CsaV3_2G002470 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_2G002470
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	tRNA (guanine(37)-N1)-methyltransferase
Location	chr2: 1027298 .. 1031286 (+)
RNA-Seq Expression	CsaV3_2G002470
Synteny	CsaV3_2G002470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACGCTATGTTTTGTGTTATATTGTTTCGATTTCAATTAATCATTTGGGTAATGGCATTTAGCATTAGTGTTACTCATATATTGTTGTCGTTGAACTGTTCATCTTTCTTATCCGAGTTCATGTTTTTGAAAACTTATCAGAATTCTCCTGATATTTTTTAGTTTTTTTCTTTGAAAAGTATTCTTGTTATTAACTTTTGTTTAAAAGCAGACTTTTCTTTGGCCCTCAGTTCGTTTCTGTTGAAAAATTTTCAGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGGTAAAGATCTCCATCTCCGTCCTCATATTTTTTCTGTTAACTATAAGCTTGGATTGCAATTTCATGTTCTTTATGGAAACTCTTCTGTAGCACATTCTGTTCGCTGAAGTTCCAATTCTTGTTCATTGATTTTCTTCATGTTACCTTAGTGGCTTTCTGGCTCTGTTCATGCATTCAAGTTCTTAAATAGTTCTAAAATTATCGAGCGAGTAATTTTTTATCTTAAAACTTCAATTTCTGTAGGGTATAACCATCAACTTGAATGCATCTTTAGTTAGAAAAGTGTCTAAAAACAGTCTATATATCACATGAAACCAAATTAACGTCGTGTAACTAGGTAATTTTCATAAAAATAATCTCGTTTCCCTTTAAAAATCACTCATTTTTTCTCTTCCTTCTTTTTACCTTATTTTCTCAATGGAATTTTCCTCGATCATGTCCTTAGATGACACCATTTTTCTATCTTGTTGATTCTTTGAATGCTCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTATTTTCAATAATTCTTCCCTTTATCACACATTGTGCAAATTTTACTTTCACCTTAGGATTGCCTGTTGAACTCCAGATATTTATAGATGCCTTTAACTTTCGAGTTGCAAGTCTGAAGTGTTATGTAAGGCATATCTAGCATCATTTTTAATGATCCCATTCCCATGACTCAAAATCCAAACGTTTTTTACATTGTGACTGCACATGTGCTGATAGAATGTGGTTGTCTTTGCCTCTCATTTGATATTATAACTACATGTGATGTGACCGTTCTACATCAAGCGGTTGCAATTATAAGAACAATGTTCGATTTCTGAAAGATAGTTTAAACAATTCTGTGAGCGAATTTACCCGTCATTTGATGTGTTTGTATTGTACATACGTAACTAACTTACTGTGTGGTTGCTCTGTAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCATGTTCCACCTACGCTTTCCTTTCTACATTTTGTATCCATTTCCATATGCTCTTTATAGCTTTTATGTGCGTTCTTTTGTTGCTCTGTATTACATTTTGGTACCACTTGTTTATGTGACATTGGTTGTAACGTTGCTTTTTCATGTACAACTATTAACATTGAGGTAATTTTGTACTGTGATTTGCAGGTCACGTTGCTCACCTGAATATACATGACGAATTACTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATGTATGTCATCAGTATCTATTTACTTATTTTCCTAATTATTTTGAAATGTACATTGTGATTTATTAATCACTATAATTTAGCTGTTAGTTCATTTTTTTATTCTTAATTTTGTTTTACTCTCCCCTTCTTTTCCTCCGTGCATATGTTGGGGGTACCTTTTTAAGAAATTCAAATGTTCAGAATCGGATATTTAATATTTTTATCCTCCCCCTTCATCCAGCAATATGCACACACACACATTTTCCATATTGAAACTTTTTCTGTAACTATTTCTTTTTGTAATCTTTGCAGAAAAATTATCCCAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCGACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCATCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGAAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATTGGCAAATGGTATGCCGACTGGTATTATATTATTATTATCATTTAAAGAAAATTTCACATCGTCTTCAGGAGATTAGTGTAGATGATCTTAATTTTCTTTCTTGCTTCAGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACAATCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGGTAACATACTCTACCATAGTTTGTGATTGAACAACCAAGACCGTTTCTTCCTATAATTTTTTTTGCAGATTTTTTTTAATGAAAATTTGCTTTGCAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGGTACGAATGACCTTTTTTCTTTTTAAATTTCATTTCACTTATTGCAGTTGTTTTAGCATATTTCTCTACATTTGTGTGTCAGAATGAAGAGTTAAAAGTTGGGGAAATAACAATGTTAGGAAAGGGAAACAATTACTTTTTAATGCTTCAAATATGAAGGGAAACCATAAACGATTTTTTCTCTCTTACTATCACCATGACAGATATTTTGGATATGTCATATTTCCTAATTCCTACCTTTCAAATGGAATTCTTTGTTGTAAGTTTTGTAGTCACTCTCTATACCAATTTTATGAGTTTCTTCAATCTCCTTTAGCTCTTGGGTGAGGGAGAATGCCTTATCTCCTCCACTCAATTATCTCAACTCGTGTTTTTTATCCATATCTATATTTTGCTTAGTTAACCAATGAAGTATATTTTGTTATTTTCTTGCCAGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGTATGTGTATACTAAATTACTATAGATCCTTATTTGTCTGTCTTCTTTTTGATAATTGTATTTCTTTCTCACCATTCTATTTGGATATCTGTCTTGAAAAAAGTTGTCTTTCTTCTCATACCATCCTCTTTTAACTGTTGCACTCCAGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGTATTGCTTTGTGTGTGTGTGTATAAATGGAAACTACTTGTTTTTAACTCTTTTTTCTTTTTTGTTTTATCAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAA

mRNA sequence

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCATGTTCCACCTACGCTTTCCTTTCTACATTTTAAAAATTATCCCAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCGACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCATCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGAAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATTGGCAAATGGTATGCCGACTGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACAATCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAA

Coding sequence (CDS)

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCATGTTCCACCTACGCTTTCCTTTCTACATTTTAAAAATTATCCCAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCGACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCATCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGAAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATTGGCAAATGGTATGCCGACTGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACAATCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAA

Protein sequence

MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKVQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIVKYSNPCHVPPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVDEDTTS*

Homology

[Close]

BLAST of CsaV3_2G002470 vs. NCBI nr
Match: KAE8651523.1 (hypothetical protein Csa_019510 [Cucumis sativus])

HSP 1 Score: 995.7 bits (2573), Expect = 1.4e-286
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYG 180
           VKYSNPCHVPPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYG
Sbjct: 121 VKYSNPCHVPPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYG 180

Query: 181 ATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLN 240
           ATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLN
Sbjct: 181 ATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLN 240

Query: 241 PDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGG 300
           PDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGG
Sbjct: 241 PDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGG 300

Query: 301 TNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIAD 360
           TNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIAD
Sbjct: 301 TNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIAD 360

Query: 361 DASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWK 420
           DASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWK
Sbjct: 361 DASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWK 420

Query: 421 GSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQ 480
           GSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQ
Sbjct: 421 GSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQ 480

Query: 481 CVDEDTTS 489
           CVDEDTTS
Sbjct: 481 CVDEDTTS 488

[Close]

BLAST of CsaV3_2G002470 vs. NCBI nr
Match: XP_031736378.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 944.1 bits (2439), Expect = 4.7e-271
Identity = 471/495 (95.15%), Postives = 476/495 (96.16%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L +       ++ KNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA
Sbjct: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
           CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE
Sbjct: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420
           KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS
Sbjct: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420

Query: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480
           IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL
Sbjct: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480

Query: 481 SFRLPEQCVDEDTTS 489
           SFRLPEQCVDEDTTS
Sbjct: 481 SFRLPEQCVDEDTTS 495

[Close]

BLAST of CsaV3_2G002470 vs. NCBI nr
Match: XP_011648665.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 929.5 bits (2401), Expect = 1.2e-266
Identity = 467/495 (94.34%), Postives = 472/495 (95.35%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L +       ++ KNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA
Sbjct: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
           CEKGIGGTNQESPLAN    VQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE
Sbjct: 301 CEKGIGGTNQESPLAN----VQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420
           KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS
Sbjct: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420

Query: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480
           IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL
Sbjct: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480

Query: 481 SFRLPEQCVDEDTTS 489
           SFRLPEQCVDEDTTS
Sbjct: 481 SFRLPEQCVDEDTTS 491

[Close]

BLAST of CsaV3_2G002470 vs. NCBI nr
Match: XP_008458074.1 (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo])

HSP 1 Score: 891.7 bits (2303), Expect = 2.8e-255
Identity = 452/496 (91.13%), Postives = 466/496 (93.95%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L +       ++ KNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSE+VTSILKA
Sbjct: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSILKA 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
            E+GIGGTNQESPLAN    VQVQEVQD I+ISNK VE SRSADISVAVLKRSSESCENE
Sbjct: 301 SEEGIGGTNQESPLAN----VQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENE 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRG 420
           KD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRG
Sbjct: 361 KDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRG 420

Query: 421 SIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYC 480
           SIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYC
Sbjct: 421 SIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYC 480

Query: 481 LSFRLPEQCVDEDTTS 489
           LSFRLPEQCV+EDTTS
Sbjct: 481 LSFRLPEQCVNEDTTS 491

[Close]

BLAST of CsaV3_2G002470 vs. NCBI nr
Match: KAA0061573.1 (tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 890.6 bits (2300), Expect = 6.2e-255
Identity = 455/505 (90.10%), Postives = 465/505 (92.08%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPC---HV-------------PPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFE 180
                 C   HV                   ++ KNYPRIKTVVNKVGSITNEFRVPKFE
Sbjct: 121 GDNFVLCFAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180

Query: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240
           ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA
Sbjct: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240

Query: 241 IPAAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300
           IPAAQKECIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS
Sbjct: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300

Query: 301 ESVTSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLK 360
           E+VTSILKA E+GIGGTNQESPLAN    VQVQEVQD I+ISNK VE SRSADISVAVLK
Sbjct: 301 ETVTSILKASEEGIGGTNQESPLAN----VQVQEVQD-IEISNKFVEHSRSADISVAVLK 360

Query: 361 RSSESCENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASA 420
           RSSESCENEKD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASA
Sbjct: 361 RSSESCENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASA 420

Query: 421 LHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRD 480
           LHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRD
Sbjct: 421 LHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRD 480

Query: 481 VAPNKAMYCLSFRLPEQCVDEDTTS 489
           VAPNKAMYCLSFRLPEQCV+EDTTS
Sbjct: 481 VAPNKAMYCLSFRLPEQCVNEDTTS 500

[Close]

BLAST of CsaV3_2G002470 vs. ExPASy Swiss-Prot
Match: F6HH45 (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0016g04350 PE=3 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 2.1e-181
Identity = 324/503 (64.41%), Postives = 386/503 (76.74%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           +LDESKFD+ L LWALRIP +LCK A +ILNGYL+DKPR+KP+TEDPTC+ NRY+ILSE+
Sbjct: 2   LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           ++ P+LS+IPE K +ELK LC  E VPYSLTLGYSYW ADH+LK+ILP  VEVPSSFETI
Sbjct: 62  IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121

Query: 121 VKYSNPCHV-------------PPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILK 180
               + CH+                   ++ KNYPRIKTVVNKVG+ITNEFRVPKFEIL 
Sbjct: 122 T--FSLCHIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILV 181

Query: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240
           G++DMVTEVKQY ATFKLDYSLVYWNSRLEHEH+RLVS F+PG++ICDMF+G+GPFAIPA
Sbjct: 182 GKDDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIPA 241

Query: 241 AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300
           AQK C+VYANDLNPDSIRYLK+NA+INKV   +  YN+DARKFISQLM VP +E   E  
Sbjct: 242 AQKGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEPD 301

Query: 301 TSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRS-ADISVAVLKRS 360
             +LKA E+     N ++   NG  TV+   V      +   +E S + AD +VA +K+ 
Sbjct: 302 VPVLKATEECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKKH 361

Query: 361 SESCENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALH 420
           S SCE E  NG  D+A+  V GRR    NKRM+GS S S+ + WEHVDHVIMNLPASA+ 
Sbjct: 362 SGSCEEE--NGTYDNATVSVVGRRKDRMNKRMRGSESSSNIKPWEHVDHVIMNLPASAIQ 421

Query: 421 FLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVA 480
           FLDAFRG I++KYWKGSLPWIHCYCF+RANE  + IISEAE+ALNA+IQ+PI HRVRDVA
Sbjct: 422 FLDAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMIISEAETALNASIQEPILHRVRDVA 481

Query: 481 PNKAMYCLSFRLPEQ-CVDEDTT 488
           PNKAM+CLSFRL E+ C  ED T
Sbjct: 482 PNKAMFCLSFRLSEEACFKEDAT 500

[Close]

BLAST of CsaV3_2G002470 vs. ExPASy Swiss-Prot
Match: Q93YU6 (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 1.7e-159
Identity = 288/494 (58.30%), Postives = 357/494 (72.27%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW ADH+LK+ILP  +++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L F       ++ KNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN M 
Sbjct: 121 GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENGME 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK C 
Sbjct: 181 TEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKGCF 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDS+RYLK+NA+ NKV   + V+N+DARKF S LM V   E N +SV    K 
Sbjct: 241 VYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADNDKT 300

Query: 301 CEKGI--GGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCE 360
            E  +  GG    S               + I+ SN S+ +   A+      K+ S + +
Sbjct: 301 KEAAVSRGGETNSS--------------GEEIRESNASINEPLGAN------KKPSGTTK 360

Query: 361 NEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFR 420
            E  NG+  D  +       G+ NKR++ + +  A+ WEH+DHVIMNLPASAL FLD+F 
Sbjct: 361 TE--NGVGKDCKS-----IEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFS 420

Query: 421 GSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMY 480
             I+KKYWKG LP IHCYCF+RA+E  +FII+EAE+AL  +I+DP+FH+VRDVAPNKAM+
Sbjct: 421 NVIQKKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMF 467

Query: 481 CLSFRLPEQCVDED 486
           CLSFRLPE C+ ++
Sbjct: 481 CLSFRLPEACLKQE 467

[Close]

BLAST of CsaV3_2G002470 vs. ExPASy Swiss-Prot
Match: C5XX79 (tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g025390 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 1.9e-145
Identity = 268/485 (55.26%), Postives = 341/485 (70.31%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKIL-NGYLIDKPRIKPVTEDPTCDKNRYVILSEK 61
           LDESKF+  L LWALRIPREL  A  ++L +GYL+DKPR+K V EDP  DKNR VILSEK
Sbjct: 4   LDESKFEQRLQLWALRIPRELSSAVTRLLRSGYLLDKPRVKTVVEDPEGDKNRLVILSEK 63

Query: 62  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 121
           +Q P+LS++P+ +L+ LK LC  + VPY+LTLGYSYW A H+    +          + +
Sbjct: 64  IQKPDLSDMPQQELDSLKQLCNVDVVPYTLTLGYSYWSAGHVAHLNIS---------DDL 123

Query: 122 VKYSNPCHVPPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYG 181
           + Y N          ++ KNYPRI+TV NKVG+ITNEFRVPKFEIL G+NDMVTEVKQYG
Sbjct: 124 LPYKN-----VIAKVIYDKNYPRIQTVANKVGTITNEFRVPKFEILAGKNDMVTEVKQYG 183

Query: 182 ATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLN 241
           ATFKLDY LVYWNSRL+HEHIRLVSLF+ G+VICDMFAGIGPFAIPAAQK C+VYANDLN
Sbjct: 184 ATFKLDYGLVYWNSRLDHEHIRLVSLFKKGDVICDMFAGIGPFAIPAAQKGCVVYANDLN 243

Query: 242 PDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGG 301
           PDS+ YL+ NA+INKV   +  YN+DAR F+  LM VP  E+ S+   +  ++  K  G 
Sbjct: 244 PDSVHYLRTNAKINKVDDYIFAYNMDARVFMQNLMTVPGLETGSDCQVAADESYPKE-GV 303

Query: 302 TNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIAD 361
              E+  +NG          D  + S     +S  A  +    +++SE CE++  +G  D
Sbjct: 304 PANENSSSNG-------NHNDVREGSQNGANESSVASTTAKKRQQTSEECESDCQDG--D 363

Query: 362 DASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWK 421
            + T+   RRN   NKR++G     ++ WEH DHV+MNLPASAL FLD F G ++KKYW 
Sbjct: 364 ASQTK---RRN---NKRVRGPGPPPSKPWEHFDHVLMNLPASALQFLDCFDGLVQKKYWT 423

Query: 422 GSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQ 481
           GSLPWIHCYCF+R++E+++ I+S   + LNA I +PIFHRVRDVAPNKAM+CLSF+LP +
Sbjct: 424 GSLPWIHCYCFIRSSESEESILS---NKLNAKIAEPIFHRVRDVAPNKAMFCLSFKLPME 455

Query: 482 CVDED 486
           C+ ED
Sbjct: 484 CLRED 455

[Close]

BLAST of CsaV3_2G002470 vs. ExPASy Swiss-Prot
Match: A9T6G5 (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 6.1e-104
Identity = 204/477 (42.77%), Postives = 280/477 (58.70%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           +D+S F V L   ALRIP++       +L+ YL+D PR+K + EDP     R ++ SE +
Sbjct: 11  IDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAI 70

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIV 121
             P+L  + E K+  +K +   + V + + L YSYW  +HILK+ILP   EVPSSFETI 
Sbjct: 71  AQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETI- 130

Query: 122 KYSNPCHVPPTLSFLHFKNY---------PRIKTVVNKVGSITNEFRVPKFEILKGENDM 181
              +  H+      L +K           P++KTVVNKVG+ITNEFRVP+FEIL GE  +
Sbjct: 131 --GHIAHLNLREDLLTYKKIIAEVILDKNPKLKTVVNKVGTITNEFRVPEFEILAGEPSL 190

Query: 182 VTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKEC 241
           VTE+KQ+GATF+LDY +VYWNSRLE EH RL + F+PG+VI DMFAGIGPFAIPAAQ+ C
Sbjct: 191 VTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPAAQQGC 250

Query: 242 IVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILK 301
            VYANDLNP S+++LK+N++INKV   +  +NLDAR+F+ +L  V   E     + S   
Sbjct: 251 AVYANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKL--VTEEEGCVGEIASSTL 310

Query: 302 ACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCEN 361
             E  + GT   S   N     +  ++    K   +  ++++ +++              
Sbjct: 311 IAENSLNGTRIPSSKENSSSLTEGSDMPSSKKPEERKAKKAKVSEV-------------- 370

Query: 362 EKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRG 421
                                   +MK   +S  + WEH DHV+MNLPASAL FLD   G
Sbjct: 371 -----------------------VKMK---ISDVKPWEHFDHVVMNLPASALEFLDVLNG 430

Query: 422 SIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKA 470
            + K  WKG++P +HCYCFMR+NE    I+ +AE+ L  +I DP  + VRDVAPNKA
Sbjct: 431 LLCKGRWKGTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKA 442

[Close]

BLAST of CsaV3_2G002470 vs. ExPASy Swiss-Prot
Match: F4NUJ6 (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=TRM5 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.3e-66
Identity = 158/492 (32.11%), Postives = 249/492 (50.61%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           L+ + F+    L AL+IP      A++ L  +++  PR++ + +DPT  K R ++L   +
Sbjct: 16  LNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLDPSM 75

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIV 121
           +  E++++P   L+E       + V +++ L Y YW +D +L+ ILP E+E P +FET+ 
Sbjct: 76  EASEINDLPN-PLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSILPDEMETPGAFETV- 135

Query: 122 KYSNPCHVPPTLSFLHFKNY---------PRIKTVVNKVGSITNEFRVPKFEILKGENDM 181
              +  H+     +  FK+            IKTVVNK+ +I + FR  + EIL G NDM
Sbjct: 136 --GHIAHLNLRDRYQPFKHIIGQVILDKSSHIKTVVNKLDNIDHTFRFFQMEILAGINDM 195

Query: 182 VTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQ-KE 241
             ++K+ G  F  D+S VYWNSRL+ EH R++ LF   ++ICD+FAG+GPFA+PAA+ K 
Sbjct: 196 NAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPFALPAAKHKR 255

Query: 242 CIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 301
           C+V+ANDLNP S +YL  N ++NK+  R+  +N+D R+FI Q +     + N+ ++ + +
Sbjct: 256 CVVFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQSL----EDLNNPAIWNKI 315

Query: 302 KACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCE 361
                      ++ P +N              K  N+ VE    A ++           +
Sbjct: 316 ----------TKQKPTSND-------------KKRNRKVESPTVAPLT-----------D 375

Query: 362 NEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFR 421
               +GI                                H  H +MNLPA+A+ FLDAF 
Sbjct: 376 QPAISGI-------------------------------RHFKHYVMNLPATAIEFLDAFH 434

Query: 422 G---SIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIF--HRVRDVAP 479
           G    ++       LP IHC+CF  A + +  +I   E  +   +   +   H VR VAP
Sbjct: 436 GLYSGMRDVIMDSDLPTIHCHCFSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVAP 434

[Close]

BLAST of CsaV3_2G002470 vs. ExPASy TrEMBL
Match: A0A0A0LFW8 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G005350 PE=3 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 1.5e-267
Identity = 469/501 (93.61%), Postives = 472/501 (94.21%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHV-------------PPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILK 180
           +     CHV                   ++ KNYPRIKTVVNKVGSITNEFRVPKFEILK
Sbjct: 121 LL----CHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILK 180

Query: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240
           GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA
Sbjct: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240

Query: 241 AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300
           AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV
Sbjct: 241 AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300

Query: 301 TSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSS 360
           TSILKACEKGIGGTNQESPLAN    VQVQEVQDYIKISNKSVEQSRSADISVAVLKRSS
Sbjct: 301 TSILKACEKGIGGTNQESPLAN----VQVQEVQDYIKISNKSVEQSRSADISVAVLKRSS 360

Query: 361 ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL 420
           ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL
Sbjct: 361 ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL 420

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480
           DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN
Sbjct: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480

Query: 481 KAMYCLSFRLPEQCVDEDTTS 489
           KAMYCLSFRLPEQCVDEDTTS
Sbjct: 481 KAMYCLSFRLPEQCVDEDTTS 493

[Close]

BLAST of CsaV3_2G002470 vs. ExPASy TrEMBL
Match: A0A1S3C7L3 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.4e-255
Identity = 452/496 (91.13%), Postives = 466/496 (93.95%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L +       ++ KNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSE+VTSILKA
Sbjct: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSILKA 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
            E+GIGGTNQESPLAN    VQVQEVQD I+ISNK VE SRSADISVAVLKRSSESCENE
Sbjct: 301 SEEGIGGTNQESPLAN----VQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENE 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRG 420
           KD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRG
Sbjct: 361 KDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRG 420

Query: 421 SIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYC 480
           SIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYC
Sbjct: 421 SIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYC 480

Query: 481 LSFRLPEQCVDEDTTS 489
           LSFRLPEQCV+EDTTS
Sbjct: 481 LSFRLPEQCVNEDTTS 491

[Close]

BLAST of CsaV3_2G002470 vs. ExPASy TrEMBL
Match: A0A5A7V0B1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001350 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 3.0e-255
Identity = 455/505 (90.10%), Postives = 465/505 (92.08%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPC---HV-------------PPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFE 180
                 C   HV                   ++ KNYPRIKTVVNKVGSITNEFRVPKFE
Sbjct: 121 GDNFVLCFAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180

Query: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240
           ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA
Sbjct: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240

Query: 241 IPAAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300
           IPAAQKECIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS
Sbjct: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300

Query: 301 ESVTSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLK 360
           E+VTSILKA E+GIGGTNQESPLAN    VQVQEVQD I+ISNK VE SRSADISVAVLK
Sbjct: 301 ETVTSILKASEEGIGGTNQESPLAN----VQVQEVQD-IEISNKFVEHSRSADISVAVLK 360

Query: 361 RSSESCENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASA 420
           RSSESCENEKD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASA
Sbjct: 361 RSSESCENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASA 420

Query: 421 LHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRD 480
           LHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRD
Sbjct: 421 LHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRD 480

Query: 481 VAPNKAMYCLSFRLPEQCVDEDTTS 489
           VAPNKAMYCLSFRLPEQCV+EDTTS
Sbjct: 481 VAPNKAMYCLSFRLPEQCVNEDTTS 500

[Close]

BLAST of CsaV3_2G002470 vs. ExPASy TrEMBL
Match: A0A5D3CFY1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00100 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.3e-244
Identity = 435/489 (88.96%), Postives = 451/489 (92.23%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA H+    +  E+ +P   + I
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDEL-LPYK-DVI 120

Query: 121 VKYSNPCHVPPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYG 180
            K             ++ KNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYG
Sbjct: 121 AK------------VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYG 180

Query: 181 ATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLN 240
           ATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLN
Sbjct: 181 ATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLN 240

Query: 241 PDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGG 300
           PDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSE+VTSILKA E+GIGG
Sbjct: 241 PDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGG 300

Query: 301 TNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNGIAD 360
           TNQESPLAN    VQVQEVQD I+ISNK VE SRSADISVAVLKRSSESCENEK++ IAD
Sbjct: 301 TNQESPLAN----VQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIAD 360

Query: 361 DASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYW 420
           DASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRGSIKKKYW
Sbjct: 361 DASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYW 420

Query: 421 KGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPE 480
           KGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPE
Sbjct: 421 KGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPE 470

Query: 481 QCVDEDTTS 489
           QCV+EDTTS
Sbjct: 481 QCVNEDTTS 470

[Close]

BLAST of CsaV3_2G002470 vs. ExPASy TrEMBL
Match: A0A6J1DUW2 (tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024249 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.6e-232
Identity = 405/491 (82.48%), Postives = 438/491 (89.21%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCKAAIKILNGYLID+ R+KPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKAAIKILNGYLIDRARVKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ PELS+IPE KLEELKGLC  E VPYSLTLGYSYWGADHILK+ILPP VEVPSSFETI
Sbjct: 61  VQAPELSQIPEAKLEELKGLCEIELVPYSLTLGYSYWGADHILKQILPPRVEVPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L +       ++ KNYPRIKTVVNKVG+ITN+FRVPKFEIL GE+DMV
Sbjct: 121 GHIAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNKFRVPKFEILMGEHDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDY LVYWNSRLEHEH+RLVSLFQPGE+ICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYGLVYWNSRLEHEHVRLVSLFQPGEIICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VY NDLNPDS++YLKVNAEINKVS RVHVYNLDARKFISQLMIVP NESNSESVTS+LKA
Sbjct: 241 VYGNDLNPDSVQYLKVNAEINKVSDRVHVYNLDARKFISQLMIVPDNESNSESVTSVLKA 300

Query: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360
           C+KGIGG+NQESP AN    VQV+EVQ+  KIS+++VE SRSADISV+VLKRSSESC  E
Sbjct: 301 CDKGIGGSNQESPSAN----VQVEEVQENSKISDETVEHSRSADISVSVLKRSSESC--E 360

Query: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGS 420
           KD+GI DDAS  VAGR+  N NKRMKGS++ HARTWEHVDHVIMNLPASAL FLDAFRGS
Sbjct: 361 KDDGIVDDASISVAGRKKRNANKRMKGSNICHARTWEHVDHVIMNLPASALQFLDAFRGS 420

Query: 421 IKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCL 480
           I++KYWKGSLPWIHCYCFMRANENQDFIISEAESALN  IQDP+FHRVRDVAPNKAMYCL
Sbjct: 421 IRRKYWKGSLPWIHCYCFMRANENQDFIISEAESALNGKIQDPVFHRVRDVAPNKAMYCL 480

Query: 481 SFRLPEQCVDE 485
           SFRLPEQC +E
Sbjct: 481 SFRLPEQCFNE 485

[Close]

BLAST of CsaV3_2G002470 vs. TAIR 10
Match: AT3G56120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 563.9 bits (1452), Expect = 1.2e-160
Identity = 288/494 (58.30%), Postives = 357/494 (72.27%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW ADH+LK+ILP  +++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 VKYSNPCHVPPTLSF-------LHFKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
              ++       L F       ++ KNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN M 
Sbjct: 121 GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENGME 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK C 
Sbjct: 181 TEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKGCF 240

Query: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300
           VYANDLNPDS+RYLK+NA+ NKV   + V+N+DARKF S LM V   E N +SV    K 
Sbjct: 241 VYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADNDKT 300

Query: 301 CEKGI--GGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCE 360
            E  +  GG    S               + I+ SN S+ +   A+      K+ S + +
Sbjct: 301 KEAAVSRGGETNSS--------------GEEIRESNASINEPLGAN------KKPSGTTK 360

Query: 361 NEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFR 420
            E  NG+  D  +       G+ NKR++ + +  A+ WEH+DHVIMNLPASAL FLD+F 
Sbjct: 361 TE--NGVGKDCKS-----IEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFS 420

Query: 421 GSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMY 480
             I+KKYWKG LP IHCYCF+RA+E  +FII+EAE+AL  +I+DP+FH+VRDVAPNKAM+
Sbjct: 421 NVIQKKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMF 467

Query: 481 CLSFRLPEQCVDED 486
           CLSFRLPE C+ ++
Sbjct: 481 CLSFRLPEACLKQE 467

[Close]

BLAST of CsaV3_2G002470 vs. TAIR 10
Match: AT4G27340.1 (Met-10+ like family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.8e-42
Identity = 126/442 (28.51%), Postives = 187/442 (42.31%), Query Frame = 0


Query: 53  RYVILSEKVQTPELSEIPE------MKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 112
           R ++L EK    E+ ++PE       + +       FE V   LTL Y YW    IL+ +
Sbjct: 274 RLLLLDEKYSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAV 333

Query: 113 LPPEVEVPSSFETI--VKYSN--PCHVP---PTLSFLHFKNYPRIKTVVNKVGSITNEFR 172
           LP  + VPS+FE +  + + N    H+P        +  KN P+I+TVVNK+  I N+FR
Sbjct: 334 LPKGMIVPSAFEMVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKIDPIHNDFR 393

Query: 173 VPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAG 232
             + E+L G + +VT V + G  F +D + VYWNS+L  E  RL+  F   +V+CD+FAG
Sbjct: 394 TMQLEVLAGNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFDQNDVVCDVFAG 453

Query: 233 IGPFAIPAAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPP 292
           +GP A+ AA+    VYANDLNP ++ +++ N+ +NK+  R+ ++N+D R+FI  +     
Sbjct: 454 VGPIALAAARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIEIFNMDGRRFIKAMF---- 513

Query: 293 NESNSESVTSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADIS 352
                        + EKG                                          
Sbjct: 514 -------------SSEKG------------------------------------------ 573

Query: 353 VAVLKRSSESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNL 412
                                                             + V  V+MNL
Sbjct: 574 --------------------------------------------------QKVTQVVMNL 606

Query: 413 PASALHFLDAFRGSIKKKYWKG--SLPWIHCYCFMRANENQDFIISEAESALNANIQDPI 472
           P  A   LDAFRG    ++     S P IH Y F +A++ +         AL+    D  
Sbjct: 634 PKDAAESLDAFRGVYNDRHRDEGLSFPTIHVYGFSKASDPEFDFHERIRIALSEVAVDVK 606

Query: 473 FHRVRDVAPNKAMYCLSFRLPE 480
             +VR VAP K M C SF LP+
Sbjct: 694 MRKVRLVAPGKWMLCASFILPK 606

[Close]

BLAST of CsaV3_2G002470 vs. TAIR 10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein )

HSP 1 Score: 57.0 bits (136), Expect = 4.9e-08
Identity = 38/115 (33.04%), Postives = 57/115 (49.57%), Query Frame = 0


Query: 156 NEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICD 215
           N  R    EIL G+N  V   ++ G  +  D +   ++     E +R+ ++    EV+ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 216 MFAGIGPFAIP--AAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDAR 269
           +FAGIG F +P     K  +VYA + NP +I  L+ N E N VS R  +   D R
Sbjct: 845 LFAGIGYFVLPFLVRAKAKLVYACEWNPHAIEALRRNVEANSVSERCIILEGDNR 898

The following BLAST results are available for this feature:

BLAST of CsaV3_2G002470 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8651523.1	1.4e-286	100.00	hypothetical protein Csa_019510 [Cucumis sativus]	[more]
XP_031736378.1	4.7e-271	95.15	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus]	[more]
XP_011648665.1	1.2e-266	94.34	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus]	[more]
XP_008458074.1	2.8e-255	91.13	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo]	[more]
KAA0061573.1	6.2e-255	90.10	tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa]	[more]

BLAST of CsaV3_2G002470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F6HH45	2.1e-181	64.41	tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0...	[more]
Q93YU6	1.7e-159	58.30	tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3...	[more]
C5XX79	1.9e-145	55.26	tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g02539...	[more]
A9T6G5	6.1e-104	42.77	tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPA...	[more]
F4NUJ6	2.3e-66	32.11	tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai...	[more]

BLAST of CsaV3_2G002470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFW8	1.5e-267	93.61	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G0053...	[more]
A0A1S3C7L3	1.4e-255	91.13	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 ...	[more]
A0A5A7V0B1	3.0e-255	90.10	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A5D3CFY1	5.3e-244	88.96	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1DUW2	1.6e-232	82.48	tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of CsaV3_2G002470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56120.1	1.2e-160	58.30	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G27340.1	1.8e-42	28.51	Met-10+ like family protein	[more]
AT4G04670.1	4.9e-08	33.04	Met-10+ like family protein / kelch repeat-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.300.110		coord: 136..170 e-value: 3.2E-7 score: 32.3
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 173..477 e-value: 5.8E-58 score: 197.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 351..382
None	No IPR available	PANTHER	PTHR23245	TRNA METHYLTRANSFERASE	coord: 2..482
None	No IPR available	PANTHER	PTHR23245:SF36	TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE	coord: 2..482
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 213..270 e-value: 1.7677E-4 score: 38.9503
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PFAM	PF02475	Met_10	coord: 143..429 e-value: 1.8E-54 score: 184.5
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PROSITE	PS51684	SAM_MT_TRM5_TYW2	coord: 117..479 score: 88.611839
IPR025792	tRNA (guanine(37)-N(1))-methyltransferase, eukaryotic	HAMAP	MF_03152	TRM5	coord: 2..479 score: 22.209696
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 138..479

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_2G002470.1	CsaV3_2G002470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
molecular_function	GO:0009019	tRNA (guanine-N1-)-methyltransferase activity