CsGy6G036395 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G036395
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionfatty acid hydroxylase 2
LocationGy14Chr6: 31905289 .. 31909449 (+)
RNA-Seq ExpressionCsGy6G036395
SyntenyCsGy6G036395
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTATTGTTTTTCTAGTGTAAACAAAAGTGAGAAATTCAAAGAATAAACTTGTTGGGTCATCAACACATAGTAGCAAGCAAAATGAACCATGAAAGATATAAGGGGTCAAAAGGGAAAGTGATTGAGGAAAATTTTGGAGGAAGGTCATCTCAATTTCATGGATTTAGAGTTGAAATTTTAATAGGGTTAAAGAAGAGAATTTATTAAATGGTTGATGAGAAACTTTTGATTTGACTCATCCACTTAATTAGAGGGGTTAAAGGTAGAAGTTTTTAGTTGAAAAAGAAGAAAGGAGGGATATTTTTGTTTATGGGTTTCTATTTTTTTGAAGTACTAAACCGAGAGGGGAAATTGGGAAAATGCAGGACAGTGTGGATTTGTGAGGCCAAGCAAGGATTTCGATTCCAATAGAGTGGGTGTTTTTGAGCCTCCCAATTTCAACATAAATCCCTCCATTCTTCTTCTCTAATATTTGGCTTTCTTTATTTCTTTCTGTTCTTTCACGGGTTTCTCTTACGTATAGATCTGAACATTCCTTCAGATCTCGTGATACGGCTGGAGGAGCACATTCTCGGACAAGCCCAATTTCATCTTCCTCGAAATCAATCCCTTCTCGGGTTTCTCCATCTCCACTGCATTGTGCGGAAAAGGTTGGTATTCGGGAGTCGGGTGAATCTTTTTATCTACCTTCAGATTTCTTCTCTTTTTCTTAATTCGAGTCAGTGAGGGTGGAATTTTTAATATCTCGATTTCTTTATTTAATTTCTCATCTGGGTATTGTTCTCCAACTTCGGATGTCACAATATTCTGTTCCACACTCTAGCTCATTGTGTGATGTTGTGCTAATTGGGATGCCATTGTCTTGATTTCTTCTTTATTGGGTTCTGGGTATTCTCTACGTTCTTTCTTAAATCTGTTGATTTAGGTTCTTTAGGTTCAACGGTGAATTCTTCTTGTGTTTTTGTGTTCTACTTCTTTCTTTGCTATTTTGGGTCCTCAAATCCAATGCGGGTATATTTTCAGCTTACGCTTAGTAAAACTACAACTTAGTCCTTTTTTTTGGATTTGAGAAATGATTTGAATTGATCTGCTATTTACGAAAATCTAATCATTCTAATTTGGTTAGTTAAATGGTGATATTGCTTTGCTTGGAGCATCAAGTTTGCGAAATATATAATTATGCCTTTTAGTCATATAGGCTCTTTTCGCTCAAATAAATTAACTTGTCACAACACATAAGATTGCCAGCATATGCAAAGTCACATCATTCTTTAGTTTGTTAAGTTTAGTTAATTGCGGAACAATGATTTCGGAGACTAGTTTTAAACATTTCGCTTTTTAGAATAGGTGACAGAAAACAAATATTTCTTTTAAACTTGTGGGTGTTATTGTATTCAATTTTGTATCCTTTGTCGTAAGCTTTCTTATCTGCCCAAGTGCATTTCTCTGTACTGAATAAAATTATATCTCACGAGTAAGGGGGAGGCAAAATGTTTAAGCTGTTGAAAGTCGTTTGAATTGTCCTATTTCAACTGTTCCATTATGTGATGTTTATTTTCTTACTGTTGGCTAACTTTCGTATTTTTCATTGTCCTGTATGCAAAAGATTAATTTCGAAGCCCCTCAGTAGAAGATTCCAAGATGTCTTCAAAGCCTTTTACTGTAGATTTAAACAAGCCTCTTGTTTTTCAGGTTAGCCTCCTCCTTTTCTACTCCATACGTTTTCTGTTATTTCCTATAAAGTTGATATCATAAACATCATAGGAACTGAGCTTGCAAATGCAAATAAGTCGCTGTAATCTAGGCCCTTAAAGTTGTAATCCTTTTGAAAAAATCTCCCAACCTTTATGTTTCGAGGGTCAAGAAGTATAGCTGTACATTCTTGTCTTGATTACATTTAATCTATTCTAAGTTGTTTGGTTGTACTCTTCATAGTTGAATTGTAATCTTCTCTGCCATTTAGTCTTATTTAAATGTTTCTCTTTGTAAGTAATGGATTTTTTTTACAGGTTGGCCATCTTGGAGAAGCTTACAATGAATGGGTTCACCAGCCTATAATTAGCAAGGACGGCCCTCGATTCTTCGGAAATGATTTTCTGGAGGTTTTATTTGACTCTTTATAATAATTATATCCTGTTACACAACCTTAAGCATGAAGAGATAAACACAATATTGTTTGTCATAGGTGCAATAAACTCTCTTGGTGGTTATACCAGCAATTTTATCTAATAACATGCTCATTAATAATGTGGTGCAGTGTTTTTTAAAATTCTGCTCACAATTTGAAATATCAATGAAGAGGAGTATACTAAAACATTCAGATATTAATATAGCAACATCTCTTTTTGGAAATCAATCTGTTATATCTTTTCCCTTGGGACAAATAACTTATCGTATCCTGTGCTTACTACCATGGAGATGTCATCAAATAAAGTTACTATTCATACATTGATGTGATGTTTAACATATAACTTTTCTGAAATGACTAATGAGAGGCTTGGCTTTAATTTCTTTGTAATTACAGATGTTGACCCGAACAGTATGGTGGGTAATTCCACTTGTTTGGCTGCCAGTGATTTCCTGGTTGGTTTCTGTGTCTTTGTCGAGGGGCCTTACTCCCTCTGATGCAGCTTCATGTTTGGCTGGTGGGATTTTCATCTGGACCTTGCTTGAGTACACATTGCACCGCTTTCTTTTCCACATGGAAACAAGAAGCTACTGGTACTCTCTCATCTCTCTACACACACAGATAAATGGTAAATTCAAGGACACTAATGATATCACAACCTATTTCAGGGCAAACACTCTGCACTATGTACTTCATGGTTGTCATCACAAGCATCCGATGGATGGACTCAGACTCGTCTTCCCACCTGCTGCAACAACTATCTTGAGTGTGCCGGTAAGTTTGGAAAACGCTTACGTCTGAGATTATCCTCCGCAAATTTGCATGATAATCATGTGCTTACTGTTATATGTTATTTGAGGAAATTCTTTTTGTTGCAGTTTACATTTCCTCATGCTATGAATTAATGCTATGCACTGTTTCATGGAACCACCCTAAACGTTGGTTTTATATCCAATCAGCATGAGTGAACTACATTGCCATATGGTTTTAAAAGAGAAACCTTAAGGATTTTAATTGCATATTGATTGACCATTGACCTCAACACTTCACATGTTCCATATAGATAGATTTATGGATTCTGAAAGATGGAATGCATTGGCAATCATAAATGTTACTCCTAAAAAACGGTCGAATGAAATAATCTGTGTAGTATTGTGTGCAATCTAAGTAGCCAATCATCAAGTTCCAGGCTTATGTCATTAACTCAAGGGATTATGTGGTTTCTTGTCTTCTTTCTTATGGAGCCGTCTTTGTGATCTGTGTCTATCAGTTGTGGATCGTGATTAGACTTACATCGACTCCGGCTGTAGCTCCTGCTTTGTTTGGAGGTGGGTTGCTGGGATATGTAATGTACGACGTAACTCATTATTACCTGCATCATGGAAAGCCATCCCCAGGGTTATCTCAAAATTTGAAGGTAAAGTTGAAGGAATAATAAGACTGTGTTTGTTCTTGGAGAATTGAGTAAGGAAAGATTTCTTATGATTGTTATGTTGTGTTAAACAGAGATATCACTTGAACCATCACTTCAGAGATCAAAGTGAGGGATTTGGAATCACTTCTCCATTATGGGACAGAGCATTTGGAACTTATCCTACAACTAAATCCACTCAGAAAAACAAATAATGATGTTGGTTGCACAGATATTTAAGTCATCACCAGATTTATGTATTGAGTTTTGTTCTTCCTTTCTTTCTTTCTTTTTGTACATTGAGCATCAAATTCATGCTTCGACAACCATTATATATCTTTCTTTGGGGGTGATAGCATATGCCTTGTTTGAATATTGTATTGTTCTCCCTTCCCTCTGGTTATCAGATGTAGTAAGTTATTTTCATGTATATTAGCCATGGAGCTACACTTTGAATGAGGATTTCGTGTTTTTCAGTTTGCACATTGCAAAGCTCATACGTTTCTCTCTCTAATCTGTTTTCCCACGCACTTTGAATCTTCTCAAGTATAATATATTACCAACAAGGTTATGAGATTTGTTTAATTATCAGTGTTGTTAAAGGCAAATTAATTTAGA

mRNA sequence

ATGGTTATTGTTTTTCTAGTGTAAACAAAAGTGAGAAATTCAAAGAATAAACTTGTTGGGTCATCAACACATAGTAGCAAGCAAAATGAACCATGAAAGATATAAGGGGTCAAAAGGGAAAGTGATTGAGGAAAATTTTGGAGGAAGGTCATCTCAATTTCATGGATTTAGAGTTGAAATTTTAATAGGGTTAAAGAAGAGAATTTATTAAATGGTTGATGAGAAACTTTTGATTTGACTCATCCACTTAATTAGAGGGGTTAAAGGTAGAAGTTTTTAGTTGAAAAAGAAGAAAGGAGGGATATTTTTGTTTATGGGTTTCTATTTTTTTGAAGTACTAAACCGAGAGGGGAAATTGGGAAAATGCAGGACAGTGTGGATTTGTGAGGCCAAGCAAGGATTTCGATTCCAATAGAATCTCGTGATACGGCTGGAGGAGCACATTCTCGGACAAGCCCAATTTCATCTTCCTCGAAATCAATCCCTTCTCGGGTTTCTCCATCTCCACTGCATTGTGCGGAAAAGATTAATTTCGAAGCCCCTCAGTAGAAGATTCCAAGATGTCTTCAAAGCCTTTTACTGTAGATTTAAACAAGCCTCTTGTTTTTCAGGTTGGCCATCTTGGAGAAGCTTACAATGAATGGGTTCACCAGCCTATAATTAGCAAGGACGGCCCTCGATTCTTCGGAAATGATTTTCTGGAGATGTTGACCCGAACAGTATGGTGGGTAATTCCACTTGTTTGGCTGCCAGTGATTTCCTGGTTGGTTTCTGTGTCTTTGTCGAGGGGCCTTACTCCCTCTGATGCAGCTTCATGTTTGGCTGGTGGGATTTTCATCTGGACCTTGCTTGAGTACACATTGCACCGCTTTCTTTTCCACATGGAAACAAGAAGCTACTGGGCAAACACTCTGCACTATGTACTTCATGGTTGTCATCACAAGCATCCGATGGATGGACTCAGACTCGTCTTCCCACCTGCTGCAACAACTATCTTGAGTGTGCCGTTGTGGATCGTGATTAGACTTACATCGACTCCGGCTGTAGCTCCTGCTTTGTTTGGAGGTGGGTTGCTGGGATATGTAATGTACGACGTAACTCATTATTACCTGCATCATGGAAAGCCATCCCCAGGGTTATCTCAAAATTTGAAGAGATATCACTTGAACCATCACTTCAGAGATCAAAGTGAGGGATTTGGAATCACTTCTCCATTATGGGACAGAGCATTTGGAACTTATCCTACAACTAAATCCACTCAGAAAAACAAATAATGATGTTGGTTGCACAGATATTTAAGTCATCACCAGATTTATGTATTGAGTTTTGTTCTTCCTTTCTTTCTTTCTTTTTGTACATTGAGCATCAAATTCATGCTTCGACAACCATTATATATCTTTCTTTGGGGGTGATAGCATATGCCTTGTTTGAATATTGTATTGTTCTCCCTTCCCTCTGGTTATCAGATGTAGTAAGTTATTTTCATGTATATTAGCCATGGAGCTACACTTTGAATGAGGATTTCGTGTTTTTCAGTTTGCACATTGCAAAGCTCATACGTTTCTCTCTCTAATCTGTTTTCCCACGCACTTTGAATCTTCTCAAGTATAATATATTACCAACAAGGTTATGAGATTTGTTTAATTATCAGTGTTGTTAAAGGCAAATTAATTTAGA

Coding sequence (CDS)

ATGTCTTCAAAGCCTTTTACTGTAGATTTAAACAAGCCTCTTGTTTTTCAGGTTGGCCATCTTGGAGAAGCTTACAATGAATGGGTTCACCAGCCTATAATTAGCAAGGACGGCCCTCGATTCTTCGGAAATGATTTTCTGGAGATGTTGACCCGAACAGTATGGTGGGTAATTCCACTTGTTTGGCTGCCAGTGATTTCCTGGTTGGTTTCTGTGTCTTTGTCGAGGGGCCTTACTCCCTCTGATGCAGCTTCATGTTTGGCTGGTGGGATTTTCATCTGGACCTTGCTTGAGTACACATTGCACCGCTTTCTTTTCCACATGGAAACAAGAAGCTACTGGGCAAACACTCTGCACTATGTACTTCATGGTTGTCATCACAAGCATCCGATGGATGGACTCAGACTCGTCTTCCCACCTGCTGCAACAACTATCTTGAGTGTGCCGTTGTGGATCGTGATTAGACTTACATCGACTCCGGCTGTAGCTCCTGCTTTGTTTGGAGGTGGGTTGCTGGGATATGTAATGTACGACGTAACTCATTATTACCTGCATCATGGAAAGCCATCCCCAGGGTTATCTCAAAATTTGAAGAGATATCACTTGAACCATCACTTCAGAGATCAAAGTGAGGGATTTGGAATCACTTCTCCATTATGGGACAGAGCATTTGGAACTTATCCTACAACTAAATCCACTCAGAAAAACAAATAA

Protein sequence

MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPLVWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHYVLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK*
Homology
BLAST of CsGy6G036395 vs. ExPASy Swiss-Prot
Match: O48916 (Dihydroceramide fatty acyl 2-hydroxylase FAH1 OS=Arabidopsis thaliana OX=3702 GN=FAH1 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 2.5e-95
Identity = 152/237 (64.14%), Postives = 186/237 (78.48%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M ++ FTVDL KPLVFQVGHLGE Y EWVHQPI +K+GPRFF +DF E LT TVWW +P+
Sbjct: 1   MVAQGFTVDLKKPLVFQVGHLGEDYEEWVHQPIATKEGPRFFQSDFWEFLTLTVWWAVPV 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           +WLPV+ W +S S+S G +  +    +  GIFIWT  EY LHRF+FH++T+SYW NT HY
Sbjct: 61  IWLPVVVWCISRSVSMGCSLPEIVPIVVMGIFIWTFFEYVLHRFVFHIKTKSYWGNTAHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           ++HGCHHKHPMD LRLVFPP AT IL  P W + +  STP+ APALFGGG+LGYVMYDVT
Sbjct: 121 LIHGCHHKHPMDHLRLVFPPTATAILCFPFWNIAKAISTPSTAPALFGGGMLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 238
           HYYLHH +P+  +++NLK+YHLNHHFR Q +GFGITS LWD  FGT PTTK+ +K +
Sbjct: 181 HYYLHHAQPTRPVTKNLKKYHLNHHFRIQDKGFGITSSLWDIVFGTLPTTKAPRKEQ 237

BLAST of CsGy6G036395 vs. ExPASy Swiss-Prot
Match: Q9SUC5 (Dihydroceramide fatty acyl 2-hydroxylase FAH2 OS=Arabidopsis thaliana OX=3702 GN=FAH2 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 2.5e-95
Identity = 153/235 (65.11%), Postives = 186/235 (79.15%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M ++ +TVDLNKPLVFQVGHLGE Y EW+HQPI+  +GPRFF +DF E LTRTVWW IP 
Sbjct: 1   MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPT 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           +WLPV+ +++S+S S+GLT       +A G+  WTLLEYTLHRFLFH++T+SYWANT HY
Sbjct: 61  IWLPVVCYVLSISASKGLTFPQIGLIVAFGVLTWTLLEYTLHRFLFHIQTKSYWANTAHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           +LHGCHHKHP DGLRLVFPP AT IL VPLW ++ L +TPA APA+ GG L GYVMYD+T
Sbjct: 121 LLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATPATAPAILGGILFGYVMYDIT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQK 236
           HYYLHHG+P     ++LK+YHLNHHFR Q +G+GITS LWD+ FGT P  K+  K
Sbjct: 181 HYYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGTLPGIKAAAK 235

BLAST of CsGy6G036395 vs. ExPASy Swiss-Prot
Match: Q2LAM0 (Fatty acid 2-hydroxylase OS=Rattus norvegicus OX=10116 GN=Fa2h PE=1 SV=2)

HSP 1 Score: 212.2 bits (539), Expect = 6.3e-54
Identity = 110/241 (45.64%), Postives = 149/241 (61.83%), Query Frame = 0

Query: 8   VDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPLVWLPVIS 67
           VD  KPL++QVGHLGE Y+EWVHQP+      R F +D +E  ++TVW+ +P++W+P++ 
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPVARP--IRLFHSDLIEAFSKTVWYSVPIIWVPLVL 183

Query: 68  WLVSVSLSRGLTPSD---------------AASCLAG----GIFIWTLLEYTLHRFLFHM 127
           +L S S  R LT  +                 S   G    G+ IWTL+EY +HRFLFHM
Sbjct: 184 YL-SWSYYRTLTQDNIRLFASFTRDYSLVVPESVFIGLFVLGMLIWTLVEYLIHRFLFHM 243

Query: 128 E--TRSYWANTLHYVLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPAL 187
           +  + S++   LH+V+HG HHK P DG RLVFPP   +++    ++ +RL    AVA  L
Sbjct: 244 KPPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASVVVAFFYVFLRLILPEAVAGIL 303

Query: 188 FGGGLLGYVMYDVTHYYLHHGKPSPG-LSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFG 227
           F GGLLGYV+YD+THYYLH G P  G    N+K +H+ HHF  Q  GFGI++ LWD  F 
Sbjct: 304 FAGGLLGYVLYDMTHYYLHFGSPHKGSYLYNMKAHHVKHHFEYQKSGFGISTKLWDYFFH 361

BLAST of CsGy6G036395 vs. ExPASy Swiss-Prot
Match: Q4R4P4 (Fatty acid 2-hydroxylase OS=Macaca fascicularis OX=9541 GN=FA2H PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.4e-53
Identity = 109/240 (45.42%), Postives = 149/240 (62.08%), Query Frame = 0

Query: 8   VDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPLVWLPVIS 67
           VD  KPL++QVGHLGE Y+EWVHQP+      R F +D +E L++TVW+ +P++W+P++ 
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPVTRP--IRLFHSDLIEGLSKTVWYSVPIIWVPLVL 183

Query: 68  WLV----------SVSLSRGLTPSDA----ASCLAG----GIFIWTLLEYTLHRFLFHME 127
           +L           +V L    T   A     S   G    GIF+W+L+EY +HRFLFHM+
Sbjct: 184 YLSWSYYRTFAQGNVRLFTSFTTEYALAVPKSMFPGLFMLGIFLWSLIEYLIHRFLFHMK 243

Query: 128 --TRSYWANTLHYVLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALF 187
             + SY+   LH+V+HG HHK P DG RLVFPP   +++    ++ ++L    AV   +F
Sbjct: 244 PPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVIGVFYLCLQLILPEAVGGTVF 303

Query: 188 GGGLLGYVMYDVTHYYLHHGKPSPG-LSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGT 227
            GGLLGYV+YD+THYYLH G P  G    NLK +H+ HHF  Q  GFGI++ LWD  F T
Sbjct: 304 AGGLLGYVLYDMTHYYLHFGSPHRGSYLYNLKAHHVKHHFAHQKSGFGISTKLWDYCFHT 361

BLAST of CsGy6G036395 vs. ExPASy Swiss-Prot
Match: Q7L5A8 (Fatty acid 2-hydroxylase OS=Homo sapiens OX=9606 GN=FA2H PE=1 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 5.3e-53
Identity = 107/251 (42.63%), Postives = 151/251 (60.16%), Query Frame = 0

Query: 8   VDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPLVWLPVIS 67
           VD  KPL++QVGHLGE Y+EWVHQP+      R F +D +E L++TVW+ +P++W+P++ 
Sbjct: 124 VDWRKPLLWQVGHLGEKYDEWVHQPVTRP--IRLFHSDLIEGLSKTVWYSVPIIWVPLVL 183

Query: 68  WL--------------VSVSLSRGLTPSDAASCLAG----GIFIWTLLEYTLHRFLFHME 127
           +L              +  S +   T +   S   G    G F+W+L+EY +HRFLFHM+
Sbjct: 184 YLSWSYYRTFAQGNVRLFTSFTTEYTVAVPKSMFPGLFMLGTFLWSLIEYLIHRFLFHMK 243

Query: 128 --TRSYWANTLHYVLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALF 187
             + SY+   LH+V+HG HHK P DG RLVFPP   +++    ++ ++L    AV   +F
Sbjct: 244 PPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVIGVFYLCMQLILPEAVGGTVF 303

Query: 188 GGGLLGYVMYDVTHYYLHHGKPSPG-LSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGT 238
            GGLLGYV+YD+THYYLH G P  G    +LK +H+ HHF  Q  GFGI++ LWD  F T
Sbjct: 304 AGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFHT 363

BLAST of CsGy6G036395 vs. NCBI nr
Match: XP_004134612.1 (dihydroceramide fatty acyl 2-hydroxylase FAH2 [Cucumis sativus] >KGN49479.1 hypothetical protein Csa_003896 [Cucumis sativus])

HSP 1 Score: 502 bits (1293), Expect = 9.05e-180
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT
Sbjct: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237
           HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK
Sbjct: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237

BLAST of CsGy6G036395 vs. NCBI nr
Match: XP_008439533.1 (PREDICTED: dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Cucumis melo] >KAA0052473.1 dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Cucumis melo var. makuwa] >TYK13349.1 dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Cucumis melo var. makuwa])

HSP 1 Score: 498 bits (1283), Expect = 3.03e-178
Identity = 234/237 (98.73%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M+SKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISK+GPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           +LHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237
           HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK
Sbjct: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237

BLAST of CsGy6G036395 vs. NCBI nr
Match: XP_038881178.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like isoform X1 [Benincasa hispida] >XP_038881179.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 476 bits (1224), Expect = 3.01e-169
Identity = 221/237 (93.25%), Postives = 230/237 (97.05%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M+SKPFTVDLNKPLVFQVGHLGEAYNEWVHQPI+SK+GPRFFGNDF+EMLTRTVWWVIPL
Sbjct: 1   MASKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIVSKEGPRFFGNDFMEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGG+FIWTLLEYTLHRFLFHM+T SYW NTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGVFIWTLLEYTLHRFLFHMKTSSYWGNTLHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           +LHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRL STPAVAP+LFGGGLLGYVMYDVT
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLASTPAVAPSLFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237
           HYYLHHGKPS GLSQNLKRYHLNHHFR QSEGFGITSPLWDRAFGTYPTTK  QK+K
Sbjct: 181 HYYLHHGKPSSGLSQNLKRYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 237

BLAST of CsGy6G036395 vs. NCBI nr
Match: XP_023544587.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo] >XP_023544588.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 470 bits (1209), Expect = 5.83e-167
Identity = 217/237 (91.56%), Postives = 229/237 (96.62%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M+SK FTVDLNKPLVFQVGHLGEAYN+WVHQPI+SK+GPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKTFTVDLNKPLVFQVGHLGEAYNKWVHQPIVSKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           VWLPVISWLVSVSLSRGLTPSD ASCLAGG+FIW+LLEYTLHRFLFHMETRSYW NTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDVASCLAGGVFIWSLLEYTLHRFLFHMETRSYWGNTLHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           +LHGCHHKHPMDGLRLVFPPAAT ILSVPLW+VIRLTSTPAVAPALFGGGLLGYVMYDVT
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPAATAILSVPLWVVIRLTSTPAVAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237
           HYYLHHGKP+ GLSQNLKRYHLNHHFR QS+GFGITSPLWDRAFGTYP+TK  Q+NK
Sbjct: 181 HYYLHHGKPTSGLSQNLKRYHLNHHFRIQSQGFGITSPLWDRAFGTYPSTKPIQENK 237

BLAST of CsGy6G036395 vs. NCBI nr
Match: XP_022146704.1 (dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Momordica charantia] >XP_022146706.1 dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Momordica charantia] >XP_022146707.1 dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Momordica charantia])

HSP 1 Score: 470 bits (1209), Expect = 5.83e-167
Identity = 215/237 (90.72%), Postives = 227/237 (95.78%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M+SKP+TVDLNKPLVFQVGHLGEAYN+WVHQPI+SK+GPRFFGNDF+EMLTRTVWWVIPL
Sbjct: 1   MASKPYTVDLNKPLVFQVGHLGEAYNQWVHQPIVSKEGPRFFGNDFMEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           VWLPVI WL SVSLSRGLTPSD ASCLAGG+FIWTLLEYTLHRFLFHMETRSYW NTLHY
Sbjct: 61  VWLPVICWLASVSLSRGLTPSDVASCLAGGVFIWTLLEYTLHRFLFHMETRSYWGNTLHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           +LHGCHHKHPMDGLRLVFPP AT ILSVPLWIVIRLTSTPAVAP LFGGGLLGYVMYDVT
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPVATAILSVPLWIVIRLTSTPAVAPTLFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237
           HYYLHHGKP+ GLSQN+KRYHLNHHFR QS+GFGITSPLWDRAFGTYPTTK+ QKNK
Sbjct: 181 HYYLHHGKPTSGLSQNMKRYHLNHHFRVQSQGFGITSPLWDRAFGTYPTTKTIQKNK 237

BLAST of CsGy6G036395 vs. ExPASy TrEMBL
Match: A0A0A0KKX5 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525640 PE=3 SV=1)

HSP 1 Score: 502 bits (1293), Expect = 4.38e-180
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT
Sbjct: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237
           HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK
Sbjct: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237

BLAST of CsGy6G036395 vs. ExPASy TrEMBL
Match: A0A5A7U993 (Dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009740 PE=3 SV=1)

HSP 1 Score: 498 bits (1283), Expect = 1.47e-178
Identity = 234/237 (98.73%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M+SKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISK+GPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           +LHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237
           HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK
Sbjct: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237

BLAST of CsGy6G036395 vs. ExPASy TrEMBL
Match: A0A1S3AZN1 (dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Cucumis melo OX=3656 GN=LOC103484300 PE=3 SV=1)

HSP 1 Score: 498 bits (1283), Expect = 1.47e-178
Identity = 234/237 (98.73%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M+SKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISK+GPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           +LHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237
           HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK
Sbjct: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237

BLAST of CsGy6G036395 vs. ExPASy TrEMBL
Match: A0A6J1D0B7 (dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Momordica charantia OX=3673 GN=LOC111015844 PE=3 SV=1)

HSP 1 Score: 470 bits (1209), Expect = 2.82e-167
Identity = 215/237 (90.72%), Postives = 227/237 (95.78%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M+SKP+TVDLNKPLVFQVGHLGEAYN+WVHQPI+SK+GPRFFGNDF+EMLTRTVWWVIPL
Sbjct: 1   MASKPYTVDLNKPLVFQVGHLGEAYNQWVHQPIVSKEGPRFFGNDFMEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           VWLPVI WL SVSLSRGLTPSD ASCLAGG+FIWTLLEYTLHRFLFHMETRSYW NTLHY
Sbjct: 61  VWLPVICWLASVSLSRGLTPSDVASCLAGGVFIWTLLEYTLHRFLFHMETRSYWGNTLHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           +LHGCHHKHPMDGLRLVFPP AT ILSVPLWIVIRLTSTPAVAP LFGGGLLGYVMYDVT
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPVATAILSVPLWIVIRLTSTPAVAPTLFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237
           HYYLHHGKP+ GLSQN+KRYHLNHHFR QS+GFGITSPLWDRAFGTYPTTK+ QKNK
Sbjct: 181 HYYLHHGKPTSGLSQNMKRYHLNHHFRVQSQGFGITSPLWDRAFGTYPTTKTIQKNK 237

BLAST of CsGy6G036395 vs. ExPASy TrEMBL
Match: A0A6J1ECD2 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111433035 PE=3 SV=1)

HSP 1 Score: 468 bits (1203), Expect = 2.32e-166
Identity = 216/237 (91.14%), Postives = 228/237 (96.20%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M+SK FTVDLNKPLVFQVGHLGEAYN+WVHQPI+SK+GPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKTFTVDLNKPLVFQVGHLGEAYNKWVHQPIVSKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           VWLPVISWLVSVSLSRGLTPSD ASCLA G+FIW+LLEYTLHRFLFHMETRSYW NTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDVASCLAAGVFIWSLLEYTLHRFLFHMETRSYWGNTLHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           +LHGCHHKHPMDGLRLVFPPAAT ILSVPLW+VIRLTSTPAVAPALFGGGLLGYVMYDVT
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPAATAILSVPLWVVIRLTSTPAVAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237
           HYYLHHGKP+ GLSQNLKRYHLNHHFR QS+GFGITSPLWDRAFGTYP+TK  Q+NK
Sbjct: 181 HYYLHHGKPTSGLSQNLKRYHLNHHFRIQSQGFGITSPLWDRAFGTYPSTKPIQENK 237

BLAST of CsGy6G036395 vs. TAIR 10
Match: AT2G34770.1 (fatty acid hydroxylase 1 )

HSP 1 Score: 349.7 bits (896), Expect = 1.8e-96
Identity = 152/237 (64.14%), Postives = 186/237 (78.48%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M ++ FTVDL KPLVFQVGHLGE Y EWVHQPI +K+GPRFF +DF E LT TVWW +P+
Sbjct: 1   MVAQGFTVDLKKPLVFQVGHLGEDYEEWVHQPIATKEGPRFFQSDFWEFLTLTVWWAVPV 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           +WLPV+ W +S S+S G +  +    +  GIFIWT  EY LHRF+FH++T+SYW NT HY
Sbjct: 61  IWLPVVVWCISRSVSMGCSLPEIVPIVVMGIFIWTFFEYVLHRFVFHIKTKSYWGNTAHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           ++HGCHHKHPMD LRLVFPP AT IL  P W + +  STP+ APALFGGG+LGYVMYDVT
Sbjct: 121 LIHGCHHKHPMDHLRLVFPPTATAILCFPFWNIAKAISTPSTAPALFGGGMLGYVMYDVT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 238
           HYYLHH +P+  +++NLK+YHLNHHFR Q +GFGITS LWD  FGT PTTK+ +K +
Sbjct: 181 HYYLHHAQPTRPVTKNLKKYHLNHHFRIQDKGFGITSSLWDIVFGTLPTTKAPRKEQ 237

BLAST of CsGy6G036395 vs. TAIR 10
Match: AT4G20870.1 (fatty acid hydroxylase 2 )

HSP 1 Score: 349.7 bits (896), Expect = 1.8e-96
Identity = 153/235 (65.11%), Postives = 186/235 (79.15%), Query Frame = 0

Query: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60
           M ++ +TVDLNKPLVFQVGHLGE Y EW+HQPI+  +GPRFF +DF E LTRTVWW IP 
Sbjct: 1   MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPT 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGIFIWTLLEYTLHRFLFHMETRSYWANTLHY 120
           +WLPV+ +++S+S S+GLT       +A G+  WTLLEYTLHRFLFH++T+SYWANT HY
Sbjct: 61  IWLPVVCYVLSISASKGLTFPQIGLIVAFGVLTWTLLEYTLHRFLFHIQTKSYWANTAHY 120

Query: 121 VLHGCHHKHPMDGLRLVFPPAATTILSVPLWIVIRLTSTPAVAPALFGGGLLGYVMYDVT 180
           +LHGCHHKHP DGLRLVFPP AT IL VPLW ++ L +TPA APA+ GG L GYVMYD+T
Sbjct: 121 LLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATPATAPAILGGILFGYVMYDIT 180

Query: 181 HYYLHHGKPSPGLSQNLKRYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQK 236
           HYYLHHG+P     ++LK+YHLNHHFR Q +G+GITS LWD+ FGT P  K+  K
Sbjct: 181 HYYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGTLPGIKAAAK 235

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O489162.5e-9564.14Dihydroceramide fatty acyl 2-hydroxylase FAH1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SUC52.5e-9565.11Dihydroceramide fatty acyl 2-hydroxylase FAH2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q2LAM06.3e-5445.64Fatty acid 2-hydroxylase OS=Rattus norvegicus OX=10116 GN=Fa2h PE=1 SV=2[more]
Q4R4P41.4e-5345.42Fatty acid 2-hydroxylase OS=Macaca fascicularis OX=9541 GN=FA2H PE=2 SV=1[more]
Q7L5A85.3e-5342.63Fatty acid 2-hydroxylase OS=Homo sapiens OX=9606 GN=FA2H PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004134612.19.05e-180100.00dihydroceramide fatty acyl 2-hydroxylase FAH2 [Cucumis sativus] >KGN49479.1 hypo... [more]
XP_008439533.13.03e-17898.73PREDICTED: dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Cucumis melo] >KA... [more]
XP_038881178.13.01e-16993.25dihydroceramide fatty acyl 2-hydroxylase FAH1-like isoform X1 [Benincasa hispida... [more]
XP_023544587.15.83e-16791.56dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo] ... [more]
XP_022146704.15.83e-16790.72dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Momordica charantia] >XP_022... [more]
Match NameE-valueIdentityDescription
A0A0A0KKX54.38e-180100.00Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A5A7U9931.47e-17898.73Dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Cucumis melo var. makuwa O... [more]
A0A1S3AZN11.47e-17898.73dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1D0B72.82e-16790.72dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Momordica charantia OX=367... [more]
A0A6J1ECD22.32e-16691.14dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT2G34770.11.8e-9664.14fatty acid hydroxylase 1 [more]
AT4G20870.11.8e-9665.11fatty acid hydroxylase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 90..226
e-value: 2.9E-17
score: 63.3
IPR014430Sterol desaturase Scs7PANTHERPTHR12863FATTY ACID HYDROXYLASEcoord: 1..237
NoneNo IPR availablePANTHERPTHR12863:SF12DIHYDROCERAMIDE FATTY ACYL 2-HYDROXYLASE FAH1-LIKEcoord: 1..237

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G036395.1CsGy6G036395.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
biological_process GO:0008610 lipid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0080132 fatty acid alpha-hydroxylase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity