CsaV3_1G043860 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_1G043860
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionRING-type E3 ubiquitin transferase
Locationchr1: 29011462 .. 29014704 (-)
RNA-Seq ExpressionCsaV3_1G043860
SyntenyCsaV3_1G043860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGGGCTGATAACTTTTCCCATCTCTTTCCTCCGTCATTCACCTCCATATATAAAAGCCACAAAGTCCTCCAAAAGAAAGAAGCAATGTTTACACATTACTCTCATTTATCTCTGTGGCTCCATCTTCATGTTCTCAAAGCATCAACCTTCACTAAGGTGTGGTACTATCCATGGCAGAAATCGAGGTTCCTTCTGACTTTTTGTGCCCCATTTCCCTACAACTCATGAGAGATCCGGTAACGATCTCAACCGGGATCACATACGACCGAGAGAGTATCAAGAAATGGCTCTCATCTTGCAAAAACCAACCCCTGAGCTGTCCGGTGTCCAAGCAGGCTCTAAGCAGTGTGGATTTGACACCGAACCATACGCTGCGACGGGTGATCCAAGGGTGGTGTAGTTTGAATGAAAGACATGGGGTGGAGCAGATCCCAACCCCTGAGGATGAGAGTGATAGAGGAGATGTTGTTAAGTTGATCCTTAAGGAGGCAATGAAAAGTCCAAGAAGCTCAATGCTTGAGTGCTTGAAGAGATTGAAATCAATTGTGGCTGAGAATGAGAGCAACAAAATCTACTTGCAGGCAAGCATTAAATATTCCATTTAGTGCTCTTTTAAAATCGATTTGATTTTCTTTTCAAGATCTTCATAATCTGTTTTTGGACTGTTATATAAATAACGTGTTACAAATAATGAACTTAAGGTATATTACACACTCATTTGTTTAATGGTTATTTTAGATTGGGTTAATTATTAGAAACATGAAGGTTTTGAAATTGTTAAAGAGATAACCTCCTCTTGAGGGATTTATAGAATCCTTCTTGGGATAAAACTATCTTTTCTTTCAAACTACCCAATTTGATTTTATGCTTTTAAAAAAAGTTACAAATTTCCATGGTTTTAAAAGTTGGAAGTTTAATAATTGTTACGCCTCGCTAAAAAATCTTTTAATAAACCGTCTGCAATCAAAACTATTTATGGAAGTTCTATTAAAACTATATGAAGATCACCCGCCAAGTTTCAATTAAAATCATAGAGAATAAATTCTAACTTTTGTAAGTGGTAGAGAACAAAATTACAATTTAACCTTAATTTAATTTTTAATTTTGATTTTAAATGTTGTGAAAAACATCCATCAAGGTGGAGTACATATATTGGTTCTTATCATAAAACTAGGTTTTAATCATATCGGTCTAGATGAAAATGTTGAAGTTTCAAATAATGAAATGTCAATCTTGAAAAAGATTTTTGTTAAACTAATAAATAAATCCATTTAGTTCAGTAAATTCTTATTTTATACAAACACGTTAAAATGTCTATTATTTATATTATATGCACATAAGTTATATTTTGTTGCTTATTTCATATCTTTCATAATGTTTTTAGAAAATAACTAAAATGTTGATTCACTGTTTATGTTGTCAAGTTCGTGAATATATGAAAATATTGATAAAAATATTCAACATATATCATACTTATAGGTAAAGAATTAAGGAATTTAGTTCTAAATATTTTATAAACACAATTTGGTCCCTCAGTGTATTAATCCCTCTACATATATAAAAATGCTACACTAAATCTTAATAACATTATATTGGGTAAGGATAAATGATTCAAAAATGTTAAATAACACAGTTGAATTGTGTTACAAACTAAATTTTATACACTGAACTAATTATATAATAAAGTTCAATAACTAAGCATTGTAAATTAAAAAGTTCATGATCTTTATTTGTTATATACTTAAGTTTAAAAACCTAAATTGTCCTTCCAAAGTATAGAAATTGAAGTGTCTTTTTCTTTCTTTATTTCCATTTTCCCAAGAACTTTGGAAAGATGACAAATTTAAATGAGTTTTTTAGTGTTCTAATTTTAAGAGATATTCGTAATCGCTTGATCTAATTGTACATTATTAAATCACTTTGTTTTGATGTACACATCGCTTCATGCTATCTACTTTGAGCTAATAATATTGTTGGGCAGGAAGTGACTTTGGTCTATGACTTCAAAAAAGGTTAAAGTTTAATTGTTATAATTTAATTTATAGGGTTGGACAATAACTCATAAATAATAGTCCCTTTAGTTTCAAAAGAACAATCTACACAGACAACTATATCTAAGAGGCTTTTATGAAATCAAAGATATTAGTAAATTTTAATTATGAATTACAATTATGACTAAAACTTTATTTTCATAAAGCCATCAGAAGTGAAATTTGCAATTTAACCAAATACTTTTTACTCAACAGTTATTTCTAATTGTCTAAACAACCATATTTCCTTTTTTTTCCCCATGATAATATGTATAGAATGTTGAATCCATGGTGTTCTTAACTTCAATACTGTTCGAGGATGAGGATGAGGATGAAGACGAAGATGCCATGGTAGAAGAAGCCATAGAAATCATTACAGACATCAACTCACCCACCATTATCCTCAACAATGTCCTAACTCAAAACCCTCAACTCATCGATACCCTAATCTCCATCATCACTTCAACCAAATCCCCTAAATCCCGAGCTTCCACCATAGCCTTCCTAAGCTCCCTCTACACCATTTCTGACCAAATGCATAAAACCTTCACAAAAGATATCCTCTTCATCCAGCTCACAAGAGCCTTAAAGGATCAAGTCGCCACAAAACCCGCCCTCCAAATCCTCCTCCGCTTGGCCCCATTTGGCCGGAATCGCATCAAGGCTGTAAAACATGGTGTTGTTTTCTGTGTTGTCGAATTGCTTCTCAACTCCACTAATGGAAAAGAATGCGAGCTGGCAATGGCAGTCCTGGATCGGCTTTGTGAATGCGCCGAAGGCCGTGCAGAGCTGCTTCAACATGGGGGTGGGATGGCTGTGGTGGGGAGAAAGATTCTTAGAGTGTCGAATTTGGGCAATGAGAAAGCAGTGAGGATTTTGTATAATGTTTGCAAGAACAATGTTGGGAATTGTGGAGTTGCTGAAGAGATGGTGGAGGTTGGGGTTGTAGGGAAGCTTTGTTTGATGCTTCAAGTTGGTGGAAATTTGAAGACTAAAGAAAGAATTAAGGAGATTCTTCATTTACTTCAATGTGTTTTTAAGGGTACAAAGTGTGTTGTTGTTCCTTCTGGGTTTGATCGATTTTAGTTCATACACATTTTTTGTTCATCGATTGAATTTGTTGATCGTATTTATATTTTGAATCCTCCCTGTACATGTGAATGAAAAAATATTATTCAATAAAATCATCACAATGTTGTTTATTGTTGTCTC

mRNA sequence

ATGGCAGAAATCGAGGTTCCTTCTGACTTTTTGTGCCCCATTTCCCTACAACTCATGAGAGATCCGGTAACGATCTCAACCGGGATCACATACGACCGAGAGAGTATCAAGAAATGGCTCTCATCTTGCAAAAACCAACCCCTGAGCTGTCCGGTGTCCAAGCAGGCTCTAAGCAGTGTGGATTTGACACCGAACCATACGCTGCGACGGGTGATCCAAGGGTGGTGTAGTTTGAATGAAAGACATGGGGTGGAGCAGATCCCAACCCCTGAGGATGAGAGTGATAGAGGAGATGTTGTTAAGTTGATCCTTAAGGAGGCAATGAAAAGTCCAAGAAGCTCAATGCTTGAGTGCTTGAAGAGATTGAAATCAATTGTGGCTGAGAATGAGAGCAACAAAATCTACTTGCAGAATGTTGAATCCATGGTGTTCTTAACTTCAATACTGTTCGAGGATGAGGATGAGGATGAAGACGAAGATGCCATGGTAGAAGAAGCCATAGAAATCATTACAGACATCAACTCACCCACCATTATCCTCAACAATGTCCTAACTCAAAACCCTCAACTCATCGATACCCTAATCTCCATCATCACTTCAACCAAATCCCCTAAATCCCGAGCTTCCACCATAGCCTTCCTAAGCTCCCTCTACACCATTTCTGACCAAATGCATAAAACCTTCACAAAAGATATCCTCTTCATCCAGCTCACAAGAGCCTTAAAGGATCAAGTCGCCACAAAACCCGCCCTCCAAATCCTCCTCCGCTTGGCCCCATTTGGCCGGAATCGCATCAAGGCTGTAAAACATGGTGTTGTTTTCTGTGTTGTCGAATTGCTTCTCAACTCCACTAATGGAAAAGAATGCGAGCTGGCAATGGCAGTCCTGGATCGGCTTTGTGAATGCGCCGAAGGCCGTGCAGAGCTGCTTCAACATGGGGGTGGGATGGCTGTGGTGGGGAGAAAGATTCTTAGAGTGTCGAATTTGGGCAATGAGAAAGCAGTGAGGATTTTGTATAATGTTTGCAAGAACAATGTTGGGAATTGTGGAGTTGCTGAAGAGATGGTGGAGGTTGGGGTTGTAGGGAAGCTTTGTTTGATGCTTCAAGTTGGTGGAAATTTGAAGACTAAAGAAAGAATTAAGGAGATTCTTCATTTACTTCAATGTGTTTTTAAGGGTACAAAGTGTGTTGTTGTTCCTTCTGGGTTTGATCGATTTTAG

Coding sequence (CDS)

ATGGCAGAAATCGAGGTTCCTTCTGACTTTTTGTGCCCCATTTCCCTACAACTCATGAGAGATCCGGTAACGATCTCAACCGGGATCACATACGACCGAGAGAGTATCAAGAAATGGCTCTCATCTTGCAAAAACCAACCCCTGAGCTGTCCGGTGTCCAAGCAGGCTCTAAGCAGTGTGGATTTGACACCGAACCATACGCTGCGACGGGTGATCCAAGGGTGGTGTAGTTTGAATGAAAGACATGGGGTGGAGCAGATCCCAACCCCTGAGGATGAGAGTGATAGAGGAGATGTTGTTAAGTTGATCCTTAAGGAGGCAATGAAAAGTCCAAGAAGCTCAATGCTTGAGTGCTTGAAGAGATTGAAATCAATTGTGGCTGAGAATGAGAGCAACAAAATCTACTTGCAGAATGTTGAATCCATGGTGTTCTTAACTTCAATACTGTTCGAGGATGAGGATGAGGATGAAGACGAAGATGCCATGGTAGAAGAAGCCATAGAAATCATTACAGACATCAACTCACCCACCATTATCCTCAACAATGTCCTAACTCAAAACCCTCAACTCATCGATACCCTAATCTCCATCATCACTTCAACCAAATCCCCTAAATCCCGAGCTTCCACCATAGCCTTCCTAAGCTCCCTCTACACCATTTCTGACCAAATGCATAAAACCTTCACAAAAGATATCCTCTTCATCCAGCTCACAAGAGCCTTAAAGGATCAAGTCGCCACAAAACCCGCCCTCCAAATCCTCCTCCGCTTGGCCCCATTTGGCCGGAATCGCATCAAGGCTGTAAAACATGGTGTTGTTTTCTGTGTTGTCGAATTGCTTCTCAACTCCACTAATGGAAAAGAATGCGAGCTGGCAATGGCAGTCCTGGATCGGCTTTGTGAATGCGCCGAAGGCCGTGCAGAGCTGCTTCAACATGGGGGTGGGATGGCTGTGGTGGGGAGAAAGATTCTTAGAGTGTCGAATTTGGGCAATGAGAAAGCAGTGAGGATTTTGTATAATGTTTGCAAGAACAATGTTGGGAATTGTGGAGTTGCTGAAGAGATGGTGGAGGTTGGGGTTGTAGGGAAGCTTTGTTTGATGCTTCAAGTTGGTGGAAATTTGAAGACTAAAGAAAGAATTAAGGAGATTCTTCATTTACTTCAATGTGTTTTTAAGGGTACAAAGTGTGTTGTTGTTCCTTCTGGGTTTGATCGATTTTAG

Protein sequence

MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSVDLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLKRLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIILNNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRALKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLCECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGVVGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVVVPSGFDRF*
Homology
BLAST of CsaV3_1G043860 vs. NCBI nr
Match: XP_004139144.2 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN66593.1 hypothetical protein Csa_006907 [Cucumis sativus])

HSP 1 Score: 785.4 bits (2027), Expect = 2.3e-223
Identity = 406/406 (100.00%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60
           MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV
Sbjct: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60

Query: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120
           DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK
Sbjct: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120

Query: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180
           RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL
Sbjct: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180

Query: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240
           NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA
Sbjct: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240

Query: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300
           LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC
Sbjct: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300

Query: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360
           ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV
Sbjct: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360

Query: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVVVPSGFDRF 407
           VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVVVPSGFDRF
Sbjct: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVVVPSGFDRF 406

BLAST of CsaV3_1G043860 vs. NCBI nr
Match: XP_008450285.1 (PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis melo])

HSP 1 Score: 717.2 bits (1850), Expect = 7.8e-203
Identity = 372/406 (91.63%), Postives = 386/406 (95.07%), Query Frame = 0

Query: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60
           MAEIEVPSDFLCPISLQ+MRDPVTISTGITYDRE IKKWLSSCKNQPL CPVSKQALSSV
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRECIKKWLSSCKNQPLICPVSKQALSSV 60

Query: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120
           DLTPNHTL RVIQGWCSL+ERHGVEQIPTP+DE DRGD VK ILKEAMKSPRSS LECLK
Sbjct: 61  DLTPNHTLGRVIQGWCSLHERHGVEQIPTPKDEIDRGDAVKSILKEAMKSPRSSRLECLK 120

Query: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180
           RLKSIVAENESNKIYLQNVESMVFLTSILF  EDE EDED MVEEAIEIIT+INSP  IL
Sbjct: 121 RLKSIVAENESNKIYLQNVESMVFLTSILF--EDEGEDEDVMVEEAIEIITNINSPATIL 180

Query: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240
            N+LTQNPQLIDTLISIITSTKSPKSRASTIAFL SLYTISDQ HKTFTKDILFIQLTRA
Sbjct: 181 KNLLTQNPQLIDTLISIITSTKSPKSRASTIAFLCSLYTISDQTHKTFTKDILFIQLTRA 240

Query: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300
           L DQ++TKPALQILLRLAPFGRNRIKA+K+GVVF VVELLLNS+NG+ECELAMAVLDRLC
Sbjct: 241 LMDQISTKPALQILLRLAPFGRNRIKAIKNGVVFHVVELLLNSSNGRECELAMAVLDRLC 300

Query: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360
           ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV
Sbjct: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360

Query: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVVVPSGFDRF 407
           V KLCLMLQVGGNLKT+ER+KEILHLLQCV +GTKCVVVPSGFDRF
Sbjct: 361 VAKLCLMLQVGGNLKTRERVKEILHLLQCVSRGTKCVVVPSGFDRF 404

BLAST of CsaV3_1G043860 vs. NCBI nr
Match: XP_038879749.1 (E3 ubiquitin-protein ligase PUB23-like isoform X1 [Benincasa hispida])

HSP 1 Score: 659.4 bits (1700), Expect = 1.9e-185
Identity = 344/407 (84.52%), Postives = 371/407 (91.15%), Query Frame = 0

Query: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60
           MAEIEVPSDFLCPISLQ+MRDPVTISTGITYDRESI+KWLSSCKN  LSCPV+KQAL  +
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIEKWLSSCKN--LSCPVTKQALGGI 60

Query: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120
           DLTPNHTLRRVIQGWCSLN RHGVEQIPTP  E DRGDVVKLILKEA+K PRSS LECLK
Sbjct: 61  DLTPNHTLRRVIQGWCSLNNRHGVEQIPTPNSEDDRGDVVKLILKEAVKGPRSSRLECLK 120

Query: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180
           RLK IVAE+E NKIY QNVESM  L SIL     EDEDE A+VEE IEII++INS T +L
Sbjct: 121 RLKDIVAESERNKIYFQNVESMECLASIL----KEDEDEAAIVEETIEIISNINSSTALL 180

Query: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240
            ++LTQNP+LIDTLISIIT TK+ KSRA+TIAFLSSLYTISDQ HKTFTK+I+F+QLTRA
Sbjct: 181 KHILTQNPRLIDTLISIITITKTSKSRAATIAFLSSLYTISDQTHKTFTKEIIFVQLTRA 240

Query: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300
           LKDQ+ATKP L+ILL LAPFGRNRIKAVK+G VF VVELLL+S+NG+ECELAMAVLDRLC
Sbjct: 241 LKDQIATKPTLKILLHLAPFGRNRIKAVKNGAVFHVVELLLHSSNGRECELAMAVLDRLC 300

Query: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360
           ECAEGRAELL+HGGGMAVVGRKILRVSNLGNEKAVRI+YNVCKNNVGN GV EEMVEVGV
Sbjct: 301 ECAEGRAELLRHGGGMAVVGRKILRVSNLGNEKAVRIVYNVCKNNVGNLGVVEEMVEVGV 360

Query: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV-VVPSGFDRF 407
           VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV V+PSGFDRF
Sbjct: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVLVIPSGFDRF 401

BLAST of CsaV3_1G043860 vs. NCBI nr
Match: XP_038879750.1 (E3 ubiquitin-protein ligase PUB23-like isoform X2 [Benincasa hispida])

HSP 1 Score: 635.6 bits (1638), Expect = 3.0e-178
Identity = 332/393 (84.48%), Postives = 358/393 (91.09%), Query Frame = 0

Query: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60
           MAEIEVPSDFLCPISLQ+MRDPVTISTGITYDRESI+KWLSSCKN  LSCPV+KQAL  +
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIEKWLSSCKN--LSCPVTKQALGGI 60

Query: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120
           DLTPNHTLRRVIQGWCSLN RHGVEQIPTP  E DRGDVVKLILKEA+K PRSS LECLK
Sbjct: 61  DLTPNHTLRRVIQGWCSLNNRHGVEQIPTPNSEDDRGDVVKLILKEAVKGPRSSRLECLK 120

Query: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180
           RLK IVAE+E NKIY QNVESM  L SIL     EDEDE A+VEE IEII++INS T +L
Sbjct: 121 RLKDIVAESERNKIYFQNVESMECLASIL----KEDEDEAAIVEETIEIISNINSSTALL 180

Query: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240
            ++LTQNP+LIDTLISIIT TK+ KSRA+TIAFLSSLYTISDQ HKTFTK+I+F+QLTRA
Sbjct: 181 KHILTQNPRLIDTLISIITITKTSKSRAATIAFLSSLYTISDQTHKTFTKEIIFVQLTRA 240

