CsaV3_1G007770 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_1G007770
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionCytochrome P450
Locationchr1: 4929995 .. 4932732 (+)
RNA-Seq ExpressionCsaV3_1G007770
SyntenyCsaV3_1G007770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACCCTCTCACTTATACTATTCAACATCATCTATAAAAGCCACTCCCATAACTTCTTCTTCTTCTATCCATTCCTTTCAATAATTTAGCCTCGTCATTCCGAGCTCGTCTTTTGATCGGAAAGTAAGATAAAGATCACAATGTGGACGATCTTGCTCGGTTTGGCGACGTTGGCAATTGCCTACTATATTCATTGGGTTAACAAATGGAAGGATTCTAAATTCAACGGAGTTTTGCCGCCGGGCACCATGGGGCTGCCCCTCATCGGAGAAACCATTCAACTTAGTCGCCCTAGTGACTCCCTTGATGTTCATCCTTTCATTCAACGCAAAGTTAAAAGGTATTCTTTTATTGTTATATAAAAAGTATTATAAACATGCATATATATTATTTTCACTAGAGAAAACTGTGGACGAAGTTTTTTTGGATTTAAATTCTACATAAATAAATTTTTAGATATGGACCGATCTTCAAGACTTGTTTGGCGGGAAGGCCGGTGGTGGTTTCAACGGATGCAGAGTTTAACCATTACATAATGCTCCAAGAAGGAAGGGCCGTAGAAATGTGGTATTTGGATACACTCTCTAAATTCTTTGGCCTTGACACTGAATGGCTCAAAGCCCTTGGCCTCATCCACAAATACATTAGAAGCATTACTTTGAACCACTTTGGTGCTGAGTCCCTTCGTGAGCGTTTCCTTCCTCGTATCGAAGAATCCGCTCGAGAAACCCTTCATTATTGGTCAACTCAAACCAGCGTTGAAGTCAAGGAATCAGCCGCTGCGGTAGGGATACGTTTGATAATTAAACATATCATTTAAATCTATATGATTTTAATTGTTTAAACAACTTAACAATTATTCTTTTATGTGATTTTTCAGATGGTTTTCAGAACTTCGATTGTTAAGATGTTTAGTGAAGATTCTAGTAAATTACTGACAGAAGGTCTCACTAAGAAGTTCACAGGACTTCTCGGAGGTTTTCTCACCTTGCCTCTAAATTTGCCTGGCACTACCTATCATAAATGCATAAAGGTAAATAATCAATTTTTAAACAACTAAAAAAGAGATATATGCATATACATGTATATCATTATTATAAATATATAATTTATTTTCTATTGACATTAGGACATGAAGCAAATCCAAAAGAAGCTAAAAGACATTTTAGAGGAAAGATTGGCTAAAGGGGTTAAAATTGATGAAGATTTCTTGGGGCAAGCCATTAAAGATAAAGAATCTCAACAATTCATTTCAGAGGAATTCATTATCCAGTTGTTGTTTTCCATCAGCTTTGCTAGCTTTGAGTCCATCTCTACCACTCTTACTTTGATTCTCAACTTCCTCGCCGATCACCCCGACGTAGTGAAAGAATTGGAGGTAAGACTCGAAAAACATACAAAAAGAAAATTATTATTTAATGAGACAACTGAAACAATGCGATGGATGTACGATGAGAAAATAAAATCTAATGTTTTCATTAAATTTTACATCTATCGATTACGAACGGACAACGTCGTAAAAGTCGTATTCAATAAACGATTTTAATTATATATTTTTGTGTTGATTAGGCTGAGCATGAGGCTATTAGAAAGGCAAGGGCAGATCCAGATGGACCAATCACTTGGGAAGAATACAAATCCATGAATTTCACACTCAATGTAATTCTATAATATTATATTTCTAAATCTCTAAGAACACACATTATTATATATTTCAATTTACTTCTTGTCCATATTCCTTTTTTATTTAACATAATGTGATGATCACATTGTTTTCCTTTGGTCTATTTTGGATTTTAGGTCATCTGTGAAACACTTAGGTTGGGAAGTGTAACACCTGCTTTGTTGAGGAAGACAACCAAGGAAATTCAAATAAAAGGTAAAATCAAAAGTTTATATACTTTGTCGTATAAATTAATCGTGAAGTTATTAACTTAGATCATTTAATTGATGTTATAGGATACACAATTCCAGAAGGATGGACAGTAATGCTTGTGACCGCTTCTCGTCATAGAGATCCAGAAGTGTACAAGGATCCCGATACCTTCAATCCATGGCGTTGGAAGGTATATTATAGCATAGAAGAAAAAAAGCTCTTAACTAAATTGTGTTAATTAATTAATTGGATAATTAAGTTTTAATAATGGTGATTATGAGTACATATTATAGGAGTTGGACTCAATTACTATTCAAAAGAACTTCATGCCATTTGGGGGAGGCTTAAGGCATTGTGCTGGTGCTGAATACTCTAAAGTCTATTTGTGCACTTTCCTTCATATCCTTTTCACCAAATACAGGTACACTTTATAAATCAAATCTACTAATTATTTTTAACATATAGTATACTAATTAAGAGAGTTTAATTATACAATAATCTTTCTTTTTTAATTCTGATAGATGGAGAAAACTAAAGGGAGGAAAGATTGCAAGGGCTCATATATTGAGGTTTGAAGATGGGTTATATGTGAACTTCACTCCCAAGGAATGATTGAATGGAATTTGTTGCTTTACTAAAAAAGAAATGTTATTTATCTAAACTTTATGAAATGATGAACTACTTGTACTAGCTAGCTATCATATTATGATCATATGGTTCAATAAGACCAAGTTCTTCTTCTTGTATTAAAGGCAATCTTATGTAAAATCAAGTTCCATTAATAGCATGGTTTCATTAATGGCCTTTCAAATTTAACCTTTTAAAAATATATTCGAGTATAAGTATTCTTTTTAAATAATAA

mRNA sequence

ATGTGGACGATCTTGCTCGGTTTGGCGACGTTGGCAATTGCCTACTATATTCATTGGGTTAACAAATGGAAGGATTCTAAATTCAACGGAGTTTTGCCGCCGGGCACCATGGGGCTGCCCCTCATCGGAGAAACCATTCAACTTAGTCGCCCTAGTGACTCCCTTGATGTTCATCCTTTCATTCAACGCAAAGTTAAAAGATATGGACCGATCTTCAAGACTTGTTTGGCGGGAAGGCCGGTGGTGGTTTCAACGGATGCAGAGTTTAACCATTACATAATGCTCCAAGAAGGAAGGGCCGTAGAAATGTGGTATTTGGATACACTCTCTAAATTCTTTGGCCTTGACACTGAATGGCTCAAAGCCCTTGGCCTCATCCACAAATACATTAGAAGCATTACTTTGAACCACTTTGGTGCTGAGTCCCTTCGTGAGCGTTTCCTTCCTCGTATCGAAGAATCCGCTCGAGAAACCCTTCATTATTGGTCAACTCAAACCAGCGTTGAAGTCAAGGAATCAGCCGCTGCGATGGTTTTCAGAACTTCGATTGTTAAGATGTTTAGTGAAGATTCTAGTAAATTACTGACAGAAGGTCTCACTAAGAAGTTCACAGGACTTCTCGGAGGTTTTCTCACCTTGCCTCTAAATTTGCCTGGCACTACCTATCATAAATGCATAAAGGACATGAAGCAAATCCAAAAGAAGCTAAAAGACATTTTAGAGGAAAGATTGGCTAAAGGGGTTAAAATTGATGAAGATTTCTTGGGGCAAGCCATTAAAGATAAAGAATCTCAACAATTCATTTCAGAGGAATTCATTATCCAGTTGTTGTTTTCCATCAGCTTTGCTAGCTTTGAGTCCATCTCTACCACTCTTACTTTGATTCTCAACTTCCTCGCCGATCACCCCGACGTAGTGAAAGAATTGGAGGCTGAGCATGAGGCTATTAGAAAGGCAAGGGCAGATCCAGATGGACCAATCACTTGGGAAGAATACAAATCCATGAATTTCACACTCAATGTCATCTGTGAAACACTTAGGTTGGGAAGTGTAACACCTGCTTTGTTGAGGAAGACAACCAAGGAAATTCAAATAAAAGGATACACAATTCCAGAAGGATGGACAGTAATGCTTGTGACCGCTTCTCGTCATAGAGATCCAGAAGTGTACAAGGATCCCGATACCTTCAATCCATGGCGTTGGAAGGAGTTGGACTCAATTACTATTCAAAAGAACTTCATGCCATTTGGGGGAGGCTTAAGGCATTGTGCTGGTGCTGAATACTCTAAAGTCTATTTGTGCACTTTCCTTCATATCCTTTTCACCAAATACAGATGGAGAAAACTAAAGGGAGGAAAGATTGCAAGGGCTCATATATTGAGGTTTGAAGATGGGTTATATGTGAACTTCACTCCCAAGGAATGA

Coding sequence (CDS)

ATGTGGACGATCTTGCTCGGTTTGGCGACGTTGGCAATTGCCTACTATATTCATTGGGTTAACAAATGGAAGGATTCTAAATTCAACGGAGTTTTGCCGCCGGGCACCATGGGGCTGCCCCTCATCGGAGAAACCATTCAACTTAGTCGCCCTAGTGACTCCCTTGATGTTCATCCTTTCATTCAACGCAAAGTTAAAAGATATGGACCGATCTTCAAGACTTGTTTGGCGGGAAGGCCGGTGGTGGTTTCAACGGATGCAGAGTTTAACCATTACATAATGCTCCAAGAAGGAAGGGCCGTAGAAATGTGGTATTTGGATACACTCTCTAAATTCTTTGGCCTTGACACTGAATGGCTCAAAGCCCTTGGCCTCATCCACAAATACATTAGAAGCATTACTTTGAACCACTTTGGTGCTGAGTCCCTTCGTGAGCGTTTCCTTCCTCGTATCGAAGAATCCGCTCGAGAAACCCTTCATTATTGGTCAACTCAAACCAGCGTTGAAGTCAAGGAATCAGCCGCTGCGATGGTTTTCAGAACTTCGATTGTTAAGATGTTTAGTGAAGATTCTAGTAAATTACTGACAGAAGGTCTCACTAAGAAGTTCACAGGACTTCTCGGAGGTTTTCTCACCTTGCCTCTAAATTTGCCTGGCACTACCTATCATAAATGCATAAAGGACATGAAGCAAATCCAAAAGAAGCTAAAAGACATTTTAGAGGAAAGATTGGCTAAAGGGGTTAAAATTGATGAAGATTTCTTGGGGCAAGCCATTAAAGATAAAGAATCTCAACAATTCATTTCAGAGGAATTCATTATCCAGTTGTTGTTTTCCATCAGCTTTGCTAGCTTTGAGTCCATCTCTACCACTCTTACTTTGATTCTCAACTTCCTCGCCGATCACCCCGACGTAGTGAAAGAATTGGAGGCTGAGCATGAGGCTATTAGAAAGGCAAGGGCAGATCCAGATGGACCAATCACTTGGGAAGAATACAAATCCATGAATTTCACACTCAATGTCATCTGTGAAACACTTAGGTTGGGAAGTGTAACACCTGCTTTGTTGAGGAAGACAACCAAGGAAATTCAAATAAAAGGATACACAATTCCAGAAGGATGGACAGTAATGCTTGTGACCGCTTCTCGTCATAGAGATCCAGAAGTGTACAAGGATCCCGATACCTTCAATCCATGGCGTTGGAAGGAGTTGGACTCAATTACTATTCAAAAGAACTTCATGCCATTTGGGGGAGGCTTAAGGCATTGTGCTGGTGCTGAATACTCTAAAGTCTATTTGTGCACTTTCCTTCATATCCTTTTCACCAAATACAGATGGAGAAAACTAAAGGGAGGAAAGATTGCAAGGGCTCATATATTGAGGTTTGAAGATGGGTTATATGTGAACTTCACTCCCAAGGAATGA

Protein sequence

MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPFIQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWLKALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFRTSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDILEERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLADHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTTKEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGLRHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE*
Homology
BLAST of CsaV3_1G007770 vs. NCBI nr
Match: NP_001289636.1 (Cytochrome P450 87A3-like [Cucumis sativus] >AIT72037.1 cytochrome P450 [Cucumis sativus] >KGN64240.1 hypothetical protein Csa_013216 [Cucumis sativus])

HSP 1 Score: 966.1 bits (2496), Expect = 1.1e-277
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL
Sbjct: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA
Sbjct: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL
Sbjct: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 474

BLAST of CsaV3_1G007770 vs. NCBI nr
Match: XP_008437370.1 (PREDICTED: cytochrome P450 87A3-like [Cucumis melo] >TYJ99092.1 cytochrome P450 87A3-like [Cucumis melo var. makuwa])

HSP 1 Score: 952.2 bits (2460), Expect = 1.7e-273
Identity = 466/474 (98.31%), Postives = 469/474 (98.95%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQ KVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQ SVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQPSVEVKESAAAMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TSIVKMFSEDSSKLLT GLTKKFTGLLGGFLTLPLN+PGTTYHKCIKDMK+IQKKLKDIL
Sbjct: 181 TSIVKMFSEDSSKLLTAGLTKKFTGLLGGFLTLPLNVPGTTYHKCIKDMKEIQKKLKDIL 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           EERLAKGV IDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA
Sbjct: 241 EERLAKGVSIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           DHPDV KELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT
Sbjct: 301 DHPDVAKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQ+NFMPFGGGL
Sbjct: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQRNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 474

BLAST of CsaV3_1G007770 vs. NCBI nr
Match: XP_038895732.1 (cucurbitadienol 11-hydroxylase [Benincasa hispida])

HSP 1 Score: 920.2 bits (2377), Expect = 7.1e-264
Identity = 449/474 (94.73%), Postives = 461/474 (97.26%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTILLGLAT+A+ YYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATVAVVYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQ KVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLR+RFLPRIEESARETLHYWSTQ SVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRDRFLPRIEESARETLHYWSTQPSVEVKESAAAMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TSIVKMFSEDSSKLLT GLTKKFTGLLGGFLTLPLNLPGTTY+KC+KDMK+IQKKLKDIL
Sbjct: 181 TSIVKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMKDMKEIQKKLKDIL 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           EERLAKG   +EDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA
Sbjct: 241 EERLAKGASSEEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           DHP+VVKELEAEHEAIRKARADPDGPITWEEYKSM FTLNVI ETLRLGSVTPALLRKTT
Sbjct: 301 DHPEVVKELEAEHEAIRKARADPDGPITWEEYKSMTFTLNVISETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           KEIQIKGY IPEGWTVMLVTASRHRDPEVYKDP TFNPWRWKE+DS+T+QKNFMPFGGGL
Sbjct: 361 KEIQIKGYIIPEGWTVMLVTASRHRDPEVYKDPATFNPWRWKEIDSMTVQKNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHILFTKYRW KLKGGKIARAHIL FEDGL+VNFTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILFTKYRWTKLKGGKIARAHILSFEDGLHVNFTPKE 474

BLAST of CsaV3_1G007770 vs. NCBI nr
Match: KAG6573008.1 (Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 880.2 bits (2273), Expect = 8.2e-252
Identity = 423/474 (89.24%), Postives = 452/474 (95.36%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MW I++GLATLA+AYYIHW+NKWKDSKFNGVLPPGTMGLPL+GET+QL+RPSDSLDVHPF
Sbjct: 1   MWAIVVGLATLAVAYYIHWINKWKDSKFNGVLPPGTMGLPLVGETLQLARPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           I++KVKRYGPIFKTCLAGRPVVVSTDAEFN+YIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IKKKVKRYGPIFKTCLAGRPVVVSTDAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALG IHKYIRSITLNHFGAESLRERFLPRIEESA+ETL YW+TQ SVEVK+SAA MVFR
Sbjct: 121 KALGFIHKYIRSITLNHFGAESLRERFLPRIEESAKETLRYWATQPSVEVKDSAAVMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TS+VKM SEDSSKLLT GLTKKFTGLLGGFLTLP+N+PGTTY+KC+KDMK+IQKKL++IL
Sbjct: 181 TSMVKMVSEDSSKLLTGGLTKKFTGLLGGFLTLPINVPGTTYNKCMKDMKEIQKKLREIL 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           E RLA G   DEDFLGQAIKDK SQQFIS++FIIQLLFSISFASFESISTTLTL+LN+LA
Sbjct: 241 EGRLASGAGSDEDFLGQAIKDKGSQQFISDDFIIQLLFSISFASFESISTTLTLVLNYLA 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           DHPDVVKELEAEHEAIR ARADPDGPITWEEYKSM FTL+VI ETLRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRNARADPDGPITWEEYKSMTFTLHVIFETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           KE+QI GYTIPEGWTVMLVTASRHRDP VYKDP TFNPWRWKELDSITIQKNFMPFGGGL
Sbjct: 361 KELQINGYTIPEGWTVMLVTASRHRDPAVYKDPHTFNPWRWKELDSITIQKNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHILFTKYRW KLKGGK+ARAHIL FEDGL+V FTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILFTKYRWTKLKGGKVARAHILSFEDGLHVKFTPKE 474

BLAST of CsaV3_1G007770 vs. NCBI nr
Match: KAG7012193.1 (Cytochrome P450 87A3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 879.8 bits (2272), Expect = 1.1e-251
Identity = 423/474 (89.24%), Postives = 452/474 (95.36%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MW I++GLATLA+AYYIHW+NKWKDSKFNGVLPPGTMGLPL+GET+QL+RPSDSLDVHPF
Sbjct: 1   MWAIVVGLATLAVAYYIHWINKWKDSKFNGVLPPGTMGLPLVGETLQLARPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           I++KVKRYGPIFKTCLAGRPVVVSTDAEFN+YIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IKKKVKRYGPIFKTCLAGRPVVVSTDAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALG IHKYIRSITLNHFGAESLRERFLPRIEESA+ETL YW+TQ SVEVK+SAA MVFR
Sbjct: 121 KALGFIHKYIRSITLNHFGAESLRERFLPRIEESAKETLRYWATQPSVEVKDSAAVMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TS+VKM SEDSSKLLT GLTKKFTGLLGGFLTLP+N+PGTTY+KC+KDMK+IQKKL++IL
Sbjct: 181 TSMVKMASEDSSKLLTGGLTKKFTGLLGGFLTLPINVPGTTYNKCMKDMKEIQKKLREIL 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           E RLA G   DEDFLGQAIKDK SQQFIS++FIIQLLFSISFASFESISTTLTL+LN+LA
Sbjct: 241 EGRLASGAGSDEDFLGQAIKDKGSQQFISDDFIIQLLFSISFASFESISTTLTLVLNYLA 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           DHPDVVKELEAEHEAIR ARADPDGPITWEEYKSM FTL+VI ETLRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRNARADPDGPITWEEYKSMTFTLHVIFETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           KE+QI GYTIPEGWTVMLVTASRHRDP VYKDP TFNPWRWKELDSITIQKNFMPFGGGL
Sbjct: 361 KELQINGYTIPEGWTVMLVTASRHRDPAVYKDPHTFNPWRWKELDSITIQKNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHILFTKYRW KLKGGK+ARAHIL FEDGL+V FTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILFTKYRWTKLKGGKVARAHILSFEDGLHVKFTPKE 474

BLAST of CsaV3_1G007770 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 5.0e-236
Identity = 395/474 (83.33%), Postives = 432/474 (91.14%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWT++LGLATL +AYYIHW+NKW+DSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTVVLGLATLFVAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQ+KV+RYGPIFKTCLAGRPVVVS DAEFN+YIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQKKVERYGPIFKTCLAGRPVVVSADAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAE+LRERFLP IE S+ E LH WSTQ SVEVK ++A MVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAEALRERFLPFIEASSMEALHSWSTQPSVEVKNASALMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TS+ KMF ED+ K L+  +  KFT LLGGFL+LPLN PGTTYHKC+KDMK+IQKKL++++
Sbjct: 181 TSVNKMFGEDAKK-LSGNIPGKFTKLLGGFLSLPLNFPGTTYHKCLKDMKEIQKKLREVV 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           ++RLA      EDFLGQA+KDKES++FISEEFIIQLLFSISFASFESISTTLTLIL  L 
Sbjct: 241 DDRLANVGPDVEDFLGQALKDKESEKFISEEFIIQLLFSISFASFESISTTLTLILKLLD 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           +HP+VVKELEAEHEAIRKARADPDGPITWEEYKSM FTL VI ETLRLGSVTPALLRKT 
Sbjct: 301 EHPEVVKELEAEHEAIRKARADPDGPITWEEYKSMTFTLQVINETLRLGSVTPALLRKTV 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           K++Q+KGY IPEGWT+MLVTASRHRDP+VYKDP  FNPWRWK+LDSITIQKNFMPFGGGL
Sbjct: 361 KDLQVKGYIIPEGWTIMLVTASRHRDPKVYKDPHIFNPWRWKDLDSITIQKNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHIL TKYRW KL GG IARAHIL FEDGL+V FTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILCTKYRWTKLGGGTIARAHILSFEDGLHVKFTPKE 473

BLAST of CsaV3_1G007770 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 416.4 bits (1069), Expect = 4.4e-115
Identity = 198/471 (42.04%), Postives = 292/471 (62.00%), Query Frame = 0

Query: 5   LLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPFIQRK 64
           LL  A  A+   + W  +W   + NG LPPG++GLP+IGET+Q   P+ + D+ PF++ +
Sbjct: 44  LLCAALAAVVALLRWAYRWSHPRSNGRLPPGSLGLPVIGETLQFFAPNPTCDLSPFVKER 103

Query: 65  VKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWLKALG 124
           +KRYG IFKT + GRPVVVS D E N+Y+  QEG+  E WY DT ++ FG D       G
Sbjct: 104 IKRYGSIFKTSVVGRPVVVSADPEMNYYVFQQEGKLFESWYPDTFTEIFGRDNVG-SLHG 163

Query: 125 LIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFRTSIV 184
            ++KY++++ L  +G E+L+   L   + + R +L  W++Q SVE+KE  + M+F  +  
Sbjct: 164 FMYKYLKTLVLRLYGQENLKSVLLAETDAACRGSLASWASQPSVELKEGISTMIFDLTAK 223

Query: 185 KMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDILEERL 244
           K+   D SK     L K F   + G ++ PLN+PGT YH+C++  K   K L+ +++ER+
Sbjct: 224 KLIGYDPSKPSQVNLRKNFGAFICGLISFPLNIPGTAYHECMEGRKNAMKVLRGMMKERM 283

Query: 245 AKGVKIDEDFLGQAIKD-KESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLADHP 304
           A+  +  EDF    I++ +  +  ++E   + L+F + FASFE+ +  LT+ +  L ++P
Sbjct: 284 AEPERPCEDFFDHVIQELRREKPLLTETIALDLMFVLLFASFETTALALTIGVKLLTENP 343

Query: 305 DVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTTKEI 364
            VV  L  EHEAI + R DP+  +TW EYKSM FT  VI E +RL ++ P + RK  +++
Sbjct: 344 KVVDALREEHEAIIRNRKDPNSGVTWAEYKSMTFTSQVIMEIVRLANIVPGIFRKALQDV 403

Query: 365 QIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSIT-IQKNFMPFGGGLRH 424
           +IKGYTIP GW +M+   + H +PE+Y+DP  FNPWRW+    IT   K+FM FGGGLR 
Sbjct: 404 EIKGYTIPAGWGIMVCPPAVHLNPEIYEDPLAFNPWRWQGKPEITGGTKHFMAFGGGLRF 463

Query: 425 CAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPK 474
           C G + SKV + TF+H L TKY WR +KGG I R   L F DG ++   PK
Sbjct: 464 CVGTDLSKVLMATFIHSLVTKYSWRTVKGGNIVRTPGLSFPDGFHIQLFPK 513

BLAST of CsaV3_1G007770 vs. ExPASy Swiss-Prot
Match: Q43147 (Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.7e-79
Identity = 159/448 (35.49%), Postives = 259/448 (57.81%), Query Frame = 0

Query: 22  KWKDSKFNGV-LPPGTMGLPLIGETIQLSRPSDSLDVHPFIQRKVKRYGPIFKTCLAGRP 81
           +W   K+N   LPPGTMG PL GET +  +   S     F++ +  RYG  FK+ + G P
Sbjct: 23  RWNQVKYNQKNLPPGTMGWPLFGETTEFLKLGPS-----FMKNQRARYGSFFKSHILGCP 82

Query: 82  VVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWLKALGLIHKYIRSITLNHFGA 141
            +VS D+E N YI++ E + +   Y  ++    G         G  HKY+R   L+    
Sbjct: 83  TIVSMDSELNRYILVNEAKGLVPGYPQSMIDILG-KCNIAAVNGSAHKYMRGALLSLISP 142

Query: 142 ESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFRTSIVKMFSEDSSKLLTEGLT 201
             +R++ LP+I+E  R  L  W  +  ++++E    M F +S+ ++   +S+ L  E ++
Sbjct: 143 TMIRDQLLPKIDEFMRSHLTNWDNKV-IDIQEKTNKMAFLSSLKQIAGIESTSLAQEFMS 202

Query: 202 KKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDILEERLAKGVKIDEDFLGQAIK 261
            +F  L+ G L+LP+NLP T YH+  +  K I   L+ ++EER A   +I  D LG  + 
Sbjct: 203 -EFFNLVLGTLSLPINLPNTNYHRGFQARKIIVNLLRTLIEERRA-SKEIQHDMLGYLMN 262

Query: 262 DKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLADHPDVVKELEAEHEAIRKAR 321
           ++ ++  ++++ +I L+ +I ++ +E++STT  + + +L DHP V++EL  EH AIR+ +
Sbjct: 263 EEATRFKLTDDEMIDLIITILYSGYETVSTTSMMAVKYLHDHPKVLEELRKEHMAIRE-K 322

Query: 322 ADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTTKEIQIKGYTIPEGWTVMLVT 381
             P+ PI + +Y+SM FT  VI ET RL ++   +LRKTT++++I GY IP+GW + + T
Sbjct: 323 KKPEDPIDYNDYRSMRFTRAVILETSRLATIVNGVLRKTTQDMEINGYIIPKGWRIYVYT 382

Query: 382 ASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGLRHCAGAEYSKVYLCTFLHIL 441
              + DP +Y DP +FNPWRW +  S+  Q +F+ FGGG R C G E     + TFLH  
Sbjct: 383 RELNYDPRLYPDPYSFNPWRWMD-KSLEHQNSFLVFGGGTRQCPGKELGVAEISTFLHYF 442

Query: 442 FTKYRWRKLKGGKIARAHILRFEDGLYV 469
            TKYRW ++ G K+ +   +   +GL +
Sbjct: 443 VTKYRWEEIGGDKLMKFPRVEAPNGLRI 459

BLAST of CsaV3_1G007770 vs. ExPASy Swiss-Prot
Match: Q69F95 (Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2)

HSP 1 Score: 283.9 bits (725), Expect = 3.4e-75
Identity = 164/470 (34.89%), Postives = 264/470 (56.17%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGV-LPPGTMGLPLIGETIQLSRPSDSLDVHP 60
           ++  +LG+  L +  +   + KW + +F    LPPG MG P+ GET +  +   +     
Sbjct: 3   LFMAILGVLVLLLC-FCSALLKWNEVRFRRKGLPPGAMGWPVFGETTEFLKQGPN----- 62

Query: 61  FIQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEW 120
           F++ K  RYG  FK+ + G P +VS D E N +I++ E + +   Y  ++    G  T  
Sbjct: 63  FMKNKRARYGSFFKSHILGCPTIVSMDPELNRFILMNEAKGLVPGYPQSMLDILG--TRN 122

Query: 121 LKAL-GLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMV 180
           + A+ G  HKY+R   L+      +R++ LP+I+E  R  L  W  +  + ++E    M 
Sbjct: 123 IAAVHGSTHKYMRGALLSIISPTLIRDQLLPKIDEFMRTHLMDWDNKV-INIQEKTKEMA 182

Query: 181 FRTSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKD 240
           F +S+ ++   +SS +    +T +F  L+ G L+LP+NLP T Y   ++  K I   L  
Sbjct: 183 FLSSLKQIAGMESSSIAQPFMT-EFFKLVLGTLSLPINLPRTNYRGGLQARKSIISILSQ 242

Query: 241 ILEERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNF 300
           +LEER A    +  D LG  +K  E++  +S+E II L+ +I ++ +E++STT  + + +
Sbjct: 243 LLEERKA-SQDVHVDMLGCLMKKDENRYKLSDEEIIDLVITIMYSGYETVSTTSMMAVKY 302

Query: 301 LADHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRK 360
           L DHP V++E+  EH AIR+ R  P+ PI   + KSM FT  VI ET RL ++   +LRK
Sbjct: 303 LHDHPKVLEEIRKEHFAIRE-RKKPEDPIDCNDLKSMRFTRAVIFETSRLATIVNGVLRK 362

Query: 361 TTKEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGG 420
           TT ++++ GY IP+GW + + T   + DP +Y DP TFNPWRW   +S+  Q +F+ FGG
Sbjct: 363 TTHDMELNGYLIPKGWRIYVYTREINYDPFLYHDPLTFNPWRWLG-NSLESQSHFLIFGG 422

Query: 421 GLRHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYV 469
           G R C G E     + TFLH   T+YRW ++ G K+ +   +   +GL++
Sbjct: 423 GTRQCPGKELGIAEISTFLHYFVTRYRWEEVGGDKLMKFPRVVAPNGLHI 459

BLAST of CsaV3_1G007770 vs. ExPASy Swiss-Prot
Match: Q50LE0 (Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.4e-73
Identity = 150/449 (33.41%), Postives = 251/449 (55.90%), Query Frame = 0

Query: 22  KWKDSKFNGV-LPPGTMGLPLIGETIQLSRPSDSLDVHPFIQRKVKRYGPIFKTCLAGRP 81
           +W D  +N   LPPGTMG P+ GET +      +     F++ +  RYG  FK+ + G P
Sbjct: 23  RWIDIVYNKKNLPPGTMGWPIFGETREFLNQGPN-----FMKNQRARYGNFFKSHILGCP 82

Query: 82  VVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWLKALGLIHKYIRSITLNHFGA 141
            VVS DA  N YI+  E + +   Y  ++    G         G  HKYIR   L+    
Sbjct: 83  TVVSMDAGLNVYILNNEAKGLIPGYPQSMLDILG-KCNIAAVHGATHKYIRGALLSLINP 142

Query: 142 ESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFRTSIVKMFSEDSSKLLTEGLT 201
             +++  LP+I++  R  L  W     +++++    M F +S+ ++    +S  + +   
Sbjct: 143 TMIKDHILPKIDKFMRSHLSGWDNCNVIDIQQMTKEMAFFSSLDQIGGFATSSSIAQEFR 202

Query: 202 KKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDILEERLAKGVKIDEDFLGQAIK 261
             F  +  G ++LP+N P T Y++ ++  K I K L+ I+E+R     KI +D LG  + 
Sbjct: 203 AGFLNIALGTISLPINFPTTNYYRGLQGRKTIVKLLRKIIEDRRG-SKKIQQDMLGLMMN 262

Query: 262 DKESQQF-ISEEFIIQLLFSISFASFESISTTLTLILNFLADHPDVVKELEAEHEAIRKA 321
           ++   ++ +S+E +I  + +I ++ FE++STT  + + +L DHP  ++E+  EH AIR+ 
Sbjct: 263 EEAKNRYTLSDEELIDQIITIMYSGFETVSTTSMMAVKYLHDHPKALEEIRKEHFAIREK 322

Query: 322 RADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTTKEIQIKGYTIPEGWTVMLV 381
           ++  D PI + ++K+M FT  VI ETLRL ++   +LRKTT+++++ GY IP+GW + + 
Sbjct: 323 KSLED-PIDYNDFKAMRFTRAVIYETLRLATIVNGVLRKTTQDMELNGYMIPKGWRIYVY 382

Query: 382 TASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGLRHCAGAEYSKVYLCTFLHI 441
           T   + DP +Y DP TFNPWRW E +++  Q +F+ FGGG R C G E     + TFLH 
Sbjct: 383 TRELNYDPLIYPDPYTFNPWRWLE-NNLDHQSSFLMFGGGTRLCPGKELGVAEISTFLHY 442

Query: 442 LFTKYRWRKLKGGKIARAHILRFEDGLYV 469
             T+YRW ++ G K+ +   +   +GL++
Sbjct: 443 FVTRYRWEEVGGNKLMKFPRVEALNGLWI 462

BLAST of CsaV3_1G007770 vs. ExPASy TrEMBL
Match: A0A0A0LQP6 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa1G044890 PE=2 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 5.5e-278
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL
Sbjct: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA
Sbjct: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL
Sbjct: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 474

BLAST of CsaV3_1G007770 vs. ExPASy TrEMBL
Match: A0A5D3BH60 (Cytochrome P450 87A3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002960 PE=3 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 8.2e-274
Identity = 466/474 (98.31%), Postives = 469/474 (98.95%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQ KVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQ SVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQPSVEVKESAAAMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TSIVKMFSEDSSKLLT GLTKKFTGLLGGFLTLPLN+PGTTYHKCIKDMK+IQKKLKDIL
Sbjct: 181 TSIVKMFSEDSSKLLTAGLTKKFTGLLGGFLTLPLNVPGTTYHKCIKDMKEIQKKLKDIL 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           EERLAKGV IDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA
Sbjct: 241 EERLAKGVSIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           DHPDV KELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT
Sbjct: 301 DHPDVAKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQ+NFMPFGGGL
Sbjct: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQRNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 474

BLAST of CsaV3_1G007770 vs. ExPASy TrEMBL
Match: A0A1S3AUF5 (cytochrome P450 87A3-like OS=Cucumis melo OX=3656 GN=LOC103482802 PE=3 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 8.2e-274
Identity = 466/474 (98.31%), Postives = 469/474 (98.95%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQ KVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQ SVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQPSVEVKESAAAMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TSIVKMFSEDSSKLLT GLTKKFTGLLGGFLTLPLN+PGTTYHKCIKDMK+IQKKLKDIL
Sbjct: 181 TSIVKMFSEDSSKLLTAGLTKKFTGLLGGFLTLPLNVPGTTYHKCIKDMKEIQKKLKDIL 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           EERLAKGV IDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA
Sbjct: 241 EERLAKGVSIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           DHPDV KELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT
Sbjct: 301 DHPDVAKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQ+NFMPFGGGL
Sbjct: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQRNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 474

BLAST of CsaV3_1G007770 vs. ExPASy TrEMBL
Match: A0A6J1GV28 (cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111457186 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.3e-251
Identity = 422/474 (89.03%), Postives = 451/474 (95.15%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MW I++GLATLA+AYYIHW+NKWKDSKFNGVLPPGTMGLPL+GET+QL+RPSDSLDVHPF
Sbjct: 1   MWAIVVGLATLAVAYYIHWINKWKDSKFNGVLPPGTMGLPLVGETLQLARPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           I++KVKRYG IFKTCLAGRPVVVSTDAEFN+YIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IKKKVKRYGSIFKTCLAGRPVVVSTDAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALG IHKYIRSITLNHFGAESLRERFLPRIEESA+ETL YW+TQ SVEVK+SAA MVFR
Sbjct: 121 KALGFIHKYIRSITLNHFGAESLRERFLPRIEESAKETLRYWATQPSVEVKDSAAVMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TS+VKM SEDSSKLLT GLTKKFTGLLGGFLTLP+N+PGTTY+KC+KDMK+IQKKL++IL
Sbjct: 181 TSMVKMVSEDSSKLLTGGLTKKFTGLLGGFLTLPINVPGTTYNKCMKDMKEIQKKLREIL 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           E RLA G   DEDFLGQAIKDK SQQFIS++FIIQLLFSISFASFESISTTLTL+LN+LA
Sbjct: 241 EGRLASGAGSDEDFLGQAIKDKGSQQFISDDFIIQLLFSISFASFESISTTLTLVLNYLA 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           DHPDVVKELEAEHEAIR ARADPDGPITWEEYKSM FTL+VI ETLRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRNARADPDGPITWEEYKSMTFTLHVIFETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           KE+QI GYTIPEGWTVMLVTASRHRDP VYKDP TFNPWRWKELDSITIQKNFMPFGGGL
Sbjct: 361 KELQINGYTIPEGWTVMLVTASRHRDPAVYKDPHTFNPWRWKELDSITIQKNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHILFTKYRW KLKGGK+ARAHIL FEDGL+V FTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILFTKYRWTKLKGGKVARAHILSFEDGLHVKFTPKE 474

BLAST of CsaV3_1G007770 vs. ExPASy TrEMBL
Match: A0A6J1K5M4 (cytochrome P450 87A3-like OS=Cucurbita maxima OX=3661 GN=LOC111490271 PE=3 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.3e-250
Identity = 422/474 (89.03%), Postives = 452/474 (95.36%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTI++GLATLA+AYYIHW+NKWKDSKFNGVLPPGTMGLPLIGET+QLSRPSDSLDVHPF
Sbjct: 1   MWTIVVGLATLAVAYYIHWINKWKDSKFNGVLPPGTMGLPLIGETLQLSRPSDSLDVHPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           I++KVKRYG IFKTCLAGRPVVVSTDAEFN+YIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IKKKVKRYGSIFKTCLAGRPVVVSTDAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           KALG IHKYIRSITLNHFGAESLRERFLPRIEESA+ETL YW+TQ SVEVK+SAA MVFR
Sbjct: 121 KALGFIHKYIRSITLNHFGAESLRERFLPRIEESAKETLCYWATQPSVEVKDSAAVMVFR 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
           TS+VKM S+DSSKLLT GLTKKFTGLLGGFLTLP+N+PGTTY+KC+KDMK+IQKKL++IL
Sbjct: 181 TSMVKMVSKDSSKLLTGGLTKKFTGLLGGFLTLPINVPGTTYNKCMKDMKEIQKKLREIL 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300
           E RLA G   DEDFLGQA+KDK SQ+FIS++FIIQLLFSISFASFESISTTLTLILN+LA
Sbjct: 241 EGRLASGAGSDEDFLGQAVKDKGSQKFISDDFIIQLLFSISFASFESISTTLTLILNYLA 300

Query: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360
           DHPDVVKELEAEHEAIR ARADPDGPITWEEYKSM FTL+VI ETLRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRNARADPDGPITWEEYKSMTFTLHVIFETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420
           KE+QI GYTIPEGWTVMLVTASRHRDP VYKDP TFNPWRWKELDSITIQKNFMPFGGGL
Sbjct: 361 KELQINGYTIPEGWTVMLVTASRHRDPAVYKDPHTFNPWRWKELDSITIQKNFMPFGGGL 420

Query: 421 RHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPKE 475
           RHCAGAEYSKVYLCTFLHILFTKYRW KLKGGK+ARAHIL FEDGL++ FTPKE
Sbjct: 421 RHCAGAEYSKVYLCTFLHILFTKYRWTKLKGGKVARAHILSFEDGLHMKFTPKE 474

BLAST of CsaV3_1G007770 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 419.5 bits (1077), Expect = 3.7e-117
Identity = 201/474 (42.41%), Postives = 299/474 (63.08%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MW +L+ ++ L I+   HWV  W++ K  G LPPG+MG PL+GE+IQ  +P+ + D+ PF
Sbjct: 1   MWALLIWVSLLLIS-ITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPF 60

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           I+ +VK+YGPIFKT L GRPV+VSTDA+ ++++  QEGR  + WY DT +  FG      
Sbjct: 61  IKERVKKYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVG- 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
              G ++KY++++ L  FG + L+ + LP++E +A + L  WS Q SVE+K++ A+M+F 
Sbjct: 121 SLHGFMYKYLKNMVLTLFGHDGLK-KMLPQVEMTANKRLELWSNQDSVELKDATASMIFD 180

Query: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240
            +  K+ S D  K  +E L   F   + G ++ P ++PGT YHKC++   +  K L+++L
Sbjct: 181 LTAKKLISHDPDK-SSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNML 240

Query: 241 EERLAKGVKIDEDFLGQAIKDKESQ-QFISEEFIIQLLFSISFASFESISTTLTLILNFL 300
           +ER     K   DF    I++ + +   ++EE  + L+F + FASFE+ S  LTL + FL
Sbjct: 241 QERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFL 300

Query: 301 ADHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKT 360
           +D P+V+K L  EHE I + R D D  +TWEEYKSM +T   I ET RL ++ PA+ RK 
Sbjct: 301 SDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKA 360

Query: 361 TKEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGG 420
            ++I+ K YTIP GW VM+   + H +PE+YKDP  FNP RW+        K+FM FGGG
Sbjct: 361 LRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGG 420

Query: 421 LRHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPK 474
           +R C G +++K+ +  FLH L TKYRW ++KGG I R   L+F +G +V    K
Sbjct: 421 MRFCVGTDFTKLQMAAFLHSLVTKYRWEEIKGGNITRTPGLQFPNGYHVKLHKK 470

BLAST of CsaV3_1G007770 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 414.1 bits (1063), Expect = 1.5e-115
Identity = 202/480 (42.08%), Postives = 299/480 (62.29%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MW +L+ ++ L I+   HWV  W++ K  G LPPG+MG PL+GE+IQ  +P+ + D+ PF
Sbjct: 1   MWALLIWVSLLLIS-ITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPF 60

Query: 61  IQRKVK------RYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFG 120
           I+ +VK      RYGPIFKT L GRPV+VSTDA+ ++++  QEGR  + WY DT +  FG
Sbjct: 61  IKERVKNDVDMCRYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFG 120

Query: 121 LDTEWLKALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESA 180
                    G ++KY++++ L  FG + L+ + LP++E +A + L  WS Q SVE+K++ 
Sbjct: 121 KKNVG-SLHGFMYKYLKNMVLTLFGHDGLK-KMLPQVEMTANKRLELWSNQDSVELKDAT 180

Query: 181 AAMVFRTSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQK 240
           A+M+F  +  K+ S D  K  +E L   F   + G ++ P ++PGT YHKC++   +  K
Sbjct: 181 ASMIFDLTAKKLISHDPDK-SSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMK 240

Query: 241 KLKDILEERLAKGVKIDEDFLGQAIKDKESQ-QFISEEFIIQLLFSISFASFESISTTLT 300
            L+++L+ER     K   DF    I++ + +   ++EE  + L+F + FASFE+ S  LT
Sbjct: 241 MLRNMLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALT 300

Query: 301 LILNFLADHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTP 360
           L + FL+D P+V+K L  EHE I + R D D  +TWEEYKSM +T   I ET RL ++ P
Sbjct: 301 LAIKFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVP 360

Query: 361 ALLRKTTKEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNF 420
           A+ RK  ++I+ K YTIP GW VM+   + H +PE+YKDP  FNP RW+        K+F
Sbjct: 361 AIFRKALRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHF 420

Query: 421 MPFGGGLRHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFTPK 474
           M FGGG+R C G +++K+ +  FLH L TKYRW ++KGG I R   L+F +G +V    K
Sbjct: 421 MAFGGGMRFCVGTDFTKLQMAAFLHSLVTKYRWEEIKGGNITRTPGLQFPNGYHVKLHKK 476

BLAST of CsaV3_1G007770 vs. TAIR 10
Match: AT3G44970.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 8.3e-85
Identity = 172/476 (36.13%), Postives = 276/476 (57.98%), Query Frame = 0

Query: 2   WTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPFI 61
           W     +  L +A   HW  +W + K NG LPPG+MG P+IGET+   +P    ++ P++
Sbjct: 5   WNTGFCVIVLVVARVGHWWYQWSNPKSNGKLPPGSMGFPIIGETLDFFKPYGFYEISPYL 64

Query: 62  QRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWLK 121
           ++K+ RYGP+F+T + G   VVSTD + N  I+ QE ++  + Y D L K  G D+ +LK
Sbjct: 65  KKKMLRYGPLFRTNILGVKTVVSTDKDVNMEILRQENKSFILSYPDGLMKPLGKDSLFLK 124

Query: 122 ALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFRT 181
            +G IHK+I+ ITL+   +E L+ + L  ++   RE L   +    ++VK++ + ++   
Sbjct: 125 -IGNIHKHIKQITLHLLSSEGLKRKILKDMDRVTREHLSSKAKTGRLDVKDAVSKLIIAH 184

Query: 182 SIVKMFS----EDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLK 241
              KM S    +  +KL+  G+ K FT     F T  L   G   +  +   ++  +++K
Sbjct: 185 LTPKMMSNLKPQTQAKLM--GIFKAFT--FDWFRTSYLISAGKGLYNTLWACREGMREIK 244

Query: 242 DILEERLAKGVKIDEDFLGQAIKDKE-SQQFISEEFIIQLLFSISFASFESISTTLTLIL 301
           DI   R     K D DFL  AI++ E + + ++E  II L+F++S  + ++ S  + L +
Sbjct: 245 DIYTMRKTSEEKYD-DFLNTAIEESEKAGELLNENAIITLIFTLSCVTQDTTSKAICLAV 304

Query: 302 NFLADHPDVVKELEAEHEAIRKARADPDGPITWEEYK-SMNFTLNVICETLRLGSVTPAL 361
            FL ++P V+ EL+ EHE I ++R D +G +TWEEY+  M FT  VI E+LR+ ++ P L
Sbjct: 305 KFLLENPKVLAELKKEHEVILESREDKEGGVTWEEYRHKMTFTNMVINESLRITNLAPML 364

Query: 362 LRKTTKEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMP 421
            RK  K+++IKGYTIP GW VM++ +  H DPE+Y++P  FNPWRW+  +     K FM 
Sbjct: 365 FRKAVKDVEIKGYTIPAGWIVMIIPSVVHFDPEIYENPFEFNPWRWEGKELRAGSKTFMV 424

Query: 422 FGGGLRHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFT 472
           FG GLR CAGAE++++ +  FLH L T Y +   +  ++ R       +G+ +N +
Sbjct: 425 FGTGLRQCAGAEFARLQISVFLHHLVTTYNFSLHQDCEVLRVPAAHLPNGISINIS 474

BLAST of CsaV3_1G007770 vs. TAIR 10
Match: AT1G55940.1 (cytochrome P450, family 708, subfamily A, polypeptide 1 )

HSP 1 Score: 311.6 bits (797), Expect = 1.1e-84
Identity = 176/496 (35.48%), Postives = 277/496 (55.85%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIH-WVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHP 60
           MW + L      +   I  W+ +W +   +G LPPG+MG P+IGET++  +P    ++HP
Sbjct: 148 MWGVALSFVIALVVVKISLWLYRWANPNCSGKLPPGSMGFPVIGETVEFFKPYSFNEIHP 207

Query: 61  FIQRKV-KRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTE 120
           F+++++ K  G +F+T + G   +VSTD E N  I+ QE R   M Y + L + FG D  
Sbjct: 208 FVKKRMFKHGGSLFRTNILGSKTIVSTDPEVNFEILKQENRCFIMSYPEALVRIFGKDNL 267

Query: 121 WLKALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMV 180
           + K     H+Y+R I L   G E L++RF+ +I+ +  E L   S Q  V+VK+++  ++
Sbjct: 268 FFKQGKDFHRYMRHIALQLLGPECLKQRFIQQIDIATSEHLKSVSFQGVVDVKDTSGRLI 327

Query: 181 FRTSIVKMFS----EDSSKLLTEGLTKKF------------TGLLGGFLTLP--LNLPGT 240
               I+ + S    E  SKL+       F              L  G +     LN+   
Sbjct: 328 LEQMILMIISNIKPETKSKLIESFRDFSFDLVRSPFDPSFWNALYNGLMDFVWLLNILDP 387

Query: 241 TYHKCIKDMKQIQKKLKDILEERLAKGVKIDE---DFLGQAIKDKESQ-QFISEEFIIQL 300
            ++  +K    + K LK + +ER  +    D    DF+   I + E +   I+EE  ++L
Sbjct: 388 LFY--VKARSNVMKMLKRMFKERREEATSDDSKYGDFMETMIYEVEKEGDTINEERSVEL 447

Query: 301 LFSISFASFESISTTLTLILNFLADHPDVVKELEAEHEAIRKARADPDGPITWEEYKS-M 360
           + S+  AS+E+ ST   L + F+A++P V+ EL+ EHE I + RAD +  +TW+EY+S M
Sbjct: 448 ILSLLIASYETTSTMTALTVKFIAENPKVLMELKREHETILQNRADKESGVTWKEYRSMM 507

Query: 361 NFTLNVICETLRLGSVTPALLRKTTKEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDT 420
           NFT  VI E+LRLGS++PA+ RK   +++IKGYTIP GW V++V +  H DP++Y+ P  
Sbjct: 508 NFTHMVINESLRLGSLSPAMFRKAVNDVEIKGYTIPAGWIVLVVPSLLHYDPQIYEQPCE 567

Query: 421 FNPWRWKELDSITIQKNFMPFGGGLRHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIA 472
           FNPWRW+  + ++  K FM FGGG R CAGAE++++ +  FLH L T Y +  +    I 
Sbjct: 568 FNPWRWEGKELLSGSKTFMAFGGGARLCAGAEFARLQMAIFLHHLVTTYDFSLIDKSYII 627

BLAST of CsaV3_1G007770 vs. TAIR 10
Match: AT1G78490.1 (cytochrome P450, family 708, subfamily A, polypeptide 3 )

HSP 1 Score: 290.4 bits (742), Expect = 2.6e-78
Identity = 163/477 (34.17%), Postives = 261/477 (54.72%), Query Frame = 0

Query: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           +W + + +  L +    HW+ +W + K  G LPPG+MG P+IGET+   +P     +  F
Sbjct: 4   IWNVAMLMVALVVVRISHWLYRWSNPKCPGKLPPGSMGFPIIGETLDFFKPCGVEGIPTF 63

Query: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           +++++ RYGP+F+T + G   VVSTD +  H I  QE  + E+ Y D   K FG D  +L
Sbjct: 64  VKKRMIRYGPLFRTNIFGSKTVVSTDPDVIHQIFRQENTSFELGYPDIFVKVFGKDNLFL 123

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180
           K +  IHKY++ IT+   G+E L++  L  ++++ R+ +   ++Q S  V++    +V  
Sbjct: 124 KEV-FIHKYLQKITMQILGSEGLKQTMLGNMDKATRDHIRSIASQGSFNVRKEVENLVVA 183

Query: 181 TSIVKMFS----EDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKL 240
               K+ S    E  SKL+ + L          FL L      T   K +K  ++  + +
Sbjct: 184 YMTPKLISNLKPETQSKLI-DNLNAFNLDWFKSFLRLSTWKAVT---KALKSREEAIQVM 243

Query: 241 KDILEERLAKGVKIDEDFLGQAIKDKESQ-QFISEEFIIQLLFSISFASFESISTTLTLI 300
           KD+L  R     K  EDFL   +++ E    F  +   I L+F ++FA  E  S+   L 
Sbjct: 244 KDVLMMRKETREK-QEDFLNTLLEELEKDGSFFDQGSAINLIFLLAFALREGTSSCTALA 303

Query: 301 LNFLADHPDVVKELEAEHEAIRKARADPDGPITWEEYK-SMNFTLNVICETLRLGSVTPA 360
           + F++  P V+ EL+ EH+AI   R D +  ++WEEY+ +M FT  V  E LRL + TP 
Sbjct: 304 VKFISKDPKVLAELKREHKAIVDNRKDKEAGVSWEEYRHNMTFTNMVSNEVLRLANTTPL 363

Query: 361 LLRKTTKEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFM 420
           L RK  ++++IKGYTIP GW V +  ++ H DP +Y++P  FNPWRW+  + I   K FM
Sbjct: 364 LFRKAVQDVEIKGYTIPAGWIVAVAPSAVHFDPAIYENPFEFNPWRWEGKEMIWGSKTFM 423

Query: 421 PFGGGLRHCAGAEYSKVYLCTFLHILFTKYRWRKLKGGKIARAHILRFEDGLYVNFT 472
            FG G+R C GAE+S++ +  FLH L   Y +  ++  +I R+   ++   L +N +
Sbjct: 424 AFGYGVRLCVGAEFSRLQMAIFLHHLVAYYDFSMVQDSEIIRSPFHQYTKDLLINIS 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NP_001289636.11.1e-277100.00Cytochrome P450 87A3-like [Cucumis sativus] >AIT72037.1 cytochrome P450 [Cucumis... [more]
XP_008437370.11.7e-27398.31PREDICTED: cytochrome P450 87A3-like [Cucumis melo] >TYJ99092.1 cytochrome P450 ... [more]
XP_038895732.17.1e-26494.73cucurbitadienol 11-hydroxylase [Benincasa hispida][more]
KAG6573008.18.2e-25289.24Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7012193.11.1e-25189.24Cytochrome P450 87A3 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
K7NBR25.0e-23683.33Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=... [more]
Q7XU384.4e-11542.04Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV... [more]
Q431471.7e-7935.49Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1[more]
Q69F953.4e-7534.89Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2[more]
Q50LE01.4e-7333.41Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LQP65.5e-278100.00Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa1G044890 PE=2 SV=1[more]
A0A5D3BH608.2e-27498.31Cytochrome P450 87A3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3AUF58.2e-27498.31cytochrome P450 87A3-like OS=Cucumis melo OX=3656 GN=LOC103482802 PE=3 SV=1[more]
A0A6J1GV283.3e-25189.03cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111457186 PE=3 SV=... [more]
A0A6J1K5M41.3e-25089.03cytochrome P450 87A3-like OS=Cucurbita maxima OX=3661 GN=LOC111490271 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G12740.13.7e-11742.41cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
AT1G12740.21.5e-11542.08cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
AT3G44970.18.3e-8536.13Cytochrome P450 superfamily protein [more]
AT1G55940.11.1e-8435.48cytochrome P450, family 708, subfamily A, polypeptide 1 [more]
AT1G78490.12.6e-7834.17cytochrome P450, family 708, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 222..242
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 2..444
e-value: 7.0E-17
score: 59.3
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 9..453
NoneNo IPR availablePANTHERPTHR24286:SF90CYTOCHROME P450 87A3-LIKEcoord: 9..453
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 384..402
score: 39.27
coord: 423..441
score: 21.86
coord: 407..423
score: 32.13
coord: 59..82
score: 27.4
coord: 368..382
score: 23.85
coord: 335..351
score: 38.01
IPR001128Cytochrome P450PFAMPF00067p450coord: 33..453
e-value: 9.0E-56
score: 189.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..471
e-value: 2.0E-93
score: 315.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 32..472
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 416..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G007770.1CsaV3_1G007770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen