CsGy7G002160 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy7G002160 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 974.2 bits (2517), Expect = 8.9e-283
Identity = 494/776 (63.66%), Postives = 598/776 (77.06%), Query Frame = 0

Query: 1   MKLSTLSSSILLLSFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADV---VANSHHE 60
           MKL T + S LLL  L+  SS     S    SYVVY GAHSH G+   D    V  +H++
Sbjct: 1   MKL-THNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYD 60

Query: 61  FLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTT 120
           FL  F  S E  T D IFYSYT+HINGFAA L+ ++A +++KHP+VVSVF N+  KLHTT
Sbjct: 61  FLGSFTGSRERAT-DAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTT 120

Query: 121 RSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKG 180
           RSW+F+GLE+ N  + S SIW+KARFGEDTII NLDTGVW ESKSF D+  GPIP RWKG
Sbjct: 121 RSWDFLGLEH-NSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKG 180

Query: 181 ICQNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVA 240
           ICQNQKD +FHCNRKLIGARYFNKGYA+ VG LNSSF SPRD +GHGSHTLSTA G+FV 
Sbjct: 181 ICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVP 240

Query: 241 GASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSL 300
           G S+FG G GTAKGGSPRARVAAYKVCWPP  GNEC+DAD+LAAFD AIHDG DV+SVSL
Sbjct: 241 GVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSL 300

Query: 301 GGDPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFL 360
           GG+P + FNDSVAIGSFHA K  IVV+CSAGNSGPA  TV+NVAPWQITVGASTMDR+F 
Sbjct: 301 GGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFA 360

Query: 361 SLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKG 420
           S +VLGN K  +G+SLS  ALP  K YP+M + + +  NAS  +AQLCK G+L+P+K KG
Sbjct: 361 SNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKG 420

Query: 421 KILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVF 480
           KILVCLRG N RV+KG    L G  GM+L N  ++GN++LADPHVLPA+ +   D  AV 
Sbjct: 421 KILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVS 480

Query: 481 AYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYT 540
            YI+ TK P A+ITP+ T LG++PAP MA+FSS GP+ V P+ILKPDITAPG+SVIAAYT
Sbjct: 481 RYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYT 540

Query: 541 EAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASIL 600
            A  PTN++FD RR+ FN++SGTSMSCPH+SGIAGLLKT YP WSPAAI+SAIMTTA+I+
Sbjct: 541 GAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIM 600

Query: 601 DNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQ 660
           D+   P+ NA+   A+PF++GAGHV PN A +PGLVYD+ + +YL+FLC+LGYN +QIS 
Sbjct: 601 DDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISV 660

Query: 661 FSNGPFNCSDP-ISPTNLNYPSITVPKLSRS-ITITRRLKNVGSPGTYKAEIRKPAGISV 720
           FS   F CS P IS  NLNYPSITVP L+ S +T++R +KNVG P  Y  ++  P G+ V
Sbjct: 661 FSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYV 720

Query: 721 WVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKV 772
            VKP  L+FT++GE+ +FKV++ VK +    K YV+G+L+WSD KH VRSPIVVK+
Sbjct: 721 AVKPTSLNFTKVGEQKTFKVIL-VKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of CsGy7G002160 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 4.9e-265
Identity = 457/765 (59.74%), Postives = 576/765 (75.29%), Query Frame = 0

Query: 12  LLSFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPA---DVVANSHHEFLQPFLKSGEE 71
           L S LLL +  F+P  A+K+SY+VY+G+H+H  Q  +   D VA+SH  FL  F+ S E 
Sbjct: 21  LSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN 80

Query: 72  FTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRSWEFMGLENK 131
             K+ IFYSY RHINGFAA+L++  A ++AKHP VVSVF N+GRKLHTT SW FM L  K
Sbjct: 81  -AKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFM-LLAK 140

Query: 132 NGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGICQNQKDPSFH 191
           NGV++  S+W KA +GEDTII NLDTGVW ESKSFSD+ YG +P RWKG C         
Sbjct: 141 NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHK----DVP 200

Query: 192 CNRKLIGARYFNKGYASVVG-PLNSSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKG 251
           CNRKLIGARYFNKGY +  G P N+S+ + RD +GHGSHTLSTA GNFV GA+VFG+G G
Sbjct: 201 CNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNG 260

Query: 252 TAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGGDPNALFND 311
           TA GGSP+ARVAAYKVCWPP  G ECFDADILAA + AI DGVDVLS S+GGD     +D
Sbjct: 261 TASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSD 320

Query: 312 SVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSLVVLGNRKQ 371
            +AIGSFHA+K+G+ V+CSAGNSGP +GTV+NVAPW ITVGAS+MDR+F + V L N + 
Sbjct: 321 GIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQS 380

Query: 372 IEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKILVCLRGDN 431
            +G SLS+  LP +K+Y L++AAD  +AN +V +A LCK G+L+P K KGKILVCLRGDN
Sbjct: 381 FKGTSLSK-PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDN 440

Query: 432 ARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAYINSTKYPE 491
           ARVDKG QA  AGAAGM+L N++ SGNEI++D HVLPAS I++ DG  +F+Y++STK P+
Sbjct: 441 ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPK 500

Query: 492 AYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAEGPTNQEF 551
            YI   T  L  +PAPFMA+FSS GPNT+TP ILKPDITAPG+++IAA+TEA GPT+ + 
Sbjct: 501 GYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDS 560

Query: 552 DNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDNNFEPLLNA 611
           DNRR PFN+ SGTSMSCPH+SG+ GLLKTL+PHWSPAAI+SAIMTT+   +N  +P+++ 
Sbjct: 561 DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDE 620

Query: 612 SYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFSNGP-FNCS 671
           S+  A+PF+YG+GHV PN AA PGLVYD+   +YL FLCA+GYN   +  F+  P + C 
Sbjct: 621 SFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCR 680

Query: 672 DPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKPKKLSFTR 731
              +  + NYPSITVP L+ SIT+TR+LKNVG P TY A  R+P G+ V V+PK+L+F +
Sbjct: 681 QGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNK 740

Query: 732 LGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKV 772
            GE   F+  M ++   V    YV+G+L W+D  H+VRSPIVV++
Sbjct: 741 TGEVKIFQ--MTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of CsGy7G002160 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 704.9 bits (1818), Expect = 1.0e-201
Identity = 396/795 (49.81%), Postives = 511/795 (64.28%), Query Frame = 0

Query: 6   LSSSILLLSFLLLSSSLFT----PTSAIKRSYVVYMGAHSHGGQKPA---DVVANSHHEF 65
           + SSI  L  +L S  LFT      +  K+ Y+VYMGAHSHG    +   ++  +SH++ 
Sbjct: 1   MGSSIFCLHLILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDL 60

Query: 66  LQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTR 125
           L     S E+  K+ I YSY RHINGFAA+LE+E A  +AK+P VVSVFL++  KLHTTR
Sbjct: 61  LGSIFGSREK-AKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTR 120

Query: 126 SWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWK-G 185
           SWEF+GL  +       S W+K RFGE+TIIGN+DTGVW ES+SFSD  YG +P +W+ G
Sbjct: 121 SWEFLGLHRR----GQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGG 180

Query: 186 ICQNQKDPSF---HCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGN 245
           +CQ  K P      CNRKLIGARY+NK + +  G L+   H+ RD  GHG+HTLSTAGGN
Sbjct: 181 LCQINKLPGSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGN 240

Query: 246 FVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLS 305
           FV GA VF +G GTAKGGSPRARVAAYKVCW       C+ AD+LAA D AI DGVDV++
Sbjct: 241 FVPGARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVIN 300

Query: 306 VSLGGD----PNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGAS 365
           VS G         +F D ++IG+FHAI   I+++ SAGN GP  GTV NVAPW  T+ AS
Sbjct: 301 VSFGVSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAAS 360

Query: 366 TMDRKFLSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTL 425
           T+DR F S + + N + IEG SL  + LP  + + L+ + D +LANA+  +AQLC+ GTL
Sbjct: 361 TLDRDFSSNLTI-NNQLIEGASLFVN-LPPNQAFSLILSTDAKLANATFRDAQLCRRGTL 420

Query: 426 NPMKAKGKILVCLR-GDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVL-----P 485
           +  K  GKI++C R G    V +G +AL AGA GMIL N   +G  + A+PHV      P
Sbjct: 421 DRTKVNGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTP 480

Query: 486 ASHINFTDGSAVFAYINSTKYP-----EAYITPATTQLGIRPAPFMAAFSSVGPNTVTPE 545
                          I     P        ++ A T  G +PAP MA+FSS GPN + P 
Sbjct: 481 PRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPS 540

Query: 546 ILKPDITAPGLSVIAAYTEAEGPTNQEFDNRR-IPFNSVSGTSMSCPHVSGIAGLLKTLY 605
           ILKPD+TAPG++++AAY+E    ++   DNRR   FN + GTSMSCPH SGIAGLLKT +
Sbjct: 541 ILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRH 600

Query: 606 PHWSPAAIKSAIMTTASILDNNFEPLLNA-SYSVASPFNYGAGHVHPNGAADPGLVYDIE 665
           P WSPAAIKSAIMTTA+ LDN   P+ +A   ++A  F YG+GHV P+ A +PGLVYD+ 
Sbjct: 601 PSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLS 660

Query: 666 VNEYLSFLCALGYNKAQISQFS-NGPFNCSDPISPTNLNYPSITVPKLS-RSITITRRLK 725
           + +YL+FLCA GY++  IS  + N  F CS   S  +LNYPSIT+P L  + +TI R + 
Sbjct: 661 LTDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVT 720

Query: 726 NVGSPGTYKAEIRKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLI 771
           NVG P TY    R P G S+ V P  L+FT++GE  +FKV+++       +K Y +GDL 
Sbjct: 721 NVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRK-YEFGDLR 780

BLAST of CsGy7G002160 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.7e-169
Identity = 349/792 (44.07%), Postives = 467/792 (58.96%), Query Frame = 0

Query: 16  LLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIF 75
           LL    L    +  K+ Y+VY G H   G K    +   HH +LQ  +K  EE  +  + 
Sbjct: 10  LLFLIPLLASCAEEKQVYIVYFGEHK--GDKAFHEIEEHHHSYLQS-VKESEEDARASLL 69

Query: 76  YSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRK--LHTTRSWEFMGLE------- 135
           YSY   INGFAA L  + A +L K  +VVSVF +  RK   HTTRSWEF+GLE       
Sbjct: 70  YSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSD 129

Query: 136 ---NKNGVIN----SESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGIC 195
               KN   +      +  KKA+ G+  I+G LD+GVW ESKSF+D   GP+P  WKGIC
Sbjct: 130 VPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGIC 189

Query: 196 Q-NQKDPSFHCNRKLIGARYFNKGYASVVGPLNSS----FHSPRDKEGHGSHTLSTAGGN 255
           Q      S HCNRK+IGARY+ KGY    G  N++    F SPRD +GHGSHT STA G 
Sbjct: 190 QTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGR 249

Query: 256 FVAGASVF-GLGKGTAKGGSPRARVAAYKVCW-PPKA----GNECFDADILAAFDFAIHD 315
            V GAS   G  KG+A GG+P AR+A YK CW  P A    GN C + D+LAA D AI D
Sbjct: 250 RVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIAD 309

Query: 316 GVDVLSVSLG-GDPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITV 375
           GV V+S+S+G  +P     D +A+G+ HA+K  IVV  SAGNSGP  GT++N+APW ITV
Sbjct: 310 GVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITV 369

Query: 376 GASTMDRKFLSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKA 435
           GAST+DR F+  +VLGN   I+ +S++  A    K  PL+ A++V +   +++E   C  
Sbjct: 370 GASTLDRAFVGGLVLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNETSQCLP 429

Query: 436 GTLNPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASH 495
            +L P    GK+++CLRG  +R+ KG +   AG AGMIL N   +GNE+ +D H +P + 
Sbjct: 430 NSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAG 489

Query: 496 INFTDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITA 555
           +  T    +  YI + K P+A+I P  T    + AP M  FSS GPN V P ILKPDITA
Sbjct: 490 VTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITA 549

Query: 556 PGLSVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIK 615
           PGL ++AA++ A+ P+    D R   +N  SGTSMSCPHV+G   LLK ++P WS AAI+
Sbjct: 550 PGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIR 609

Query: 616 SAIMTTASILDNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCA 675
           SA+MTTA + ++  +P+ + +   A+PF  G+GH  P  AADPGLVYD     YL + C+
Sbjct: 610 SALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYLLYGCS 669

Query: 676 LGYNKAQISQFSNGPFNCSDPISP-TNLNYPSITVPKLSRSITITRRLKNVG---SPGTY 735
           +           +  F C   I P  N NYPSI VP L +++T+ R + NVG   S  TY
Sbjct: 670 VNITNI------DPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTY 729

Query: 736 KAEIRKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKV----AKKNYVYGDLIWSDG 772
              ++ P+GISV   P  LSF R+G++  FK+++K  + +V     K  Y +G   W+D 
Sbjct: 730 LFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDK 789

BLAST of CsGy7G002160 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 5.0e-169
Identity = 351/784 (44.77%), Postives = 486/784 (61.99%), Query Frame = 0

Query: 1   MKLSTLSSS----ILLLSFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPA--DVVANS 60
           M  S LSS+    +L L F  +SSS     S+ + +Y+V+M       Q P+  D+ +N 
Sbjct: 1   MSSSFLSSTAFFLLLCLGFCHVSSS-----SSDQGTYIVHMAK----SQMPSSFDLHSNW 60

Query: 61  HHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKL 120
           +   L+    S E      + Y+Y   I+GF+  L  E A  L   P V+SV      +L
Sbjct: 61  YDSSLRSISDSAE------LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYEL 120

Query: 121 HTTRSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHR 180
           HTTR+  F+GL+      ++  ++ +A    D ++G LDTGVW ESKS+SD+ +GPIP  
Sbjct: 121 HTTRTPLFLGLDE-----HTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSS 180

Query: 181 WKGICQ-NQKDPSFHCNRKLIGARYFNKGYASVVGPLNSS--FHSPRDKEGHGSHTLSTA 240
           WKG C+      +  CNRKLIGAR+F +GY S +GP++ S    SPRD +GHG+HT STA
Sbjct: 181 WKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTA 240

Query: 241 GGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVD 300
            G+ V GAS+ G   GTA+G +PRARVA YKVCW       CF +DILAA D AI D V+
Sbjct: 241 AGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVN 300

Query: 301 VLSVSLGGDPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGAST 360
           VLS+SLGG  +  + D VAIG+F A++ GI+V CSAGN+GP++ +++NVAPW  TVGA T
Sbjct: 301 VLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGT 360

Query: 361 MDRKFLSLVVLGNRKQIEGESLSQ-DALPSKKLYPLMNAADVRLANASVHEAQLCKAGTL 420
           +DR F +L +LGN K   G SL + +ALP  KL P + A +   +NA+     LC  GTL
Sbjct: 361 LDRDFPALAILGNGKNFTGVSLFKGEALPD-KLLPFIYAGNA--SNAT--NGNLCMTGTL 420

Query: 421 NPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINF 480
            P K KGKI++C RG NARV KG+    AG  GMILAN   +G E++AD H+LPA+ +  
Sbjct: 421 IPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGE 480

Query: 481 TDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGL 540
             G  +  Y+ +   P A I+   T +G++P+P +AAFSS GPN++TP ILKPD+ APG+
Sbjct: 481 KAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGV 540

Query: 541 SVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAI 600
           +++AA+T A GPT    D+RR+ FN +SGTSMSCPHVSG+A LLK+++P WSPAAI+SA+
Sbjct: 541 NILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSAL 600

Query: 601 MTTASILDNNFEPLLN-ASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALG 660
           MTTA     + +PLL+ A+   ++PF++GAGHV P  A +PGL+YD+   +YL FLCAL 
Sbjct: 601 MTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALN 660

Query: 661 YNKAQISQFSNGPFNC--SDPISPTNLNYPSITVPKLS-RSITITRRLKNVGSPGTYKAE 720
           Y   QI   S   + C  S   S  +LNYPS  V      +   TR + +VG  GTY  +
Sbjct: 661 YTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVK 720

Query: 721 I-RKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRS 770
           +  +  G+ + V+P  L+F    E+ S+ V   V   K +  N  +G + WSDGKH V S
Sbjct: 721 VTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGS 754

BLAST of CsGy7G002160 vs. NCBI nr
Match: XP_011658647.1 (subtilisin-like protease SBT5.3 [Cucumis sativus] >KAE8645706.1 hypothetical protein Csa_020514 [Cucumis sativus])

HSP 1 Score: 1541 bits (3991), Expect = 0.0
Identity = 770/772 (99.74%), Postives = 770/772 (99.74%), Query Frame = 0

Query: 1   MKLSTLSSSILLLSFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFLQ 60
           MKLSTLSSSILLLSFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFLQ
Sbjct: 1   MKLSTLSSSILLLSFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFLQ 60

Query: 61  PFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRSW 120
           PFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRSW
Sbjct: 61  PFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRSW 120

Query: 121 EFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGICQ 180
           EFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGICQ
Sbjct: 121 EFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGICQ 180

Query: 181 NQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGAS 240
           NQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGAS
Sbjct: 181 NQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGAS 240

Query: 241 VFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGGD 300
           VFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGGD
Sbjct: 241 VFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGGD 300

Query: 301 PNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSLV 360
           PN LFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKF SLV
Sbjct: 301 PNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSLV 360

Query: 361 VLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKIL 420
           VLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKIL
Sbjct: 361 VLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKIL 420

Query: 421 VCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAYI 480
           VCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAYI
Sbjct: 421 VCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAYI 480

Query: 481 NSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAE 540
           NSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAE
Sbjct: 481 NSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAE 540

Query: 541 GPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDNN 600
           GPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDNN
Sbjct: 541 GPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDNN 600

Query: 601 FEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFSN 660
           FEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFSN
Sbjct: 601 FEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFSN 660

Query: 661 GPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKPK 720
           GPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKPK
Sbjct: 661 GPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKPK 720

Query: 721 KLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 772
           KLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV
Sbjct: 721 KLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 772

BLAST of CsGy7G002160 vs. NCBI nr
Match: TYK03381.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1475 bits (3819), Expect = 0.0
Identity = 734/773 (94.95%), Postives = 755/773 (97.67%), Query Frame = 0

Query: 1   MKLSTLSSSILLLS-FLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFL 60
           MKLSTLSSSI LLS FLLLSSSLFTPTSAIKRSYVVYMGAHSHGG+KPADVVA+SHH+FL
Sbjct: 1   MKLSTLSSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 60

Query: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRS 120
           QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVA QLAKHP+VVSVFLNRGRKLHTTRS
Sbjct: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 120

Query: 121 WEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGIC 180
           WEFMGLEN+NGVI SESIWKKARFGEDTIIGNLDTGVWAESKSFSDDE GPIPHRWKGIC
Sbjct: 121 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 180

Query: 181 QNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240
           QNQ DPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA
Sbjct: 181 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240

Query: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGG 300
           SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFD AIHDGVDVLSVSLGG
Sbjct: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 300

Query: 301 DPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSL 360
           DPN LFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKF SL
Sbjct: 301 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 360

Query: 361 VVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKI 420
           VVLGN+KQIEGESLSQ+ALPSKKLYPLMNAADVRLANAS HEAQLCKAGTL+PMKAKGKI
Sbjct: 361 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 420

Query: 421 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAY 480
           LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPA HIN+TDGSAVFAY
Sbjct: 421 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 480

Query: 481 INSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 540
           INSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA
Sbjct: 481 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 540

Query: 541 EGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDN 600
           EGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL+TLYPHWSPAAIKSAIMTTAS LDN
Sbjct: 541 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 600

Query: 601 NFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFS 660
           NFEPLLNASY VA+PFNYGAGHVHPNGA DPGLVYDI+VNEYLSFLCALGY+KAQISQFS
Sbjct: 601 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 660

Query: 661 NGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKP 720
           +GPFNCS+PIS TNLNYPSITVPKLSRSITITRRLKNVGSPGTY+A+IRKPAGISVWVKP
Sbjct: 661 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 720

Query: 721 KKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 772
           KKL+FT+LGEELSFKV MKVK  KVAKKNYVYGDLIWSDGKHHVRSPIVVKVV
Sbjct: 721 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of CsGy7G002160 vs. NCBI nr
Match: XP_008462019.1 (PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo])

HSP 1 Score: 1474 bits (3816), Expect = 0.0
Identity = 733/773 (94.83%), Postives = 755/773 (97.67%), Query Frame = 0

Query: 1   MKLSTLSSSILLLS-FLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFL 60
           M+LSTLSSSI LLS FLLLSSSLFTPTSAIKRSYVVYMGAHSHGG+KPADVVA+SHH+FL
Sbjct: 1   MRLSTLSSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 60

Query: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRS 120
           QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVA QLAKHP+VVSVFLNRGRKLHTTRS
Sbjct: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 120

Query: 121 WEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGIC 180
           WEFMGLEN+NGVI SESIWKKARFGEDTIIGNLDTGVWAESKSFSDDE GPIPHRWKGIC
Sbjct: 121 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 180

Query: 181 QNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240
           QNQ DPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA
Sbjct: 181 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240

Query: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGG 300
           SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFD AIHDGVDVLSVSLGG
Sbjct: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 300

Query: 301 DPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSL 360
           DPN LFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKF SL
Sbjct: 301 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 360

Query: 361 VVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKI 420
           VVLGN+KQIEGESLSQ+ALPSKKLYPLMNAADVRLANAS HEAQLCKAGTL+PMKAKGKI
Sbjct: 361 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 420

Query: 421 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAY 480
           LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPA HIN+TDGSAVFAY
Sbjct: 421 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 480

Query: 481 INSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 540
           INSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA
Sbjct: 481 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 540

Query: 541 EGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDN 600
           EGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL+TLYPHWSPAAIKSAIMTTAS LDN
Sbjct: 541 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 600

Query: 601 NFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFS 660
           NFEPLLNASY VA+PFNYGAGHVHPNGA DPGLVYDI+VNEYLSFLCALGY+KAQISQFS
Sbjct: 601 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 660

Query: 661 NGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKP 720
           +GPFNCS+PIS TNLNYPSITVPKLSRSITITRRLKNVGSPGTY+A+IRKPAGISVWVKP
Sbjct: 661 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 720

Query: 721 KKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 772
           KKL+FT+LGEELSFKV MKVK  KVAKKNYVYGDLIWSDGKHHVRSPIVVKVV
Sbjct: 721 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of CsGy7G002160 vs. NCBI nr
Match: XP_038888965.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 1406 bits (3639), Expect = 0.0
Identity = 691/768 (89.97%), Postives = 733/768 (95.44%), Query Frame = 0

Query: 5   TLSSSILLLSFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFLQPFLK 64
           T+SS+  LL FLLLSSSL TPTSAIKRSYVVYMGAHSHGG+KPADVVA+SHH+FL+PFLK
Sbjct: 5   TISSATFLLPFLLLSSSLLTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHKFLRPFLK 64

Query: 65  SGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRSWEFMG 124
             ++FT+DVIFYSYTRHINGFAAMLEDEVA QLAKHPKVVS+FLN+GRKLHTTRSWEFMG
Sbjct: 65  R-DDFTEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSIFLNKGRKLHTTRSWEFMG 124

Query: 125 LENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGICQNQKD 184
           +EN NGVI SESIWKKARFGEDTIIGNLDTGVW ESKSFSDD+ GPIP RW+GICQNQ D
Sbjct: 125 MENNNGVITSESIWKKARFGEDTIIGNLDTGVWPESKSFSDDDLGPIPLRWRGICQNQND 184

Query: 185 PSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGL 244
           PSFHCNRKLIGARYFNKGY SVVGPLNSSF+SPRDKEGHGSHTLSTAGGNFVAGASVFGL
Sbjct: 185 PSFHCNRKLIGARYFNKGYESVVGPLNSSFNSPRDKEGHGSHTLSTAGGNFVAGASVFGL 244

Query: 245 GKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGGDPNAL 304
           G GTAKGGSPRARVAAYKVCWPP AGNECFDADILAAFD AIHDGVDVLSVSLGGDPN L
Sbjct: 245 GNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDSAIHDGVDVLSVSLGGDPNPL 304

Query: 305 FNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSLVVLGN 364
           FNDSVAIGSFHA+KHGIVVICSAGNSGPAAGTVTN+APWQITVGASTMDRKF SLVVLGN
Sbjct: 305 FNDSVAIGSFHAVKHGIVVICSAGNSGPAAGTVTNIAPWQITVGASTMDRKFPSLVVLGN 364

Query: 365 RKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKILVCLR 424
           +KQIEGESLSQ+ALPS+K YPL++AADVRLANAS HEAQLCKAGTL+PMKAKGKILVCLR
Sbjct: 365 KKQIEGESLSQNALPSRKFYPLVSAADVRLANASAHEAQLCKAGTLDPMKAKGKILVCLR 424

Query: 425 GDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAYINSTK 484
           GDNARVDKGEQALLAGA GMILANNE SGNEILADPHVLPASHINFTDG+AV  YINSTK
Sbjct: 425 GDNARVDKGEQALLAGAVGMILANNEPSGNEILADPHVLPASHINFTDGTAVLTYINSTK 484

Query: 485 YPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAEGPTN 544
           +P+AYIT ATTQLGIRPAPFMAAFSSVGPNT+ PEILKPD+TAPGLSVIAAYTEAEGPTN
Sbjct: 485 FPKAYITRATTQLGIRPAPFMAAFSSVGPNTIIPEILKPDVTAPGLSVIAAYTEAEGPTN 544

Query: 545 QEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDNNFEPL 604
           QE+DNRR+PFNSVSGTSMSCPHVSGIAGLL+TLYPHWSPAAIKSAIMTTA+ LDNNFEPL
Sbjct: 545 QEYDNRRLPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTANTLDNNFEPL 604

Query: 605 LNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFSNGPFN 664
           LNASYSVA+P NYGAGHVHPNGA DPGL+YDIEVNEYL FLCALGYN+AQISQFS+GPFN
Sbjct: 605 LNASYSVATPLNYGAGHVHPNGATDPGLIYDIEVNEYLLFLCALGYNQAQISQFSSGPFN 664

Query: 665 CSDPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKPKKLSF 724
           CS+PI+ TNLNYPSITVP+LSRSITITRRLKNVGSPGTYKA+IRKPAGISVWVKPKKL+F
Sbjct: 665 CSEPINLTNLNYPSITVPELSRSITITRRLKNVGSPGTYKAQIRKPAGISVWVKPKKLNF 724

Query: 725 TRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 772
           TRLGEE SFKVLMKVKE+  AKKNYVYGDLIWSDGKHHVRSPIVVKVV
Sbjct: 725 TRLGEEQSFKVLMKVKEQNAAKKNYVYGDLIWSDGKHHVRSPIVVKVV 771

BLAST of CsGy7G002160 vs. NCBI nr
Match: KAA0059266.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1404 bits (3635), Expect = 0.0
Identity = 707/775 (91.23%), Postives = 734/775 (94.71%), Query Frame = 0

Query: 1   MKLSTLSSSILLLS-FLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFL 60
           MKLSTLSSSI LLS FLLLSSSLFTPTSAIKRSYVVYMGAHSHGG+KPADVVA+SHH+FL
Sbjct: 1   MKLSTLSSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 60

Query: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRS 120
           QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVA QLAKHP+VVSVFLNRGRKLHTTRS
Sbjct: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 120

Query: 121 WEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGIC 180
           WEFMGLEN+NGVI SESIWKKARFGEDTIIGNLDTGVWAESKSFSDDE GPIPHRWKGIC
Sbjct: 121 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 180

Query: 181 QNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240
           QNQ DPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA
Sbjct: 181 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240

Query: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGG 300
           SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFD AIHDGVDVLSVSLGG
Sbjct: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 300

Query: 301 DPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSL 360
           DPN LFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKF SL
Sbjct: 301 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 360

Query: 361 VVLGNRKQIE--GESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKG 420
           VVLGN+KQIE  G  +    L  +     +N   + L + +   +QLCKAGTL+PMKAKG
Sbjct: 361 VVLGNKKQIEETGTIIFCSLLAIEYTAYYLNYQYLTLTSWTPF-SQLCKAGTLDPMKAKG 420

Query: 421 KILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVF 480
           KILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPA HIN+TDGSAVF
Sbjct: 421 KILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVF 480

Query: 481 AYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYT 540
           AYINSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYT
Sbjct: 481 AYINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYT 540

Query: 541 EAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASIL 600
           EAEGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL+TLYPHWSPAAIKSAIMTTAS L
Sbjct: 541 EAEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTL 600

Query: 601 DNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQ 660
           DNNFEPLLNASY VA+PFNYGAGHVHPNGA DPGLVYDI+VNEYLSFLCALGY+KAQISQ
Sbjct: 601 DNNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQ 660

Query: 661 FSNGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWV 720
           FS+GPFNCS+PIS TNLNYPSITVPKLSRSITITRRLKNVGSPGTY+A+IRKPAGISVWV
Sbjct: 661 FSDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWV 720

Query: 721 KPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 772
           KPKKL+FT+LGEELSFKV MKVK  KVAKKNYVYGDLIWSDGKHHVRSPIVVKVV
Sbjct: 721 KPKKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 774

BLAST of CsGy7G002160 vs. ExPASy TrEMBL
Match: A0A0A0K313 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G030520 PE=3 SV=1)

HSP 1 Score: 1484 bits (3843), Expect = 0.0
Identity = 734/736 (99.73%), Postives = 734/736 (99.73%), Query Frame = 0

Query: 37  MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQ 96
           MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQ
Sbjct: 1   MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQ 60

Query: 97  LAKHPKVVSVFLNRGRKLHTTRSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGV 156
           LAKHPKVVSVFLNRGRKLHTTRSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGV
Sbjct: 61  LAKHPKVVSVFLNRGRKLHTTRSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGV 120

Query: 157 WAESKSFSDDEYGPIPHRWKGICQNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 216
           WAESKSFSDDEYGPIPHRWKGICQNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS
Sbjct: 121 WAESKSFSDDEYGPIPHRWKGICQNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180

Query: 217 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA 276
           PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA
Sbjct: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA 240

Query: 277 DILAAFDFAIHDGVDVLSVSLGGDPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 336
           DILAAFDFAIHDGVDVLSVSLGGDPN LFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT
Sbjct: 241 DILAAFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300

Query: 337 VTNVAPWQITVGASTMDRKFLSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 396
           VTNVAPWQITVGASTMDRKF SLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN
Sbjct: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 360

Query: 397 ASVHEAQLCKAGTLNPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 456
           ASVHEAQLCKAGTLNPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI
Sbjct: 361 ASVHEAQLCKAGTLNPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 420

Query: 457 LADPHVLPASHINFTDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 516
           LADPHVLPASHINFTDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV
Sbjct: 421 LADPHVLPASHINFTDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 480

Query: 517 TPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 576
           TPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT
Sbjct: 481 TPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 540

Query: 577 LYPHWSPAAIKSAIMTTASILDNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDI 636
           LYPHWSPAAIKSAIMTTASILDNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDI
Sbjct: 541 LYPHWSPAAIKSAIMTTASILDNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDI 600

Query: 637 EVNEYLSFLCALGYNKAQISQFSNGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKN 696
           EVNEYLSFLCALGYNKAQISQFSNGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKN
Sbjct: 601 EVNEYLSFLCALGYNKAQISQFSNGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKN 660

Query: 697 VGSPGTYKAEIRKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIW 756
           VGSPGTYKAEIRKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIW
Sbjct: 661 VGSPGTYKAEIRKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIW 720

Query: 757 SDGKHHVRSPIVVKVV 772
           SDGKHHVRSPIVVKVV
Sbjct: 721 SDGKHHVRSPIVVKVV 736

BLAST of CsGy7G002160 vs. ExPASy TrEMBL
Match: A0A5D3BUG6 (Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84663G00160 PE=3 SV=1)

HSP 1 Score: 1475 bits (3819), Expect = 0.0
Identity = 734/773 (94.95%), Postives = 755/773 (97.67%), Query Frame = 0

Query: 1   MKLSTLSSSILLLS-FLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFL 60
           MKLSTLSSSI LLS FLLLSSSLFTPTSAIKRSYVVYMGAHSHGG+KPADVVA+SHH+FL
Sbjct: 1   MKLSTLSSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 60

Query: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRS 120
           QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVA QLAKHP+VVSVFLNRGRKLHTTRS
Sbjct: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 120

Query: 121 WEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGIC 180
           WEFMGLEN+NGVI SESIWKKARFGEDTIIGNLDTGVWAESKSFSDDE GPIPHRWKGIC
Sbjct: 121 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 180

Query: 181 QNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240
           QNQ DPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA
Sbjct: 181 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240

Query: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGG 300
           SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFD AIHDGVDVLSVSLGG
Sbjct: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 300

Query: 301 DPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSL 360
           DPN LFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKF SL
Sbjct: 301 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 360

Query: 361 VVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKI 420
           VVLGN+KQIEGESLSQ+ALPSKKLYPLMNAADVRLANAS HEAQLCKAGTL+PMKAKGKI
Sbjct: 361 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 420

Query: 421 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAY 480
           LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPA HIN+TDGSAVFAY
Sbjct: 421 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 480

Query: 481 INSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 540
           INSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA
Sbjct: 481 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 540

Query: 541 EGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDN 600
           EGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL+TLYPHWSPAAIKSAIMTTAS LDN
Sbjct: 541 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 600

Query: 601 NFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFS 660
           NFEPLLNASY VA+PFNYGAGHVHPNGA DPGLVYDI+VNEYLSFLCALGY+KAQISQFS
Sbjct: 601 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 660

Query: 661 NGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKP 720
           +GPFNCS+PIS TNLNYPSITVPKLSRSITITRRLKNVGSPGTY+A+IRKPAGISVWVKP
Sbjct: 661 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 720

Query: 721 KKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 772
           KKL+FT+LGEELSFKV MKVK  KVAKKNYVYGDLIWSDGKHHVRSPIVVKVV
Sbjct: 721 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of CsGy7G002160 vs. ExPASy TrEMBL
Match: A0A1S3CG03 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500491 PE=3 SV=1)

HSP 1 Score: 1474 bits (3816), Expect = 0.0
Identity = 733/773 (94.83%), Postives = 755/773 (97.67%), Query Frame = 0

Query: 1   MKLSTLSSSILLLS-FLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFL 60
           M+LSTLSSSI LLS FLLLSSSLFTPTSAIKRSYVVYMGAHSHGG+KPADVVA+SHH+FL
Sbjct: 1   MRLSTLSSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 60

Query: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRS 120
           QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVA QLAKHP+VVSVFLNRGRKLHTTRS
Sbjct: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 120

Query: 121 WEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGIC 180
           WEFMGLEN+NGVI SESIWKKARFGEDTIIGNLDTGVWAESKSFSDDE GPIPHRWKGIC
Sbjct: 121 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 180

Query: 181 QNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240
           QNQ DPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA
Sbjct: 181 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240

Query: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGG 300
           SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFD AIHDGVDVLSVSLGG
Sbjct: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 300

Query: 301 DPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSL 360
           DPN LFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKF SL
Sbjct: 301 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 360

Query: 361 VVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKI 420
           VVLGN+KQIEGESLSQ+ALPSKKLYPLMNAADVRLANAS HEAQLCKAGTL+PMKAKGKI
Sbjct: 361 VVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKI 420

Query: 421 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAY 480
           LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPA HIN+TDGSAVFAY
Sbjct: 421 LVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAY 480

Query: 481 INSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 540
           INSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA
Sbjct: 481 INSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEA 540

Query: 541 EGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDN 600
           EGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL+TLYPHWSPAAIKSAIMTTAS LDN
Sbjct: 541 EGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 600

Query: 601 NFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFS 660
           NFEPLLNASY VA+PFNYGAGHVHPNGA DPGLVYDI+VNEYLSFLCALGY+KAQISQFS
Sbjct: 601 NFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFS 660

Query: 661 NGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKP 720
           +GPFNCS+PIS TNLNYPSITVPKLSRSITITRRLKNVGSPGTY+A+IRKPAGISVWVKP
Sbjct: 661 DGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKP 720

Query: 721 KKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 772
           KKL+FT+LGEELSFKV MKVK  KVAKKNYVYGDLIWSDGKHHVRSPIVVKVV
Sbjct: 721 KKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of CsGy7G002160 vs. ExPASy TrEMBL
Match: A0A5A7UYI5 (Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G002010 PE=3 SV=1)

HSP 1 Score: 1404 bits (3635), Expect = 0.0
Identity = 707/775 (91.23%), Postives = 734/775 (94.71%), Query Frame = 0

Query: 1   MKLSTLSSSILLLS-FLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFL 60
           MKLSTLSSSI LLS FLLLSSSLFTPTSAIKRSYVVYMGAHSHGG+KPADVVA+SHH+FL
Sbjct: 1   MKLSTLSSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 60

Query: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRS 120
           QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVA QLAKHP+VVSVFLNRGRKLHTTRS
Sbjct: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 120

Query: 121 WEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGIC 180
           WEFMGLEN+NGVI SESIWKKARFGEDTIIGNLDTGVWAESKSFSDDE GPIPHRWKGIC
Sbjct: 121 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEMGPIPHRWKGIC 180

Query: 181 QNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240
           QNQ DPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA
Sbjct: 181 QNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGA 240

Query: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGG 300
           SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFD AIHDGVDVLSVSLGG
Sbjct: 241 SVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGG 300

Query: 301 DPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSL 360
           DPN LFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKF SL
Sbjct: 301 DPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSL 360

Query: 361 VVLGNRKQIE--GESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKG 420
           VVLGN+KQIE  G  +    L  +     +N   + L + +   +QLCKAGTL+PMKAKG
Sbjct: 361 VVLGNKKQIEETGTIIFCSLLAIEYTAYYLNYQYLTLTSWTPF-SQLCKAGTLDPMKAKG 420

Query: 421 KILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVF 480
           KILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPA HIN+TDGSAVF
Sbjct: 421 KILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVF 480

Query: 481 AYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYT 540
           AYINSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYT
Sbjct: 481 AYINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYT 540

Query: 541 EAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASIL 600
           EAEGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL+TLYPHWSPAAIKSAIMTTAS L
Sbjct: 541 EAEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTL 600

Query: 601 DNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQ 660
           DNNFEPLLNASY VA+PFNYGAGHVHPNGA DPGLVYDI+VNEYLSFLCALGY+KAQISQ
Sbjct: 601 DNNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQ 660

Query: 661 FSNGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWV 720
           FS+GPFNCS+PIS TNLNYPSITVPKLSRSITITRRLKNVGSPGTY+A+IRKPAGISVWV
Sbjct: 661 FSDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWV 720

Query: 721 KPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 772
           KPKKL+FT+LGEELSFKV MKVK  KVAKKNYVYGDLIWSDGKHHVRSPIVVKVV
Sbjct: 721 KPKKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 774

BLAST of CsGy7G002160 vs. ExPASy TrEMBL
Match: A0A6J1HWD2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468122 PE=3 SV=1)

HSP 1 Score: 1351 bits (3496), Expect = 0.0
Identity = 665/759 (87.62%), Postives = 709/759 (93.41%), Query Frame = 0

Query: 16  LLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIF 75
           LLLSS L TPT+AIKRSYVVYMGAHSHGG+KPAD+VA+SH E L+P+L+SG EF +DVIF
Sbjct: 36  LLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPADIVADSHRELLRPYLESGNEFAEDVIF 95

Query: 76  YSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRSWEFMGLENKNGVINSE 135
           YSYTRHINGFAAMLEDEVA +LAKHP+VVSVF NRGR+LHTTRSWEFM LEN +GVI+SE
Sbjct: 96  YSYTRHINGFAAMLEDEVAARLAKHPEVVSVFPNRGRRLHTTRSWEFMELENNHGVISSE 155

Query: 136 SIWKKARFGEDTIIGNLDTGVWAESKSFSD-DEYGPIPHRWKGICQNQKDPSFHCNRKLI 195
           SIWKKARFGEDTIIGNLDTGVW ESKSFSD D+ GPIP RW+GICQNQ DPSFHCNRKLI
Sbjct: 156 SIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQRWRGICQNQNDPSFHCNRKLI 215

Query: 196 GARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSP 255
           GARYFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGASVFGLGKG AKGGSP
Sbjct: 216 GARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGGSP 275

Query: 256 RARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGGDPNALFNDSVAIGSF 315
           RARVAAYKVCWPP AGNECFDADILAAFD AIHDGVDVLSVSLGGDPN LFNDSVAIGSF
Sbjct: 276 RARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSVAIGSF 335

Query: 316 HAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSLVVLGNRKQIEGESLS 375
           HA++HGIVVICSAGNSGP AG+VTN+APWQITVGASTMDRKF S VVLGN+KQIEGESL+
Sbjct: 336 HAVRHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQIEGESLT 395

Query: 376 QDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKILVCLRGDNARVDKGE 435
            + LPS+K YPLM+AADVRLANAS HEAQLCKAGTL+P KAKGKILVCLRGDNARVDKGE
Sbjct: 396 PEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARVDKGE 455

Query: 436 QALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAYINSTKYPEAYITPAT 495
           QA+LAGA GMILANNELSGNEILADPHVLPASHINFTDG AVFAYINSTK+P+AYIT AT
Sbjct: 456 QAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAYITGAT 515

Query: 496 TQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRIPF 555
           TQLG RPAPFMAAFSSVGP+T+TPEILKPD+TAPGLSVIAAYTEAEGPTNQE+D RR+ F
Sbjct: 516 TQLGTRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTEAEGPTNQEYDKRRVAF 575

Query: 556 NSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDNNFEPLLNASYSVASP 615
           NSVSGTSMSCPHVSGIAGLL+TLYPHWSPAAIKSAIMTTAS LDNNF+PLLNASYSVA+P
Sbjct: 576 NSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFKPLLNASYSVATP 635

Query: 616 FNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFSNGPFNCSDPISPTNL 675
           FNYGAGH+HPN A DPGLVYDI + EYLSFLCALGYNKAQ+SQFSNGPFNCS+PIS TNL
Sbjct: 636 FNYGAGHIHPNKATDPGLVYDITITEYLSFLCALGYNKAQLSQFSNGPFNCSEPISLTNL 695

Query: 676 NYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKPKKLSFTRLGEELSFK 735
           NYPSITVP LSRSITITRRLKNVGSPGTYKAEIR+PAGISV VKPKKLSFTRLGEELSFK
Sbjct: 696 NYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRRPAGISVSVKPKKLSFTRLGEELSFK 755

Query: 736 VLMKVKERKVAK-KNYVYGDLIWSDGKHHVRSPIVVKVV 772
           VLMKVKE  V + KNYVYGDLIWSDGKHHVRSPIVV  +
Sbjct: 756 VLMKVKEGNVGREKNYVYGDLIWSDGKHHVRSPIVVMAI 794

BLAST of CsGy7G002160 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 974.2 bits (2517), Expect = 6.3e-284
Identity = 494/776 (63.66%), Postives = 598/776 (77.06%), Query Frame = 0

Query: 1   MKLSTLSSSILLLSFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADV---VANSHHE 60
           MKL T + S LLL  L+  SS     S    SYVVY GAHSH G+   D    V  +H++
Sbjct: 1   MKL-THNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYD 60

Query: 61  FLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTT 120
           FL  F  S E  T D IFYSYT+HINGFAA L+ ++A +++KHP+VVSVF N+  KLHTT
Sbjct: 61  FLGSFTGSRERAT-DAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTT 120

Query: 121 RSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKG 180
           RSW+F+GLE+ N  + S SIW+KARFGEDTII NLDTGVW ESKSF D+  GPIP RWKG
Sbjct: 121 RSWDFLGLEH-NSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKG 180

Query: 181 ICQNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVA 240
           ICQNQKD +FHCNRKLIGARYFNKGYA+ VG LNSSF SPRD +GHGSHTLSTA G+FV 
Sbjct: 181 ICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVP 240

Query: 241 GASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSL 300
           G S+FG G GTAKGGSPRARVAAYKVCWPP  GNEC+DAD+LAAFD AIHDG DV+SVSL
Sbjct: 241 GVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSL 300

Query: 301 GGDPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFL 360
           GG+P + FNDSVAIGSFHA K  IVV+CSAGNSGPA  TV+NVAPWQITVGASTMDR+F 
Sbjct: 301 GGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFA 360

Query: 361 SLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKG 420
           S +VLGN K  +G+SLS  ALP  K YP+M + + +  NAS  +AQLCK G+L+P+K KG
Sbjct: 361 SNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKG 420

Query: 421 KILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVF 480
           KILVCLRG N RV+KG    L G  GM+L N  ++GN++LADPHVLPA+ +   D  AV 
Sbjct: 421 KILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVS 480

Query: 481 AYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYT 540
            YI+ TK P A+ITP+ T LG++PAP MA+FSS GP+ V P+ILKPDITAPG+SVIAAYT
Sbjct: 481 RYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYT 540

Query: 541 EAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASIL 600
            A  PTN++FD RR+ FN++SGTSMSCPH+SGIAGLLKT YP WSPAAI+SAIMTTA+I+
Sbjct: 541 GAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIM 600

Query: 601 DNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQ 660
           D+   P+ NA+   A+PF++GAGHV PN A +PGLVYD+ + +YL+FLC+LGYN +QIS 
Sbjct: 601 DDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISV 660

Query: 661 FSNGPFNCSDP-ISPTNLNYPSITVPKLSRS-ITITRRLKNVGSPGTYKAEIRKPAGISV 720
           FS   F CS P IS  NLNYPSITVP L+ S +T++R +KNVG P  Y  ++  P G+ V
Sbjct: 661 FSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYV 720

Query: 721 WVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKV 772
            VKP  L+FT++GE+ +FKV++ VK +    K YV+G+L+WSD KH VRSPIVVK+
Sbjct: 721 AVKPTSLNFTKVGEQKTFKVIL-VKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of CsGy7G002160 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 915.2 bits (2364), Expect = 3.5e-266
Identity = 457/765 (59.74%), Postives = 576/765 (75.29%), Query Frame = 0

Query: 12  LLSFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPA---DVVANSHHEFLQPFLKSGEE 71
           L S LLL +  F+P  A+K+SY+VY+G+H+H  Q  +   D VA+SH  FL  F+ S E 
Sbjct: 21  LSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN 80

Query: 72  FTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRSWEFMGLENK 131
             K+ IFYSY RHINGFAA+L++  A ++AKHP VVSVF N+GRKLHTT SW FM L  K
Sbjct: 81  -AKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFM-LLAK 140

Query: 132 NGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGICQNQKDPSFH 191
           NGV++  S+W KA +GEDTII NLDTGVW ESKSFSD+ YG +P RWKG C         
Sbjct: 141 NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHK----DVP 200

Query: 192 CNRKLIGARYFNKGYASVVG-PLNSSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKG 251
           CNRKLIGARYFNKGY +  G P N+S+ + RD +GHGSHTLSTA GNFV GA+VFG+G G
Sbjct: 201 CNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNG 260

Query: 252 TAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGGDPNALFND 311
           TA GGSP+ARVAAYKVCWPP  G ECFDADILAA + AI DGVDVLS S+GGD     +D
Sbjct: 261 TASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSD 320

Query: 312 SVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSLVVLGNRKQ 371
            +AIGSFHA+K+G+ V+CSAGNSGP +GTV+NVAPW ITVGAS+MDR+F + V L N + 
Sbjct: 321 GIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQS 380

Query: 372 IEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKILVCLRGDN 431
            +G SLS+  LP +K+Y L++AAD  +AN +V +A LCK G+L+P K KGKILVCLRGDN
Sbjct: 381 FKGTSLSK-PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDN 440

Query: 432 ARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAYINSTKYPE 491
           ARVDKG QA  AGAAGM+L N++ SGNEI++D HVLPAS I++ DG  +F+Y++STK P+
Sbjct: 441 ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPK 500

Query: 492 AYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAEGPTNQEF 551
            YI   T  L  +PAPFMA+FSS GPNT+TP ILKPDITAPG+++IAA+TEA GPT+ + 
Sbjct: 501 GYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDS 560

Query: 552 DNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDNNFEPLLNA 611
           DNRR PFN+ SGTSMSCPH+SG+ GLLKTL+PHWSPAAI+SAIMTT+   +N  +P+++ 
Sbjct: 561 DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDE 620

Query: 612 SYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFSNGP-FNCS 671
           S+  A+PF+YG+GHV PN AA PGLVYD+   +YL FLCA+GYN   +  F+  P + C 
Sbjct: 621 SFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCR 680

Query: 672 DPISPTNLNYPSITVPKLSRSITITRRLKNVGSPGTYKAEIRKPAGISVWVKPKKLSFTR 731
              +  + NYPSITVP L+ SIT+TR+LKNVG P TY A  R+P G+ V V+PK+L+F +
Sbjct: 681 QGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNK 740

Query: 732 LGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRSPIVVKV 772
            GE   F+  M ++   V    YV+G+L W+D  H+VRSPIVV++
Sbjct: 741 TGEVKIFQ--MTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of CsGy7G002160 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 597.8 bits (1540), Expect = 1.2e-170
Identity = 349/792 (44.07%), Postives = 467/792 (58.96%), Query Frame = 0

Query: 16  LLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIF 75
           LL    L    +  K+ Y+VY G H   G K    +   HH +LQ  +K  EE  +  + 
Sbjct: 10  LLFLIPLLASCAEEKQVYIVYFGEHK--GDKAFHEIEEHHHSYLQS-VKESEEDARASLL 69

Query: 76  YSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRK--LHTTRSWEFMGLE------- 135
           YSY   INGFAA L  + A +L K  +VVSVF +  RK   HTTRSWEF+GLE       
Sbjct: 70  YSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSD 129

Query: 136 ---NKNGVIN----SESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGIC 195
               KN   +      +  KKA+ G+  I+G LD+GVW ESKSF+D   GP+P  WKGIC
Sbjct: 130 VPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGIC 189

Query: 196 Q-NQKDPSFHCNRKLIGARYFNKGYASVVGPLNSS----FHSPRDKEGHGSHTLSTAGGN 255
           Q      S HCNRK+IGARY+ KGY    G  N++    F SPRD +GHGSHT STA G 
Sbjct: 190 QTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGR 249

Query: 256 FVAGASVF-GLGKGTAKGGSPRARVAAYKVCW-PPKA----GNECFDADILAAFDFAIHD 315
            V GAS   G  KG+A GG+P AR+A YK CW  P A    GN C + D+LAA D AI D
Sbjct: 250 RVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIAD 309

Query: 316 GVDVLSVSLG-GDPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITV 375
           GV V+S+S+G  +P     D +A+G+ HA+K  IVV  SAGNSGP  GT++N+APW ITV
Sbjct: 310 GVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITV 369

Query: 376 GASTMDRKFLSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLANASVHEAQLCKA 435
           GAST+DR F+  +VLGN   I+ +S++  A    K  PL+ A++V +   +++E   C  
Sbjct: 370 GASTLDRAFVGGLVLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNETSQCLP 429

Query: 436 GTLNPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASH 495
            +L P    GK+++CLRG  +R+ KG +   AG AGMIL N   +GNE+ +D H +P + 
Sbjct: 430 NSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAG 489

Query: 496 INFTDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITA 555
           +  T    +  YI + K P+A+I P  T    + AP M  FSS GPN V P ILKPDITA
Sbjct: 490 VTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITA 549

Query: 556 PGLSVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIK 615
           PGL ++AA++ A+ P+    D R   +N  SGTSMSCPHV+G   LLK ++P WS AAI+
Sbjct: 550 PGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIR 609

Query: 616 SAIMTTASILDNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCA 675
           SA+MTTA + ++  +P+ + +   A+PF  G+GH  P  AADPGLVYD     YL + C+
Sbjct: 610 SALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYLLYGCS 669

Query: 676 LGYNKAQISQFSNGPFNCSDPISP-TNLNYPSITVPKLSRSITITRRLKNVG---SPGTY 735
           +           +  F C   I P  N NYPSI VP L +++T+ R + NVG   S  TY
Sbjct: 670 VNITNI------DPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTY 729

Query: 736 KAEIRKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKV----AKKNYVYGDLIWSDG 772
              ++ P+GISV   P  LSF R+G++  FK+++K  + +V     K  Y +G   W+D 
Sbjct: 730 LFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDK 789

BLAST of CsGy7G002160 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 596.3 bits (1536), Expect = 3.6e-170
Identity = 351/784 (44.77%), Postives = 486/784 (61.99%), Query Frame = 0

Query: 1   MKLSTLSSS----ILLLSFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPA--DVVANS 60
           M  S LSS+    +L L F  +SSS     S+ + +Y+V+M       Q P+  D+ +N 
Sbjct: 1   MSSSFLSSTAFFLLLCLGFCHVSSS-----SSDQGTYIVHMAK----SQMPSSFDLHSNW 60

Query: 61  HHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKL 120
           +   L+    S E      + Y+Y   I+GF+  L  E A  L   P V+SV      +L
Sbjct: 61  YDSSLRSISDSAE------LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYEL 120

Query: 121 HTTRSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHR 180
           HTTR+  F+GL+      ++  ++ +A    D ++G LDTGVW ESKS+SD+ +GPIP  
Sbjct: 121 HTTRTPLFLGLDE-----HTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSS 180

Query: 181 WKGICQ-NQKDPSFHCNRKLIGARYFNKGYASVVGPLNSS--FHSPRDKEGHGSHTLSTA 240
           WKG C+      +  CNRKLIGAR+F +GY S +GP++ S    SPRD +GHG+HT STA
Sbjct: 181 WKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTA 240

Query: 241 GGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVD 300
            G+ V GAS+ G   GTA+G +PRARVA YKVCW       CF +DILAA D AI D V+
Sbjct: 241 AGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVN 300

Query: 301 VLSVSLGGDPNALFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGAST 360
           VLS+SLGG  +  + D VAIG+F A++ GI+V CSAGN+GP++ +++NVAPW  TVGA T
Sbjct: 301 VLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGT 360

Query: 361 MDRKFLSLVVLGNRKQIEGESLSQ-DALPSKKLYPLMNAADVRLANASVHEAQLCKAGTL 420
           +DR F +L +LGN K   G SL + +ALP  KL P + A +   +NA+     LC  GTL
Sbjct: 361 LDRDFPALAILGNGKNFTGVSLFKGEALPD-KLLPFIYAGNA--SNAT--NGNLCMTGTL 420

Query: 421 NPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINF 480
            P K KGKI++C RG NARV KG+    AG  GMILAN   +G E++AD H+LPA+ +  
Sbjct: 421 IPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGE 480

Query: 481 TDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGL 540
             G  +  Y+ +   P A I+   T +G++P+P +AAFSS GPN++TP ILKPD+ APG+
Sbjct: 481 KAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGV 540

Query: 541 SVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAI 600
           +++AA+T A GPT    D+RR+ FN +SGTSMSCPHVSG+A LLK+++P WSPAAI+SA+
Sbjct: 541 NILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSAL 600

Query: 601 MTTASILDNNFEPLLN-ASYSVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALG 660
           MTTA     + +PLL+ A+   ++PF++GAGHV P  A +PGL+YD+   +YL FLCAL 
Sbjct: 601 MTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALN 660

Query: 661 YNKAQISQFSNGPFNC--SDPISPTNLNYPSITVPKLS-RSITITRRLKNVGSPGTYKAE 720
           Y   QI   S   + C  S   S  +LNYPS  V      +   TR + +VG  GTY  +
Sbjct: 661 YTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVK 720

Query: 721 I-RKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIWSDGKHHVRS 770
           +  +  G+ + V+P  L+F    E+ S+ V   V   K +  N  +G + WSDGKH V S
Sbjct: 721 VTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGS 754

BLAST of CsGy7G002160 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 590.1 bits (1520), Expect = 2.6e-168
Identity = 344/776 (44.33%), Postives = 473/776 (60.95%), Query Frame = 0

Query: 16  LLLSSSLFTPTSAIKRSYVVYMGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIF 75
           L LSS   + +S+   +Y+V++   +    KP+  +  +H  +    L S       +I 
Sbjct: 11  LTLSSPSSSASSSNSLTYIVHVDHEA----KPS--IFPTHFHWYTSSLASLTSSPPSII- 70

Query: 76  YSYTRHINGFAAMLEDEVAVQLAKHPKVVSVFLNRGRKLHTTRSWEFMGLENKNGVINSE 135
           ++Y    +GF+A L  + A QL  HP V+SV   + R LHTTRS EF+GL +     +  
Sbjct: 71  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS----TDKA 130

Query: 136 SIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGICQNQKD-PSFHCNRKLI 195
            + +++ FG D +IG +DTGVW E  SF D   GP+P +WKG C   +D P   CNRKL+
Sbjct: 131 GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLV 190

Query: 196 GARYFNKGYASVVGPLN--SSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGG 255
           GAR+F  GY +  G +N  + F SPRD +GHG+HT S + G +V  AS  G   G A G 
Sbjct: 191 GARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGM 250

Query: 256 SPRARVAAYKVCWPPKAGNECFDADILAAFDFAIHDGVDVLSVSLGGDPNALFNDSVAIG 315
           +P+AR+AAYKVCW     + C+D+DILAAFD A+ DGVDV+S+S+GG     + D++AIG
Sbjct: 251 APKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIG 310

Query: 316 SFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFLSLVVLGNRKQIEGES 375
           +F AI  GI V  SAGN GP A TVTNVAPW  TVGA T+DR F + V LGN K I G S
Sbjct: 311 AFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVS 370

Query: 376 L-SQDALPSKKLYPLMNAADVRLANASVHEAQLCKAGTLNPMKAKGKILVCLRGDNARVD 435
           +     L   ++YPL+      L     + + LC  G+L+P   KGKI++C RG N+R  
Sbjct: 371 VYGGPGLDPGRMYPLVYGGS--LLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRAT 430

Query: 436 KGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSAVFAYIN------STKY 495
           KGE     G  GMI+AN    G  ++AD HVLPA+ +  + G  +  YI+      S+K+
Sbjct: 431 KGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKH 490

Query: 496 PEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAEGPTNQ 555
           P A I    T+LGIRPAP +A+FS+ GPN  TPEILKPD+ APGL+++AA+ +  GP+  
Sbjct: 491 PTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGV 550

Query: 556 EFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASILDNNFEPLL 615
             DNRR  FN +SGTSM+CPHVSG+A LLK  +P WSPAAI+SA++TTA  +DN+ EP++
Sbjct: 551 TSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMM 610

Query: 616 NASY-SVASPFNYGAGHVHPNGAADPGLVYDIEVNEYLSFLCALGYNKAQISQFSNGPFN 675
           + S  + +S  +YG+GHVHP  A DPGLVYDI   +Y++FLC   Y +  I   +    +
Sbjct: 611 DESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD 670

Query: 676 CSDPISP---TNLNYPSITV-----PKLSRSITITRRLKNVG-SPGTYKAEIRKPAGISV 735
           C          NLNYPS +V      +   S    R + NVG S   Y+ +IR P G +V
Sbjct: 671 CDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTV 730

Query: 736 WVKPKKLSFTRLGEELSFKVLMKVKERKVA--KKNYVYGDLIWSDGKHHVRSPIVV 770
            V+P+KLSF R+G++LSF V +K  E K++    N   G ++WSDGK +V SP+VV
Sbjct: 731 TVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVV 769