CsaV3_1G037490 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_1G037490 vs. NCBI nr
Match: XP_011659211.2 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653319.1 hypothetical protein Csa_023346 [Cucumis sativus])

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 544/544 (100.00%), Postives = 544/544 (100.00%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS
Sbjct: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA
Sbjct: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420
           DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM
Sbjct: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420

Query: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480
           NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY
Sbjct: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLSS 540
           VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLSS
Sbjct: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLSS 540

Query: 541 DHMV 545
           DHMV
Sbjct: 541 DHMV 544

BLAST of CsaV3_1G037490 vs. NCBI nr
Match: XP_008445721.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1026.2 bits (2652), Expect = 1.1e-295
Identity = 500/542 (92.25%), Postives = 522/542 (96.31%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           M  SPL+PLSLVF+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FS+L NFPTRNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH+FIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEE+LFLAI WNGGKVSA
Sbjct: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373

Query: 361 DQPLEALLNRTPITHG-SFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HG SFKLKSDYVKEPMTK AI+GIWKRLESQDIEGVTLA+IPYGGR
Sbjct: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M++ISES IPF HRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYMTPFVSKSPR A
Sbjct: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVK+ VDPYNFFWHEQSIPLS
Sbjct: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553

Query: 541 SD 542
           S+
Sbjct: 554 SN 555

BLAST of CsaV3_1G037490 vs. NCBI nr
Match: KAA0047096.1 (tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05062.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 963.8 bits (2490), Expect = 6.4e-277
Identity = 477/542 (88.01%), Postives = 499/542 (92.07%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           M  SPL+PLSLVF+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 1   MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FS+L NFPTRNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH+FIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEE+LFLAI WNGGKVSA
Sbjct: 241 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEALLNRTPITHG-SFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HG SFKLKSDYVKEPMTK AI+GIWKRLESQDIEGVTLA+IPYGGR
Sbjct: 361 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 420

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M++ISES IPF HRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYMTPFVSKSPR A
Sbjct: 421 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 480

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNK                       LVKVK+ VDPYNFFWHEQSIPLS
Sbjct: 481 YVNYRDLDIGSNNK-----------------------LVKVKTKVDPYNFFWHEQSIPLS 519

Query: 541 SD 542
           S+
Sbjct: 541 SN 519

BLAST of CsaV3_1G037490 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 916.0 bits (2366), Expect = 1.5e-262
Identity = 443/545 (81.28%), Postives = 499/545 (91.56%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSS-INHEEFIQCLLHHSPDTYLITKLVHTPINS 60
           MKFSPLI LSL F++VCSSSSL  A SSS I HEEF+QCLLHHSP+TY ITKLV+ PINS
Sbjct: 1   MKFSPLILLSLAFIIVCSSSSLLSATSSSYIKHEEFLQCLLHHSPNTYSITKLVYAPINS 60

Query: 61  SFSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120
           S+SSLLNFP+RNSRFSTPN P PLLIITP+NISHIQAA++CSKSHGLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFPSRNSRFSTPNIPNPLLIITPTNISHIQAAIICSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPT 180
           LSYVAY  FIVVDLINL+SVTVD E+S+AWV+SGATLGELYY+I +KS+TL FPAG+CPT
Sbjct: 121 LSYVAYVPFIVVDLINLRSVTVDAEKSSAWVQSGATLGELYYRIAEKSQTLAFPAGVCPT 180

Query: 181 VGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGGYG LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGG+F
Sbjct: 181 VGVGGHFSGGGYGILLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGNF 240

Query: 241 GIVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVS 300
           G+VVAWKVKLVPVP TVTIC+AKR+LEE AI+LIDQWQYV NKLEE+LFL+IF NGGK S
Sbjct: 241 GVVVAWKVKLVPVPETVTICAAKRTLEEGAIELIDQWQYVGNKLEEDLFLSIFLNGGKNS 300

Query: 301 AQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQI 360
           AQ GGK+NP ALFF+LFLG  +EL+AIL K FPELGLTKE+CKEKSWI SA SAANRFQI
Sbjct: 301 AQGGGKVNPTALFFSLFLGKADELVAILKKKFPELGLTKEDCKEKSWIGSAVSAANRFQI 360

Query: 361 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           EDQPLEALLNRTP+ +GS K+KSDYVKE +TK  I+GIW+RL+SQDIEGVTLA+IPYGGR
Sbjct: 361 EDQPLEALLNRTPLANGSLKIKSDYVKELITKVGIEGIWERLKSQDIEGVTLALIPYGGR 420

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M QIS+S+IPF HRAGI YQIGYI+GW+E+G++AEKRH+NWIREIYSYMTPFVSKSPRAA
Sbjct: 421 MGQISDSEIPFSHRAGIRYQIGYIVGWKEQGLDAEKRHINWIREIYSYMTPFVSKSPRAA 480

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRD DIGSNNKYGK++YK+A V+G KYFGNNFNRLV++K+ VDP NFF HEQSIPLS
Sbjct: 481 YVNYRDHDIGSNNKYGKVSYKRARVWGLKYFGNNFNRLVRLKTKVDPSNFFRHEQSIPLS 540

Query: 541 SDHMV 545
            +H +
Sbjct: 541 PNHRI 545

BLAST of CsaV3_1G037490 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 902.9 bits (2332), Expect = 1.3e-258
Identity = 440/543 (81.03%), Postives = 486/543 (89.50%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSS-INHEEFIQCLLHHSPDTYLITKLVHTPINS 60
           MKFSPLIPLSL F+++CSSSSL  A SSS I +EEF+QCLLHHSP+TY IT LV+ P NS
Sbjct: 1   MKFSPLIPLSLAFIILCSSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNS 60

Query: 61  SFSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120
           S+SSLLNF  RN RFSTP  PKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPT 180
           LSYVAYH FIVVDLINL SV VDVEQSTAWV+SGATLGELYY+I +KSRTL FPAG CPT
Sbjct: 121 LSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPT 180

Query: 181 VGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGGYG+LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVS 300
           GIVVAWKVKLVPVPATVTIC+A R+ +E AIKLIDQWQYVANKL+E+LFL+I   GGK+S
Sbjct: 241 GIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKIS 300

Query: 301 AQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQI 360
           AQ GGKINP ALFFTLFLG  +EL+ IL   FPEL L KE+CKE SWIESA   AN  QI
Sbjct: 301 AQ-GGKINPTALFFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQI 360

Query: 361 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           +DQPLEA LNRTP+ +GSFK+KSDYVKEP+TKAAI+GIW+RL+SQDIEGV L +IPYGGR
Sbjct: 361 DDQPLEAFLNRTPLANGSFKIKSDYVKEPITKAAIEGIWQRLKSQDIEGVNLGLIPYGGR 420

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M+QISES+IPFPHR G LY+IGYI+GWEE+GV+AEKRHLNWIREIYSYMTPFVSKSPRAA
Sbjct: 421 MSQISESEIPFPHRIGNLYKIGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSKSPRAA 480

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSN+KYGK +YK+AC++G KYFGNNFNRLV VK++VDPY+FF HEQSIP  
Sbjct: 481 YVNYRDLDIGSNDKYGKTSYKRACIWGKKYFGNNFNRLVHVKNEVDPYDFFRHEQSIPSF 540

Query: 541 SDH 543
             H
Sbjct: 541 PHH 542

BLAST of CsaV3_1G037490 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 6.3e-150
Identity = 265/543 (48.80%), Postives = 364/543 (67.03%), Query Frame = 0

Query: 17  CSSSSLW-------------IAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSSFSS 76
           CS+ S W             I  S +   E F++C   H P+     KLV+T  +  + S
Sbjct: 3   CSAFSFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMS 62

Query: 77  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 136
           +LN   +N RF +  TPKPL+I+TPSN SHIQA ++CSK  GLQIR RSGGHD EG+SY+
Sbjct: 63  ILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYI 122

