Homology
BLAST of CsGy7G014220 vs. ExPASy Swiss-Prot
Match:
P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)
HSP 1 Score: 268.5 bits (685), Expect = 1.1e-70
Identity = 143/329 (43.47%), Postives = 205/329 (62.31%), Query Frame = 0
Query: 6 AKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEF 65
A FVSNW T +SV ES++ P +KR VP+ +P+IDL + LLV++I A ++F
Sbjct: 2 ATFVSNWST-KSVSESFIAPLQKR-AEKDVPVGNDVPIIDLQ-QHHHLLVQQITKACQDF 61
Query: 66 GFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAK-----ECSK---DPNRSCRVYTSSE 125
G FQ+INHG +++ ETM + KEF A+ EK K E +K + ++Y E
Sbjct: 62 GLFQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGE 121
Query: 126 NYTKEQVHCWRDALIFNCHPLEK-YVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSE 185
+ E+ W+D L CHPL++ V+ WP+KP KYREVV Y + +RKL + +L+ + E
Sbjct: 122 QLSNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICE 181
Query: 186 GLGLGKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQ--VL 245
GLGL GYF E+S+ ++L N+YPPCP+PS TLG H D +LIT+L QD+ GLQ ++
Sbjct: 182 GLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIV 241
Query: 246 KDGQWIGVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAM 305
KD WI VQPI AFVVN+G L++ITN K + + HR VT+ R S+ L+ P
Sbjct: 242 KDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCT 301
Query: 306 MEPAKCMINEANPPRYRSLNFKDFQRNYL 324
+EPAK ++N+ NPP Y+ ++ +F YL
Sbjct: 302 IEPAKELLNQDNPPLYKPYSYSEFADIYL 327
BLAST of CsGy7G014220 vs. ExPASy Swiss-Prot
Match:
Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)
HSP 1 Score: 260.8 bits (665), Expect = 2.2e-68
Identity = 139/340 (40.88%), Postives = 205/340 (60.29%), Query Frame = 0
Query: 6 AKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEF 65
AK +S ++PE+YV P RP V + P+IDLS DR+ L+++I A F
Sbjct: 3 AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62
Query: 66 GFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVH 125
GFFQ+INHGV+K++ +E + + +EF +MS EK K S DP ++ R+ TS N KE+V+
Sbjct: 63 GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122
Query: 126 CWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 185
WRD L +C+P+ KYV+ WP PP ++E+V Y +R++ +I EL+SE LGL K Y
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182
Query: 186 GGEMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGV 245
+ E + VN+YPPCP P LT GL H DP+ +TIL QD V GLQ+L DGQW V
Sbjct: 183 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 242
Query: 246 QPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCM- 305
P +AFV+NIG LQ ++NG ++ HRAVTN++ R S+ + P D A+M PAK +
Sbjct: 243 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 302
Query: 306 --INEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETN 340
++ P Y+ + ++ + + R +D + ++ N
Sbjct: 303 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341
BLAST of CsGy7G014220 vs. ExPASy Swiss-Prot
Match:
Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)
HSP 1 Score: 249.6 bits (636), Expect = 5.1e-65
Identity = 128/326 (39.26%), Postives = 187/326 (57.36%), Query Frame = 0
Query: 16 ESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGFFQIINHGV 75
+++P YV P +RP +V IPV+DL+ DR +V + DA + GFFQ++NHG+
Sbjct: 18 DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77
Query: 76 SKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCWRDALIFNC 135
+ M + +EF + EKAK S DP + R+ TS N KE VH WRD L +C
Sbjct: 78 DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHC 137
Query: 136 HPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGGEMSENPL- 195
+PL ++V WP PP ++E++G YC +R+L + E +SE LGL GY + E
Sbjct: 138 YPLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQH 197
Query: 196 LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGVQPIDNAFVVN 255
+ VN+YP CP P LT GL H DP+ +TIL D V GLQVL DG+WI V P A V+N
Sbjct: 198 MAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVIN 257
Query: 256 IGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANPPRYRS 315
IG LQ ++NGK ++ HRAV NS R S+ + P + + PAK +I + +P YR+
Sbjct: 258 IGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRN 317
Query: 316 LNFKDFQRNYLPRAVDTKAVMQYIET 339
+ ++ + + R +D + ++ T
Sbjct: 318 YTYDEYYKKFWSRNLDQEHCLELFRT 342
BLAST of CsGy7G014220 vs. ExPASy Swiss-Prot
Match:
Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)
HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 127/312 (40.71%), Postives = 185/312 (59.29%), Query Frame = 0
Query: 17 SVPESYVYPPEKRPGNIVVPMAKAI---PVIDLSIRDRTLLVRKILDASKEFGFFQIINH 76
S+PES+V+ +K P P KA+ P+IDLS R + R IL+A KE GFFQ++NH
Sbjct: 13 SLPESFVFASDKVP-----PATKAVVSLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNH 72
Query: 77 GVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCWRDALIF 136
GVSK+V + + ++F + +KA S++ ++ R++ S Y WRD L
Sbjct: 73 GVSKQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLF-SGATYDTGGEKYWRDCLRL 132
Query: 137 NC-HPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGGEMS-E 196
C P++ ++ WP P R+V+ + R + E+L L+ EG+G+ YF G++S
Sbjct: 133 ACPFPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGG 192
Query: 197 NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPIDNAFV 256
N +L +NHYP CPNP LG HCD +LIT+L VNGL+V G WI V P NAFV
Sbjct: 193 NVILNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFV 252
Query: 257 VNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANPPRY 316
VN G L+++TNG L++ EHRA+TNS +R S+ + P E ++ PAK +++ NPP Y
Sbjct: 253 VNFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCY 312
Query: 317 RSLNFKDFQRNY 323
R+ F+DF R Y
Sbjct: 313 RTTMFRDFMRIY 317
BLAST of CsGy7G014220 vs. ExPASy Swiss-Prot
Match:
Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)
HSP 1 Score: 226.9 bits (577), Expect = 3.5e-58
Identity = 126/325 (38.77%), Postives = 190/325 (58.46%), Query Frame = 0
Query: 15 VESVPESYVYPPEKRPG-NIVVPMAKAIPVIDLSI---RDRTLLVRKILDASKEFGFFQI 74
V+ VP +YV P RP + V +IP+IDL +R ++ + A GFFQI
Sbjct: 15 VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74
Query: 75 INHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCWRDA 134
NHGV ++ ++ M +EF S E+ K S D ++ R+ TS N +KE+V WRD
Sbjct: 75 KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134
Query: 135 LIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGGEMS 194
L +C+P+E +++ WP P +REV Y ++R LVL +LE +SE LGL K +
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194
Query: 195 EN-PLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPIDNA 254
++ + +N+YP CP P LT GL H D +LIT+L QD V+GLQV KDG+WI V P+ N
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254
Query: 255 FVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA--N 314
F+VN+G +Q+I+N K ++ HRAV NS R S+ P ++A++ PA+ +INE +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314
Query: 315 PPRYRSLNFKDFQRNYLPRAVDTKA 332
P YR+ + ++ + A DT++
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTES 338
BLAST of CsGy7G014220 vs. NCBI nr
Match:
XP_004146985.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44748.1 hypothetical protein Csa_016058 [Cucumis sativus])
HSP 1 Score: 699 bits (1804), Expect = 3.64e-254
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0
Query: 1 MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD
Sbjct: 1 MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
Query: 61 ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT 120
ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT
Sbjct: 61 ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT 120
Query: 121 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180
KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL
Sbjct: 121 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180
Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
Query: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC
Sbjct: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
BLAST of CsGy7G014220 vs. NCBI nr
Match:
XP_008451261.1 (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])
HSP 1 Score: 665 bits (1715), Expect = 1.34e-240
Identity = 324/341 (95.01%), Postives = 328/341 (96.19%), Query Frame = 0
Query: 1 MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
M QG AKFVSNWET ESVPESYVYPPEKRPGN+VVPMAKAIPVIDLSI DR LLVRKIL
Sbjct: 1 MQQGDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILG 60
Query: 61 ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT 120
ASKE GFFQIINHGVSK+VSE TMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENY
Sbjct: 61 ASKESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYK 120
Query: 121 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180
KEQ+HCWRDALIFNCHPLEKYVHFWPQ PPKYREVVGAYC+AMRKLVLEILELMSEGLGL
Sbjct: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGL 180
Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
GKGY GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
Query: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
GVQPID AFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEA MEPAKC
Sbjct: 241 GVQPIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKC 300
Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
BLAST of CsGy7G014220 vs. NCBI nr
Match:
XP_038897058.1 (hyoscyamine 6-dioxygenase-like [Benincasa hispida])
HSP 1 Score: 643 bits (1658), Expect = 6.31e-232
Identity = 308/335 (91.94%), Postives = 323/335 (96.42%), Query Frame = 0
Query: 7 KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66
KFV+NWE V+SVPESYVYPP KRPGN+VVPM AIPVIDLS++DR LL+RKILDASKEFG
Sbjct: 6 KFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDASKEFG 65
Query: 67 FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHC 126
FFQ+INHGVSK VS ETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENY KEQ+HC
Sbjct: 66 FFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHC 125
Query: 127 WRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFG 186
WRDALIFNCHPLEKYVHFWPQ PPKYREVVGAYC+AMRKLVLEILELMSEGLGLGKGYFG
Sbjct: 126 WRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFG 185
Query: 187 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPID 246
GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDG+WIGVQPID
Sbjct: 186 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIGVQPID 245
Query: 247 NAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEAN 306
NAFVVNIGFVLQ+ITNGKL+AAEHRAVTN+KTSRQSLTYLVYPKDEA MEPAKCMINEAN
Sbjct: 246 NAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMINEAN 305
Query: 307 PPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
P RYRSLNFKDFQRNYLPRAVDTKAVM+YI +NQS
Sbjct: 306 PRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQS 340
BLAST of CsGy7G014220 vs. NCBI nr
Match:
XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 606 bits (1562), Expect = 2.59e-217
Identity = 287/338 (84.91%), Postives = 312/338 (92.31%), Query Frame = 0
Query: 4 GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
G KFVSNWE V+SVP YVYPPEKRPGN+VVPMA IPV+DLS RDR LLVRKILDAS+
Sbjct: 2 GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQ 61
Query: 64 EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
EFGFFQ+INHGVSK+V EET RIFK+FHAMSG EKAKECS+DPN+SCRVYTSSENY KEQ
Sbjct: 62 EFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQ 121
Query: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183
HCWRDALIFNCHPL+KYVHFWPQ PPKYREVVGAYC+ MR LVLEILEL+SEGLGLG+G
Sbjct: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRG 181
Query: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
Y GGEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 182 YLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
Query: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
PIDNAFVVNIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE MEPAKC+IN
Sbjct: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
Query: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++S
Sbjct: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339
BLAST of CsGy7G014220 vs. NCBI nr
Match:
KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 603 bits (1556), Expect = 2.21e-216
Identity = 285/337 (84.57%), Postives = 311/337 (92.28%), Query Frame = 0
Query: 4 GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
G KFVSNWE V+SVP YVYPPEKRPGN+VVPMA AIPV+DLS RDR LLVRKILDAS+
Sbjct: 2 GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQ 61
Query: 64 EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
EFGFFQ+INHGVS++V EET RIFK+FHAMSG EK KECS+DPN+SCRVYTSSENY KEQ
Sbjct: 62 EFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQ 121
Query: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183
HCWRDALIFNCHPL+KYVHFWPQ PPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+G
Sbjct: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRG 181
Query: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
Y GGEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 182 YLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
Query: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
PIDNAFVVNIGFVLQ+I+NGKL+AAEHR VTN+K SRQSLTYLVYPKDE MEPAKC+IN
Sbjct: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
Query: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340
EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++
Sbjct: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338
BLAST of CsGy7G014220 vs. ExPASy TrEMBL
Match:
A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)
HSP 1 Score: 699 bits (1804), Expect = 1.76e-254
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0
Query: 1 MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD
Sbjct: 1 MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
Query: 61 ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT 120
ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT
Sbjct: 61 ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT 120
Query: 121 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180
KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL
Sbjct: 121 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180
Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
Query: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC
Sbjct: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
BLAST of CsGy7G014220 vs. ExPASy TrEMBL
Match:
A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)
HSP 1 Score: 665 bits (1715), Expect = 6.51e-241
Identity = 324/341 (95.01%), Postives = 328/341 (96.19%), Query Frame = 0
Query: 1 MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
M QG AKFVSNWET ESVPESYVYPPEKRPGN+VVPMAKAIPVIDLSI DR LLVRKIL
Sbjct: 1 MQQGDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILG 60
Query: 61 ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT 120
ASKE GFFQIINHGVSK+VSE TMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENY
Sbjct: 61 ASKESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYK 120
Query: 121 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180
KEQ+HCWRDALIFNCHPLEKYVHFWPQ PPKYREVVGAYC+AMRKLVLEILELMSEGLGL
Sbjct: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGL 180
Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
GKGY GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
Query: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
GVQPID AFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEA MEPAKC
Sbjct: 241 GVQPIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKC 300
Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
BLAST of CsGy7G014220 vs. ExPASy TrEMBL
Match:
A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)
HSP 1 Score: 602 bits (1552), Expect = 4.19e-216
Identity = 286/338 (84.62%), Postives = 310/338 (91.72%), Query Frame = 0
Query: 4 GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
G KFVSNWE V+SVP Y+YPPEKRPGN+VVPMA AIPV+DLS RDR LLVRKILDAS+
Sbjct: 2 GEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDASQ 61
Query: 64 EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
EFGFFQ+INHGVS++V EET RIFK+FHAMSG EKAKECS+DP +SCRVYTSSENY KEQ
Sbjct: 62 EFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKEQ 121
Query: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183
HCWRDALIFNCHPLEKYVHFWPQ PPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+G
Sbjct: 122 THCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGRG 181
Query: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
Y EMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 182 YLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
Query: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
PIDNAFVVNIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE MEPAKC+IN
Sbjct: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
Query: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +N S
Sbjct: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSNCS 339
BLAST of CsGy7G014220 vs. ExPASy TrEMBL
Match:
A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)
HSP 1 Score: 598 bits (1543), Expect = 1.02e-214
Identity = 283/337 (83.98%), Postives = 309/337 (91.69%), Query Frame = 0
Query: 4 GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
G KFVSNWE V+SVP YVYPPEKRPGN+VVPMA AIPV+DLS DR LLVRKILDAS+
Sbjct: 2 GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQ 61
Query: 64 EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
EFGFFQ+INHGVS++V EET RIFK+FHAMSG EK KECS+DPN+SCRVYTSSENY KEQ
Sbjct: 62 EFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQ 121
Query: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183
HCWRDALIFNCHPL+KYVHFWPQ PPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+G
Sbjct: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRG 181
Query: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
Y GGEMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 182 YLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
Query: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
PIDNAFVVNIGFVLQ+I+NGKL+AAEHR VTN+K SRQSLTYLVYPKDE MEPAKC+IN
Sbjct: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
Query: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340
EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++
Sbjct: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338
BLAST of CsGy7G014220 vs. ExPASy TrEMBL
Match:
A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)
HSP 1 Score: 582 bits (1501), Expect = 2.56e-208
Identity = 272/334 (81.44%), Postives = 300/334 (89.82%), Query Frame = 0
Query: 8 FVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGF 67
FVSNW VESVPESYVYPPEKRPGN+ VP+ AIPV+DL+ DR LV KILDAS+EFGF
Sbjct: 7 FVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQEFGF 66
Query: 68 FQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCW 127
FQ+INHGVSK + EETMR+F+EFHAMSGPEK +ECSKDPNRSCRVYTSSEN+ KE+ HCW
Sbjct: 67 FQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCW 126
Query: 128 RDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGG 187
RDALIFNCHPL+KYVHFWPQ P YREVVGAYC+ MRKLV+EILEL+SEGLGLGKGYFG
Sbjct: 127 RDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGD 186
Query: 188 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDN 247
EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ +NGLQVLKDGQWIGV P+DN
Sbjct: 187 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHPVDN 246
Query: 248 AFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANP 307
AFVVNIGFVLQ+++NGKL+A EHRAV N+K SRQS+TYLVYPKD+A MEPAKCMINEANP
Sbjct: 247 AFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINEANP 306
Query: 308 PRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
Y SL FKDFQRNYLP A DTKAV+QYI ++ S
Sbjct: 307 SLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 340
BLAST of CsGy7G014220 vs. TAIR 10
Match:
AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 260.8 bits (665), Expect = 1.6e-69
Identity = 139/340 (40.88%), Postives = 205/340 (60.29%), Query Frame = 0
Query: 6 AKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEF 65
AK +S ++PE+YV P RP V + P+IDLS DR+ L+++I A F
Sbjct: 3 AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62
Query: 66 GFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVH 125
GFFQ+INHGV+K++ +E + + +EF +MS EK K S DP ++ R+ TS N KE+V+
Sbjct: 63 GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122
Query: 126 CWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 185
WRD L +C+P+ KYV+ WP PP ++E+V Y +R++ +I EL+SE LGL K Y
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182
Query: 186 GGEMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGV 245
+ E + VN+YPPCP P LT GL H DP+ +TIL QD V GLQ+L DGQW V
Sbjct: 183 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 242
Query: 246 QPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCM- 305
P +AFV+NIG LQ ++NG ++ HRAVTN++ R S+ + P D A+M PAK +
Sbjct: 243 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 302
Query: 306 --INEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETN 340
++ P Y+ + ++ + + R +D + ++ N
Sbjct: 303 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341
BLAST of CsGy7G014220 vs. TAIR 10
Match:
AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 226.9 bits (577), Expect = 2.5e-59
Identity = 126/325 (38.77%), Postives = 190/325 (58.46%), Query Frame = 0
Query: 15 VESVPESYVYPPEKRPG-NIVVPMAKAIPVIDLSI---RDRTLLVRKILDASKEFGFFQI 74
V+ VP +YV P RP + V +IP+IDL +R ++ + A GFFQI
Sbjct: 15 VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74
Query: 75 INHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCWRDA 134
NHGV ++ ++ M +EF S E+ K S D ++ R+ TS N +KE+V WRD
Sbjct: 75 KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134
Query: 135 LIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGGEMS 194
L +C+P+E +++ WP P +REV Y ++R LVL +LE +SE LGL K +
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194
Query: 195 EN-PLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPIDNA 254
++ + +N+YP CP P LT GL H D +LIT+L QD V+GLQV KDG+WI V P+ N
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254
Query: 255 FVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA--N 314
F+VN+G +Q+I+N K ++ HRAV NS R S+ P ++A++ PA+ +INE +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314
Query: 315 PPRYRSLNFKDFQRNYLPRAVDTKA 332
P YR+ + ++ + A DT++
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTES 338
BLAST of CsGy7G014220 vs. TAIR 10
Match:
AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 220.3 bits (560), Expect = 2.4e-57
Identity = 123/334 (36.83%), Postives = 191/334 (57.19%), Query Frame = 0
Query: 9 VSNWETVESVPESYVYPPEKRPG-NIVVPMAKAIPVIDLSIRD-----RTLLVRKILDAS 68
VS++ + +P +YV P RP + V +IP+IDL RD R ++V+++ A
Sbjct: 11 VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDL--RDLHGPNRAVIVQQLASAC 70
Query: 69 KEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKE 128
+GFFQI NHGV + + +EF E+ K S DP ++ R+ TS N +
Sbjct: 71 STYGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGAD 130
Query: 129 QVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGK 188
+V WRD L +C P+E ++ WP P +REV Y ++R LVL +LE +SE LGL
Sbjct: 131 KVLNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLES 190
Query: 189 GYFGGEMSENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWI 248
+ + ++ + N+YPPCP P LT GL H DP++IT+L QD V+GLQV KD +W+
Sbjct: 191 DHISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWV 250
Query: 249 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 308
V PI N F+VNIG +Q+I+N K ++ HRAV N++ R S+ +P +A++ PA
Sbjct: 251 AVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHE 310
Query: 309 MINEANP-PRYRSLNFKDFQRNYLPRAVDTKAVM 334
++NE + YR+ F ++ + R++ T + +
Sbjct: 311 LVNEQDSLAIYRTYPFVEYWDKFWNRSLATASCL 341
BLAST of CsGy7G014220 vs. TAIR 10
Match:
AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 197.6 bits (501), Expect = 1.6e-50
Identity = 113/317 (35.65%), Postives = 171/317 (53.94%), Query Frame = 0
Query: 15 VESVPESYVYPPEKRP--GNIVVPMAKAIPVIDLSIRD----RTLLVRKILDASKEFGFF 74
V VP+ YV PP +RP G+ + +PVIDLS+ R+L + +I A KEFGFF
Sbjct: 24 VPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGFF 83
Query: 75 QIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCWR 134
Q+INHG+ V + + +F + EK S + + R Y +S N++ ++VH WR
Sbjct: 84 QVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYWR 143
Query: 135 DALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGGE 194
D + HPL K++ WP PP Y++ VG Y A L +++E +SE LGL K Y E
Sbjct: 144 DFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQEE 203
Query: 195 MSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLK-DGQWIGVQPID 254
+ E + ++ VN YP CP P + LG+ H D S +TIL Q GLQ++ + W+ V I+
Sbjct: 204 IEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYIE 263
Query: 255 NAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEAN 314
A +V +G +++++NG ++ HR N + R S L + PA ++N N
Sbjct: 264 GALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPNN 323
Query: 315 PPRYRSLNFKDFQRNYL 324
P Y +F DF NY+
Sbjct: 324 APAYGEFSFNDF-LNYI 338
BLAST of CsGy7G014220 vs. TAIR 10
Match:
AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 189.5 bits (480), Expect = 4.5e-48
Identity = 118/327 (36.09%), Postives = 170/327 (51.99%), Query Frame = 0
Query: 20 ESYVYPPEKRPGNIVVP-----MAKAIPVIDLSI-----RDRTLLVRKILDASKEFGFFQ 79
E+++ PE RP + + IP IDLS D+T + ++I +A K +GFFQ
Sbjct: 6 EAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQ 65
Query: 80 IINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCWR- 139
+INHG+ + + EF ++ EK K + N + E +TK V W+
Sbjct: 66 VINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVN---PMGYHDEEHTK-NVRDWKE 125
Query: 140 -------DALIFNCHP------LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMS 199
D+ I P L K + WPQ P +REV Y + KL +LEL+S
Sbjct: 126 IFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVS 185
Query: 200 EGLGL-GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQV 259
LGL G G + L NHYPPCPNP L LG+ +H D +T+L QD V GLQV
Sbjct: 186 ISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQV 245
Query: 260 LK--DGQWIGVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKD 319
+ DGQWI V+PI +A ++N+G +Q+ TN + +AEHR V N+ R S+ + +P
Sbjct: 246 SRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSH 305
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| P24397 | 1.1e-70 | 43.47 | Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1 | [more] |
| Q9FLV0 | 2.2e-68 | 40.88 | Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... | [more] |
| Q8W2X5 | 5.1e-65 | 39.26 | Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... | [more] |
| Q40062 | 1.2e-58 | 40.71 | 2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=... | [more] |
| Q9ZSA7 | 3.5e-58 | 38.77 | Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_004146985.1 | 3.64e-254 | 100.00 | hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44748.1 hypothetical protein Csa... | [more] |
| XP_008451261.1 | 1.34e-240 | 95.01 | PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo] | [more] |
| XP_038897058.1 | 6.31e-232 | 91.94 | hyoscyamine 6-dioxygenase-like [Benincasa hispida] | [more] |
| XP_023514953.1 | 2.59e-217 | 84.91 | hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo] | [more] |
| KAG6593078.1 | 2.21e-216 | 84.57 | Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0K5H3 | 1.76e-254 | 100.00 | Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G... | [more] |
| A0A1S3BR20 | 6.51e-241 | 95.01 | hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1 | [more] |
| A0A6J1KW36 | 4.19e-216 | 84.62 | hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 ... | [more] |
| A0A6J1H5H6 | 1.02e-214 | 83.98 | hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=... | [more] |
| A0A6J1CP90 | 2.56e-208 | 81.44 | hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT5G24530.1 | 1.6e-69 | 40.88 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT4G10490.1 | 2.5e-59 | 38.77 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT4G10500.1 | 2.4e-57 | 36.83 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT2G44800.1 | 1.6e-50 | 35.65 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT3G19000.1 | 4.5e-48 | 36.09 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
