CsaV3_1G028900 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_1G028900
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Descriptionomega-6 fatty acid desaturase, chloroplastic
Locationchr1: 15819193 .. 15823700 (+)
RNA-Seq ExpressionCsaV3_1G028900
SyntenyCsaV3_1G028900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAACTTTTGTTTTTGCTTACAATTTCGTTGTGGGGGCAGAAAAAAAATGAGTGATAACAACGCCTACTTGAACACTAGTGGCGCAAGCTCGAAAGGGCTAACCCCTTTCAGAGGATTCTATCCACATCTTAAAGAACGATTGGTGAAAGATTTATTCCCAATTTCCCATTTCAAATTGCAAACTTCCATACTTAAGGGGATACCCATTAAGAAATTTCCCTCTTTCCCCCCTCTGTTGCCTCTTATTCGAATAGCCCAAGCAAGCAAGCTAAGGGGGCCTGCTTAATTGGGATTTCAGTAATTCTTCACATAATGGCTTGCAGACTCGTAGATTCCATGGCTCTTTTCAATGTATGTTTTCTTCTTTTTAATCTCTCCCTGCTATTGTACTTGTGTTCTTCTTTTCTCTTATTATTTTGGTTTGATTATCAGGGCCCCCATCATAATTTTGCTCGAACCCAGAAAATTGCATCCCACTGTTCTCCAGGTATGTTTTAGTATCTTCCTTTTGAGAATTATTTGAAGCTGTTTAAGAATTTGTAATTACTTGATAAACTGAATCGTCTAGCGGTTGTCTTATTACAGTGTGACACAGTTAGAGTATATTCATTGAAGATTCAATCTCATTTCCTTTGTCACTCCGGAAGAAACCTTGCTGTTTCAGACATGGTTTCTTTTAATAAAATATTGAGTAAAATTGGAAATTAGATAGATTTAGAGTCTAGCTCGTTCTGGAAGTTTAGATTTTCAATCACATTGATAAATTTTCATATCCTCATTCAACTTAAGCATGGATTTTGATATGCCATAAAATGAATTAATTGACTAGGAACCAACATAGCTTTGATTTTCCATTATCAATTTAGACAGTTCAAACATGCTTATTCCGATAGACAGGTATATTGCAAGTGAAGGGGGAAATATTATTTCAGAAGAAAATCAAGCGACAAAACTATTTACTTCCTGTGAAGAGGGCTGCAATTCTACGAGCTATTGCTGTTCCAGCTGCGCCACCATCTTCTTCAGCAGATAGTGCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTTGAGCAAATTGGAGAACCTCTTCCTGATAATGTTAGGTTAAAAGATGTTGTTGAGTCCCTTCCAAAAGAGGTACTTTATTTTGTTGCAACATACTGGTGTTTAACATTTGTCCTTGAGGCAGTTTCATTTTCTTTTCTTCTGTCAGATAAAATTTTGATGATGCTATCTAGTTCATGCATACGATCGTGTTTCTTTTACTTATTATATCTTATTTTTTCATTTTTTTAAAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTACTCATTAGGCCTTGTCATGATTGCAAAAGCTCCATGGTATCTACTACCTTTGGCTTGGGCATGGACTGGAACAGCAATAACTGGGGTTAGTCTCAATATTCTTTCTGGTTGCTTCCCTTTCCCTTTCTCTTCTTCTCATCCTTATCAGACTTGCAACTCTAGCCACTGAAGTTTCTTTCCTCCTCAAGTTCTTTGTTATAGGTCATGATTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGACATTGTGGGTACTTTGGCTTTCATGCCTCTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGTATTAATAATCTTTCCTCTATCTCTCTCTCTCTTTGAAATACTTGTGTGAGCTCTATCTTCCTGCAGAAGCATTGTTCAGTAATATGTAAAGAAATGTCTTATTGAGGTTGGATAGCATCTAGTTAAGCAATGCAAAAGCCTTGATAAGTAATATGAGCAGAACAATACGGATGGATGTTAGTGTGTTATTGTAAGGGACCTAATGAGAGATATATGAGGGTTATTAGTAGAATATTAGTATGGTTATTAGAAGGGGCCAATTAGTAAATAGCTAGTAAGCTGGTTAGAGTTTTTAGTTATAAATTCAGGGAATGGGTTGAGAGAAAGGTGCGAAGAATTTTGTGGGATTTCCTTGTGAGGAATTTGGGAAAGTCTAGCCCTCTGGGGGTTACGTTTTTTACTTTTCTGTCTAATTAGTTCCCTTACACTTATAAATTTATAATCAGTACCTCGATAGTACTAGGTTTGTTCACATGACGTCTTATTTACTTGTCATGGTTAGTTCTCCTTGTATCTTGGGATCAAGTTAACAGAATTTCTCCATATGTTTTGCATTTTGCCTAGAGTCAAATATTGCTGTGTTAATTAAGTTTGAAATACTCCACATATGACCAAATAGGAATCCATATTTTATAAGTTTGGTTTGTTACTTGCATCTTAACGATTTATGCCTTTCATCGTCTAGTTTTAGCTAACCTTTCATTTTTATTCTTATCCACATATTATAGGCTGGAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCTCCTCTTTTGCGTAAAGCAATCATATATGGTTATGGTCCATTTCGACCGTGGATGTCTATAGCACATTGGTAAGTTTACCTTGGATGGTTACTTTGCTAGAATAGTTTGACTTTCTATGAAAATTTATTGTACGTGATTTGACCAAGTTGTCTGTTTCATTAATATGCACCAATTTGCTCTTATGCATGATAGGTTGTTGTGGCACTTTGATTTGAAGAAGTTCAGACCAAATGAAGTAAAAAGAGTGAAGATAAGCTTAGCTTGTGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGACTGGGATTCTTGGATGGGTCAAGTTCTGGTTAATGCCATGGCTGGGATACCACTTTTGGGTAACGTCATTTGCTTGCCTCTTCTTATTACAGTTTCTTCTATAGTCCTATGATCAATTCAATTGATGATCTGCGTTGGATAGTGATTTGTCATGGTTCTTCATGATGTGACAATGGATTAAGAAACTAGTTAACAATCAGAATTAGAGGATATATGGCAGGGTAGACTTTAATGAAAATGAACCATGTAGCTTAGCCGGTGGTATCCAAGTATATCTTGATATTGTTGAAACATCAATCTTAATGAAGCTTACATCTTGCACTAATATTCTTGAAGCAAATAAAAGAATGTGAATACCATGCTTTCGAGATCATGAGACAATGATACGATGTTTGTGCCACTTTTCTTCATTCATAGAGGGACGATATTTATTGTTCTTGTTCTGCATAACTTTGAAATTGTCCCAGATAAAATTTGTTGGTTTTGCTTTTGTAAACTTAACTTCTATTTAAATTACCTTTTGTTTCTTTCTTAGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCGTTTAAGTCATCAGAAGAGTGGAACGCAGCTCAAGCACAGCTCAATGGAACAGTTCACTGTGCTTATCCTCAATGGTACTGAATCATTCATTTCAGTGTCTTTTTTTCCTTTTCTTTTCTTTTCTCTTTTCTTGCTTAATTCGACTCTAAGCCGATAAAAAGGCACATCGGAAAAAGATTCAGGCACTGTTCATGAGATTCTCTAGTCTTGAAATTGCTAGATAATTCATGTTAACTAAGATTTATTACCATAAGTCACATTGACTTAGCTTCTAAGTTTATCCTCTCCCATCTACAGGATCGAGATTCTCTGCCATGACATCAATGTGCACATTCCCCACCATATTTCTCCAAGAATACCGAGTTACAACTTACGGGCAGCTCATAAGTCTCTTCAAGAAAACTGGGGAAAGGTAATGGGGTTAAACCAAATGCATATGTACATAGTTCGATGTTCAGATCTTGCAAAATCTAATGGTTGCCTTGTTTATTGCATATATTTTCCCCCATTGATGCTAATAATCTAGTTCTTTTCTTCCACTTTGTTACACATTCTGTAGCATCTGAATGAGGCTTCATGGAATTGGCGATTAATGAAGACGATCATGACGATATGCCATGTATACAACAAAGAGGAAAATTATGTCGCATTTGACCGACTCAATAAAGAAGATTCTTATCCCATTACATTCCTCAAGAAAGTCATGCCTGATCTTGCTTGATTTAATCTTTACCTCAACCTTCGGGAACACAGTCCACAGACACTTGCTGCTTTCTTTCTCTGTGCAATGTTTTCTCCACAGATTTTGAAGAACGAATGGAAGGAAAGCTTGGTTTAGTTGACTGCCATATATTGAGTTAAAGAATTTCTCTATTCCATTTTCCAGTCTCTCTCTCCTCAAATAGCAATATTATGTAACCTTTTCTTTTAAGGTAGTTGTGATCTAAAGAGGGAAAACAGACCTTTGGTGTTTTATGTTGCCAACTTCTTAAAAAGGTGGAATGGAATATGGAAACATTCTCTTTATCAAAGACACCTACTGACAAAGATCAATACAACACAACCATTATCATTGCAAGCGAAAATTTATTCTCTTTTACCATTCAAATTCTTTTATCTTCAAGAAACC

mRNA sequence

ATGGCTTGCAGACTCGTAGATTCCATGGCTCTTTTCAATGGCCCCCATCATAATTTTGCTCGAACCCAGAAAATTGCATCCCACTGTTCTCCAGGTATATTGCAAGTGAAGGGGGAAATATTATTTCAGAAGAAAATCAAGCGACAAAACTATTTACTTCCTGTGAAGAGGGCTGCAATTCTACGAGCTATTGCTGTTCCAGCTGCGCCACCATCTTCTTCAGCAGATAGTGCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTTGAGCAAATTGGAGAACCTCTTCCTGATAATGTTAGGTTAAAAGATGTTGTTGAGTCCCTTCCAAAAGAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTACTCATTAGGCCTTGTCATGATTGCAAAAGCTCCATGGTATCTACTACCTTTGGCTTGGGCATGGACTGGAACAGCAATAACTGGGTTCTTTGTTATAGGTCATGATTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGACATTGTGGGTACTTTGGCTTTCATGCCTCTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGCTGGAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCTCCTCTTTTGCGTAAAGCAATCATATATGGTTATGGTCCATTTCGACCGTGGATGTCTATAGCACATTGGTTGTTGTGGCACTTTGATTTGAAGAAGTTCAGACCAAATGAAGTAAAAAGAGTGAAGATAAGCTTAGCTTGTGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGACTGGGATTCTTGGATGGGTCAAGTTCTGGTTAATGCCATGGCTGGGATACCACTTTTGGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCGTTTAAGTCATCAGAAGAGTGGAACGCAGCTCAAGCACAGCTCAATGGAACAGTTCACTGTGCTTATCCTCAATGGATCGAGATTCTCTGCCATGACATCAATGTGCACATTCCCCACCATATTTCTCCAAGAATACCGAGTTACAACTTACGGGCAGCTCATAAGTCTCTTCAAGAAAACTGGGGAAAGCATCTGAATGAGGCTTCATGGAATTGGCGATTAATGAAGACGATCATGACGATATGCCATGTATACAACAAAGAGGAAAATTATGTCGCATTTGACCGACTCAATAAAGAAGATTCTTATCCCATTACATTCCTCAAGAAAGTCATGCCTGATCTTGCTTGA

Coding sequence (CDS)

ATGGCTTGCAGACTCGTAGATTCCATGGCTCTTTTCAATGGCCCCCATCATAATTTTGCTCGAACCCAGAAAATTGCATCCCACTGTTCTCCAGGTATATTGCAAGTGAAGGGGGAAATATTATTTCAGAAGAAAATCAAGCGACAAAACTATTTACTTCCTGTGAAGAGGGCTGCAATTCTACGAGCTATTGCTGTTCCAGCTGCGCCACCATCTTCTTCAGCAGATAGTGCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTTGAGCAAATTGGAGAACCTCTTCCTGATAATGTTAGGTTAAAAGATGTTGTTGAGTCCCTTCCAAAAGAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTACTCATTAGGCCTTGTCATGATTGCAAAAGCTCCATGGTATCTACTACCTTTGGCTTGGGCATGGACTGGAACAGCAATAACTGGGTTCTTTGTTATAGGTCATGATTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGACATTGTGGGTACTTTGGCTTTCATGCCTCTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGCTGGAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCTCCTCTTTTGCGTAAAGCAATCATATATGGTTATGGTCCATTTCGACCGTGGATGTCTATAGCACATTGGTTGTTGTGGCACTTTGATTTGAAGAAGTTCAGACCAAATGAAGTAAAAAGAGTGAAGATAAGCTTAGCTTGTGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGACTGGGATTCTTGGATGGGTCAAGTTCTGGTTAATGCCATGGCTGGGATACCACTTTTGGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCGTTTAAGTCATCAGAAGAGTGGAACGCAGCTCAAGCACAGCTCAATGGAACAGTTCACTGTGCTTATCCTCAATGGATCGAGATTCTCTGCCATGACATCAATGTGCACATTCCCCACCATATTTCTCCAAGAATACCGAGTTACAACTTACGGGCAGCTCATAAGTCTCTTCAAGAAAACTGGGGAAAGCATCTGAATGAGGCTTCATGGAATTGGCGATTAATGAAGACGATCATGACGATATGCCATGTATACAACAAAGAGGAAAATTATGTCGCATTTGACCGACTCAATAAAGAAGATTCTTATCCCATTACATTCCTCAAGAAAGTCATGCCTGATCTTGCTTGA

Protein sequence

MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAILRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDVKAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVAFDRLNKEDSYPITFLKKVMPDLA*
Homology
BLAST of CsaV3_1G028900 vs. NCBI nr
Match: XP_004146685.1 (omega-6 fatty acid desaturase, chloroplastic isoform X1 [Cucumis sativus] >KGN65155.1 hypothetical protein Csa_019883 [Cucumis sativus])

HSP 1 Score: 941.8 bits (2433), Expect = 2.1e-270
Identity = 443/443 (100.00%), Postives = 443/443 (100.00%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120
           LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV
Sbjct: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRLNKEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of CsaV3_1G028900 vs. NCBI nr
Match: XP_038880078.1 (omega-6 fatty acid desaturase, chloroplastic [Benincasa hispida])

HSP 1 Score: 925.6 bits (2391), Expect = 1.6e-265
Identity = 432/443 (97.52%), Postives = 437/443 (98.65%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRL DSM LFNGPH N ARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120
           LRA+AVP APPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKD+VESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASY+LGLVMIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVY+KEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRLNKEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of CsaV3_1G028900 vs. NCBI nr
Match: XP_008443861.1 (PREDICTED: omega-6 fatty acid desaturase, chloroplastic [Cucumis melo])

HSP 1 Score: 922.5 bits (2383), Expect = 1.3e-264
Identity = 431/443 (97.29%), Postives = 438/443 (98.87%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRLVDSMALFNGPH N ARTQKIASHCSPG LQVKGEILFQKKIKRQNYLLPVK+AAI
Sbjct: 1   MACRLVDSMALFNGPHQNLARTQKIASHCSPGTLQVKGEILFQKKIKRQNYLLPVKKAAI 60

Query: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120
           LRA+AVP APPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKD+VESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEFDSAP+LRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420
           NVHIPHH+SPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT+CHVY+KEENYVA
Sbjct: 361 NVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRLNKEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of CsaV3_1G028900 vs. NCBI nr
Match: XP_022930407.1 (omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 902.1 bits (2330), Expect = 1.9e-258
Identity = 417/443 (94.13%), Postives = 433/443 (97.74%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRL DS+ LF GPH N+ARTQKIASH +PGIL VKG+I+FQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120
           LRA+AVP APPSSSAD AEYRKQLSESYGFEQIGEPLP+NVRLKD++ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASY+LGL+MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDSAPLLRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVY+KEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRL  EDSYPITFLKKVMPDLA
Sbjct: 421 FDRLKPEDSYPITFLKKVMPDLA 443

BLAST of CsaV3_1G028900 vs. NCBI nr
Match: XP_023529482.1 (omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 901.0 bits (2327), Expect = 4.2e-258
Identity = 416/443 (93.91%), Postives = 433/443 (97.74%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRL DS+ LF GPH N+ARTQKIASH +PGIL VKG+I+FQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120
           LRA+AVP APPSSSAD AEYRKQLSESYGFEQIGEPLP+NVRLKD++ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASY+LGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDSAPLLRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420
           NVH+PHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVY+KEENYVA
Sbjct: 361 NVHVPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRL  EDSYPITFL+KVMPDLA
Sbjct: 421 FDRLKPEDSYPITFLRKVMPDLA 443

BLAST of CsaV3_1G028900 vs. ExPASy Swiss-Prot
Match: P48627 (Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 1.8e-208
Identity = 336/445 (75.51%), Postives = 384/445 (86.29%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIAS-HCSPGILQVKGEILFQKKIKRQNYLLPV-KRA 60
           MA R+ DS+  F GP     R  K+AS   SPG+  V+   L  K  +R  +L+P  KR 
Sbjct: 1   MASRIADSLFAFTGPQQCLPRAPKLASARLSPGVYAVRPIDLLLKGTRR-TFLVPAKKRI 60

Query: 61  AILRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEIN 120
             ++A+ VP APP  SAD+AE R+QL+ESYGF+QIG+ LPDNV LKD++++LPKEVFEI+
Sbjct: 61  GCIKAVFVPVAPP--SADNAEDREQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEID 120

Query: 121 DVKAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNK 180
           DVKAWKSVL+SV SY+LGL MIAKAPWYLLPLAWAWTGTA+TGFFVIGHDCAHKSFS+NK
Sbjct: 121 DVKAWKSVLISVTSYALGLFMIAKAPWYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSKNK 180

Query: 181 LVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAII 240
           LVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEFDS+P+LRKAII
Sbjct: 181 LVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFDSSPVLRKAII 240

Query: 241 YGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGW 300
           +GYGP RPW+SIAHW+ WHF+L+KFRP+EV RVKISLACVFAFM +GWPLIIYK G+LGW
Sbjct: 241 FGYGPIRPWLSIAHWVNWHFNLRKFRPSEVNRVKISLACVFAFMAVGWPLIIYKVGVLGW 300

Query: 301 VKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCH 360
           VKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEILCH
Sbjct: 301 VKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEILCH 360

Query: 361 DINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENY 420
           DINVHIPHHISPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY+KEENY
Sbjct: 361 DINVHIPHHISPRIPSYNLRAAHQSIQENWGKYTNLATWNWRLMKTIMTVCHVYDKEENY 420

Query: 421 VAFDRLNKEDSYPITFLKKVMPDLA 444
           + FDRL  E+S PITFLKK MPD A
Sbjct: 421 IPFDRLAPEESQPITFLKKAMPDYA 442

BLAST of CsaV3_1G028900 vs. ExPASy Swiss-Prot
Match: P46312 (Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD6 PE=1 SV=2)

HSP 1 Score: 708.8 bits (1828), Expect = 4.0e-203
Identity = 330/447 (73.83%), Postives = 382/447 (85.46%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIA---SHCSPGILQVKG-EILFQKKIKR-QNYLLPV 60
           MA R+ DS+  F GP     R  K+A   +  SPG+  VK  ++L + +  R +  + PV
Sbjct: 1   MASRIADSLFAFTGPQQCLPRVPKLAASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPV 60

Query: 61  K-RAAILRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEV 120
           K R   ++A+A P APP  SADSAE R+QL+ESYGF QIGE LP+NV LKD++++LPKEV
Sbjct: 61  KRRIGCIKAVAAPVAPP--SADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEV 120

Query: 121 FEINDVKAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 180
           FEI+D+KA KSVL+SV SY+LGL MIAK+PWYLLPLAWAWTGTAITGFFVIGHDCAHKSF
Sbjct: 121 FEIDDLKALKSVLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 180

Query: 181 SRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLR 240
           S+NKLVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S+P++R
Sbjct: 181 SKNKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMR 240

Query: 241 KAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTG 300
           KAII+GYGP RPW+SIAHW+ WHF+LKKFR +EV RVKISLACVFAFM +GWPLI+YK G
Sbjct: 241 KAIIFGYGPIRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVG 300

Query: 301 ILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIE 360
           ILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIE
Sbjct: 301 ILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIE 360

Query: 361 ILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNK 420
           ILCHDINVHIPHHISPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY+K
Sbjct: 361 ILCHDINVHIPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDK 420

Query: 421 EENYVAFDRLNKEDSYPITFLKKVMPD 442
           EENY+ FDRL  E+S PITFLKK MP+
Sbjct: 421 EENYIPFDRLAPEESQPITFLKKAMPN 445

BLAST of CsaV3_1G028900 vs. ExPASy Swiss-Prot
Match: P48628 (Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 6.8e-203
Identity = 322/423 (76.12%), Postives = 362/423 (85.58%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MAC L DS+ LF G +      + IA+  SPGI  +    L QK+ +RQ   +   +  +
Sbjct: 1   MACTLADSLLLFKGSYQKPVLRRDIAARYSPGIFSLNSNGLIQKRFRRQRNFVTRNKVTV 60

Query: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120
           + A+A+P  P  +  +SAEYRKQL+E YGF Q+GEPL D+V LKDV+  LPKEVFEI+DV
Sbjct: 61  IHAVAIPVQP--APVESAEYRKQLAEDYGFRQVGEPLSDDVTLKDVINPLPKEVFEIDDV 120

Query: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVL+SV SY+LGL MI+KAPWYLLPLAW WTGTAITGFFVIGHDCAH+SFS NKLV
Sbjct: 121 KAWKSVLISVTSYALGLFMISKAPWYLLPLAWVWTGTAITGFFVIGHDCAHRSFSSNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHD+HHAKTNML EDTAWHPVWK+EF+S PLLRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDRHHAKTNMLREDTAWHPVWKDEFESTPLLRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300
           YGPFR WMSIAHWL+WHFDLKKFRP+EV RVKISLACVFAF+ IGWPLIIYKTGI+GW+K
Sbjct: 241 YGPFRCWMSIAHWLMWHFDLKKFRPSEVPRVKISLACVFAFIAIGWPLIIYKTGIMGWIK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAP+IPFK SEEWN AQAQLNGTVHC YP+WIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPYIPFKYSEEWNRAQAQLNGTVHCDYPKWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWG++LNEASWNWRLMKTIMT+C VY+KE++   
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGQYLNEASWNWRLMKTIMTVCQVYDKEKSLCC 420

Query: 421 FDR 424
             R
Sbjct: 421 LRR 421

BLAST of CsaV3_1G028900 vs. ExPASy Swiss-Prot
Match: P48629 (Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD6 PE=1 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 7.1e-184
Identity = 290/390 (74.36%), Postives = 343/390 (87.95%), Query Frame = 0

Query: 56  KRAAILRAIAVPAAPPSS--SADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKE 115
           +++ +++A+AVP A PS+    D+ E+ KQL+E YGF+QIGEPLPD+V ++D++ SLPK+
Sbjct: 58  RKSTLVQAVAVPVAQPSAFPPTDNTEHLKQLAERYGFQQIGEPLPDDVTMRDIITSLPKQ 117

Query: 116 VFEINDVKAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKS 175
           VFEIND KAW +VL+SV SY+LG+ MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKS
Sbjct: 118 VFEINDTKAWGTVLISVTSYALGIFMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKS 177

Query: 176 FSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLL 235
           FS+NKLVEDIVGTLAFMPLIYPYEPWRFKHDQHH KTNML EDTAW P+ KE+ +S+P L
Sbjct: 178 FSKNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHTKTNMLREDTAWLPIMKEDIESSPGL 237

Query: 236 RKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKT 295
           RKA+IY YGP R WMSIAHWL  HF+LK FR +EVKR  ISLA VFAFM IGWPLIIYKT
Sbjct: 238 RKALIYAYGPLRTWMSIAHWLKVHFNLKDFRQSEVKRATISLAAVFAFMVIGWPLIIYKT 297

Query: 296 GILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWI 355
           GI+GW+KFWLMPWLGYHFWMSTFT+VHHTAPHIPFKSS+EWNAAQAQL+GTVHC YP+WI
Sbjct: 298 GIVGWIKFWLMPWLGYHFWMSTFTIVHHTAPHIPFKSSKEWNAAQAQLSGTVHCDYPRWI 357

Query: 356 EILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYN 415
           EILCHDI+VHIPHHISP+IPSYNLRAA++SL ENWG++LN+   NWRLM+TIMT CH+Y+
Sbjct: 358 EILCHDISVHIPHHISPKIPSYNLRAANQSLNENWGEYLNKPKSNWRLMRTIMTTCHIYD 417

Query: 416 KEENYVAFDRLNKEDSYPITFLKKVMPDLA 444
           K+ NYV+F++   E+S PI+  K+VMPD A
Sbjct: 418 KDGNYVSFEKAVPEESQPISIPKRVMPDYA 447

BLAST of CsaV3_1G028900 vs. ExPASy Swiss-Prot
Match: P20388 (Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=desA PE=1 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 8.3e-108
Identity = 176/332 (53.01%), Postives = 231/332 (69.58%), Query Frame = 0

Query: 96  PLPDNVRLKDVVESLPKEVFEINDVKAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWT 155
           P+ D ++L+D++++LPKE FE    KAW SVL+++ + ++G + I   PWY LP+ W WT
Sbjct: 20  PIAD-LKLQDIIKTLPKECFEKKASKAWASVLITLGAIAVGYLGIIYLPWYCLPITWIWT 79

Query: 156 GTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEE 215
           GTA+TG FV+GHDC H+SF++ + V D+VG +AF PLIYP+  WR  HD HH  TN +E 
Sbjct: 80  GTALTGAFVVGHDCGHRSFAKKRWVNDLVGHIAFAPLIYPFHSWRLLHDHHHLHTNKIEV 139

Query: 216 DTAWHPVWKEEFDSAPLLRKAIIYGY-GPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKIS 275
           D AW P   E F ++P + +       GPF    SI HW L HF L  F   +  +VK+S
Sbjct: 140 DNAWDPWSVEAFQASPAIVRLFYRAIRGPFWWTGSIFHWSLMHFKLSNFAQRDRNKVKLS 199

Query: 276 LACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEW 335
           +A VF F  I +P +I  TG+ G+VKFWLMPWL YHFWMSTFT+VHHT P I F+ + +W
Sbjct: 200 IAVVFLFAAIAFPALIITTGVWGFVKFWLMPWLVYHFWMSTFTIVHHTIPEIRFRPAADW 259

Query: 336 NAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNE 395
           +AA+AQLNGTVHC YP+W+E+LCHDINVHIPHH+S  IPSYNLR AH SL+ENWG  L E
Sbjct: 260 SAAEAQLNGTVHCDYPRWVEVLCHDINVHIPHHLSVAIPSYNLRLAHGSLKENWGPFLYE 319

Query: 396 ASWNWRLMKTIMTICHVYNKEENYVAFDRLNK 427
            ++NW+LM+ I   CH+Y+ E  Y  F  L K
Sbjct: 320 RTFNWQLMQQISGQCHLYDPEHGYRTFGSLKK 350

BLAST of CsaV3_1G028900 vs. ExPASy TrEMBL
Match: A0A0A0LTB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G255170 PE=4 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.0e-270
Identity = 443/443 (100.00%), Postives = 443/443 (100.00%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120
           LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV
Sbjct: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRLNKEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of CsaV3_1G028900 vs. ExPASy TrEMBL
Match: A0A1S3B9S5 (omega-6 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487358 PE=4 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 6.5e-265
Identity = 431/443 (97.29%), Postives = 438/443 (98.87%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRLVDSMALFNGPH N ARTQKIASHCSPG LQVKGEILFQKKIKRQNYLLPVK+AAI
Sbjct: 1   MACRLVDSMALFNGPHQNLARTQKIASHCSPGTLQVKGEILFQKKIKRQNYLLPVKKAAI 60

Query: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120
           LRA+AVP APPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKD+VESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEFDSAP+LRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420
           NVHIPHH+SPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT+CHVY+KEENYVA
Sbjct: 361 NVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRLNKEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of CsaV3_1G028900 vs. ExPASy TrEMBL
Match: A0A6J1EQE5 (omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436860 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 9.1e-259
Identity = 417/443 (94.13%), Postives = 433/443 (97.74%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRL DS+ LF GPH N+ARTQKIASH +PGIL VKG+I+FQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120
           LRA+AVP APPSSSAD AEYRKQLSESYGFEQIGEPLP+NVRLKD++ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASY+LGL+MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDSAPLLRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVY+KEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRL  EDSYPITFLKKVMPDLA
Sbjct: 421 FDRLKPEDSYPITFLKKVMPDLA 443

BLAST of CsaV3_1G028900 vs. ExPASy TrEMBL
Match: A0A5D3BV55 (Omega-6 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00370 PE=4 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 3.8e-257
Identity = 418/430 (97.21%), Postives = 425/430 (98.84%), Query Frame = 0

Query: 14  GPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAILRAIAVPAAPPSS 73
           GPH N ARTQKIASHCSPG LQVKGEILFQKKIKRQNYLLPVK+AAILRA+AVP APPSS
Sbjct: 18  GPHQNLARTQKIASHCSPGTLQVKGEILFQKKIKRQNYLLPVKKAAILRAVAVPVAPPSS 77

Query: 74  SADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDVKAWKSVLVSVASY 133
           SADSAEYRKQLSESYGFEQIGEPLPDNVRLKD+VESLPKEVFEINDVKAWKSVLVSVASY
Sbjct: 78  SADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDVKAWKSVLVSVASY 137

Query: 134 SLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLI 193
           SLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLI
Sbjct: 138 SLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLI 197

Query: 194 YPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIAHW 253
           YPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEFDSAP+LRKAIIYGYGPFRPWMSIAHW
Sbjct: 198 YPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYGYGPFRPWMSIAHW 257

Query: 254 LLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHFWM 313
           LLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHFWM
Sbjct: 258 LLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHFWM 317

Query: 314 STFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIP 373
           STFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHH+SPRIP
Sbjct: 318 STFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPRIP 377

Query: 374 SYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVAFDRLNKEDSYPIT 433
           SYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT+CHVY+KEENYVAFDRLNKEDSYPIT
Sbjct: 378 SYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVAFDRLNKEDSYPIT 437

Query: 434 FLKKVMPDLA 444
           FLKKVMPDLA
Sbjct: 438 FLKKVMPDLA 447

BLAST of CsaV3_1G028900 vs. ExPASy TrEMBL
Match: A0A6J1HLM6 (omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111465334 PE=4 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 6.5e-257
Identity = 414/443 (93.45%), Postives = 432/443 (97.52%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRL DS+ LF GPH N+ARTQKIASH +PGIL VKG+I+FQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120
           LRA+AVP APPSSSAD AEYRKQLSESYGFEQIGEPLP+NVRLKD++ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASY+LGLV+IAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVVIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDSAPLLRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSL+ENWGKHLNEASWNWRLMKTIMTICHVY+KEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLKENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRL  EDSYPI FL+KVMPDLA
Sbjct: 421 FDRLKPEDSYPIKFLRKVMPDLA 443

BLAST of CsaV3_1G028900 vs. TAIR 10
Match: AT4G30950.1 (fatty acid desaturase 6 )

HSP 1 Score: 708.8 bits (1828), Expect = 2.8e-204
Identity = 330/447 (73.83%), Postives = 382/447 (85.46%), Query Frame = 0

Query: 1   MACRLVDSMALFNGPHHNFARTQKIA---SHCSPGILQVKG-EILFQKKIKR-QNYLLPV 60
           MA R+ DS+  F GP     R  K+A   +  SPG+  VK  ++L + +  R +  + PV
Sbjct: 1   MASRIADSLFAFTGPQQCLPRVPKLAASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPV 60

Query: 61  K-RAAILRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEV 120
           K R   ++A+A P APP  SADSAE R+QL+ESYGF QIGE LP+NV LKD++++LPKEV
Sbjct: 61  KRRIGCIKAVAAPVAPP--SADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEV 120

Query: 121 FEINDVKAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 180
           FEI+D+KA KSVL+SV SY+LGL MIAK+PWYLLPLAWAWTGTAITGFFVIGHDCAHKSF
Sbjct: 121 FEIDDLKALKSVLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 180

Query: 181 SRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLR 240
           S+NKLVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S+P++R
Sbjct: 181 SKNKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMR 240

Query: 241 KAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTG 300
           KAII+GYGP RPW+SIAHW+ WHF+LKKFR +EV RVKISLACVFAFM +GWPLI+YK G
Sbjct: 241 KAIIFGYGPIRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVG 300

Query: 301 ILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIE 360
           ILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIE
Sbjct: 301 ILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIE 360

Query: 361 ILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNK 420
           ILCHDINVHIPHHISPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY+K
Sbjct: 361 ILCHDINVHIPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDK 420

Query: 421 EENYVAFDRLNKEDSYPITFLKKVMPD 442
           EENY+ FDRL  E+S PITFLKK MP+
Sbjct: 421 EENYIPFDRLAPEESQPITFLKKAMPN 445

BLAST of CsaV3_1G028900 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 130.2 bits (326), Expect = 4.2e-30
Identity = 97/344 (28.20%), Postives = 155/344 (45.06%), Query Frame = 0

Query: 94  GEPLPDNVRLKDVVESLPKEVFEINDVKAWKSVLVSVASYSLGLVMIAKAP----WYLLP 153
           G P P N  L D+  ++PK  +  N    WKS+   V   ++   + A A     W + P
Sbjct: 91  GAPPPFN--LADIRAAIPKHCWVKN---PWKSLSYVVRDVAIVFALAAGAAYLNNWIVWP 150

Query: 154 LAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAK 213
           L W   GT     FV+GHDC H SFS +  +  +VG L    ++ PY  WR  H  HH  
Sbjct: 151 LYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQN 210

Query: 214 TNMLEEDTAWHPVWKEEFD-----------SAPLLRKAIIYGYGPFRPWMSIAHWLLWHF 273
              +E D +WHP+ ++ ++           + PL+  A  +      P    +H   +H 
Sbjct: 211 HGHVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSH---YHP 270

Query: 274 DLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHFWMSTFTM 333
           D   F P E K V  S AC  A   +    + +  G +  +K + +P+     W+   T 
Sbjct: 271 DSDLFLPKERKDVLTSTACWTAMAAL-LVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTY 330

Query: 334 VHHTA--PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYN 393
           +HH      +P+   +EW+  +  L  T+   Y   I  + HDI  H+ HH+ P+IP Y+
Sbjct: 331 LHHHGHEDKLPWYRGKEWSYLRGGLT-TLDRDY-GLINNIHHDIGTHVIHHLFPQIPHYH 390

Query: 394 LRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 421
           L  A ++ +   GK+  E   +  L   ++ I     KE++YV+
Sbjct: 391 LVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVS 423

BLAST of CsaV3_1G028900 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-29
Identity = 86/334 (25.75%), Postives = 160/334 (47.90%), Query Frame = 0

Query: 102 RLKDVVESLPKEVFEINDVKAWKSV---LVSVASYSLGLVMIAKAPWYLLPLAWAWTGTA 161
           ++ D+  ++PK  +  + +++   V   +++VA+ ++  V +    W+L PL WA  GT 
Sbjct: 35  KIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAALAIAAVYVDS--WFLWPLYWAAQGTL 94

Query: 162 ITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTA 221
               FV+GHDC H SFS   L+  +VG +    ++ PY  WR  H  HH     +E D +
Sbjct: 95  FWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTHHQNHGHVENDES 154

Query: 222 WHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIAHWLLW--------HFD--LKKFRPNEV 281
           W P+ +  +   P   + + Y      P ++   +L +        HF+     F P+E 
Sbjct: 155 WVPLPERVYKKLPHSTRMLRYTVP--LPMLAYPLYLCYRSPGKEGSHFNPYSSLFAPSER 214

Query: 282 KRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHFWMSTFTMVHHTA--PHI 341
           K +  S  C ++ MF+    + +  G L  +K + +P++ +  W+   T +HH      +
Sbjct: 215 KLIATSTTC-WSIMFVSLIALSFVFGPLAVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKL 274

Query: 342 PFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQE 401
           P+   +EW+  +  L  T+   Y  +  I  HDI  H+ HH+ P+IP Y+L  A K+ + 
Sbjct: 275 PWYRGKEWSYLRGGLT-TIDRDYGIFNNI-HHDIGTHVIHHLFPQIPHYHLVDATKAAKH 334

Query: 402 NWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 421
             G++  E   +  +   ++       K+++YV+
Sbjct: 335 VLGRYYREPKTSGAIPIHLVESLVASIKKDHYVS 361

BLAST of CsaV3_1G028900 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-28
Identity = 91/312 (29.17%), Postives = 139/312 (44.55%), Query Frame = 0

Query: 94  GEPLPDNVRLKDVVESLPKEVFEINDVKAWKSVLVSVA-SYSLGLVMIAKAPWYLLPLAW 153
           G P P N  L D+  ++PK  +  N   +   V+  VA  + L  V      W L PL W
Sbjct: 84  GAPPPFN--LADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYW 143

Query: 154 AWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNM 213
              GT     FV+GHDC H SFS +  +  + G L    ++ PY  WR  H  HH     
Sbjct: 144 FAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHGH 203

Query: 214 LEEDTAWHP----VWKEEFDSAPLLRKAIIYGY--GPFRPWMSI--AHWLLWHFDLKKFR 273
           +E D +WHP    ++K    +  + R  + +     PF  W          +H D   F 
Sbjct: 204 VENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFL 263

Query: 274 PNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHFWMSTFTMVHHTA- 333
           P E K V  S AC  A   +    + +  G +  +K + +P+  +  W+   T +HH   
Sbjct: 264 PKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 323

Query: 334 -PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHK 393
              +P+   +EW+  +  L  T+   Y  WI  + HDI  H+ HH+ P+IP Y+L  A +
Sbjct: 324 EDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHLVEATE 383

Query: 394 SLQENWGKHLNE 395
           + +   GK+  E
Sbjct: 384 AAKPVLGKYYRE 390

BLAST of CsaV3_1G028900 vs. TAIR 10
Match: AT5G05580.2 (fatty acid desaturase 8 )

HSP 1 Score: 120.9 bits (302), Expect = 2.5e-27
Identity = 87/295 (29.49%), Postives = 131/295 (44.41%), Query Frame = 0

Query: 94  GEPLPDNVRLKDVVESLPKEVFEINDVKAWKSVLVSVA-SYSLGLVMIAKAPWYLLPLAW 153
           G P P N  L D+  ++PK  +  N   +   V+  VA  + L  V      W L PL W
Sbjct: 84  GAPPPFN--LADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYW 143

Query: 154 AWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNM 213
              GT     FV+GHDC H SFS +  +  + G L    ++ PY  WR  H  HH     
Sbjct: 144 FAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHGH 203

Query: 214 LEEDTAWHP----VWKEEFDSAPLLRKAIIYGY--GPFRPWMSI--AHWLLWHFDLKKFR 273
           +E D +WHP    ++K    +  + R  + +     PF  W          +H D   F 
Sbjct: 204 VENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFL 263

Query: 274 PNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHFWMSTFTMVHHTA- 333
           P E K V  S AC  A   +    + +  G +  +K + +P+  +  W+   T +HH   
Sbjct: 264 PKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 323

Query: 334 -PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNL 378
              +P+   +EW+  +  L  T+   Y  WI  + HDI  H+ HH+ P+IP Y+L
Sbjct: 324 EDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHL 373

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146685.12.1e-270100.00omega-6 fatty acid desaturase, chloroplastic isoform X1 [Cucumis sativus] >KGN65... [more]
XP_038880078.11.6e-26597.52omega-6 fatty acid desaturase, chloroplastic [Benincasa hispida][more]
XP_008443861.11.3e-26497.29PREDICTED: omega-6 fatty acid desaturase, chloroplastic [Cucumis melo][more]
XP_022930407.11.9e-25894.13omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita moschata][more]
XP_023529482.14.2e-25893.91omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P486271.8e-20875.51Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1[more]
P463124.0e-20373.83Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P486286.8e-20376.12Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1[more]
P486297.1e-18474.36Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD... [more]
P203888.3e-10853.01Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ... [more]
Match NameE-valueIdentityDescription
A0A0A0LTB11.0e-270100.00FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G2551... [more]
A0A1S3B9S56.5e-26597.29omega-6 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1EQE59.1e-25994.13omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 ... [more]
A0A5D3BV553.8e-25797.21Omega-6 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A6J1HLM66.5e-25793.45omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN... [more]
Match NameE-valueIdentityDescription
AT4G30950.12.8e-20473.83fatty acid desaturase 6 [more]
AT3G11170.14.2e-3028.20fatty acid desaturase 7 [more]
AT2G29980.11.2e-2925.75fatty acid desaturase 3 [more]
AT5G05580.11.3e-2829.17fatty acid desaturase 8 [more]
AT5G05580.22.5e-2729.49fatty acid desaturase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 145..392
e-value: 2.2E-30
score: 106.3
NoneNo IPR availablePANTHERPTHR32100:SF67BNAC03G67820D PROTEINcoord: 1..443
NoneNo IPR availablePANTHERPTHR32100OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTICcoord: 1..443
NoneNo IPR availableCDDcd03507Delta12-FADS-likecoord: 121..377
e-value: 1.08148E-80
score: 246.754

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G028900.1CsaV3_1G028900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process