CsGy5G026240 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G026240
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4-like
LocationGy14Chr5: 30230782 .. 30232563 (-)
RNA-Seq ExpressionCsGy5G026240
SyntenyCsGy5G026240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCACCGTGGGTGGTTCTATTAATTGGTGCAGTTATGAACTTTTTTGGTTACTTTATGATTTGGCTCGGTGTTACTCGCCGAATCTCCACCCCAAAAGTTTGGCAAATGTGTTTCTATATTTGTATGGGAGGAAGTTCCCAATCCTTTGCTAATACCGGTTCTATGGTTACTTGTGTTAATAACTTTCCCGAAAGGCGTGGCGTTGTTCTCGGTAAGTCTTCATTAAAAAAATTTGTATATATACTATGGTTATTTAAAATTTCAATATTAAATGTTGGAGTTTATTAGACATTTTATAAAATTTCAACCTTTCAATTTAGGAAATACTTTCATATAATATTATTGGATTTTGATGCTCAATCACTAATTCATTTCCCAATTTCAAACTTGGATTTAGAATAGGATTATATCTTACTTTAATAATAATAATAAAATCATTATGGCTAATTAGATAACAATAAAATCGTTAATGGCTAATTCAAACGTTGAAAAAAATTAGGTCTTTTGAAGGGATACATCGGGCTAAGCGGCGCCATAATCACACAGCTCTTCCACGCATTCTACGGCGGCGACACTAAATCCCTCATTCTCTTCATCGGATGGCTTCCGGCTGCAATTTCCTTCGCATTCTTACGAACAGTTCGTATCATGAAAGTCATCCGACAGCCAAATGAGCTCAAAGTTTTCTATAATTTCCTCTACATTTCTCTTCTCCTCGCCGGATTCCTCATGCTAATGATCATTGTACAAAGCAAGACAGAGTTCACTCAAAACCAATATGGTGGCAGCGCGGCTGCCATAGTCGTTCTTCTCCTCCTACCACTCGCCGTTGTCACTATCGAAGAATGCAATCTCCAGAAACTTAAAACCAAATCTCCAAATTCTTCTGTTCAAATCATAACTGAGAAACTCCCAAAAACAGAGCATTCAAAACAAAAAGAACCATCGTGTTGGACAACAATTTTCAACCCACCACAACGAGGCGAGGATTTCACAATTCTCCAAGCTGTCTTCAGTGTCGACATGCTGATTTTATTCATATCTGTAATTTGTGGCGCTGGTGGAACGTTAACGGCGGTCGACAATTTGGGTCAAATCGGAATGTCTTTGGGATACCCAAAAAGAAGCATTAGCACATTTGTATCATTAGTCAGTATTTGGAATTACCTCGGTCGAGTTGTCTCTGGTTTCGTCTCTGAAATCGTTTTGATAAAATACAAATTCCCTCGTCCTTTAATGCTTTCTCTGAACCTTCTTTTATCCTGTGTCGGCTACTTGGTCATCGCATTTGACGTCCCAAATGGATTATATGTTGCTTCCATCGTAATCGGATTCTGTGTTGGAGCACAGTGGCCGTTGATTTACGCCATAATTTCAGAGATTTTTGGGTTGAAATATTACTCAACGCTTTACAATTTCGGGACGGTTGCGATGCCAATTGGACTTTATATTATGAATGTGAAAGTGGCTGGGAACTTCTACGACAGAGAAGCAGAGAAACAGCTAAAAGCAAAAGGGATTATACGAAAAGCAGGGGAAGACTTAAAGTGCTATGGAGGGGAATGCTTTAAGCTTTCGTTCATCGTAATCACTGCAGTTACTTTGATGGGTATGTTTATTTCATTGATTTTAGTGATTAGAACAAGAAGTTTTTACAAAAGTGATATTTATAAAAAGTTTAGAGATGAAGCCGAAACAGAGGTGGCCGGAAATGAGGCTGTGGCGACGGCGGGAGGAGCAGAAGAGAGAGAGAAAATTATAATGCAAAGAAGAAGTTGA

mRNA sequence

ATGCCACCGTGGGTGGTTCTATTAATTGGTGCAGTTATGAACTTTTTTGGTTACTTTATGATTTGGCTCGGTGTTACTCGCCGAATCTCCACCCCAAAAGTTTGGCAAATGTGTTTCTATATTTGTATGGGAGGAAGTTCCCAATCCTTTGCTAATACCGGTTCTATGGTTACTTGTGTTAATAACTTTCCCGAAAGGCGTGGCGTTGTTCTCGGTCTTTTGAAGGGATACATCGGGCTAAGCGGCGCCATAATCACACAGCTCTTCCACGCATTCTACGGCGGCGACACTAAATCCCTCATTCTCTTCATCGGATGGCTTCCGGCTGCAATTTCCTTCGCATTCTTACGAACAGTTCGTATCATGAAAGTCATCCGACAGCCAAATGAGCTCAAAGTTTTCTATAATTTCCTCTACATTTCTCTTCTCCTCGCCGGATTCCTCATGCTAATGATCATTGTACAAAGCAAGACAGAGTTCACTCAAAACCAATATGGTGGCAGCGCGGCTGCCATAGTCGTTCTTCTCCTCCTACCACTCGCCGTTGTCACTATCGAAGAATGCAATCTCCAGAAACTTAAAACCAAATCTCCAAATTCTTCTGTTCAAATCATAACTGAGAAACTCCCAAAAACAGAGCATTCAAAACAAAAAGAACCATCGTGTTGGACAACAATTTTCAACCCACCACAACGAGGCGAGGATTTCACAATTCTCCAAGCTGTCTTCAGTGTCGACATGCTGATTTTATTCATATCTGTAATTTGTGGCGCTGGTGGAACGTTAACGGCGGTCGACAATTTGGGTCAAATCGGAATGTCTTTGGGATACCCAAAAAGAAGCATTAGCACATTTGTATCATTAGTCAGTATTTGGAATTACCTCGGTCGAGTTGTCTCTGGTTTCGTCTCTGAAATCGTTTTGATAAAATACAAATTCCCTCGTCCTTTAATGCTTTCTCTGAACCTTCTTTTATCCTGTGTCGGCTACTTGGTCATCGCATTTGACGTCCCAAATGGATTATATGTTGCTTCCATCGTAATCGGATTCTGTGTTGGAGCACAGTGGCCGTTGATTTACGCCATAATTTCAGAGATTTTTGGGTTGAAATATTACTCAACGCTTTACAATTTCGGGACGGTTGCGATGCCAATTGGACTTTATATTATGAATGTGAAAGTGGCTGGGAACTTCTACGACAGAGAAGCAGAGAAACAGCTAAAAGCAAAAGGGATTATACGAAAAGCAGGGGAAGACTTAAAGTGCTATGGAGGGGAATGCTTTAAGCTTTCGTTCATCGTAATCACTGCAGTTACTTTGATGGGTATGTTTATTTCATTGATTTTAGTGATTAGAACAAGAAGTTTTTACAAAAGTGATATTTATAAAAAGTTTAGAGATGAAGCCGAAACAGAGGTGGCCGGAAATGAGGCTGTGGCGACGGCGGGAGGAGCAGAAGAGAGAGAGAAAATTATAATGCAAAGAAGAAGTTGA

Coding sequence (CDS)

ATGCCACCGTGGGTGGTTCTATTAATTGGTGCAGTTATGAACTTTTTTGGTTACTTTATGATTTGGCTCGGTGTTACTCGCCGAATCTCCACCCCAAAAGTTTGGCAAATGTGTTTCTATATTTGTATGGGAGGAAGTTCCCAATCCTTTGCTAATACCGGTTCTATGGTTACTTGTGTTAATAACTTTCCCGAAAGGCGTGGCGTTGTTCTCGGTCTTTTGAAGGGATACATCGGGCTAAGCGGCGCCATAATCACACAGCTCTTCCACGCATTCTACGGCGGCGACACTAAATCCCTCATTCTCTTCATCGGATGGCTTCCGGCTGCAATTTCCTTCGCATTCTTACGAACAGTTCGTATCATGAAAGTCATCCGACAGCCAAATGAGCTCAAAGTTTTCTATAATTTCCTCTACATTTCTCTTCTCCTCGCCGGATTCCTCATGCTAATGATCATTGTACAAAGCAAGACAGAGTTCACTCAAAACCAATATGGTGGCAGCGCGGCTGCCATAGTCGTTCTTCTCCTCCTACCACTCGCCGTTGTCACTATCGAAGAATGCAATCTCCAGAAACTTAAAACCAAATCTCCAAATTCTTCTGTTCAAATCATAACTGAGAAACTCCCAAAAACAGAGCATTCAAAACAAAAAGAACCATCGTGTTGGACAACAATTTTCAACCCACCACAACGAGGCGAGGATTTCACAATTCTCCAAGCTGTCTTCAGTGTCGACATGCTGATTTTATTCATATCTGTAATTTGTGGCGCTGGTGGAACGTTAACGGCGGTCGACAATTTGGGTCAAATCGGAATGTCTTTGGGATACCCAAAAAGAAGCATTAGCACATTTGTATCATTAGTCAGTATTTGGAATTACCTCGGTCGAGTTGTCTCTGGTTTCGTCTCTGAAATCGTTTTGATAAAATACAAATTCCCTCGTCCTTTAATGCTTTCTCTGAACCTTCTTTTATCCTGTGTCGGCTACTTGGTCATCGCATTTGACGTCCCAAATGGATTATATGTTGCTTCCATCGTAATCGGATTCTGTGTTGGAGCACAGTGGCCGTTGATTTACGCCATAATTTCAGAGATTTTTGGGTTGAAATATTACTCAACGCTTTACAATTTCGGGACGGTTGCGATGCCAATTGGACTTTATATTATGAATGTGAAAGTGGCTGGGAACTTCTACGACAGAGAAGCAGAGAAACAGCTAAAAGCAAAAGGGATTATACGAAAAGCAGGGGAAGACTTAAAGTGCTATGGAGGGGAATGCTTTAAGCTTTCGTTCATCGTAATCACTGCAGTTACTTTGATGGGTATGTTTATTTCATTGATTTTAGTGATTAGAACAAGAAGTTTTTACAAAAGTGATATTTATAAAAAGTTTAGAGATGAAGCCGAAACAGAGGTGGCCGGAAATGAGGCTGTGGCGACGGCGGGAGGAGCAGAAGAGAGAGAGAAAATTATAATGCAAAGAAGAAGTTGA

Protein sequence

MPPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVNNFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRIMKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLAVVTIEECNLQKLKTKSPNSSVQIITEKLPKTEHSKQKEPSCWTTIFNPPQRGEDFTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWNYLGRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVGAQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGIIRKAGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEAVATAGGAEEREKIIMQRRS*
Homology
BLAST of CsGy5G026240 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 3.3e-20
Identity = 112/472 (23.73%), Postives = 207/472 (43.86%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYIC-MGGSSQSFANTGSMVTCV 61
           P  VVL   A M F GY + WL +T  I+ P  + + F  C + G S  + NT   + C+
Sbjct: 106 PLSVVLFAAAAMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLSICWFNTACFILCI 165

Query: 62  NNFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVR 121
            +FP  R + L L   + G+S A+ +  F+A     +   +L    +P  +SFA L  V 
Sbjct: 166 RHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYPVL 225

Query: 122 IMKVI-------RQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIV 181
               +        + ++  VF   L +  ++  F +L  +  S T   +  + G+   +V
Sbjct: 226 TKPSLDTTPDYDSRRHDSHVF-TILNVLAVITSFHLL--LSSSSTSSARLNFIGAVVLLV 285

Query: 182 VLLLLPLAVVT----IEECNLQKLKTKSPNSSVQIITEKLPKTEHSKQK--EPSCWTTIF 241
             L  PL V      +   N +     S    + I   K  KT  S +   E        
Sbjct: 286 FPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKEG 345

Query: 242 NPPQRGEDFTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVS 301
           N  + G++ +    +  ++  + +I+  CG    L   +NLGQI  SLG   ++ +T V+
Sbjct: 346 NTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG---QNSTTLVT 405

Query: 302 LVSIWNYLGRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGY--LVIAFDVPNGLYVAS 361
           + S +++ GR++S    + +  +++  R    ++ LL + + +  L ++      L  A+
Sbjct: 406 IYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTAT 465

Query: 362 IVIGFCVGAQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEK 421
            +IG   G  +    +I S++FG       +N     +PIG  +    +A + Y+  A  
Sbjct: 466 ALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAASIYEANA-- 525

Query: 422 QLKAKGIIRKAGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFY 458
              +  I     + + C G +C+  +F+    ++++G+  SL L IRT+  Y
Sbjct: 526 ---SPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of CsGy5G026240 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 2.5e-04
Identity = 42/160 (26.25%), Postives = 80/160 (50.00%), Query Frame = 0

Query: 281 SISTFVSLVSIWNYLGRVVSGFVSEIVLIKYKFPRPLMLS-LNLLLSCVGYLV-----IA 340
           S ST VSL + ++ + R+V GF SE   ++    RP++LS + L+ +C+  +V       
Sbjct: 311 SFSTHVSLFATFSTVSRLVVGFSSE--AMESHVSRPVLLSVIALVAACIHLMVPSGIFTV 370

Query: 341 FDVPNGLYVASIVIGFCVGAQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKV 400
           FD      V +IV GF  G+ + L+  I+++++G+    T++    +A+ +G     +  
Sbjct: 371 FDNAKYFSVVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIWGSFILALAVGSLGYGLLF 430

Query: 401 AGNFYDREAEKQLKAKGIIRKAGEDLKCYGGECFKLSFIV 435
           A   YD  +E  + +   +        C G  C+ L+F++
Sbjct: 431 A-KVYDAASEVGVGSMSQV--------CSGVHCYGLTFVI 459

BLAST of CsGy5G026240 vs. NCBI nr
Match: XP_031741165.1 (uncharacterized protein LOC101205265 [Cucumis sativus] >KAE8648845.1 hypothetical protein Csa_009229 [Cucumis sativus])

HSP 1 Score: 961 bits (2485), Expect = 0.0
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MPPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCV 60
           MPPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCV
Sbjct: 86  MPPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCV 145

Query: 61  NNFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVR 120
           NNFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVR
Sbjct: 146 NNFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVR 205

Query: 121 IMKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPL 180
           IMKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPL
Sbjct: 206 IMKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPL 265

Query: 181 AVVTIEECNLQKLKTKSPNSSVQIITEKLPKTEHSKQKEPSCWTTIFNPPQRGEDFTILQ 240
           AVVTIEECNLQKLKTKSPNSSVQIITEKLPKTEHSKQKEPSCWTTIFNPPQRGEDFTILQ
Sbjct: 266 AVVTIEECNLQKLKTKSPNSSVQIITEKLPKTEHSKQKEPSCWTTIFNPPQRGEDFTILQ 325

Query: 241 AVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWNYLGRVVS 300
           AVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWNYLGRVVS
Sbjct: 326 AVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWNYLGRVVS 385

Query: 301 GFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVGAQWPLIY 360
           GFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVGAQWPLIY
Sbjct: 386 GFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVGAQWPLIY 445

Query: 361 AIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGIIRKAGEDL 420
           AIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGIIRKAGEDL
Sbjct: 446 AIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGIIRKAGEDL 505

Query: 421 KCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEAVA 480
           KCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEAVA
Sbjct: 506 KCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEAVA 565

Query: 481 TAGGAEEREKIIMQRRS 497
           TAGGAEEREKIIMQRRS
Sbjct: 566 TAGGAEEREKIIMQRRS 582

BLAST of CsGy5G026240 vs. NCBI nr
Match: XP_031741159.1 (uncharacterized protein LOC101205029 [Cucumis sativus] >KAE8648846.1 hypothetical protein Csa_008243 [Cucumis sativus])

HSP 1 Score: 872 bits (2253), Expect = 0.0
Identity = 443/496 (89.31%), Postives = 470/496 (94.76%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           PPWV+LLIGA+MNFFGYFMIWL VTRRI  PKVWQMC YICMGGSSQ+FANTG++VTCVN
Sbjct: 87  PPWVILLIGALMNFFGYFMIWLSVTRRIFAPKVWQMCLYICMGGSSQNFANTGALVTCVN 146

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPERRG+VLGLLKGY+GLSGAIITQLFHAFYGGDTKS IL IGWLPAAIS AFLRTVRI
Sbjct: 147 NFPERRGIVLGLLKGYVGLSGAIITQLFHAFYGGDTKSFILLIGWLPAAISLAFLRTVRI 206

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
           MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA
Sbjct: 207 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 266

Query: 182 VVTIEECNLQKLKTKSPNSSVQIITEKLPKTEHSKQKEPSCWTTIFNPPQRGEDFTILQA 241
           VVT EE NL KLKTKSPN SVQIITE+LPKTEH +QKEPSCWTTIFNPPQRGEDFTILQA
Sbjct: 267 VVTTEEYNLWKLKTKSPNPSVQIITEQLPKTEHPEQKEPSCWTTIFNPPQRGEDFTILQA 326

Query: 242 VFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWNYLGRVVSG 301
           VFSVDMLILF+SVICG GG LTA++NLGQIGMSLGYPK+SISTFVSLVSIWNYLGRVVSG
Sbjct: 327 VFSVDMLILFLSVICGTGGQLTAIENLGQIGMSLGYPKKSISTFVSLVSIWNYLGRVVSG 386

Query: 302 FVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVGAQWPLIYA 361
           F SEIVLIKYKFPRPL+LSL LLLSC+G+L+IAFDVPNGLYVASIVIGFC+GAQWP+IYA
Sbjct: 387 FASEIVLIKYKFPRPLILSLTLLLSCIGHLMIAFDVPNGLYVASIVIGFCLGAQWPMIYA 446

Query: 362 IISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGIIRKAGEDLK 421
           IISEIFGLKYYSTLYNFG  A+PIGLYIMNVKV G  YDREAEKQLKAKGIIRKAGE+LK
Sbjct: 447 IISEIFGLKYYSTLYNFGAAAIPIGLYIMNVKVTGKLYDREAEKQLKAKGIIRKAGEELK 506

Query: 422 CYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEAVAT 481
           C+G ECFKLSFI+ITAVTL+GMFISLILVIRTRSFY SDIYKKFR+EAE EVAGNEAVAT
Sbjct: 507 CFGRECFKLSFIIITAVTLVGMFISLILVIRTRSFYISDIYKKFREEAEKEVAGNEAVAT 566

Query: 482 AGGAEEREKIIMQRRS 497
           AG AEE EK+I+QRRS
Sbjct: 567 AGAAEETEKVIIQRRS 582

BLAST of CsGy5G026240 vs. NCBI nr
Match: XP_008446819.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0034695.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK09246.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 821 bits (2121), Expect = 7.76e-296
Identity = 421/496 (84.88%), Postives = 453/496 (91.33%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           PPWVVL IGAV+NFFGYFMIWL VTRRISTPKVWQMC YIC+G +SQSFANTGS+VTCV 
Sbjct: 87  PPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIGANSQSFANTGSLVTCVK 146

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RGVVLG+LKGY+GLSGAIITQLFHAFYG DTKSLIL IGWLPAAISFA LRT+RI
Sbjct: 147 NFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFASLRTIRI 206

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
           MKVIRQPNELKVFYNFLYISL LAGFLMLMIIV+SK EF QN+YGGSAA +++LL LPL+
Sbjct: 207 MKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKKEFNQNEYGGSAAVVLLLLFLPLS 266

Query: 182 VVTIEECNLQKLKT---KSPNSSVQIITEKLPKTEHSKQ---KEPSCWTTIFNPPQRGED 241
           VV IEE NL KLKT   KSPN SVQI+TEKLPKTEH KQ   KEPSCWTTIFNPPQRGED
Sbjct: 267 VVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKTEHPKQLEQKEPSCWTTIFNPPQRGED 326

Query: 242 FTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWNYL 301
           FTILQA+FS DMLILFI+ ICG GGTLTA+DNLGQIGMSLGYPKRSISTFV+LVSIWNYL
Sbjct: 327 FTILQALFSFDMLILFIAAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYL 386

Query: 302 GRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVGAQ 361
           GRV SGF+SEIVL KYKFPRPL+LSL LLLSCVG+L+IAFDVPNGLYVASIVIGFC GAQ
Sbjct: 387 GRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQ 446

Query: 362 WPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGIIRK 421
           WPLI+AIISE+FGLKYYSTLYNFG+VA PIGLYI+NVKVAGNFYDREAEKQL AKGIIRK
Sbjct: 447 WPLIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLSAKGIIRK 506

Query: 422 AGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAET-EVA 481
           AGE+LKC+GGECFKLSFIVITAVTL+GM +SL+LVIRTRSFYKSDIYKKFRDEAET EVA
Sbjct: 507 AGEELKCFGGECFKLSFIVITAVTLLGMLVSLVLVIRTRSFYKSDIYKKFRDEAETKEVA 566

Query: 482 GNEAVATAGGAEEREK 490
           GN  +  AG  EER K
Sbjct: 567 GNAVIEVAGVPEERRK 582

BLAST of CsGy5G026240 vs. NCBI nr
Match: XP_022956604.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 816 bits (2108), Expect = 9.55e-294
Identity = 414/503 (82.31%), Postives = 454/503 (90.26%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           PPWVVL IGAVMNFFGYFMIWL VTR+I+ PKVWQMC YIC+G +SQSFANTGS+VTCV 
Sbjct: 87  PPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVWQMCLYICIGANSQSFANTGSLVTCVK 146

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RGVVLG+LKGY+GLSGAIITQLFHAFYGGDTKSLIL IGWLPAAISFAFLRT+RI
Sbjct: 147 NFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTIRI 206

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
           MKVIRQPNELKVFYNFLYISL LAGFLMLMIIV+SKTEF+Q+ YGGSAA +++LL LPLA
Sbjct: 207 MKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKTEFSQSGYGGSAAVVLLLLFLPLA 266

Query: 182 VVTIEECNLQKLKT-------KSPNSSVQIITEKLPKTEHSK-------QKEPSCWTTIF 241
           VV IEE NL  LKT       +SPN  VQI+TEKLPKTEH K       QKEPSCWTTIF
Sbjct: 267 VVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEKLPKTEHQKHIPKTEHQKEPSCWTTIF 326

Query: 242 NPPQRGEDFTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVS 301
           NPP+RGEDFTILQA+FSVDMLILF++ ICG GGTLTA+DNLGQIGMSLGYPK+SISTFV+
Sbjct: 327 NPPERGEDFTILQALFSVDMLILFLAAICGVGGTLTAIDNLGQIGMSLGYPKQSISTFVT 386

Query: 302 LVSIWNYLGRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIV 361
           LVSIWNYLGRV SGFVSEIVL KYKFPRPLMLS+ LL+SCVG+L+IAFDVPNGLYVASIV
Sbjct: 387 LVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIV 446

Query: 362 IGFCVGAQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQL 421
           IGFC GAQWPLI+AIISE+FGLKYYSTLYNFG+VA PIGLY++NVKVAGNFYDREAEKQL
Sbjct: 447 IGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQL 506

Query: 422 KAKGIIRKAGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRD 481
           +AKGI+RK GE+LKC+GGECFKLSF+VIT VTL+GMF+SLILVIRTRSFYKSDIYKKFRD
Sbjct: 507 RAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLGMFVSLILVIRTRSFYKSDIYKKFRD 566

Query: 482 EAETEVAGNEAVATAGGAEEREK 490
           EAETE+AGN AV  AG  EE  K
Sbjct: 567 EAETEMAGNGAVVAAGPPEETRK 589

BLAST of CsGy5G026240 vs. NCBI nr
Match: XP_038893353.1 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 814 bits (2103), Expect = 5.30e-293
Identity = 413/500 (82.60%), Postives = 455/500 (91.00%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           PPWVVL IGA++NFFGYFMIWL VTRRIS PKVWQMC YIC+G +SQSFANTGS+VTCV 
Sbjct: 87  PPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVWQMCVYICIGANSQSFANTGSLVTCVK 146

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RGVVLG+LKGY+GLSGAIITQLFHAFYG DTKSLIL IGWLPAAISFAFLRT+RI
Sbjct: 147 NFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRI 206

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
           +KVIR PNELKVFYNFLYISL LAGFLMLMII++SKTEFTQN+YGGSAA ++VLL LPLA
Sbjct: 207 IKVIRHPNELKVFYNFLYISLGLAGFLMLMIIIESKTEFTQNEYGGSAAVVLVLLFLPLA 266

Query: 182 VVTIEECNLQKLKT---KSPNSSVQIITEKLPKTEHSKQ------KEPSCWTTIFNPPQR 241
           VV IEE NL KLKT   KS N  VQI+TE+LPKTEH KQ      KEPSCWTT+F+PP+R
Sbjct: 267 VVIIEEYNLWKLKTAFIKSSNPCVQIVTEQLPKTEHPKQTPKQEQKEPSCWTTVFSPPER 326

Query: 242 GEDFTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIW 301
           GEDFTILQA+FS+DMLILF+S  CG GGTLTA+DNLGQIGMSLGYPKRSISTFVSLVSIW
Sbjct: 327 GEDFTILQALFSIDMLILFLSATCGVGGTLTAIDNLGQIGMSLGYPKRSISTFVSLVSIW 386

Query: 302 NYLGRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCV 361
           NYLGR+ SGFVSEIV+ KYKFPRPL+LSL LLLSCVG+L+IAFDVPNGLYVAS+VIGFC 
Sbjct: 387 NYLGRIASGFVSEIVVTKYKFPRPLILSLTLLLSCVGHLMIAFDVPNGLYVASVVIGFCF 446

Query: 362 GAQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGI 421
           GAQWP+I+AIISE+FGLKYYSTLYNFG+VA PIGLYI+NVKVAGNFYDREAEKQL+AKGI
Sbjct: 447 GAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLRAKGI 506

Query: 422 IRKAGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAETE 481
            RKAG+ LKC+GGECFKLSFIVITAVTL+G FISLILVIRT+SFYKSDIYKKFRDEAETE
Sbjct: 507 TRKAGKKLKCFGGECFKLSFIVITAVTLLGTFISLILVIRTKSFYKSDIYKKFRDEAETE 566

Query: 482 VAGNEAVATAGGAEEREKII 492
           VAGN AVA AG  EE +K++
Sbjct: 567 VAGNGAVAPAGAGEETKKMM 586

BLAST of CsGy5G026240 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 821 bits (2121), Expect = 3.76e-296
Identity = 421/496 (84.88%), Postives = 453/496 (91.33%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           PPWVVL IGAV+NFFGYFMIWL VTRRISTPKVWQMC YIC+G +SQSFANTGS+VTCV 
Sbjct: 87  PPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIGANSQSFANTGSLVTCVK 146

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RGVVLG+LKGY+GLSGAIITQLFHAFYG DTKSLIL IGWLPAAISFA LRT+RI
Sbjct: 147 NFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFASLRTIRI 206

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
           MKVIRQPNELKVFYNFLYISL LAGFLMLMIIV+SK EF QN+YGGSAA +++LL LPL+
Sbjct: 207 MKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKKEFNQNEYGGSAAVVLLLLFLPLS 266

Query: 182 VVTIEECNLQKLKT---KSPNSSVQIITEKLPKTEHSKQ---KEPSCWTTIFNPPQRGED 241
           VV IEE NL KLKT   KSPN SVQI+TEKLPKTEH KQ   KEPSCWTTIFNPPQRGED
Sbjct: 267 VVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKTEHPKQLEQKEPSCWTTIFNPPQRGED 326

Query: 242 FTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWNYL 301
           FTILQA+FS DMLILFI+ ICG GGTLTA+DNLGQIGMSLGYPKRSISTFV+LVSIWNYL
Sbjct: 327 FTILQALFSFDMLILFIAAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYL 386

Query: 302 GRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVGAQ 361
           GRV SGF+SEIVL KYKFPRPL+LSL LLLSCVG+L+IAFDVPNGLYVASIVIGFC GAQ
Sbjct: 387 GRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQ 446

Query: 362 WPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGIIRK 421
           WPLI+AIISE+FGLKYYSTLYNFG+VA PIGLYI+NVKVAGNFYDREAEKQL AKGIIRK
Sbjct: 447 WPLIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLSAKGIIRK 506

Query: 422 AGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAET-EVA 481
           AGE+LKC+GGECFKLSFIVITAVTL+GM +SL+LVIRTRSFYKSDIYKKFRDEAET EVA
Sbjct: 507 AGEELKCFGGECFKLSFIVITAVTLLGMLVSLVLVIRTRSFYKSDIYKKFRDEAETKEVA 566

Query: 482 GNEAVATAGGAEEREK 490
           GN  +  AG  EER K
Sbjct: 567 GNAVIEVAGVPEERRK 582

BLAST of CsGy5G026240 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 821 bits (2121), Expect = 3.76e-296
Identity = 421/496 (84.88%), Postives = 453/496 (91.33%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           PPWVVL IGAV+NFFGYFMIWL VTRRISTPKVWQMC YIC+G +SQSFANTGS+VTCV 
Sbjct: 87  PPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIGANSQSFANTGSLVTCVK 146

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RGVVLG+LKGY+GLSGAIITQLFHAFYG DTKSLIL IGWLPAAISFA LRT+RI
Sbjct: 147 NFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFASLRTIRI 206

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
           MKVIRQPNELKVFYNFLYISL LAGFLMLMIIV+SK EF QN+YGGSAA +++LL LPL+
Sbjct: 207 MKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKKEFNQNEYGGSAAVVLLLLFLPLS 266

Query: 182 VVTIEECNLQKLKT---KSPNSSVQIITEKLPKTEHSKQ---KEPSCWTTIFNPPQRGED 241
           VV IEE NL KLKT   KSPN SVQI+TEKLPKTEH KQ   KEPSCWTTIFNPPQRGED
Sbjct: 267 VVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKTEHPKQLEQKEPSCWTTIFNPPQRGED 326

Query: 242 FTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWNYL 301
           FTILQA+FS DMLILFI+ ICG GGTLTA+DNLGQIGMSLGYPKRSISTFV+LVSIWNYL
Sbjct: 327 FTILQALFSFDMLILFIAAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYL 386

Query: 302 GRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVGAQ 361
           GRV SGF+SEIVL KYKFPRPL+LSL LLLSCVG+L+IAFDVPNGLYVASIVIGFC GAQ
Sbjct: 387 GRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQ 446

Query: 362 WPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGIIRK 421
           WPLI+AIISE+FGLKYYSTLYNFG+VA PIGLYI+NVKVAGNFYDREAEKQL AKGIIRK
Sbjct: 447 WPLIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLSAKGIIRK 506

Query: 422 AGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAET-EVA 481
           AGE+LKC+GGECFKLSFIVITAVTL+GM +SL+LVIRTRSFYKSDIYKKFRDEAET EVA
Sbjct: 507 AGEELKCFGGECFKLSFIVITAVTLLGMLVSLVLVIRTRSFYKSDIYKKFRDEAETKEVA 566

Query: 482 GNEAVATAGGAEEREK 490
           GN  +  AG  EER K
Sbjct: 567 GNAVIEVAGVPEERRK 582

BLAST of CsGy5G026240 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 816 bits (2108), Expect = 4.62e-294
Identity = 414/503 (82.31%), Postives = 454/503 (90.26%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           PPWVVL IGAVMNFFGYFMIWL VTR+I+ PKVWQMC YIC+G +SQSFANTGS+VTCV 
Sbjct: 87  PPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVWQMCLYICIGANSQSFANTGSLVTCVK 146

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RGVVLG+LKGY+GLSGAIITQLFHAFYGGDTKSLIL IGWLPAAISFAFLRT+RI
Sbjct: 147 NFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTIRI 206

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
           MKVIRQPNELKVFYNFLYISL LAGFLMLMIIV+SKTEF+Q+ YGGSAA +++LL LPLA
Sbjct: 207 MKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKTEFSQSGYGGSAAVVLLLLFLPLA 266

Query: 182 VVTIEECNLQKLKT-------KSPNSSVQIITEKLPKTEHSK-------QKEPSCWTTIF 241
           VV IEE NL  LKT       +SPN  VQI+TEKLPKTEH K       QKEPSCWTTIF
Sbjct: 267 VVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEKLPKTEHQKHIPKTEHQKEPSCWTTIF 326

Query: 242 NPPQRGEDFTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVS 301
           NPP+RGEDFTILQA+FSVDMLILF++ ICG GGTLTA+DNLGQIGMSLGYPK+SISTFV+
Sbjct: 327 NPPERGEDFTILQALFSVDMLILFLAAICGVGGTLTAIDNLGQIGMSLGYPKQSISTFVT 386

Query: 302 LVSIWNYLGRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIV 361
           LVSIWNYLGRV SGFVSEIVL KYKFPRPLMLS+ LL+SCVG+L+IAFDVPNGLYVASIV
Sbjct: 387 LVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIV 446

Query: 362 IGFCVGAQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQL 421
           IGFC GAQWPLI+AIISE+FGLKYYSTLYNFG+VA PIGLY++NVKVAGNFYDREAEKQL
Sbjct: 447 IGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQL 506

Query: 422 KAKGIIRKAGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRD 481
           +AKGI+RK GE+LKC+GGECFKLSF+VIT VTL+GMF+SLILVIRTRSFYKSDIYKKFRD
Sbjct: 507 RAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLGMFVSLILVIRTRSFYKSDIYKKFRD 566

Query: 482 EAETEVAGNEAVATAGGAEEREK 490
           EAETE+AGN AV  AG  EE  K
Sbjct: 567 EAETEMAGNGAVVAAGPPEETRK 589

BLAST of CsGy5G026240 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 808 bits (2086), Expect = 7.67e-291
Identity = 408/497 (82.09%), Postives = 450/497 (90.54%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           PPWVVL IGAVMNFFGYFMIWL VT +IS PKVWQMC YIC+G +SQSFANTGS+VTCV 
Sbjct: 87  PPWVVLSIGAVMNFFGYFMIWLAVTGKISAPKVWQMCLYICIGANSQSFANTGSLVTCVK 146

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RGVVLG+LKGY+GLSGAIITQLFHAFYGGDTKSLIL IGWLPAAISFAFLRT+RI
Sbjct: 147 NFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTIRI 206

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
           MKVIRQPNELKVFYNFLYISL LAGFLMLMII++SKTEF+Q+ YGGSAA +++LL LPLA
Sbjct: 207 MKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKTEFSQSGYGGSAAVVLLLLFLPLA 266

Query: 182 VVTIEECNLQKLKT--------KSPNSSVQIITEKLPKTEHSKQKEPSCWTTIFNPPQRG 241
           VV IEE NL  LKT        +SPN  VQI+TEK+PKTEH  QK+PSCWTTIFNPP+RG
Sbjct: 267 VVIIEEYNLWNLKTAAATAAAIRSPNHPVQIVTEKVPKTEH--QKQPSCWTTIFNPPERG 326

Query: 242 EDFTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWN 301
           EDFTILQA+FSVDM ILF++ ICG GGTLTA+DNLGQIGMSLGYPK+SISTFV+LVSIWN
Sbjct: 327 EDFTILQALFSVDMFILFLAAICGVGGTLTAIDNLGQIGMSLGYPKQSISTFVTLVSIWN 386

Query: 302 YLGRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVG 361
           YLGRV SGFVSEIVL KYKFPRPLMLS+ LL+SCVG+L+IAFDVPNGLYVASIVIGFC G
Sbjct: 387 YLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIVIGFCFG 446

Query: 362 AQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGII 421
           AQWPLI+AIISE+FGLKYYSTLYNFG+VA PIGLY++NVKVAGNFYDREAEKQL+AKGI+
Sbjct: 447 AQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIV 506

Query: 422 RKAGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEV 481
           RK GE+LKC+GGECFKLSF+VIT VTL+GMF+SLILVIRTRSFYKSDIYKKFRDEAETE+
Sbjct: 507 RKTGEELKCFGGECFKLSFLVITGVTLLGMFVSLILVIRTRSFYKSDIYKKFRDEAETEI 566

Query: 482 AGNEAVATAGGAEEREK 490
           AGN AV  A   EE  K
Sbjct: 567 AGNGAVVAAEPPEETRK 581

BLAST of CsGy5G026240 vs. ExPASy TrEMBL
Match: A0A6J1FZF2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326 PE=4 SV=1)

HSP 1 Score: 775 bits (2002), Expect = 6.17e-278
Identity = 394/494 (79.76%), Postives = 438/494 (88.66%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           PPWVVL IGAV+NFFGYFMIWL VT+RISTPKVWQMC YIC+G +SQSF NTGS+VTCV 
Sbjct: 92  PPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMCLYICVGANSQSFVNTGSLVTCVK 151

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RGVVLG+LKGY+GLSGAIITQLFHAFYG DTKSLIL IGWLPAAISFAFLRT+RI
Sbjct: 152 NFPESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIGWLPAAISFAFLRTIRI 211

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
           MKVIRQPNELKVFYNFLYISL LAGFLMLMII++SK  F+Q ++GGSAA +++LL LPLA
Sbjct: 212 MKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKLIFSQAEFGGSAAVVLLLLFLPLA 271

Query: 182 VVTIEECNLQKLKTKS--PNSSVQIITEKL--PKTEHSKQ----KEPSCWTTIFNPPQRG 241
           VV +EE NL KLK+ +  P + +QI+TEK   PKTE  KQ    K+PSCWTTIF+PP+RG
Sbjct: 272 VVIVEEYNLSKLKSAAINPPNPLQILTEKPNPPKTEPQKQTRQQKQPSCWTTIFSPPERG 331

Query: 242 EDFTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWN 301
           EDFTILQA+FS+DM ILF S ICG GGTLTA+DNLGQIGMSLGYPKRSISTFV+LVSIWN
Sbjct: 332 EDFTILQALFSLDMAILFFSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWN 391

Query: 302 YLGRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVG 361
           YLGRV SGFVSEIVL KYKFPRPLMLSL LLLSCVG+L+IAF VPNGLYVASIVIGFC G
Sbjct: 392 YLGRVASGFVSEIVLTKYKFPRPLMLSLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFG 451

Query: 362 AQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGII 421
           AQWPL++AIISE+FGLKYYSTLYNFG+VA PIGLY++NVKVAGN YD+EA KQL AKG++
Sbjct: 452 AQWPLLFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLV 511

Query: 422 RKAGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEV 481
           RK GE+LKCYGGECFK SF+VIT VTL+GMF+SLILVIRTR FYKSDIYKKFRDEAE EV
Sbjct: 512 RKTGEELKCYGGECFKRSFLVITGVTLLGMFVSLILVIRTRGFYKSDIYKKFRDEAEAEV 571

Query: 482 AGNEAVATAGGAEE 487
            GN AV  AG  EE
Sbjct: 572 VGNGAVTAAGAPEE 585

BLAST of CsGy5G026240 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 672.9 bits (1735), Expect = 1.9e-193
Identity = 344/510 (67.45%), Postives = 410/510 (80.39%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           PPW +LLIGA++NFFGYFMIWL VT RIS P+VW MC YIC+G +SQSFANTGS+VTCV 
Sbjct: 84  PPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANTGSLVTCVK 143

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RGVVLG+LKGY+GLSGAIITQL+ AFYG DTK LIL IGWLPA +SFAFLRT+RI
Sbjct: 144 NFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSFAFLRTIRI 203

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
           MKV RQ NELKVFYNFLYISL LA FLM++II+   + FTQ+++GGSAA ++VLLLLP+ 
Sbjct: 204 MKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVIVLLLLPII 263

Query: 182 VVTIEECNLQKLKTKSPN--SSVQIITEKLPKTEHS---------------KQKEPSCWT 241
           VV +EE  L K K  + N  + + ++TEK PK + S               K K PSCWT
Sbjct: 264 VVILEEKKLWKEKQVALNDPAPINVVTEK-PKLDSSEFKDDDGEESKEVVEKVKTPSCWT 323

Query: 242 TIFNPPQRGEDFTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSIST 301
           T+FNPP+RG+D+TILQA+FSVDMLILF++ ICG GGTLTA+DNLGQIG SLGYPKRS+ST
Sbjct: 324 TVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGYPKRSVST 383

Query: 302 FVSLVSIWNYLGRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVA 361
           FVSLVSIWNY GRVVSG VSEI LIKYKFPRPLML++ LLLSC G+L+IAF+VP GLYVA
Sbjct: 384 FVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYVA 443

Query: 362 SIVIGFCVGAQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAE 421
           S++IGFC GAQWPL++AIISEIFGLKYYSTLYNFG+VA PIG Y++NV+VAG  YD EA 
Sbjct: 444 SVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGYLYDVEAG 503

Query: 422 KQLKAKGIIRKAGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKK 481
           KQ KA G  R  G+DL C G  CFKLSFI+I AVTL G+ +S++LVIRT+ FYKSDIYKK
Sbjct: 504 KQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFYKSDIYKK 563

Query: 482 FRDE---AETEVAGNEAVATAGGAEEREKI 492
           FR++   AE E+A   A  +    E+++ +
Sbjct: 564 FREKALAAEMEMAAPAAARSTVAKEDKDDV 592

BLAST of CsGy5G026240 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 555.8 bits (1431), Expect = 3.4e-158
Identity = 280/482 (58.09%), Postives = 354/482 (73.44%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           P W VL IG+ MNF GYFMIWL VT +++ PKVWQMC YIC+G +SQ+FANTG++VTCV 
Sbjct: 80  PTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALVTCVK 139

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RGV+LGLLKGY+GLSGAI TQL+ A YG D+KSLIL I WLPAA+S  F+  +R 
Sbjct: 140 NFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVSLVFVYLIRE 199

Query: 122 MKVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLA 181
            KV+RQ NEL VFY FLYIS+ LA FLM M I + +  F++  Y  SA     LL +PL 
Sbjct: 200 KKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLFVPLT 259

Query: 182 VVT---IEECNLQKL--------KTKSPNSSVQIITEKLPKTEHSKQKEPSCWTTIFNPP 241
           V     +E  N+ KL        K + P   + +  +K  K    +++  SC++T+F+PP
Sbjct: 260 VSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETKSCFSTVFSPP 319

Query: 242 QRGEDFTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVS 301
            RGED+TILQA+ S DM+ILF++  CG G +LTAVDNLGQIG SLGYP  ++S+FVSLVS
Sbjct: 320 PRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSFVSLVS 379

Query: 302 IWNYLGRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGF 361
           IWNY GRV SGFVSE +L KYK PRPLM++L LLLSC G+L+IAF VP  +Y+ASI++GF
Sbjct: 380 IWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIASILMGF 439

Query: 362 CVGAQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAK 421
             GAQ PL++AIISE+FGLKYYSTL+N G +A P+G YI+NV+V G  YD+EA KQL A+
Sbjct: 440 SFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALKQLTAR 499

Query: 422 GIIRKAGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAE 473
           G+ RK  +DL C G +C+KL F+++ AVT  G  +SL L IRTR FYK DIYKKFR+  E
Sbjct: 500 GLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKKFRESPE 559

BLAST of CsGy5G026240 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 7.3e-76
Identity = 176/508 (34.65%), Postives = 279/508 (54.92%), Query Frame = 0

Query: 1   MPPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCV 60
           +P W  LL+GAV N  GY  +WL VT R     +W MC  I +G + +++ NTG++V+ V
Sbjct: 78  LPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVSGV 137

Query: 61  NNFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFA---FLR 120
            NFP+ RG V+G+LKG+ GL GAII+Q++   +  +  SLIL +   PA +      F+R
Sbjct: 138 QNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFFIR 197

Query: 121 TVRIMKVIRQPNELKVFYNFLY-ISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLL 180
            V   K IR  +     + F+Y + LLLA +LM ++++Q     + N        + V+L
Sbjct: 198 PVGGHKQIRPTDGAS--FTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVIL 257

Query: 181 LLPLAVV--------------TIEECNLQKLKTKSPNSSV------QIITEK------LP 240
           ++P+ V               TIEE  + K + + P          ++  EK      LP
Sbjct: 258 VVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLLP 317

Query: 241 KTEHSKQ------------KEPSCWTTIFNPPQRGEDFTILQAVFSVDMLILFISVICGA 300
            +E  K+             E +        P RGEDFT+ QA+   D  ++F S++ G+
Sbjct: 318 ASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLGS 377

Query: 301 GGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWNYLGRVVSGFVSEIVLIKYKFPRPLM 360
           G  LT +DNLGQ+  SLGY    +   VS++SIWN+LGR+  G+ SE+V+  Y +PRP+ 
Sbjct: 378 GSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYFSELVVRDYAYPRPVA 437

Query: 361 LSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVGAQWPLIYAIISEIFGLKYYSTLYNF 420
           +++  L+  VG++  A+  P  +Y+ +++IG   GA W ++ A  SE+FGLK +  LYNF
Sbjct: 438 MAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYNF 497

Query: 421 GTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGIIRKAGEDLKCYGGECFKLSFIVITAV 467
            T+A P G  + +  +A + YDREAE+Q  A G +    + L+C G  CF L+ ++++  
Sbjct: 498 LTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPDDALRCNGSICFFLTSLIMSGF 557

BLAST of CsGy5G026240 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 4.8e-75
Identity = 157/462 (33.98%), Postives = 259/462 (56.06%), Query Frame = 0

Query: 3   PWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVNN 62
           PWVVL +GA+  F GYF+IW  VT  I  P V  MC ++ +   SQ+F NT ++V+ V N
Sbjct: 87  PWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCLFMFLAAQSQTFFNTANVVSAVEN 146

Query: 63  FPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRIM 122
           F +  G  +G++KG++GLSGAI+ QL+     GD  S IL +   P  +S   +  VRI 
Sbjct: 147 FADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPASFILLLAVTPTVLSLLVMPLVRIY 206

Query: 123 KVIRQPNELKVFYNFLYISLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLLPLAV 182
           +     ++ K       +SL++A +LM++II+  K  F  + +      + +L++L L +
Sbjct: 207 ET-SVADDKKHLNGLSAVSLIIAAYLMIIIIL--KNTFGLSSWANIVTLVCLLVMLALPL 266

Query: 183 VTIEECNLQKLKTKSPNSSVQIITEKLPKTEHSKQKEPSCWTTIFNPPQRGEDFTILQAV 242
           +         ++   P+    +I+     T  ++  E              E+  +LQA+
Sbjct: 267 LIARRAQRDGMEKTVPHDYSPLISSPKATTSGNQSSEGDSKV----EAGLSENLNLLQAM 326

Query: 243 FSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIWNYLGRVVSGF 302
             +   +LF+++ICG G  L+ ++N+ QIG SL Y    I++ VSL SIWN+LGR  +G+
Sbjct: 327 KKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGY 386

Query: 303 VSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCVGAQWPLIYAI 362
            S+ +L K  +PRPL+++  L    +G+L+IA      LYV S+++G C G+QW L+  I
Sbjct: 387 ASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTI 446

Query: 363 ISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGIIRKAGEDLKC 422
            SE+FG+++  T++N  +VA PIG YI +V++ G  YD+ A            +GE   C
Sbjct: 447 TSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKTA------------SGEGNTC 506

Query: 423 YGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKSDIYKK 465
           YG  CF+LSFI++ +V   G  ++++L  RT++ Y+  + K+
Sbjct: 507 YGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILVKR 529

BLAST of CsGy5G026240 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 275.8 bits (704), Expect = 6.9e-74
Identity = 171/465 (36.77%), Postives = 266/465 (57.20%), Query Frame = 0

Query: 2   PPWVVLLIGAVMNFFGYFMIWLGVTRRISTPKVWQMCFYICMGGSSQSFANTGSMVTCVN 61
           P WVVLL+GA  N FGY ++WL VT ++    +W +   I +G + +++ NT S+V+C++
Sbjct: 80  PSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIH 139

Query: 62  NFPERRGVVLGLLKGYIGLSGAIITQLFHAFYGGDTKSLILFIGWLPAAISFAFLRTVRI 121
           NFPE RG V+G+LKG+ GLSGAI+TQ++  F      S+IL +   P  +  A L  VR 
Sbjct: 140 NFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPPVVVLALLFIVRP 199

Query: 122 MKVIRQPNELKVFYNFLYI---SLLLAGFLMLMIIVQSKTEFTQNQYGGSAAAIVVLLLL 181
           ++   + N       FL I    ++LA +L+ ++++QS  + TQ     S A +V+ +++
Sbjct: 200 VERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVV 259

Query: 182 PLAV----VTIEECNLQKLKTKSPNSSVQIITEK--LPKTEHSKQKEPSCWTTIFNPPQR 241
           P+ V    V I   N+  +K +   S+V     +  + +++   +K+          P  
Sbjct: 260 PVLVPFSSVFISGNNVTSVKPEEGTSNVDQHEARTLIERSDRPPEKK--------RAPCI 319

Query: 242 GEDFTILQAVFSVDMLILFISVICGAGGTLTAVDNLGQIGMSLGYPKRSISTFVSLVSIW 301
           GEDFT+LQA+   D  ++F+S++ G G  +T +DNLGQI  SLGY    I  FVSL+SI 
Sbjct: 320 GEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKI--FVSLISIS 379

Query: 302 NYLGRVVSGFVSEIVLIKYKFPRPLMLSLNLLLSCVGYLVIAFDVPNGLYVASIVIGFCV 361
           N+LGRV  G+ SE+++ K   PR L +S+   +  +G +  A D P  +YV +IVIG   
Sbjct: 380 NFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGY 439

Query: 362 GAQWPLIYAIISEIFGLKYYSTLYNFGTVAMPIGLYIMNVKVAGNFYDREAEKQLKAKGI 421
           GA W +  A +S+IFGLK + +LYNF   A+PIG ++ +  +A N YD  A KQ      
Sbjct: 440 GAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQAGP--- 499

Query: 422 IRKAGEDLKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFY 458
                E L C G  C+ ++  +++ + LM M +SL +V RTR FY
Sbjct: 500 -TTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E13.3e-2023.73Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q6CGU82.5e-0426.25Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28... [more]
Match NameE-valueIdentityDescription
XP_031741165.10.0100.00uncharacterized protein LOC101205265 [Cucumis sativus] >KAE8648845.1 hypothetica... [more]
XP_031741159.10.089.31uncharacterized protein LOC101205029 [Cucumis sativus] >KAE8648846.1 hypothetica... [more]
XP_008446819.17.76e-29684.88PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0034695.1 ... [more]
XP_022956604.19.55e-29482.31protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
XP_038893353.15.30e-29382.60LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A5D3CFW33.76e-29684.88Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BG093.76e-29684.88protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 ... [more]
A0A6J1GZI84.62e-29482.31protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K1867.67e-29182.09protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489... [more]
A0A6J1FZF26.17e-27879.76protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326... [more]
Match NameE-valueIdentityDescription
AT2G39210.11.9e-19367.45Major facilitator superfamily protein [more]
AT2G28120.13.4e-15858.09Major facilitator superfamily protein [more]
AT5G14120.17.3e-7634.65Major facilitator superfamily protein [more]
AT1G74780.14.8e-7533.98Nodulin-like / Major Facilitator Superfamily protein [more]
AT5G50630.16.9e-7436.77Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 268..452
e-value: 5.9E-9
score: 35.3
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 240..458
e-value: 4.6E-10
score: 41.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 5..455
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 1..183
e-value: 1.7E-63
score: 214.5
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 2..483
NoneNo IPR availablePANTHERPTHR21576:SF122MFS TRANSPORTERcoord: 2..483

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G026240.2CsGy5G026240.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0099587 inorganic ion import across plasma membrane
biological_process GO:0002239 response to oomycetes
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0022857 transmembrane transporter activity