CsGy7G020810 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy7G020810
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	RING-type E3 ubiquitin transferase
Location	Gy14Chr7: 23109421 .. 23113977 (+)
RNA-Seq Expression	CsGy7G020810
Synteny	CsGy7G020810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGGCTGATTGCCCAAGGAAAAGTTCTGCTCTACCAATTTCCCTATTTCTGCTCTGCTCTTCCATTTTGGTTGCCCATAACTGCGACAGCGGGCGTGTCTTCTTCACTGCCTCGGCAGCGGTGCTGGTCGTCGTCTCCACCGCATTCAAGGAGGGAGCTATTTTCACCGTCACAGAAACACGGAATTTCCTCATGTCCACATCGGAGCAAGCCCTTGTTACTTTACTCTCTCGTTTTGCTTTATCGTTTGATGGAGCCGTTTTAGGCGCTGCCCTAGCTTATGCTACCGTCCGTACCGTCCTTAAACTTACTGCTTCTTGCTCCGCTCTCAATGAGCTCCGTAAAGCTCCTTATGTTACTGTTTCCGATCTTCGTCATATCGTTTCCGACGGAGTTTCGGAACAATCCGAGTCTGATCGGAAGCTTGTTGTTGTTCGTGGCACCGTGGAGACTAGGTCGTCTGTGGAAGGTAATAGGAAGAGCTTACAGCCTAATGTTTTGATTTCTCAGGAGTCTGGAGATAGAGCGGTGATTATTCAGCGAACTCAAGTGGTAACTCCTTTGAGCGACTGTGTTGAGATTTTGTTTTGTTATTTCGGGGTTAGAAAATTGCATGATTGAAAATTATTATTCATCCGTTTGTTAGTAGGTTGTGCTTATAGAACGTTGTGTGGCAGTATTCGAGTAATTTGATGGCTCCACTGAAATTGTCGACAGAGTCTAACCAATTAATCGACTAACTTGTACAGATTTAGAATTTTCACATTACAAGGTTTCTAGTCTAGGTACTCGGTTCAAGACGTAGCTACTATACAATTCTATCCTGGATGAACCTCGAAAGGGAAGAGTCATCGAGTGAAGTAGCTGTTTAATTTATCTTTATAGGTCTTCTGGCTTGCCTGAAGTTGATCTGTCATCATCGTGTGTGCCTGTGTGTATATAATGTGGGGATTAAGTAGTTGGGAAATCTTGAAACTACTTAATCAACACGACTTCTTTATGTTCTGCAAGTGTTTGTTTTCGTTCCCCTTGGTTGTTTAATGTGATGGACATGTGCATATAATAATTCTGTTCTTTAAACTAAAGCTTTTTTGGTTGTATTGTGGCTTTGTTTAGTGCATATTCAACGAATGGAAAGGCTTTTTCGGATGGACTTCTGACTTGCGTGCAATTTTTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTAAATTCAAGGCTTATATCTGTTATTGTTATGTTTGATTTGCTCTTTTCATTACCCTTGCCAAAAGGGAAAAAAAAAGAAGATTTTTTAAATACCTTTTTATTGAATGTTACGGGGCTTTTTGATTATTTTCGTAAATAAAGAACTAACTAATTGAAGTGAGTGATTTTATGAGGGCATCTCTCTCAAATTTGGGGCCATAGAGTTGTGCCCTTTTCCGGTGATTCTCTATTGGGTCATTTTTTCTATCTCGTTGATGGCATGTGCAAGTAGGTTTGAAAGTCATACCTCTTAAGAAATGGATCCAACAGAGTGGCTTGAAGACGTGCCCCTTAGTGTCAAGAAATAAATATTATGGTAGATTACTTATTGTCTACAAAAACTAAATCTTCATGATATGACATTAATGTGTGGTCGTTGTGATTTGTGAAGTAAAATATACACTCTAGTCTTTAGATAGGGAACTATATTCAGTGCAGTTTGCGCATGAAGAGTTATGTGATACTGATATTTGGGAAATTTTGAACAGGTTCCTTTTGTTCTTCTTGAGGGTCATTCAACATACTCTGATTTTGTTGTTGTTAACATGGAAGGCTCAAGACATCCCTTACCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCACGAGTATCCTGTGAGTTAGGCAATCTATATTGAAGTCGAGCATCACTTTTTTTTTTCTTTTTTATAAATAGATTTTAGGTAAAATTCTTAGTTATGCTCTACTTTTGAAGGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGTATATGCAGTTTTGAAAATGGAGTTCCTGTCATAAAGTCTTGCAGTGATTTTCCCCATTTCCTGTAAGTTTATACATTTCAAAGTAGATCATAGATGCACATCATATCTTATTTCTCTCAACTTGTGGACATAAAATTGATGATAAATTTGTTCTGTCGGCCAATGGTGAGCCCTGTTGATTGGGTTTGGGGCTCTGGATGGGACTTCAACTAGGTCTTAGGATTAAGCCTCCGAAAGCTTTTGTTGTCTTTTGGAGCTGCATGGGTTTGTTTGTATTTGTTGTCTGGGTCCCAATACTCTCTTCTTCATACAAATGTGTGTGTGTGTTGTGCACGTTCTCATTTCTTGTTCAAGTGTGGGCCTTGGTTTCCTAAATCATGGAACAGATCTTGTTTCGTTAGCACTGCTAGCTAGTGGTTAGTCTCTCCTCTCCCGCATACCCAAAGTGAGAAAAAAAAAAACTGTAGGCAACTAATTGAAGCCATGTCTGATGTGTGACATTCATGCAATTTCCTCTTTGTTGTATATGGAGAACATTGTCTAATTGGACAACAAAAAATCGTTTCCTTTATGTTTTCCTTGTATTTGGTATTTTGAGTACTAGCCTCTTTTCATCTATTCATTCAAAAGAAAGAACTGTCTAATTGGACAAAAATTGTTCCTGATTACGCTTTCAAGACAATCTTCATTTAAATATTAATAACAAATATTATGATGATGTATTTTTCTCTGCAGATGTGAAATGACTAAGGATCAGATGATTTTAGATCTAGTCTTTAAAACAAAATTTCTATTTTGGAGTAGTATTGTCCTTGGTTCATTGACAGTTGGCATTCTTGGTTATTCTGCTGCGAGGTATGTTTTATTTTGTTAATTTTTTCTTTTATTTTATTTGGTTGAGGTATCTGTAAGGTCACATCCTCATGATTTGATATTCATATGATAATAACAAAAGTAAAATAGAGTCCGACTAACACAAGAATTACGAACAAAACAAACAAACCCCAGCCAAGCGAGAGGCTTGCACTTTTCCATGGTTGGACTATTCTTTACACACTAAACACAATATAACAAACCCTAGCCAAAATGATAGTTTATATACAAACATTCCCCTGCAACACGTACATCCAGGACTACAACCATCGAACCCTCCATCTGGGGTGTAATCCCCTTTCTACTCTTCCTCTTATATACTTTCTTAATGGAAGGCCTATCTGTACCTTTGTGCTGATTCTTGATATTTTTCTTTAGGCAAAGCAATACCTTGGCTTAATATACCTTCCATGAATTCTTTTCTTTTTTTCTGCTTTGCACTATACTTTTTGAATATCTTTCATTAGTCCTTTTATTAGACTATTCCGAAGTATAATACCCCACTCGTCTTATTGCTTCCTTCTTTCTATAATCACATTTCGAAAAGTTTAAGGAGTGCCAATATCCATTCTCTTCAGAAATTGGAATAGATGGAAACAATGGAGGCAACATAGACAGTTACAGAATTCAAGGAATGATTCCCCCACGTCACAACTGGATTCAAGCAATGACATTCCTATTTACGGTGATTTTGCTTCAGACGACGAGGCAGCAACTGTTCCAGACGATGAGTTATCATCACACGTTCCAGACGATGAGTTATCATCACACGTTCCAGACGATGAGTTATCATCACACGTTCCAGATGGACAATTGTGTGTGATCTGTCTCATGAGGAGAAAACGATCTGCTTTCATTCCATGCGGTCATCTAGTTTGTTGCGAACGATGTGCTGTGTCAGTCGAACGTGAGTCATCTCCCAAATGTCCCATTTGCCGCCAACAAATTCGTAGTTCTGTACGGATTTACGATTCGTGAAGAGACAAATCATCTTTTCAGGTTCTAACTACGACAAATCCCGTTTTGCTGATATTATACTGTTTTGAGATTTCATATAAATGCCAATCTCTTCCACAAGATAGCAGATGTGAAGTTTGTTTTTCCTCATCTACTTGTAACTTTTTATCCCCACATTTTGATGAGAGGGATTGTTCTTTAAAGTTTGTAAAGCCAGATTTGAAGTAATGGCTCTTGTAAATATCAGATAGCATATACTCTTTTGATATGTGACATGAAAAAAATGAAACTTTATTATTGGCTCCGTTATGTATAGGTGTATTGCTCGGATTGACTTTCCAAATGGTTTAGAAAAATAAATATGATTGGTTTTTTATGTCTTTGTTTGGACTTGTTTTTTCAGTGCACAGTTATATTCAAAACAAAGTTAAACCAAGTTGATCACAGTACTCAATTAAGAAGATCTCGAGGTCTAGCTTTGATGGGTTTATATATATATGTTGACTGAAAATAAGATTTTTGGATCCTTCAAATCTCGTTTTTGTGTACTTTCAAGAGGAAAAAAGAAACTCGTAAGAAGTCACAATAGCTCTTGAAATGAGAGGGATTTTTGAAGCCTTAATTTCAATTGGACTCACTTGAAAACACAAGC

mRNA sequence

CGGGCTGATTGCCCAAGGAAAAGTTCTGCTCTACCAATTTCCCTATTTCTGCTCTGCTCTTCCATTTTGGTTGCCCATAACTGCGACAGCGGGCGTGTCTTCTTCACTGCCTCGGCAGCGGTGCTGGTCGTCGTCTCCACCGCATTCAAGGAGGGAGCTATTTTCACCGTCACAGAAACACGGAATTTCCTCATGTCCACATCGGAGCAAGCCCTTGTTACTTTACTCTCTCGTTTTGCTTTATCGTTTGATGGAGCCGTTTTAGGCGCTGCCCTAGCTTATGCTACCGTCCGTACCGTCCTTAAACTTACTGCTTCTTGCTCCGCTCTCAATGAGCTCCGTAAAGCTCCTTATGTTACTGTTTCCGATCTTCGTCATATCGTTTCCGACGGAGTTTCGGAACAATCCGAGTCTGATCGGAAGCTTGTTGTTGTTCGTGGCACCGTGGAGACTAGGTCGTCTGTGGAAGGTAATAGGAAGAGCTTACAGCCTAATGTTTTGATTTCTCAGGAGTCTGGAGATAGAGCGGTGATTATTCAGCGAACTCAAGTGTGCATATTCAACGAATGGAAAGGCTTTTTCGGATGGACTTCTGACTTGCGTGCAATTTTTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTTCCTTTTGTTCTTCTTGAGGGTCATTCAACATACTCTGATTTTGTTGTTGTTAACATGGAAGGCTCAAGACATCCCTTACCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCACGAGTATCCTGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGTATATGCAGTTTTGAAAATGGAGTTCCTGTCATAAAGTCTTGCAGTGATTTTCCCCATTTCCTATGTGAAATGACTAAGGATCAGATGATTTTAGATCTAGTCTTTAAAACAAAATTTCTATTTTGGAGTAGTATTGTCCTTGGTTCATTGACAGTTGGCATTCTTGGTTATTCTGCTGCGAGAAATTGGAATAGATGGAAACAATGGAGGCAACATAGACAGTTACAGAATTCAAGGAATGATTCCCCCACGTCACAACTGGATTCAAGCAATGACATTCCTATTTACGGTGATTTTGCTTCAGACGACGAGGCAGCAACTGTTCCAGACGATGAGTTATCATCACACGTTCCAGACGATGAGTTATCATCACACGTTCCAGACGATGAGTTATCATCACACGTTCCAGATGGACAATTGTGTGTGATCTGTCTCATGAGGAGAAAACGATCTGCTTTCATTCCATGCGGTCATCTAGTTTGTTGCGAACGATGTGCTGTGTCAGTCGAACGTGAGTCATCTCCCAAATGTCCCATTTGCCGCCAACAAATTCGTAGTTCTGTACGGATTTACGATTCGTGAAGAGACAAATCATCTTTTCAGGTTCTAACTACGACAAATCCCGTTTTGCTGATATTATACTGTTTTGAGATTTCATATAAATGCCAATCTCTTCCACAAGATAGCAGATGTGAAGTTTGTTTTTCCTCATCTACTTGTAACTTTTTATCCCCACATTTTGATGAGAGGGATTGTTCTTTAAAGTTTGTAAAGCCAGATTTGAAGTAATGGCTCTTGTAAATATCAGATAGCATATACTCTTTTGATATGTGACATGAAAAAAATGAAACTTTATTATTGGCTCCGTTATGTATAGGTGTATTGCTCGGATTGACTTTCCAAATGGTTTAGAAAAATAAATATGATTGGTTTTTTATGTCTTTGTTTGGACTTGTTTTTTCAGTGCACAGTTATATTCAAAACAAAGTTAAACCAAGTTGATCACAGTACTCAATTAAGAAGATCTCGAGGTCTAGCTTTGATGGGTTTATATATATATGTTGACTGAAAATAAGATTTTTGGATCCTTCAAATCTCGTTTTTGTGTACTTTCAAGAGGAAAAAAGAAACTCGTAAGAAGTCACAATAGCTCTTGAAATGAGAGGGATTTTTGAAGCCTTAATTTCAATTGGACTCACTTGAAAACACAAGC

Coding sequence (CDS)

ATGTCCACATCGGAGCAAGCCCTTGTTACTTTACTCTCTCGTTTTGCTTTATCGTTTGATGGAGCCGTTTTAGGCGCTGCCCTAGCTTATGCTACCGTCCGTACCGTCCTTAAACTTACTGCTTCTTGCTCCGCTCTCAATGAGCTCCGTAAAGCTCCTTATGTTACTGTTTCCGATCTTCGTCATATCGTTTCCGACGGAGTTTCGGAACAATCCGAGTCTGATCGGAAGCTTGTTGTTGTTCGTGGCACCGTGGAGACTAGGTCGTCTGTGGAAGGTAATAGGAAGAGCTTACAGCCTAATGTTTTGATTTCTCAGGAGTCTGGAGATAGAGCGGTGATTATTCAGCGAACTCAAGTGTGCATATTCAACGAATGGAAAGGCTTTTTCGGATGGACTTCTGACTTGCGTGCAATTTTTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTTCCTTTTGTTCTTCTTGAGGGTCATTCAACATACTCTGATTTTGTTGTTGTTAACATGGAAGGCTCAAGACATCCCTTACCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCACGAGTATCCTGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGTATATGCAGTTTTGAAAATGGAGTTCCTGTCATAAAGTCTTGCAGTGATTTTCCCCATTTCCTATGTGAAATGACTAAGGATCAGATGATTTTAGATCTAGTCTTTAAAACAAAATTTCTATTTTGGAGTAGTATTGTCCTTGGTTCATTGACAGTTGGCATTCTTGGTTATTCTGCTGCGAGAAATTGGAATAGATGGAAACAATGGAGGCAACATAGACAGTTACAGAATTCAAGGAATGATTCCCCCACGTCACAACTGGATTCAAGCAATGACATTCCTATTTACGGTGATTTTGCTTCAGACGACGAGGCAGCAACTGTTCCAGACGATGAGTTATCATCACACGTTCCAGACGATGAGTTATCATCACACGTTCCAGACGATGAGTTATCATCACACGTTCCAGATGGACAATTGTGTGTGATCTGTCTCATGAGGAGAAAACGATCTGCTTTCATTCCATGCGGTCATCTAGTTTGTTGCGAACGATGTGCTGTGTCAGTCGAACGTGAGTCATCTCCCAAATGTCCCATTTGCCGCCAACAAATTCGTAGTTCTGTACGGATTTACGATTCGTGA

Protein sequence

MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDLRHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQVCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWRQHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS*

Homology

[Close]

BLAST of CsGy7G020810 vs. ExPASy Swiss-Prot
Match: Q9SYH3 (E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 9.7e-98
Identity = 204/430 (47.44%), Postives = 279/430 (64.88%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+ E+AL+ LL+  ALSFDGA+LG  LA + V + LK  ++ +AL +++ AP V++SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHIV--SDGVSEQSE----SDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVI 120
           R ++  S+  SE ++    +D+++VVVRG V+ +  + G+      NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPK--ISGDEGYKNNNVLISPETGDKALI 120

Query: 121 IQRTQVCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVN 180
           IQRTQ  +++ WK  F  T   R +  R+ RK  +   RTVPFV++ +   + S FV VN
Sbjct: 121 IQRTQTYVYSGWKRLFQSTGH-RFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSFVAVN 180

Query: 181 MEGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICS 240
           M+GSR PLPLTTVY++LQP+ +   +FLQA    +YPVG+LD EKILP GK+I+AVGI S
Sbjct: 181 MDGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYS 240

Query: 241 FENGVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNW 300
           F NGVP IKSC D P+FL EMTKD+MI DL+ +T F+F  S++LG ++VGIL Y+A R W
Sbjct: 241 FNNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTW 300

Query: 301 NRWKQWRQHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELS 360
           N+WKQW   R+L    NDS                                  V DDE  
Sbjct: 301 NKWKQWNHQRELPQRPNDS----------------------------------VVDDE-- 360

Query: 361 SHVPDDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQ 420
              P+D  +  +PDG+LCVIC+ RR+  AFIPCGH+VCC RCA +VERE +PKCP+C Q 
Sbjct: 361 ---PED--ADEIPDGELCVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQS 383

Query: 421 IRSSVRIYDS 424
           IR S+R+Y S
Sbjct: 421 IRGSMRVYYS 383

[Close]

BLAST of CsGy7G020810 vs. ExPASy Swiss-Prot
Match: P41437 (E3 ubiquitin-protein ligase IAP-3 OS=Orgyia pseudotsugata multicapsid polyhedrosis virus OX=262177 GN=IAP3 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.2e-08
Identity = 29/75 (38.67%), Postives = 41/75 (54.67%), Query Frame = 0


Query: 347 VPDDELSSHVPDDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPK 406
           V D +   H+    + + V D +LC ICL   K   F+PCGH+V C +CA  V       
Sbjct: 197 VRDADNEPHIERPAVEAEVADDRLCKICLGAEKTVCFVPCGHVVACGKCAAGV-----TT 256

Query: 407 CPICRQQIRSSVRIY 422
           CP+CR Q+  +VR+Y
Sbjct: 257 CPVCRGQLDKAVRMY 266

[Close]

BLAST of CsGy7G020810 vs. ExPASy Swiss-Prot
Match: Q24306 (Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=Diap1 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.2e-07
Identity = 26/62 (41.94%), Postives = 38/62 (61.29%), Query Frame = 0


Query: 362 SSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIY 421
           S+ +P+ +LC IC      +AF+PCGH+V C +CA SV      KCP+CR+     +R+Y
Sbjct: 382 STSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSV-----TKCPLCRKPFTDVMRVY 438

Query: 422 DS 424
            S
Sbjct: 442 FS 438

[Close]

BLAST of CsGy7G020810 vs. ExPASy Swiss-Prot
Match: P41436 (Apoptosis inhibitor IAP OS=Cydia pomonella granulosis virus (isolate Mexico/1963) OX=654905 GN=IAP PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.1e-07
Identity = 32/94 (34.04%), Postives = 54/94 (57.45%), Query Frame = 0


Query: 334 EAATVPDDE--LSSHVPDDELSSHVPDDELSS--HVPDGQLCVICLMRRKRSAFIPCGHL 393
           EA  +P +   +S+  P   +S  +P+ ++     V D +LC IC +      F+PCGH+
Sbjct: 190 EACVLPGENTTVSTAAP---VSEPIPETKIEKEPQVEDSKLCKICYVEECIVCFVPCGHV 249

Query: 394 VCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 424
           V C +CA+SV+     KCP+CR+ + S +++Y S
Sbjct: 250 VACAKCALSVD-----KCPMCRKIVTSVLKVYFS 275

[Close]

BLAST of CsGy7G020810 vs. ExPASy Swiss-Prot
Match: Q90660 (Inhibitor of apoptosis protein OS=Gallus gallus OX=9031 GN=ITA PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 7.9e-07
Identity = 28/76 (36.84%), Postives = 45/76 (59.21%), Query Frame = 0


Query: 346 HVPDDELSSHVPDDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSP 405
           +VP +++S  +P +E    + + + C +C+ +     FIPCGHLV C+ CA S+      
Sbjct: 540 YVPTEDVSG-LPMEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKECAPSLR----- 599

Query: 406 KCPICRQQIRSSVRIY 422
           KCPICR  I+ +VR +
Sbjct: 600 KCPICRGTIKGTVRTF 609

[Close]

BLAST of CsGy7G020810 vs. NCBI nr
Match: XP_011659622.2 (E3 ubiquitin-protein ligase SPL2 isoform X1 [Cucumis sativus])

HSP 1 Score: 840 bits (2169), Expect = 5.11e-307
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDDE 360
           QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDDE
Sbjct: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDDE 360

Query: 361 LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 420
           LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI
Sbjct: 361 LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 420

Query: 421 YDS 423
           YDS
Sbjct: 421 YDS 423

[Close]

BLAST of CsGy7G020810 vs. NCBI nr
Match: XP_008462309.1 (PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo] >KAA0059400.1 mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo var. makuwa] >TYK03927.1 mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo var. makuwa])

HSP 1 Score: 776 bits (2004), Expect = 3.63e-282
Identity = 396/423 (93.62%), Postives = 401/423 (94.80%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIVSDG SEQSESDRKLVVVRGTVETRSSVEGN KSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL++GHSTYSDFVVVNMEGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSA RNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDDE 360
           QHR+LQNSRNDS TSQLDSSNDIPIYGDFASDDEAATV                  PDDE
Sbjct: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFASDDEAATV------------------PDDE 360

Query: 361 LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 420
           LSS+VPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVER+SSPKCPICRQQIRSSVRI
Sbjct: 361 LSSNVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRI 405

Query: 421 YDS 423
           YDS
Sbjct: 421 YDS 405

[Close]

BLAST of CsGy7G020810 vs. NCBI nr
Match: XP_038897215.1 (E3 ubiquitin-protein ligase SPL2 [Benincasa hispida])

HSP 1 Score: 725 bits (1872), Expect = 3.62e-262
Identity = 371/425 (87.29%), Postives = 381/425 (89.65%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYA VRTVLKLTASCSALNELRKAPYV VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVGVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+ DG S QSESDRKLVVVRGTVE RSSVEGN KSLQPN+LISQESGDRAVIIQRTQV
Sbjct: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNILISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL+EGHS YSDFVVVNM+GSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSL+VGILGYS  RNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDDELSSHVPD 360
           QHR+LQNS NDS TSQLDSSNDIPIYGDF  ASDDE ATV                    
Sbjct: 301 QHRRLQNSGNDSTTSQLDSSNDIPIYGDFGTASDDEVATV-------------------- 360

Query: 361 DELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSV 420
                  PDGQLCVICL RRKRSAFIPCGH VCCERCAVS+ER+S+PKCP+CRQQIRSSV
Sbjct: 361 -------PDGQLCVICLTRRKRSAFIPCGHFVCCERCAVSIERDSAPKCPVCRQQIRSSV 398

Query: 421 RIYDS 423
           RIYDS
Sbjct: 421 RIYDS 398

[Close]

BLAST of CsGy7G020810 vs. NCBI nr
Match: XP_023549225.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SPL2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 699 bits (1805), Expect = 8.13e-252
Identity = 359/426 (84.27%), Postives = 384/426 (90.14%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+SEQALV+LLS+FAL FDGAVLGAALAYA VRTVLKLTASCSAL+ELRKAPYV+VSDL
Sbjct: 1   MSSSEQALVSLLSQFALXFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+SDG S QSESD KLVVVRGTVE RSSVEGN KS+QPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVNMEGSRH 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL+ EGHS YSDFVVVNM+GSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVP 240
           PLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQW 300
           VIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS+VLGSL+VG+LGYS  RNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDDELSSHVP 360
           RQ R+LQ+S NDS  SQLDS NDIPIYGDF  ASD+E A                    P
Sbjct: 301 RQQRRLQDSGNDSTMSQLDS-NDIPIYGDFGTASDNEEAAD------------------P 360

Query: 361 DDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSS 420
           +DE+SS+VPDGQLCVICLMRRKRSAFIPCGH VCCERCA+SVER+S+PKCP+CRQQIRSS
Sbjct: 361 EDEVSSNVPDGQLCVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSS 407

Query: 421 VRIYDS 423
           VRIYDS
Sbjct: 421 VRIYDS 407

[Close]

BLAST of CsGy7G020810 vs. NCBI nr
Match: XP_022992269.1 (E3 ubiquitin-protein ligase SPL2 [Cucurbita maxima] >XP_022992271.1 E3 ubiquitin-protein ligase SPL2 [Cucurbita maxima])

HSP 1 Score: 698 bits (1802), Expect = 2.33e-251
Identity = 358/426 (84.04%), Postives = 385/426 (90.38%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+SEQALV+LLS+FALSFDGAVLGAALAYA VRTVLKLTASCSAL+ELRKAPYV+VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+SDG S +SESD KLVVVRGTVE RSSVEGN KS+QPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIISDGDSGKSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVNMEGSRH 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL+ EGHS YSDFVVVNM+GSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVP 240
           PLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQW 300
           VIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS+VLGSL+VG+LGYS  RNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDDELSSHVP 360
           RQ R+LQ+S NDS  SQLDS+N IPIYGDF  ASD+E A                    P
Sbjct: 301 RQQRRLQDSGNDSTMSQLDSNN-IPIYGDFGTASDNEEAVD------------------P 360

Query: 361 DDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSS 420
           +DE+SS+VPDGQLCVICLMRRKRSAFIPCGH VCCERCA+SVER+S+PKCP+CRQQIRSS
Sbjct: 361 EDEVSSNVPDGQLCVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSS 407

Query: 421 VRIYDS 423
           VRIYDS
Sbjct: 421 VRIYDS 407

[Close]

BLAST of CsGy7G020810 vs. ExPASy TrEMBL
Match: A0A0A0K708 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G450490 PE=4 SV=1)

HSP 1 Score: 794 bits (2051), Expect = 1.21e-289
Identity = 405/423 (95.74%), Postives = 405/423 (95.74%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDDE 360
           QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATV                  PDDE
Sbjct: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATV------------------PDDE 360

Query: 361 LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 420
           LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI
Sbjct: 361 LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 405

Query: 421 YDS 423
           YDS
Sbjct: 421 YDS 405

[Close]

BLAST of CsGy7G020810 vs. ExPASy TrEMBL
Match: A0A5D3BWQ0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001240 PE=4 SV=1)

HSP 1 Score: 776 bits (2004), Expect = 1.76e-282
Identity = 396/423 (93.62%), Postives = 401/423 (94.80%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIVSDG SEQSESDRKLVVVRGTVETRSSVEGN KSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL++GHSTYSDFVVVNMEGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSA RNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDDE 360
           QHR+LQNSRNDS TSQLDSSNDIPIYGDFASDDEAATV                  PDDE
Sbjct: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFASDDEAATV------------------PDDE 360

Query: 361 LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 420
           LSS+VPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVER+SSPKCPICRQQIRSSVRI
Sbjct: 361 LSSNVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRI 405

Query: 421 YDS 423
           YDS
Sbjct: 421 YDS 405

[Close]

BLAST of CsGy7G020810 vs. ExPASy TrEMBL
Match: A0A1S3CGP0 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500694 PE=4 SV=1)

HSP 1 Score: 776 bits (2004), Expect = 1.76e-282
Identity = 396/423 (93.62%), Postives = 401/423 (94.80%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIVSDG SEQSESDRKLVVVRGTVETRSSVEGN KSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL++GHSTYSDFVVVNMEGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSA RNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDDE 360
           QHR+LQNSRNDS TSQLDSSNDIPIYGDFASDDEAATV                  PDDE
Sbjct: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFASDDEAATV------------------PDDE 360

Query: 361 LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 420
           LSS+VPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVER+SSPKCPICRQQIRSSVRI
Sbjct: 361 LSSNVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRI 405

Query: 421 YDS 423
           YDS
Sbjct: 421 YDS 405

[Close]

BLAST of CsGy7G020810 vs. ExPASy TrEMBL
Match: A0A6J1JT39 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 PE=4 SV=1)

HSP 1 Score: 698 bits (1802), Expect = 1.13e-251
Identity = 358/426 (84.04%), Postives = 385/426 (90.38%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+SEQALV+LLS+FALSFDGAVLGAALAYA VRTVLKLTASCSAL+ELRKAPYV+VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+SDG S +SESD KLVVVRGTVE RSSVEGN KS+QPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIISDGDSGKSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVNMEGSRH 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL+ EGHS YSDFVVVNM+GSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVP 240
           PLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQW 300
           VIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS+VLGSL+VG+LGYS  RNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDDELSSHVP 360
           RQ R+LQ+S NDS  SQLDS+N IPIYGDF  ASD+E A                    P
Sbjct: 301 RQQRRLQDSGNDSTMSQLDSNN-IPIYGDFGTASDNEEAVD------------------P 360

Query: 361 DDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSS 420
           +DE+SS+VPDGQLCVICLMRRKRSAFIPCGH VCCERCA+SVER+S+PKCP+CRQQIRSS
Sbjct: 361 EDEVSSNVPDGQLCVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSS 407

Query: 421 VRIYDS 423
           VRIYDS
Sbjct: 421 VRIYDS 407

[Close]

BLAST of CsGy7G020810 vs. ExPASy TrEMBL
Match: A0A6J1GS48 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456504 PE=4 SV=1)

HSP 1 Score: 695 bits (1794), Expect = 1.86e-250
Identity = 357/426 (83.80%), Postives = 383/426 (89.91%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+SEQALV+LLS+FALSFDGAVLGAALAYA VRTVLKLTASCSAL+ELRKAPYV+VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+SDG S QSESD KLVVVRGTVE RSSVEGN KS+QPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVNMEGSRH 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTS RTVPFVL+ EGHS YSDFVVVNM+GSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSLRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVP 240
           PLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGK ISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKKISAVGICSFKNGVP 240

Query: 241 VIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQW 300
           VIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS+VLGSL+VG+LGYS  RNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDDELSSHVP 360
           RQ R+LQ+S NDS  SQLDS+N IPIYGDF  ASD+E A                    P
Sbjct: 301 RQQRRLQDSGNDSTMSQLDSNN-IPIYGDFGTASDNEEAAD------------------P 360

Query: 361 DDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSS 420
           +DE+SS+VPDGQLCVICLMRRKRSAFIPCGH VCCERCA+SVER+S+PKCP+CRQQIRSS
Sbjct: 361 EDEVSSNVPDGQLCVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSS 407

Query: 421 VRIYDS 423
           VRIYDS
Sbjct: 421 VRIYDS 407

[Close]

BLAST of CsGy7G020810 vs. TAIR 10
Match: AT1G54150.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 358.6 bits (919), Expect = 6.9e-99
Identity = 204/430 (47.44%), Postives = 279/430 (64.88%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+ E+AL+ LL+  ALSFDGA+LG  LA + V + LK  ++ +AL +++ AP V++SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHIV--SDGVSEQSE----SDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVI 120
           R ++  S+  SE ++    +D+++VVVRG V+ +  + G+      NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPK--ISGDEGYKNNNVLISPETGDKALI 120

Query: 121 IQRTQVCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVN 180
           IQRTQ  +++ WK  F  T   R +  R+ RK  +   RTVPFV++ +   + S FV VN
Sbjct: 121 IQRTQTYVYSGWKRLFQSTGH-RFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSFVAVN 180

Query: 181 MEGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICS 240
           M+GSR PLPLTTVY++LQP+ +   +FLQA    +YPVG+LD EKILP GK+I+AVGI S
Sbjct: 181 MDGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYS 240

Query: 241 FENGVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNW 300
           F NGVP IKSC D P+FL EMTKD+MI DL+ +T F+F  S++LG ++VGIL Y+A R W
Sbjct: 241 FNNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTW 300

Query: 301 NRWKQWRQHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELS 360
           N+WKQW   R+L    NDS                                  V DDE  
Sbjct: 301 NKWKQWNHQRELPQRPNDS----------------------------------VVDDE-- 360

Query: 361 SHVPDDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQ 420
              P+D  +  +PDG+LCVIC+ RR+  AFIPCGH+VCC RCA +VERE +PKCP+C Q 
Sbjct: 361 ---PED--ADEIPDGELCVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQS 383

Query: 421 IRSSVRIYDS 424
           IR S+R+Y S
Sbjct: 421 IRGSMRVYYS 383

[Close]

BLAST of CsGy7G020810 vs. TAIR 10
Match: AT4G24204.1 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 8.6e-09
Identity = 27/59 (45.76%), Postives = 32/59 (54.24%), Query Frame = 0


Query: 362 SSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 421
           S  V    LCVIC   R+   F+PCGH   C  CA  +  E S  CPICR+ IR S R+
Sbjct: 60  SDDVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 118

[Close]

BLAST of CsGy7G020810 vs. TAIR 10
Match: AT4G24204.2 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 8.6e-09
Identity = 27/59 (45.76%), Postives = 32/59 (54.24%), Query Frame = 0


Query: 362 SSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 421
           S  V    LCVIC   R+   F+PCGH   C  CA  +  E S  CPICR+ IR S R+
Sbjct: 94  SDDVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 152

[Close]

BLAST of CsGy7G020810 vs. TAIR 10
Match: AT4G24204.3 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 8.6e-09
Identity = 27/59 (45.76%), Postives = 32/59 (54.24%), Query Frame = 0


Query: 362 SSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 421
           S  V    LCVIC   R+   F+PCGH   C  CA  +  E S  CPICR+ IR S R+
Sbjct: 115 SDDVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 173

[Close]

BLAST of CsGy7G020810 vs. TAIR 10
Match: AT1G59560.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 53.9 bits (128), Expect = 3.6e-07
Identity = 20/52 (38.46%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 370 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIY 422
           LCV+CL ++  +AF+ CGH+ CC  C++ +       CP+CR++I+  ++IY
Sbjct: 290 LCVVCLDQKYNTAFVECGHMCCCTPCSLQLR-----TCPLCRERIQQVLKIY 336

The following BLAST results are available for this feature:

BLAST of CsGy7G020810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SYH3	9.7e-98	47.44	E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV...	[more]
P41437	4.2e-08	38.67	E3 ubiquitin-protein ligase IAP-3 OS=Orgyia pseudotsugata multicapsid polyhedros...	[more]
Q24306	1.2e-07	41.94	Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=...	[more]
P41436	2.1e-07	34.04	Apoptosis inhibitor IAP OS=Cydia pomonella granulosis virus (isolate Mexico/1963...	[more]
Q90660	7.9e-07	36.84	Inhibitor of apoptosis protein OS=Gallus gallus OX=9031 GN=ITA PE=2 SV=1	[more]

BLAST of CsGy7G020810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659622.2	5.11e-307	100.00	E3 ubiquitin-protein ligase SPL2 isoform X1 [Cucumis sativus]	[more]
XP_008462309.1	3.63e-282	93.62	PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo] >KA...	[more]
XP_038897215.1	3.62e-262	87.29	E3 ubiquitin-protein ligase SPL2 [Benincasa hispida]	[more]
XP_023549225.1	8.13e-252	84.27	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SPL2 [Cucurbita pepo subsp. pep...	[more]
XP_022992269.1	2.33e-251	84.04	E3 ubiquitin-protein ligase SPL2 [Cucurbita maxima] >XP_022992271.1 E3 ubiquitin...	[more]

BLAST of CsGy7G020810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K708	1.21e-289	95.74	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G450490 PE...	[more]
A0A5D3BWQ0	1.76e-282	93.62	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3CGP0	1.76e-282	93.62	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500694 PE=4 ...	[more]
A0A6J1JT39	1.13e-251	84.04	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 P...	[more]
A0A6J1GS48	1.86e-250	83.80	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456504...	[more]

BLAST of CsGy7G020810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G54150.1	6.9e-99	47.44	E3 Ubiquitin ligase family protein	[more]
AT4G24204.1	8.6e-09	45.76	RING/U-box superfamily protein	[more]
AT4G24204.2	8.6e-09	45.76	RING/U-box superfamily protein	[more]
AT4G24204.3	8.6e-09	45.76	RING/U-box superfamily protein	[more]
AT1G59560.1	3.6e-07	38.46	E3 Ubiquitin ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 371..410 e-value: 0.0021 score: 27.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 371..411 score: 10.984472
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 369..416 e-value: 4.8E-14 score: 51.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 339..361
None	No IPR available	PANTHER	PTHR47355:SF1	E3 UBIQUITIN-PROTEIN LIGASE SPL2	coord: 1..319 coord: 350..423
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 362..417
IPR022170	E3 Ubiquitin ligase MUL1-like	PFAM	PF12483	GIDE	coord: 142..283 e-value: 3.0E-33 score: 114.5
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 355..422 e-value: 3.0E-16 score: 60.6
IPR044247	E3 ubiquitin-protein ligase SPL2-like	PANTHER	PTHR47355	E3 UBIQUITIN-PROTEIN LIGASE SPL2	coord: 1..319 coord: 350..423

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy7G020810.1	CsGy7G020810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006996	organelle organization
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity