CsaV3_1G003090 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_1G003090
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionPhosphopyruvate hydratase
Locationchr1: 1932281 .. 1935918 (+)
RNA-Seq ExpressionCsaV3_1G003090
SyntenyCsaV3_1G003090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CGACTAACTATAGGTGAATCAAATTAAATTGATTTTTCTGTCTATAATGTTAACATGGAGTTATTTGATTAGCCTTACCTAACCCTTCAGGGCCAAAATTCGATTGCCAAGTGAGTCGCTTTCAACATTCTCGTCGCCATTTCCCAACAACCACGCTAACCCCTGCTGGCTGCTGCTCTGCTTTGAACGCCCCTTTCCTTTGGCGGACTTTCCCACTTTCTTCTTCTTCTTCTACATATACATTCACTCACTTCTCCATTTTCATTTCTTCTCATCAGAACTTCAATGGCTTCTCTTCCTTCCCCACACACCATGAATCTCCTCAACAAATCCCTCTCTCTTTCCCCACCTCAATCCTTCATTGCCTTAATACCTTCTTCTTCTTCTTCTTCTTCCTCCAAACCCCTTCGTTCCCCTACTATTCGCTGTTCTGTTGCTGTGGCTCCTTCTGTTTCAACCAACGTGTCCAAGGAATTCAAGTTGAAGTCCCTCAAGGCCCGTCAGATCATTGACAGTAGAGGCAATCCTACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTTCCTAGTGGGGCTTCTACTGGGATCTATGAGGCGTTGGAGCTTAGAGATGGGGACAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAATATCAATGATATCTTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGTTGTTGATTCGATTCTTTCTTTATTTGCTTTTATGAGCATTAATTTGAATGTTTGTTGGATTGTAATACTGACTTCTAATTGTGATCATTGGAATTTGGATTTTAGTTCGTTTGATTATTGACTGTAAATCTGCTTTTGTAATTAAGTAGACCTTTTATAATCTATGTCCACGAGTCTAGACTTTAAGTTGCAGAAAGAGACTTGTTTTCGAAAATTGACGTTGGTTTTGAATGAATTTCTAGGTGCTTAAAAACATTTTTTTTTCGCTTAAAACTACTTCTCCAAATATTAGAAACTTATTCCAAAAACACCCTTCAAGTACTTATCTTGATTGTGAGTCTTGTGTGTAAAAGCCAAAAGGTGTCTTAGTTCAGATTGAGTTTTCAACCTAATTGTTGCGAGGCAACGACGTGAATGGAACAGAATAATGGTGGAAATTTTCGTTATTGGGGATTCTCAAACTATATGCTTTTTTTTTTTTTTTTTTTTTGCTGTACTTACTGGTCGGAAAAAGTTGGTAGCCTTTCTATTCAATTTATTACTGAAGATGTAAAATTCCAAGCCTTTTCATTGGTTTTGAAAGAGTAAAGGTATTCAAAATAACATGCCAACATAAAGTCTTGCCTAAAGAAAGAGTTTGTCGTTGGTCTGTCTTTGTGATAGTTGTTTAAGAATAGAATCAATTTTCATTGATGAATGAAATAAATAGTCAAACTCCCAAACACCTTAGTCTCTGTGTTACAGGATAAGCTTCCAGTTATTGATCAAAGAAAAAAGACTAGAATGAGAAGAAAAGAGGGGGAAAAAAGTGTCTTTGTGGGAGTTGGTTCACTTTTCAGTACTTCTGCTTCCTAACCTAAGTTTTTCAACTTCTGTAGGAATCAAGAAGAAGTTGATGCAATCATGCTGGAAATTGATGGAACTCCAAACAAGTCGGAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGTGTCTGCAGAGCTGGTGCTGGCGCTAAGGGACTACCATTGTATAGACACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAACGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGTGCTTCATCTTTCGCAGAGGCACTTCGGATGGGAAGTGAAGGTTGGCAGCTGCTTCTCAAGTGCTAAAATTTTCTTTTCCATTGTTATGGTGTTAGTTTATGATTCATTTTGCACCTCATCTTCGTCATTTTGTACTTAAACAAGTTATTTATGAGTAGCCTGACTTTGGTATTTTCTAATGAACAGTTTATCACACACTAAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGCTATACAGGAAAGGTGCAGGATTGTAAAATTTCCTTATTGTTTTGAAATGGTGTAGAAGTTTTCTTACATGTCCTGCTAAGACCAAAAAAAATAAATGAAAATTTACTTTGATACAGATCAAAATAGGAATGGATGTTGCAGCTTCCGAGTTTTTCACTGCAGAGGGAAAATATGATTTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTCCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGTTCCTGGGCTTCTCTACAATCTTCCGTTGATATCCAACTTGTGGGAGATGATTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGGCATTCAGAAAAAAGCTTGTAATGCTTTACTTCTGAAGGTAAGCAGCCTCTGATTTTGTTCTTTCCTTTTTATCTCTGTTCTAGACTAATTGTAGGATTTAGGTTGGTCCTTAAAAATTTTACTTGTTTGCTGAATGTTTGGTTTTCCATTGATTTAGGTTGGTCCTTAGAAACATGTGATTTTGATGCTGCAGCTTGCTCATATGTTAGAAACTTCTCCTGCAGGTTAACCAAATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCGGATCTTTCAGTTGGCTTGGCTAGTGGACAGGTTTCATTCATCTTCAGCTTTTCTGTCTACAACTATAAGTCCATAACGCATGTATGTTGTTGACATAATTCACAACTGAATATTGTGTTACTATTCTACAGATCAAAACTGGAGCTCCATGCCGAAGCGAACGGTTAGCCAAATATAATCAGGTAAAGTTTGACCAAATCTGGACATCCAAAATTATTGTCCTTGTATTGCTAGAATATTTCTAGTTTTGCTCAATGTATTTATATATTCTTTTTCTTTTTCTTTTTTAATTTTGATGCAGCTTCTTCGCATTGAAGAAGAACTCGGCAGTGTTCGATATGCCGGTGCGTCTTTCAGATCACCTTAGGAGTTAAAAGTTCAAGGTGATGACTGTGTTGATGTATCAACTTTAGGCTTGATAGAAGAACATGTAGAGGATTTCTTCAGAAATAATATGCTCAACTTTTCTGTCCAAGGTGTTAGAATCTGACACGTTACGAAGTTCATTCGAGTGTTGCCATGAGAGTATTTAAATTTTTGTTATGAACTATTCGTTTCAAAATAATGCAGTGTTTTGATTCACAAACTACCTCGTGGGGTTCTCTTTTCTTCAAAGCTATTCCTTACAGCCAAATATTAGAAGTCTGATTAGTTCATCTAAAGTATGTCATCAACCATTGATAAACAAATAATAGTAACCAAGC

mRNA sequence

ATGGCTTCTCTTCCTTCCCCACACACCATGAATCTCCTCAACAAATCCCTCTCTCTTTCCCCACCTCAATCCTTCATTGCCTTAATACCTTCTTCTTCTTCTTCTTCTTCCTCCAAACCCCTTCGTTCCCCTACTATTCGCTGTTCTGTTGCTGTGGCTCCTTCTGTTTCAACCAACGTGTCCAAGGAATTCAAGTTGAAGTCCCTCAAGGCCCGTCAGATCATTGACAGTAGAGGCAATCCTACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTTCCTAGTGGGGCTTCTACTGGGATCTATGAGGCGTTGGAGCTTAGAGATGGGGACAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAATATCAATGATATCTTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGAATCAAGAAGAAGTTGATGCAATCATGCTGGAAATTGATGGAACTCCAAACAAGTCGGAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGTGTCTGCAGAGCTGGTGCTGGCGCTAAGGGACTACCATTGTATAGACACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAACGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGTGCTTCATCTTTCGCAGAGGCACTTCGGATGGGAAGTGAAGTTTATCACACACTAAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGCTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCCGAGTTTTTCACTGCAGAGGGAAAATATGATTTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTCCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGTTCCTGGGCTTCTCTACAATCTTCCGTTGATATCCAACTTGTGGGAGATGATTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGGCATTCAGAAAAAAGCTTGTAATGCTTTACTTCTGAAGGTTAACCAAATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCGGATCTTTCAGTTGGCTTGGCTAGTGGACAGATCAAAACTGGAGCTCCATGCCGAAGCGAACGGTTAGCCAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTCGGCAGTGTTCGATATGCCGGTGCGTCTTTCAGATCACCTTAG

Coding sequence (CDS)

ATGGCTTCTCTTCCTTCCCCACACACCATGAATCTCCTCAACAAATCCCTCTCTCTTTCCCCACCTCAATCCTTCATTGCCTTAATACCTTCTTCTTCTTCTTCTTCTTCCTCCAAACCCCTTCGTTCCCCTACTATTCGCTGTTCTGTTGCTGTGGCTCCTTCTGTTTCAACCAACGTGTCCAAGGAATTCAAGTTGAAGTCCCTCAAGGCCCGTCAGATCATTGACAGTAGAGGCAATCCTACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTTCCTAGTGGGGCTTCTACTGGGATCTATGAGGCGTTGGAGCTTAGAGATGGGGACAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAATATCAATGATATCTTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGAATCAAGAAGAAGTTGATGCAATCATGCTGGAAATTGATGGAACTCCAAACAAGTCGGAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGTGTCTGCAGAGCTGGTGCTGGCGCTAAGGGACTACCATTGTATAGACACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAACGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGTGCTTCATCTTTCGCAGAGGCACTTCGGATGGGAAGTGAAGTTTATCACACACTAAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGCTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCCGAGTTTTTCACTGCAGAGGGAAAATATGATTTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTCCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGTTCCTGGGCTTCTCTACAATCTTCCGTTGATATCCAACTTGTGGGAGATGATTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGGCATTCAGAAAAAAGCTTGTAATGCTTTACTTCTGAAGGTTAACCAAATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCGGATCTTTCAGTTGGCTTGGCTAGTGGACAGATCAAAACTGGAGCTCCATGCCGAAGCGAACGGTTAGCCAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTCGGCAGTGTTCGATATGCCGGTGCGTCTTTCAGATCACCTTAG

Protein sequence

MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP*
Homology
BLAST of CsaV3_1G003090 vs. NCBI nr
Match: XP_004138202.1 (enolase 1, chloroplastic [Cucumis sativus] >KGN63743.1 hypothetical protein Csa_014309 [Cucumis sativus])

HSP 1 Score: 959.1 bits (2478), Expect = 1.4e-275
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60
           MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV
Sbjct: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60

Query: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120
           SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG
Sbjct: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120

Query: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180
           GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR
Sbjct: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180

Query: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240
           AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE
Sbjct: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240

Query: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300
           ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI
Sbjct: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300

Query: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360
           KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ
Sbjct: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360

Query: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420
           DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL
Sbjct: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420

Query: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480
           DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG
Sbjct: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480

Query: 481 SVRYAGASFRSP 493
           SVRYAGASFRSP
Sbjct: 481 SVRYAGASFRSP 492

BLAST of CsaV3_1G003090 vs. NCBI nr
Match: XP_008453338.1 (PREDICTED: enolase 1, chloroplastic [Cucumis melo] >KAA0058034.1 enolase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 935.6 bits (2417), Expect = 1.7e-268
Identity = 484/496 (97.58%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIP----SSSSSSSSKPLRSPTIRCSVAVAPSV 60
           MASLPSP TMNLLNK LSLSPPQSFI  IP    SSSSSSSSKPLRSPTIRCSVAVAPSV
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSL 180
           TVYGGKGVLTAVKNI DILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFT EGKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 493
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of CsaV3_1G003090 vs. NCBI nr
Match: TYK28380.1 (enolase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 935.3 bits (2416), Expect = 2.2e-268
Identity = 484/496 (97.58%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIP----SSSSSSSSKPLRSPTIRCSVAVAPSV 60
           MASLPSP TMNLLNK LSLSPPQSFI  IP    SSSSSSSSKPLRSPTIRCSVAVAPSV
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSL 180
           TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFT  GKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 493
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of CsaV3_1G003090 vs. NCBI nr
Match: XP_038879680.1 (enolase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 914.1 bits (2361), Expect = 5.3e-262
Identity = 471/492 (95.73%), Postives = 478/492 (97.15%), Query Frame = 0

Query: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60
           MASLPSP TMNLL KSL  S PQSFI+ IP  SSSSSSKPLRS  IRCSVAVAPSVST  
Sbjct: 1   MASLPSPPTMNLLEKSLLPSSPQSFISSIP--SSSSSSKPLRSHAIRCSVAVAPSVSTKA 60

Query: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120
           SKEFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG
Sbjct: 61  SKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120

Query: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180
           GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSLSVCR
Sbjct: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCR 180

Query: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240
           AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA SFAE
Sbjct: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAISFAE 240

Query: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300
           ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDN+EGLVLLIDAIEKAGYTGKI
Sbjct: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNKEGLVLLIDAIEKAGYTGKI 300

Query: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360
           KIGMDVAASEFFT EGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ
Sbjct: 301 KIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360

Query: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420
           DDWSSWASLQSSVDIQLVGDDLLVTNPKRIA+GIQKKACNALLLKVNQIGSVTESIQAAL
Sbjct: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAKGIQKKACNALLLKVNQIGSVTESIQAAL 420

Query: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480
           DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE+G
Sbjct: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEIG 480

Query: 481 SVRYAGASFRSP 493
           S+RYAGASFRSP
Sbjct: 481 SIRYAGASFRSP 490

BLAST of CsaV3_1G003090 vs. NCBI nr
Match: KAG6587623.1 (Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 879.8 bits (2272), Expect = 1.1e-251
Identity = 456/494 (92.31%), Postives = 467/494 (94.53%), Query Frame = 0

Query: 1   MASLPSP--HTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVST 60
           MASLPSP   T NLLNKSL  S PQS I  IP   SSSSSKPLRS  +RCSVAVAPS ST
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIP---SSSSSKPLRSHGVRCSVAVAPSAST 60

Query: 61  NVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTV 120
             S+EFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK V
Sbjct: 61  KASREFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNV 120

Query: 121 YGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSV 180
           YGGKGVLTAVKNINDILAPKLVGVDVRNQE+VDAIMLEIDGT NKS+LGANAILGVSLSV
Sbjct: 121 YGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSV 180

Query: 181 CRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSF 240
           CRAGAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+SF
Sbjct: 181 CRAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASF 240

Query: 241 AEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTG 300
           AEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTG
Sbjct: 241 AEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTG 300

Query: 301 KIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPF 360
           KIKIGMDVAASEFFT +G+YDLNFKKQPNDGAHVHSAHGLGELYKQFV DFPIVSIEDPF
Sbjct: 301 KIKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPF 360

Query: 361 DQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQA 420
           DQDDWSSWASLQSSV +Q+VGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESIQA
Sbjct: 361 DQDDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQA 420

Query: 421 ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE 480
           ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE
Sbjct: 421 ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE 480

Query: 481 LGSVRYAGASFRSP 493
           LG+VRYAG SFRSP
Sbjct: 481 LGNVRYAGESFRSP 491

BLAST of CsaV3_1G003090 vs. ExPASy Swiss-Prot
Match: Q9C9C4 (Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 1.8e-225
Identity = 391/459 (85.19%), Postives = 427/459 (93.03%), Query Frame = 0

Query: 34  SSSSSKPLRSPTIRCSVAVAPSVSTNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLY 93
           S+ S    R   ++CSV         V+KE ++K +KARQIIDSRGNPTVEVDLITDDLY
Sbjct: 28  SAPSCLRFRRSGVQCSV---------VAKECRVKGVKARQIIDSRGNPTVEVDLITDDLY 87

Query: 94  RSAVPSGASTGIYEALELRDGDKTVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAI 153
           RSAVPSGASTGIYEALELRDGDK+VYGGKGVL A+KNIN+++APKL+GVDVRNQ +VDA+
Sbjct: 88  RSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAIKNINELVAPKLIGVDVRNQADVDAL 147

Query: 154 MLEIDGTPNKSELGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 213
           MLE+DGTPNKS+LGANAILGVSLSVCRAGAGAKG+PLY+HIQE SGTKELVMPVPAFNVI
Sbjct: 148 MLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVPLYKHIQETSGTKELVMPVPAFNVI 207

Query: 214 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 273
           NGGSHAGN+LAMQEFMILPVGA+SF+EA +MGSEVYHTLKGIIK KYGQDACNVGDEGGF
Sbjct: 208 NGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGF 267

Query: 274 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVH 333
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFF  +G+YDLNFKKQPNDGAHV 
Sbjct: 268 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVL 327

Query: 334 SAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 393
           SA  L +LY++F+KDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE 
Sbjct: 328 SAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEA 387

Query: 394 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 453
           I+K++CNALLLKVNQIG+VTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 388 IKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 447

Query: 454 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 493
           QIKTGAPCRSERL+KYNQLLRIEEELG+VRYAG +FRSP
Sbjct: 448 QIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEAFRSP 477

BLAST of CsaV3_1G003090 vs. ExPASy Swiss-Prot
Match: Q54RK5 (Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 1.7e-170
Identity = 302/431 (70.07%), Postives = 358/431 (83.06%), Query Frame = 0

Query: 66  LKSLKARQIIDSRGNPTVEVDLITD-----DLYRSAVPSGASTGIYEALELRDGDKTVYG 125
           +KS+KAR+I++SRGNPTVEVDL T+       +R+AVPSGASTGIYEA+ELRDGDK+ Y 
Sbjct: 4   IKSIKAREILNSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVELRDGDKSRYL 63

Query: 126 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 185
           GKGVL A+KNI +++ P ++G  V +Q  +D +M+++DGTPNK +LGANAIL VSL+VCR
Sbjct: 64  GKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANAILAVSLAVCR 123

Query: 186 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 245
           AGA  + LPLY++I E++GTK + +PVPAFNVINGGSHAGN LAMQEFMILPVGA  F E
Sbjct: 124 AGAADRNLPLYKYISEIAGTK-MRLPVPAFNVINGGSHAGNKLAMQEFMILPVGAKDFNE 183

Query: 246 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 305
           A RMGSEVYH LK +I  +YGQDA NVGDEGGFAP +Q N+EGL LL  AIEKAGYTG +
Sbjct: 184 AYRMGSEVYHNLKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLV 243

Query: 306 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 365
           KIGMD AASE F  E  YDL+FK + NDG+ V S   LG+LY++F+K++PI+SIEDPFDQ
Sbjct: 244 KIGMDCAASE-FKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQ 303

Query: 366 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 425
           DDW S+  L +SVDIQ+VGDDLLVTNP+RI  GI+KKACNALLLKVNQIGSVTESI+AAL
Sbjct: 304 DDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAAL 363

Query: 426 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 485
           DSK A WGVMVSHRSGETED FIADL VGL +GQIKTGAPCRSERLAKYNQL+RI EELG
Sbjct: 364 DSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELG 423

Query: 486 -SVRYAGASFR 491
            +  YAG +FR
Sbjct: 424 ENHNYAGLTFR 432

BLAST of CsaV3_1G003090 vs. ExPASy Swiss-Prot
Match: P15429 (Beta-enolase OS=Rattus norvegicus OX=10116 GN=Eno3 PE=1 SV=3)

HSP 1 Score: 591.7 bits (1524), Expect = 7.9e-168
Identity = 302/431 (70.07%), Postives = 355/431 (82.37%), Query Frame = 0

Query: 66  LKSLKARQIIDSRGNPTVEVDLIT-DDLYRSAVPSGASTGIYEALELRDGDKTVYGGKGV 125
           ++ + AR+I+DSRGNPTVEVDL T    +R+AVPSGASTGIYEALELRDGDK+ Y GKGV
Sbjct: 3   MQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKSRYLGKGV 62

Query: 126 LTAVKNINDILAPKLV--GVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCRAG 185
           L AV++IN  L P L+   + V +QE+VD  M+E+DGT NKS+ GANAILGVSL+VC+AG
Sbjct: 63  LKAVEHINKTLGPALLEKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAG 122

Query: 186 AGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEAL 245
           A  KG+PLYRHI +L+G  +LV+PVPAFNVINGGSHAGN LAMQEFMILPVGASSF EA+
Sbjct: 123 AAEKGVPLYRHIADLAGNPDLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAM 182

Query: 246 RMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKI 305
           R+G+EVYH LKG+IKAKYG+DA NVGDEGGFAPN+ +N E L LL  AI+ AGY  K+ I
Sbjct: 183 RIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVI 242

Query: 306 GMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDD 365
           GMDVAASEF+   GKYDL+F K P+D A   S   LGELYK F+K++P+VSIEDPFDQDD
Sbjct: 243 GMDVAASEFY-RNGKYDLDF-KSPDDPARHISGEKLGELYKSFIKNYPVVSIEDPFDQDD 302

Query: 366 WSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAALDS 425
           W++W S  S VDIQ+VGDDL VTNPKRIA+ ++KKACN LLLKVNQIGSVTESIQA   +
Sbjct: 303 WATWTSFLSGVDIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLA 362

Query: 426 KAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG-S 485
           ++ GWGVMVSHRSGETED FIADL VGL +GQIKTGAPCRSERLAKYNQL+RIEE LG  
Sbjct: 363 QSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK 422

Query: 486 VRYAGASFRSP 493
             +AG  FR+P
Sbjct: 423 AVFAGRKFRNP 431

BLAST of CsaV3_1G003090 vs. ExPASy Swiss-Prot
Match: Q27527 (Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3)

HSP 1 Score: 591.3 bits (1523), Expect = 1.0e-167
Identity = 303/431 (70.30%), Postives = 352/431 (81.67%), Query Frame = 0

Query: 66  LKSLKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKTVYGGKGV 125
           +  + ARQI DSRGNPTVEVDL T+  ++R+AVPSGASTG++EALELRDGDK V+ GKGV
Sbjct: 3   ITKIHARQIYDSRGNPTVEVDLFTEKGVFRAAVPSGASTGVHEALELRDGDKAVHLGKGV 62

Query: 126 LTAVKNINDILAPKLV--GVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCRAG 185
           L AV NIN+ +AP L+  G DV  Q+++D  M+ +DG+ NK  LGANAILGVSL+V +AG
Sbjct: 63  LKAVSNINEKIAPALIAKGFDVTAQKDIDDFMMALDGSENKGNLGANAILGVSLAVAKAG 122

Query: 186 AGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEAL 245
           A  KGLPLY++I EL+GT ++V+PVPAFNVINGGSHAGN LAMQEFMILPVGASSFAEA+
Sbjct: 123 AVHKGLPLYKYIAELAGTGKVVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFAEAM 182

Query: 246 RMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKI 305
           RMGSEVYH LK  IK +YG DA  VGDEGGFAPN+QDN+EGL LL  AI+KAGYTGKI I
Sbjct: 183 RMGSEVYHHLKAEIKKRYGLDATAVGDEGGFAPNIQDNKEGLDLLNTAIDKAGYTGKISI 242

Query: 306 GMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDD 365
           GMDVAASEFF  +GKYDL+FK   +D +   S   L ELY+ F+K++P+VSIED FDQDD
Sbjct: 243 GMDVAASEFF-KDGKYDLDFKNPASDSSKWLSGEQLTELYQSFIKEYPVVSIEDAFDQDD 302

Query: 366 WSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAALDS 425
           W +W     +  IQLVGDDL VTNPKRI   I KK+CN LLLKVNQIGSVTESI+AA  S
Sbjct: 303 WDNWGKFHGATSIQLVGDDLTVTNPKRIQTAIDKKSCNCLLLKVNQIGSVTESIEAAKLS 362

Query: 426 KAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGS- 485
           +A GWGVMVSHRSGETED FIADL VGLA+GQIKTGAPCRSERLAKYNQLLRIEEELG+ 
Sbjct: 363 RANGWGVMVSHRSGETEDTFIADLVVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGAD 422

Query: 486 VRYAGASFRSP 493
             YAG +FR+P
Sbjct: 423 AVYAGHNFRNP 432

BLAST of CsaV3_1G003090 vs. ExPASy Swiss-Prot
Match: P42894 (Enolase OS=Neocallimastix frontalis OX=4757 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 1.0e-167
Identity = 301/434 (69.35%), Postives = 357/434 (82.26%), Query Frame = 0

Query: 66  LKSLKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKTVYGGKGV 125
           +  + ARQI DSRGNPTVEV++ TD  L+R+AVPSGASTG++EALELRDG K  Y GKGV
Sbjct: 3   ITKVHARQIFDSRGNPTVEVEVTTDKGLFRAAVPSGASTGVHEALELRDGIKADYVGKGV 62

Query: 126 LTAVKNINDILAPKLV--GVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCRAG 185
           L AV+N+N  +AP LV   +DV+NQ+ VD  +L++DGTPNKS+LGANAILGVSL+V RAG
Sbjct: 63  LKAVENVNKTIAPALVAANLDVKNQKAVDDFLLKLDGTPNKSKLGANAILGVSLAVARAG 122

Query: 186 AGAKGLPLYRHIQELSGTK-ELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEA 245
           A  KG+PLY+H+ EL+G K   ++PVP+ NV+NGGSHAGN LAMQEFMILP GA SF EA
Sbjct: 123 AADKGVPLYQHLGELAGNKGPWILPVPSMNVLNGGSHAGNKLAMQEFMILPTGAKSFTEA 182

Query: 246 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 305
           L+MGSEVYH LK +IKAKYGQDACNVGDEGGFAPN+QDN+EGL LL +AI KAGYTGK+K
Sbjct: 183 LKMGSEVYHALKSVIKAKYGQDACNVGDEGGFAPNIQDNKEGLELLNEAIAKAGYTGKVK 242

Query: 306 IGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 365
           IGMDVA+SEF+  +GKYDL+FK   +D +   S   LG+ YK+   ++PIVSIEDP+DQD
Sbjct: 243 IGMDVASSEFY-KDGKYDLDFKNPNSDPSKWISGEELGQFYKEITSEYPIVSIEDPYDQD 302

Query: 366 DWSSWASLQSSVD--IQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAA 425
           D+ SW+  ++ +   IQ+VGDDL VTNPKRIA  I+KKACN LLLKVNQIG+V+ESIQAA
Sbjct: 303 DFESWSKFRADMQDKIQIVGDDLTVTNPKRIAMAIEKKACNGLLLKVNQIGTVSESIQAA 362

Query: 426 LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEL 485
           LD+   GWGVMVSHRSGETED FIADL VGL SGQIKTGAPCRSERLAKYNQLLRIEEEL
Sbjct: 363 LDAFNDGWGVMVSHRSGETEDTFIADLVVGLKSGQIKTGAPCRSERLAKYNQLLRIEEEL 422

Query: 486 G-SVRYAGASFRSP 493
           G +  YAG +FR P
Sbjct: 423 GANATYAGENFRRP 435

BLAST of CsaV3_1G003090 vs. ExPASy TrEMBL
Match: A0A0A0LPK6 (Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G014400 PE=3 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 6.9e-276
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60
           MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV
Sbjct: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60

Query: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120
           SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG
Sbjct: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120

Query: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180
           GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR
Sbjct: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180

Query: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240
           AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE
Sbjct: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240

Query: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300
           ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI
Sbjct: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300

Query: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360
           KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ
Sbjct: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360

Query: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420
           DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL
Sbjct: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420

Query: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480
           DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG
Sbjct: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480

Query: 481 SVRYAGASFRSP 493
           SVRYAGASFRSP
Sbjct: 481 SVRYAGASFRSP 492

BLAST of CsaV3_1G003090 vs. ExPASy TrEMBL
Match: A0A5A7US84 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003390 PE=3 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 8.2e-269
Identity = 484/496 (97.58%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIP----SSSSSSSSKPLRSPTIRCSVAVAPSV 60
           MASLPSP TMNLLNK LSLSPPQSFI  IP    SSSSSSSSKPLRSPTIRCSVAVAPSV
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSL 180
           TVYGGKGVLTAVKNI DILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFT EGKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 493
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of CsaV3_1G003090 vs. ExPASy TrEMBL
Match: A0A1S3BX56 (Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103494088 PE=3 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 8.2e-269
Identity = 484/496 (97.58%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIP----SSSSSSSSKPLRSPTIRCSVAVAPSV 60
           MASLPSP TMNLLNK LSLSPPQSFI  IP    SSSSSSSSKPLRSPTIRCSVAVAPSV
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSL 180
           TVYGGKGVLTAVKNI DILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFT EGKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 493
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of CsaV3_1G003090 vs. ExPASy TrEMBL
Match: A0A5D3DYS6 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00060 PE=3 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 1.1e-268
Identity = 484/496 (97.58%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIP----SSSSSSSSKPLRSPTIRCSVAVAPSV 60
           MASLPSP TMNLLNK LSLSPPQSFI  IP    SSSSSSSSKPLRSPTIRCSVAVAPSV
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSL 180
           TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFT  GKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 493
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of CsaV3_1G003090 vs. ExPASy TrEMBL
Match: A0A6J1EV05 (Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111437858 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 9.1e-252
Identity = 456/494 (92.31%), Postives = 466/494 (94.33%), Query Frame = 0

Query: 1   MASLPSP--HTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVST 60
           MASLPSP   T NLLNKSL  S PQS I  IP   SSSSSKPLRS  +RCSVAVAPS ST
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIP---SSSSSKPLRSHGVRCSVAVAPSAST 60

Query: 61  NVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTV 120
             S EFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK V
Sbjct: 61  KASSEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNV 120

Query: 121 YGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSV 180
           YGGKGVLTAVKNINDILAPKLVGVDVRNQE+VDAIMLEIDGT NKS+LGANAILGVSLSV
Sbjct: 121 YGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSV 180

Query: 181 CRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSF 240
           CRAGAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+SF
Sbjct: 181 CRAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASF 240

Query: 241 AEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTG 300
           AEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTG
Sbjct: 241 AEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTG 300

Query: 301 KIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPF 360
           KIKIGMDVAASEFFT +G+YDLNFKKQPNDGAHVHSAHGLGELYKQFV DFPIVSIEDPF
Sbjct: 301 KIKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPF 360

Query: 361 DQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQA 420
           DQDDWSSWASLQSSV +Q+VGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESIQA
Sbjct: 361 DQDDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQA 420

Query: 421 ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE 480
           ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE
Sbjct: 421 ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE 480

Query: 481 LGSVRYAGASFRSP 493
           LG+VRYAG SFRSP
Sbjct: 481 LGNVRYAGESFRSP 491

BLAST of CsaV3_1G003090 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 783.1 bits (2021), Expect = 1.3e-226
Identity = 391/459 (85.19%), Postives = 427/459 (93.03%), Query Frame = 0

Query: 34  SSSSSKPLRSPTIRCSVAVAPSVSTNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLY 93
           S+ S    R   ++CSV         V+KE ++K +KARQIIDSRGNPTVEVDLITDDLY
Sbjct: 28  SAPSCLRFRRSGVQCSV---------VAKECRVKGVKARQIIDSRGNPTVEVDLITDDLY 87

Query: 94  RSAVPSGASTGIYEALELRDGDKTVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAI 153
           RSAVPSGASTGIYEALELRDGDK+VYGGKGVL A+KNIN+++APKL+GVDVRNQ +VDA+
Sbjct: 88  RSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAIKNINELVAPKLIGVDVRNQADVDAL 147

Query: 154 MLEIDGTPNKSELGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 213
           MLE+DGTPNKS+LGANAILGVSLSVCRAGAGAKG+PLY+HIQE SGTKELVMPVPAFNVI
Sbjct: 148 MLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVPLYKHIQETSGTKELVMPVPAFNVI 207

Query: 214 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 273
           NGGSHAGN+LAMQEFMILPVGA+SF+EA +MGSEVYHTLKGIIK KYGQDACNVGDEGGF
Sbjct: 208 NGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGF 267

Query: 274 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVH 333
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFF  +G+YDLNFKKQPNDGAHV 
Sbjct: 268 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVL 327

Query: 334 SAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 393
           SA  L +LY++F+KDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE 
Sbjct: 328 SAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEA 387

Query: 394 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 453
           I+K++CNALLLKVNQIG+VTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 388 IKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 447

Query: 454 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 493
           QIKTGAPCRSERL+KYNQLLRIEEELG+VRYAG +FRSP
Sbjct: 448 QIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEAFRSP 477

BLAST of CsaV3_1G003090 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 575.1 bits (1481), Expect = 5.4e-164
Identity = 295/435 (67.82%), Postives = 349/435 (80.23%), Query Frame = 0

Query: 69  LKARQIIDSRGNPTVEVDLITDDLYR--SAVPSGASTGIYEALELRDGDKTVYGGKGVLT 128
           +KARQI DSRGNPTVEVD+ T +  +  +AVPSGASTGIYEALELRDG    Y GKGV  
Sbjct: 7   VKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSD-YLGKGVSK 66

Query: 129 AVKNINDILAPKLVGVDVRNQEEVDAIML-EIDGTPN-----KSELGANAILGVSLSVCR 188
           AV N+N+I+ P L+G D   Q  +D  M+ E+DGT N     K +LGANAIL VSL+VC+
Sbjct: 67  AVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLAVCK 126

Query: 189 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 248
           AGA   G+PLY+HI  L+G  ++V+PVPAFNVINGGSHAGN LAMQEFMILPVGA+SF E
Sbjct: 127 AGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFKE 186

Query: 249 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 308
           A++MG EVYH LK +IK KYGQDA NVGDEGGFAPN+Q+N+EGL LL  AIEKAGYTGK+
Sbjct: 187 AMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGYTGKV 246

Query: 309 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 368
            IGMDVAASEF++ +  YDLNFK++ N+G+   S   L +LYK FV ++PIVSIEDPFDQ
Sbjct: 247 VIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDPFDQ 306

Query: 369 DDWSSWASL--QSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQA 428
           DDW  +A +  +   ++Q+VGDDLLVTNPKR+A+ I +K+CNALLLKVNQIGSVTESI+A
Sbjct: 307 DDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVTESIEA 366

Query: 429 ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE 488
              SK AGWGVM SHRSGETED FIADL+VGL++GQIKTGAPCRSERLAKYNQLLRIEEE
Sbjct: 367 VKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRIEEE 426

Query: 489 LGS-VRYAGASFRSP 493
           LGS   YAG +FR P
Sbjct: 427 LGSEAIYAGVNFRKP 440

BLAST of CsaV3_1G003090 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 464.5 bits (1194), Expect = 1.0e-130
Identity = 233/427 (54.57%), Postives = 307/427 (71.90%), Query Frame = 0

Query: 66  LKSLKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKTVYGGKGV 125
           +  +KARQI+DSRG PTVEVDL T+  ++R++VPSG S+G YEA+ELRDGDK +Y G  V
Sbjct: 47  ITKVKARQILDSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSV 106

Query: 126 LTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCRAGAG 185
             AVKNIN+ ++  L+G+D + Q ++D  M+++D T  KSELGANAIL VS++ C+AGA 
Sbjct: 107 AKAVKNINEKISEALIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAA 166

Query: 186 AKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEALRM 245
            K +PL +H+ +LSG   +V+PVPAF V++GG HA N  A+QE MILP+GAS F EAL+ 
Sbjct: 167 EKEVPLCKHLSDLSGRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQW 226

Query: 246 GSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGM 305
           GSE YH LK +I  K G   CNVG++GG AP++   +EGL L+ +AI + GY  KIKI +
Sbjct: 227 GSETYHHLKAVISEKNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAI 286

Query: 306 DVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDDWS 365
           D+AA+ F     KYDL+ K     G +  SA  + ++YK+   D+PIVSIEDPFD++DW 
Sbjct: 287 DIAATNFCLGT-KYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWE 346

Query: 366 SWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAALDSKA 425
                 S    Q+VGDDLL++N KR+   IQ+ +CNALLLKVNQIG+VTE+I+    ++ 
Sbjct: 347 HTKYFSSLGICQVVGDDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARD 406

Query: 426 AGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG-SVR 485
           A WGV+ SHR GETED+FI+DLSVGLA+G IK GAPCR ER  KYNQLLRIEEELG    
Sbjct: 407 AQWGVVTSHRCGETEDSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAV 466

Query: 486 YAGASFR 491
           YAG  ++
Sbjct: 467 YAGEDWK 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004138202.11.4e-275100.00enolase 1, chloroplastic [Cucumis sativus] >KGN63743.1 hypothetical protein Csa_... [more]
XP_008453338.11.7e-26897.58PREDICTED: enolase 1, chloroplastic [Cucumis melo] >KAA0058034.1 enolase 1 [Cucu... [more]
TYK28380.12.2e-26897.58enolase 1 [Cucumis melo var. makuwa][more]
XP_038879680.15.3e-26295.73enolase 1, chloroplastic [Benincasa hispida][more]
KAG6587623.11.1e-25192.31Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9C9C41.8e-22585.19Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1[more]
Q54RK51.7e-17070.07Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1[more]
P154297.9e-16870.07Beta-enolase OS=Rattus norvegicus OX=10116 GN=Eno3 PE=1 SV=3[more]
Q275271.0e-16770.30Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3[more]
P428941.0e-16769.35Enolase OS=Neocallimastix frontalis OX=4757 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LPK66.9e-276100.00Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G014400 PE=3 SV=1[more]
A0A5A7US848.2e-26997.58Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A1S3BX568.2e-26997.58Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103494088 PE=3 SV=1[more]
A0A5D3DYS61.1e-26897.58Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1EV059.1e-25292.31Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111437858 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G74030.11.3e-22685.19enolase 1 [more]
AT2G36530.15.4e-16467.82Enolase [more]
AT2G29560.11.0e-13054.57cytosolic enolase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000941EnolasePRINTSPR00148ENOLASEcoord: 224..237
score: 77.06
coord: 402..416
score: 80.2
coord: 97..111
score: 88.38
coord: 379..390
score: 78.16
coord: 167..183
score: 76.74
coord: 431..448
score: 83.0
IPR000941EnolaseTIGRFAMTIGR01060TIGR01060coord: 67..488
e-value: 2.3E-166
score: 551.6
IPR000941EnolaseSFLDSFLDF00002enolasecoord: 67..479
e-value: 0.0
score: 623.6
IPR000941EnolasePANTHERPTHR11902ENOLASEcoord: 30..492
IPR000941EnolaseHAMAPMF_00318Enolasecoord: 64..479
score: 43.597141
IPR000941EnolaseCDDcd03313enolasecoord: 68..478
e-value: 0.0
score: 740.836
IPR020811Enolase, N-terminalSMARTSM01193Enolase_N_3coord: 66..194
e-value: 3.8E-84
score: 295.5
IPR020811Enolase, N-terminalPFAMPF03952Enolase_Ncoord: 67..194
e-value: 2.9E-55
score: 186.0
IPR020810Enolase, C-terminal TIM barrel domainSMARTSM01192Enolase_C_3coord: 202..492
e-value: 6.9E-194
score: 660.1
IPR020810Enolase, C-terminal TIM barrel domainPFAMPF00113Enolase_Ccoord: 203..490
e-value: 5.6E-147
score: 488.7
IPR029017Enolase-like, N-terminalGENE3D3.30.390.10coord: 60..187
e-value: 2.6E-55
score: 188.1
IPR029017Enolase-like, N-terminalSUPERFAMILY54826Enolase N-terminal domain-likecoord: 66..197
IPR036849Enolase-like, C-terminal domain superfamilyGENE3D3.20.20.120coord: 188..492
e-value: 3.1E-152
score: 508.0
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILY51604Enolase C-terminal domain-likecoord: 202..485
NoneNo IPR availableSFLDSFLDG00178enolasecoord: 67..479
e-value: 0.0
score: 623.6
NoneNo IPR availablePANTHERPTHR11902:SF42ENOLASE 1, CHLOROPLASTICcoord: 30..492
IPR020809Enolase, conserved sitePROSITEPS00164ENOLASEcoord: 402..415

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G003090.1CsaV3_1G003090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0000015 phosphopyruvate hydratase complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004634 phosphopyruvate hydratase activity