CsGy5G019860 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G019860
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionEukaryotic aspartyl protease family protein
LocationGy14Chr5: 26089276 .. 26092339 (-)
RNA-Seq ExpressionCsGy5G019860
SyntenyCsGy5G019860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATTTACATCTCTCTTTCTCTCTTTTTGATCTTTTTCAAAATTTACATCTTCAAATGTAAAACCCAAATAAATTCTCTTATTAATTACTTCCATCTTGTCCCTCAAATATAGAACCCAAATCCTCAACTTTCAAAAGGTCCCATTCTGATTTTCTAAACCCCAAAAAATTTTAAACCATGTTCTTTAAACAACTCTTCTCTTCTCTTCTCTTCCTTCTTGTTGTCATCGTCGGCGCCGCGGCTGCCGCTCGTCTGTCTTCTTCAACAAAGTTTCAATACCTTAATGTTAAAGCTACCAAGTTGGACTTCAATGACGGTCAAATTCTACACGCTCTTAATTTCTCCGACGGTCACCGGCAAGTTTCCGGTTATAAATCCGACAATAATACATTTAAACTCAACCTTCTCCATAGGGATAAACTGTCCCATGTCCATGGTCACCGTCGTGGGTTCAACGACCGCATGAAAAGAGACGCCATAAGAGTCGCCACCCTCGTCCGCCGCCTGTCCCACGGCGCCCCCGCCGCCGTGAAAGATAGCCGTTACAAGGTGGCTAATTTTGCCACGGATGTCATTTCAGGCAAGGAAAATTATTTTTAAAAAACACCCTTTAAAATCCAATGTGTTTATTATCTTCTTGAAATGTTTTATATATGATTAAATTGGATTTGGAAATAGGAATGGAAGCGGGGAGTGGAGAATACTTTGTTCGGATCGGAGTTGGAAGCCCGCCGAGGAATCAATATATGGTGATTGATTCCGGCAGTGATATTGTGTGGGTTCAATGTAAACCTTGTAGCCGATGCTACCAACAATCCGACCCGGTTTTCGATCCGGCTGACTCCTCCTCCTTCGCCGGCGTCTCTTGTGGCTCCGACGTTTGCGACCGCCTTGAGAACACCGGATGTAACGCCGGACGATGTCGGTATGAAGTGTCTTATGGGGACGGGTCTTACACTAAAGGCACTCTCGCTCTCGAAACTCTCACCGTCGGCCAAGTAAGCAATTTAACTCAAAAACTTAGCTTAAAACTTAAATATTAGAGCATTAAGTTAATTAATGTGGTAGTTTAAGCATATTGATATTAATAATAATGTCTCACAAGTTTGGTATATGATAGAGTGTTTAGAGCATGTAGGTTGTGATATAGTGTATGATTATGTAAGTTAATTATTATAATTAGAACTCTAACTATAAAGTAACAGTCACACACCTCTTTGATTTTTAAAGGTGATAGATATTTAAGTTTGTAAGTGAAGAAATAAGTATATTCATTAATTTTGTGTGGAAGAGATGAGTAGAAACTTTAAAAAAGAAATAGTATGTGTGTTATGCACTTCAAATTAAGGAAGGAGTATTTAAAATATTTTTTGTAAAGTTAGAAACCTTTAAAATGCTTTTTTCCTTTTTTAAAAAAGGAAAATTAAAGGTCTAATTTAATCTTTTTATTCTTTTTCTAAAAAATTTTTCATGTGACGCAATCATCCAACCACTACAAAGAAGGAAAAAATGATACATAAAGTTTTCACGTGAAGAAGTTCAAATTTAAGTTTGTGTTTAAAGATCATTTAAGTAAATAATTTTGTTTTTAAAAAATAAATTAACTTTAAATTTTGTCTATAAATTCTAATCTATTAATTTTGTAATTGATAGATATATTTTGGGTAAGTCACTGATTTTTCATTAGATTTTTGTGTTTAATATATCAATAAATCTATAATATATTTGAATTTTCTTTCAAACTAGTTCTATATGAAAATAGAAAATAAGATTTATGGTTTCAAAGTTGTATGAACTTTGCAATTTAATTTTTAATAGATATGAGTTTTAGAAAATGTAAATTATCTATTGGATACAAATGCGAAAATTGAAGGTAATGGTAATATTTAGTTTTTAAAAATTAAGCTTTAGAATATTTCTTCAAATTTTGAAAACTTAAAATGCATTTTTTAAAGTAGAATTTGGCTAACTATTTTACTAGAATTTAGACTCAAGTCGAATTGAACACTAACTAAATCGTCTTAATCGGCTAGAGGACAATCTTATTGGAAAATTGGTTTCGAGTAATCGATGCTCAGCTCAAAGTGAGTTAAGTTTAAAAAGTTTAATCCTTAATTTTTGGTATTTTTTAATCTGATTAGGTGATGATTCGTGATGTGGCAATTGGGTGTGGTCATACAAACCAAGGAATGTTCATAGGTGCCGCGGGTTTACTCGGACTCGGTGGAGGGTCAATGTCCTTCATCGGCCAACTAGGTGGTCAAACCGGGGGAGCATTCAGCTATTGTTTAGTGAGCCGAGGCACTGGCTCAACCGGAGCGTTAGAATTCGGCCGTGGAGCATTGCCAGTTGGCGCCACATGGATCTCCCTAATTCGAAACCCACGCGCTCCAAGCTTCTACTACATCGGACTCGCCGGGATCGGTGTTGGCGGTGTTCGAGTTTCAGTACCAGAAGAAACGTTTCAACTCACTGAGTACGGTACCAACGGTGTTGTAATGGACACTGGTACCGCCGTGACACGTTTTCCGACTGCGGCCTACGTAGCGTTCCGTGATTCGTTCACAGCCCAAACCAGCAACCTCCCACGAGCGCCTGGCGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTTGAGTCAGTGCGAGTGCCCACGGTGTCGTTTTACTTCTCCGATGGACCGGTTCTGACATTGCCAGCGAGGAACTTTTTGATTCCAGTAGACGGCGGCGGAACGTTTTGCTTGGCGTTTGCTCCGTCGCCGTCAGGGCTTTCTATAATCGGAAACATCCAGCAAGAAGGAATTCAAATTTCATTCGATGGGGCTAATGGGTTCGTTGGATTTGGCCCAAATATTTGCTAAATCTGCAAATTATATAATTAAGGAATTTGGAATATAGTTTTTGTTAATCACATTATTAATTTTTGTTTTAAAGCTAATAGATAGATCACATGTAAATTATTTACTTCCCATTTAGTACAGCTATATAGTTTCTATGAGTGTACTATTTTTCTTATTTCCAAATGTAATAAAATCTATTCTACTTCCAATCTATAAA

mRNA sequence

ATATTTACATCTCTCTTTCTCTCTTTTTGATCTTTTTCAAAATTTACATCTTCAAATGTAAAACCCAAATAAATTCTCTTATTAATTACTTCCATCTTGTCCCTCAAATATAGAACCCAAATCCTCAACTTTCAAAAGGTCCCATTCTGATTTTCTAAACCCCAAAAAATTTTAAACCATGTTCTTTAAACAACTCTTCTCTTCTCTTCTCTTCCTTCTTGTTGTCATCGTCGGCGCCGCGGCTGCCGCTCGTCTGTCTTCTTCAACAAAGTTTCAATACCTTAATGTTAAAGCTACCAAGTTGGACTTCAATGACGGTCAAATTCTACACGCTCTTAATTTCTCCGACGGTCACCGGCAAGTTTCCGGTTATAAATCCGACAATAATACATTTAAACTCAACCTTCTCCATAGGGATAAACTGTCCCATGTCCATGGTCACCGTCGTGGGTTCAACGACCGCATGAAAAGAGACGCCATAAGAGTCGCCACCCTCGTCCGCCGCCTGTCCCACGGCGCCCCCGCCGCCGTGAAAGATAGCCGTTACAAGGTGGCTAATTTTGCCACGGATGTCATTTCAGGAATGGAAGCGGGGAGTGGAGAATACTTTGTTCGGATCGGAGTTGGAAGCCCGCCGAGGAATCAATATATGGTGATTGATTCCGGCAGTGATATTGTGTGGGTTCAATGTAAACCTTGTAGCCGATGCTACCAACAATCCGACCCGGTTTTCGATCCGGCTGACTCCTCCTCCTTCGCCGGCGTCTCTTGTGGCTCCGACGTTTGCGACCGCCTTGAGAACACCGGATGTAACGCCGGACGATGTCGGTATGAAGTGTCTTATGGGGACGGGTCTTACACTAAAGGCACTCTCGCTCTCGAAACTCTCACCGTCGGCCAAGTGATGATTCGTGATGTGGCAATTGGGTGTGGTCATACAAACCAAGGAATGTTCATAGGTGCCGCGGGTTTACTCGGACTCGGTGGAGGGTCAATGTCCTTCATCGGCCAACTAGGTGGTCAAACCGGGGGAGCATTCAGCTATTGTTTAGTGAGCCGAGGCACTGGCTCAACCGGAGCGTTAGAATTCGGCCGTGGAGCATTGCCAGTTGGCGCCACATGGATCTCCCTAATTCGAAACCCACGCGCTCCAAGCTTCTACTACATCGGACTCGCCGGGATCGGTGTTGGCGGTGTTCGAGTTTCAGTACCAGAAGAAACGTTTCAACTCACTGAGTACGGTACCAACGGTGTTGTAATGGACACTGGTACCGCCGTGACACGTTTTCCGACTGCGGCCTACGTAGCGTTCCGTGATTCGTTCACAGCCCAAACCAGCAACCTCCCACGAGCGCCTGGCGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTTGAGTCAGTGCGAGTGCCCACGGTGTCGTTTTACTTCTCCGATGGACCGGTTCTGACATTGCCAGCGAGGAACTTTTTGATTCCAGTAGACGGCGGCGGAACGTTTTGCTTGGCGTTTGCTCCGTCGCCGTCAGGGCTTTCTATAATCGGAAACATCCAGCAAGAAGGAATTCAAATTTCATTCGATGGGGCTAATGGGTTCGTTGGATTTGGCCCAAATATTTGCTAAATCTGCAAATTATATAATTAAGGAATTTGGAATATAGTTTTTGTTAATCACATTATTAATTTTTGTTTTAAAGCTAATAGATAGATCACATGTAAATTATTTACTTCCCATTTAGTACAGCTATATAGTTTCTATGAGTGTACTATTTTTCTTATTTCCAAATGTAATAAAATCTATTCTACTTCCAATCTATAAA

Coding sequence (CDS)

ATGTTCTTTAAACAACTCTTCTCTTCTCTTCTCTTCCTTCTTGTTGTCATCGTCGGCGCCGCGGCTGCCGCTCGTCTGTCTTCTTCAACAAAGTTTCAATACCTTAATGTTAAAGCTACCAAGTTGGACTTCAATGACGGTCAAATTCTACACGCTCTTAATTTCTCCGACGGTCACCGGCAAGTTTCCGGTTATAAATCCGACAATAATACATTTAAACTCAACCTTCTCCATAGGGATAAACTGTCCCATGTCCATGGTCACCGTCGTGGGTTCAACGACCGCATGAAAAGAGACGCCATAAGAGTCGCCACCCTCGTCCGCCGCCTGTCCCACGGCGCCCCCGCCGCCGTGAAAGATAGCCGTTACAAGGTGGCTAATTTTGCCACGGATGTCATTTCAGGAATGGAAGCGGGGAGTGGAGAATACTTTGTTCGGATCGGAGTTGGAAGCCCGCCGAGGAATCAATATATGGTGATTGATTCCGGCAGTGATATTGTGTGGGTTCAATGTAAACCTTGTAGCCGATGCTACCAACAATCCGACCCGGTTTTCGATCCGGCTGACTCCTCCTCCTTCGCCGGCGTCTCTTGTGGCTCCGACGTTTGCGACCGCCTTGAGAACACCGGATGTAACGCCGGACGATGTCGGTATGAAGTGTCTTATGGGGACGGGTCTTACACTAAAGGCACTCTCGCTCTCGAAACTCTCACCGTCGGCCAAGTGATGATTCGTGATGTGGCAATTGGGTGTGGTCATACAAACCAAGGAATGTTCATAGGTGCCGCGGGTTTACTCGGACTCGGTGGAGGGTCAATGTCCTTCATCGGCCAACTAGGTGGTCAAACCGGGGGAGCATTCAGCTATTGTTTAGTGAGCCGAGGCACTGGCTCAACCGGAGCGTTAGAATTCGGCCGTGGAGCATTGCCAGTTGGCGCCACATGGATCTCCCTAATTCGAAACCCACGCGCTCCAAGCTTCTACTACATCGGACTCGCCGGGATCGGTGTTGGCGGTGTTCGAGTTTCAGTACCAGAAGAAACGTTTCAACTCACTGAGTACGGTACCAACGGTGTTGTAATGGACACTGGTACCGCCGTGACACGTTTTCCGACTGCGGCCTACGTAGCGTTCCGTGATTCGTTCACAGCCCAAACCAGCAACCTCCCACGAGCGCCTGGCGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTTGAGTCAGTGCGAGTGCCCACGGTGTCGTTTTACTTCTCCGATGGACCGGTTCTGACATTGCCAGCGAGGAACTTTTTGATTCCAGTAGACGGCGGCGGAACGTTTTGCTTGGCGTTTGCTCCGTCGCCGTCAGGGCTTTCTATAATCGGAAACATCCAGCAAGAAGGAATTCAAATTTCATTCGATGGGGCTAATGGGTTCGTTGGATTTGGCCCAAATATTTGCTAA

Protein sequence

MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHRQVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKDSRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC*
Homology
BLAST of CsGy5G019860 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 4.7e-181
Identity = 311/450 (69.11%), Postives = 365/450 (81.11%), Query Frame = 0

Query: 35  LNVKATKLDFNDGQILHALNFSDGHRQVSGYKSDNNTFKLNLLHRDKLSHV--HGHRRGF 94
           L V AT  DFN+       +FSD           ++ + L LLHRD+   V    H    
Sbjct: 37  LTVTATLPDFNN------THFSD---------ESSSKYTLRLLHRDRFPSVTYRNHHHRL 96

Query: 95  NDRMKRDAIRVATLVRRLSHGAPAAVKDSRYKVANFATDVISGMEAGSGEYFVRIGVGSP 154
           + RM+RD  RV+ ++RR+S G      DSRY+V +F +D++SGM+ GSGEYFVRIGVGSP
Sbjct: 97  HARMRRDTDRVSAILRRIS-GKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSP 156

Query: 155 PRNQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCN 214
           PR+QYMVIDSGSD+VWVQC+PC  CY+QSDPVFDPA S S+ GVSCGS VCDR+EN+GC+
Sbjct: 157 PRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCH 216

Query: 215 AGRCRYEVSYGDGSYTKGTLALETLTVGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGS 274
           +G CRYEV YGDGSYTKGTLALETLT  + ++R+VA+GCGH N+GMFIGAAGLLG+GGGS
Sbjct: 217 SGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGS 276

Query: 275 MSFIGQLGGQTGGAFSYCLVSRGTGSTGALEFGRGALPVGATWISLIRNPRAPSFYYIGL 334
           MSF+GQL GQTGGAF YCLVSRGT STG+L FGR ALPVGA+W+ L+RNPRAPSFYY+GL
Sbjct: 277 MSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGL 336

Query: 335 AGIGVGGVRVSVPEETFQLTEYGTNGVVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRA 394
            G+GVGGVR+ +P+  F LTE G  GVVMDTGTAVTR PTAAYVAFRD F +QT+NLPRA
Sbjct: 337 KGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRA 396

Query: 395 PGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSG 454
            GVSIFDTCYDL+GF SVRVPTVSFYF++GPVLTLPARNFL+PVD  GT+C AFA SP+G
Sbjct: 397 SGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTG 456

Query: 455 LSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
           LSIIGNIQQEGIQ+SFDGANGFVGFGPN+C
Sbjct: 457 LSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of CsGy5G019860 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 1.3e-106
Identity = 189/399 (47.37%), Postives = 267/399 (66.92%), Query Frame = 0

Query: 95  RMKRDAIRVATLVRRL---------SHGAPAAVKDSRYKVANFATDVISGMEAGSGEYFV 154
           R++RD+ RVA +V ++         S   P   +D+RY+  +  T V+SG   GSGEYF 
Sbjct: 105 RLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFS 164

Query: 155 RIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSDVCDR 214
           RIGVG+P +  Y+V+D+GSD+ W+QC+PC+ CYQQSDPVF+P  SS++  ++C +  C  
Sbjct: 165 RIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSL 224

Query: 215 LENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQV-MIRDVAIGCGHTNQGMFIGAAG 274
           LE + C + +C Y+VSYGDGS+T G LA +T+T G    I +VA+GCGH N+G+F GAAG
Sbjct: 225 LETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAG 284

Query: 275 LLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGALEFGRGALPVGATWISLIRNPRA 334
           LLGLGGG +S   Q+      +FSYCLV R +G + +L+F    L  G     L+RN + 
Sbjct: 285 LLGLGGGVLSITNQM---KATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKI 344

Query: 335 PSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVVMDTGTAVTRFPTAAYVAFRDSFTA 394
            +FYY+GL+G  VGG +V +P+  F +   G+ GV++D GTAVTR  T AY + RD+F  
Sbjct: 345 DTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLK 404

Query: 395 QTSNLPR-APGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPARNFLIPVDGGGTFC 454
            T NL + +  +S+FDTCYD +   +V+VPTV+F+F+ G  L LPA+N+LIPVD  GTFC
Sbjct: 405 LTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFC 464

Query: 455 LAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
            AFAP+ S LSIIGN+QQ+G +I++D +   +G   N C
Sbjct: 465 FAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of CsGy5G019860 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.4e-105
Identity = 204/425 (48.00%), Postives = 273/425 (64.24%), Query Frame = 0

Query: 63  SGYKSD-NNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKDS 122
           SG  S+ +++  LNL H D LS        F+ R++RD+ RV + +  L+   P      
Sbjct: 62  SGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKS-IATLAAQIPGRNVTH 121

Query: 123 RYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQS 182
             +   F++ V+SG+  GSGEYF R+GVG+P R  YMV+D+GSDIVW+QC PC RCY QS
Sbjct: 122 APRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQS 181

Query: 183 DPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGR--CRYEVSYGDGSYTKGTLALETLTV 242
           DP+FDP  S ++A + C S  C RL++ GCN  R  C Y+VSYGDGS+T G  + ETLT 
Sbjct: 182 DPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF 241

Query: 243 GQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGS- 302
            +  ++ VA+GCGH N+G+F+GAAGLLGLG G +SF GQ G +    FSYCLV R   S 
Sbjct: 242 RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSK 301

Query: 303 TGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRV-SVPEETFQLTEYGTN 362
             ++ FG  A+   A +  L+ NP+  +FYY+GL GI VGG RV  V    F+L + G  
Sbjct: 302 PSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNG 361

Query: 363 GVVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 422
           GV++D+GT+VTR    AY+A RD+F      L RAP  S+FDTC+DL+    V+VPTV  
Sbjct: 362 GVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVL 421

Query: 423 YFSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 482
           +F  G  ++LPA N+LIPVD  G FC AFA +  GLSIIGNIQQ+G ++ +D A+  VGF
Sbjct: 422 HFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 481

BLAST of CsGy5G019860 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 6.3e-69
Identity = 154/425 (36.24%), Postives = 224/425 (52.71%), Query Frame = 0

Query: 62  VSGYKSDNNTFKLNLLH-RDKLSHVHGHRRGFNDR-MKRDAIRVATLVRRLSHGAPAAVK 121
           V   K+ N    L ++H     SH+    R  +D  ++RD  RV ++  +LS  +   V 
Sbjct: 53  VPSSKASNTKSSLRVVHMHGACSHLSSDARVDHDEIIRRDQARVESIYSKLSKNSANEVS 112

Query: 122 DSRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPC-SRCY 181
           +++    +      SG+  GSG Y V IG+G+P  +  +V D+GSD+ W QC+PC   CY
Sbjct: 113 EAK----STELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCY 172

Query: 182 QQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLT 241
            Q +P F+P+ SS++  VSC S +C+  E+  C+A  C Y + YGD S+T+G LA E  T
Sbjct: 173 SQKEPKFNPSSSSTYQNVSCSSPMCEDAES--CSASNCVYSIVYGDKSFTQGFLAKEKFT 232

Query: 242 V-GQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 301
           +    ++ DV  GCG  NQG+F G AGLLGLG G +S   Q        FSYCL S  + 
Sbjct: 233 LTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSN 292

Query: 302 STGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTN 361
           STG L FG   +     +  +   P A + Y I + GI VG   +++   +F      T 
Sbjct: 293 STGHLTFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITPNSF-----STE 352

Query: 362 GVVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 421
           G ++D+GT  TR PT  Y   R  F  + S+     G  +FDTCYD  G ++V  PT++F
Sbjct: 353 GAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAF 412

Query: 422 YFSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 481
            F+   V+ L      +P+      CLAFA +    +I GN+QQ  + + +D A G VGF
Sbjct: 413 SFAGSTVVELDGSGISLPIK-ISQVCLAFAGNDDLPAIFGNVQQTTLDVVYDVAGGRVGF 464

Query: 482 GPNIC 483
            PN C
Sbjct: 473 APNGC 464

BLAST of CsGy5G019860 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-68
Identity = 145/357 (40.62%), Postives = 200/357 (56.02%), Query Frame = 0

Query: 135 GMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSR-CYQQSDPVFDPADSSSF 194
           G   GSG Y V +G+G+P  +  ++ D+GSD+ W QC+PC R CY Q +P+F+P+ S+S+
Sbjct: 124 GSTLGSGNYIVTVGLGTPKNDLSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSY 183

Query: 195 AGVSCGSDVCDRLENT-----GCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVMIRD-V 254
             VSC S  C  L +       C+A  C Y + YGD S++ G LA E  T+    + D V
Sbjct: 184 YNVSCSSAACGSLSSATGNAGSCSASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGV 243

Query: 255 AIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGALEFGRG 314
             GCG  NQG+F G AGLLGLG   +SF  Q        FSYCL S     TG L FG  
Sbjct: 244 YFGCGENNQGLFTGVAGLLGLGRDKLSFPSQTATAYNKIFSYCLPS-SASYTGHLTFGSA 303

Query: 315 ALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVVMDTGTAV 374
            +     +  +       SFY + +  I VGG ++ +P      T + T G ++D+GT +
Sbjct: 304 GISRSVKFTPISTITDGTSFYGLNIVAITVGGQKLPIPS-----TVFSTPGALIDSGTVI 363

Query: 375 TRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTL 434
           TR P  AY A R SF A+ S  P   GVSI DTC+DL+GF++V +P V+F FS G V+ L
Sbjct: 364 TRLPPKAYAALRSSFKAKMSKYPTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVEL 423

Query: 435 PARNFLIPVDGGGTFCLAFA--PSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
            ++  +  V      CLAFA     S  +I GN+QQ+ +++ +DGA G VGF PN C
Sbjct: 424 GSKG-IFYVFKISQVCLAFAGNSDDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

BLAST of CsGy5G019860 vs. NCBI nr
Match: XP_004147103.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KGN51568.1 hypothetical protein Csa_008597 [Cucumis sativus])

HSP 1 Score: 962 bits (2486), Expect = 0.0
Identity = 482/482 (100.00%), Postives = 482/482 (100.00%), Query Frame = 0

Query: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60
           MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR
Sbjct: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60

Query: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120
           QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD
Sbjct: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120

Query: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180
           SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ
Sbjct: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180

Query: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240
           SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG
Sbjct: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240

Query: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300
           QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG
Sbjct: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300

Query: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360
           ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV
Sbjct: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480
           DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480

Query: 481 IC 482
           IC
Sbjct: 481 IC 482

BLAST of CsGy5G019860 vs. NCBI nr
Match: XP_008445900.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo])

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 456/482 (94.61%), Postives = 466/482 (96.68%), Query Frame = 0

Query: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60
           MFFKQL SSLLF L V+V  AAAAR SS TKFQYLNVKATKLDFNDGQILH LNFSD HR
Sbjct: 1   MFFKQLISSLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHR 60

Query: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120
           QVSG+KS N+TFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA AAV+D
Sbjct: 61  QVSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA-AAVED 120

Query: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180
           SR+KVANFATDVISGME GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQCKPCSRCYQQ
Sbjct: 121 SRFKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQ 180

Query: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240
           SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG
Sbjct: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240

Query: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300
           QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG
Sbjct: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300

Query: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360
            LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVS+PEETFQLTE+GTNGVV
Sbjct: 301 TLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVV 360

Query: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTR PT+AYVA RDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480
           DGP LTLPA+NFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 421 DGPALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480

Query: 481 IC 482
           IC
Sbjct: 481 IC 481

BLAST of CsGy5G019860 vs. NCBI nr
Match: KAA0034086.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 876 bits (2264), Expect = 0.0
Identity = 443/482 (91.91%), Postives = 453/482 (93.98%), Query Frame = 0

Query: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60
           MFFKQL SSLLF L V+V  AAAAR SS TKFQYLNVKATKLDFNDGQILH LNFSD HR
Sbjct: 1   MFFKQLISSLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHR 60

Query: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120
           QVSG+KS N+TFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA AAV+D
Sbjct: 61  QVSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA-AAVED 120

Query: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180
           SR+K              GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQCKPCSRCYQQ
Sbjct: 121 SRFK--------------GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQ 180

Query: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240
           SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG
Sbjct: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240

Query: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300
           QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG
Sbjct: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300

Query: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360
            LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVS+PEETFQLTE+GTNGVV
Sbjct: 301 TLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVV 360

Query: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTR PT+AYVA RDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480
           DGP LTLPA+NFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 421 DGPALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 467

Query: 481 IC 482
           IC
Sbjct: 481 IC 467

BLAST of CsGy5G019860 vs. NCBI nr
Match: XP_038893071.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 443/482 (91.91%), Postives = 456/482 (94.61%), Query Frame = 0

Query: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60
           MF KQ  SSLLF L  +  +AAA R SS TKFQYLNVKATKLDFND QILH LNFS   R
Sbjct: 1   MFLKQP-SSLLFFLFFLFFSAAAGR-SSPTKFQYLNVKATKLDFNDDQILHTLNFSHARR 60

Query: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120
           QVSG+KSDNNTFKLNLLHRDKLSHVHGHR GFN+R+KRDAIRVATLVRRLSHG   AV+D
Sbjct: 61  QVSGHKSDNNTFKLNLLHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGG-GAVQD 120

Query: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180
           ++YKVA+FATDVISGME GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQCKPCSRCYQQ
Sbjct: 121 NKYKVADFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQ 180

Query: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240
           SDPVFDPADSSSFAGVSC S VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG
Sbjct: 181 SDPVFDPADSSSFAGVSCDSIVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240

Query: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300
           +VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG
Sbjct: 241 EVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300

Query: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360
            LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV
Sbjct: 301 TLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTR PTAAYVA RDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480
           DGPVLTLPA+NFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 479

Query: 481 IC 482
           +C
Sbjct: 481 VC 479

BLAST of CsGy5G019860 vs. NCBI nr
Match: XP_023547721.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 803 bits (2074), Expect = 1.67e-290
Identity = 396/473 (83.72%), Postives = 428/473 (90.49%), Query Frame = 0

Query: 10  LLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHRQVSGYKSDN 69
           LL LL+ +  AAA AR S    F YLNVK TKL+ N   IL  LNFSD H QV+  K  N
Sbjct: 13  LLHLLLFLAAAAALARPSPPPNFLYLNVKPTKLELNHTHILPTLNFSDAHLQVTAQKFHN 72

Query: 70  NTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKDSRYKVANFA 129
            T KLNLLHRDKL HVH HR  FN+R+KRDAIRVATL+RRLSH A AA +D+ YKVANFA
Sbjct: 73  TTLKLNLLHRDKLPHVHAHRHAFNERIKRDAIRVATLIRRLSHAAAAAAQDNSYKVANFA 132

Query: 130 TDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPAD 189
           TDV+SGME GSGE+FVR+GVGSPPR+QYMVIDSGSDIVWVQC+PC++CYQQSDPVF+PAD
Sbjct: 133 TDVVSGMEEGSGEFFVRVGVGSPPRSQYMVIDSGSDIVWVQCQPCTQCYQQSDPVFNPAD 192

Query: 190 SSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVMIRDVAI 249
           SSSFAGVSCGS VCDRL+NT C+AGRCRYEVSYGDGSYTKGTLALET+T+GQVMIRDVAI
Sbjct: 193 SSSFAGVSCGSAVCDRLDNTDCHAGRCRYEVSYGDGSYTKGTLALETITMGQVMIRDVAI 252

Query: 250 GCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGALEFGRGAL 309
           GCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSR TGSTG LEFGRGA+
Sbjct: 253 GCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRDTGSTGTLEFGRGAM 312

Query: 310 PVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVVMDTGTAVTR 369
           PVGATWISLIRNPRAPSFYYIGLAG+GVGGVRV +PEETFQL+EYGTNGVVMDTGTAVTR
Sbjct: 313 PVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPIPEETFQLSEYGTNGVVMDTGTAVTR 372

Query: 370 FPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPA 429
            PTAAY AFRD+FTAQT+NLPRA GVSIFDTC+DLNGFES+RVPTVSFYFSDGPVLTLPA
Sbjct: 373 LPTAAYEAFRDAFTAQTNNLPRAGGVSIFDTCFDLNGFESIRVPTVSFYFSDGPVLTLPA 432

Query: 430 RNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 482
           +NFLIPV+G GTFCLAFAPSPSGLSIIGNIQQEGIQIS DGANGFVGFGPN+C
Sbjct: 433 KNFLIPVNGAGTFCLAFAPSPSGLSIIGNIQQEGIQISIDGANGFVGFGPNVC 485

BLAST of CsGy5G019860 vs. ExPASy TrEMBL
Match: A0A0A0KPR4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579060 PE=4 SV=1)

HSP 1 Score: 962 bits (2486), Expect = 0.0
Identity = 482/482 (100.00%), Postives = 482/482 (100.00%), Query Frame = 0

Query: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60
           MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR
Sbjct: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60

Query: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120
           QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD
Sbjct: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120

Query: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180
           SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ
Sbjct: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180

Query: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240
           SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG
Sbjct: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240

Query: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300
           QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG
Sbjct: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300

Query: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360
           ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV
Sbjct: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480
           DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480

Query: 481 IC 482
           IC
Sbjct: 481 IC 482

BLAST of CsGy5G019860 vs. ExPASy TrEMBL
Match: A0A1S3BEG7 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103488783 PE=4 SV=1)

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 456/482 (94.61%), Postives = 466/482 (96.68%), Query Frame = 0

Query: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60
           MFFKQL SSLLF L V+V  AAAAR SS TKFQYLNVKATKLDFNDGQILH LNFSD HR
Sbjct: 1   MFFKQLISSLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHR 60

Query: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120
           QVSG+KS N+TFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA AAV+D
Sbjct: 61  QVSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA-AAVED 120

Query: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180
           SR+KVANFATDVISGME GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQCKPCSRCYQQ
Sbjct: 121 SRFKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQ 180

Query: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240
           SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG
Sbjct: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240

Query: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300
           QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG
Sbjct: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300

Query: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360
            LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVS+PEETFQLTE+GTNGVV
Sbjct: 301 TLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVV 360

Query: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTR PT+AYVA RDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480
           DGP LTLPA+NFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 421 DGPALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480

Query: 481 IC 482
           IC
Sbjct: 481 IC 481

BLAST of CsGy5G019860 vs. ExPASy TrEMBL
Match: A0A5A7SVY2 (Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00740 PE=4 SV=1)

HSP 1 Score: 876 bits (2264), Expect = 0.0
Identity = 443/482 (91.91%), Postives = 453/482 (93.98%), Query Frame = 0

Query: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60
           MFFKQL SSLLF L V+V  AAAAR SS TKFQYLNVKATKLDFNDGQILH LNFSD HR
Sbjct: 1   MFFKQLISSLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHR 60

Query: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120
           QVSG+KS N+TFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA AAV+D
Sbjct: 61  QVSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGA-AAVED 120

Query: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180
           SR+K              GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQCKPCSRCYQQ
Sbjct: 121 SRFK--------------GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQ 180

Query: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240
           SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG
Sbjct: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240

Query: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300
           QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG
Sbjct: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300

Query: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360
            LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVS+PEETFQLTE+GTNGVV
Sbjct: 301 TLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVV 360

Query: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTR PT+AYVA RDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480
           DGP LTLPA+NFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 421 DGPALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 467

Query: 481 IC 482
           IC
Sbjct: 481 IC 467

BLAST of CsGy5G019860 vs. ExPASy TrEMBL
Match: A0A6J1KA06 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493608 PE=4 SV=1)

HSP 1 Score: 790 bits (2040), Expect = 1.26e-285
Identity = 401/487 (82.34%), Postives = 434/487 (89.12%), Query Frame = 0

Query: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSST---KFQYLNVKATKLDFNDGQILHALNFSD 60
           MF K   S LL LL ++  AAAAA L+  +    F YLNVK TKL+ N  QIL  LNFSD
Sbjct: 1   MFLKHPSSPLLLLLHLLFLAAAAAALARPSLPPNFLYLNVKPTKLELNHTQILPTLNFSD 60

Query: 61  GHRQVSGYKSDNNTFKLNLLHRDKLSHVHGHRR--GFNDRMKRDAIRVATLVRRLSHGAP 120
            H QV+  K  N T KLNLLHRDKL HVH HR    FN+R+KRDAIRVATLVRRLSH A 
Sbjct: 61  AHLQVTAQKFHNTTLKLNLLHRDKLPHVHAHRHRHAFNERIKRDAIRVATLVRRLSHTA- 120

Query: 121 AAVKDSRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCS 180
           AAV+D+ YKVANFAT V+SGME GSGE+FVR+GVGSPPR+QYMVIDSGSDIVWVQC+PC+
Sbjct: 121 AAVQDNSYKVANFATGVVSGMEEGSGEFFVRVGVGSPPRSQYMVIDSGSDIVWVQCQPCT 180

Query: 181 RCYQQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALE 240
           +CYQQSDPVF+PADSSSFAGVSCGS VC RL+NT C+AGRCRYEVSYGDGSYTKGTLALE
Sbjct: 181 QCYQQSDPVFNPADSSSFAGVSCGSAVCGRLDNTDCHAGRCRYEVSYGDGSYTKGTLALE 240

Query: 241 TLTVGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRG 300
           T+T+GQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSR 
Sbjct: 241 TITMGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRD 300

Query: 301 TGSTGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYG 360
           TGSTG LEFGRGA+PVGATWISLIRNPRAPSFYYIGLAG+GVGGVRV +PEETFQL+EYG
Sbjct: 301 TGSTGTLEFGRGAMPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPIPEETFQLSEYG 360

Query: 361 TNGVVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTV 420
           TNGVVMDTGTAVTR PTAAY AFRD+FTAQT+NLPRA GVSIFDTC+DLNGFES+RVPTV
Sbjct: 361 TNGVVMDTGTAVTRLPTAAYEAFRDAFTAQTNNLPRAGGVSIFDTCFDLNGFESIRVPTV 420

Query: 421 SFYFSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFV 480
           SFYFSDGPVLTLPA+NFLIPV+G GTFCLAFAPSPSGLSIIGNIQQEGIQIS DGANGFV
Sbjct: 421 SFYFSDGPVLTLPAKNFLIPVNGAGTFCLAFAPSPSGLSIIGNIQQEGIQISIDGANGFV 480

Query: 481 GFGPNIC 482
           GFGPN+C
Sbjct: 481 GFGPNVC 486

BLAST of CsGy5G019860 vs. ExPASy TrEMBL
Match: A0A6J1G1D0 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=LOC111449773 PE=4 SV=1)

HSP 1 Score: 781 bits (2018), Expect = 2.26e-282
Identity = 388/484 (80.17%), Postives = 427/484 (88.22%), Query Frame = 0

Query: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60
           M  K  FS LLF  V +V  +     SS  +FQYLNVK++K D ND QILH LNFS+GHR
Sbjct: 1   MLLKLHFSLLLFFSVSVVFGSR----SSPVEFQYLNVKSSKFDANDSQILHILNFSNGHR 60

Query: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSH--GAPAAV 120
            V+G KS++  FKL L+HRD+L H+HG  +GF++R+KRD  RVATLVRRLS      + V
Sbjct: 61  LVTGRKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGV 120

Query: 121 KDSRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCY 180
           +D+ YKVANF T VISGME G+GEYFVRIGVGSPPR+QYMVIDSGSDIVWVQC+PC +CY
Sbjct: 121 QDNNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCY 180

Query: 181 QQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLT 240
           QQSDPVF+PA+S+S+ GVSCGS VCDRLEN GC+AGRCRYEVSYGDGSYTKGTLALETLT
Sbjct: 181 QQSDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLT 240

Query: 241 VGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGS 300
           +G+VMI D+AIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRG GS
Sbjct: 241 IGEVMIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGS 300

Query: 301 TGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNG 360
           TG LEFGRGA+PVGATWISL+RNP APSFYYIGLAG+GVGG++V +PEETFQLTEYGTNG
Sbjct: 301 TGTLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNG 360

Query: 361 VVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFY 420
           VVMDTGTAVTR PTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCY+LNGFESVRVPTVSFY
Sbjct: 361 VVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFY 420

Query: 421 FSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFG 480
           FSDGPVLTLPA NFLIPVD  GTFC AFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFG
Sbjct: 421 FSDGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFG 480

Query: 481 PNIC 482
           PNIC
Sbjct: 481 PNIC 480

BLAST of CsGy5G019860 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 635.6 bits (1638), Expect = 3.3e-182
Identity = 311/450 (69.11%), Postives = 365/450 (81.11%), Query Frame = 0

Query: 35  LNVKATKLDFNDGQILHALNFSDGHRQVSGYKSDNNTFKLNLLHRDKLSHV--HGHRRGF 94
           L V AT  DFN+       +FSD           ++ + L LLHRD+   V    H    
Sbjct: 37  LTVTATLPDFNN------THFSD---------ESSSKYTLRLLHRDRFPSVTYRNHHHRL 96

Query: 95  NDRMKRDAIRVATLVRRLSHGAPAAVKDSRYKVANFATDVISGMEAGSGEYFVRIGVGSP 154
           + RM+RD  RV+ ++RR+S G      DSRY+V +F +D++SGM+ GSGEYFVRIGVGSP
Sbjct: 97  HARMRRDTDRVSAILRRIS-GKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSP 156

Query: 155 PRNQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCN 214
           PR+QYMVIDSGSD+VWVQC+PC  CY+QSDPVFDPA S S+ GVSCGS VCDR+EN+GC+
Sbjct: 157 PRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCH 216

Query: 215 AGRCRYEVSYGDGSYTKGTLALETLTVGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGS 274
           +G CRYEV YGDGSYTKGTLALETLT  + ++R+VA+GCGH N+GMFIGAAGLLG+GGGS
Sbjct: 217 SGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGS 276

Query: 275 MSFIGQLGGQTGGAFSYCLVSRGTGSTGALEFGRGALPVGATWISLIRNPRAPSFYYIGL 334
           MSF+GQL GQTGGAF YCLVSRGT STG+L FGR ALPVGA+W+ L+RNPRAPSFYY+GL
Sbjct: 277 MSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGL 336

Query: 335 AGIGVGGVRVSVPEETFQLTEYGTNGVVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRA 394
            G+GVGGVR+ +P+  F LTE G  GVVMDTGTAVTR PTAAYVAFRD F +QT+NLPRA
Sbjct: 337 KGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRA 396

Query: 395 PGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSG 454
            GVSIFDTCYDL+GF SVRVPTVSFYF++GPVLTLPARNFL+PVD  GT+C AFA SP+G
Sbjct: 397 SGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTG 456

Query: 455 LSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
           LSIIGNIQQEGIQ+SFDGANGFVGFGPN+C
Sbjct: 457 LSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of CsGy5G019860 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 392.1 bits (1006), Expect = 6.4e-109
Identity = 189/396 (47.73%), Postives = 265/396 (66.92%), Query Frame = 0

Query: 95  RMKRDAIRVATLVRRLSHGAPAAVKDSRYKVANFAT--------DVISGMEAGSGEYFVR 154
           R+ RD  RV +L+ RL        K     ++   T         +ISG   GSGEYF R
Sbjct: 92  RLNRDTARVKSLITRLDLAINNISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTR 151

Query: 155 IGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSDVCDRL 214
           +G+G P R  YMV+D+GSD+ W+QC PC+ CY Q++P+F+P+ SSS+  +SC +  C+ L
Sbjct: 152 VGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNAL 211

Query: 215 ENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVMIRDVAIGCGHTNQGMFIGAAGLL 274
           E + C    C YEVSYGDGSYT G  A ETLT+G  ++++VA+GCGH+N+G+F+GAAGLL
Sbjct: 212 EVSECRNATCLYEVSYGDGSYTVGDFATETLTIGSTLVQNVAVGCGHSNEGLFVGAAGLL 271

Query: 275 GLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGALEFGRGALPVGATWISLIRNPRAPS 334
           GLGGG ++   QL      +FSYCLV R + S   ++FG    P  A    L+RN +  +
Sbjct: 272 GLGGGLLALPSQLNTT---SFSYCLVDRDSDSASTVDFGTSLSP-DAVVAPLLRNHQLDT 331

Query: 335 FYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVVMDTGTAVTRFPTAAYVAFRDSFTAQT 394
           FYY+GL GI VGG  + +P+ +F++ E G+ G+++D+GTAVTR  T  Y + RDSF   T
Sbjct: 332 FYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGT 391

Query: 395 SNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPARNFLIPVDGGGTFCLAF 454
            +L +A GV++FDTCY+L+   +V VPTV+F+F  G +L LPA+N++IPVD  GTFCLAF
Sbjct: 392 LDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAF 451

Query: 455 APSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
           AP+ S L+IIGN+QQ+G +++FD AN  +GF  N C
Sbjct: 452 APTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

BLAST of CsGy5G019860 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 388.3 bits (996), Expect = 9.2e-108
Identity = 189/399 (47.37%), Postives = 267/399 (66.92%), Query Frame = 0

Query: 95  RMKRDAIRVATLVRRL---------SHGAPAAVKDSRYKVANFATDVISGMEAGSGEYFV 154
           R++RD+ RVA +V ++         S   P   +D+RY+  +  T V+SG   GSGEYF 
Sbjct: 105 RLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFS 164

Query: 155 RIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSDVCDR 214
           RIGVG+P +  Y+V+D+GSD+ W+QC+PC+ CYQQSDPVF+P  SS++  ++C +  C  
Sbjct: 165 RIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSL 224

Query: 215 LENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQV-MIRDVAIGCGHTNQGMFIGAAG 274
           LE + C + +C Y+VSYGDGS+T G LA +T+T G    I +VA+GCGH N+G+F GAAG
Sbjct: 225 LETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAG 284

Query: 275 LLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGALEFGRGALPVGATWISLIRNPRA 334
           LLGLGGG +S   Q+      +FSYCLV R +G + +L+F    L  G     L+RN + 
Sbjct: 285 LLGLGGGVLSITNQM---KATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKI 344

Query: 335 PSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVVMDTGTAVTRFPTAAYVAFRDSFTA 394
            +FYY+GL+G  VGG +V +P+  F +   G+ GV++D GTAVTR  T AY + RD+F  
Sbjct: 345 DTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLK 404

Query: 395 QTSNLPR-APGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPARNFLIPVDGGGTFC 454
            T NL + +  +S+FDTCYD +   +V+VPTV+F+F+ G  L LPA+N+LIPVD  GTFC
Sbjct: 405 LTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFC 464

Query: 455 LAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
            AFAP+ S LSIIGN+QQ+G +I++D +   +G   N C
Sbjct: 465 FAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of CsGy5G019860 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 384.8 bits (987), Expect = 1.0e-106
Identity = 204/425 (48.00%), Postives = 273/425 (64.24%), Query Frame = 0

Query: 63  SGYKSD-NNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKDS 122
           SG  S+ +++  LNL H D LS        F+ R++RD+ RV + +  L+   P      
Sbjct: 62  SGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKS-IATLAAQIPGRNVTH 121

Query: 123 RYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQS 182
             +   F++ V+SG+  GSGEYF R+GVG+P R  YMV+D+GSDIVW+QC PC RCY QS
Sbjct: 122 APRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQS 181

Query: 183 DPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGR--CRYEVSYGDGSYTKGTLALETLTV 242
           DP+FDP  S ++A + C S  C RL++ GCN  R  C Y+VSYGDGS+T G  + ETLT 
Sbjct: 182 DPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF 241

Query: 243 GQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGS- 302
            +  ++ VA+GCGH N+G+F+GAAGLLGLG G +SF GQ G +    FSYCLV R   S 
Sbjct: 242 RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSK 301

Query: 303 TGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRV-SVPEETFQLTEYGTN 362
             ++ FG  A+   A +  L+ NP+  +FYY+GL GI VGG RV  V    F+L + G  
Sbjct: 302 PSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNG 361

Query: 363 GVVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 422
           GV++D+GT+VTR    AY+A RD+F      L RAP  S+FDTC+DL+    V+VPTV  
Sbjct: 362 GVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVL 421

Query: 423 YFSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 482
           +F  G  ++LPA N+LIPVD  G FC AFA +  GLSIIGNIQQ+G ++ +D A+  VGF
Sbjct: 422 HFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 481

BLAST of CsGy5G019860 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 364.8 bits (935), Expect = 1.1e-100
Identity = 200/426 (46.95%), Postives = 269/426 (63.15%), Query Frame = 0

Query: 71  TFKLNLLHRDKLSHVHGHRRG--FNDRMKRDAIRV--ATLVRRLSHGAPAAVKDSRYKVA 130
           +  ++L H D LS          FN R++RD++RV   T +  +S G  A  +  R    
Sbjct: 60  SLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPR-TAG 119

Query: 131 NFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFD 190
            F+  VISG+  GSGEYF+R+GVG+P  N YMV+D+GSD+VW+QC PC  CY Q+D +FD
Sbjct: 120 GFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFD 179

Query: 191 PADSSSFAGVSCGSDVCDRLENTG-CNAGR---CRYEVSYGDGSYTKGTLALETLTVGQV 250
           P  S +FA V CGS +C RL+++  C   R   C Y+VSYGDGS+T+G  + ETLT    
Sbjct: 180 PKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGA 239

Query: 251 MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSR-GTGST-- 310
            +  V +GCGH N+G+F+GAAGLLGLG G +SF  Q   +  G FSYCLV R  +GS+  
Sbjct: 240 RVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSK 299

Query: 311 --GALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRV-SVPEETFQLTEYGT 370
               + FG  A+P  + +  L+ NP+  +FYY+ L GI VGG RV  V E  F+L   G 
Sbjct: 300 PPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGN 359

Query: 371 NGVVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVS 430
            GV++D+GT+VTR    AYVA RD+F    + L RAP  S+FDTC+DL+G  +V+VPTV 
Sbjct: 360 GGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVV 419

Query: 431 FYFSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVG 483
           F+F  G V +LPA N+LIPV+  G FC AFA +   LSIIGNIQQ+G ++++D     VG
Sbjct: 420 FHFGGGEV-SLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVG 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHE34.7e-18169.11Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LS401.3e-10647.37Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ31.4e-10548.00Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LEW36.3e-6936.24Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Q8S9J61.1e-6840.62Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Match NameE-valueIdentityDescription
XP_004147103.10.0100.00protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KGN51568.1 hypothet... [more]
XP_008445900.10.094.61PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo][more]
KAA0034086.10.091.91protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa][more]
XP_038893071.10.091.91protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida][more]
XP_023547721.11.67e-29083.72protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KPR40.0100.00Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G57906... [more]
A0A1S3BEG70.094.61protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5A7SVY20.091.91Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1KA061.26e-28582.34protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1G1D02.26e-28280.17protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
AT3G20015.13.3e-18269.11Eukaryotic aspartyl protease family protein [more]
AT1G25510.16.4e-10947.73Eukaryotic aspartyl protease family protein [more]
AT3G18490.19.2e-10847.37Eukaryotic aspartyl protease family protein [more]
AT1G01300.11.0e-10648.00Eukaryotic aspartyl protease family protein [more]
AT3G61820.11.1e-10046.95Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 143..305
e-value: 4.9E-50
score: 170.2
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 118..305
e-value: 3.7E-51
score: 175.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 312..482
e-value: 3.2E-45
score: 156.0
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 138..482
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 327..478
e-value: 1.4E-32
score: 112.8
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 67..482
NoneNo IPR availablePANTHERPTHR13683:SF838PROTEIN ASPARTIC PROTEASE IN GUARD CELL 2-LIKEcoord: 67..482
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 143..478
score: 45.030022
IPR033873CND41-likeCDDcd05472cnd41_likecoord: 142..482
e-value: 1.6863E-130
score: 378.152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G019860.2CsGy5G019860.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity