CsaV3_1G038620 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_1G038620
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionPectinesterase
Locationchr1: 24347384 .. 24349601 (+)
RNA-Seq ExpressionCsaV3_1G038620
SyntenyCsaV3_1G038620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGGCATATAAGTTAGAAAGAAGGTAAAAAATGTGAAATCATCAAAGTTCACTGCCCTTTACTTTATATCCAATCAATTCCCAGTGCTTTTTCTTGTTATATATCTGCAAAAGTATTTTACTTACGAGGAAAGAAAAAAGGAGCTGATTAAGCCATCTCCTTTCTTCTTTCTACTTTTCAGTCATGTCAAGAGTTTCTTTAGCCATTCATATTTTTATGTTGATCTTTTTGCCTGTGTTACAAGTTTCCTGCTTTGATGTTAATGTTATTTACCCAGATGAACAAAATAATGTTACACAGTCCATGATTTCTCAGATTTGTGCTGATATCGAAGATCGAAATTCATGCATTTCAAACATACAACTTGAGATGGGAAGAAGTGTGAACTCGAATCCAAATTCTGTCTTGAGTGCTGCAATTAGAGCAACTATTAATGAAGCTAGACGAGCCATAGAGTCTATAACAAAGTTCAGCACATTCTCGTTTAGCTACCGTGAAGAAATGGCAATTGAGGACTGCAAGGAGCTTCTTGATTTCTCTGTGGCAGAGCTGGCATGGTCATTAGCTGAGATGAAAAGGATTCGTGCAGGCAAAAACGAAGCTCCCGACGAAGGGAATCTAAAAGCATGGCTAAGCGCCGCTCTCAGCAACCAAGACACATGCCTTGAAGGCTTTGAAGGCACAGACAGGCACATTGTAGGTTTCATTAGAGGTAGTTTGAAGCAAGTTACACTGCTCATTAGCAATGTCTTGGCATTGTACACTCAATTACACAGCTTACCATTTCAACCCCCACGGAATGAAACGATGGAGAAAACTAAAAGTTCGGAGTTTCCAGATTGGATGATGGATAGCGAGCACAAGATTGTGAAATCTCATCCACGTAACGTCCACGTTGATGCAATCGTGGCTTTGGATGGAAGCGGAGATTTCCGTTCTATCACAGAAGCTGTAAATGAAGCTCCAAGTTATAGCAATAGAAGGTACATCATATATGTGAAGAAGGGAGTCTACAAAGAAAATATCGACATGAAGCGAAAGAAAACGAACATAATGTTTATTGGAGATGGTATCGGAGAAACAATAGTGACCGGAAGTCGTAATTTTCTGCAGGGATGGACTACTTTCAGAACTGCAACTGTTGGTGAGTAGTTTAATGTGCATCTCGTTTAGGGAAGTTTTTTGTTTAAATCGTGAAAAAACAAAGGTAGCATCATACATGATGCTGTTTTGACTCGGCTTAAGAATGACATGTTTTCCTATATAAACATTTTGGCTATAGCTGTCTCTGGGAAGGGATTCATAGCAAGAGATATGACATTTCGCAACACGGCTGGACCAGAAAACCATCAGGCAGTGGCACTCCGTGTCGACTCTGATCAGTCAGCCTTCTTCCGGTGCAGCTTCGAAGGTCATCAAGACACCCTTTACGTCCACTCCCTCCGCCAATTTTACAGGGAATGCAACATATACGGCACCATAGACTACATTTTCGGCAACGGGGCAGCAGTATTCCAAAAATGCAACATCTACACCAGAGTTCCTCTCCCACTCCAGAAGGTCACAATCACCGCACAAGGTCGAAAATCTCCACACCAGAGTACAGGTTTTTCAATCCAAGACAGCTTTATTTATGCAACTCAACCAACATATCTTGGAAGACCATGGAAGCTATATTCAAGGACTGTCTTCTTGAACACTTACATGAGTGGTTTGGTACAACCTAGAGGATGGCTTGAGTGGTATGGCAATTTTGCTTTAGGCACATTGTGGTATGGAGAGTATAAAAACTATGGACCTGGTGCATCCCTATCAGGTCGAGTCAAATGGCCTGGGTACCATAATATTCAAGACCCTACCATGGCTAGATTCTTCACCTCCGAGCACTTCATCGACGGTCGAACATGGTTGCCAAAGACGGGTATTAAGTTCACATTGGGATTGAGTAACTAATTAAATATTGTCCTATTATAGGTTTAGGAATTTGAATCCTTGGACATGCTTTTCTTTCCAAATAGTTGGAAATGAGAACTATGTGCAATATCTAAGAATATGATGAATATATCAACAATAAGTTTTCTTACAAGATATGATTTGTGATTCTTGCTTGTTGTTGAAAGATTTAAATTTTGTCTCAATTGACTTTACTTCTACCCTTCTTTTTGCCTTTTTGGCTTTTAGTCTCCAAAGTCTGAAAAACATGGGTTTAGTTTTCAA

mRNA sequence

ATGTCAAGAGTTTCTTTAGCCATTCATATTTTTATGTTGATCTTTTTGCCTGTGTTACAAGTTTCCTGCTTTGATGTTAATGTTATTTACCCAGATGAACAAAATAATGTTACACAGTCCATGATTTCTCAGATTTGTGCTGATATCGAAGATCGAAATTCATGCATTTCAAACATACAACTTGAGATGGGAAGAAGTGTGAACTCGAATCCAAATTCTGTCTTGAGTGCTGCAATTAGAGCAACTATTAATGAAGCTAGACGAGCCATAGAGTCTATAACAAAGTTCAGCACATTCTCGTTTAGCTACCGTGAAGAAATGGCAATTGAGGACTGCAAGGAGCTTCTTGATTTCTCTGTGGCAGAGCTGGCATGGTCATTAGCTGAGATGAAAAGGATTCGTGCAGGCAAAAACGAAGCTCCCGACGAAGGGAATCTAAAAGCATGGCTAAGCGCCGCTCTCAGCAACCAAGACACATGCCTTGAAGGCTTTGAAGGCACAGACAGGCACATTGTAGGTTTCATTAGAGGTAGTTTGAAGCAAGTTACACTGCTCATTAGCAATGTCTTGGCATTGTACACTCAATTACACAGCTTACCATTTCAACCCCCACGGAATGAAACGATGGAGAAAACTAAAAGTTCGGAGTTTCCAGATTGGATGATGGATAGCGAGCACAAGATTGTGAAATCTCATCCACGTAACGTCCACGTTGATGCAATCGTGGCTTTGGATGGAAGCGGAGATTTCCGTTCTATCACAGAAGCTGTAAATGAAGCTCCAAGTTATAGCAATAGAAGGTACATCATATATGTGAAGAAGGGAGTCTACAAAGAAAATATCGACATGAAGCGAAAGAAAACGAACATAATGTTTATTGGAGATGGTATCGGAGAAACAATAGTGACCGGAAGTCGTAATTTTCTGCAGGGATGGACTACTTTCAGAACTGCAACTGTTGCTGTCTCTGGGAAGGGATTCATAGCAAGAGATATGACATTTCGCAACACGGCTGGACCAGAAAACCATCAGGCAGTGGCACTCCGTGTCGACTCTGATCAGTCAGCCTTCTTCCGGTGCAGCTTCGAAGGTCATCAAGACACCCTTTACGTCCACTCCCTCCGCCAATTTTACAGGGAATGCAACATATACGGCACCATAGACTACATTTTCGGCAACGGGGCAGCAGTATTCCAAAAATGCAACATCTACACCAGAGTTCCTCTCCCACTCCAGAAGGTCACAATCACCGCACAAGGTCGAAAATCTCCACACCAGAGTACAGGTTTTTCAATCCAAGACAGCTTTATTTATGCAACTCAACCAACATATCTTGGAAGACCATGGAAGCTATATTCAAGGACTGTCTTCTTGAACACTTACATGAGTGGTTTGGTACAACCTAGAGGATGGCTTGAGTGGTATGGCAATTTTGCTTTAGGCACATTGTGGTATGGAGAGTATAAAAACTATGGACCTGGTGCATCCCTATCAGGTCGAGTCAAATGGCCTGGGTACCATAATATTCAAGACCCTACCATGGCTAGATTCTTCACCTCCGAGCACTTCATCGACGGTCGAACATGGTTGCCAAAGACGGGTATTAAGTTCACATTGGGATTGAGTAACTAA

Coding sequence (CDS)

ATGTCAAGAGTTTCTTTAGCCATTCATATTTTTATGTTGATCTTTTTGCCTGTGTTACAAGTTTCCTGCTTTGATGTTAATGTTATTTACCCAGATGAACAAAATAATGTTACACAGTCCATGATTTCTCAGATTTGTGCTGATATCGAAGATCGAAATTCATGCATTTCAAACATACAACTTGAGATGGGAAGAAGTGTGAACTCGAATCCAAATTCTGTCTTGAGTGCTGCAATTAGAGCAACTATTAATGAAGCTAGACGAGCCATAGAGTCTATAACAAAGTTCAGCACATTCTCGTTTAGCTACCGTGAAGAAATGGCAATTGAGGACTGCAAGGAGCTTCTTGATTTCTCTGTGGCAGAGCTGGCATGGTCATTAGCTGAGATGAAAAGGATTCGTGCAGGCAAAAACGAAGCTCCCGACGAAGGGAATCTAAAAGCATGGCTAAGCGCCGCTCTCAGCAACCAAGACACATGCCTTGAAGGCTTTGAAGGCACAGACAGGCACATTGTAGGTTTCATTAGAGGTAGTTTGAAGCAAGTTACACTGCTCATTAGCAATGTCTTGGCATTGTACACTCAATTACACAGCTTACCATTTCAACCCCCACGGAATGAAACGATGGAGAAAACTAAAAGTTCGGAGTTTCCAGATTGGATGATGGATAGCGAGCACAAGATTGTGAAATCTCATCCACGTAACGTCCACGTTGATGCAATCGTGGCTTTGGATGGAAGCGGAGATTTCCGTTCTATCACAGAAGCTGTAAATGAAGCTCCAAGTTATAGCAATAGAAGGTACATCATATATGTGAAGAAGGGAGTCTACAAAGAAAATATCGACATGAAGCGAAAGAAAACGAACATAATGTTTATTGGAGATGGTATCGGAGAAACAATAGTGACCGGAAGTCGTAATTTTCTGCAGGGATGGACTACTTTCAGAACTGCAACTGTTGCTGTCTCTGGGAAGGGATTCATAGCAAGAGATATGACATTTCGCAACACGGCTGGACCAGAAAACCATCAGGCAGTGGCACTCCGTGTCGACTCTGATCAGTCAGCCTTCTTCCGGTGCAGCTTCGAAGGTCATCAAGACACCCTTTACGTCCACTCCCTCCGCCAATTTTACAGGGAATGCAACATATACGGCACCATAGACTACATTTTCGGCAACGGGGCAGCAGTATTCCAAAAATGCAACATCTACACCAGAGTTCCTCTCCCACTCCAGAAGGTCACAATCACCGCACAAGGTCGAAAATCTCCACACCAGAGTACAGGTTTTTCAATCCAAGACAGCTTTATTTATGCAACTCAACCAACATATCTTGGAAGACCATGGAAGCTATATTCAAGGACTGTCTTCTTGAACACTTACATGAGTGGTTTGGTACAACCTAGAGGATGGCTTGAGTGGTATGGCAATTTTGCTTTAGGCACATTGTGGTATGGAGAGTATAAAAACTATGGACCTGGTGCATCCCTATCAGGTCGAGTCAAATGGCCTGGGTACCATAATATTCAAGACCCTACCATGGCTAGATTCTTCACCTCCGAGCACTTCATCGACGGTCGAACATGGTTGCCAAAGACGGGTATTAAGTTCACATTGGGATTGAGTAACTAA

Protein sequence

MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQLEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSVAELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLKQVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDAIVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGETIVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRCSFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQGRKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFALGTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLGLSN*
Homology
BLAST of CsaV3_1G038620 vs. NCBI nr
Match: XP_004135600.1 (putative pectinesterase/pectinesterase inhibitor 22 isoform X1 [Cucumis sativus] >KGN66043.1 hypothetical protein Csa_007268 [Cucumis sativus])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ
Sbjct: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
           LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV
Sbjct: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180
           AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK
Sbjct: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180

Query: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240
           QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA
Sbjct: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240

Query: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300
           IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET
Sbjct: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300

Query: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360
           IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC
Sbjct: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360

Query: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420
           SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG
Sbjct: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420

Query: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480
           RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL
Sbjct: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480

Query: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540
           GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG
Sbjct: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540

Query: 541 LSN 544
           LSN
Sbjct: 541 LSN 543

BLAST of CsaV3_1G038620 vs. NCBI nr
Match: XP_008450600.1 (PREDICTED: putative pectinesterase/pectinesterase inhibitor 22 isoform X1 [Cucumis melo] >KAA0050921.1 putative pectinesterase/pectinesterase inhibitor 22 isoform X1 [Cucumis melo var. makuwa] >TYK10269.1 putative pectinesterase/pectinesterase inhibitor 22 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 536/543 (98.71%), Postives = 542/543 (99.82%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           MSRVSLAIH+FMLIF+PVLQVSC DVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ
Sbjct: 1   MSRVSLAIHLFMLIFIPVLQVSCIDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
           LEMGRSVNSNP+SVLSAAIRATINEAR+AIESITKFSTFSFSYREEMAIEDCKELLDFSV
Sbjct: 61  LEMGRSVNSNPSSVLSAAIRATINEARQAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180
           AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK
Sbjct: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180

Query: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240
           QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHK+VKSHPRNVHVDA
Sbjct: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKLVKSHPRNVHVDA 240

Query: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300
           IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET
Sbjct: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300

Query: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360
           IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC
Sbjct: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360

Query: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420
           SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG
Sbjct: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420

Query: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480
           RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL
Sbjct: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480

Query: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540
           GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGR+WLPKTGIKFTLG
Sbjct: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRSWLPKTGIKFTLG 540

Query: 541 LSN 544
           LSN
Sbjct: 541 LSN 543

BLAST of CsaV3_1G038620 vs. NCBI nr
Match: XP_038879289.1 (putative pectinesterase/pectinesterase inhibitor 22 [Benincasa hispida])

HSP 1 Score: 1050.0 bits (2714), Expect = 6.8e-303
Identity = 514/543 (94.66%), Postives = 531/543 (97.79%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           M RVSLAIH+FMLIF+P LQVSC D  VIYP+EQNN  QSMISQIC DIE+RNSCISNIQ
Sbjct: 1   MLRVSLAIHLFMLIFIPALQVSCID--VIYPNEQNNGIQSMISQICTDIENRNSCISNIQ 60

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
           LEMGRSVNSNP+SVLSAAIRAT+NEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV
Sbjct: 61  LEMGRSVNSNPSSVLSAAIRATLNEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180
           +ELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK
Sbjct: 121 SELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180

Query: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240
           QVTLLISNVLALYTQLHSLPFQPPRNETMEKT SSEFPDWMMDSEHK+VKSHPRN+HVDA
Sbjct: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTNSSEFPDWMMDSEHKLVKSHPRNIHVDA 240

Query: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300
           +VALDGSGDFRSITEAVNEAP+Y NRRYIIYVKKG+YKENIDMKRKKTNIMFIGDGIGET
Sbjct: 241 VVALDGSGDFRSITEAVNEAPNYGNRRYIIYVKKGIYKENIDMKRKKTNIMFIGDGIGET 300

Query: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360
           IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC
Sbjct: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360

Query: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420
           SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG
Sbjct: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420

Query: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480
           RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL
Sbjct: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480

Query: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540
           GTLWYGEYKNYGPGASLSGRVKWPGYH+I+DP +ARFFTSE FI+GR+WLPKTGIKFTLG
Sbjct: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHDIRDPAVARFFTSERFINGRSWLPKTGIKFTLG 540

Query: 541 LSN 544
           LSN
Sbjct: 541 LSN 541

BLAST of CsaV3_1G038620 vs. NCBI nr
Match: KAG6590455.1 (putative pectinesterase/pectinesterase inhibitor 22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1020.8 bits (2638), Expect = 4.4e-294
Identity = 498/543 (91.71%), Postives = 524/543 (96.50%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           M RVSLAIH+F+ I LP L+VSC D  VIYP+EQNNVTQSMISQIC+D+EDRNSCISNIQ
Sbjct: 1   MLRVSLAIHLFICILLPTLRVSCID--VIYPNEQNNVTQSMISQICSDMEDRNSCISNIQ 60

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
            E+GRSVNSNP+SVLSAAIRAT+NEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV
Sbjct: 61  HELGRSVNSNPSSVLSAAIRATLNEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180
           +ELAWSLAEMKRIR GKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRH+VGFIRGSLK
Sbjct: 121 SELAWSLAEMKRIRTGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHLVGFIRGSLK 180

Query: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240
           QVTLLISNVLALYTQLHS PFQPPRNET+E TKSSEFPDWM D EH++VKSHPR+ HVDA
Sbjct: 181 QVTLLISNVLALYTQLHSSPFQPPRNETIEITKSSEFPDWMTDDEHELVKSHPRSAHVDA 240

Query: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300
           IVALDGSGDFRSITEAVNEAP+YSNRRYIIY+KKGVYKENIDMKRKKTNIMFIGDGIGET
Sbjct: 241 IVALDGSGDFRSITEAVNEAPNYSNRRYIIYIKKGVYKENIDMKRKKTNIMFIGDGIGET 300

Query: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360
           +VTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGP+NHQAVALRVDSDQSAF+RC
Sbjct: 301 VVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPQNHQAVALRVDSDQSAFYRC 360

Query: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420
           SFEG+QDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG
Sbjct: 361 SFEGYQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420

Query: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480
           RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL
Sbjct: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480

Query: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540
           GTLWYGE+KNYGPGASLSGRVKWPGYHNI+D  +ARFFT   FI+GRTWLPKTG+KFTLG
Sbjct: 481 GTLWYGEFKNYGPGASLSGRVKWPGYHNIRDTAVARFFTPMRFINGRTWLPKTGVKFTLG 540

Query: 541 LSN 544
           LSN
Sbjct: 541 LSN 541

BLAST of CsaV3_1G038620 vs. NCBI nr
Match: XP_022961600.1 (putative pectinesterase/pectinesterase inhibitor 22 [Cucurbita moschata])

HSP 1 Score: 1019.6 bits (2635), Expect = 9.9e-294
Identity = 497/543 (91.53%), Postives = 524/543 (96.50%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           M RVS+AIH+F+ I LP L+VSC D  VIYP+EQNNVTQSMISQIC+D+EDRNSCISNIQ
Sbjct: 1   MLRVSVAIHLFICILLPTLRVSCID--VIYPNEQNNVTQSMISQICSDMEDRNSCISNIQ 60

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
            E+GRSVNSNP+SVLSAAIRAT+NEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV
Sbjct: 61  HELGRSVNSNPSSVLSAAIRATLNEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180
           +ELAWSLAEMKRIR GKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRH+VGFIRGSLK
Sbjct: 121 SELAWSLAEMKRIRTGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHLVGFIRGSLK 180

Query: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240
           QVTLLISNVLALYTQLHS PFQPPRNET+E TKSSEFPDWM D EH++VKSHPR+ HVDA
Sbjct: 181 QVTLLISNVLALYTQLHSSPFQPPRNETIEITKSSEFPDWMTDDEHELVKSHPRSAHVDA 240

Query: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300
           IVALDGSGDFRSITEAVNEAP+YSNRRYIIY+KKGVYKENIDMKRKKTNIMFIGDGIGET
Sbjct: 241 IVALDGSGDFRSITEAVNEAPNYSNRRYIIYIKKGVYKENIDMKRKKTNIMFIGDGIGET 300

Query: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360
           +VTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGP+NHQAVALRVDSDQSAF+RC
Sbjct: 301 VVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPQNHQAVALRVDSDQSAFYRC 360

Query: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420
           SFEG+QDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG
Sbjct: 361 SFEGYQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420

Query: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480
           RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL
Sbjct: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480

Query: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540
           GTLWYGE+KNYGPGASLSGRVKWPGYHNI+D  +ARFFT   FI+GRTWLPKTG+KFTLG
Sbjct: 481 GTLWYGEFKNYGPGASLSGRVKWPGYHNIRDTAVARFFTPMRFINGRTWLPKTGVKFTLG 540

Query: 541 LSN 544
           LSN
Sbjct: 541 LSN 541

BLAST of CsaV3_1G038620 vs. ExPASy Swiss-Prot
Match: Q9M9W7 (Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3702 GN=PME22 PE=3 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 1.5e-191
Identity = 330/543 (60.77%), Postives = 417/543 (76.80%), Query Frame = 0

Query: 12  MLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQLEMGRSVNS-N 71
           +L+ L  +  S ++  ++ P +++N  +S++++ C  I+    C+SNI   +  S +  N
Sbjct: 8   LLVMLMSVHTSSYETTILKPYKEDNF-RSLVAKACQFIDAHELCVSNIWTHVKESGHGLN 67

Query: 72  PNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSVAELAWSLAEM 131
           P+SVL AA++   ++A+ A+E I      S   RE++AIEDCKEL+ FSV ELAWS+ EM
Sbjct: 68  PHSVLRAAVKEAHDKAKLAMERIPTVMMLSIRSREQVAIEDCKELVGFSVTELAWSMLEM 127

Query: 132 KRIRAG---------KNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLKQ 191
            ++  G          + A   GNLK WLSAA+SNQDTCLEGFEGT+R     I+GSL+Q
Sbjct: 128 NKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAAMSNQDTCLEGFEGTERKYEELIKGSLRQ 187

Query: 192 VTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSH-PRNVHVDA 251
           VT L+SNVL +YTQL++LPF+  RNE++  +     P+W+ +++  ++  H P  +H + 
Sbjct: 188 VTQLVSNVLDMYTQLNALPFKASRNESVIAS-----PEWLTETDESLMMRHDPSVMHPNT 247

Query: 252 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 311
           +VA+DG G +R+I EA+NEAP++S +RY+IYVKKGVYKENID+K+KKTNIM +GDGIG+T
Sbjct: 248 VVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIGQT 307

Query: 312 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 371
           I+TG RNF+QG TTFRTATVAVSG+GFIA+D+TFRNTAGP+N QAVALRVDSDQSAF+RC
Sbjct: 308 IITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVDSDQSAFYRC 367

Query: 372 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 431
           S EG+QDTLY HSLRQFYR+C IYGTID+IFGNGAAV Q C IYTRVPLPLQKVTITAQG
Sbjct: 368 SVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITAQG 427

Query: 432 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 491
           RKSP+Q+TGF IQ+S++ ATQPTYLGRPWKLYSRTV++NTYMS LVQPRGWLEW+GNFAL
Sbjct: 428 RKSPNQNTGFVIQNSYVLATQPTYLGRPWKLYSRTVYMNTYMSQLVQPRGWLEWFGNFAL 487

Query: 492 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 544
            TLWYGEY N GPG   SGRVKWPGYH I D   A  FT   FIDGR WLP TG+ FT G
Sbjct: 488 DTLWYGEYNNIGPGWRSSGRVKWPGYH-IMDKRTALSFTVGSFIDGRRWLPATGVTFTAG 543

BLAST of CsaV3_1G038620 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.8e-136
Identity = 253/517 (48.94%), Postives = 338/517 (65.38%), Query Frame = 0

Query: 41  MISQICADIEDRNSCISNIQLEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFS 100
           +I  +C+  ++  S    +Q+E  R     P    + A +  ++   +A+  ++KF   +
Sbjct: 17  IIISLCSAHKEAFSSTDLVQMECLRV----PPLEFAEAAKTVVDAITKAVAIVSKFDKKA 76

Query: 101 FSYREEMAIEDCKELLDFSVAELAWSLAEMKRIRAGKNEAPDEG-NLKAWLSAALSNQDT 160
              R   AI DC +LLD +  EL+W ++  +      N   D G +L+ W+SAALSNQDT
Sbjct: 77  GKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDT 136

Query: 161 CLEGFEGTDRHIVGFIRGSLKQVTLLISNVLALYTQLHSLPFQPP----RNETMEKTKS- 220
           CL+GFEGT+  I   + G L +V   + N+L   T +HS P +P     + +TM K  S 
Sbjct: 137 CLDGFEGTNGIIKKIVAGGLSKVGTTVRNLL---TMVHSPPSKPKPKPIKAQTMTKAHSG 196

Query: 221 -SEFPDWMMDSEHKIVKSHPRNVHVDAIVALDGSGDFRSITEAVNEAPSYSNRRYIIYVK 280
            S+FP W+   + K++++    V  DA+VA DG+G+F +I++AV  AP YS +RY+I+VK
Sbjct: 197 FSKFPSWVKPGDRKLLQTDNITV-ADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVK 256

Query: 281 KGVYKENIDMKRKKTNIMFIGDGIGETIVTGSRNFLQGWTTFRTATVAVSGKGFIARDMT 340
           +GVY EN+++K+KK NIM +GDGI  T++TG+R+F+ GWTTFR+AT AVSG+GFIARD+T
Sbjct: 257 RGVYVENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDIT 316

Query: 341 FRNTAGPENHQAVALRVDSDQSAFFRCSFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGN 400
           F+NTAGPE HQAVA+R D+D   F+RC+  G+QDTLY HS+RQF+REC I GT+D+IFG+
Sbjct: 317 FQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGD 376

Query: 401 GAAVFQKCNIYTRVPLPLQKVTITAQGRKSPHQSTGFSIQDSFIYA---------TQPTY 460
             AVFQ C I  +  LP QK +ITAQGRK P++ TGF+IQ S I A         T  TY
Sbjct: 377 ATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATY 436

Query: 461 LGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFALGTLWYGEYKNYGPGASLSGRVKWP 520
           LGRPWKLYSRTVF+  YMS  + P GWLEW GNFAL TL+YGEY N GPGASL  RVKWP
Sbjct: 437 LGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWP 496

Query: 521 GYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLGL 542
           GYH +     A  FT    I G  WLP TGI F  GL
Sbjct: 497 GYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

BLAST of CsaV3_1G038620 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 2.0e-135
Identity = 240/475 (50.53%), Postives = 328/475 (69.05%), Query Frame = 0

Query: 76  SAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSVAELAWSLAEMKRIRA 135
           + +++ TI+  ++    +++F+     +R   AI DC +LLDFS  EL WSL+  +  + 
Sbjct: 57  AGSLKDTIDAVQQVASILSQFANAFGDFRLANAISDCLDLLDFSADELNWSLSASQN-QK 116

Query: 136 GKNEAPDE--GNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLKQVTLLISNVLALY 195
           GKN +  +   +L+ WLSAAL NQDTC  GFEGT+  + G I   L QVT L+  +L   
Sbjct: 117 GKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLVQELL--- 176

Query: 196 TQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDAIVALDGSGDFRSI 255
           TQ+H     P  N   ++  + + P W+   + K++++    V VDAIVA DG+G+F ++
Sbjct: 177 TQVH-----PNSN---QQGPNGQIPSWVKTKDRKLLQAD--GVSVDAIVAQDGTGNFTNV 236

Query: 256 TEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGETIVTGSRNFLQGWT 315
           T+AV  AP YS RRY+IY+K+G YKEN+++K+KK N+M IGDG+  TI++G+R+F+ GWT
Sbjct: 237 TDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWT 296

Query: 316 TFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRCSFEGHQDTLYVHS 375
           TFR+AT AVSG+GFIARD+TF NTAGPE HQAVALR DSD S F+RC+  G+QDTLY H+
Sbjct: 297 TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHT 356

Query: 376 LRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQGRKSPHQSTGFSIQ 435
           +RQFYR+C I GT+D+IFG+   VFQ C I  +  LP QK +ITAQGRK P++ TG SIQ
Sbjct: 357 MRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQ 416

Query: 436 ------DSFIYA----TQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFALGTL 495
                 DS + A    + PTYLGRPWKLYSRTV + +++S +++P GWLEW G+FAL +L
Sbjct: 417 FCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSL 476

Query: 496 WYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFT 539
           +YGEY NYGPGA L  RVKWPGY    + T A+ +T   FI+G  WLP TG+K+T
Sbjct: 477 FYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYT 517

BLAST of CsaV3_1G038620 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 478.4 bits (1230), Expect = 1.1e-133
Identity = 242/495 (48.89%), Postives = 325/495 (65.66%), Query Frame = 0

Query: 71  PNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSVAELAWSLAEM 130
           P S   ++I   +   R+    +++F+ FS   R + A+ DC +LLDFS  EL WS +  
Sbjct: 31  PASEFVSSINTIVVVIRQVSSILSQFADFSGDRRLQNAVSDCLDLLDFSSEELTWSASAS 90

Query: 131 KRIRAGKNEAPDEG-NLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLKQVTLLISNV 190
           +  +   N   D G + + WLSAALSNQ TC+EGF+GT   +   + GSL Q+  ++  +
Sbjct: 91  ENPKGKGNGTGDVGSDTRTWLSAALSNQATCMEGFDGTSGLVKSLVAGSLDQLYSMLREL 150

Query: 191 LALYT------------QLHSLPFQPP--RNETMEKTKSSEFPDWMMDSEHKIVKSHPRN 250
           L L               +   P  PP  +    ++ +S +FPDW+   + K+++S+ R 
Sbjct: 151 LPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLRDTDEDESLQFPDWVRPDDRKLLESNGRT 210

Query: 251 VHVDAIVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGD 310
              D  VALDG+G+F  I +A+ +AP YS+ R++IY+KKG+Y EN+++K+KK NI+ +GD
Sbjct: 211 --YDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGD 270

Query: 311 GIGETIVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQS 370
           GI  T+++G+R+F+ GWTTFR+AT AVSG+GF+ARD+TF+NTAGPE HQAVALR DSD S
Sbjct: 271 GIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLS 330

Query: 371 AFFRCSFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVT 430
            FFRC+  G+QDTLY H++RQFYREC I GT+D+IFG+G  VFQ C I  +  LP QK T
Sbjct: 331 VFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNT 390

Query: 431 ITAQGRKSPHQSTGFSIQDSFIYA---------TQPTYLGRPWKLYSRTVFLNTYMSGLV 490
           ITAQGRK  +Q +GFSIQ S I A         T  TYLGRPWKLYSRTVF+   MS +V
Sbjct: 391 ITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVV 450

Query: 491 QPRGWLEWYGNFALGTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDG 542
           +P GWLEW  +FAL TL+YGE+ NYGPG+ LS RVKWPGYH   +   A  FT   FI G
Sbjct: 451 RPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKG 510

BLAST of CsaV3_1G038620 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 8.3e-118
Identity = 222/518 (42.86%), Postives = 318/518 (61.39%), Query Frame = 0

Query: 42  ISQICADIEDRNSCISNIQLEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSF 101
           IS+ C+     N CI  + L+   S+ ++ N ++  +  AT+ +  +A+ + +  +    
Sbjct: 75  ISRTCSKSLYPNLCIDTL-LDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQM 134

Query: 102 SYREEMAIEDCKELLDFSVAELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCL 161
             R   A + C ELLD SV  L  +L+ +  +   ++ +    ++  WLS+A++N DTC 
Sbjct: 135 PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS----DVMTWLSSAMTNHDTCT 194

Query: 162 EGF---EGTDRHIVGFIRGSLKQVTLLISNVLALYT----QLHSLPFQPPRNETMEKTKS 221
           +GF   EG    +   + G++K ++ ++SN LA++      L  +P    R + +   ++
Sbjct: 195 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNR-KLLGTEET 254

Query: 222 SEFPDWMMDSEHKIVKSHPRNVHVDAIVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKK 281
            E P+W+   + +++ +    +  D  V+ DGSG F++I EA+ +AP +S+RR++IYVK 
Sbjct: 255 EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKA 314

Query: 282 GVY-KENIDMKRKKTNIMFIGDGIGETIVTGSRNFLQGWTTFRTATVAVSGKGFIARDMT 341
           G Y +EN+ + RKKTN+MFIGDG G+T++TG ++     TTF TAT A +G GFI RDMT
Sbjct: 315 GRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMT 374

Query: 342 FRNTAGPENHQAVALRVDSDQSAFFRCSFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGN 401
           F N AGP  HQAVALRV  D +  +RC+  G+QD LYVHS RQF+REC IYGT+D+IFGN
Sbjct: 375 FENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGN 434

Query: 402 GAAVFQKCNIYTRVPLPLQKVTITAQGRKSPHQSTGFSIQDSFIYAT---------QPTY 461
            A + Q CNIY R P+  QK+TITAQ RK P+Q+TG SI    + AT          PTY
Sbjct: 435 AAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTY 494

Query: 462 LGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFALGTLWYGEYKNYGPGASLSGRVKWP 521
           LGRPWKLYSR V++ + M   + PRGWLEW G FAL +L+YGEY N G G+ +  RVKWP
Sbjct: 495 LGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWP 554

Query: 522 GYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLGLS 543
           GYH I     A  FT   FI G +WLP TG+ F  GLS
Sbjct: 555 GYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of CsaV3_1G038620 vs. ExPASy TrEMBL
Match: A0A0A0LWC9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569210 PE=3 SV=1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ
Sbjct: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
           LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV
Sbjct: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180
           AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK
Sbjct: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180

Query: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240
           QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA
Sbjct: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240

Query: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300
           IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET
Sbjct: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300

Query: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360
           IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC
Sbjct: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360

Query: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420
           SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG
Sbjct: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420

Query: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480
           RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL
Sbjct: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480

Query: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540
           GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG
Sbjct: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540

Query: 541 LSN 544
           LSN
Sbjct: 541 LSN 543

BLAST of CsaV3_1G038620 vs. ExPASy TrEMBL
Match: A0A5D3CJ63 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004190 PE=3 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 536/543 (98.71%), Postives = 542/543 (99.82%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           MSRVSLAIH+FMLIF+PVLQVSC DVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ
Sbjct: 1   MSRVSLAIHLFMLIFIPVLQVSCIDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
           LEMGRSVNSNP+SVLSAAIRATINEAR+AIESITKFSTFSFSYREEMAIEDCKELLDFSV
Sbjct: 61  LEMGRSVNSNPSSVLSAAIRATINEARQAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180
           AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK
Sbjct: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180

Query: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240
           QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHK+VKSHPRNVHVDA
Sbjct: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKLVKSHPRNVHVDA 240

Query: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300
           IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET
Sbjct: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300

Query: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360
           IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC
Sbjct: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360

Query: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420
           SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG
Sbjct: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420

Query: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480
           RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL
Sbjct: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480

Query: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540
           GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGR+WLPKTGIKFTLG
Sbjct: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRSWLPKTGIKFTLG 540

Query: 541 LSN 544
           LSN
Sbjct: 541 LSN 543

BLAST of CsaV3_1G038620 vs. ExPASy TrEMBL
Match: A0A1S3BNY2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492140 PE=3 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 536/543 (98.71%), Postives = 542/543 (99.82%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           MSRVSLAIH+FMLIF+PVLQVSC DVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ
Sbjct: 1   MSRVSLAIHLFMLIFIPVLQVSCIDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
           LEMGRSVNSNP+SVLSAAIRATINEAR+AIESITKFSTFSFSYREEMAIEDCKELLDFSV
Sbjct: 61  LEMGRSVNSNPSSVLSAAIRATINEARQAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180
           AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK
Sbjct: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180

Query: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240
           QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHK+VKSHPRNVHVDA
Sbjct: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKLVKSHPRNVHVDA 240

Query: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300
           IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET
Sbjct: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300

Query: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360
           IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC
Sbjct: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360

Query: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420
           SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG
Sbjct: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420

Query: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480
           RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL
Sbjct: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480

Query: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540
           GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGR+WLPKTGIKFTLG
Sbjct: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRSWLPKTGIKFTLG 540

Query: 541 LSN 544
           LSN
Sbjct: 541 LSN 543

BLAST of CsaV3_1G038620 vs. ExPASy TrEMBL
Match: A0A6J1HCP7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111462129 PE=3 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 4.8e-294
Identity = 497/543 (91.53%), Postives = 524/543 (96.50%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           M RVS+AIH+F+ I LP L+VSC D  VIYP+EQNNVTQSMISQIC+D+EDRNSCISNIQ
Sbjct: 1   MLRVSVAIHLFICILLPTLRVSCID--VIYPNEQNNVTQSMISQICSDMEDRNSCISNIQ 60

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
            E+GRSVNSNP+SVLSAAIRAT+NEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV
Sbjct: 61  HELGRSVNSNPSSVLSAAIRATLNEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180
           +ELAWSLAEMKRIR GKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRH+VGFIRGSLK
Sbjct: 121 SELAWSLAEMKRIRTGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHLVGFIRGSLK 180

Query: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240
           QVTLLISNVLALYTQLHS PFQPPRNET+E TKSSEFPDWM D EH++VKSHPR+ HVDA
Sbjct: 181 QVTLLISNVLALYTQLHSSPFQPPRNETIEITKSSEFPDWMTDDEHELVKSHPRSAHVDA 240

Query: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300
           IVALDGSGDFRSITEAVNEAP+YSNRRYIIY+KKGVYKENIDMKRKKTNIMFIGDGIGET
Sbjct: 241 IVALDGSGDFRSITEAVNEAPNYSNRRYIIYIKKGVYKENIDMKRKKTNIMFIGDGIGET 300

Query: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360
           +VTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGP+NHQAVALRVDSDQSAF+RC
Sbjct: 301 VVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPQNHQAVALRVDSDQSAFYRC 360

Query: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420
           SFEG+QDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG
Sbjct: 361 SFEGYQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420

Query: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480
           RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL
Sbjct: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480

Query: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540
           GTLWYGE+KNYGPGASLSGRVKWPGYHNI+D  +ARFFT   FI+GRTWLPKTG+KFTLG
Sbjct: 481 GTLWYGEFKNYGPGASLSGRVKWPGYHNIRDTAVARFFTPMRFINGRTWLPKTGVKFTLG 540

Query: 541 LSN 544
           LSN
Sbjct: 541 LSN 541

BLAST of CsaV3_1G038620 vs. ExPASy TrEMBL
Match: A0A6J1HXZ0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467863 PE=3 SV=1)

HSP 1 Score: 1012.3 bits (2616), Expect = 7.6e-292
Identity = 495/543 (91.16%), Postives = 522/543 (96.13%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           MSRVSLAIH+F+ I LP L+VSC D  VIYP+EQNNVTQSMISQIC+D+EDRNSCISNIQ
Sbjct: 1   MSRVSLAIHLFICILLPTLRVSCID--VIYPNEQNNVTQSMISQICSDMEDRNSCISNIQ 60

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
            E+GRSVNSNP+SVLSAAIRAT+NEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV
Sbjct: 61  HELGRSVNSNPSSVLSAAIRATLNEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLK 180
           +ELAWSLAEMKRIR GKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRH+VGFIRGSLK
Sbjct: 121 SELAWSLAEMKRIRTGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHLVGFIRGSLK 180

Query: 181 QVTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHPRNVHVDA 240
           QVTLLI+NVLALYTQLHS PFQPP NET+E TKSSEFPDWMMD+EH++VKSHPR+ HVDA
Sbjct: 181 QVTLLINNVLALYTQLHSSPFQPPPNETIEITKSSEFPDWMMDNEHELVKSHPRSAHVDA 240

Query: 241 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 300
           IVALDGSGDFRSITEAVNEAP+YS RRYIIY+KKGVYKENIDMKRKKTNIMFIGDGIGET
Sbjct: 241 IVALDGSGDFRSITEAVNEAPNYSYRRYIIYIKKGVYKENIDMKRKKTNIMFIGDGIGET 300

Query: 301 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 360
           +VTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGP+NHQAVALRVDSDQSAF+RC
Sbjct: 301 VVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPQNHQAVALRVDSDQSAFYRC 360

Query: 361 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420
           SFEG+QDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG
Sbjct: 361 SFEGYQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 420

Query: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480
           RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL
Sbjct: 421 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 480

Query: 481 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 540
            TLWYGE+KNYGPGA LSGRVKWPGYHNI+D  +A FFT   FI+GRTWLPKTGIKFTLG
Sbjct: 481 DTLWYGEFKNYGPGAPLSGRVKWPGYHNIRDTAVATFFTPMRFINGRTWLPKTGIKFTLG 540

Query: 541 LSN 544
           LSN
Sbjct: 541 LSN 541

BLAST of CsaV3_1G038620 vs. TAIR 10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 670.6 bits (1729), Expect = 1.0e-192
Identity = 330/543 (60.77%), Postives = 417/543 (76.80%), Query Frame = 0

Query: 12  MLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQLEMGRSVNS-N 71
           +L+ L  +  S ++  ++ P +++N  +S++++ C  I+    C+SNI   +  S +  N
Sbjct: 8   LLVMLMSVHTSSYETTILKPYKEDNF-RSLVAKACQFIDAHELCVSNIWTHVKESGHGLN 67

Query: 72  PNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSVAELAWSLAEM 131
           P+SVL AA++   ++A+ A+E I      S   RE++AIEDCKEL+ FSV ELAWS+ EM
Sbjct: 68  PHSVLRAAVKEAHDKAKLAMERIPTVMMLSIRSREQVAIEDCKELVGFSVTELAWSMLEM 127

Query: 132 KRIRAG---------KNEAPDEGNLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLKQ 191
            ++  G          + A   GNLK WLSAA+SNQDTCLEGFEGT+R     I+GSL+Q
Sbjct: 128 NKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAAMSNQDTCLEGFEGTERKYEELIKGSLRQ 187

Query: 192 VTLLISNVLALYTQLHSLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSH-PRNVHVDA 251
           VT L+SNVL +YTQL++LPF+  RNE++  +     P+W+ +++  ++  H P  +H + 
Sbjct: 188 VTQLVSNVLDMYTQLNALPFKASRNESVIAS-----PEWLTETDESLMMRHDPSVMHPNT 247

Query: 252 IVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGDGIGET 311
           +VA+DG G +R+I EA+NEAP++S +RY+IYVKKGVYKENID+K+KKTNIM +GDGIG+T
Sbjct: 248 VVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIGQT 307

Query: 312 IVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQSAFFRC 371
           I+TG RNF+QG TTFRTATVAVSG+GFIA+D+TFRNTAGP+N QAVALRVDSDQSAF+RC
Sbjct: 308 IITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVDSDQSAFYRC 367

Query: 372 SFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVTITAQG 431
           S EG+QDTLY HSLRQFYR+C IYGTID+IFGNGAAV Q C IYTRVPLPLQKVTITAQG
Sbjct: 368 SVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITAQG 427

Query: 432 RKSPHQSTGFSIQDSFIYATQPTYLGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFAL 491
           RKSP+Q+TGF IQ+S++ ATQPTYLGRPWKLYSRTV++NTYMS LVQPRGWLEW+GNFAL
Sbjct: 428 RKSPNQNTGFVIQNSYVLATQPTYLGRPWKLYSRTVYMNTYMSQLVQPRGWLEWFGNFAL 487

Query: 492 GTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLG 544
            TLWYGEY N GPG   SGRVKWPGYH I D   A  FT   FIDGR WLP TG+ FT G
Sbjct: 488 DTLWYGEYNNIGPGWRSSGRVKWPGYH-IMDKRTALSFTVGSFIDGRRWLPATGVTFTAG 543

BLAST of CsaV3_1G038620 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 487.6 bits (1254), Expect = 1.3e-137
Identity = 253/517 (48.94%), Postives = 338/517 (65.38%), Query Frame = 0

Query: 41  MISQICADIEDRNSCISNIQLEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFS 100
           +I  +C+  ++  S    +Q+E  R     P    + A +  ++   +A+  ++KF   +
Sbjct: 17  IIISLCSAHKEAFSSTDLVQMECLRV----PPLEFAEAAKTVVDAITKAVAIVSKFDKKA 76

Query: 101 FSYREEMAIEDCKELLDFSVAELAWSLAEMKRIRAGKNEAPDEG-NLKAWLSAALSNQDT 160
              R   AI DC +LLD +  EL+W ++  +      N   D G +L+ W+SAALSNQDT
Sbjct: 77  GKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDT 136

Query: 161 CLEGFEGTDRHIVGFIRGSLKQVTLLISNVLALYTQLHSLPFQPP----RNETMEKTKS- 220
           CL+GFEGT+  I   + G L +V   + N+L   T +HS P +P     + +TM K  S 
Sbjct: 137 CLDGFEGTNGIIKKIVAGGLSKVGTTVRNLL---TMVHSPPSKPKPKPIKAQTMTKAHSG 196

Query: 221 -SEFPDWMMDSEHKIVKSHPRNVHVDAIVALDGSGDFRSITEAVNEAPSYSNRRYIIYVK 280
            S+FP W+   + K++++    V  DA+VA DG+G+F +I++AV  AP YS +RY+I+VK
Sbjct: 197 FSKFPSWVKPGDRKLLQTDNITV-ADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVK 256

Query: 281 KGVYKENIDMKRKKTNIMFIGDGIGETIVTGSRNFLQGWTTFRTATVAVSGKGFIARDMT 340
           +GVY EN+++K+KK NIM +GDGI  T++TG+R+F+ GWTTFR+AT AVSG+GFIARD+T
Sbjct: 257 RGVYVENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDIT 316

Query: 341 FRNTAGPENHQAVALRVDSDQSAFFRCSFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGN 400
           F+NTAGPE HQAVA+R D+D   F+RC+  G+QDTLY HS+RQF+REC I GT+D+IFG+
Sbjct: 317 FQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGD 376

Query: 401 GAAVFQKCNIYTRVPLPLQKVTITAQGRKSPHQSTGFSIQDSFIYA---------TQPTY 460
             AVFQ C I  +  LP QK +ITAQGRK P++ TGF+IQ S I A         T  TY
Sbjct: 377 ATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATY 436

Query: 461 LGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFALGTLWYGEYKNYGPGASLSGRVKWP 520
           LGRPWKLYSRTVF+  YMS  + P GWLEW GNFAL TL+YGEY N GPGASL  RVKWP
Sbjct: 437 LGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWP 496

Query: 521 GYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLGL 542
           GYH +     A  FT    I G  WLP TGI F  GL
Sbjct: 497 GYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

BLAST of CsaV3_1G038620 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 478.4 bits (1230), Expect = 7.6e-135
Identity = 242/495 (48.89%), Postives = 325/495 (65.66%), Query Frame = 0

Query: 71  PNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSVAELAWSLAEM 130
           P S   ++I   +   R+    +++F+ FS   R + A+ DC +LLDFS  EL WS +  
Sbjct: 31  PASEFVSSINTIVVVIRQVSSILSQFADFSGDRRLQNAVSDCLDLLDFSSEELTWSASAS 90

Query: 131 KRIRAGKNEAPDEG-NLKAWLSAALSNQDTCLEGFEGTDRHIVGFIRGSLKQVTLLISNV 190
           +  +   N   D G + + WLSAALSNQ TC+EGF+GT   +   + GSL Q+  ++  +
Sbjct: 91  ENPKGKGNGTGDVGSDTRTWLSAALSNQATCMEGFDGTSGLVKSLVAGSLDQLYSMLREL 150

Query: 191 LALYT------------QLHSLPFQPP--RNETMEKTKSSEFPDWMMDSEHKIVKSHPRN 250
           L L               +   P  PP  +    ++ +S +FPDW+   + K+++S+ R 
Sbjct: 151 LPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLRDTDEDESLQFPDWVRPDDRKLLESNGRT 210

Query: 251 VHVDAIVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKENIDMKRKKTNIMFIGD 310
              D  VALDG+G+F  I +A+ +AP YS+ R++IY+KKG+Y EN+++K+KK NI+ +GD
Sbjct: 211 --YDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGD 270

Query: 311 GIGETIVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDSDQS 370
           GI  T+++G+R+F+ GWTTFR+AT AVSG+GF+ARD+TF+NTAGPE HQAVALR DSD S
Sbjct: 271 GIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLS 330

Query: 371 AFFRCSFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQKVT 430
            FFRC+  G+QDTLY H++RQFYREC I GT+D+IFG+G  VFQ C I  +  LP QK T
Sbjct: 331 VFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNT 390

Query: 431 ITAQGRKSPHQSTGFSIQDSFIYA---------TQPTYLGRPWKLYSRTVFLNTYMSGLV 490
           ITAQGRK  +Q +GFSIQ S I A         T  TYLGRPWKLYSRTVF+   MS +V
Sbjct: 391 ITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVV 450

Query: 491 QPRGWLEWYGNFALGTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHFIDG 542
           +P GWLEW  +FAL TL+YGE+ NYGPG+ LS RVKWPGYH   +   A  FT   FI G
Sbjct: 451 RPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKG 510

BLAST of CsaV3_1G038620 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 425.6 bits (1093), Expect = 5.9e-119
Identity = 222/518 (42.86%), Postives = 318/518 (61.39%), Query Frame = 0

Query: 42  ISQICADIEDRNSCISNIQLEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSF 101
           IS+ C+     N CI  + L+   S+ ++ N ++  +  AT+ +  +A+ + +  +    
Sbjct: 75  ISRTCSKSLYPNLCIDTL-LDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQM 134

Query: 102 SYREEMAIEDCKELLDFSVAELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCL 161
             R   A + C ELLD SV  L  +L+ +  +   ++ +    ++  WLS+A++N DTC 
Sbjct: 135 PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS----DVMTWLSSAMTNHDTCT 194

Query: 162 EGF---EGTDRHIVGFIRGSLKQVTLLISNVLALYT----QLHSLPFQPPRNETMEKTKS 221
           +GF   EG    +   + G++K ++ ++SN LA++      L  +P    R + +   ++
Sbjct: 195 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNR-KLLGTEET 254

Query: 222 SEFPDWMMDSEHKIVKSHPRNVHVDAIVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKK 281
            E P+W+   + +++ +    +  D  V+ DGSG F++I EA+ +AP +S+RR++IYVK 
Sbjct: 255 EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKA 314

Query: 282 GVY-KENIDMKRKKTNIMFIGDGIGETIVTGSRNFLQGWTTFRTATVAVSGKGFIARDMT 341
           G Y +EN+ + RKKTN+MFIGDG G+T++TG ++     TTF TAT A +G GFI RDMT
Sbjct: 315 GRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMT 374

Query: 342 FRNTAGPENHQAVALRVDSDQSAFFRCSFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGN 401
           F N AGP  HQAVALRV  D +  +RC+  G+QD LYVHS RQF+REC IYGT+D+IFGN
Sbjct: 375 FENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGN 434

Query: 402 GAAVFQKCNIYTRVPLPLQKVTITAQGRKSPHQSTGFSIQDSFIYAT---------QPTY 461
            A + Q CNIY R P+  QK+TITAQ RK P+Q+TG SI    + AT          PTY
Sbjct: 435 AAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTY 494

Query: 462 LGRPWKLYSRTVFLNTYMSGLVQPRGWLEWYGNFALGTLWYGEYKNYGPGASLSGRVKWP 521
           LGRPWKLYSR V++ + M   + PRGWLEW G FAL +L+YGEY N G G+ +  RVKWP
Sbjct: 495 LGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWP 554

Query: 522 GYHNIQDPTMARFFTSEHFIDGRTWLPKTGIKFTLGLS 543
           GYH I     A  FT   FI G +WLP TG+ F  GLS
Sbjct: 555 GYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of CsaV3_1G038620 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 417.5 bits (1072), Expect = 1.6e-116
Identity = 233/559 (41.68%), Postives = 327/559 (58.50%), Query Frame = 0

Query: 1   MSRVSLAIHIFMLIFLPVLQVSCFDVNVIYPDEQNNVTQSMISQICADIEDRNSCISNIQ 60
           +S + LAI + +   +     S   +N   P      +Q+ IS+ C        C+ ++ 
Sbjct: 47  VSSIVLAISLILAAAIFAGVRSRLKLNQSVPGLARKPSQA-ISKACELTRFPELCVDSLM 106

Query: 61  LEMGRSVNSNPNSVLSAAIRATINEARRAIESITKFSTFSFSYREEMAIEDCKELLDFSV 120
              G    S+   ++   +  T++    A+ S    S      R   A + C ELLD SV
Sbjct: 107 DFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSV 166

Query: 121 AELAWSLAEMKRIRAGKNEAPDEGNLKAWLSAALSNQDTCLEGFEGT-DRHIVGFIRGSL 180
             L+ +L+ +       +  P +  +  WLSAAL+N DTC EGF+G  D  +   +  +L
Sbjct: 167 DALSRALSSV----VSSSAKPQD--VTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAAL 226

Query: 181 KQVTLLISNVLALYTQLH------SLPFQPPRNETMEKTKSSEFPDWMMDSEHKIVKSHP 240
           + ++ L+SN LA+++  H       +P Q  R   +E+ +  +FP WM   E +I++   
Sbjct: 227 QNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEE-REEKFPRWMRPKEREILEMPV 286

Query: 241 RNVHVDAIVALDGSGDFRSITEAVNEAPSYSNRRYIIYVKKGVYKE-NIDMKRKKTNIMF 300
             +  D IV+ DG+G  ++I+EA+ +AP  S RR IIYVK G Y+E N+ + RKK N+MF
Sbjct: 287 SQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMF 346

Query: 301 IGDGIGETIVTGSRNFLQGWTTFRTATVAVSGKGFIARDMTFRNTAGPENHQAVALRVDS 360
           +GDG G+T+++G ++     TTF TA+ A +G GFIARD+TF N AGP  HQAVALR+ +
Sbjct: 347 VGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGA 406

Query: 361 DQSAFFRCSFEGHQDTLYVHSLRQFYRECNIYGTIDYIFGNGAAVFQKCNIYTRVPLPLQ 420
           D +  +RC+  G+QDTLYVHS RQF+REC+IYGT+D+IFGN A V Q C+IY R P+  Q
Sbjct: 407 DHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQ 466

Query: 421 KVTITAQGRKSPHQSTGFSIQDSFIYA---------TQPTYLGRPWKLYSRTVFLNTYMS 480
           K TITAQ RK P+Q+TG SI  S + A         +  TYLGRPWKL+SRTV++ +Y+ 
Sbjct: 467 KNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIG 526

Query: 481 GLVQPRGWLEWYGNFALGTLWYGEYKNYGPGASLSGRVKWPGYHNIQDPTMARFFTSEHF 540
           G V  RGWLEW   FAL TL+YGEY N GPG+ L  RV WPGY  I     A  FT   F
Sbjct: 527 GHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEF 586

Query: 541 IDGRTWLPKTGIKFTLGLS 543
           I G +WLP TG+ F  GLS
Sbjct: 587 IYGSSWLPSTGVSFLAGLS 597

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004135600.10.0e+00100.00putative pectinesterase/pectinesterase inhibitor 22 isoform X1 [Cucumis sativus]... [more]
XP_008450600.10.0e+0098.71PREDICTED: putative pectinesterase/pectinesterase inhibitor 22 isoform X1 [Cucum... [more]
XP_038879289.16.8e-30394.66putative pectinesterase/pectinesterase inhibitor 22 [Benincasa hispida][more]
KAG6590455.14.4e-29491.71putative pectinesterase/pectinesterase inhibitor 22, partial [Cucurbita argyrosp... [more]
XP_022961600.19.9e-29491.53putative pectinesterase/pectinesterase inhibitor 22 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M9W71.5e-19160.77Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3... [more]
Q9LXK71.8e-13648.94Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3... [more]
Q430622.0e-13550.53Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=... [more]
Q9SMY71.1e-13348.89Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3... [more]
Q9FK058.3e-11842.86Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A0A0LWC90.0e+00100.00Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569210 PE=3 SV=1[more]
A0A5D3CJ630.0e+0098.71Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004190... [more]
A0A1S3BNY20.0e+0098.71Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492140 PE=3 SV=1[more]
A0A6J1HCP74.8e-29491.53Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111462129 PE=3 SV=1[more]
A0A6J1HXZ07.6e-29291.16Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467863 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G05620.11.0e-19260.77Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G43270.11.3e-13748.94Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33220.17.6e-13548.89pectin methylesterase 44 [more]
AT5G53370.15.9e-11942.86pectin methylesterase PCR fragment F [more]
AT3G49220.11.6e-11641.68Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 37..192
e-value: 1.5E-30
score: 117.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 41..192
e-value: 1.7E-23
score: 83.7
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 6..197
e-value: 2.7E-22
score: 76.9
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 239..526
e-value: 6.3E-138
score: 458.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 38..197
e-value: 2.1E-28
score: 101.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 40..197
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 234..541
e-value: 1.6E-117
score: 394.5
NoneNo IPR availablePANTHERPTHR31707:SF205PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATEDcoord: 10..540
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 10..540
NoneNo IPR availableCDDcd15799PMEI-like_4coord: 69..196
e-value: 1.41235E-32
score: 120.207
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 264..283
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 383..392
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 238..540

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G038620.1CsaV3_1G038620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity