CsGy6G030540 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G030540
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionDNA-3-methyladenine glycosylase 1-like
LocationGy14Chr6: 28668397 .. 28669456 (-)
RNA-Seq ExpressionCsGy6G030540
SyntenyCsGy6G030540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAAAACCTACCTTTTTACCCACAAATCATTCACCGTCGCCCATGGCCAAAAGAATCCGCCGGAAATGCCTCTTTCAGTTGGAGTCTCCATCCGACGCTGTTCCTCTCTCTCCCTCGGCTTCCTCCAAGATTCCCTTCCCATCCACAAAAGTTCGGAAGATTTCCTCCAATCAAGAACCGACCAAACCACAAATTTCAGCTCCCGGCGGTTATAACCCGACCCGAATATTCCCGAACCTCGCCGATCCCGTCAAATCCTTGTCGTCTTCAGATAAAATTTCTACAGCGATCAATCATTTACGCCGTTCGGATCCTCTTCTAATAAGTTTGTTAGATTCTTGCGAAACCCCCAATTTCAAGTCCAATCCACCGTTCTTAGCACTAACGAAGAGCATCCTCTACCAACAACTCGCCACAAAGGCCGCCGAAGCGATCTACAATCGCTTCGCCTCGCTATGCGGCGGAGAGGCGGCGGTACTGCCGGACACCGTGCTCGGACTCTCGCCGCAACAGCTGCGAGTAATCGGAGTTTCGGGGAGGAAGGCAAGTTACCTCCATGATCTAGCAACGAAATTCATAGAGGGGAGTTTATCAAATTCATTTATTCTGGAGATGGACGACGAGACTCTATTGAGGGCGTTAACGGCGGTGAAGGGAATCGGCGTTTGGTCGGTGCATATGTTCATGATATTTACCCTACATCGGCCGGATGTGCTGCCCGTGGGGGATTTGGGAGTGAGAAAAGGGGTGCAGAGATTGTACGGACTGAAGGAATTGCCGAAGCCGGCGGAGATGGAGAAACTTTGTGAGAAATGGAAGCCTTACAGATCGATCGGAGCTTGGTATATGTGGAGGCTAATCGACGCTAAGGAAATCGTGAAGAATGGTTGCGATTGACCGGATAACATGGAATACAGAGGTAGCTTTGCAGTGTGACTTTTGAATTCGTGATTTTCAAATTTGGCTAGTTTTTGTGTCTGTGTAATTTTAAGAACTCAACGTTAAAATGTGTTTGAGTCCTTTGATGAGTGTCTCATTTCATGTTGAATCAATTGAAAC

mRNA sequence

TTCAAAACCTACCTTTTTACCCACAAATCATTCACCGTCGCCCATGGCCAAAAGAATCCGCCGGAAATGCCTCTTTCAGTTGGAGTCTCCATCCGACGCTGTTCCTCTCTCTCCCTCGGCTTCCTCCAAGATTCCCTTCCCATCCACAAAAGTTCGGAAGATTTCCTCCAATCAAGAACCGACCAAACCACAAATTTCAGCTCCCGGCGGTTATAACCCGACCCGAATATTCCCGAACCTCGCCGATCCCGTCAAATCCTTGTCGTCTTCAGATAAAATTTCTACAGCGATCAATCATTTACGCCGTTCGGATCCTCTTCTAATAAGTTTGTTAGATTCTTGCGAAACCCCCAATTTCAAGTCCAATCCACCGTTCTTAGCACTAACGAAGAGCATCCTCTACCAACAACTCGCCACAAAGGCCGCCGAAGCGATCTACAATCGCTTCGCCTCGCTATGCGGCGGAGAGGCGGCGGTACTGCCGGACACCGTGCTCGGACTCTCGCCGCAACAGCTGCGAGTAATCGGAGTTTCGGGGAGGAAGGCAAGTTACCTCCATGATCTAGCAACGAAATTCATAGAGGGGAGTTTATCAAATTCATTTATTCTGGAGATGGACGACGAGACTCTATTGAGGGCGTTAACGGCGGTGAAGGGAATCGGCGTTTGGTCGGTGCATATGTTCATGATATTTACCCTACATCGGCCGGATGTGCTGCCCGTGGGGGATTTGGGAGTGAGAAAAGGGGTGCAGAGATTGTACGGACTGAAGGAATTGCCGAAGCCGGCGGAGATGGAGAAACTTTGTGAGAAATGGAAGCCTTACAGATCGATCGGAGCTTGGTATATGTGGAGGCTAATCGACGCTAAGGAAATCGTGAAGAATGGTTGCGATTGACCGGATAACATGGAATACAGAGGTAGCTTTGCAGTGTGACTTTTGAATTCGTGATTTTCAAATTTGGCTAGTTTTTGTGTCTGTGTAATTTTAAGAACTCAACGTTAAAATGTGTTTGAGTCCTTTGATGAGTGTCTCATTTCATGTTGAATCAATTGAAAC

Coding sequence (CDS)

ATGGCCAAAAGAATCCGCCGGAAATGCCTCTTTCAGTTGGAGTCTCCATCCGACGCTGTTCCTCTCTCTCCCTCGGCTTCCTCCAAGATTCCCTTCCCATCCACAAAAGTTCGGAAGATTTCCTCCAATCAAGAACCGACCAAACCACAAATTTCAGCTCCCGGCGGTTATAACCCGACCCGAATATTCCCGAACCTCGCCGATCCCGTCAAATCCTTGTCGTCTTCAGATAAAATTTCTACAGCGATCAATCATTTACGCCGTTCGGATCCTCTTCTAATAAGTTTGTTAGATTCTTGCGAAACCCCCAATTTCAAGTCCAATCCACCGTTCTTAGCACTAACGAAGAGCATCCTCTACCAACAACTCGCCACAAAGGCCGCCGAAGCGATCTACAATCGCTTCGCCTCGCTATGCGGCGGAGAGGCGGCGGTACTGCCGGACACCGTGCTCGGACTCTCGCCGCAACAGCTGCGAGTAATCGGAGTTTCGGGGAGGAAGGCAAGTTACCTCCATGATCTAGCAACGAAATTCATAGAGGGGAGTTTATCAAATTCATTTATTCTGGAGATGGACGACGAGACTCTATTGAGGGCGTTAACGGCGGTGAAGGGAATCGGCGTTTGGTCGGTGCATATGTTCATGATATTTACCCTACATCGGCCGGATGTGCTGCCCGTGGGGGATTTGGGAGTGAGAAAAGGGGTGCAGAGATTGTACGGACTGAAGGAATTGCCGAAGCCGGCGGAGATGGAGAAACTTTGTGAGAAATGGAAGCCTTACAGATCGATCGGAGCTTGGTATATGTGGAGGCTAATCGACGCTAAGGAAATCGTGAAGAATGGTTGCGATTGA

Protein sequence

MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPTRIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILYQQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIEGSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD*
Homology
BLAST of CsGy6G030540 vs. ExPASy Swiss-Prot
Match: Q92383 (DNA-3-methyladenine glycosylase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mag1 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 4.8e-24
Identity = 58/169 (34.32%), Postives = 93/169 (55.03%), Query Frame = 0

Query: 104 NFKSNPPFLALTKSILYQQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGV 163
           + +   P+  L +++  QQL +KAA AI+NRF S+        P+ +  +  + +R  G 
Sbjct: 44  SMEKKEPYEELIRAVASQQLHSKAANAIFNRFKSISNNGQFPTPEEIRDMDFEIMRACGF 103

Query: 164 SGRKASYLHDLATKFIEGSLSNSFILE-MDDETLLRALTAVKGIGVWSVHMFMIFTLHRP 223
           S RK   L  +A   I G +      E + +E L+  LT +KGIG W+V M +IF+L+R 
Sbjct: 104 SARKIDSLKSIAEATISGLIPTKEEAERLSNEELIERLTQIKGIGRWTVEMLLIFSLNRD 163

Query: 224 DVLPVGDLGVRKGVQRLYGLKELPKPAEMEKLCEKWKPYRSIGAWYMWR 272
           DV+P  DL +R G + L+ L ++P    + K  E   P+R+  AWY+W+
Sbjct: 164 DVMPADDLSIRNGYRYLHRLPKIPTKMYVLKHSEICAPFRTAAAWYLWK 212

BLAST of CsGy6G030540 vs. ExPASy Swiss-Prot
Match: O94468 (Alkylbase DNA glycosidase-like protein mag2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mag2 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.4e-22
Identity = 58/205 (28.29%), Postives = 103/205 (50.24%), Query Frame = 0

Query: 73  LSSSDKISTAINHLRRSD---PLLISLLDSCETPNFKSNPPFLALTKSILYQQLATKAAE 132
           +S       A  HL   D     L+  +  C       + P+  + ++I  Q+L+  A  
Sbjct: 1   MSKDSDYKRAEKHLSSIDNKWSSLVKKVGPCTLTPHPEHAPYEGIIRAITSQKLSDAATN 60

Query: 133 AIYNRFASLCG-GEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIEGSL-SNSF 192
           +I N+F + C   +    P  ++    + L   G S  K+  +H +A   +   + S S 
Sbjct: 61  SIINKFCTQCSDNDEFPTPKQIMETDVETLHECGFSKLKSQEIHIVAEAALNKQIPSKSE 120

Query: 193 ILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPK 252
           I +M +E L+ +L+ +KG+  W++ M+ IFTL R D++P  D  ++   +  +GL   P+
Sbjct: 121 IEKMSEEELMESLSKIKGVKRWTIEMYSIFTLGRLDIMPADDSTLKNEAKEFFGLSSKPQ 180

Query: 253 PAEMEKLCEKWKPYRSIGAWYMWRL 273
             E+EKL +  KPYR+I AWY+W++
Sbjct: 181 TEEVEKLTKPCKPYRTIAAWYLWQI 205

BLAST of CsGy6G030540 vs. ExPASy Swiss-Prot
Match: O31544 (Putative DNA-3-methyladenine glycosylase YfjP OS=Bacillus subtilis (strain 168) OX=224308 GN=yfjP PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.8e-21
Identity = 56/178 (31.46%), Postives = 94/178 (52.81%), Query Frame = 0

Query: 102 TPNFKSNPPFLALTKSILYQQLATKAAEAIYNRFASLCGGEAAVL-----PDTVLGLSPQ 161
           TP       +  + K I++QQL    A  +  RF    G +   +     P+T+  L  Q
Sbjct: 110 TPLVLDYSVYNCMMKCIIHQQLNLSFAYTLTERFVHAFGEQKDGVWCYPKPETIAELDYQ 169

Query: 162 QLRVIGVSGRKASYLHDLATKFIEGSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMI 221
            LR +  S RKA Y  D +    EG+LS S +  M DE +++ L  ++GIG W+V   ++
Sbjct: 170 DLRDLQFSMRKAEYTIDTSRMIAEGTLSLSELPHMADEDIMKKLIKIRGIGPWTVQNVLM 229

Query: 222 FTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLID 275
           F L RP++ P+ D+G++  ++R + L + P    M  + ++W+PY S  + Y+WR I+
Sbjct: 230 FGLGRPNLFPLADIGLQNAIKRHFQLDDKPAKDVMLAMSKEWEPYLSYASLYLWRSIE 287

BLAST of CsGy6G030540 vs. ExPASy Swiss-Prot
Match: P22134 (DNA-3-methyladenine glycosylase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MAG1 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.1e-15
Identity = 73/238 (30.67%), Postives = 105/238 (44.12%), Query Frame = 0

Query: 77  DKISTAINHLRRSDPLLISLLDSCE---------TPNFKSNPPFLALTKSILYQQLATKA 136
           +K + A  H+   DP L  +L + E          PN   +  F+ L  +IL QQ++ +A
Sbjct: 42  EKFNMACEHILEKDPSLFPILKNNEFTLYLKETQVPNTLED-YFIRLASTILSQQISGQA 101

Query: 137 AEAIYNRFASLCGGE----AAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE--G 196
           AE+I  R  SL GG       +  D        ++   G+S RK  YL  LA  F E   
Sbjct: 102 AESIKARVVSLYGGAFPDYKILFEDFKDPAKCAEIAKCGLSKRKMIYLESLAVYFTEKYK 161

Query: 197 SLSNSFILEMDDETLLRAL-TAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 256
            +   F  + +DE ++ +L T VKGIG WS  MF+I  L R DV    DLG+ +G  +  
Sbjct: 162 DIEKLFGQKDNDEEVIESLVTNVKGIGPWSAKMFLISGLKRMDVFAPEDLGIARGFSKYL 221

Query: 257 G-----LKELPKPAE---------------------MEKLCEKWKPYRSIGAWYMWRL 273
                  KEL +  +                     MEK  E + PYRS+  + +WRL
Sbjct: 222 SDKPELEKELMRERKVVKKSKIKHKKYNWKIYDDDIMEKCSETFSPYRSVFMFILWRL 278

BLAST of CsGy6G030540 vs. NCBI nr
Match: XP_004143510.1 (DNA-3-methyladenine glycosylase 1 [Cucumis sativus] >KGN48831.1 hypothetical protein Csa_003752 [Cucumis sativus])

HSP 1 Score: 569 bits (1466), Expect = 1.45e-204
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60
           MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT
Sbjct: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60

Query: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120
           RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY
Sbjct: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120

Query: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180
           QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE
Sbjct: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180

Query: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240
           GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240

Query: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284
           GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD
Sbjct: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284

BLAST of CsGy6G030540 vs. NCBI nr
Match: XP_008440714.1 (PREDICTED: probable DNA-3-methyladenine glycosylase 2 [Cucumis melo] >KAA0036227.1 putative DNA-3-methyladenine glycosylase 2 [Cucumis melo var. makuwa] >TYK12621.1 putative DNA-3-methyladenine glycosylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 514 bits (1323), Expect = 1.27e-182
Identity = 256/284 (90.14%), Postives = 268/284 (94.37%), Query Frame = 0

Query: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60
           MAKRIRRK LFQ ESP+ AVPLSPS+SSKIPF STKVRKISSNQEP KPQ SAP GYNPT
Sbjct: 1   MAKRIRRKFLFQSESPTGAVPLSPSSSSKIPFRSTKVRKISSNQEPAKPQFSAPDGYNPT 60

Query: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120
           R FPNLADPVKSLSS D+ISTAINHLRRSDPLLISLLDSCE+P+FKSNPPFLALTKSILY
Sbjct: 61  RTFPNLADPVKSLSSLDEISTAINHLRRSDPLLISLLDSCESPHFKSNPPFLALTKSILY 120

Query: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180
           QQLATKAAE+IYNRFASLCGGEA+VLPDTVLGLSPQQLRV+GVSGRKASYLHDLATKFIE
Sbjct: 121 QQLATKAAESIYNRFASLCGGEASVLPDTVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 180

Query: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240
           G+LSNS ILEMDDETLL  LTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 181 GNLSNSLILEMDDETLLGELTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240

Query: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284
           GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRL++ K +VK G D
Sbjct: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLMEEKGVVKKGSD 284

BLAST of CsGy6G030540 vs. NCBI nr
Match: XP_038881017.1 (DNA-3-methyladenine glycosylase 1-like [Benincasa hispida])

HSP 1 Score: 478 bits (1229), Expect = 1.48e-168
Identity = 239/276 (86.59%), Postives = 255/276 (92.39%), Query Frame = 0

Query: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60
           MAKRIRRK L Q +S  DA PL PS+SSKIPFPSTKVRKISS QEP KPQIS  GG +PT
Sbjct: 1   MAKRIRRKFLVQSDSRIDADPLPPSSSSKIPFPSTKVRKISSKQEPAKPQISTSGGNDPT 60

Query: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120
           R F NLA P+KSLSSSD+I TAI+HLRRSDPLLIS+L+SCE+PNFKSNPPFLALTKSILY
Sbjct: 61  RAFQNLASPLKSLSSSDEIFTAIDHLRRSDPLLISILESCESPNFKSNPPFLALTKSILY 120

Query: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180
           QQLATKAAE+IYNRFASLCGGE +VLPD VLGLSPQQLRVIGVSGRKASYLHDLATKFIE
Sbjct: 121 QQLATKAAESIYNRFASLCGGEVSVLPDIVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180

Query: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240
           G+LSNS ILEMDDETLL ALTAVKGIG+WSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 181 GNLSNSMILEMDDETLLEALTAVKGIGMWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240

Query: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAK 276
           GLKELPKPAEMEKLCEKWKPYRS+GAWYMWRL++ K
Sbjct: 241 GLKELPKPAEMEKLCEKWKPYRSMGAWYMWRLMEVK 276

BLAST of CsGy6G030540 vs. NCBI nr
Match: XP_023543059.1 (DNA-3-methyladenine glycosylase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 461 bits (1185), Expect = 4.36e-161
Identity = 235/284 (82.75%), Postives = 250/284 (88.03%), Query Frame = 0

Query: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60
           MA+R RRK L Q ES ++A P S     KI F +TK+RKISS Q+P KPQIS PGG + T
Sbjct: 35  MARRTRRKLLLQSESQTEADPPS-----KISFRTTKIRKISSTQKPDKPQISTPGGGDRT 94

Query: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120
           R FPN   PVKSLSSSD I TAI+HLRRSDPLLI LLDSCE+PNFKSNPPFLALTKSILY
Sbjct: 95  RAFPNQDGPVKSLSSSDVIRTAIDHLRRSDPLLIRLLDSCESPNFKSNPPFLALTKSILY 154

Query: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180
           QQLATKAAE+IYNRFASLCGGEAAVLPD VLGLSPQQLRV+GVSGRKASYLHDLATKFIE
Sbjct: 155 QQLATKAAESIYNRFASLCGGEAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 214

Query: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240
           GSLSNS ILEMDDETLL ALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 215 GSLSNSSILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 274

Query: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284
           GLKELPKP EMEKLCE WKPYRS+GAWYMWRL++ KEIVK+  D
Sbjct: 275 GLKELPKPVEMEKLCENWKPYRSMGAWYMWRLMEMKEIVKDDGD 313

BLAST of CsGy6G030540 vs. NCBI nr
Match: XP_022949777.1 (DNA-3-methyladenine glycosylase 1-like [Cucurbita moschata])

HSP 1 Score: 458 bits (1178), Expect = 5.07e-160
Identity = 234/284 (82.39%), Postives = 249/284 (87.68%), Query Frame = 0

Query: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60
           MA+R RRK L Q ES +DA P S      I F +TK+RKISS Q+  KPQIS PGG + T
Sbjct: 35  MARRTRRKLLLQSESQTDADPPS-----NISFRTTKIRKISSTQKSDKPQISTPGGGDRT 94

Query: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120
           R FPN   PVKSLSSSD I TAI+HLRRSDPLLI LLDSCE+PNFKSNPPFLALTKSILY
Sbjct: 95  RAFPNQDGPVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKSNPPFLALTKSILY 154

Query: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180
           QQLATKAAE+IYNRFASLCGG+AAVLPD VLGLSPQQLRV+GVSGRKASYLHDLATKFIE
Sbjct: 155 QQLATKAAESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 214

Query: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240
           GSLSNS ILEMDDETLL ALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 215 GSLSNSSILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 274

Query: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284
           GLKELPKP EMEKLCEKWKPYRS+GAWYMWRL++ KEIVK+  D
Sbjct: 275 GLKELPKPVEMEKLCEKWKPYRSMGAWYMWRLMEMKEIVKDDGD 313

BLAST of CsGy6G030540 vs. ExPASy TrEMBL
Match: A0A0A0KM62 (ENDO3c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G502690 PE=4 SV=1)

HSP 1 Score: 569 bits (1466), Expect = 7.03e-205
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60
           MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT
Sbjct: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60

Query: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120
           RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY
Sbjct: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120

Query: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180
           QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE
Sbjct: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180

Query: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240
           GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240

Query: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284
           GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD
Sbjct: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284

BLAST of CsGy6G030540 vs. ExPASy TrEMBL
Match: A0A5A7T3R0 (Putative DNA-3-methyladenine glycosylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002080 PE=4 SV=1)

HSP 1 Score: 514 bits (1323), Expect = 6.13e-183
Identity = 256/284 (90.14%), Postives = 268/284 (94.37%), Query Frame = 0

Query: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60
           MAKRIRRK LFQ ESP+ AVPLSPS+SSKIPF STKVRKISSNQEP KPQ SAP GYNPT
Sbjct: 1   MAKRIRRKFLFQSESPTGAVPLSPSSSSKIPFRSTKVRKISSNQEPAKPQFSAPDGYNPT 60

Query: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120
           R FPNLADPVKSLSS D+ISTAINHLRRSDPLLISLLDSCE+P+FKSNPPFLALTKSILY
Sbjct: 61  RTFPNLADPVKSLSSLDEISTAINHLRRSDPLLISLLDSCESPHFKSNPPFLALTKSILY 120

Query: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180
           QQLATKAAE+IYNRFASLCGGEA+VLPDTVLGLSPQQLRV+GVSGRKASYLHDLATKFIE
Sbjct: 121 QQLATKAAESIYNRFASLCGGEASVLPDTVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 180

Query: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240
           G+LSNS ILEMDDETLL  LTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 181 GNLSNSLILEMDDETLLGELTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240

Query: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284
           GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRL++ K +VK G D
Sbjct: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLMEEKGVVKKGSD 284

BLAST of CsGy6G030540 vs. ExPASy TrEMBL
Match: A0A1S3B2D5 (probable DNA-3-methyladenine glycosylase 2 OS=Cucumis melo OX=3656 GN=LOC103485049 PE=4 SV=1)

HSP 1 Score: 514 bits (1323), Expect = 6.13e-183
Identity = 256/284 (90.14%), Postives = 268/284 (94.37%), Query Frame = 0

Query: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60
           MAKRIRRK LFQ ESP+ AVPLSPS+SSKIPF STKVRKISSNQEP KPQ SAP GYNPT
Sbjct: 1   MAKRIRRKFLFQSESPTGAVPLSPSSSSKIPFRSTKVRKISSNQEPAKPQFSAPDGYNPT 60

Query: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120
           R FPNLADPVKSLSS D+ISTAINHLRRSDPLLISLLDSCE+P+FKSNPPFLALTKSILY
Sbjct: 61  RTFPNLADPVKSLSSLDEISTAINHLRRSDPLLISLLDSCESPHFKSNPPFLALTKSILY 120

Query: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180
           QQLATKAAE+IYNRFASLCGGEA+VLPDTVLGLSPQQLRV+GVSGRKASYLHDLATKFIE
Sbjct: 121 QQLATKAAESIYNRFASLCGGEASVLPDTVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 180

Query: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240
           G+LSNS ILEMDDETLL  LTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 181 GNLSNSLILEMDDETLLGELTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240

Query: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284
           GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRL++ K +VK G D
Sbjct: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLMEEKGVVKKGSD 284

BLAST of CsGy6G030540 vs. ExPASy TrEMBL
Match: A0A6J1GD23 (DNA-3-methyladenine glycosylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453068 PE=4 SV=1)

HSP 1 Score: 458 bits (1178), Expect = 2.45e-160
Identity = 234/284 (82.39%), Postives = 249/284 (87.68%), Query Frame = 0

Query: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60
           MA+R RRK L Q ES +DA P S      I F +TK+RKISS Q+  KPQIS PGG + T
Sbjct: 35  MARRTRRKLLLQSESQTDADPPS-----NISFRTTKIRKISSTQKSDKPQISTPGGGDRT 94

Query: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120
           R FPN   PVKSLSSSD I TAI+HLRRSDPLLI LLDSCE+PNFKSNPPFLALTKSILY
Sbjct: 95  RAFPNQDGPVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKSNPPFLALTKSILY 154

Query: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180
           QQLATKAAE+IYNRFASLCGG+AAVLPD VLGLSPQQLRV+GVSGRKASYLHDLATKFIE
Sbjct: 155 QQLATKAAESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 214

Query: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240
           GSLSNS ILEMDDETLL ALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 215 GSLSNSSILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 274

Query: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284
           GLKELPKP EMEKLCEKWKPYRS+GAWYMWRL++ KEIVK+  D
Sbjct: 275 GLKELPKPVEMEKLCEKWKPYRSMGAWYMWRLMEMKEIVKDDGD 313

BLAST of CsGy6G030540 vs. ExPASy TrEMBL
Match: A0A6J1IQD1 (DNA-3-methyladenine glycosylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478481 PE=4 SV=1)

HSP 1 Score: 450 bits (1158), Expect = 2.92e-157
Identity = 228/284 (80.28%), Postives = 247/284 (86.97%), Query Frame = 0

Query: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60
           MA+R RRK L Q ES ++A P S     KI F +T++RKISS ++P KPQIS  GG + T
Sbjct: 35  MARRTRRKLLLQSESQTEADPPS-----KISFRTTEIRKISSTRKPDKPQISTDGGGDRT 94

Query: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120
           R FPN   PVKSLSSSD I TAI+HLRRSDPLLI LLDSCE+PNFKSNPPFLA+TKSILY
Sbjct: 95  RAFPNQDGPVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKSNPPFLAITKSILY 154

Query: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180
           QQLATKAAE+IYNRFASLCGGEAAVLPD VLGLSPQQLRV+GVSGRKASYLHDLATKF+E
Sbjct: 155 QQLATKAAESIYNRFASLCGGEAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFVE 214

Query: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240
           G+LSNS ILEMDDETLL ALT VKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 215 GTLSNSSILEMDDETLLSALTGVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 274

Query: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284
           GLKELPKP EMEKLCEKWKPYRS+GAWYMWRL++ K I KN  D
Sbjct: 275 GLKELPKPVEMEKLCEKWKPYRSMGAWYMWRLMEMKGIAKNDGD 313

BLAST of CsGy6G030540 vs. TAIR 10
Match: AT1G19480.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 276.6 bits (706), Expect = 2.3e-74
Identity = 151/286 (52.80%), Postives = 192/286 (67.13%), Query Frame = 0

Query: 2   AKRIRRKCLFQLESPSDAVPLSPSASSKIPFPST------KVRKISSNQ--EPTKPQISA 61
           A+RI    L  + SP   +PL P    K+           K   ISS+Q   P      +
Sbjct: 54  ARRITE--LGNVSSPPSKIPLRPRKIRKLTLDGDVSGEDYKAEDISSSQVNSPLATDGKS 113

Query: 62  PGGYNPTRIFPNLADPVKS--LSSSDKISTAINHLRRSDPLLISLLDSCETPNFKS-NPP 121
           PG    + +       +++  L+   ++ TAI++LR +DPLL +L+D    P F+S   P
Sbjct: 114 PGKGKLSHLRAITVPRIQARPLTCEGELETAIHYLRNADPLLAALIDVHPPPTFESFKTP 173

Query: 122 FLALTKSILYQQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASY 181
           FLAL ++ILYQQLA KA  +IY RF SLCGGE  V+P+TVL L+PQQLR IGVSGRKASY
Sbjct: 174 FLALIRNILYQQLAMKAGNSIYTRFVSLCGGENLVVPETVLSLNPQQLRQIGVSGRKASY 233

Query: 182 LHDLATKFIEGSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDL 241
           LHDLA K+  G LS+S IL MD+++L   LT V GIG WSVHMFMI +LHRPDVLPV DL
Sbjct: 234 LHDLARKYQNGILSDSAILNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDL 293

Query: 242 GVRKGVQRLYGLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAK 277
           GVRKGVQ LYGL +LP+P++ME+ C KW+PYRS+G+WYMWRLI+AK
Sbjct: 294 GVRKGVQLLYGLDDLPRPSQMEQHCAKWRPYRSVGSWYMWRLIEAK 337

BLAST of CsGy6G030540 vs. TAIR 10
Match: AT1G19480.2 (DNA glycosylase superfamily protein )

HSP 1 Score: 276.6 bits (706), Expect = 2.3e-74
Identity = 151/286 (52.80%), Postives = 192/286 (67.13%), Query Frame = 0

Query: 2   AKRIRRKCLFQLESPSDAVPLSPSASSKIPFPST------KVRKISSNQ--EPTKPQISA 61
           A+RI    L  + SP   +PL P    K+           K   ISS+Q   P      +
Sbjct: 54  ARRITE--LGNVSSPPSKIPLRPRKIRKLTLDGDVSGEDYKAEDISSSQVNSPLATDGKS 113

Query: 62  PGGYNPTRIFPNLADPVKS--LSSSDKISTAINHLRRSDPLLISLLDSCETPNFKS-NPP 121
           PG    + +       +++  L+   ++ TAI++LR +DPLL +L+D    P F+S   P
Sbjct: 114 PGKGKLSHLRAITVPRIQARPLTCEGELETAIHYLRNADPLLAALIDVHPPPTFESFKTP 173

Query: 122 FLALTKSILYQQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASY 181
           FLAL ++ILYQQLA KA  +IY RF SLCGGE  V+P+TVL L+PQQLR IGVSGRKASY
Sbjct: 174 FLALIRNILYQQLAMKAGNSIYTRFVSLCGGENLVVPETVLSLNPQQLRQIGVSGRKASY 233

Query: 182 LHDLATKFIEGSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDL 241
           LHDLA K+  G LS+S IL MD+++L   LT V GIG WSVHMFMI +LHRPDVLPV DL
Sbjct: 234 LHDLARKYQNGILSDSAILNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDL 293

Query: 242 GVRKGVQRLYGLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAK 277
           GVRKGVQ LYGL +LP+P++ME+ C KW+PYRS+G+WYMWRLI+AK
Sbjct: 294 GVRKGVQLLYGLDDLPRPSQMEQHCAKWRPYRSVGSWYMWRLIEAK 337

BLAST of CsGy6G030540 vs. TAIR 10
Match: AT1G75230.2 (DNA glycosylase superfamily protein )

HSP 1 Score: 274.6 bits (701), Expect = 8.8e-74
Identity = 143/273 (52.38%), Postives = 184/273 (67.40%), Query Frame = 0

Query: 25  SASSKIPFPSTKVRKIS---------------SNQEPTKPQISAPGGYNPTRIFPNLADP 84
           S  +KIP    K+RK+S               S    TKP   +    + T   P +   
Sbjct: 68  SPPTKIPLRPRKIRKLSPDDDASDGFNPEHNLSQMTTTKPATKSKLSQSRTVTVPRI--Q 127

Query: 85  VKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKS-NPPFLALTKSILYQQLATKAA 144
            +SL+   ++  A++HLR  DPLL SL+D    P F++   PFLAL +SILYQQLA KA 
Sbjct: 128 ARSLTCEGELEAALHHLRSVDPLLASLIDIHPPPTFETFQTPFLALIRSILYQQLAAKAG 187

Query: 145 EAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIEGSLSNSFI 204
            +IY RF +LCGGE  V+P+ VL L+PQQLR IGVSGRKASYLHDLA K+  G LS+S I
Sbjct: 188 NSIYTRFVALCGGENGVVPENVLPLTPQQLRQIGVSGRKASYLHDLARKYQNGILSDSGI 247

Query: 205 LEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKP 264
           + MD+++L   LT V GIG WSVHMFMI +LHRPDVLPV DLGVRKGVQ L G+++LP+P
Sbjct: 248 VNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQMLNGMEDLPRP 307

Query: 265 AEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKN 282
           ++ME+LCEKW+PYRS+ +WY+WRLI++K    N
Sbjct: 308 SKMEQLCEKWRPYRSVASWYLWRLIESKNTPPN 338

BLAST of CsGy6G030540 vs. TAIR 10
Match: AT1G75230.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 274.6 bits (701), Expect = 8.8e-74
Identity = 143/273 (52.38%), Postives = 184/273 (67.40%), Query Frame = 0

Query: 25  SASSKIPFPSTKVRKIS---------------SNQEPTKPQISAPGGYNPTRIFPNLADP 84
           S  +KIP    K+RK+S               S    TKP   +    + T   P +   
Sbjct: 68  SPPTKIPLRPRKIRKLSPDDDASDGFNPEHNLSQMTTTKPATKSKLSQSRTVTVPRI--Q 127

Query: 85  VKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKS-NPPFLALTKSILYQQLATKAA 144
            +SL+   ++  A++HLR  DPLL SL+D    P F++   PFLAL +SILYQQLA KA 
Sbjct: 128 ARSLTCEGELEAALHHLRSVDPLLASLIDIHPPPTFETFQTPFLALIRSILYQQLAAKAG 187

Query: 145 EAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIEGSLSNSFI 204
            +IY RF +LCGGE  V+P+ VL L+PQQLR IGVSGRKASYLHDLA K+  G LS+S I
Sbjct: 188 NSIYTRFVALCGGENGVVPENVLPLTPQQLRQIGVSGRKASYLHDLARKYQNGILSDSGI 247

Query: 205 LEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKP 264
           + MD+++L   LT V GIG WSVHMFMI +LHRPDVLPV DLGVRKGVQ L G+++LP+P
Sbjct: 248 VNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQMLNGMEDLPRP 307

Query: 265 AEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKN 282
           ++ME+LCEKW+PYRS+ +WY+WRLI++K    N
Sbjct: 308 SKMEQLCEKWRPYRSVASWYLWRLIESKNTPPN 338

BLAST of CsGy6G030540 vs. TAIR 10
Match: AT3G50880.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 273.9 bits (699), Expect = 1.5e-73
Identity = 145/255 (56.86%), Postives = 182/255 (71.37%), Query Frame = 0

Query: 26  ASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPTRIFPNLADPVKSLSSSDKISTAINH 85
           +SS+I F   K+RK+SS+  P              RI    + P   LS+   +  A+ H
Sbjct: 34  SSSRIRFRPRKIRKVSSDPSP--------------RIIITASPP---LSTKSTVDIALRH 93

Query: 86  LRRSDPLLISLLDSCETPNF--KSNPPFLALTKSILYQQLATKAAEAIYNRFASLC-GGE 145
           L+ SD LL +L+ +   P     SN PFL+L +SILYQQLATKAA+ IY+RF SL  GGE
Sbjct: 94  LQSSDELLGALITTHNDPPLFDSSNTPFLSLARSILYQQLATKAAKCIYDRFISLFNGGE 153

Query: 146 AAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIEGSLSNSFILEMDDETLLRALTA 205
           A V+P++V+ LS   LR IGVSGRKASYLHDLA K+  G LS+  IL+M DE L+  LT 
Sbjct: 154 AGVVPESVISLSAVDLRKIGVSGRKASYLHDLADKYNNGVLSDELILKMSDEELIDRLTL 213

Query: 206 VKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPAEMEKLCEKWKPYR 265
           VKGIGVW+VHMFMIF+LHRPDVLPVGDLGVRKGV+ LYGLK LP P +ME+LCEKW+PYR
Sbjct: 214 VKGIGVWTVHMFMIFSLHRPDVLPVGDLGVRKGVKDLYGLKNLPGPLQMEQLCEKWRPYR 271

Query: 266 SIGAWYMWRLIDAKE 278
           S+G+WYMWRLI++++
Sbjct: 274 SVGSWYMWRLIESRK 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q923834.8e-2434.32DNA-3-methyladenine glycosylase 1 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
O944683.4e-2228.29Alkylbase DNA glycosidase-like protein mag2 OS=Schizosaccharomyces pombe (strain... [more]
O315443.8e-2131.46Putative DNA-3-methyladenine glycosylase YfjP OS=Bacillus subtilis (strain 168) ... [more]
P221343.1e-1530.67DNA-3-methyladenine glycosylase OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
Match NameE-valueIdentityDescription
XP_004143510.11.45e-204100.00DNA-3-methyladenine glycosylase 1 [Cucumis sativus] >KGN48831.1 hypothetical pro... [more]
XP_008440714.11.27e-18290.14PREDICTED: probable DNA-3-methyladenine glycosylase 2 [Cucumis melo] >KAA0036227... [more]
XP_038881017.11.48e-16886.59DNA-3-methyladenine glycosylase 1-like [Benincasa hispida][more]
XP_023543059.14.36e-16182.75DNA-3-methyladenine glycosylase 1 [Cucurbita pepo subsp. pepo][more]
XP_022949777.15.07e-16082.39DNA-3-methyladenine glycosylase 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KM627.03e-205100.00ENDO3c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G502690 PE=4... [more]
A0A5A7T3R06.13e-18390.14Putative DNA-3-methyladenine glycosylase 2 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B2D56.13e-18390.14probable DNA-3-methyladenine glycosylase 2 OS=Cucumis melo OX=3656 GN=LOC1034850... [more]
A0A6J1GD232.45e-16082.39DNA-3-methyladenine glycosylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1IQD12.92e-15780.28DNA-3-methyladenine glycosylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114784... [more]
Match NameE-valueIdentityDescription
AT1G19480.12.3e-7452.80DNA glycosylase superfamily protein [more]
AT1G19480.22.3e-7452.80DNA glycosylase superfamily protein [more]
AT1G75230.28.8e-7452.38DNA glycosylase superfamily protein [more]
AT1G75230.18.8e-7452.38DNA glycosylase superfamily protein [more]
AT3G50880.11.5e-7356.86DNA glycosylase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003265HhH-GPD domainSMARTSM00478endo3endcoord: 119..274
e-value: 6.8E-17
score: 72.1
IPR003265HhH-GPD domainPFAMPF00730HhH-GPDcoord: 116..259
e-value: 7.7E-22
score: 77.8
IPR003265HhH-GPD domainCDDcd00056ENDO3ccoord: 111..272
e-value: 2.8314E-35
score: 122.735
NoneNo IPR availableGENE3D1.10.1670.40coord: 85..271
e-value: 1.6E-68
score: 231.9
NoneNo IPR availableGENE3D1.10.340.30Hypothetical protein; domain 2coord: 110..221
e-value: 1.6E-68
score: 231.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..58
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..54
NoneNo IPR availablePANTHERPTHR43003DNA-3-METHYLADENINE GLYCOSYLASEcoord: 3..281
NoneNo IPR availablePANTHERPTHR43003:SF8HHH-GPD BASE EXCISION DNA REPAIR FAMILY PROTEINcoord: 3..281
IPR011257DNA glycosylaseSUPERFAMILY48150DNA-glycosylasecoord: 108..273

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G030540.1CsGy6G030540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006285 base-excision repair, AP site formation
biological_process GO:0006307 DNA dealkylation involved in DNA repair
biological_process GO:0006284 base-excision repair
biological_process GO:0006281 DNA repair
cellular_component GO:0005634 nucleus
cellular_component GO:0032993 protein-DNA complex
molecular_function GO:0032131 alkylated DNA binding
molecular_function GO:0008725 DNA-3-methyladenine glycosylase activity
molecular_function GO:0043916 DNA-7-methylguanine glycosylase activity
molecular_function GO:0003824 catalytic activity