CsaV3_1G040800 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_1G040800 vs. NCBI nr
Match: XP_031738755.1 (mechanosensitive ion channel protein 10 [Cucumis sativus] >KGN66279.1 hypothetical protein Csa_007026 [Cucumis sativus])

HSP 1 Score: 1461.4 bits (3782), Expect = 0.0e+00
Identity = 762/762 (100.00%), Postives = 762/762 (100.00%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120
           SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL
Sbjct: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120

Query: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180
           YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG
Sbjct: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180

Query: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300
           INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540

Query: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAPSQR 763
           SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAPSQR
Sbjct: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAPSQR 762

BLAST of CsaV3_1G040800 vs. NCBI nr
Match: XP_008450905.1 (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >XP_008450906.1 PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >TYK10044.1 mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1406.7 bits (3640), Expect = 0.0e+00
Identity = 734/768 (95.57%), Postives = 749/768 (97.53%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGV  SSKDLNKPSVGSFPDFDLKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120
           SHEISRMTP KPPKIPGETVTRRASFACSSF+KPKSRLIEPPCPDG SLAEEK LAKS+L
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180
           Y S KVDSPAKIT VTSPKEALKA PITPKTPLIGTTGNEEEDDEEVY+TAELKVKEKS 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTVIVEW+AFLCLTGCLIASLTI+TLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVIIFIWLALVLLAWGLLFDQSSKRSK+GN+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSFKHLK+ESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVE+KDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           V+LIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAA------PSQR 763
           SNRRSDLVLELKKIFE+LGIKYHLLPQ VQLNY SSAA      PSQR
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of CsaV3_1G040800 vs. NCBI nr
Match: KAA0055793.1 (mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 733/768 (95.44%), Postives = 749/768 (97.53%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEG EGV  SSKDLNKPSVGSFPDFDLKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGGEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120
           SHEISRMTP KPPKIPGETVTRRASFACSSF+KPKSRLIEPPCPDG SLAEEK LAKS+L
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180
           YSS KVDSPAKIT VTSPKEALKA PITPKTPLIGTTGNEEEDDEEVY+TAELKVKEKS 
Sbjct: 121 YSSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTVIVEW+AFLCLTGCLIASLTI+TLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVIIFIWLALVLLAWGLLFDQSSKRSK+GN+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSFKHLK+ESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVE+KDKEINSEWEARAAAYQIFRNVAKPG+KYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGNKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           V+LIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAA------PSQR 763
           SNRRSDLVLELKKIFE+LGIKYHLLPQ VQLNY SSAA      PSQR
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of CsaV3_1G040800 vs. NCBI nr
Match: XP_038878911.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 1358.2 bits (3514), Expect = 0.0e+00
Identity = 701/758 (92.48%), Postives = 735/758 (96.97%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGV ++SKDL KPSV SFPDFDLKETRSFRCTIPQSVVGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGV-DTSKDLTKPSVSSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120
           SHEISRM+P KPPKIPGET  RR SFA SSF+KPKSRLIE PCPD ASLAEEK  AKSTL
Sbjct: 61  SHEISRMSPLKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDSASLAEEKAKAKSTL 120

Query: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180
           YSS K+DSPAKITTVTSPKEALK+APITPKTPLIGTTG+EEEDDEEVY+TAELKVKE+SG
Sbjct: 121 YSSPKMDSPAKITTVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSG 180

Query: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           K+LK+TV++EW+AFLCLTGCLIASLTI+ LVTKEIWGLGLWKWCVLVLV FCGRLFSQWF
Sbjct: 181 KKLKRTVLIEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVSFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300
           INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWL LVLLAWGLLFDQSSKRSK+GN+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLGLVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NY+TRALGASLIGAGLWLVKTL+VKILAASFQC RFFDRIQESIFHQYILRILSGPP+ME
Sbjct: 301 NYITRALGASLIGAGLWLVKTLMVKILAASFQCARFFDRIQESIFHQYILRILSGPPIME 360

Query: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MAE VGRAASTGQLSF+HLKKESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAESVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENFKEEE E+KDKEINSEWEARAAAYQIFRNVAKPGSKYIDE+DLFRFM
Sbjct: 421 SSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540
           SKEEIDNVLPLFEGG ETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           ++LIVIIIEWLLLMGFLTTQVLVF+SSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 IVLIVIIIEWLLLMGFLTTQVLVFVSSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSI+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIEFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPN+SV+VKEIEN+NKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNHSVIVKEIENINKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAA 759
           S+RRSDLVLELKKIFE+LGIKYHLLPQEVQLNYV SAA
Sbjct: 721 SSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVGSAA 757

BLAST of CsaV3_1G040800 vs. NCBI nr
Match: XP_022987285.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022987286.1 mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 662/763 (86.76%), Postives = 710/763 (93.05%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKG EQ+VLRIL+GEEGV ++   L   SV SFPDF+ KETRS RC IPQSVV SSP
Sbjct: 1   MADKKGTEQVVLRILDGEEGV-DARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSP 60

Query: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKST- 120
           SHEISRM+  KPPKIP E+  RR SFA SSF+KPKSRLIEPPCPDGAS AEE   AKST 
Sbjct: 61  SHEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTS 120

Query: 121 ---LYSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVK 180
                SS K+DSPAKI T TSPKE+LK+APITP+TPL+G+TG+EEEDDEEVY+TAELKVK
Sbjct: 121 GSPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVK 180

Query: 181 EKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLF 240
           EKS K+LK+ V++EWIAFLC+T CLI+SLTI+ L+TKEIWGLGLWKWCVLVLVIFCGRLF
Sbjct: 181 EKSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLF 240

Query: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEG 300
           SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVI+FIWLALVLLAWGLLFDQS KRSK+ 
Sbjct: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKV 300

Query: 301 NKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360
           N+ILNYVTRAL ASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP
Sbjct: 301 NEILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360

Query: 361 PLMEMAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420
           PLMEMAERVGR AS GQLSF+HLKKE D GNEGKEEVIDVDKLKKMKQ K+SAWTMRGLI
Sbjct: 361 PLMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLI 420

Query: 421 NVIRGSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 480
           NVIR SGLSTISNTIENFKEEE E+KDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 540
           FRFM+KEEIDNVLPLFEGG ETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+
Sbjct: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540

Query: 541 LSSAVILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPF 600
           L+SA +LIVIII WLLLMGFLTTQVLVFISSQ+LLVVFMFGNTA+T+FEAIIFVFVMHPF
Sbjct: 541 LASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDF 660
           DVGDRCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSDSIDF
Sbjct: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDF 660

Query: 661 SVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQN 720
           SVDFSTSIESIGALKARIK+YLESKPQFWRPN+SV+VKEIENVNKMKL+L +NHTINFQN
Sbjct: 661 SVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQN 720

Query: 721 YGDKSNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAP 760
           YGD+S+RRSDLVLELKKIFE+LGIKYHLLPQEVQLNYVSS AP
Sbjct: 721 YGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAP 762

BLAST of CsaV3_1G040800 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 4.5e-194
Identity = 371/720 (51.53%), Postives = 512/720 (71.11%), Query Frame = 0

Query: 45  RSFRCTIPQSVVG----SSPSHEISRM--TPHKPPKIPGET---VTRRASFACSSFTKPK 104
           RS     P+S  G     SPS EIS++  +P+KPP+ P +    +T+R SFA S ++KPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 105 SRLIEPPCPDGASLAEE---KELAKSTLYSSSKVDSPAKITTVTSPKEALKAAPITPKTP 164
           SR ++P CP   S+ EE   ++L     +S +  ++ +   +V SP      AP+TP   
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNKSN-RSVGSP------APVTPSKV 147

Query: 165 LIGTTGNEEEDDEEVYRTAELKVKEKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVT 224
           ++     E+++DEE+Y+  ++K+  +   ++    ++E   F+ +   L+ASLTI  L  
Sbjct: 148 VV-----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILSALVASLTINVLKH 207

Query: 225 KEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIF 284
              WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV +F
Sbjct: 208 HTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVF 267

Query: 285 IWLALVLLAWGLLFDQSSKRSKEGNKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQ 344
           IWL L+L+AW LLF+   KRS    K+L  +TR L + L GA  WLVKTLL+KILAA+F 
Sbjct: 268 IWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFN 327

Query: 345 CTRFFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFKHLKKESDDGNEGKEE 404
              FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++
Sbjct: 328 VNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKK 387

Query: 405 VIDVDKLKKMKQEKISAWTMRGLINVIRGSGLSTISNTI-ENFKEEEVEKKDKEINSEWE 464
           VID+ K+ KMK+EK+SAWTMR L+  +R SGLSTIS+T+ E    E  E+ D+EI SE E
Sbjct: 388 VIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEME 447

Query: 465 ARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLV 524
           A AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G AETG+I RK    W+V
Sbjct: 448 ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVV 507

Query: 525 NVYVERKSLAHSLNDTKTAIEELNKLSSAVILIVIIIEWLLLMGFLTTQVLVFISSQILL 584
            VY  R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+Q++ 
Sbjct: 508 KVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVA 567

Query: 585 VVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFY 644
           + F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++Y
Sbjct: 568 LAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYY 627

Query: 645 PNSVLATKPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSV 704
           PN+VLATKPISNY+RSP M ++++FS+ FST +  I  LK RI  YLE  PQ W P +SV
Sbjct: 628 PNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSV 687

Query: 705 VVKEIENVNKMKLALCVNHTINFQNYGDKSNRRSDLVLELKKIFEELGIKYHLLPQEVQL 752
           VVKEIEN+NK+K+AL  +HTI FQ   +++ RR++L L +K++ E+L I Y LLPQ++ L
Sbjct: 688 VVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of CsaV3_1G040800 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 8.7e-174
Identity = 354/756 (46.83%), Postives = 497/756 (65.74%), Query Frame = 0

Query: 13  RILEGEEGV-----RESSKDLN-KPSVG--SFPDFDLKETRSFRCTIPQSVVGSSPSHEI 72
           R+  GEE V     +E SKD    PS    + PD D    +S +   P S+    P+ EI
Sbjct: 5   RVSNGEEVVINVSDKEDSKDPRASPSFNPLASPDSDAGIEKS-KPVPPISI----PTPEI 64

Query: 73  SRM--TPHKPPKIPG-ETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEE------KEL 132
            +   + HKPPKIP  E + RR S + S ++KPKSR  E       S  EE      +E 
Sbjct: 65  YKFSGSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQ 124

Query: 133 AKSTLYSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKV 192
             +  ++    D         SP    K+        L      E +++EE+Y+  +L  
Sbjct: 125 FGAGSFARGSFDR-------ASPNN--KSNRSVASAALSKVAEEEPDENEEIYKKVKLHR 184

Query: 193 KEKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRL 252
            ++SG  +K    +E + F+ + G LI SLTI+ +    IWGL  WKWCVLV+V   G L
Sbjct: 185 VKRSG--MKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGML 244

Query: 253 FSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKE 312
            + WF++ +VF+IE+N+LL++KVLYFV+GL+K+V +FIW +LVL+AW  LFD   KR+++
Sbjct: 245 VTNWFMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRK 304

Query: 313 GNKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSG 372
             + L+++T  + + L+G+ L+LVKT  +K+LA+ F    FF+RIQES+FHQY+L+ LSG
Sbjct: 305 TKRFLDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSG 364

Query: 373 PPLMEMAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGL 432
           PPL+E AE VGR  STG LSF   K    DG    ++VID+ K+ +MKQEK+SAWTMR L
Sbjct: 365 PPLIEEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTMRVL 424

Query: 433 INVIRGSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEED 492
           I  +  SG+STIS+T++    ++ E+ DKEI +E EA AAAY +F NVAKP   YI+E+D
Sbjct: 425 IEAVGTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDD 484

Query: 493 LFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELN 552
           L RFM KEE+D VLPL E  A+TGKI RKT   W+VNVY  RK++ HSLNDTKTA+++L+
Sbjct: 485 LLRFMIKEEVDLVLPLIE-DADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLD 544

Query: 553 KLSSAVILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHP 612
           KL + ++ ++  I W++L+   +T++L+  SSQ L + FM G+T + +FE+ +FVFVMHP
Sbjct: 545 KLITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHP 604

Query: 613 FDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSID 672
           +DVGDRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISN+YRSP+M D +D
Sbjct: 605 YDVGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVD 664

Query: 673 FSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQ 732
           F + FST  E IG LK +I  YL +  Q W P   V+V+ IEN+NK+ L + V HTINFQ
Sbjct: 665 FGIAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQ 724

Query: 733 NYGDKSNRRSDLVLELKKIFEELGIKYHLLPQEVQL 752
            Y +KS RR+ L++ +K+I E+L I Y LLPQ+V L
Sbjct: 725 VYVEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of CsaV3_1G040800 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 4.1e-147
Identity = 302/699 (43.20%), Postives = 435/699 (62.23%), Query Frame = 0

Query: 90  SFTKPKSRLIEPPCPDGASL--AEEKELAKSTLYSSSKVDSPAKITTVTSPKEALKAAPI 149
           S  K KSRL +PP P   ++   E K   +S ++ S  +                     
Sbjct: 190 SRNKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGK------------------- 249

Query: 150 TPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSGKRLKKTVIVEWIAFLCLTGCLIASLTI 209
           +PK    G  G EEE++E+ +   +L  + K  K L   V +EWI+ + +   L+ SLTI
Sbjct: 250 SPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDK-LSFWVFLEWISLVLIVTSLVCSLTI 309

Query: 210 ETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRK 269
             L  K  W L LWKW V VLV+ CGRL S W +  +VFL+E+NF  +++VLYFVYG+RK
Sbjct: 310 HNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRK 369

Query: 270 SVIIFIWLALVLLAWGLLFDQSSKRSKEGNKILNYVTRALGASLIGAGLWLVKTLLVKIL 329
           SV   +WL LVLLAW  LFD+  +R    +  L YVTR L   L+   +WLVKT+LVK+L
Sbjct: 370 SVQNCLWLGLVLLAWHFLFDKKVERETR-STALRYVTRVLVCLLVALIIWLVKTILVKVL 429

Query: 330 AASFQCTRFFDRIQESIFHQYILRILSGPPLMEM---------------------AERVG 389
           A+SF  + +FDRIQES+F QY++  LSGPPLME+                       ++ 
Sbjct: 430 ASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLP 489

Query: 390 RAASTGQLSFKHLKKE-------SDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVI 449
            A      SF  + K        S  G +G  E I +D+LK+M  + +SAW M+ L+N+I
Sbjct: 490 PALKATVKSFMKVGKSPGLNRIGSKRGEDG--EGIRIDQLKRMNTKNVSAWNMKRLMNII 549

Query: 450 RGSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRF 509
               +ST+   +++  +E+ +     I SE+EA+ AA +IF NV +PGS+YI  ED  RF
Sbjct: 550 LKGAISTLDQNMQDTTQEDEDA--THIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRF 609

Query: 510 MSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSS 569
           + +EE +  + LFEG +E+ KI +  LKNW+V  + ER++LA +LNDTKTA++ L+++ +
Sbjct: 610 LCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIIN 669

Query: 570 AVILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVG 629
            VI I+III WLL++G  TT+ L+ +SSQ+LLV F+FGN+ +T+FEAIIF+FVMHPFDVG
Sbjct: 670 VVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVG 729

Query: 630 DRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVD 689
           DRC +DGVQ+VVEEMNILTT+FLRYDN+KI YPNSVL TKPI+NYYRSP+M D+++F V 
Sbjct: 730 DRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVH 789

Query: 690 FSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGD 749
            +T  E I A+K RI +Y+++K  +W P   +V   ++++N +K+A+ + H +N Q+ G+
Sbjct: 790 IATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGE 849

Query: 750 KSNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAA 759
           +  RR  L+ E+ K   EL I+Y L P  + +  +   A
Sbjct: 850 RYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTA 863

BLAST of CsaV3_1G040800 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.7e-145
Identity = 307/757 (40.55%), Postives = 447/757 (59.05%), Query Frame = 0

Query: 40  DLKETRSFRCTIPQSVV-GSSPSHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRL 99
           D    R  R T+  SV  G S   E +R+   +  K      T R         K +SRL
Sbjct: 133 DAIRRRQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCTSNRSTMRTKTLMK--MKTRSRL 192

Query: 100 IEPPCPDGASLAEEKELAKSTLYSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTG 159
           ++PP P    +   +      L                            P TP  G  G
Sbjct: 193 MDPPTPTYPDMVSGRTPRSGNL------------------NPGFSGRNTKPGTPNQG--G 252

Query: 160 NEEEDDEEVYRTAELKVKEKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGL 219
           +++ ++EE   + E   +    +++   VI+EWI  + +   LI SL I  L  K +W L
Sbjct: 253 SKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDL 312

Query: 220 GLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALV 279
            LWKW V+VLV+ CGRL S W +   V+ +E NFL ++KVLYFVYG+RK V   +WL LV
Sbjct: 313 ALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLV 372

Query: 280 LLAWGLLFDQSSKRSKEGNKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFD 339
           L+AW  LFD+  +R    + +L YVT+ L   L+   +WL+KTLLVK+LA+SF  + +FD
Sbjct: 373 LIAWHFLFDKKVEREMR-STVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFD 432

Query: 340 RIQESIFHQYILRILSGPPLMEM---AERVGRAASTGQLSFKHLK--------------- 399
           RIQES+F QY++  LSGPP +E+    E+V     T ++  + L                
Sbjct: 433 RIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTV 492

Query: 400 ------------------KESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRGS 459
                               S    EG EE I +D L++M  + +SAW M+ L+NVI+  
Sbjct: 493 GSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKG 552

Query: 460 GLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSK 519
            LST+   I++   +E + K  +I SE+EA+ AA +IF+NVA+PGS+YI  ED  RF+S+
Sbjct: 553 TLSTLDEQIQDTTTQE-DDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSE 612

Query: 520 EEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSAVI 579
           +E +  + LFEG +E  KI +  LKNW+VN + ER++LA +LNDTKTA+  L+++   ++
Sbjct: 613 DESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLV 672

Query: 580 LIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRC 639
            IVI+I WLL++G  TT+ L+ ISSQ+LLVVF+FGN+ +T+FEA+IFVFVMHPFDVGDRC
Sbjct: 673 SIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRC 732

Query: 640 VVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDFST 699
            +DGVQM+VEEMNILTT+FLR+DN+KI YPNS+L TKPI+NYYRSP+M D+I+F V  +T
Sbjct: 733 EIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIAT 792

Query: 700 SIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDKSN 759
             E   AL+ RI +Y+++K   W P+  +V +++  +N +K+A+   H +N QN G++  
Sbjct: 793 PPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYV 852

BLAST of CsaV3_1G040800 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.8e-142
Identity = 297/696 (42.67%), Postives = 433/696 (62.21%), Query Frame = 0

Query: 93  KPKSRLIEPPCPDGASLAEEKELAKSTLYSSSKVDSPAKITTVTSPKEALKAAPITPKTP 152
           + +SRL +PP P         +L   T          A + +   PK     +    K+P
Sbjct: 172 RTRSRLSDPPTP---------QLPPQT----------ADMKSGRIPKSGQMKSGFFGKSP 231

Query: 153 LIGTTGNEEEDDEEVYRTAELKVKEKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVT 212
              T G EEEDD      AE   +E    +L   +++EW++ + +    + +L I +L  
Sbjct: 232 --KTQGEEEEDDP---FAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRK 291

Query: 213 KEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIF 272
           K++W L LWKW  +VLV+ CGRL S W +  +VF IERNFLL+++VLYFVYG+RK+V   
Sbjct: 292 KKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNC 351

Query: 273 IWLALVLLAWGLLFDQSSKRSKEGN-KILNYVTRALGASLIGAGLWLVKTLLVKILAASF 332
           +WL LVLLAW  LFD+  K +K  N K L  VT+     L+G  LWLVKTLLVK+LA+SF
Sbjct: 352 LWLGLVLLAWHFLFDE--KVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSF 411

Query: 333 QCTRFFDRIQESIFHQYILRILSGPPLMEM------AERVG------------------- 392
             + +FDRIQES+F QY++  LSGPPL+E+       ER+                    
Sbjct: 412 HMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQSGAQ 471

Query: 393 -RAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRGSGLS 452
                TG+  F      +  G  G+ + I +D L K+  + +SAW M+ L+N+IR   L+
Sbjct: 472 KSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLT 531

Query: 453 TISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEI 512
           T+   +++   +  + K  +I SE+EA+ AA +IF NVAKPGSK+I   D+ RF+  +E 
Sbjct: 532 TLDEQLQDPSLD--DDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEA 591

Query: 513 DNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSAVILIV 572
              L LFEG +ET +I + +LKNW+VN + ER++LA +LNDTKTA+  L+K+ + V+ I+
Sbjct: 592 LKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGII 651

Query: 573 IIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVD 632
           I++ WL+++G  +T+ LV +SSQ+++V F+FGN  + VFE+II++FV+HPFDVGDRC +D
Sbjct: 652 ILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEID 711

Query: 633 GVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDFSTSIE 692
           GVQMVVEEMNILTT+FLR+DN+K+ YPNS+L TK I NYYRSP+M D I+FS+  +T  E
Sbjct: 712 GVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAE 771

Query: 693 SIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDKSNRRS 752
            I  +K RI +Y+E K   W P   +V K++E++N +++A+   H +N Q+ G+K  RRS
Sbjct: 772 KIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRS 831

Query: 753 DLVLELKKIFEELGIKYHLLPQEVQLNYV--SSAAP 760
            LV E+ KI  EL I+Y L P ++ +  +  S+A P
Sbjct: 832 QLVEEIAKICRELDIEYRLYPLDINVRNLPTSTALP 839

BLAST of CsaV3_1G040800 vs. ExPASy TrEMBL
Match: A0A0A0LYZ9 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_1G595830 PE=3 SV=1)

HSP 1 Score: 1461.4 bits (3782), Expect = 0.0e+00
Identity = 762/762 (100.00%), Postives = 762/762 (100.00%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120
           SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL
Sbjct: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120

Query: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180
           YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG
Sbjct: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180

Query: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300
           INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540

Query: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAPSQR 763
           SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAPSQR
Sbjct: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAPSQR 762

BLAST of CsaV3_1G040800 vs. ExPASy TrEMBL
Match: A0A1S3BR08 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103492355 PE=3 SV=1)

HSP 1 Score: 1406.7 bits (3640), Expect = 0.0e+00
Identity = 734/768 (95.57%), Postives = 749/768 (97.53%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGV  SSKDLNKPSVGSFPDFDLKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120
           SHEISRMTP KPPKIPGETVTRRASFACSSF+KPKSRLIEPPCPDG SLAEEK LAKS+L
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180
           Y S KVDSPAKIT VTSPKEALKA PITPKTPLIGTTGNEEEDDEEVY+TAELKVKEKS 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTVIVEW+AFLCLTGCLIASLTI+TLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVIIFIWLALVLLAWGLLFDQSSKRSK+GN+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSFKHLK+ESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVE+KDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           V+LIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAA------PSQR 763
           SNRRSDLVLELKKIFE+LGIKYHLLPQ VQLNY SSAA      PSQR
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of CsaV3_1G040800 vs. ExPASy TrEMBL
Match: A0A5D3CFQ7 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001770 PE=3 SV=1)

HSP 1 Score: 1406.7 bits (3640), Expect = 0.0e+00
Identity = 734/768 (95.57%), Postives = 749/768 (97.53%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGV  SSKDLNKPSVGSFPDFDLKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120
           SHEISRMTP KPPKIPGETVTRRASFACSSF+KPKSRLIEPPCPDG SLAEEK LAKS+L
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180
           Y S KVDSPAKIT VTSPKEALKA PITPKTPLIGTTGNEEEDDEEVY+TAELKVKEKS 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTVIVEW+AFLCLTGCLIASLTI+TLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVIIFIWLALVLLAWGLLFDQSSKRSK+GN+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSFKHLK+ESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVE+KDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           V+LIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAA------PSQR 763
           SNRRSDLVLELKKIFE+LGIKYHLLPQ VQLNY SSAA      PSQR
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of CsaV3_1G040800 vs. ExPASy TrEMBL
Match: A0A5A7UIR3 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001820 PE=3 SV=1)

HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 733/768 (95.44%), Postives = 749/768 (97.53%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEG EGV  SSKDLNKPSVGSFPDFDLKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGGEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120
           SHEISRMTP KPPKIPGETVTRRASFACSSF+KPKSRLIEPPCPDG SLAEEK LAKS+L
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180
           YSS KVDSPAKIT VTSPKEALKA PITPKTPLIGTTGNEEEDDEEVY+TAELKVKEKS 
Sbjct: 121 YSSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTVIVEW+AFLCLTGCLIASLTI+TLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVIIFIWLALVLLAWGLLFDQSSKRSK+GN+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSFKHLK+ESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVE+KDKEINSEWEARAAAYQIFRNVAKPG+KYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGNKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           V+LIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAA------PSQR 763
           SNRRSDLVLELKKIFE+LGIKYHLLPQ VQLNY SSAA      PSQR
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of CsaV3_1G040800 vs. ExPASy TrEMBL
Match: A0A6J1JDR1 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111484882 PE=3 SV=1)

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 662/763 (86.76%), Postives = 710/763 (93.05%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKG EQ+VLRIL+GEEGV ++   L   SV SFPDF+ KETRS RC IPQSVV SSP
Sbjct: 1   MADKKGTEQVVLRILDGEEGV-DARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSP 60

Query: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKST- 120
           SHEISRM+  KPPKIP E+  RR SFA SSF+KPKSRLIEPPCPDGAS AEE   AKST 
Sbjct: 61  SHEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTS 120

Query: 121 ---LYSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVK 180
                SS K+DSPAKI T TSPKE+LK+APITP+TPL+G+TG+EEEDDEEVY+TAELKVK
Sbjct: 121 GSPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVK 180

Query: 181 EKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLF 240
           EKS K+LK+ V++EWIAFLC+T CLI+SLTI+ L+TKEIWGLGLWKWCVLVLVIFCGRLF
Sbjct: 181 EKSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLF 240

Query: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEG 300
           SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVI+FIWLALVLLAWGLLFDQS KRSK+ 
Sbjct: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKV 300

Query: 301 NKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360
           N+ILNYVTRAL ASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP
Sbjct: 301 NEILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360

Query: 361 PLMEMAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420
           PLMEMAERVGR AS GQLSF+HLKKE D GNEGKEEVIDVDKLKKMKQ K+SAWTMRGLI
Sbjct: 361 PLMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLI 420

Query: 421 NVIRGSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 480
           NVIR SGLSTISNTIENFKEEE E+KDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 540
           FRFM+KEEIDNVLPLFEGG ETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+
Sbjct: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540

Query: 541 LSSAVILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPF 600
           L+SA +LIVIII WLLLMGFLTTQVLVFISSQ+LLVVFMFGNTA+T+FEAIIFVFVMHPF
Sbjct: 541 LASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDF 660
           DVGDRCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSDSIDF
Sbjct: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDF 660

Query: 661 SVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQN 720
           SVDFSTSIESIGALKARIK+YLESKPQFWRPN+SV+VKEIENVNKMKL+L +NHTINFQN
Sbjct: 661 SVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQN 720

Query: 721 YGDKSNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAP 760
           YGD+S+RRSDLVLELKKIFE+LGIKYHLLPQEVQLNYVSS AP
Sbjct: 721 YGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAP 762

BLAST of CsaV3_1G040800 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 679.5 bits (1752), Expect = 3.2e-195
Identity = 371/720 (51.53%), Postives = 512/720 (71.11%), Query Frame = 0

Query: 45  RSFRCTIPQSVVG----SSPSHEISRM--TPHKPPKIPGET---VTRRASFACSSFTKPK 104
           RS     P+S  G     SPS EIS++  +P+KPP+ P +    +T+R SFA S ++KPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 105 SRLIEPPCPDGASLAEE---KELAKSTLYSSSKVDSPAKITTVTSPKEALKAAPITPKTP 164
           SR ++P CP   S+ EE   ++L     +S +  ++ +   +V SP      AP+TP   
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNKSN-RSVGSP------APVTPSKV 147

Query: 165 LIGTTGNEEEDDEEVYRTAELKVKEKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVT 224
           ++     E+++DEE+Y+  ++K+  +   ++    ++E   F+ +   L+ASLTI  L  
Sbjct: 148 VV-----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILSALVASLTINVLKH 207

Query: 225 KEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIF 284
              WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV +F
Sbjct: 208 HTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVF 267

Query: 285 IWLALVLLAWGLLFDQSSKRSKEGNKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQ 344
           IWL L+L+AW LLF+   KRS    K+L  +TR L + L GA  WLVKTLL+KILAA+F 
Sbjct: 268 IWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFN 327

Query: 345 CTRFFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFKHLKKESDDGNEGKEE 404
              FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++
Sbjct: 328 VNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKK 387

Query: 405 VIDVDKLKKMKQEKISAWTMRGLINVIRGSGLSTISNTI-ENFKEEEVEKKDKEINSEWE 464
           VID+ K+ KMK+EK+SAWTMR L+  +R SGLSTIS+T+ E    E  E+ D+EI SE E
Sbjct: 388 VIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEME 447

Query: 465 ARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLV 524
           A AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G AETG+I RK    W+V
Sbjct: 448 ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVV 507

Query: 525 NVYVERKSLAHSLNDTKTAIEELNKLSSAVILIVIIIEWLLLMGFLTTQVLVFISSQILL 584
            VY  R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+Q++ 
Sbjct: 508 KVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVA 567

Query: 585 VVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFY 644
           + F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++Y
Sbjct: 568 LAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYY 627

Query: 645 PNSVLATKPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSV 704
           PN+VLATKPISNY+RSP M ++++FS+ FST +  I  LK RI  YLE  PQ W P +SV
Sbjct: 628 PNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSV 687

Query: 705 VVKEIENVNKMKLALCVNHTINFQNYGDKSNRRSDLVLELKKIFEELGIKYHLLPQEVQL 752
           VVKEIEN+NK+K+AL  +HTI FQ   +++ RR++L L +K++ E+L I Y LLPQ++ L
Sbjct: 688 VVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of CsaV3_1G040800 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 679.5 bits (1752), Expect = 3.2e-195
Identity = 371/720 (51.53%), Postives = 512/720 (71.11%), Query Frame = 0

Query: 45  RSFRCTIPQSVVG----SSPSHEISRM--TPHKPPKIPGET---VTRRASFACSSFTKPK 104
           RS     P+S  G     SPS EIS++  +P+KPP+ P +    +T+R SFA S ++KPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 105 SRLIEPPCPDGASLAEE---KELAKSTLYSSSKVDSPAKITTVTSPKEALKAAPITPKTP 164
           SR ++P CP   S+ EE   ++L     +S +  ++ +   +V SP      AP+TP   
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNKSN-RSVGSP------APVTPSKV 147

Query: 165 LIGTTGNEEEDDEEVYRTAELKVKEKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVT 224
           ++     E+++DEE+Y+  ++K+  +   ++    ++E   F+ +   L+ASLTI  L  
Sbjct: 148 VV-----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILSALVASLTINVLKH 207

Query: 225 KEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIF 284
              WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV +F
Sbjct: 208 HTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVF 267

Query: 285 IWLALVLLAWGLLFDQSSKRSKEGNKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQ 344
           IWL L+L+AW LLF+   KRS    K+L  +TR L + L GA  WLVKTLL+KILAA+F 
Sbjct: 268 IWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFN 327

Query: 345 CTRFFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFKHLKKESDDGNEGKEE 404
              FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++
Sbjct: 328 VNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKK 387

Query: 405 VIDVDKLKKMKQEKISAWTMRGLINVIRGSGLSTISNTI-ENFKEEEVEKKDKEINSEWE 464
           VID+ K+ KMK+EK+SAWTMR L+  +R SGLSTIS+T+ E    E  E+ D+EI SE E
Sbjct: 388 VIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEME 447

Query: 465 ARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLV 524
           A AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G AETG+I RK    W+V
Sbjct: 448 ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVV 507

Query: 525 NVYVERKSLAHSLNDTKTAIEELNKLSSAVILIVIIIEWLLLMGFLTTQVLVFISSQILL 584
            VY  R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+Q++ 
Sbjct: 508 KVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVA 567

Query: 585 VVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFY 644
           + F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++Y
Sbjct: 568 LAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYY 627

Query: 645 PNSVLATKPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSV 704
           PN+VLATKPISNY+RSP M ++++FS+ FST +  I  LK RI  YLE  PQ W P +SV
Sbjct: 628 PNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSV 687

Query: 705 VVKEIENVNKMKLALCVNHTINFQNYGDKSNRRSDLVLELKKIFEELGIKYHLLPQEVQL 752
           VVKEIEN+NK+K+AL  +HTI FQ   +++ RR++L L +K++ E+L I Y LLPQ++ L
Sbjct: 688 VVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of CsaV3_1G040800 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 679.5 bits (1752), Expect = 3.2e-195
Identity = 371/720 (51.53%), Postives = 512/720 (71.11%), Query Frame = 0

Query: 45  RSFRCTIPQSVVG----SSPSHEISRM--TPHKPPKIPGET---VTRRASFACSSFTKPK 104
           RS     P+S  G     SPS EIS++  +P+KPP+ P +    +T+R SFA S ++KPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 105 SRLIEPPCPDGASLAEE---KELAKSTLYSSSKVDSPAKITTVTSPKEALKAAPITPKTP 164
           SR ++P CP   S+ EE   ++L     +S +  ++ +   +V SP      AP+TP   
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNKSN-RSVGSP------APVTPSKV 147

Query: 165 LIGTTGNEEEDDEEVYRTAELKVKEKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVT 224
           ++     E+++DEE+Y+  ++K+  +   ++    ++E   F+ +   L+ASLTI  L  
Sbjct: 148 VV-----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILSALVASLTINVLKH 207

Query: 225 KEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIF 284
              WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV +F
Sbjct: 208 HTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVF 267

Query: 285 IWLALVLLAWGLLFDQSSKRSKEGNKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQ 344
           IWL L+L+AW LLF+   KRS    K+L  +TR L + L GA  WLVKTLL+KILAA+F 
Sbjct: 268 IWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFN 327

Query: 345 CTRFFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFKHLKKESDDGNEGKEE 404
              FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++
Sbjct: 328 VNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKK 387

Query: 405 VIDVDKLKKMKQEKISAWTMRGLINVIRGSGLSTISNTI-ENFKEEEVEKKDKEINSEWE 464
           VID+ K+ KMK+EK+SAWTMR L+  +R SGLSTIS+T+ E    E  E+ D+EI SE E
Sbjct: 388 VIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEME 447

Query: 465 ARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLV 524
           A AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G AETG+I RK    W+V
Sbjct: 448 ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVV 507

Query: 525 NVYVERKSLAHSLNDTKTAIEELNKLSSAVILIVIIIEWLLLMGFLTTQVLVFISSQILL 584
            VY  R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+Q++ 
Sbjct: 508 KVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVA 567

Query: 585 VVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFY 644
           + F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++Y
Sbjct: 568 LAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYY 627

Query: 645 PNSVLATKPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSV 704
           PN+VLATKPISNY+RSP M ++++FS+ FST +  I  LK RI  YLE  PQ W P +SV
Sbjct: 628 PNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSV 687

Query: 705 VVKEIENVNKMKLALCVNHTINFQNYGDKSNRRSDLVLELKKIFEELGIKYHLLPQEVQL 752
           VVKEIEN+NK+K+AL  +HTI FQ   +++ RR++L L +K++ E+L I Y LLPQ++ L
Sbjct: 688 VVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of CsaV3_1G040800 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 612.1 bits (1577), Expect = 6.2e-175
Identity = 354/756 (46.83%), Postives = 497/756 (65.74%), Query Frame = 0

Query: 13  RILEGEEGV-----RESSKDLN-KPSVG--SFPDFDLKETRSFRCTIPQSVVGSSPSHEI 72
           R+  GEE V     +E SKD    PS    + PD D    +S +   P S+    P+ EI
Sbjct: 5   RVSNGEEVVINVSDKEDSKDPRASPSFNPLASPDSDAGIEKS-KPVPPISI----PTPEI 64

Query: 73  SRM--TPHKPPKIPG-ETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEE------KEL 132
            +   + HKPPKIP  E + RR S + S ++KPKSR  E       S  EE      +E 
Sbjct: 65  YKFSGSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQ 124

Query: 133 AKSTLYSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKV 192
             +  ++    D         SP    K+        L      E +++EE+Y+  +L  
Sbjct: 125 FGAGSFARGSFDR-------ASPNN--KSNRSVASAALSKVAEEEPDENEEIYKKVKLHR 184

Query: 193 KEKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRL 252
            ++SG  +K    +E + F+ + G LI SLTI+ +    IWGL  WKWCVLV+V   G L
Sbjct: 185 VKRSG--MKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGML 244

Query: 253 FSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKE 312
            + WF++ +VF+IE+N+LL++KVLYFV+GL+K+V +FIW +LVL+AW  LFD   KR+++
Sbjct: 245 VTNWFMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRK 304

Query: 313 GNKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSG 372
             + L+++T  + + L+G+ L+LVKT  +K+LA+ F    FF+RIQES+FHQY+L+ LSG
Sbjct: 305 TKRFLDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSG 364

Query: 373 PPLMEMAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGL 432
           PPL+E AE VGR  STG LSF   K    DG    ++VID+ K+ +MKQEK+SAWTMR L
Sbjct: 365 PPLIEEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTMRVL 424

Query: 433 INVIRGSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEED 492
           I  +  SG+STIS+T++    ++ E+ DKEI +E EA AAAY +F NVAKP   YI+E+D
Sbjct: 425 IEAVGTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDD 484

Query: 493 LFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELN 552
           L RFM KEE+D VLPL E  A+TGKI RKT   W+VNVY  RK++ HSLNDTKTA+++L+
Sbjct: 485 LLRFMIKEEVDLVLPLIE-DADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLD 544

Query: 553 KLSSAVILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHP 612
           KL + ++ ++  I W++L+   +T++L+  SSQ L + FM G+T + +FE+ +FVFVMHP
Sbjct: 545 KLITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHP 604

Query: 613 FDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSID 672
           +DVGDRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISN+YRSP+M D +D
Sbjct: 605 YDVGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVD 664

Query: 673 FSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQ 732
           F + FST  E IG LK +I  YL +  Q W P   V+V+ IEN+NK+ L + V HTINFQ
Sbjct: 665 FGIAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQ 724

Query: 733 NYGDKSNRRSDLVLELKKIFEELGIKYHLLPQEVQL 752
            Y +KS RR+ L++ +K+I E+L I Y LLPQ+V L
Sbjct: 725 VYVEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of CsaV3_1G040800 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 523.5 bits (1347), Expect = 2.9e-148
Identity = 302/699 (43.20%), Postives = 435/699 (62.23%), Query Frame = 0

Query: 90  SFTKPKSRLIEPPCPDGASL--AEEKELAKSTLYSSSKVDSPAKITTVTSPKEALKAAPI 149
           S  K KSRL +PP P   ++   E K   +S ++ S  +                     
Sbjct: 190 SRNKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGK------------------- 249

Query: 150 TPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSGKRLKKTVIVEWIAFLCLTGCLIASLTI 209
           +PK    G  G EEE++E+ +   +L  + K  K L   V +EWI+ + +   L+ SLTI
Sbjct: 250 SPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDK-LSFWVFLEWISLVLIVTSLVCSLTI 309

Query: 210 ETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRK 269
             L  K  W L LWKW V VLV+ CGRL S W +  +VFL+E+NF  +++VLYFVYG+RK
Sbjct: 310 HNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRK 369

Query: 270 SVIIFIWLALVLLAWGLLFDQSSKRSKEGNKILNYVTRALGASLIGAGLWLVKTLLVKIL 329
           SV   +WL LVLLAW  LFD+  +R    +  L YVTR L   L+   +WLVKT+LVK+L
Sbjct: 370 SVQNCLWLGLVLLAWHFLFDKKVERETR-STALRYVTRVLVCLLVALIIWLVKTILVKVL 429

Query: 330 AASFQCTRFFDRIQESIFHQYILRILSGPPLMEM---------------------AERVG 389
           A+SF  + +FDRIQES+F QY++  LSGPPLME+                       ++ 
Sbjct: 430 ASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLP 489

Query: 390 RAASTGQLSFKHLKKE-------SDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVI 449
            A      SF  + K        S  G +G  E I +D+LK+M  + +SAW M+ L+N+I
Sbjct: 490 PALKATVKSFMKVGKSPGLNRIGSKRGEDG--EGIRIDQLKRMNTKNVSAWNMKRLMNII 549

Query: 450 RGSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRF 509
               +ST+   +++  +E+ +     I SE+EA+ AA +IF NV +PGS+YI  ED  RF
Sbjct: 550 LKGAISTLDQNMQDTTQEDEDA--THIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRF 609

Query: 510 MSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSS 569
           + +EE +  + LFEG +E+ KI +  LKNW+V  + ER++LA +LNDTKTA++ L+++ +
Sbjct: 610 LCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIIN 669

Query: 570 AVILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVG 629
            VI I+III WLL++G  TT+ L+ +SSQ+LLV F+FGN+ +T+FEAIIF+FVMHPFDVG
Sbjct: 670 VVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVG 729

Query: 630 DRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVD 689
           DRC +DGVQ+VVEEMNILTT+FLRYDN+KI YPNSVL TKPI+NYYRSP+M D+++F V 
Sbjct: 730 DRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVH 789

Query: 690 FSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGD 749
            +T  E I A+K RI +Y+++K  +W P   +V   ++++N +K+A+ + H +N Q+ G+
Sbjct: 790 IATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGE 849

Query: 750 KSNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAA 759
           +  RR  L+ E+ K   EL I+Y L P  + +  +   A
Sbjct: 850 RYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTA 863