Query: 137 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           +   F+VVDL N+ S+ +DV   TAWVE+GATLGE+YY I +K+  L FP G CPTVGVG
Sbjct: 123 SQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHFSGGGYG+L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ 
Sbjct: 183 GHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIA 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQE 316
           AWK+KLV VP+  TI S K+++E    +KL ++WQ +A K +++L L   +    ++   
Sbjct: 243 AWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNH 302

Query: 317 G-GKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G  K      F ++F G V+ L+ ++NK+FPELG+ K +CKE SWI++    S    F  
Sbjct: 303 GKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 436
            +   E LL+R+     +F +K DYVK+P+ + A+  I ++L  +D+      + PYGG 
Sbjct: 363 ANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGI 422

Query: 437 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 496
           M +ISES IPFPHRAGI+Y++ Y   WE++  E  ++H+NW+R +Y++ TP+VS++PR A
Sbjct: 423 MEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 543
           Y+NYRDLD+G  N     NY QA ++G KYFG NFNRLVKVK+ VDP NFF +EQSIP  
Sbjct: 483 YLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 542

BLAST of CsaV3_1G037490 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 9.1e-149
Identity = 257/509 (50.49%), Postives = 356/509 (69.94%), Query Frame = 0

Query: 33  EEFIQCLLHHSPDTYLITKLVHTPINSSFSSLLNFPTRNSRFSTPNTPKPLLIITPSNIS 92
           E F++CL  + P      KLV+T  +  + S+LN   +N RF++  TPKPL+I TP N+S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 93  HIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIVVDLINLKSVTVDVEQSTAWVES 152
           HIQ  ++CSK  GLQIR RSGGHD EG+SY++   F++VDL N+ SV +DV   TAWVES
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 153 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 212
           GATLGE+YY I + +  L FPAG CPTVG GGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLE-EDAIK 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S K+++E  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIDQWQYVANKLEEELFLAIFWNGGKVSAQEGGKINPIALFF-TLFLGNVNELMAILNKT 332
           L+++WQ +A   E+EL L   +    ++  +G     I  +F ++F G V+ L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEP 392
           FPELG+ K +CK+ SWI++    S    +   +   E LL+R+     +F +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 MTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPFPHRAGILYQIGYILGWEE 452
           + + A+  I ++L  +D+        PYGG M++ISES IPFPHRAGI Y+I YI  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEK 451

Query: 453 KGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKINYKQACVFGSK 512
           +  E  ++H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     NY QA ++G K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVKVKSDVDPYNFFWHEQSIP 538
           YFG NFNRLVKVK+ VDP NFF +EQSIP
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of CsaV3_1G037490 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.2e-148
Identity = 262/538 (48.70%), Postives = 371/538 (68.96%), Query Frame = 0

Query: 17  CSSSSLW-------------IAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSSFSS 76
           CS+ S W             I  S +   E F++C   + P+     KLV+T  N  + S
Sbjct: 3   CSTFSFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMS 62

Query: 77  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 136
           +LN    N RF++  TPKPL+I+TPS++SHIQ  ++CSK  GLQIR RSGGHD EG+SY+
Sbjct: 63  VLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYI 122

Query: 137 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           +   F++VDL N++S+ +DV   TAWVE+GATLGE+YY + +K+  L   AG CPTV  G
Sbjct: 123 SQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHF GGGYG L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWA+RGGG  SFGI+V
Sbjct: 183 GHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIV 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQE 316
           AWK++LV VP + T+ S K+ +E  + +KL+++WQ +A K +++L L   +    ++  +
Sbjct: 243 AWKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQ 302

Query: 317 GGKINPIALFF-TLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G     I  +F ++FLG V+ L+ ++NK+FPELG+ K +C++ SWI++    S    +  
Sbjct: 303 GKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 436
           ++   E LL+R+   +G+FK+K DYVK+P+ ++    I ++L  +DI     A+ PYGG 
Sbjct: 363 DNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGI 422

Query: 437 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 496
           M++ISES IPFPHRAGILY++ YI  WE++  E  ++HLNWIR IY++MTP+VSK+PR A
Sbjct: 423 MDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIP 538
           Y+NYRDLDIG N+     NY QA ++G KYFG NF+RLVKVK+ VDP NFF +EQSIP
Sbjct: 483 YLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIP 537

BLAST of CsaV3_1G037490 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.6e-148
Identity = 256/509 (50.29%), Postives = 356/509 (69.94%), Query Frame = 0

Query: 33  EEFIQCLLHHSPDTYLITKLVHTPINSSFSSLLNFPTRNSRFSTPNTPKPLLIITPSNIS 92
           E F++C   + P      KLV+T  +  + S+LN   +N RF++  TPKPL+IITP N+S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 93  HIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIVVDLINLKSVTVDVEQSTAWVES 152
           HIQ  ++CSK  GLQIR RSGGHD EG+SY++   F++VDL N+ SV +DV   TAWVE+
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 153 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 212
           GATLGE+YY I + +  L FPAG CPTVG GGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLE-EDAIK 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S K+++E  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIDQWQYVANKLEEELFLAIFWNGGKVSAQEGGKINPIALFF-TLFLGNVNELMAILNKT 332
           L+++WQ +A   E+EL L   +    ++  +G     I  +F ++F G V+ L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEP 392
           FPELG+ K +CK+ SWI++    S    +       E LL+R+     +F +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 MTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPFPHRAGILYQIGYILGWEE 452
           + + A+  I ++L  +D+        PYGG M++ISES IPFPHRAGI+Y+I YI  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

Query: 453 KGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKINYKQACVFGSK 512
           +  E  ++H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     NY QA ++G K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVKVKSDVDPYNFFWHEQSIP 538
           YFG NFNRLVKVK+ VDP NFF +EQSIP
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of CsaV3_1G037490 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.4e-146
Identity = 257/543 (47.33%), Postives = 363/543 (66.85%), Query Frame = 0

Query: 17  CSSSSLW-------------IAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSSFSS 76
           CS+ S W             I  S +   E F++C   + P+     K ++T  +  + S
Sbjct: 3   CSAFSFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMS 62

Query: 77  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 136
           +LN   +N RF++  TPKPL+I+TPSN+SHIQA+++CSK  GLQIR RSGGHD EGLSY+
Sbjct: 63  VLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYI 122

Query: 137 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           +   F +VDL N+ +V VD+   TAWVE+GATLGE+YY I + +    FP G CPTVGVG
Sbjct: 123 SQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHFSGGGYG+L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ 
Sbjct: 183 GHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIA 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQE 316
           AWK+KLV VP+  TI S K+++E    +KL ++WQ +A K +++L L   +    ++   
Sbjct: 243 AWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNH 302

Query: 317 G-GKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G  K      F ++FLG V+ L+ ++NK+FPELG+ K +CKE SWI++    S    +  
Sbjct: 303 GKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 436
            +   E LL+R+     +F +K DYVK+ + + A+  I ++L  +++      + PYGG 
Sbjct: 363 ANFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGI 422

Query: 437 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 496
           M++ISES IPFPHRAGI+Y++ Y   WE++  E  ++H+NW+R +Y++ TP+VS++PR A
Sbjct: 423 MDEISESAIPFPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 543
           Y+NYRDLD+G  N     NY QA ++G KYFG NFNRLVKVK+  DP NFF +EQSIP  
Sbjct: 483 YLNYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 542

BLAST of CsaV3_1G037490 vs. ExPASy TrEMBL
Match: A0A1S3BDD9 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 5.1e-296
Identity = 500/542 (92.25%), Postives = 522/542 (96.31%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           M  SPL+PLSLVF+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FS+L NFPTRNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH+FIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEE+LFLAI WNGGKVSA
Sbjct: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373

Query: 361 DQPLEALLNRTPITHG-SFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HG SFKLKSDYVKEPMTK AI+GIWKRLESQDIEGVTLA+IPYGGR
Sbjct: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M++ISES IPF HRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYMTPFVSKSPR A
Sbjct: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVK+ VDPYNFFWHEQSIPLS
Sbjct: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553

Query: 541 SD 542
           S+
Sbjct: 554 SN 555

BLAST of CsaV3_1G037490 vs. ExPASy TrEMBL
Match: A0A5D3C1K3 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00270 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.1e-277
Identity = 477/542 (88.01%), Postives = 499/542 (92.07%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           M  SPL+PLSLVF+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 1   MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FS+L NFPTRNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH+FIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEE+LFLAI WNGGKVSA
Sbjct: 241 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEALLNRTPITHG-SFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HG SFKLKSDYVKEPMTK AI+GIWKRLESQDIEGVTLA+IPYGGR
Sbjct: 361 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 420

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M++ISES IPF HRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYMTPFVSKSPR A
Sbjct: 421 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 480

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNK                       LVKVK+ VDPYNFFWHEQSIPLS
Sbjct: 481 YVNYRDLDIGSNNK-----------------------LVKVKTKVDPYNFFWHEQSIPLS 519

Query: 541 SD 542
           S+
Sbjct: 541 SN 519

BLAST of CsaV3_1G037490 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.5e-252
Identity = 423/538 (78.62%), Postives = 483/538 (89.78%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           MKFS L+PLSL F+VVCSSSS+W +ASS +NHEEF+ CLLHHSPD+  ITKLV+TPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           +S +LNF  RN RFSTPNTPKPLLIITPSNISH+QAAV+CSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH F+VVDLINL SVT++VEQSTAWV+SGATLGELYY+I +KSRTL FPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           IVVAWKVKLVPVPATVTIC+  R+LEE+AIKLIDQWQYVANKL+E+LFL I   GGK+ A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q G KINPIALFF+LFLG  +ELMAILNKTFP+LGLTKEECKE SWIES     N  QIE
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420
           DQPLE LLNRTP+ +G+ K+KSDYVKEP++KA I+ +W+RL+SQDIEG  L  IPYGGRM
Sbjct: 361 DQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRM 420

Query: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480
           +QISES+IPF HRAG LY+IGY+ GW E G+ AEK HLNWIR+IYSYMTPFV+KSPRAAY
Sbjct: 421 SQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPL 539
           VNYRDLDIGSN+KYGK +YK+A V+GSKYFG+NF+RLV VK++VDPYNFF HEQSIP+
Sbjct: 481 VNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPV 537

BLAST of CsaV3_1G037490 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.5e-252
Identity = 423/538 (78.62%), Postives = 483/538 (89.78%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           MKFS L+PLSL F+VVCSSSS+W +ASS +NHEEF+ CLLHHSPD+  ITKLV+TPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           +S +LNF  RN RFSTPNTPKPLLIITPSNISH+QAAV+CSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH F+VVDLINL SVT++VEQSTAWV+SGATLGELYY+I +KSRTL FPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           IVVAWKVKLVPVPATVTIC+  R+LEE+AIKLIDQWQYVANKL+E+LFL I   GGK+ A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q G KINPIALFF+LFLG  +ELMAILNKTFP+LGLTKEECKE SWIES     N  QIE
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420
           DQPLE LLNRTP+ +G+ K+KSDYVKEP++KA I+ +W+RL+SQDIEG  L  IPYGGRM
Sbjct: 361 DQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRM 420

Query: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480
           +QISES+IPF HRAG LY+IGY+ GW E G+ AEK HLNWIR+IYSYMTPFV+KSPRAAY
Sbjct: 421 SQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPL 539
           VNYRDLDIGSN+KYGK +YK+A V+GSKYFG+NF+RLV VK++VDPYNFF HEQSIP+
Sbjct: 481 VNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPV 537

BLAST of CsaV3_1G037490 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.9e-227
Identity = 386/536 (72.01%), Postives = 456/536 (85.07%), Query Frame = 0

Query: 4   SPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPD--TYLITKLVHTPINSSF 63
           SPLI LSL F+ + SSSSLW +A  +  HE F+QCLL HS +   Y I+K++HTPINSS+
Sbjct: 5   SPLISLSLAFIFL-SSSSLWASAFPN-KHESFLQCLLDHSLEYSNYSISKVIHTPINSSY 64

Query: 64  SSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLS 123
           SS+L+F  RN RFST  TPKPLLI+TPS++SHIQAAV+CSKS+G QIR RSGGHDFEGLS
Sbjct: 65  SSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLS 124

Query: 124 YVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVG 183
           YVA+  FI+VDLINL+S+++D++ +TAWV+SGATLGELYY I +KSRTL FPAGICPTVG
Sbjct: 125 YVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVG 184

Query: 184 VGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 243
           VGGHFSGGGYG LLRKYG+AADNVIDAYLVDA+G+FH+RESMGEDLFWAIRGGGGGSFGI
Sbjct: 185 VGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGI 244

Query: 244 VVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQ 303
           VVAWKVKLVPVPATVT+CSA R+LEE A+K+  +WQYVANKL+E LFL I W GGK+++Q
Sbjct: 245 VVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQ 304

Query: 304 EGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIED 363
           EGGK NP+A FF+LFLG  +EL++ILN  FPEL L K++C E SWIES       F  E 
Sbjct: 305 EGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTE- 364

Query: 364 QPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMN 423
             LEALL+RTP+T+ S K+KSDYVKEPM++A +QGIWKRL+SQDIEG  +  +PYGGRM+
Sbjct: 365 LTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMS 424

Query: 424 QISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYV 483
           QISES+ PF HRAG LY+IGYI GWEE+G++AEKRHL WIRE+Y YMTPFVSKSPRAAY 
Sbjct: 425 QISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYA 484

Query: 484 NYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIP 538
           NYRDLDIGSNNK+GK +YKQA V+G KYFGNNFNRLV VK+ VDPY+FF HEQSIP
Sbjct: 485 NYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 537

BLAST of CsaV3_1G037490 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 515.8 bits (1327), Expect = 4.3e-146
Identity = 253/542 (46.68%), Postives = 373/542 (68.82%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIA-----ASSSINHEEFIQCLLHHSPDTYLITKLVHT 60
           MKF       L+F    ++S+L ++     +++  N   F+QCL     D+ +++K++HT
Sbjct: 1   MKFQSFFSSVLIFF---TTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHT 60

Query: 61  PINSSFSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGH 120
           P ++SFSS+L    +N RFS P+ PKP+LI+TP   S +Q+AV C++  G+ IR RSGGH
Sbjct: 61  PNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGH 120

Query: 121 DFEGLSYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAG 180
           D+EGLSYV +  F+++DL NL+S+TVDV+  + WV++GAT+GELYY+IGKK+RTL FPAG
Sbjct: 121 DYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAG 180

Query: 181 ICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGG 240
           +CPTVGVGGHFSGGGYG+LLRK+G+AAD+VIDA +VDA G   +R  MGED FWAIRGGG
Sbjct: 181 VCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGG 240

Query: 241 GGSFGIVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNG 300
           G SF +V++WK+ L+ VP+TVT+ +  +  E+ A+K+I +WQ+VA+K+ ++LF+ +    
Sbjct: 241 GSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQR 300

Query: 301 GKVSAQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAAN 360
            K   +        A F  L+LG+V  L+ ++NK FPELGL +++C E SWIES    A 
Sbjct: 301 YKNMVR--------ASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA- 360

Query: 361 RFQIEDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIP 420
             ++ ++P+  L  RT  +  +FK KSD+V+EPM K AI  +W+RL+  + E   L   P
Sbjct: 361 --ELGEEPINVLTKRTRASL-AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTP 420

Query: 421 YGGRMNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKS 480
           +GG+M++I++ + PFPHR G +Y+I Y+  W     + +++++ W+  +Y  M+ FV+KS
Sbjct: 421 FGGKMSEIADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKS 480

Query: 481 PRAAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQS 538
           PR AY+N RDLD+G      +  Y++   +G KYF NNF RLV+VK+ VDP +FF  EQS
Sbjct: 481 PRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQS 524

BLAST of CsaV3_1G037490 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 515.4 bits (1326), Expect = 5.6e-146
Identity = 257/542 (47.42%), Postives = 375/542 (69.19%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           M+FS  +   L+F +  S     + ++   NHE+F++CL +   D  +  K++HT  +SS
Sbjct: 1   MEFSSFLFTILLFSLNISP----LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           F S+L+   +N RFS   TPKP+ IITP   S +Q  + C++ HG+ +R RS GH +EGL
Sbjct: 61  FFSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGL 120

Query: 121 SYVAYHQ-FIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPT 180
           SY+AY++ F V+DL NL+S+++DV+  T WV++GAT GELYY+IGK +++L FPAGI PT
Sbjct: 121 SYIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPT 180

Query: 181 VGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240
           VGVGG FSGGGYG+LLRKYG+AADN+IDA +VDA+G   DR++MGED FWAIRGGGG SF
Sbjct: 181 VGVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSF 240

Query: 241 GIVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVS 300
           G++++WKVKLV VP+T+T+   +++ +++A+++I +WQY A+K+ ++LF+       + +
Sbjct: 241 GVILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTT 300

Query: 301 AQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQI 360
            +   K    ALF  L++G VN L+A++ + FPELGL KE C+E SWIES    A+    
Sbjct: 301 LERSNKNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD--FP 360

Query: 361 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           + + L  L NR   T  SFK K D+V+EP+ +AAIQ IW+RLE+ +     + + P+GG+
Sbjct: 361 KGESLGVLTNRER-TSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGK 420

Query: 421 MNQISESKIPFPHRAGILYQIGYILGW---EEKGVEAEKRHLNWIREIYSYMTPFVSKSP 480
           M++++E + PFPHR G LY+I Y+  W   E+K      ++L W+  +Y +MTP+VSKSP
Sbjct: 421 MSEMAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSP 480

Query: 481 RAAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSI 539
           R AYVN++D+D+G      K  Y++   +G KYF NNF RLV+VK+ VDP +FF  EQSI
Sbjct: 481 RGAYVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSI 528

BLAST of CsaV3_1G037490 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 2.0e-143
Identity = 256/542 (47.23%), Postives = 365/542 (67.34%), Query Frame = 0

Query: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPIN-- 60
           M  S  +PL  + V+  S  ++   +S +   ++FI C+  ++   + + K    P    
Sbjct: 1   MGISKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNV 60

Query: 61  SSFSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFE 120
           S FS +L    +N RF   + PKP  I +P + SH+QA+++CSK   + +R+RSGGHD+E
Sbjct: 61  SMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYE 120

Query: 121 GLSYVAY--HQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGI 180
           GLSYV+     FI++DL  ++ V ++++ ++AWV+SGAT+GELYY+I +KS+  GFPAG+
Sbjct: 121 GLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGL 180

Query: 181 CPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGG 240
           C ++G+GGH +GG YGS++RKYG+ ADNV+DA +VDANG+  DR +MGED FWAIRGG G
Sbjct: 181 CSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAG 240

Query: 241 GSFGIVVAWKVKLVPVPATVTICSAKRSLEED-AIKLIDQWQYVANKLEEELFLAIFWNG 300
           GSFGI++AWK+KLVPVP TVT+ +  ++L++D   K+I +WQ VA+KL EELF+ + +N 
Sbjct: 241 GSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFN- 300

Query: 301 GKVSAQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAAN 360
               A  GG       +  LFLG    LM ++ K+FPELGLT ++C E SW+ES A  + 
Sbjct: 301 ---VAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISG 360

Query: 361 RFQIEDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIP 420
            F         L  ++P    SFK KSD+VK P+ ++ +QGI+K+L  +DI    +   P
Sbjct: 361 -FPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIP--LMIWNP 420

Query: 421 YGGRMNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKS 480
           YGG M +I ES+IPFPHR G+L+++ Y+  W +   +   RH+NWIR++YSYMTP+VS +
Sbjct: 421 YGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSSN 480

Query: 481 PRAAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQS 538
           PR AYVNYRDLD+G N K  K   KQA V+G+ YF NNFNRL+ +K+ VDP NFF HEQS
Sbjct: 481 PREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQS 534

BLAST of CsaV3_1G037490 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 503.8 bits (1296), Expect = 1.7e-142
Identity = 250/539 (46.38%), Postives = 373/539 (69.20%), Query Frame = 0

Query: 6   LIPLSLVFVVVCSSSSLWIAASSS-INHEEFIQCLLHHSPDTYLITKLVHTPINSSFSSL 65
           L+ L + F+++  S S + + S+   NHE F++CL H   +    ++++HT  + S+ S+
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINED--DSRIIHTSKDPSYFSI 66

Query: 66  LNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVA 125
           LN   +N RF    TPKP+ IITP   + +Q+ + C++ HG+ IR RSGGHD+EGLSY+A
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 126 YHQ-FIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 185
             + F+V+DL NL+S+T+DV+  T WV+SGAT+GELYY+IGK S++L FPAG+ PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 186 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 245
           G F GGGYG+L+RKYG++ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 246 AWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQEG 305
           +WK++L+ VP+ VT+    ++ E++A+ +I++WQY+A+K+  +LF         + A   
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLF---------IRAMLQ 306

Query: 306 GKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQP 365
            +    A F  L+LG V++L+A++   FPELGL    C+E SWIES       + I+ + 
Sbjct: 307 KETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL-----WFIKGES 366

Query: 366 LEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQI 425
           +E L  R   T  SFK K D+++EP+ K AIQ +W+R E+ +     + + P+GG+M++I
Sbjct: 367 MEILAKRKR-TSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEI 426

Query: 426 SESKIPFPHRAGILYQIGYILGW---EEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 485
           ++++IPFPHR G LY+I Y+  W   E+K     +++L W+  +Y +MTP+VSKSPR AY
Sbjct: 427 ADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAY 486

Query: 486 VNYRDLDIGSNNKYG-KINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPL 539
           VN+RD+D+G       K  Y++A V+G KYF NNF+RLV+VK++VDP +FF  EQSIP+
Sbjct: 487 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPI 528

BLAST of CsaV3_1G037490 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 499.6 bits (1285), Expect = 3.2e-141
Identity = 244/518 (47.10%), Postives = 364/518 (70.27%), Query Frame = 0

Query: 24  IAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS--FSSLLNFPTRNSRFSTPNTPK 83
           +  SSS   ++F++CL+ +S  ++ IT    +P  ++  F   L    +N R+ TP+ PK
Sbjct: 22  VPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPK 81

Query: 84  PLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQ--FIVVDLINLKSV 143
           P+ I  P   +H+QAAVVC+K   L +R+RSGGHD+EGLS+VA  +  F++VDL  L+ V
Sbjct: 82  PVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQV 141

Query: 144 TVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYG 203
            VD++ ++AW  +GAT+GE+YY+I +KS+T GFPAG+C ++G+GGH  GG YGS++RK+G
Sbjct: 142 DVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFG 201

Query: 204 VAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTIC 263
           + ADNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+++AWK+KLVPVPATVT+ 
Sbjct: 202 LGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVF 261

Query: 264 SAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQEGGKINPIALFFTLFLGN 323
           +  ++LE+D  K++ +W+ +A+KL+++LF+ +  +    + + G +   ++ +   FLG+
Sbjct: 262 TVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMS-YQAQFLGD 321

Query: 324 VNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPITHGSFK 383
            N L+ ++ K+FPELGLTK++C E SWI+S    A  F     P EALL    +    FK
Sbjct: 322 SNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAG-FPNSAAP-EALLAGKSLFKNHFK 381

Query: 384 LKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPFPHRAGILYQ 443
            KSD+VKEP+    ++G+W+R   +D         PYGG M++ISES+IPFPHR G L++
Sbjct: 382 AKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRISESEIPFPHRNGTLFK 441

Query: 444 IGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKINY 503
           I ++  W++ G  +E+RH+ WIRE+YSYM  +VSK+PR AYVNYRDLD+G+N   G+ + 
Sbjct: 442 IQWLSTWQD-GKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE--GETDA 501

Query: 504 KQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIP 538
           ++   +G+KY+  NF RLVK+K + DP NFF HEQS+P
Sbjct: 502 RE---WGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528