CsGy7G001875 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy7G001875
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	RING/U-box superfamily protein
Location	Gy14Chr7: 1604500 .. 1604694 (+)
RNA-Seq Expression	CsGy7G001875
Synteny	CsGy7G001875

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGAATTGGGAGATTGCAGCATCTGTTTGGATGAATTGAGTTGTGAAAAGAGGGAGGTGATGAGGATTCCTTGTGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTGAGAATCACAATTCTTGTCCTTTGTGTAGAAAGCCATTGCACCACGACGACGACGACGAGGAGGAGTACTCATGGTGA

mRNA sequence

ATGGAGGAATTGGGAGATTGCAGCATCTGTTTGGATGAATTGAGTTGTGAAAAGAGGGAGGTGATGAGGATTCCTTGTGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTGAGAATCACAATTCTTGTCCTTTGTGTAGAAAGCCATTGCACCACGACGACGACGACGAGGAGGAGTACTCATGGTGA

Coding sequence (CDS)

ATGGAGGAATTGGGAGATTGCAGCATCTGTTTGGATGAATTGAGTTGTGAAAAGAGGGAGGTGATGAGGATTCCTTGTGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTGAGAATCACAATTCTTGTCCTTTGTGTAGAAAGCCATTGCACCACGACGACGACGACGAGGAGGAGTACTCATGGTGA

Protein sequence

MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEEEYSW*

Homology

[Close]

BLAST of CsGy7G001875 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 8.1e-12
Identity = 28/61 (45.90%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 3   ELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEEEY 62
           E+  C++C+DE   +  +V ++PC HV+H+ C+  WLE HNSCP+CR  L  DD D E  
Sbjct: 212 EMNQCAVCMDEFE-DGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENR 271

Query: 63  S 64
           S
Sbjct: 272 S 271

[Close]

BLAST of CsGy7G001875 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.8e-11
Identity = 27/58 (46.55%), Postives = 37/58 (63.79%), Query Frame = 0


Query: 3   ELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE 61
           E   C++C+D+   E  E  ++PC H+YH+ C+  WLE HNSCP+CR  L  DD D E
Sbjct: 220 EFNQCAVCMDDFE-EGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYE 276

[Close]

BLAST of CsGy7G001875 vs. ExPASy Swiss-Prot
Match: Q9VHI7 (E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.0e-10
Identity = 25/55 (45.45%), Postives = 38/55 (69.09%), Query Frame = 0


Query: 7   CSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEEE 62
           CSIC D+   ++  V ++PC H+YHE+CI  WL  H++CP+CRK L  D +D ++
Sbjct: 253 CSICWDDFKIDE-TVRKLPCSHLYHENCIVPWLNLHSTCPICRKSLADDGNDADD 306

[Close]

BLAST of CsGy7G001875 vs. ExPASy Swiss-Prot
Match: Q9Y7K6 (Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC2A9.04c PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.4e-10
Identity = 22/50 (44.00%), Postives = 39/50 (78.00%), Query Frame = 0


Query: 7   CSICLDELS-CEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHD 56
           C IC D+++  ++++  ++PCGH++ ++C+ KWLENH +CPLCRK + H+
Sbjct: 107 CPICYDDMNENDEKQATKMPCGHIFGKNCLQKWLENHCTCPLCRKEVPHE 156

[Close]

BLAST of CsGy7G001875 vs. ExPASy Swiss-Prot
Match: Q6AXU4 (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 5.8e-10
Identity = 27/56 (48.21%), Postives = 35/56 (62.50%), Query Frame = 0


Query: 7   CSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEEEY 63
           C +CL E   E+  V+ +PC H++H +CI  WL   NSCPLCR  L  DDD  EE+
Sbjct: 88  CPVCLLEFE-EEETVIEMPCHHLFHSNCILPWLSKTNSCPLCRHELPTDDDSYEEH 142

[Close]

BLAST of CsGy7G001875 vs. NCBI nr
Match: XP_011659694.1 (E3 ubiquitin-protein ligase RDUF1 [Cucumis sativus] >KGN43356.1 hypothetical protein Csa_020163 [Cucumis sativus])

HSP 1 Score: 148 bits (374), Expect = 4.51e-43
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1   MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE 60
           MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE
Sbjct: 167 MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE 226

Query: 61  EYSW 64
           EYSW
Sbjct: 227 EYSW 230

[Close]

BLAST of CsGy7G001875 vs. NCBI nr
Match: XP_004144954.1 (uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical protein Csa_020356 [Cucumis sativus])

HSP 1 Score: 145 bits (367), Expect = 5.17e-42
Identity = 62/64 (96.88%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1   MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE 60
           MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDD+D+E
Sbjct: 167 MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDEDDE 226

Query: 61  EYSW 64
           EYSW
Sbjct: 227 EYSW 230

[Close]

BLAST of CsGy7G001875 vs. NCBI nr
Match: XP_016899530.1 (PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29842.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 118 bits (295), Expect = 3.77e-31
Identity = 51/63 (80.95%), Postives = 58/63 (92.06%), Query Frame = 0


Query: 1   MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE 60
           +E+LGDCSICLDELSCEKREV+RIPCGHVYHESCIFKWL+NHNSCPLCRKPL   +D+E+
Sbjct: 168 VEDLGDCSICLDELSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLKEDEED 227

Query: 61  EYS 63
             S
Sbjct: 228 SSS 230

[Close]

BLAST of CsGy7G001875 vs. NCBI nr
Match: XP_038883574.1 (E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida])

HSP 1 Score: 108 bits (271), Expect = 1.42e-27
Identity = 46/56 (82.14%), Postives = 51/56 (91.07%), Query Frame = 0


Query: 2   EELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDD 57
           EELGDC ICL+ELSCEKREV+RIPCGHVYHESC+FKWL  +NSCPLCRKPL  +DD
Sbjct: 170 EELGDCCICLEELSCEKREVIRIPCGHVYHESCVFKWLRKNNSCPLCRKPLPLNDD 225

[Close]

BLAST of CsGy7G001875 vs. NCBI nr
Match: XP_016901482.1 (PREDICTED: uncharacterized protein LOC107991269 [Cucumis melo] >KAA0044413.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29540.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 97.8 bits (242), Expect = 2.87e-23
Identity = 40/48 (83.33%), Postives = 44/48 (91.67%), Query Frame = 0


Query: 2   EELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCR 49
           EE GDCS+CLDEL C KREV+RIPCGHVYHESCIF+WL N+NSCPLCR
Sbjct: 165 EEEGDCSVCLDELDCGKREVIRIPCGHVYHESCIFEWLNNNNSCPLCR 212

[Close]

BLAST of CsGy7G001875 vs. ExPASy TrEMBL
Match: A0A0A0K696 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 148 bits (374), Expect = 2.18e-43
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1   MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE 60
           MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE
Sbjct: 167 MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE 226

Query: 61  EYSW 64
           EYSW
Sbjct: 227 EYSW 230

[Close]

BLAST of CsGy7G001875 vs. ExPASy TrEMBL
Match: A0A0A0K2W2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 145 bits (367), Expect = 2.50e-42
Identity = 62/64 (96.88%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1   MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE 60
           MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDD+D+E
Sbjct: 167 MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDEDDE 226

Query: 61  EYSW 64
           EYSW
Sbjct: 227 EYSW 230

[Close]

BLAST of CsGy7G001875 vs. ExPASy TrEMBL
Match: A0A5D3E1S5 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold208G00800 PE=4 SV=1)

HSP 1 Score: 118 bits (295), Expect = 1.83e-31
Identity = 51/63 (80.95%), Postives = 58/63 (92.06%), Query Frame = 0


Query: 1   MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE 60
           +E+LGDCSICLDELSCEKREV+RIPCGHVYHESCIFKWL+NHNSCPLCRKPL   +D+E+
Sbjct: 168 VEDLGDCSICLDELSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLKEDEED 227

Query: 61  EYS 63
             S
Sbjct: 228 SSS 230

[Close]

BLAST of CsGy7G001875 vs. ExPASy TrEMBL
Match: A0A1S4DUZ4 (E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 PE=4 SV=1)

HSP 1 Score: 118 bits (295), Expect = 1.83e-31
Identity = 51/63 (80.95%), Postives = 58/63 (92.06%), Query Frame = 0


Query: 1   MEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEE 60
           +E+LGDCSICLDELSCEKREV+RIPCGHVYHESCIFKWL+NHNSCPLCRKPL   +D+E+
Sbjct: 168 VEDLGDCSICLDELSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLKEDEED 227

Query: 61  EYS 63
             S
Sbjct: 228 SSS 230

[Close]

BLAST of CsGy7G001875 vs. ExPASy TrEMBL
Match: A0A0A0KYC0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003650 PE=4 SV=1)

HSP 1 Score: 102 bits (254), Expect = 1.36e-25
Identity = 44/54 (81.48%), Postives = 47/54 (87.04%), Query Frame = 0


Query: 2   EELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK--PLH 53
           EE GDCS+CLDEL CEKREV+RIPCGHVYHESCIFKWL + NSCPLCR   PLH
Sbjct: 145 EEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLH 198

[Close]

BLAST of CsGy7G001875 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 5.7e-13
Identity = 28/61 (45.90%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 3   ELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEEEY 62
           E+  C++C+DE   +  +V ++PC HV+H+ C+  WLE HNSCP+CR  L  DD D E  
Sbjct: 212 EMNQCAVCMDEFE-DGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENR 271

Query: 63  S 64
           S
Sbjct: 272 S 271

[Close]

BLAST of CsGy7G001875 vs. TAIR 10
Match: AT5G37200.1 (RING/U-box superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 1.3e-12
Identity = 29/62 (46.77%), Postives = 44/62 (70.97%), Query Frame = 0


Query: 2   EELGDCSICLDELSCEKREVMRIP-CGHVYHESCIFKWLENHNSCPLCRK-PLHHDDDDE 61
           EE   CSIC+++ S    +++R+P C H++H+ C+F+WL   NSCPLCRK P   +D+DE
Sbjct: 154 EEDETCSICIEKFSESHEDIIRVPDCLHLFHQGCLFEWLGLQNSCPLCRKVPYEEEDEDE 213

[Close]

BLAST of CsGy7G001875 vs. TAIR 10
Match: AT1G57730.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 1.4e-11
Identity = 26/57 (45.61%), Postives = 38/57 (66.67%), Query Frame = 0


Query: 7   CSICLDELSCEKREVMRIP-CGHVYHESCIFKWLENHNSCPLCRKPLHHDDDDEEEY 63
           C+ICL+++S +  +   +P C HV+H  CI+KWL + N CPLCR  L  +DDD+  Y
Sbjct: 116 CAICLEDMSQDVHDYQEMPNCPHVFHNDCIYKWLGHSNLCPLCRTVLEDEDDDDYPY 172

[Close]

BLAST of CsGy7G001875 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 9.1e-11
Identity = 24/46 (52.17%), Postives = 29/46 (63.04%), Query Frame = 0


Query: 7   CSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPL 53
           C +C DE    K E  ++PC H+YH  CI  WL  HNSCP+CRK L
Sbjct: 186 CPVCKDEFEL-KSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKEL 230

[Close]

BLAST of CsGy7G001875 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 9.1e-11
Identity = 24/46 (52.17%), Postives = 29/46 (63.04%), Query Frame = 0


Query: 7   CSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPL 53
           C +C DE    K E  ++PC H+YH  CI  WL  HNSCP+CRK L
Sbjct: 186 CPVCKDEFEL-KSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKEL 230

The following BLAST results are available for this feature:

BLAST of CsGy7G001875 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	8.1e-12	45.90	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	1.8e-11	46.55	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q9VHI7	2.0e-10	45.45	E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1...	[more]
Q9Y7K6	4.4e-10	44.00	Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strai...	[more]
Q6AXU4	5.8e-10	48.21	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 ...	[more]

BLAST of CsGy7G001875 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659694.1	4.51e-43	100.00	E3 ubiquitin-protein ligase RDUF1 [Cucumis sativus] >KGN43356.1 hypothetical pro...	[more]
XP_004144954.1	5.17e-42	96.88	uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical ...	[more]
XP_016899530.1	3.77e-31	80.95	PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 ...	[more]
XP_038883574.1	1.42e-27	82.14	E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida]	[more]
XP_016901482.1	2.87e-23	83.33	PREDICTED: uncharacterized protein LOC107991269 [Cucumis melo] >KAA0044413.1 E3 ...	[more]

BLAST of CsGy7G001875 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K696	2.18e-43	100.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 P...	[more]
A0A0A0K2W2	2.50e-42	96.88	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G025210 P...	[more]
A0A5D3E1S5	1.83e-31	80.95	E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S4DUZ4	1.83e-31	80.95	E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 ...	[more]
A0A0A0KYC0	1.36e-25	81.48	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003650 P...	[more]

BLAST of CsGy7G001875 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	5.7e-13	45.90	RING/U-box superfamily protein	[more]
AT5G37200.1	1.3e-12	46.77	RING/U-box superfamily protein	[more]
AT1G57730.1	1.4e-11	45.61	RING/U-box superfamily protein	[more]
AT3G56580.2	9.1e-11	52.17	RING/U-box superfamily protein	[more]
AT3G56580.1	9.1e-11	52.17	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 7..48 e-value: 4.4E-8 score: 42.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 6..49 e-value: 1.3E-14 score: 54.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 7..49 score: 13.477729
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..63 e-value: 9.1E-20 score: 71.6
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 4..50
None	No IPR available	PANTHER	PTHR22765:SF329	SUBFAMILY NOT NAMED	coord: 4..50
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 6..55

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy7G001875.1	CsGy7G001875.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031507	heterochromatin assembly
biological_process	GO:0070734	histone H3-K27 methylation
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0048587	regulation of short-day photoperiodism, flowering
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005677	chromatin silencing complex
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0031519	PcG protein complex
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0061630	ubiquitin protein ligase activity