Query: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300
           LKDQ+ATKP L+ILL LAPFGRNRIKAVK+G VF VVELLL+S+NG+ECELAMAVLDRLC
Sbjct: 241 LKDQIATKPTLKILLHLAPFGRNRIKAVKNGAVFHVVELLLHSSNGRECELAMAVLDRLC 300

Query: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360
           ECAEGRAELL+HGGGMAVVGRKILRVSNLGNEKAVRI+YNVCKNNVGN GV EEMVEVGV
Sbjct: 301 ECAEGRAELLRHGGGMAVVGRKILRVSNLGNEKAVRIVYNVCKNNVGNLGVVEEMVEVGV 360

Query: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKG 394
           VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKG
Sbjct: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKG 387

BLAST of CsaV3_1G043860 vs. NCBI nr
Match: XP_022987530.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 563.5 bits (1451), Expect = 1.4e-156
Identity = 296/407 (72.73%), Postives = 341/407 (83.78%), Query Frame = 0

Query: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60
           MAEIEVPSDFLCPISLQ+M DPVTISTGITYDRESI+KWL  CK     CPV+KQ L  +
Sbjct: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK----KCPVTKQPLIGI 60

Query: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120
           DLTPNHTLRRVIQGW  +N  HGVE++ +P+ E D G VVKLILKEAM+  RSS LECL 
Sbjct: 61  DLTPNHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRG-RSSRLECLN 120

Query: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180
           +L++ +AE E NKIYLQ+  SM FLTS+L  +  EDE+E+ +VE+AIEII +INSPT +L
Sbjct: 121 KLEAFLAETERNKIYLQDARSMEFLTSML--NNPEDENEEVIVEKAIEIILNINSPTTLL 180

Query: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240
            ++L QN  LID +ISI+  TK+ +SR + IAFLSSLY+ISDQ HK FTKD LFIQL+RA
Sbjct: 181 EHLLIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRA 240

Query: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300
           L DQ+A KPAL+ILL LAPFGRNRIKAVKHGVVF +VELLLNST G+ECELA+  +D LC
Sbjct: 241 LNDQIAIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLC 300

Query: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360
           ECAEGRAELL+HGGGMAVV RKILRVSN  NEKAVRILY+VCKNNVGN GVAEEMVEVG 
Sbjct: 301 ECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGA 360

Query: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV-VVPSGFDRF 407
           VGKLCLMLQ+GG+LKT ERIKEILH L+C+FKGTKCV V+PSGFDRF
Sbjct: 361 VGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

BLAST of CsaV3_1G043860 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 9.0e-77
Identity = 170/403 (42.18%), Postives = 256/403 (63.52%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSVDL 62
           EIE+P  FLCPISL++M+DPV +STGITYDR+SI+KWL + K    SCPV+KQ ++  DL
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKN--SCPVTKQDITDADL 68

Query: 63  TPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLKRL 122
           TPNHTLRR+IQ WC+LN  +GVE+IPTP     + ++ KLI   A  S   + ++CLKRL
Sbjct: 69  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSA--SSHENQVKCLKRL 128

Query: 123 KSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIILNN 182
           + IV+EN +NK  L+      FL +I+      D +  ++ +EA+ ++  + +   +L N
Sbjct: 129 RQIVSENATNKRCLEAAGVPEFLANIV----SNDSENGSLTDEALNLLYHLETSETVLKN 188

Query: 183 VLT--QNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 242
           +L   ++  ++ +L  I+      +SR      L ++  ++D M     K  +F ++ + 
Sbjct: 189 LLNNKKDNNIVKSLTKIM-QRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQI 248

Query: 243 LKDQV---ATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKE---CELAMA 302
           L D++   ATK A+ IL+ + P+GRNR KAV+ GV+  ++ELL++ +   E    E+AM 
Sbjct: 249 LDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMV 308

Query: 303 VLDRLCECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEE 362
           VLD LC+CAEGRAE L HG  +AVV +KILRVS   +++AVR+L +V +       +  E
Sbjct: 309 VLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGR-FCATPALLHE 368

Query: 363 MVEVGVVGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV 398
           M+++GVV KLCL+LQV    KTKE+ KE+L L   V+K + C+
Sbjct: 369 MLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

BLAST of CsaV3_1G043860 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.4e-76
Identity = 179/428 (41.82%), Postives = 262/428 (61.21%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSVDL 62
           EIE+PS FLCPISL +M+DPV +STGITYDRESI+KWL S K    SCPV+KQ ++  DL
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKN--SCPVTKQVITETDL 63

Query: 63  TPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLKRL 122
           TPNHTLRR+IQ WC+LN  +G+E+IPTP+    + ++ KLI KE+  S   + ++CLKRL
Sbjct: 64  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLI-KES-SSSHLNQVKCLKRL 123

Query: 123 KSIVAENESNKIYLQNVESMVFLTSILFED-------------------------EDEDE 182
           + IV+EN +NK  L+  E   FL +I+                            E+  +
Sbjct: 124 RQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFD 183

Query: 183 DEDAMVEEAIEIITDINSPTIILNNVLT--QNPQLIDTLISIITSTKSPKSRASTIAFLS 242
              ++++EA+ ++  +++    L ++L   +   L+ TL  I+      +SRA     L 
Sbjct: 184 SSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIM-QRGIYESRAYAALLLK 243

Query: 243 SLYTISDQMHKTFTKDILFIQLTRALKDQV---ATKPALQILLRLAPFGRNRIKAVKHGV 302
            L  ++D M     +  LF ++ + L DQ+   AT+ A+QIL+   P+GRNR KAV+ G 
Sbjct: 244 KLLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGT 303

Query: 303 VFCVVELLLNSTNGKE---CELAMAVLDRLCECAEGRAELLQHGGGMAVVGRKILRVSNL 362
           +  ++ELL++ T   E    E+AM VLD LC+CAEGRAE L HG  +AVV +KILRVS +
Sbjct: 304 ISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQI 363

Query: 363 GNEKAVRILYNVCKNNVGNCGVAEEMVEVGVVGKLCLMLQVGGNLKTKERIKEILHLLQC 398
            +E+AVR+L +V +       + +EM+++GVV KLCL+LQV    KTKE+ KE+L L   
Sbjct: 364 TSERAVRVLLSVGR-FCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHAR 423

BLAST of CsaV3_1G043860 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.4e-47
Identity = 154/429 (35.90%), Postives = 228/429 (53.15%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALS-SVD 62
           EIE+P+ F+CPISL++M+DPVT  +GITYDR++I KWL        SCPV+KQ L    D
Sbjct: 7   EIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKVP----SCPVTKQPLPLDSD 66

Query: 63  LTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSM--LECL 122
           LTPNH LRR+IQ WC  NE  GV +I TP     + +VV+ I K   K  + ++   E L
Sbjct: 67  LTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEI-KNLKKFGQEALGREETL 126

Query: 123 KRLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAM-----VEEAIEIITDIN 182
           ++L+ +  +  + ++     E  V  + ILF  +   EDED       ++E++ ++  I 
Sbjct: 127 QKLEVLAMDGNNRRLM---CECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIG 186

Query: 183 SPTIILNNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSL--YTISDQMHKT----- 242
            P+     +L +N +++++L  ++   +   S+A TI  L +L  YT S  + +      
Sbjct: 187 IPSNDAKTILMENDRVMESLTWVL-HQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIF 246

Query: 243 -----FTKDILF--------------------------IQLTRALKDQVATKPALQILLR 302
                F KD++                            +L  +L  + A   AL ILL 
Sbjct: 247 KGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE 306

Query: 303 LAPFGRNRIKAVKHGVVFCVVELLLNSTNGKE-CELAMAVLDRLCECAEGRAELLQHGGG 362
            + + RNR   V  G V  ++EL ++ T  K   EL + VL RLC CA GRAE+L H GG
Sbjct: 307 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 366

Query: 363 MAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGVVGKLCLMLQVGGNLK 385
           +AVV +++LRVS   +++A+ IL  V K +  N  V EEMV VG V KLC +L +   L 
Sbjct: 367 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENM-VVEEMVNVGTVEKLCSVLGMDCGLN 425

BLAST of CsaV3_1G043860 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 8.6e-35
Identity = 119/411 (28.95%), Postives = 203/411 (49.39%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSVDL 62
           EI +PS F CPIS +LM+DPV I++GITYDRE+I+KW  S      +CPV+   L+S++ 
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQ---TCPVTNTVLTSLEQ 89

Query: 63  TPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK-- 122
            PNHT+RR+IQGWC  +   G+E+IPTP        V ++  + +  + R     C++  
Sbjct: 90  IPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMV 149

Query: 123 -RLKSIVAENESN-KIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTI 182
            ++  +  E+E N K   +N   +V    + F+   E+ +   ++EE + ++T       
Sbjct: 150 TKMTRLGKESERNRKCVKENGAGLVL--CVCFDAFSENANASLLLEETVSVLT------- 209

Query: 183 ILNNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLT 242
                          ++ I    +S  +  S+   L  L    DQ      K++L + +T
Sbjct: 210 --------------WMLPIGLEGQSKLTTTSSFNRLVELLRNGDQNAAFLIKELLELNVT 269

Query: 243 --RALKDQVATKPALQILLRLAPFGRNRIKAVKH---------------GVVFCVVELLL 302
              AL      + A    +       N + ++ H                +V   VE+L+
Sbjct: 270 HVHALTKINGVQEAFMKSINRDSTCVNSLISIHHMILTNQETVSRFLELDLVNITVEMLV 329

Query: 303 NSTNGKECELAMAVLDRLCECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNV 362
           +S N   CE A+ VL+ +CE  EGR ++ ++   + ++ +KIL++S    +  V +++ V
Sbjct: 330 DSEN-SVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKV 389

Query: 363 CKNNVGNCGVAEEMVEVGVVGKLCLMLQVGGNLKTKERIKEILHLLQCVFK 393
           CK+  G+    EE + +G   KL +MLQVG    TKE++ E+L ++  V K
Sbjct: 390 CKS--GDGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMK 409

BLAST of CsaV3_1G043860 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.5e-34
Identity = 111/398 (27.89%), Postives = 202/398 (50.75%), Query Frame = 0

Query: 2   AEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSVD 61
           +EI +P +F CPIS+ LM+DPV ISTGITYDR SI+ W++S      +CPV+   L++ D
Sbjct: 27  SEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNK---TCPVTNTVLTTFD 86

Query: 62  LTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLEC--- 121
             PNHT+R++IQGWC       +++IPTP       +V ++  K +  + R    +C   
Sbjct: 87  QIPNHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVI 146

Query: 122 LKRLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTI 181
           ++++K +  E+E N+  + N  S+ ++    F+    DE    M+ E + ++T +  P  
Sbjct: 147 IEKIKKLGDESEKNRKCV-NENSVGWVLCDCFDKFSGDEKLTFMLNEILSLLTWM-FPIG 206

Query: 182 ILNNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLT 241
           +       +      +  ++ ST     + +       L     ++H    ++ +   L 
Sbjct: 207 LEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALV 266

Query: 242 RALKDQVATKPALQILLRLAPFGRNRIKAVKH----GVVFCVVELLLNSTNGKECELAMA 301
           + ++D V++      L+ +      + +        G+V   VE+++++ N   CE A+A
Sbjct: 267 KLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAEN-SVCEKALA 326

Query: 302 VLDRLCECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEE 361
           VLD +CE   GR E+ ++   M ++ +KI +VS L    ++ ++  + K   GN    E+
Sbjct: 327 VLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWK--TGNTVAVED 386

Query: 362 MVEVGVVGKLCLMLQVGGNLKTKERIKEILHLLQCVFK 393
            V +G   K+ L+LQVG   +TKE+  E+L ++    K
Sbjct: 387 AVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMK 416

BLAST of CsaV3_1G043860 vs. ExPASy TrEMBL
Match: A0A0A0LXW1 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G635930 PE=4 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.1e-223
Identity = 406/406 (100.00%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60
           MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV
Sbjct: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60

Query: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120
           DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK
Sbjct: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120

Query: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180
           RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL
Sbjct: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180

Query: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240
           NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA
Sbjct: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240

Query: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300
           LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC
Sbjct: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300

Query: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360
           ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV
Sbjct: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360

Query: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVVVPSGFDRF 407
           VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVVVPSGFDRF
Sbjct: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVVVPSGFDRF 406

BLAST of CsaV3_1G043860 vs. ExPASy TrEMBL
Match: A0A1S3BNV9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491935 PE=4 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 3.8e-203
Identity = 372/406 (91.63%), Postives = 386/406 (95.07%), Query Frame = 0

Query: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60
           MAEIEVPSDFLCPISLQ+MRDPVTISTGITYDRE IKKWLSSCKNQPL CPVSKQALSSV
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRECIKKWLSSCKNQPLICPVSKQALSSV 60

Query: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120
           DLTPNHTL RVIQGWCSL+ERHGVEQIPTP+DE DRGD VK ILKEAMKSPRSS LECLK
Sbjct: 61  DLTPNHTLGRVIQGWCSLHERHGVEQIPTPKDEIDRGDAVKSILKEAMKSPRSSRLECLK 120

Query: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180
           RLKSIVAENESNKIYLQNVESMVFLTSILF  EDE EDED MVEEAIEIIT+INSP  IL
Sbjct: 121 RLKSIVAENESNKIYLQNVESMVFLTSILF--EDEGEDEDVMVEEAIEIITNINSPATIL 180

Query: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240
            N+LTQNPQLIDTLISIITSTKSPKSRASTIAFL SLYTISDQ HKTFTKDILFIQLTRA
Sbjct: 181 KNLLTQNPQLIDTLISIITSTKSPKSRASTIAFLCSLYTISDQTHKTFTKDILFIQLTRA 240

Query: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300
           L DQ++TKPALQILLRLAPFGRNRIKA+K+GVVF VVELLLNS+NG+ECELAMAVLDRLC
Sbjct: 241 LMDQISTKPALQILLRLAPFGRNRIKAIKNGVVFHVVELLLNSSNGRECELAMAVLDRLC 300

Query: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360
           ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV
Sbjct: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360

Query: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVVVPSGFDRF 407
           V KLCLMLQVGGNLKT+ER+KEILHLLQCV +GTKCVVVPSGFDRF
Sbjct: 361 VAKLCLMLQVGGNLKTRERVKEILHLLQCVSRGTKCVVVPSGFDRF 404

BLAST of CsaV3_1G043860 vs. ExPASy TrEMBL
Match: A0A6J1JH48 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485066 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 7.0e-157
Identity = 296/407 (72.73%), Postives = 341/407 (83.78%), Query Frame = 0

Query: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60
           MAEIEVPSDFLCPISLQ+M DPVTISTGITYDRESI+KWL  CK     CPV+KQ L  +
Sbjct: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK----KCPVTKQPLIGI 60

Query: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120
           DLTPNHTLRRVIQGW  +N  HGVE++ +P+ E D G VVKLILKEAM+  RSS LECL 
Sbjct: 61  DLTPNHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRG-RSSRLECLN 120

Query: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180
           +L++ +AE E NKIYLQ+  SM FLTS+L  +  EDE+E+ +VE+AIEII +INSPT +L
Sbjct: 121 KLEAFLAETERNKIYLQDARSMEFLTSML--NNPEDENEEVIVEKAIEIILNINSPTTLL 180

Query: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240
            ++L QN  LID +ISI+  TK+ +SR + IAFLSSLY+ISDQ HK FTKD LFIQL+RA
Sbjct: 181 EHLLIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRA 240

Query: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300
           L DQ+A KPAL+ILL LAPFGRNRIKAVKHGVVF +VELLLNST G+ECELA+  +D LC
Sbjct: 241 LNDQIAIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLC 300

Query: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360
           ECAEGRAELL+HGGGMAVV RKILRVSN  NEKAVRILY+VCKNNVGN GVAEEMVEVG 
Sbjct: 301 ECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGA 360

Query: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV-VVPSGFDRF 407
           VGKLCLMLQ+GG+LKT ERIKEILH L+C+FKGTKCV V+PSGFDRF
Sbjct: 361 VGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

BLAST of CsaV3_1G043860 vs. ExPASy TrEMBL
Match: A0A6J1HD28 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461732 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.2e-156
Identity = 294/407 (72.24%), Postives = 341/407 (83.78%), Query Frame = 0

Query: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60
           M EIEVPSDFLCPISLQ+M DPVTISTGITYDRESI+KWL  CKN    CPV+KQ L   
Sbjct: 1   MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKN----CPVTKQPLIGT 60

Query: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120
           DLTPNHTLRRVIQGWC +N  HG E++ +P+ E D G VVK+ILKEAM+  RSS LECL 
Sbjct: 61  DLTPNHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRG-RSSRLECLN 120

Query: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180
           +L++ +AE E NKIYLQ+  SM FL S+L  +  ED +ED +VE+AIEII++INSPT +L
Sbjct: 121 KLEAFLAETERNKIYLQDARSMEFLASML--NNPEDGNEDVIVEKAIEIISNINSPTTLL 180

Query: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240
            ++L +N  LID +ISI+  TK+ +SRA+ IAFLSSLY+ISDQ HKTFTKD LFIQL+RA
Sbjct: 181 EHLLIRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRA 240

Query: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300
           L DQ+A KP L+ILL LAPFGRNRIKAVKHGVVF +VELLLNST G+ECELA+  +D+LC
Sbjct: 241 LNDQIAIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLC 300

Query: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360
           ECAEGRAELL+HGGGMAVV RKILRVSN  NEKAVRILY+VCKNNVGN GVAEEMVEVG 
Sbjct: 301 ECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGA 360

Query: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV-VVPSGFDRF 407
           VGKLCLMLQ+GG+LKT ERIKEILH L+C+FKGTKCV V+PSGFDRF
Sbjct: 361 VGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

BLAST of CsaV3_1G043860 vs. ExPASy TrEMBL
Match: A0A6J1EH89 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434282 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.5e-146
Identity = 287/406 (70.69%), Postives = 331/406 (81.53%), Query Frame = 0

Query: 4   IEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPL---SCPVSKQALSSV 63
           +EVPSDFLCPISLQ+MRDPVTISTGITYDRESI+KWLSS KN+ +   SCPV+K  LS +
Sbjct: 3   VEVPSDFLCPISLQIMRDPVTISTGITYDRESIEKWLSSYKNRHVVVPSCPVTKLPLSEL 62

Query: 64  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 123
           DLTPNHTLRR+IQGWCSLN   GVE IPTP+ E DRG VVKL+ KEAM+   SS LECLK
Sbjct: 63  DLTPNHTLRRLIQGWCSLNAGDGVEPIPTPKGEVDRGQVVKLV-KEAMRC-GSSRLECLK 122

Query: 124 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 183
           RLK+IV ENE NK YLQ VESM FLTS+L      + ++++M+EEAIEI+++ NSPT +L
Sbjct: 123 RLKAIVVENERNKFYLQEVESMEFLTSVL------NNNDESMIEEAIEILSNTNSPTTLL 182

Query: 184 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 243
            ++LTQNP LI TLISIIT TKS  S A+ IAFLSSLYTISDQ HKTFT+D LFI LTRA
Sbjct: 183 KHLLTQNPNLIQTLISIITITKSSISSAAAIAFLSSLYTISDQTHKTFTQDTLFIHLTRA 242

Query: 244 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 303
           L DQ+AT P+LQILL LAP GRNR KAVKHG VF +++LL  S+  +ECELAMA LDRLC
Sbjct: 243 LSDQIATNPSLQILLHLAPHGRNRFKAVKHGAVFHLIQLLFGSSIPRECELAMATLDRLC 302

Query: 304 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 363
           ECAEGR ELL+HGGG+AVV RKILRVSNL NEKAVRILYNVCKNNVGN  V EEMVEVG 
Sbjct: 303 ECAEGRVELLRHGGGVAVVARKILRVSNLTNEKAVRILYNVCKNNVGNFRVFEEMVEVGA 362

Query: 364 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKG-TKCV-VVPSGFD 405
           VGKLC +++VGG+LK  ER+K ILHL+Q  F G +KCV V+P  FD
Sbjct: 363 VGKLCAVIEVGGSLKIIERVKVILHLIQRAFDGSSKCVIVIPPRFD 400

BLAST of CsaV3_1G043860 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 288.9 bits (738), Expect = 6.4e-78
Identity = 170/403 (42.18%), Postives = 256/403 (63.52%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSVDL 62
           EIE+P  FLCPISL++M+DPV +STGITYDR+SI+KWL + K    SCPV+KQ ++  DL
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKN--SCPVTKQDITDADL 68

Query: 63  TPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLKRL 122
           TPNHTLRR+IQ WC+LN  +GVE+IPTP     + ++ KLI   A  S   + ++CLKRL
Sbjct: 69  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSA--SSHENQVKCLKRL 128

Query: 123 KSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIILNN 182
           + IV+EN +NK  L+      FL +I+      D +  ++ +EA+ ++  + +   +L N
Sbjct: 129 RQIVSENATNKRCLEAAGVPEFLANIV----SNDSENGSLTDEALNLLYHLETSETVLKN 188

Query: 183 VLT--QNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 242
           +L   ++  ++ +L  I+      +SR      L ++  ++D M     K  +F ++ + 
Sbjct: 189 LLNNKKDNNIVKSLTKIM-QRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQI 248

Query: 243 LKDQV---ATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKE---CELAMA 302
           L D++   ATK A+ IL+ + P+GRNR KAV+ GV+  ++ELL++ +   E    E+AM 
Sbjct: 249 LDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMV 308

Query: 303 VLDRLCECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEE 362
           VLD LC+CAEGRAE L HG  +AVV +KILRVS   +++AVR+L +V +       +  E
Sbjct: 309 VLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGR-FCATPALLHE 368

Query: 363 MVEVGVVGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV 398
           M+++GVV KLCL+LQV    KTKE+ KE+L L   V+K + C+
Sbjct: 369 MLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

BLAST of CsaV3_1G043860 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 287.0 bits (733), Expect = 2.4e-77
Identity = 179/428 (41.82%), Postives = 262/428 (61.21%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSVDL 62
           EIE+PS FLCPISL +M+DPV +STGITYDRESI+KWL S K    SCPV+KQ ++  DL
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKN--SCPVTKQVITETDL 63

Query: 63  TPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLKRL 122
           TPNHTLRR+IQ WC+LN  +G+E+IPTP+    + ++ KLI KE+  S   + ++CLKRL
Sbjct: 64  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLI-KES-SSSHLNQVKCLKRL 123

Query: 123 KSIVAENESNKIYLQNVESMVFLTSILFED-------------------------EDEDE 182
           + IV+EN +NK  L+  E   FL +I+                            E+  +
Sbjct: 124 RQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFD 183

Query: 183 DEDAMVEEAIEIITDINSPTIILNNVLT--QNPQLIDTLISIITSTKSPKSRASTIAFLS 242
              ++++EA+ ++  +++    L ++L   +   L+ TL  I+      +SRA     L 
Sbjct: 184 SSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIM-QRGIYESRAYAALLLK 243

Query: 243 SLYTISDQMHKTFTKDILFIQLTRALKDQV---ATKPALQILLRLAPFGRNRIKAVKHGV 302
            L  ++D M     +  LF ++ + L DQ+   AT+ A+QIL+   P+GRNR KAV+ G 
Sbjct: 244 KLLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGT 303

Query: 303 VFCVVELLLNSTNGKE---CELAMAVLDRLCECAEGRAELLQHGGGMAVVGRKILRVSNL 362
           +  ++ELL++ T   E    E+AM VLD LC+CAEGRAE L HG  +AVV +KILRVS +
Sbjct: 304 ISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQI 363

Query: 363 GNEKAVRILYNVCKNNVGNCGVAEEMVEVGVVGKLCLMLQVGGNLKTKERIKEILHLLQC 398
            +E+AVR+L +V +       + +EM+++GVV KLCL+LQV    KTKE+ KE+L L   
Sbjct: 364 TSERAVRVLLSVGR-FCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHAR 423

BLAST of CsaV3_1G043860 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 190.3 bits (482), Expect = 3.1e-48
Identity = 154/429 (35.90%), Postives = 228/429 (53.15%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALS-SVD 62
           EIE+P+ F+CPISL++M+DPVT  +GITYDR++I KWL        SCPV+KQ L    D
Sbjct: 21  EIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKVP----SCPVTKQPLPLDSD 80

Query: 63  LTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSM--LECL 122
           LTPNH LRR+IQ WC  NE  GV +I TP     + +VV+ I K   K  + ++   E L
Sbjct: 81  LTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEI-KNLKKFGQEALGREETL 140

Query: 123 KRLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAM-----VEEAIEIITDIN 182
           ++L+ +  +  + ++     E  V  + ILF  +   EDED       ++E++ ++  I 
Sbjct: 141 QKLEVLAMDGNNRRLM---CECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIG 200

Query: 183 SPTIILNNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSL--YTISDQMHKT----- 242
            P+     +L +N +++++L  ++   +   S+A TI  L +L  YT S  + +      
Sbjct: 201 IPSNDAKTILMENDRVMESLTWVL-HQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIF 260

Query: 243 -----FTKDILF--------------------------IQLTRALKDQVATKPALQILLR 302
                F KD++                            +L  +L  + A   AL ILL 
Sbjct: 261 KGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE 320

Query: 303 LAPFGRNRIKAVKHGVVFCVVELLLNSTNGKE-CELAMAVLDRLCECAEGRAELLQHGGG 362
            + + RNR   V  G V  ++EL ++ T  K   EL + VL RLC CA GRAE+L H GG
Sbjct: 321 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 380

Query: 363 MAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGVVGKLCLMLQVGGNLK 385
           +AVV +++LRVS   +++A+ IL  V K +  N  V EEMV VG V KLC +L +   L 
Sbjct: 381 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENM-VVEEMVNVGTVEKLCSVLGMDCGLN 439

BLAST of CsaV3_1G043860 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 149.4 bits (376), Expect = 6.1e-36
Identity = 119/411 (28.95%), Postives = 203/411 (49.39%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSVDL 62
           EI +PS F CPIS +LM+DPV I++GITYDRE+I+KW  S      +CPV+   L+S++ 
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQ---TCPVTNTVLTSLEQ 89

Query: 63  TPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK-- 122
            PNHT+RR+IQGWC  +   G+E+IPTP        V ++  + +  + R     C++  
Sbjct: 90  IPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMV 149

Query: 123 -RLKSIVAENESN-KIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTI 182
            ++  +  E+E N K   +N   +V    + F+   E+ +   ++EE + ++T       
Sbjct: 150 TKMTRLGKESERNRKCVKENGAGLVL--CVCFDAFSENANASLLLEETVSVLT------- 209

Query: 183 ILNNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLT 242
                          ++ I    +S  +  S+   L  L    DQ      K++L + +T
Sbjct: 210 --------------WMLPIGLEGQSKLTTTSSFNRLVELLRNGDQNAAFLIKELLELNVT 269

Query: 243 --RALKDQVATKPALQILLRLAPFGRNRIKAVKH---------------GVVFCVVELLL 302
              AL      + A    +       N + ++ H                +V   VE+L+
Sbjct: 270 HVHALTKINGVQEAFMKSINRDSTCVNSLISIHHMILTNQETVSRFLELDLVNITVEMLV 329

Query: 303 NSTNGKECELAMAVLDRLCECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNV 362
           +S N   CE A+ VL+ +CE  EGR ++ ++   + ++ +KIL++S    +  V +++ V
Sbjct: 330 DSEN-SVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKV 389

Query: 363 CKNNVGNCGVAEEMVEVGVVGKLCLMLQVGGNLKTKERIKEILHLLQCVFK 393
           CK+  G+    EE + +G   KL +MLQVG    TKE++ E+L ++  V K
Sbjct: 390 CKS--GDGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMK 409

BLAST of CsaV3_1G043860 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 148.7 bits (374), Expect = 1.0e-35
Identity = 111/398 (27.89%), Postives = 202/398 (50.75%), Query Frame = 0

Query: 2   AEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSVD 61
           +EI +P +F CPIS+ LM+DPV ISTGITYDR SI+ W++S      +CPV+   L++ D
Sbjct: 27  SEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNK---TCPVTNTVLTTFD 86

Query: 62  LTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLEC--- 121
             PNHT+R++IQGWC       +++IPTP       +V ++  K +  + R    +C   
Sbjct: 87  QIPNHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVI 146

Query: 122 LKRLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTI 181
           ++++K +  E+E N+  + N  S+ ++    F+    DE    M+ E + ++T +  P  
Sbjct: 147 IEKIKKLGDESEKNRKCV-NENSVGWVLCDCFDKFSGDEKLTFMLNEILSLLTWM-FPIG 206

Query: 182 ILNNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLT 241
           +       +      +  ++ ST     + +       L     ++H    ++ +   L 
Sbjct: 207 LEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALV 266

Query: 242 RALKDQVATKPALQILLRLAPFGRNRIKAVKH----GVVFCVVELLLNSTNGKECELAMA 301
           + ++D V++      L+ +      + +        G+V   VE+++++ N   CE A+A
Sbjct: 267 KLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAEN-SVCEKALA 326

Query: 302 VLDRLCECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEE 361
           VLD +CE   GR E+ ++   M ++ +KI +VS L    ++ ++  + K   GN    E+
Sbjct: 327 VLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWK--TGNTVAVED 386

Query: 362 MVEVGVVGKLCLMLQVGGNLKTKERIKEILHLLQCVFK 393
            V +G   K+ L+LQVG   +TKE+  E+L ++    K
Sbjct: 387 AVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMK 416

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004139144.22.3e-223100.00E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN66593.1 hypothetical pro... [more]
XP_008450285.17.8e-20391.63PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis melo][more]
XP_038879749.11.9e-18584.52E3 ubiquitin-protein ligase PUB23-like isoform X1 [Benincasa hispida][more]
XP_038879750.13.0e-17884.48E3 ubiquitin-protein ligase PUB23-like isoform X2 [Benincasa hispida][more]
XP_022987530.11.4e-15672.73E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q84TG39.0e-7742.18E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC63.4e-7641.82E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF154.4e-4735.90E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9C8D18.6e-3528.95U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1... [more]
Q5PNY61.5e-3427.89U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0LXW11.1e-223100.00RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G635930 PE... [more]
A0A1S3BNV93.8e-20391.63RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491935 PE=4 ... [more]
A0A6J1JH487.0e-15772.73RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485066 P... [more]
A0A6J1HD281.2e-15672.24RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461732... [more]
A0A6J1EH891.5e-14670.69RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434282... [more]
Match NameE-valueIdentityDescription
AT2G35930.16.4e-7842.18plant U-box 23 [more]
AT3G52450.12.4e-7741.82plant U-box 22 [more]
AT3G11840.13.1e-4835.90plant U-box 24 [more]
AT1G66160.16.1e-3628.95CYS, MET, PRO, and GLY protein 1 [more]
AT5G37490.11.0e-3527.89ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 9..75
e-value: 7.2E-30
score: 115.2
IPR003613U box domainPFAMPF04564U-boxcoord: 6..79
e-value: 3.0E-18
score: 65.8
IPR003613U box domainPROSITEPS51698U_BOXcoord: 5..82
score: 36.649502
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1..87
e-value: 1.7E-27
score: 97.3
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 96..396
e-value: 1.8E-10
score: 42.0
NoneNo IPR availablePANTHERPTHR22849:SF11OS11G0573500 PROTEINcoord: 5..399
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 5..399
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 10..55
e-value: 8.33042E-21
score: 82.6667
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 4..84
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 113..383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G043860.1CsaV3_1G043860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity