG+K     QGEL G+LKELGY+ E VYKF
Sbjct: 323 VCGPPGMMDAISGNKKSPKDQGELKGILKELGYSPEQVYKF 348

[Close]

BLAST of CsaV3_2G002740 vs. ExPASy Swiss-Prot
Match: Q0CRD8 (NADH-cytochrome b5 reductase 2 OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mcr1 PE=3 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 7.6e-66
Identity = 125/254 (49.21%), Postives = 172/254 (67.72%), Query Frame = 0


Query: 78  WIEFKLQDSARVTHNTHLFRFSFDPSETL-GLNVASCIYTRAPIGQDDEGKAKYVVRPYT 137
           W++ KL +   + HNT  FRF F+  E + GLNVAS + T+    + + GKA  V+RPYT
Sbjct: 71  WVDLKLSEIEVLNHNTKRFRFEFEDKEAVSGLNVASALLTKF---KPEGGKA--VLRPYT 130

Query: 138 PISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEKLKYSPNMKKHIGMIAG 197
           P+SD    G+ DL++KVYP G MS+H  ++     LE KGP+ K  +  N  +HI +IAG
Sbjct: 131 PVSDESQPGFLDLVVKVYPNGPMSEHLHSMNVDQRLEFKGPLPKYPWEANKHQHICLIAG 190

Query: 198 GTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDILASSHP-NIKVFYTVD 257
           GTGITPM Q+   I KNP+D T+V+L+YGNVS  DILLK++L+ L +++P   K FY +D
Sbjct: 191 GTGITPMYQLARHIFKNPEDKTKVTLVYGNVSEQDILLKKELEELENTYPQRFKAFYVLD 250

Query: 258 KPTKNWKGGKGYVSKDMVIKGLPPPGDDAL-ILVCGPPGMMKHISGDKAPDYSQGELDGM 317
            P K W GGKGY+SK+++   LP P ++ + I VCGPPG+ K ISG+K     QGEL G+
Sbjct: 251 NPPKEWTGGKGYISKELLKTVLPEPKEENIKIFVCGPPGLYKAISGNKVSPKDQGELTGI 310

Query: 318 LKELGYTKEMVYKF 329
           LKELGY++E V+KF
Sbjct: 311 LKELGYSQEQVFKF 319

[Close]

BLAST of CsaV3_2G002740 vs. ExPASy TrEMBL
Match: A0A5A7V0C8 (NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00120 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.2e-178
Identity = 311/329 (94.53%), Postives = 320/329 (97.26%), Query Frame = 0


Query: 1   MATFLRRLANAAPS-LRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVH 60
           MATF RRLANAAPS LRYNALLGTQSKIHSSNFRFPIGAIAAASGG+SYLYYVS SD+VH
Sbjct: 1   MATFFRRLANAAPSLLRYNALLGTQSKIHSSNFRFPIGAIAAASGGVSYLYYVSSSDMVH 60

Query: 61  LDQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAP 120
           LD +SEEAS KVALKSGKWIEFKLQDSARVTHNT LFRFSFDPSETLGLNVASCIYTRAP
Sbjct: 61  LDPNSEEASSKVALKSGKWIEFKLQDSARVTHNTQLFRFSFDPSETLGLNVASCIYTRAP 120

Query: 121 IGQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIE 180
           IGQDDEGK KYVVRPYTPISDPDA+GYFDLLIKVYPQGKMSQHFA+LKPGDVLEVKGPIE
Sbjct: 121 IGQDDEGKVKYVVRPYTPISDPDARGYFDLLIKVYPQGKMSQHFASLKPGDVLEVKGPIE 180

Query: 181 KLKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD 240
           KLKYSPNMKK IGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD
Sbjct: 181 KLKYSPNMKKRIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD 240

Query: 241 ILASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHIS 300
           ILASSHPN+KVFYTVD P++NWKGGKGYVSKDMVIKGLPPPGDD+LILVCGPPGMMKHIS
Sbjct: 241 ILASSHPNLKVFYTVDNPSQNWKGGKGYVSKDMVIKGLPPPGDDSLILVCGPPGMMKHIS 300

Query: 301 GDKAPDYSQGELDGMLKELGYTKEMVYKF 329
           GDKAPDYSQGELDGMLKELGYTKEMVYKF
Sbjct: 301 GDKAPDYSQGELDGMLKELGYTKEMVYKF 329

[Close]

BLAST of CsaV3_2G002740 vs. ExPASy TrEMBL
Match: A0A1S3C7J4 (NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103497367 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.2e-178
Identity = 311/329 (94.53%), Postives = 320/329 (97.26%), Query Frame = 0


Query: 1   MATFLRRLANAAPS-LRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVH 60
           MATF RRLANAAPS LRYNALLGTQSKIHSSNFRFPIGAIAAASGG+SYLYYVS SD+VH
Sbjct: 1   MATFFRRLANAAPSLLRYNALLGTQSKIHSSNFRFPIGAIAAASGGVSYLYYVSSSDMVH 60

Query: 61  LDQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAP 120
           LD +SEEAS KVALKSGKWIEFKLQDSARVTHNT LFRFSFDPSETLGLNVASCIYTRAP
Sbjct: 61  LDPNSEEASSKVALKSGKWIEFKLQDSARVTHNTQLFRFSFDPSETLGLNVASCIYTRAP 120

Query: 121 IGQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIE 180
           IGQDDEGK KYVVRPYTPISDPDA+GYFDLLIKVYPQGKMSQHFA+LKPGDVLEVKGPIE
Sbjct: 121 IGQDDEGKVKYVVRPYTPISDPDARGYFDLLIKVYPQGKMSQHFASLKPGDVLEVKGPIE 180

Query: 181 KLKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD 240
           KLKYSPNMKK IGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD
Sbjct: 181 KLKYSPNMKKRIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD 240

Query: 241 ILASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHIS 300
           ILASSHPN+KVFYTVD P++NWKGGKGYVSKDMVIKGLPPPGDD+LILVCGPPGMMKHIS
Sbjct: 241 ILASSHPNLKVFYTVDNPSQNWKGGKGYVSKDMVIKGLPPPGDDSLILVCGPPGMMKHIS 300

Query: 301 GDKAPDYSQGELDGMLKELGYTKEMVYKF 329
           GDKAPDYSQGELDGMLKELGYTKEMVYKF
Sbjct: 301 GDKAPDYSQGELDGMLKELGYTKEMVYKF 329

[Close]

BLAST of CsaV3_2G002740 vs. ExPASy TrEMBL
Match: A0A6J1KCV4 (NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111493763 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.5e-161
Identity = 279/328 (85.06%), Postives = 301/328 (91.77%), Query Frame = 0


Query: 1   MATFLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHL 60
           MATFLRRL NAAP+LR NA  G QSKIHSS +RFPIGAIAA SGGISY+YYVS SDLVHL
Sbjct: 1   MATFLRRLVNAAPALRSNA-FGGQSKIHSSKYRFPIGAIAAVSGGISYMYYVSSSDLVHL 60

Query: 61  DQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAPI 120
           DQ SE   PKVALKS KWIEFKLQD+ARVTH+T LFRFSFDP+E LGL+VASC+ TRAP 
Sbjct: 61  DQKSEAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGLHVASCLLTRAPS 120

Query: 121 GQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEK 180
           G D EGK KYVVRPYTPISDPD+KGYFDLLIKVYPQGKMSQHFA+LKPGDV+EVKGPIEK
Sbjct: 121 GVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPQGKMSQHFASLKPGDVVEVKGPIEK 180

Query: 181 LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI 240
           ++YSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSL+YGNVSPDDILLKQKLD+
Sbjct: 181 IRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNVSPDDILLKQKLDL 240

Query: 241 LASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG 300
           LASSHPN+KVFYTVD P K+WKGGKGYV+KDM IKGLP PGDD LILVCGPPGMMKHISG
Sbjct: 241 LASSHPNLKVFYTVDNPPKSWKGGKGYVTKDMAIKGLPAPGDDTLILVCGPPGMMKHISG 300

Query: 301 DKAPDYSQGELDGMLKELGYTKEMVYKF 329
           +KA DYSQGE+ G+L+ELGYTKEMVYKF
Sbjct: 301 EKAKDYSQGEIAGILQELGYTKEMVYKF 327

[Close]

BLAST of CsaV3_2G002740 vs. ExPASy TrEMBL
Match: A0A6J1G357 (NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111450360 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.4e-160
Identity = 279/328 (85.06%), Postives = 301/328 (91.77%), Query Frame = 0


Query: 1   MATFLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHL 60
           MATFLRRL NAAP+L  NA  G QSKIHSS +RFPIGAIAA SGGISY+YYVS SDLVHL
Sbjct: 1   MATFLRRLVNAAPALSSNA-FGGQSKIHSSKYRFPIGAIAAVSGGISYMYYVSSSDLVHL 60

Query: 61  DQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAPI 120
           DQ+SE   PKVALKS KWIEFKLQD+ARVTH+T LFRFSFDP+E LGL+VASC+ TRAP 
Sbjct: 61  DQNSEAGGPKVALKSDKWIEFKLQDTARVTHDTQLFRFSFDPTEKLGLHVASCLLTRAPS 120

Query: 121 GQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEK 180
           G D EGK KYVVRPYTPISDPD+KGYFDLLIKVYP GKMSQHFA+LKPGDV+EVKGPIEK
Sbjct: 121 GVDAEGKVKYVVRPYTPISDPDSKGYFDLLIKVYPHGKMSQHFASLKPGDVVEVKGPIEK 180

Query: 181 LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI 240
           ++YSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSL+YGNVSPDDILLKQKLD+
Sbjct: 181 IRYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLIYGNVSPDDILLKQKLDL 240

Query: 241 LASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG 300
           LASSHPN+KVFYTVD P+K+WKGGKGYVSKDM IKGLP PGDD LILVCGPPGMMKHISG
Sbjct: 241 LASSHPNLKVFYTVDNPSKSWKGGKGYVSKDMAIKGLPAPGDDTLILVCGPPGMMKHISG 300

Query: 301 DKAPDYSQGELDGMLKELGYTKEMVYKF 329
           +KA DYSQGEL G+L+ELGYTKEMVYKF
Sbjct: 301 EKAKDYSQGELAGILQELGYTKEMVYKF 327

[Close]

BLAST of CsaV3_2G002740 vs. ExPASy TrEMBL
Match: A0A6J1CNQ7 (NADH-cytochrome b5 reductase OS=Momordica charantia OX=3673 GN=LOC111013149 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.7e-158
Identity = 272/328 (82.93%), Postives = 298/328 (90.85%), Query Frame = 0


Query: 1   MATFLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVHL 60
           MATF RRLA AAP+L YNA  G QSK  SS FRFPIGAIAA SGGISYLYYVS  DLVHL
Sbjct: 1   MATFFRRLAKAAPALHYNA-CGGQSKFQSSKFRFPIGAIAAVSGGISYLYYVSSPDLVHL 60

Query: 61  DQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAPI 120
           DQS+EE SPKVAL + KWIEFKLQD+ARVTH+T LFRFSFDP++ LGL+ ASC+ TRAP 
Sbjct: 61  DQSNEEGSPKVALNANKWIEFKLQDTARVTHDTQLFRFSFDPTKKLGLDTASCLLTRAPS 120

Query: 121 GQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEK 180
           GQD EGK KYVVRPYTPISD DAKGYFDLLIKVYPQGKMSQHFA+LKPGDV+EVKGPIEK
Sbjct: 121 GQDAEGKIKYVVRPYTPISDSDAKGYFDLLIKVYPQGKMSQHFASLKPGDVVEVKGPIEK 180

Query: 181 LKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDI 240
           ++YSPNMKKHIGMIAGGTGITPMLQVIDAI+KNPDDNTQVSLLYGNVSPDDILLKQKLD+
Sbjct: 181 IRYSPNMKKHIGMIAGGTGITPMLQVIDAILKNPDDNTQVSLLYGNVSPDDILLKQKLDL 240

Query: 241 LASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISG 300
           LA+SHPN+K+FYTVD P+KNWKGG+GY+SKDM +KGLP PG+D  ILVCGPPGMMKHISG
Sbjct: 241 LAASHPNLKIFYTVDNPSKNWKGGQGYISKDMAVKGLPAPGNDTQILVCGPPGMMKHISG 300

Query: 301 DKAPDYSQGELDGMLKELGYTKEMVYKF 329
           +KA DYSQGEL G+LKELGYT+EMVYKF
Sbjct: 301 EKAKDYSQGELSGILKELGYTEEMVYKF 327

[Close]

BLAST of CsaV3_2G002740 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 515.4 bits (1326), Expect = 3.4e-146
Identity = 247/329 (75.08%), Postives = 282/329 (85.71%), Query Frame = 0


Query: 1   MAT-FLRRLANAAPSLRYNALLGTQSKIHSSNFRFPIGAIAAASGGISYLYYVSYSDLVH 60
           MAT F RRLA +AP + +    G+QSK  S  FRF  GAIAA SGG SY Y  S ++LV+
Sbjct: 1   MATSFFRRLARSAP-ITFPVAFGSQSKSGSGAFRFSTGAIAALSGGFSYYYLTSGNNLVY 60

Query: 61  LDQSSEEASPKVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPSETLGLNVASCIYTRAP 120
           LDQ+ EE  PK AL   KW+EFKLQD+ARV+HNT LFRFSFDPS  LGL+VASC+ TRAP
Sbjct: 61  LDQAKEETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSFDPSAELGLHVASCLLTRAP 120

Query: 121 IGQDDEGKAKYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIE 180
           +G + EGK KYV+RPYTPISDP+AKGYFDLLIKVYP GKMSQHFA+LKPGDVLEVKGP+E
Sbjct: 121 LGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPGDVLEVKGPVE 180

Query: 181 KLKYSPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLD 240
           K KYSPNMKKHIGMIAGG+GITPMLQVIDAIVKNP+DNTQ+SLLY NVSPDDILLKQKLD
Sbjct: 181 KFKYSPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPDDILLKQKLD 240

Query: 241 ILASSHPNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHIS 300
           +L ++HPN+K+FYTVD PTKNWKGG GY+SKDM +KGLP P DD LILVCGPPGMM+HIS
Sbjct: 241 VLQANHPNLKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVCGPPGMMEHIS 300

Query: 301 GDKAPDYSQGELDGMLKELGYTKEMVYKF 329
           G KAPD+SQGE+ G+LKELGYT+EMV+KF
Sbjct: 301 GGKAPDWSQGEVKGILKELGYTEEMVFKF 328

[Close]

BLAST of CsaV3_2G002740 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 200.7 bits (509), Expect = 1.9e-51
Identity = 108/261 (41.38%), Postives = 155/261 (59.39%), Query Frame = 0


Query: 70  KVALKSGKWIEFKLQDSARVTHNTHLFRFSFDPS-ETLGLNVASCIYTRAPIGQDDEGKA 129
           +V L    + EFKL    +++HN   F F    S   LGL +   I  R   GQ ++   
Sbjct: 39  RVCLDPENFKEFKLVKRHQLSHNVAKFVFELPTSTSVLGLPIGQHISCRGKDGQGED--- 98

Query: 130 KYVVRPYTPISDPDAKGYFDLLIKVYPQGKMSQHFATLKPGDVLEVKGPIEKLKYSPNMK 189
             V++PYTP +     G F+L+IK+YPQG+MS HF  ++ GD L VKGP  + KY P   
Sbjct: 99  --VIKPYTPTTLDSDVGRFELVIKMYPQGRMSHHFREMRVGDHLAVKGPKGRFKYQPGQF 158

Query: 190 KHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKLDILASSHP-N 249
           +  GM+AGG+GITPM QV  AI++NP D T+V L+Y NV+ DDILLK++L+ L +++P  
Sbjct: 159 RAFGMLAGGSGITPMFQVARAILENPTDKTKVHLIYANVTYDDILLKEELEGLTTNYPEQ 218

Query: 250 IKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGDDALILVCGPPGMMKHISGDKAPDYS 309
            K+FY +++P + W GG G+VSK+M+    P P  D  IL CGPP M K ++ +      
Sbjct: 219 FKIFYVLNQPPEVWDGGVGFVSKEMIQTHCPAPASDIQILRCGPPPMNKAMAAN------ 278

Query: 310 QGELDGMLKELGYTKEMVYKF 329
                  L+ LGY+ EM ++F
Sbjct: 279 -------LEALGYSPEMQFQF 281

[Close]

BLAST of CsaV3_2G002740 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 144.1 bits (362), Expect = 2.1e-34
Identity = 82/240 (34.17%), Postives = 135/240 (56.25%), Query Frame = 0


Query: 79  IEFKLQDSARVTHNTHLFRFSFDPSE-TLGLNVASCIYTRAPIGQDDEGKAKYVVRPYTP 138
           +  +L +   ++H+   FRF+    +  LGL V   I+  A I        K  +R YTP
Sbjct: 663 VPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND------KLCLRAYTP 722

Query: 139 ISDPDAKGYFDLLIKVY-----PQ----GKMSQHFATLKPGDVLEVKGPIEKLKY----- 198
            S  D  GYF+L++K+Y     P+    G MSQ+  +L  G  LE+KGP+  ++Y     
Sbjct: 723 SSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKGS 782

Query: 199 -----SPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQKL 258
                 P     + M+AGGTGITP+ Q+I AI+K+P+D T++ ++Y N + +DILL+++L
Sbjct: 783 FTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLREEL 842

Query: 259 DILASSHPN-IKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGD-DALILVCGPPGMMK 297
           D  A  +P+ +KV+Y V+   + W    G++S+ ++ + +P   D  AL + CGPP M++
Sbjct: 843 DGWAEQYPDRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPPMIQ 896

[Close]

BLAST of CsaV3_2G002740 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 139.8 bits (351), Expect = 3.9e-33
Identity = 81/241 (33.61%), Postives = 138/241 (57.26%), Query Frame = 0


Query: 79  IEFKLQDSARVTHNTHLFRFSFDPSE--TLGLNVASCIYTRAPIGQDDEGKAKYVVRPYT 138
           I  +L +   ++H+   FRF+  PSE   LGL V   ++  A I        K  +R YT
Sbjct: 663 IPVRLIEKTSISHDVRKFRFAL-PSEDQQLGLPVGKHVFVCANIND------KLCLRAYT 722

Query: 139 PISDPDAKGYFDLLIKVY-----PQ----GKMSQHFATLKPGDVLEVKGPIEKLKY---- 198
           P S  DA G+ DL++KVY     P+    G MSQH  +L  G ++++KGP+  ++Y    
Sbjct: 723 PTSAIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKG 782

Query: 199 ------SPNMKKHIGMIAGGTGITPMLQVIDAIVKNPDDNTQVSLLYGNVSPDDILLKQK 258
                  P   K + M+AGGTGITP+ Q+I +I+ +P+D T++ ++Y N + DDIL++++
Sbjct: 783 NFLVSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREE 842

Query: 259 LDILASSH-PNIKVFYTVDKPTKNWKGGKGYVSKDMVIKGLPPPGD-DALILVCGPPGMM 297
           L+  AS H   +K++Y V+   + W    G++++ ++ + +P   + ++L L CGPP M+
Sbjct: 843 LEGWASKHKERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMI 896

The following BLAST results are available for this feature:

BLAST of CsaV3_2G002740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139319.1	8.9e-189	100.00	NADH-cytochrome b5 reductase-like protein [Cucumis sativus] >KAE8651527.1 hypoth...	[more]
XP_008457751.1	2.4e-178	94.53	PREDICTED: NADH-cytochrome b5 reductase-like protein [Cucumis melo] >KAA0061593....	[more]
XP_038889566.1	1.6e-174	93.29	NADH-cytochrome b5 reductase-like protein [Benincasa hispida]	[more]
XP_023547148.1	2.1e-161	85.37	NADH-cytochrome b5 reductase-like protein [Cucurbita pepo subsp. pepo] >XP_02354...	[more]
XP_022999370.1	1.3e-160	85.06	NADH-cytochrome b5 reductase-like protein [Cucurbita maxima] >XP_022999371.1 NAD...	[more]

BLAST of CsaV3_2G002740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83291	4.8e-145	75.08	NADH-cytochrome b5 reductase-like protein OS=Arabidopsis thaliana OX=3702 GN=CBR...	[more]
A1CRK9	1.5e-66	50.79	NADH-cytochrome b5 reductase 2 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 5...	[more]
Q2UKB8	2.6e-66	48.24	NADH-cytochrome b5 reductase 2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40...	[more]
Q2HG02	4.4e-66	41.94	NADH-cytochrome b5 reductase 2 OS=Chaetomium globosum (strain ATCC 6205 / CBS 14...	[more]
Q0CRD8	7.6e-66	49.21	NADH-cytochrome b5 reductase 2 OS=Aspergillus terreus (strain NIH 2624 / FGSC A1...	[more]

BLAST of CsaV3_2G002740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V0C8	1.2e-178	94.53	NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3C7J4	1.2e-178	94.53	NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103497367 PE=3 SV=1	[more]
A0A6J1KCV4	6.5e-161	85.06	NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111493763 PE=3 SV...	[more]
A0A6J1G357	1.4e-160	85.06	NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111450360 PE=3 ...	[more]
A0A6J1CNQ7	6.7e-158	82.93	NADH-cytochrome b5 reductase OS=Momordica charantia OX=3673 GN=LOC111013149 PE=3...	[more]

BLAST of CsaV3_2G002740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20080.1	3.4e-146	75.08	FAD/NAD(P)-binding oxidoreductase	[more]
AT5G17770.1	1.9e-51	41.38	NADH:cytochrome B5 reductase 1	[more]
AT1G37130.1	2.1e-34	34.17	nitrate reductase 2	[more]
AT1G77760.1	3.9e-33	33.61	nitrate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001834	NADH:cytochrome b5 reductase-like	PRINTS	PR00406	CYTB5RDTASE	coord: 106..117 score: 35.24 coord: 285..293 score: 63.43 coord: 230..241 score: 54.51 coord: 169..183 score: 43.06 coord: 192..211 score: 64.69
IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	PRINTS	PR00371	FPNCR	coord: 192..211 score: 40.46 coord: 230..241 score: 30.65 coord: 133..140 score: 44.83 coord: 285..293 score: 44.57
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 193..299 e-value: 2.7E-31 score: 108.5
IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 81..183 e-value: 2.6E-24 score: 85.5
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 176..328 e-value: 3.8E-43 score: 148.7
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 170..327
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 71..175 e-value: 3.3E-35 score: 122.7
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 45..328
None	No IPR available	PANTHER	PTHR19370:SF171	NADH-CYTOCHROME B5 REDUCTASE-LIKE	coord: 45..328
None	No IPR available	CDD	cd06183	cyt_b5_reduct_like	coord: 81..328 e-value: 2.31438E-112 score: 323.75
IPR017927	FAD-binding domain, ferredoxin reductase-type	PROSITE	PS51384	FAD_FR	coord: 76..184 score: 13.96779
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 69..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_2G002740.1	CsaV3_2G002740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity