CsGy7G000670 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy7G000670
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
LocationGy14Chr7: 747258 .. 749209 (+)
RNA-Seq ExpressionCsGy7G000670
SyntenyCsGy7G000670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGACTCTTCACCATTTAGTTCTGTAGCCTTCTCATTGCTTCTGATTCTTGTGGCCTTCTGTTCGACTCCAACACATTCGGTAACGGTTCGAGGATTGTCAATTAGAGAAGCCACAGTGCACGACCTCCAGCTAGCTTTCAAGCAAAACCAACTCACTTCGAGGGAACTTGTCAACTTCTACATCGGAGAAATTCGCAGACTCAATCCAGTTGTCCACGGGGTCATAGAAATCAACCCAGATGCATTGCTGCAAGCTTACAAGGCTGACAGAGAACGAGAGGCTAATAAGCCTGGATCGCTTTGTGGGTTGCATGGAATTCCAGTTCTACTCAAGGATAACATTGGGACTAAGGATAAGTTGAATACTACTGCTGGATCATTCGCATTGCTTGGGTCTATAGTGCCTCGTGATGCAGGTGTAGTGAAGAGACTAAGGAAGGCTGGAGCTATAATACTAGGAAAGGCCAGCTTAAGTGAATGGGCTGATTTTAGGTCCCTCGCTGCTCCAGCTGGTTTGAGCGCTAGAGGTGGTCAAGGAAAGGTGTGTTACTTCGAATTGGCTCCAAAGACAATTTATCCACACATTCAATGTTTTTCATGTACAACTAAAAACCATTTAATTTCTACAATTCTTTTCAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACCGAGACCGATGGTTCTATCCTCTGTCCGGCTAGTTTCAACTCAGTTGTTGGCATCAAACCTACGGTCGGCCTCACTAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGTGAGCAATCTTGCACCCATATCAGCAGAACATTCTACAAAGATTGTAAGAACTAAAACTTTGTAACCTTCACATGTGAAAACAGGCCAATTGGCAGGACAGTGACAGACGCTGTGATAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGCAGCCACTAGAACAGCCTCTAAGTACATTCCATATGGTGGCTACAAACAATTCCTCAATCCAAATGGGTTGAAAGGGAAGAGGTTAGGAATTGTGAGAAACCCCTTTTTCAGCTTCTTCAATGATTCAACCATCACACAAGCTTTTGAGGATCATTTCAACACTCTAAAGTAAGAAAACCAACATCCCTACCAATCCATATAATTCCCCCAAAATCTTTCTATCATATTCATTTAAACAAACAATTCAACGTGAACATAACCAATCTACACAAAGAATCTAACTAACATTATTGAAATTGAAGGCAAGGTGGTGCTATTTTGATAGACAATCTAGAGATAGCAGATATAGATATCATCTTAAACGTAACAGCGAGTGGAGAAGCAGCAGCATTGCTTGCTGAATTCAAACAATCGCTAAATGGGTACCTGAAAGAGCTCGTGGTTTCCCCTGTTCGAAGTTTAGCCGACATAATCGCCTTCAATAACGCAAATGCAGATCAGGTAACACAATATTCAATCCATTTTTCATCGCATAGTGATCGTATTCATTCGTGATTGATTCAAATCATGGTTGTGCAGGAACTTCTCAACGTTTTTGGCCAGGAGATCTTTTTGGCGGCCGAAGCCACAAATGGGATCGGCGACGTGCAGAAGGCGGCTGTGTTGAACTTGGGGAAGCTAACAGAAGATGGGTTTGAGAAATTAGTGAAGGAGAATCAATTGGATGCGGTGGTGACTCCAGGTACCGGCATAGCTACAGTGCTCGCAATTGGGGGTTTTCCGGGGATCAATGTTCCGGCTGGATACGACGGCGGAGGAGTTCCGTTTGGGATTAACTTTGGAGGGTTGAAGGGATCAGAGACGAAGCTGATTGAAGTTGCTTATGGGTTTGAGCAAGCCACTCTGATAAGAAAACCTCCTTCCTTCAAACCTTGA

mRNA sequence

ATGGCCGACTCTTCACCATTTAGTTCTGTAGCCTTCTCATTGCTTCTGATTCTTGTGGCCTTCTGTTCGACTCCAACACATTCGGTAACGGTTCGAGGATTGTCAATTAGAGAAGCCACAGTGCACGACCTCCAGCTAGCTTTCAAGCAAAACCAACTCACTTCGAGGGAACTTGTCAACTTCTACATCGGAGAAATTCGCAGACTCAATCCAGTTGTCCACGGGGTCATAGAAATCAACCCAGATGCATTGCTGCAAGCTTACAAGGCTGACAGAGAACGAGAGGCTAATAAGCCTGGATCGCTTTGTGGGTTGCATGGAATTCCAGTTCTACTCAAGGATAACATTGGGACTAAGGATAAGTTGAATACTACTGCTGGATCATTCGCATTGCTTGGGTCTATAGTGCCTCGTGATGCAGGTGTAGTGAAGAGACTAAGGAAGGCTGGAGCTATAATACTAGGAAAGGCCAGCTTAAGTGAATGGGCTGATTTTAGGTCCCTCGCTGCTCCAGCTGGTTTGAGCGCTAGAGGTGGTCAAGGAAAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACCGAGACCGATGGTTCTATCCTCTGTCCGGCTAGTTTCAACTCAGTTGTTGGCATCAAACCTACGGTCGGCCTCACTAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGCCAATTGGCAGGACAGTGACAGACGCTGTGATAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGCAGCCACTAGAACAGCCTCTAAGTACATTCCATATGGTGGCTACAAACAATTCCTCAATCCAAATGGGTTGAAAGGGAAGAGGTTAGGAATTGTGAGAAACCCCTTTTTCAGCTTCTTCAATGATTCAACCATCACACAAGCTTTTGAGGATCATTTCAACACTCTAAAGCAAGGTGGTGCTATTTTGATAGACAATCTAGAGATAGCAGATATAGATATCATCTTAAACGTAACAGCGAGTGGAGAAGCAGCAGCATTGCTTGCTGAATTCAAACAATCGCTAAATGGGTACCTGAAAGAGCTCGTGGTTTCCCCTGTTCGAAGTTTAGCCGACATAATCGCCTTCAATAACGCAAATGCAGATCAGGAACTTCTCAACGTTTTTGGCCAGGAGATCTTTTTGGCGGCCGAAGCCACAAATGGGATCGGCGACGTGCAGAAGGCGGCTGTGTTGAACTTGGGGAAGCTAACAGAAGATGGGTTTGAGAAATTAGTGAAGGAGAATCAATTGGATGCGGTGGTGACTCCAGGTACCGGCATAGCTACAGTGCTCGCAATTGGGGGTTTTCCGGGGATCAATGTTCCGGCTGGATACGACGGCGGAGGAGTTCCGTTTGGGATTAACTTTGGAGGGTTGAAGGGATCAGAGACGAAGCTGATTGAAGTTGCTTATGGGTTTGAGCAAGCCACTCTGATAAGAAAACCTCCTTCCTTCAAACCTTGA

Coding sequence (CDS)

ATGGCCGACTCTTCACCATTTAGTTCTGTAGCCTTCTCATTGCTTCTGATTCTTGTGGCCTTCTGTTCGACTCCAACACATTCGGTAACGGTTCGAGGATTGTCAATTAGAGAAGCCACAGTGCACGACCTCCAGCTAGCTTTCAAGCAAAACCAACTCACTTCGAGGGAACTTGTCAACTTCTACATCGGAGAAATTCGCAGACTCAATCCAGTTGTCCACGGGGTCATAGAAATCAACCCAGATGCATTGCTGCAAGCTTACAAGGCTGACAGAGAACGAGAGGCTAATAAGCCTGGATCGCTTTGTGGGTTGCATGGAATTCCAGTTCTACTCAAGGATAACATTGGGACTAAGGATAAGTTGAATACTACTGCTGGATCATTCGCATTGCTTGGGTCTATAGTGCCTCGTGATGCAGGTGTAGTGAAGAGACTAAGGAAGGCTGGAGCTATAATACTAGGAAAGGCCAGCTTAAGTGAATGGGCTGATTTTAGGTCCCTCGCTGCTCCAGCTGGTTTGAGCGCTAGAGGTGGTCAAGGAAAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACCGAGACCGATGGTTCTATCCTCTGTCCGGCTAGTTTCAACTCAGTTGTTGGCATCAAACCTACGGTCGGCCTCACTAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGCCAATTGGCAGGACAGTGACAGACGCTGTGATAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGCAGCCACTAGAACAGCCTCTAAGTACATTCCATATGGTGGCTACAAACAATTCCTCAATCCAAATGGGTTGAAAGGGAAGAGGTTAGGAATTGTGAGAAACCCCTTTTTCAGCTTCTTCAATGATTCAACCATCACACAAGCTTTTGAGGATCATTTCAACACTCTAAAGCAAGGTGGTGCTATTTTGATAGACAATCTAGAGATAGCAGATATAGATATCATCTTAAACGTAACAGCGAGTGGAGAAGCAGCAGCATTGCTTGCTGAATTCAAACAATCGCTAAATGGGTACCTGAAAGAGCTCGTGGTTTCCCCTGTTCGAAGTTTAGCCGACATAATCGCCTTCAATAACGCAAATGCAGATCAGGAACTTCTCAACGTTTTTGGCCAGGAGATCTTTTTGGCGGCCGAAGCCACAAATGGGATCGGCGACGTGCAGAAGGCGGCTGTGTTGAACTTGGGGAAGCTAACAGAAGATGGGTTTGAGAAATTAGTGAAGGAGAATCAATTGGATGCGGTGGTGACTCCAGGTACCGGCATAGCTACAGTGCTCGCAATTGGGGGTTTTCCGGGGATCAATGTTCCGGCTGGATACGACGGCGGAGGAGTTCCGTTTGGGATTAACTTTGGAGGGTTGAAGGGATCAGAGACGAAGCTGATTGAAGTTGCTTATGGGTTTGAGCAAGCCACTCTGATAAGAAAACCTCCTTCCTTCAAACCTTGA

Protein sequence

MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGEAAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP*
Homology
BLAST of CsGy7G000670 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 6.8e-170
Identity = 319/512 (62.30%), Postives = 396/512 (77.34%), Query Frame = 0

Query: 12  FSLLLILVAFCSTPTHSVTVRG-------LSIREATVHDLQLAFKQNQLTSRELVNFYIG 71
           FS LLIL+        SV            SI+EAT+ D+++AF + +LTS++LV  Y+ 
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 72  EIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKDKLNT 131
            I +LNP++H VIE NPDAL+QA  ADRER+      L  LHG+PVLLKD+I TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 132 TAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNP 191
           TAGSFALLGS+V RDAGVVKRLR++GA+ILGKASLSEWA FRS + P G SARG QGKNP
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNP 188

Query: 192 YVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVI 251
           YVLSA+P GSSSG +ISV AN+ AVS+GTETDGSIL PAS NSVVGIKP+VGLTSRAGV+
Sbjct: 189 YVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVV 248

Query: 252 PVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGK 311
           P+S RQD+IGPI RTV+DAV +LD IVG+D  D AT+TAS++IP GGYKQFL  +GLKGK
Sbjct: 249 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 308

Query: 312 RLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGEAAAL 371
           RLGIV             +   + H  TL++ GAI+I+NL I +I++I+  T SGE  AL
Sbjct: 309 RLGIVMKH----------SSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIAL 368

Query: 372 LAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQ 431
           LAEFK SLN YLKELV SPVRSLAD+IA+N   A+QE +  +GQE+FL AEAT+G+G+ +
Sbjct: 369 LAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKE 428

Query: 432 KAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGGVPFG 491
           K A+  + +L+ +G EKL++EN+LDA+VT G+ +++VLAIGG+PGINVPAGYD GGVP+G
Sbjct: 429 KTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYG 488

Query: 492 INFGGLKGSETKLIEVAYGFEQATLIRKPPSF 517
           I+FGGL+ SE KLIE+A+ FEQATLIRKPP F
Sbjct: 489 ISFGGLRFSEPKLIEIAFAFEQATLIRKPPKF 510

BLAST of CsGy7G000670 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 3.2e-79
Identity = 203/515 (39.42%), Postives = 290/515 (56.31%), Query Frame = 0

Query: 32  RGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKAD 91
           + +++ +AT+  LQ   +   LTS ++V+ Y+    ++NP V+G++++NPD L  A + D
Sbjct: 62  QNITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELD 121

Query: 92  REREANKPGSLCG-LHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRKAG 151
            ER AN  G + G LHGIP ++KDN  TKDK++TTAGS+ALLGSIVPRDA VVK+LR+AG
Sbjct: 122 DER-AN--GIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAG 181

Query: 152 AIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVS 211
           A++ G A+LSEWAD RS     G SARGGQ + P+ L+ +P GSSSG +ISVA+N+ A +
Sbjct: 182 AVLFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFA 241

Query: 212 IGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTI 271
           +GTETDGSI+ PA  N VVG+KPTVGLTSR GVIP S  QDT GPI RTV DAV V  ++
Sbjct: 242 LGTETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSM 301

Query: 272 VGFDYNDAATRTASKYIPY-GGYKQFL-NPNGLKGKRLGIVRNPFFSFFNDSTITQAFED 331
            G D ND  T   +   P  G Y +FL N   L+G R G+     +       I +  E 
Sbjct: 302 WGIDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEIDRLLE- 361

Query: 332 HFNTLKQGGAILIDNLEIADIDII--------LNVTASGEAAALLAEFKQSLNGYLKELV 391
               +++ GAI+ +N    ++D+I        L      E   +  +F  ++  YL E+ 
Sbjct: 362 VVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVK 421

Query: 392 VSPVRSLADIIAFNNANADQE-----LLNVF--GQEIFLAAEATNGI-GDVQKAAVLNLG 451
            + + SL DI+ +NN     E     ++  F  GQ+ FLA+    G+  +    AV  + 
Sbjct: 422 NTEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVR 481

Query: 452 KLTED-GFEKLV-----KENQ---LDAVVTP-GTGIA-TVLAIGGFPGINVPAGYDGGGV 511
           + ++D G +  +     K N    L+ ++ P GT I     A  G+P I +P G    G 
Sbjct: 482 RTSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGR 541

Query: 512 PFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSF 517
           PFG+        E +LI+     E     +  P F
Sbjct: 542 PFGLGIMHSAWQEPQLIKYGSAIEDLLQYKAKPKF 572

BLAST of CsGy7G000670 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.2e-68
Identity = 177/488 (36.27%), Postives = 256/488 (52.46%), Query Frame = 0

Query: 44  LQLAFKQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLC 103
           LQ  ++Q  +    +V  Y+  I  +N  V  V EINPDAL  A + D ER   K G L 
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNER---KMGKLR 153

Query: 104 G-LHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEW 163
           G LHG+P+++K+NI T DK+++TAGS+A+ G+    DA V  +LR+AG +I+GK+  S+W
Sbjct: 154 GPLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQW 213

Query: 164 ADFRSLAAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCP 223
           A+FRSL +  G SA GGQ    Y+ +  P GSSSG  ++    +A  ++GTET GSI+ P
Sbjct: 214 ANFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSP 273

Query: 224 ASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRT 283
           A  +++VG+KPTVGLTSR  V+P+S RQDT+GP+ R+V DA  +L  I G D ND  T +
Sbjct: 274 ADKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-S 333

Query: 284 ASKYIPYGGYKQFLNPNGLKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILID 343
           A  +     Y +  + N LKGKR+G+ RN    F +  T+   F      +K+ GAI+++
Sbjct: 334 AIPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAIIVE 393

Query: 344 NLEIADIDIILNVTASGEAAALLAEFKQSLNGYLKELVVSP--VRSLADIIAFNNANADQ 403
           N +              +   L A+   +L  + K+L V+P  +  L  +  F   +  +
Sbjct: 394 NTDFTSFAEFAQSPIPDD--ILYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHHRLE 453

Query: 404 EL--LNVFGQEIFLAAEATNGIGDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGI 463
           E    +    +I L     N           N+    E G    ++ ++LDA V P    
Sbjct: 454 EYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTDLS 513

Query: 464 ATVLAIGGFPGINVPAGY---------------DGGGVPFGINFGGLKGSETKLIEVAYG 512
             + A+ G P I VP G                 G G+P GI F G   SE KLI +AY 
Sbjct: 514 PYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLAYA 573

BLAST of CsGy7G000670 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 8.6e-48
Identity = 158/506 (31.23%), Postives = 246/506 (48.62%), Query Frame = 0

Query: 34  LSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADRE 93
           + +   T+H+L+   K+ ++++ E+   Y+  I+ + P +  ++ I  D  LQ  KA   
Sbjct: 1   MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQ--KAKEA 60

Query: 94  REANKPGSLCGLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRKAGAII 153
            E  K G    L GIPV++KDNI T + + TT  S  L   I P +A VV++L + G II
Sbjct: 61  DEKIKKGEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVII 120

Query: 154 LGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGT 213
           LGK++L E+A   S    A  +      KNP+ LS  P GSS G + ++AA+ AA ++G+
Sbjct: 121 LGKSNLDEFAMGSSTENSAFKTT-----KNPWDLSRVPGGSSGGSAAAIAADEAAFALGS 180

Query: 214 ETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGF 273
           +T GSI  PAS   VVG+KPT GL SR G++  +   D IGP  + VTD  IVL+TI+G 
Sbjct: 181 DTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGH 240

Query: 274 DYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTL 333
           D  D+ +    K      Y  +L  + +KG R+G+ +  FF    +  + +  ++    L
Sbjct: 241 DPKDSTSLKIDK----PDYTSYLKED-IKGLRIGVAKE-FFGEGIEEGVKETVQESIKVL 300

Query: 334 KQGGAILIDNLEIADIDIIL---NVTASGEAAALLAEFKQSLNGYLKELVVSPVRSLADI 393
           +  GA +ID + I  ++  L    + AS EA++ LA +     G++ E          D+
Sbjct: 301 QDLGAEIID-ISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAE-------KYEDL 360

Query: 394 IAFNNANADQELLNVFGQEIFLAAEA-TNGIGDVQKAAVLNLGKLTEDGFEKLVKENQLD 453
           I        +       + I L   A ++G  D      L +  L ++ FEK  +  + D
Sbjct: 361 IDMYMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAFE--KCD 420

Query: 454 AVVTP-------------GTGIATVLA--------IGGFPGINVPAGYDGGGVPFGINFG 513
            ++ P                +A  LA        I G PGI++P G    G+P G+   
Sbjct: 421 VIIGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGL-SDGLPVGLQII 480

Query: 514 GLKGSETKLIEVAYGFEQATLIRKPP 515
           G    E K++ VAY FEQA      P
Sbjct: 481 GRHFDEGKILNVAYAFEQANKFSAKP 481

BLAST of CsGy7G000670 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.6e-47
Identity = 160/510 (31.37%), Postives = 248/510 (48.63%), Query Frame = 0

Query: 34  LSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADRE 93
           + +   T+H+L+   K+ ++++ E+   Y+  I+ + P +  +I I  D  LQ  +A   
Sbjct: 1   MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQ--RAKEA 60

Query: 94  REANKPGSLCGLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRKAGAII 153
            E  K G    L GIPV++KDNI T + + TT  S  L   I P +A VV++L + G II
Sbjct: 61  DEKIKNGEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVII 120

Query: 154 LGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGT 213
           LGK++L E+A   S    A  +      KNP+ LS  P GSS G + ++AA+ AA ++G+
Sbjct: 121 LGKSNLDEFAMGSSTENSAFKTT-----KNPWDLSRVPGGSSGGSAAAIAADEAAFALGS 180

Query: 214 ETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGF 273
           +T GSI  PAS   VVG+KPT GL SR G++  +   D IGP  + VTD  IVL+TI+G 
Sbjct: 181 DTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGH 240

Query: 274 DYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTL 333
           D  D+ +    K      Y  +L  + +KG R+G+ +  FF    +  + +  ++    L
Sbjct: 241 DPKDSTSLKIDK----PDYTSYLKED-IKGLRIGVAKE-FFGEGIEEGVKETVQESIKVL 300

Query: 334 KQGGAILIDNLEIADIDIIL---NVTASGEAAALLAEFKQSLNGYLKELVVSPVRSLADI 393
           +  GA +ID + I  ++  L    + AS EA++ LA +     G+    +      L D+
Sbjct: 301 QDLGAEIID-ISIPYVEYALPAYYIIASAEASSNLARYDGIRYGH----IAGKYEDLIDM 360

Query: 394 IAFNNANADQELLNVFGQE----IFLAAEA-TNGIGDVQKAAVLNLGKLTEDGFEKLVKE 453
                +         FG+E    I L   A ++G  D      L +  L ++ FEK  + 
Sbjct: 361 YMVTRSEG-------FGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAFE- 420

Query: 454 NQLDAVVTP-------------GTGIATVLA--------IGGFPGINVPAGYDGGGVPFG 513
            + D ++ P                +A  LA        I G PGI++P G    G+P G
Sbjct: 421 -KCDVIIGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGL-SDGLPVG 480

Query: 514 INFGGLKGSETKLIEVAYGFEQATLIRKPP 515
           +   G    E K++ VAY FEQA      P
Sbjct: 481 LQIIGRHFDEGKILNVAYAFEQANKFNAKP 481

BLAST of CsGy7G000670 vs. NCBI nr
Match: XP_031745278.1 (probable amidase At4g34880 [Cucumis sativus] >KGN43234.1 hypothetical protein Csa_020255 [Cucumis sativus])

HSP 1 Score: 996 bits (2576), Expect = 0.0
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0

Query: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60
           MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN
Sbjct: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480
           GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG
Sbjct: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518
           VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518

BLAST of CsGy7G000670 vs. NCBI nr
Match: KAA0049736.1 (putative amidase isoform X2 [Cucumis melo var. makuwa] >TYK12141.1 putative amidase isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 499/518 (96.33%), Postives = 509/518 (98.26%), Query Frame = 0

Query: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60
           MA+SSP SSVAFSLLLILVAFC T  + VTVRGLSIREATVHDLQLAFKQNQLTSRELVN
Sbjct: 1   MANSSPLSSVAFSLLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDAL+QAYKADREREANKPGSLCGLHGIP+LLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLRKAGAIILGKASLSEWADFRSL APAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDSTITQAF+DHFNTLKQGGAILIDNLEIA+I+IILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANINIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480
           GDVQKAA+LNLGKLTEDGFEKLVKENQLDAVVTPG+GIATVLAIGGFPGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKENQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518
           VPFGINFGGLKGSE KLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

BLAST of CsGy7G000670 vs. NCBI nr
Match: XP_016900445.1 (PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo])

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 498/518 (96.14%), Postives = 509/518 (98.26%), Query Frame = 0

Query: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60
           MA+SSP SSVAFS+LLILVAFC T  + VTVRGLSIREATVHDLQLAFKQNQLTSRELVN
Sbjct: 1   MANSSPLSSVAFSMLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDAL+QAYKADREREANKPGSLCGLHGIP+LLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLRKAGAIILGKASLSEWADFRSL APAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDSTITQAF+DHFNTLKQGGAILIDNLEIA+IDIILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480
           GDVQKAA+LNLGKLTEDGFEKLVK+NQLDAVVTPG+GIATVLAIGGFPGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKDNQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518
           VPFGINFGGLKGSE KLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

BLAST of CsGy7G000670 vs. NCBI nr
Match: XP_038887424.1 (probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 481/518 (92.86%), Postives = 491/518 (94.79%), Query Frame = 0

Query: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60
           MA SSP SSVAFSLLLILVAFC T  HS TVRGLSIREATV DLQLAFKQNQLTSR LV 
Sbjct: 1   MAYSSPLSSVAFSLLLILVAFCLTAPHSATVRGLSIREATVQDLQLAFKQNQLTSRRLVE 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120
           FYIGEI RLNPVVHGVIEINPDALLQAYKADRER A KPGSLCGLHGIPVLLKDNIGTKD
Sbjct: 61  FYIGEIHRLNPVVHGVIEINPDALLQAYKADRERAAKKPGSLCGLHGIPVLLKDNIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVP DAG+VKRLR+AGAIILGKASLSEWADFRSL APAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPHDAGIVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGP+ISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPAISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRT SKYIP GGYKQFLNP G
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTTSKYIPNGGYKQFLNPIG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDS IT+AFEDHFNTLKQGGAILIDNLEI +IDIILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSAITEAFEDHFNTLKQGGAILIDNLEIENIDIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           A ALLAEFKQSLN YLKELV SPVRSLADIIAFNNAN DQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AVALLAEFKQSLNEYLKELVASPVRSLADIIAFNNANPDQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480
           GDVQ AA+LNL KLTEDGFEK+VK+ +LDAVVTPG+GIATVLAIGGFPGINVPAGYDGGG
Sbjct: 421 GDVQTAALLNLAKLTEDGFEKVVKKKRLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518
           VPFGINFGGLKGSE KLIEVAYGFEQATLIRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 518

BLAST of CsGy7G000670 vs. NCBI nr
Match: XP_022968892.1 (putative amidase C869.01 [Cucurbita maxima])

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 466/511 (91.19%), Postives = 482/511 (94.32%), Query Frame = 0

Query: 8   SSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIR 67
           S    S LLI+VAFC T   S  VRG SIREATV DL LAFK+N+LTSR LV FYIGEIR
Sbjct: 16  SMACSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLHLAFKRNELTSRGLVEFYIGEIR 75

Query: 68  RLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKDKLNTTAG 127
           RLNPVVHGVIEINPDALLQAYKADREREA KPGSLCGLHGIPVLLKD+IGTKDKLNTTAG
Sbjct: 76  RLNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAG 135

Query: 128 SFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVL 187
           SFALLGSIVPRDAGVVKRLR+AGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVL
Sbjct: 136 SFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVL 195

Query: 188 SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS 247
           SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS
Sbjct: 196 SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS 255

Query: 248 PRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLG 307
           PRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRT+SKYIPYGGYKQFLN NGLKGKRLG
Sbjct: 256 PRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTSSKYIPYGGYKQFLNANGLKGKRLG 315

Query: 308 IVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGEAAALLAE 367
           IVRNPFFSFFNDS ITQAFEDHFN LKQGGA+L+DNLEIA+IDIILNVTASGEAAALLAE
Sbjct: 316 IVRNPFFSFFNDSAITQAFEDHFNILKQGGAVLVDNLEIANIDIILNVTASGEAAALLAE 375

Query: 368 FKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAA 427
           FKQSLN YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAA
Sbjct: 376 FKQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAA 435

Query: 428 VLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGGVPFGINF 487
           +LNL KLT+DGFEK+V+E +LDAVVTPG  IATVLAIGGFPGINVPAGYDGGGVPFGINF
Sbjct: 436 LLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINF 495

Query: 488 GGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518
           GGLKGSE KLIEVAY FEQATLIRKPPSFKP
Sbjct: 496 GGLKGSEPKLIEVAYAFEQATLIRKPPSFKP 526

BLAST of CsGy7G000670 vs. ExPASy TrEMBL
Match: A0A0A0K2I0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009780 PE=4 SV=1)

HSP 1 Score: 996 bits (2576), Expect = 0.0
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0

Query: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60
           MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN
Sbjct: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480
           GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG
Sbjct: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518
           VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518

BLAST of CsGy7G000670 vs. ExPASy TrEMBL
Match: A0A5A7U6F5 (Putative amidase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00780 PE=4 SV=1)

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 499/518 (96.33%), Postives = 509/518 (98.26%), Query Frame = 0

Query: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60
           MA+SSP SSVAFSLLLILVAFC T  + VTVRGLSIREATVHDLQLAFKQNQLTSRELVN
Sbjct: 1   MANSSPLSSVAFSLLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDAL+QAYKADREREANKPGSLCGLHGIP+LLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLRKAGAIILGKASLSEWADFRSL APAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDSTITQAF+DHFNTLKQGGAILIDNLEIA+I+IILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANINIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480
           GDVQKAA+LNLGKLTEDGFEKLVKENQLDAVVTPG+GIATVLAIGGFPGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKENQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518
           VPFGINFGGLKGSE KLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

BLAST of CsGy7G000670 vs. ExPASy TrEMBL
Match: A0A1S4DXK2 (putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490301 PE=4 SV=1)

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 498/518 (96.14%), Postives = 509/518 (98.26%), Query Frame = 0

Query: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60
           MA+SSP SSVAFS+LLILVAFC T  + VTVRGLSIREATVHDLQLAFKQNQLTSRELVN
Sbjct: 1   MANSSPLSSVAFSMLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDAL+QAYKADREREANKPGSLCGLHGIP+LLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLRKAGAIILGKASLSEWADFRSL APAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDSTITQAF+DHFNTLKQGGAILIDNLEIA+IDIILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480
           GDVQKAA+LNLGKLTEDGFEKLVK+NQLDAVVTPG+GIATVLAIGGFPGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKDNQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518
           VPFGINFGGLKGSE KLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

BLAST of CsGy7G000670 vs. ExPASy TrEMBL
Match: A0A6J1HUS8 (putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111468076 PE=4 SV=1)

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 466/511 (91.19%), Postives = 482/511 (94.32%), Query Frame = 0

Query: 8   SSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIR 67
           S    S LLI+VAFC T   S  VRG SIREATV DL LAFK+N+LTSR LV FYIGEIR
Sbjct: 16  SMACSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLHLAFKRNELTSRGLVEFYIGEIR 75

Query: 68  RLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKDKLNTTAG 127
           RLNPVVHGVIEINPDALLQAYKADREREA KPGSLCGLHGIPVLLKD+IGTKDKLNTTAG
Sbjct: 76  RLNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAG 135

Query: 128 SFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVL 187
           SFALLGSIVPRDAGVVKRLR+AGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVL
Sbjct: 136 SFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVL 195

Query: 188 SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS 247
           SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS
Sbjct: 196 SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS 255

Query: 248 PRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLG 307
           PRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRT+SKYIPYGGYKQFLN NGLKGKRLG
Sbjct: 256 PRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTSSKYIPYGGYKQFLNANGLKGKRLG 315

Query: 308 IVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGEAAALLAE 367
           IVRNPFFSFFNDS ITQAFEDHFN LKQGGA+L+DNLEIA+IDIILNVTASGEAAALLAE
Sbjct: 316 IVRNPFFSFFNDSAITQAFEDHFNILKQGGAVLVDNLEIANIDIILNVTASGEAAALLAE 375

Query: 368 FKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAA 427
           FKQSLN YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAA
Sbjct: 376 FKQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAA 435

Query: 428 VLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGGVPFGINF 487
           +LNL KLT+DGFEK+V+E +LDAVVTPG  IATVLAIGGFPGINVPAGYDGGGVPFGINF
Sbjct: 436 LLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINF 495

Query: 488 GGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518
           GGLKGSE KLIEVAY FEQATLIRKPPSFKP
Sbjct: 496 GGLKGSEPKLIEVAYAFEQATLIRKPPSFKP 526

BLAST of CsGy7G000670 vs. ExPASy TrEMBL
Match: A0A6J1GLV2 (putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111455539 PE=4 SV=1)

HSP 1 Score: 889 bits (2296), Expect = 0.0
Identity = 463/506 (91.50%), Postives = 478/506 (94.47%), Query Frame = 0

Query: 13  SLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPV 72
           S LLI+VAFC T   S  VRG SIREATV DLQLAFK+N+LTSR LV FYIGEIRRLNPV
Sbjct: 5   SWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPV 64

Query: 73  VHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKDKLNTTAGSFALL 132
           VHGVIEINPDALLQAYKADREREA KPGSLCGLHGIPVLLKD+IGTKDKLNTTAGSFALL
Sbjct: 65  VHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALL 124

Query: 133 GSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPC 192
           GSIVPRDAGVVKRLR+AGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPC
Sbjct: 125 GSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPC 184

Query: 193 GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 252
           GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT
Sbjct: 185 GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 244

Query: 253 IGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNP 312
           IGPIGRTVTDAVIVLDTIVGFDYND ATRT+SKYIPYGGYKQFLN NGLKGKRLGIVRNP
Sbjct: 245 IGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNP 304

Query: 313 FFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGEAAALLAEFKQSL 372
           FFSFFNDS ITQAFEDHFN LKQGGAIL+DNLEI +IDIILNVTASGEA ALLAEFKQSL
Sbjct: 305 FFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEFKQSL 364

Query: 373 NGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAAVLNLG 432
           N YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAA+LNL 
Sbjct: 365 NEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLA 424

Query: 433 KLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 492
           KLT+DGFEK+V+E +LDAVVTPG  IATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG
Sbjct: 425 KLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 484

Query: 493 SETKLIEVAYGFEQATLIRKPPSFKP 518
           SE  LIEVAY FEQATLIRKPPSFKP
Sbjct: 485 SEPTLIEVAYAFEQATLIRKPPSFKP 510

BLAST of CsGy7G000670 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 511.9 bits (1317), Expect = 5.9e-145
Identity = 282/512 (55.08%), Postives = 355/512 (69.34%), Query Frame = 0

Query: 12  FSLLLILVAFCSTPTHSVTVRG-------LSIREATVHDLQLAFKQNQLTSRELVNFYIG 71
           FS LLIL+        SV            SI+EAT+ D+++AF + +LTS++LV  Y+ 
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 72  EIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKDKLNT 131
            I +LNP++H VIE NPDAL+QA  ADRER+      L  LHG+PVLLKD+I TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 132 TAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNP 191
           TAGSFALLGS+V RDAGVVKRLR++GA+ILGKASLSEWA FRS + P G SA        
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA-------- 188

Query: 192 YVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVI 251
                                                  S NSVVGIKP+VGLTSRAGV+
Sbjct: 189 --------------------------------------PSQNSVVGIKPSVGLTSRAGVV 248

Query: 252 PVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGK 311
           P+S RQD+IGPI RTV+DAV +LD IVG+D  D AT+TAS++IP GGYKQFL  +GLKGK
Sbjct: 249 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 308

Query: 312 RLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGEAAAL 371
           RLGIV             +   + H  TL++ GAI+I+NL I +I++I+  T SGE  AL
Sbjct: 309 RLGIVMKH----------SSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIAL 368

Query: 372 LAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQ 431
           LAEFK SLN YLKELV SPVRSLAD+IA+N   A+QE +  +GQE+FL AEAT+G+G+ +
Sbjct: 369 LAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKE 428

Query: 432 KAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGGVPFG 491
           K A+  + +L+ +G EKL++EN+LDA+VT G+ +++VLAIGG+PGINVPAGYD GGVP+G
Sbjct: 429 KTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYG 464

Query: 492 INFGGLKGSETKLIEVAYGFEQATLIRKPPSF 517
           I+FGGL+ SE KLIE+A+ FEQATLIRKPP F
Sbjct: 489 ISFGGLRFSEPKLIEIAFAFEQATLIRKPPKF 464

BLAST of CsGy7G000670 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 124.4 bits (311), Expect = 2.7e-28
Identity = 143/537 (26.63%), Postives = 241/537 (44.88%), Query Frame = 0

Query: 12  FSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNP 71
           F +   +V+  S+ T   +V  +S  ++ +   + +    + T+ E+   Y+  IR   P
Sbjct: 19  FQISKTIVSAASSKTIDTSV--ISPPQSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEP 78

Query: 72  VVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKDKLNTTAGSFAL 131
            +   + ++ + L  A + D  +   K   L  L G+ + +KDNI T+  + +TA S  L
Sbjct: 79  QLKCFLHVSENVLKDAQEID--QRIAKGEELGPLAGVLIGVKDNICTQG-MPSTAASRIL 138

Query: 132 LGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASP 191
                P DA  VK++++ G I++GK ++ E+    +  A A          NP+ LS  P
Sbjct: 139 EHYRPPFDATAVKKIKELGGIVVGKTNMDEFGMGSTTEASAFQVT-----ANPWDLSRVP 198

Query: 192 CGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQD 251
            GSS G + +VAA    VS+G++T GS+  PASF  VVG+KPT G  SR G++  +   D
Sbjct: 199 GGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLD 258

Query: 252 TIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLN-----PNGLKGKRL 311
            IG  G TV DA ++L  I G+D  D+   T+SK        QFL+        L G ++
Sbjct: 259 VIGCFGSTVADAGMLLHAISGYDRFDS---TSSKQDVPEFQSQFLSVDHFESKPLNGVKV 318

Query: 312 GIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIIL---NVTASGEAAA 371
           GI+R        DS +  A ++  + L+  G IL + + +    + L    V AS E+++
Sbjct: 319 GIIRETLEDGV-DSGVRSATQEAASHLEALGCILTE-VSLPSFSLGLPAYYVIASSESSS 378

Query: 372 LLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDV 431
            L+ +     G   +++   +  L +         + ++  + G     A     G  D 
Sbjct: 379 NLSRYDGVRYG--NQVMAEELNKLYECSRGEGFGGEVKMRILMGTYALSA-----GYYDA 438

Query: 432 QKAAVLNLGKLTEDGFEKLVKENQL------------------DAVVTPGTGIATV-LAI 491
                  +  L    F+  +++N +                  D +      I TV + +
Sbjct: 439 YYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVNL 498

Query: 492 GGFPGINVPAG-YDGG--GVPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 519
            G P + +P G  +GG  G+P G+   G    E KL++V + FEQ     K  SF P
Sbjct: 499 AGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTL---KGSSFVP 530

BLAST of CsGy7G000670 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 117.1 bits (292), Expect = 4.3e-26
Identity = 82/252 (32.54%), Postives = 132/252 (52.38%), Query Frame = 0

Query: 40  TVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPD-ALLQAYKADREREANK 99
           +V +L    K  Q+TS+ELV  Y+ +++R N V+  V+    + A  QA +AD   +   
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD---DLLS 263

Query: 100 PGSLCG-LHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKA 159
            G+  G LHGIP  LKD +       TT GS +     +  +A V KRL+ +GA+++ K 
Sbjct: 264 QGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKL 323

Query: 160 SLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDG 219
                A +  +         GG+ +NP+ +     GSS+GP+ S +A +   +IG+ET G
Sbjct: 324 VTGSMA-YDDIWF-------GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAG 383

Query: 220 SILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYND 279
           S+  PA+   +  ++PT G   R GV+ +S   D +GP  RT  D  ++LD I G D +D
Sbjct: 384 SMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 443

Query: 280 AATRTASKYIPY 290
            ++R  +   P+
Sbjct: 444 LSSREIAFEDPF 443

BLAST of CsGy7G000670 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 109.4 bits (272), Expect = 8.9e-24
Identity = 81/252 (32.14%), Postives = 129/252 (51.19%), Query Frame = 0

Query: 40  TVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPD-ALLQAYKADREREANK 99
           +V +L    K  Q+TS+ELV  Y+ +++R N V+  V+    + A  QA +AD   +   
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD---DLLS 263

Query: 100 PGSLCG-LHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKA 159
            G+  G LHGIP  LKD +       TT GS +     +  +A V KRL+ +GA+++ K 
Sbjct: 264 QGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKL 323

Query: 160 SLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDG 219
                A +  +         GG+ +NP+ +     GSS+GP+       A+ S G+ET G
Sbjct: 324 VTGSMA-YDDIWF-------GGRTRNPWNIEEFSTGSSAGPA-------ASTSAGSETAG 383

Query: 220 SILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYND 279
           S+  PA+   +  ++PT G   R GV+ +S   D +GP  RT  D  ++LD I G D +D
Sbjct: 384 SMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 436

Query: 280 AATRTASKYIPY 290
            ++R  +   P+
Sbjct: 444 LSSREIAFEDPF 436

BLAST of CsGy7G000670 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 95.5 bits (236), Expect = 1.3e-19
Identity = 126/529 (23.82%), Postives = 218/529 (41.21%), Query Frame = 0

Query: 3   DSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFY 62
           D SP   +  +L  +     S   H+  V   S R   + D   A++    T  ++    
Sbjct: 95  DESPIDRLETALKCLPQYDPSRSLHADPVS--SFRYWKIRDYAYAYRSKLTTPLQVAKRI 154

Query: 63  IGEIRRL---NPVVHGVIEINPDALL-QAYKADREREANKPGSLCGLHGIPVLLKDNIGT 122
           I  I       P    +I  + + ++ QA  + R  E   P S+  L GI V +KD+I  
Sbjct: 155 ISIIEEFGYDKPPTPFLIRFDANEVIKQAEASTRRFEQGNPISV--LDGIFVTIKDDIDC 214

Query: 123 KDKLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARG 182
                    ++      V +D+ VV +LR  GAI+LGKA++ E           G ++  
Sbjct: 215 LPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG-----MGTTGNNSNY 274

Query: 183 GQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLT 242
           G  +NP+       GSSSG +  VAA + + ++GT+  GS+  P++   + G+K T G T
Sbjct: 275 GTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRT 334

Query: 243 SRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNP 302
              G +      + IGP+  ++ DA +V   I+G   + +A R   K  P   + + L+ 
Sbjct: 335 DMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILG---SSSADRYNLKPSP-PCFPKLLSH 394

Query: 303 NGLKGKRLGIVR-NPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTA 362
           NG     +G +R   +  +FND + +   +   + LK    +L +N     ++I++    
Sbjct: 395 NG--SNAIGSLRLGKYTKWFNDVSSSDISDKCEDILK----LLSNNHGCKVVEIVVPELE 454

Query: 363 SGEAAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEA- 422
              AA +++    +L+             L+      +      +   F    ++AA+  
Sbjct: 455 EMRAAHVISIGSPTLSSLTPYCEAGKNSKLS-----YDTRTSFAIFRSFSASDYIAAQCL 514

Query: 423 --------TNGIGDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIG--G 482
                    N   DV        G          +K  + +  VT    +  VLA    G
Sbjct: 515 RRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDALKNGETNIQVTTDL-MRFVLAANLLG 574

Query: 483 FPGINVPAGYDGGGVPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPS 516
           FP I+VP GYD  G+P G+   G   +E  ++ +A   E+   + K P+
Sbjct: 575 FPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGLAAAVEELAPVTKKPA 598

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1P8B7606.8e-17062.30Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY43.2e-7939.42Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C81.2e-6836.27Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
B0K3S38.6e-4831.23Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain ... [more]
B0KBN45.6e-4731.37Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanol... [more]
Match NameE-valueIdentityDescription
XP_031745278.10.0100.00probable amidase At4g34880 [Cucumis sativus] >KGN43234.1 hypothetical protein Cs... [more]
KAA0049736.10.096.33putative amidase isoform X2 [Cucumis melo var. makuwa] >TYK12141.1 putative amid... [more]
XP_016900445.10.096.14PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo][more]
XP_038887424.10.092.86probable amidase At4g34880 [Benincasa hispida][more]
XP_022968892.10.091.19putative amidase C869.01 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0K2I00.0100.00Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009780 PE=... [more]
A0A5A7U6F50.096.33Putative amidase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S4DXK20.096.14putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490301 PE=4... [more]
A0A6J1HUS80.091.19putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111468076 PE=4 SV=1[more]
A0A6J1GLV20.091.50putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111455539 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G34880.15.9e-14555.08Amidase family protein [more]
AT3G25660.12.7e-2826.63Amidase family protein [more]
AT5G07360.14.3e-2632.54Amidase family protein [more]
AT5G07360.28.9e-2432.14Amidase family protein [more]
AT5G64440.11.3e-1923.82fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 57..460
e-value: 2.3E-69
score: 234.6
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 19..516
e-value: 3.8E-142
score: 476.8
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 35..515
NoneNo IPR availablePANTHERPTHR42678:SF19C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 25..518
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 25..518

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy7G000670.1CsGy7G000670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane