CsGy5G016230 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G016230
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationGy14Chr5: 22549152 .. 22551059 (+)
RNA-Seq ExpressionCsGy5G016230
SyntenyCsGy5G016230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCTATGTTTCTCTCATCTCACTCGCAATCCCAAAAGAAAGAAAAAGAAAAAGATATCAATTTGACAAAAAGATTAAGAAAATGAGAACTTTTCTAGTCCTTCTTCTTCTAGCTTTAGTATTTGTGTTCTCATCTCTTTCAGCTTATTCATTACCATTATCAACACGTGGAAGGTGGATCATTGATTCGCAAACAGGTCGTAGAGTGAAACTTGTGTGTGTGAATTGGCCTTCCCACACACAGAGCATGTTGGCAGAAGGCCTCAACCATAGGCCATTAAAAGACCTAGCAGACGAGGCGATCAAGTTAAGATTTAATTGTGTACGACTCACATATGCAACTCATATGTTCACTCGCTATGCAAATAGGACGATTGAGGAAAACTTTGATCTTCTTGATTTAAAACAAGCAAAAGCTGGATTGGCTCAATATAATCCTTTTGTGTTGAATAAGACTATTGCTGAAGCATATGAAGCTGTTGTTGATGTGTTGGGGGCAAGTGGTTTAATGGTTATCGCTGACAATCACATGAGCCAACCAAGATGGTGTTGCTCTCTCGATGATGGCAATGGCTTCTTCGGAAACAACAATTTTGACCCTCAAGAATGGCTACAAGGGCTTAGCTTGGTTGCTCAACGCTTTCGCAACAAATCAACGGTATGTAATTAATTCACATCACGAGATTTTCTTAGATTCTTAAAACTAAACTCAAACTTTTAAGAACATGTATAGTATTTCATTAATTAACAATTTTTTTAATGGCTGTTTAGGTGGTAGGAATGAGTTTACGAAATGAGATACGAGGCTTTATGGAAAATGCAAATGATTGGAACAAATATATAACTCAAGGGGTAACCACGATTCATAACATAAATTCGGAAGTTTTAGTCATTGTTTCAGGGTTAAATTATGACAACGATCTTCGATGCTTAAAGGAAAAGCCTTTGAATGTTGGCACCTTAGACAATAAGTTGGTTTTCGAGGTACACTTATATTCTTTCAGTGGAGATTCCGAGAGCAAGTTTGTAAAACAACCATTGAACAATATATGTGCAAATATTATGAATGGATTTATAGACCATGCTGGGTTTGTGATGCAAGGACCAAACCCGTTTCCATTATTTGTTAGTGAATATGGATATGATCAAAGAGAAGTTAACGATGCTGAAAACCGATTCATGAGTTGCTTCACAGCCCATCTTGCACAAAGAGATTTGGATTGGGCATTGTGGGCTTGGCAAGGTAGCTATTATTTTAGAGAAGGTCAAGCAGAGCCTGGAGAAAGTTTCGGAGTGCTCGACTCTAATTGGACTCAAATTAAGAACCCTAACTTTGTACAAAAGTTTCAACTATTGCAGACCATGTTGCACGGTAACTATATAAATGATTATTACAAGTACATGCAATGATCTATAGTTATATAGTATCAAAGTAACACTTTGTATAACATCTTTTTATGATATATATGCAGATCCAAATTCTAATGCATCGTTCTCATATGTTATATATCATCCACAAAGTAGCCAATGCATCCAAGTCTCCAATGACAACAAAGAAATTTTCCTCACCAATTGCTCCACCCCAACTCGATGGAGTCATAACAATGATGGCACTCCAATTGAGATGTCAAGCACTGGTTTATACTTGAAGGCCAGTGGGGAAGGCCTTGAGGCATCTCTTTCAACTGATACCTTAAGCCAACAAAGTGTTTGGAGTGCCATTTCAAACTCTAAACTTCATTTGGCCACCTTCACTCAAGGTGGAAAGAGCCTTTGTTTGCAAATGGATAGCTCCAACTCTTCAAAAGTTGTGACCAACTCTTGCATTTGCACCAATGGTGATCCAAATTGCCTCCAAGACACCCGAAGCCAATGGTTTGAACTCGTTGAAACCAACACATTATGA

mRNA sequence

ATGGGCTATGTTTCTCTCATCTCACTCGCAATCCCAAAAGAAAGAAAAAGAAAAAGATATCAATTTGACAAAAAGATTAAGAAAATGAGAACTTTTCTAGTCCTTCTTCTTCTAGCTTTAGTATTTGTGTTCTCATCTCTTTCAGCTTATTCATTACCATTATCAACACGTGGAAGGTGGATCATTGATTCGCAAACAGGTCGTAGAGTGAAACTTGTGTGTGTGAATTGGCCTTCCCACACACAGAGCATGTTGGCAGAAGGCCTCAACCATAGGCCATTAAAAGACCTAGCAGACGAGGCGATCAAGTTAAGATTTAATTGTGTACGACTCACATATGCAACTCATATGTTCACTCGCTATGCAAATAGGACGATTGAGGAAAACTTTGATCTTCTTGATTTAAAACAAGCAAAAGCTGGATTGGCTCAATATAATCCTTTTGTGTTGAATAAGACTATTGCTGAAGCATATGAAGCTGTTGTTGATGTGTTGGGGGCAAGTGGTTTAATGGTTATCGCTGACAATCACATGAGCCAACCAAGATGGTGTTGCTCTCTCGATGATGGCAATGGCTTCTTCGGAAACAACAATTTTGACCCTCAAGAATGGCTACAAGGGCTTAGCTTGGTTGCTCAACGCTTTCGCAACAAATCAACGGTGGTAGGAATGAGTTTACGAAATGAGATACGAGGCTTTATGGAAAATGCAAATGATTGGAACAAATATATAACTCAAGGGGTAACCACGATTCATAACATAAATTCGGAAGTTTTAGTCATTGTTTCAGGGTTAAATTATGACAACGATCTTCGATGCTTAAAGGAAAAGCCTTTGAATGTTGGCACCTTAGACAATAAGTTGGTTTTCGAGGTACACTTATATTCTTTCAGTGGAGATTCCGAGAGCAAGTTTGTAAAACAACCATTGAACAATATATGTGCAAATATTATGAATGGATTTATAGACCATGCTGGGTTTGTGATGCAAGGACCAAACCCGTTTCCATTATTTGTTAGTGAATATGGATATGATCAAAGAGAAGTTAACGATGCTGAAAACCGATTCATGAGTTGCTTCACAGCCCATCTTGCACAAAGAGATTTGGATTGGGCATTGTGGGCTTGGCAAGGTAGCTATTATTTTAGAGAAGGTCAAGCAGAGCCTGGAGAAAGTTTCGGAGTGCTCGACTCTAATTGGACTCAAATTAAGAACCCTAACTTTGTACAAAAGTTTCAACTATTGCAGACCATGTTGCACGATCCAAATTCTAATGCATCGTTCTCATATGTTATATATCATCCACAAAGTAGCCAATGCATCCAAGTCTCCAATGACAACAAAGAAATTTTCCTCACCAATTGCTCCACCCCAACTCGATGGAGTCATAACAATGATGGCACTCCAATTGAGATGTCAAGCACTGGTTTATACTTGAAGGCCAGTGGGGAAGGCCTTGAGGCATCTCTTTCAACTGATACCTTAAGCCAACAAAGTGTTTGGAGTGCCATTTCAAACTCTAAACTTCATTTGGCCACCTTCACTCAAGGTGGAAAGAGCCTTTGTTTGCAAATGGATAGCTCCAACTCTTCAAAAGTTGTGACCAACTCTTGCATTTGCACCAATGGTGATCCAAATTGCCTCCAAGACACCCGAAGCCAATGGTTTGAACTCGTTGAAACCAACACATTATGA

Coding sequence (CDS)

ATGGGCTATGTTTCTCTCATCTCACTCGCAATCCCAAAAGAAAGAAAAAGAAAAAGATATCAATTTGACAAAAAGATTAAGAAAATGAGAACTTTTCTAGTCCTTCTTCTTCTAGCTTTAGTATTTGTGTTCTCATCTCTTTCAGCTTATTCATTACCATTATCAACACGTGGAAGGTGGATCATTGATTCGCAAACAGGTCGTAGAGTGAAACTTGTGTGTGTGAATTGGCCTTCCCACACACAGAGCATGTTGGCAGAAGGCCTCAACCATAGGCCATTAAAAGACCTAGCAGACGAGGCGATCAAGTTAAGATTTAATTGTGTACGACTCACATATGCAACTCATATGTTCACTCGCTATGCAAATAGGACGATTGAGGAAAACTTTGATCTTCTTGATTTAAAACAAGCAAAAGCTGGATTGGCTCAATATAATCCTTTTGTGTTGAATAAGACTATTGCTGAAGCATATGAAGCTGTTGTTGATGTGTTGGGGGCAAGTGGTTTAATGGTTATCGCTGACAATCACATGAGCCAACCAAGATGGTGTTGCTCTCTCGATGATGGCAATGGCTTCTTCGGAAACAACAATTTTGACCCTCAAGAATGGCTACAAGGGCTTAGCTTGGTTGCTCAACGCTTTCGCAACAAATCAACGGTGGTAGGAATGAGTTTACGAAATGAGATACGAGGCTTTATGGAAAATGCAAATGATTGGAACAAATATATAACTCAAGGGGTAACCACGATTCATAACATAAATTCGGAAGTTTTAGTCATTGTTTCAGGGTTAAATTATGACAACGATCTTCGATGCTTAAAGGAAAAGCCTTTGAATGTTGGCACCTTAGACAATAAGTTGGTTTTCGAGGTACACTTATATTCTTTCAGTGGAGATTCCGAGAGCAAGTTTGTAAAACAACCATTGAACAATATATGTGCAAATATTATGAATGGATTTATAGACCATGCTGGGTTTGTGATGCAAGGACCAAACCCGTTTCCATTATTTGTTAGTGAATATGGATATGATCAAAGAGAAGTTAACGATGCTGAAAACCGATTCATGAGTTGCTTCACAGCCCATCTTGCACAAAGAGATTTGGATTGGGCATTGTGGGCTTGGCAAGGTAGCTATTATTTTAGAGAAGGTCAAGCAGAGCCTGGAGAAAGTTTCGGAGTGCTCGACTCTAATTGGACTCAAATTAAGAACCCTAACTTTGTACAAAAGTTTCAACTATTGCAGACCATGTTGCACGATCCAAATTCTAATGCATCGTTCTCATATGTTATATATCATCCACAAAGTAGCCAATGCATCCAAGTCTCCAATGACAACAAAGAAATTTTCCTCACCAATTGCTCCACCCCAACTCGATGGAGTCATAACAATGATGGCACTCCAATTGAGATGTCAAGCACTGGTTTATACTTGAAGGCCAGTGGGGAAGGCCTTGAGGCATCTCTTTCAACTGATACCTTAAGCCAACAAAGTGTTTGGAGTGCCATTTCAAACTCTAAACTTCATTTGGCCACCTTCACTCAAGGTGGAAAGAGCCTTTGTTTGCAAATGGATAGCTCCAACTCTTCAAAAGTTGTGACCAACTCTTGCATTTGCACCAATGGTGATCCAAATTGCCTCCAAGACACCCGAAGCCAATGGTTTGAACTCGTTGAAACCAACACATTATGA

Protein sequence

MGYVSLISLAIPKERKRKRYQFDKKIKKMRTFLVLLLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQVSNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSAISNSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNTL*
Homology
BLAST of CsGy5G016230 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 8.6e-110
Identity = 215/542 (39.67%), Postives = 319/542 (58.86%), Query Frame = 0

Query: 36  LLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRPLK 95
           LL AL+ +  +  ++SLPL TRGRWI+D  TG RVKL CVNW  H +  L EGLN  P+ 
Sbjct: 13  LLTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVA 72

Query: 96  DLADEAIKLRFNCVRLTYATHMFTR--YANRTIEENFDLLDLKQAKAGLAQYNPFVLNKT 155
            +A     L FNCVRLTY+ HM TR  Y N T+ + F  L+L +A +G+   NP +L+  
Sbjct: 73  TVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLG 132

Query: 156 IAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLVAQ 215
              AY  VV  L  +G+MVI DNH+S+P+WCC++DDGNGFFG+  F+P  W++GL L+A 
Sbjct: 133 HVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMAT 192

Query: 216 RFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDNDLRC 275
            F N   VV MSLRNE+RG       W++++  G  T+H  N +VLVI+SGL +D DL  
Sbjct: 193 YFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSF 252

Query: 276 LKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVMQGPN 335
           L   P+ +     K+V+E H YSF     +       N++C N    F  + GFV    N
Sbjct: 253 LPVLPVTL-PFKEKIVYEGHWYSFGVPWRTGLP----NDVCKNETGRFKSNVGFVTSSAN 312

Query: 336 --PFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQ---AE 395
               PLF+SE+G DQR VND +NR+++C  A+LA+ DLDWALW   GSYY+R  +    +
Sbjct: 313 ATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKD 372

Query: 396 PGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPN-SNASFSYVIYHPQSSQCIQVSNDN 455
             E++G  + +W++I+NP+F+ + + +Q  + DP  +   +  +IYHP S  C++ S   
Sbjct: 373 FEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVE-SGIG 432

Query: 456 KEIFLTNC-STPTRWSHN-NDGTPIEMSSTGLYLKASGEGLEASLSTD-TLSQQSVWSAI 515
             + L +C S  +RW+++ +   PI +  +   +   G GL A ++ + +    ++WS +
Sbjct: 433 NTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTV 492

Query: 516 SNSKLHLATFTQG--GKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQ--DTRSQWFELVE 563
           S+++L L T   G  GK   + +D S S  + TN CIC   D +C    +   QWF+++ 
Sbjct: 493 SSAQLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICIT-DSHCYPKLNPEKQWFKVIT 547

BLAST of CsGy5G016230 vs. ExPASy Swiss-Prot
Match: P54583 (Endoglucanase E1 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351607 GN=Acel_0614 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.3e-14
Identity = 92/402 (22.89%), Postives = 166/402 (41.29%), Query Frame = 0

Query: 29  MRTFLVLLLLALVFVFSSL-------SAYSLPLSTRGRWIIDSQTGRRVKLVCVNW-PSH 88
           +R  +V+ +LALV   ++L       +A      T GR I+D+     V++  +NW    
Sbjct: 15  LRVGVVVAVLALVAALANLAVPRPARAAGGGYWHTSGREILDA-NNVPVRIAGINWFGFE 74

Query: 89  TQSMLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKA 148
           T + +  GL  R  + + D+   L +N +RL Y+  +       T+  + +   + Q   
Sbjct: 75  TCNYVVHGLWSRDYRSMLDQIKSLGYNTIRLPYSDDIL---KPGTMPNSINFYQMNQDLQ 134

Query: 149 GLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFD 208
           GL      V++K +A A        G  GL +I D H    R  CS    +  +  ++  
Sbjct: 135 GLTSLQ--VMDKIVAYA--------GQIGLRIILDRH----RPDCS--GQSALWYTSSVS 194

Query: 209 PQEWLQGLSLVAQRFRNKSTVVGMSLRNEIR-----GFMENANDWNKYITQGVTTIHNIN 268
              W+  L  +AQR++   TVVG  L NE       G  + + DW     +    + ++N
Sbjct: 195 EATWISDLQALAQRYKGNPTVVGFDLHNEPHDPACWGCGDPSIDWRLAAERAGNAVLSVN 254

Query: 269 SEVLVIVSGL-NYDND-------LRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVK 328
             +L+ V G+ +Y+ D       L+   + P+ V  + N+LV+  H Y+ S   ++ F  
Sbjct: 255 PNLLIFVEGVQSYNGDSYWWGGNLQGAGQYPV-VLNVPNRLVYSAHDYATSVYPQTWFSD 314

Query: 329 QPLNNICANIMNGFIDHAGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCF------T 388
               N    I N    + G++    N  P+++ E+G   +   D    ++         T
Sbjct: 315 PTFPNNMPGIWN---KNWGYLF-NQNIAPVWLGEFGTTLQSTTD--QTWLKTLVQYLRPT 374

Query: 389 AHLAQRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQI 404
           A        W  W+W           + G++ G+L  +W  +
Sbjct: 375 AQYGADSFQWTFWSW---------NPDSGDTGGILKDDWQTV 380

BLAST of CsGy5G016230 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-14
Identity = 97/407 (23.83%), Postives = 164/407 (40.29%), Query Frame = 0

Query: 36  LLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVN-WPSHTQSMLAEGLNHRPL 95
           L LA     ++  A+S  ++   R I+D  +G+ V+L  VN +   T + +  GL  R  
Sbjct: 10  LALATALALAAGPAFSYSIN-NSRQIVD-DSGKVVQLKGVNVFGFETGNHVMHGLWARNW 69

Query: 96  KDLADEAIKLRFNCVRLTYATHMF---TRYANRTIEENFDLLDLKQAKAGLAQYNPFVLN 155
           KD+  +   L FN VRL +        T  A+     N DL  L   +         +L+
Sbjct: 70  KDMIVQMQGLGFNAVRLPFCPATLRSDTMPASIDYSRNADLQGLTSLQ---------ILD 129

Query: 156 KTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLV 215
           K IAE          A G+ V+ D+H      C  + +    +   ++   +WL  L  V
Sbjct: 130 KVIAE--------FNARGMYVLLDHHTPD---CAGISE---LWYTGSYTEAQWLADLRFV 189

Query: 216 AQRFRNKSTVVGMSLRNEIRGFM-----ENANDWNKYITQGVTTIHNINSEVLVIVSGLN 275
           A R++N   V+G+ L+NE  G         A DWNK   +G   +  +  + L+ V G+ 
Sbjct: 190 ANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGIT 249

Query: 276 ------------YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLN--N 335
                       +  +L+ L   PLN+    N+L+   H+Y         FV+   N  N
Sbjct: 250 DNPVCSTNGGIFWGGNLQPLACTPLNIPA--NRLLLAPHVY-----GPDVFVQSYFNDSN 309

Query: 336 ICANIMNGFIDHAGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWA 395
              N+   +  H G   Q      L + E+G    E +  +  +      +L  + ++  
Sbjct: 310 FPNNMPAIWERHFG---QFAGTHALLLGEFGGKYGEGDARDKTWQDALVKYLRSKGINQG 368

Query: 396 L-WAWQGSYYFREGQAEPGESFGVLDSNWTQIKNPNFVQKFQLLQTM 419
             W+W             G++ G+L  +WT ++      K  LL+T+
Sbjct: 370 FYWSW---------NPNSGDTGGILRDDWTSVRQ----DKMTLLRTL 368

BLAST of CsGy5G016230 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 5.7e-13
Identity = 85/411 (20.68%), Postives = 170/411 (41.36%), Query Frame = 0

Query: 24  KKIKKMRTFLVLLLLALVFV---FSSLSAYSLPLS----TRGRWIIDSQTGRRVKLVCVN 83
           KK    +TF V+  L + F    ++ +SA +  +     T+G  I+D ++G+      +N
Sbjct: 2   KKKGLKKTFFVIASLVMGFTLYGYTPVSADAASVKGYYHTQGNKIVD-ESGKEAAFNGLN 61

Query: 84  W-PSHTQSMLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDL 143
           W    T +    GL  R + D+ D+  K  +N +RL Y+  +F   +        D +D 
Sbjct: 62  WFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYSNQLFDSSSRP------DSIDY 121

Query: 144 KQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNG--- 203
            +        NP ++     +  + +++  G  G+ +I D H            G+G   
Sbjct: 122 HK--------NPDLVGLNPIQIMDKLIEKAGQRGIQIILDRHR----------PGSGGQS 181

Query: 204 -FFGNNNFDPQEWLQGLSLVAQRFRNKSTVVGMSLRNEIRGFME----NAN-DWNKYITQ 263
             +  + +    W+    ++A R++N  TV+G  L NE  G       NA+ DW     +
Sbjct: 182 ELWYTSQYPESRWISDWKMLADRYKNNPTVIGADLHNEPHGQASWGTGNASTDWRLAAQR 241

Query: 264 GVTTIHNINSEVLVIVSGLNYD-----------NDLRCLKEKPLNVGTLDNKLVFEVHLY 323
               I ++N   L++V G++++            +L  +   P+ V  + N++V+  H Y
Sbjct: 242 AGNAILSVNPNWLILVEGVDHNVQGNNSQYWWGGNLTGVANYPV-VLDVPNRVVYSPHDY 301

Query: 324 SFSGDSESKFVKQPLNNICANIMNGFIDHAGFVMQGPNPFPLFVSEYGYDQREVNDAENR 383
              G S   +   P     +N+   +    G++ +  N  P+ V E+G    +++  E +
Sbjct: 302 G-PGVSSQPWFNDPA--FPSNLPAIWDQTWGYISK-QNIAPVLVGEFGGRNVDLSCPEGK 361

Query: 384 FMSCFTAHLAQRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQIKNP 407
           + +    ++   +L +  W+              G++ G+L  +WT    P
Sbjct: 362 WQNALVHYIGANNLYFTYWSL---------NPNSGDTGGLLLDDWTTWNRP 373

BLAST of CsGy5G016230 vs. NCBI nr
Match: KAA0042711.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1038 bits (2683), Expect = 0.0
Identity = 509/536 (94.96%), Postives = 520/536 (97.01%), Query Frame = 0

Query: 29  MRTFLVLLLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEG 88
           MRTFL +LL+ALVFVFS LS  SLPLSTRGRWIIDSQTGRRVKLVC+NWPSHTQSML EG
Sbjct: 1   MRTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEG 60

Query: 89  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 148
           LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF
Sbjct: 61  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 120

Query: 149 VLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 208
           VLNKTIAEAYEAVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGNNNFDPQEWLQGL
Sbjct: 121 VLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 180

Query: 209 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 268
           SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD
Sbjct: 181 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 240

Query: 269 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFV 328
           NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLN+ICANIMNGFIDHAGFV
Sbjct: 241 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFV 300

Query: 329 MQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQAE 388
           MQG NPFPLFVSE+GYDQREVNDAENRFMSCFTAHLA RDLDWALWAWQGSYYFREGQAE
Sbjct: 301 MQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAE 360

Query: 389 PGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQVSNDNK 448
           PGESFGVLDSNWTQ+KNPNF +KFQLLQTML DPNSN SFSYVIYHPQSSQCIQVSNDNK
Sbjct: 361 PGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNK 420

Query: 449 EIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSAISNSK 508
           EIFLT+CSTPTRWSH ND TPI+MSSTGL LKASGEGLEASLSTD LSQQSVWSAISNSK
Sbjct: 421 EIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSK 480

Query: 509 LHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNTL 564
           LHLAT TQGGKSLCLQ+DSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELV TNTL
Sbjct: 481 LHLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of CsGy5G016230 vs. NCBI nr
Match: KAA0042709.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1034 bits (2674), Expect = 0.0
Identity = 510/539 (94.62%), Postives = 522/539 (96.85%), Query Frame = 0

Query: 27  KKMRTFLVLLLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLA 86
           +KMRTFLV+LLLALVFVFS L A SLPLSTRGRWIIDSQTGRRVKLVC+NWPSHTQSML 
Sbjct: 2   EKMRTFLVILLLALVFVFSPLFANSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLI 61

Query: 87  EGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYN 146
           EGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA YN
Sbjct: 62  EGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYN 121

Query: 147 PFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQ 206
           PFVLNKTIAEAYEAVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGNNNFDPQEWLQ
Sbjct: 122 PFVLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQ 181

Query: 207 GLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLN 266
           GLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLN
Sbjct: 182 GLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLN 241

Query: 267 YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAG 326
           YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLN+ICANIMNGFIDHAG
Sbjct: 242 YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAG 301

Query: 327 FVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQ 386
           FVMQG NPFPLFVSE+GYDQREVNDAENRFMSCFTAHLA RDLDWALWAWQGSYYFREGQ
Sbjct: 302 FVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQ 361

Query: 387 AEPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQVSND 446
           AEPGESFGVLDSNWTQ+KNPNF +KFQLLQTML DPNSNASFSYVIYHPQSSQCIQVSND
Sbjct: 362 AEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQVSND 421

Query: 447 NKEIFLTNCS-TPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSAIS 506
           NKEIFLT+CS TPTRWSH ND TPI+MSSTGL LKASGEGLEASLSTD LSQQSVWSAIS
Sbjct: 422 NKEIFLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAIS 481

Query: 507 NSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNTL 564
           NSKLHLAT TQGGKSLCLQ+DSSN SKVVTNSCICTNGDPNCLQDTRSQWFELV TNTL
Sbjct: 482 NSKLHLATITQGGKSLCLQIDSSNPSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 540

BLAST of CsGy5G016230 vs. NCBI nr
Match: KAA0042713.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1033 bits (2670), Expect = 0.0
Identity = 506/536 (94.40%), Postives = 517/536 (96.46%), Query Frame = 0

Query: 29  MRTFLVLLLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEG 88
           MRTFL +LLLALVFVFS LS  SLPLSTRGRWIID QTGRRVKLVC+NWPSHTQSML EG
Sbjct: 1   MRTFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEG 60

Query: 89  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 148
           LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF
Sbjct: 61  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 120

Query: 149 VLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 208
           VLNKTIAEAYEAVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGNNNFDPQEWLQGL
Sbjct: 121 VLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 180

Query: 209 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 268
           SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD
Sbjct: 181 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 240

Query: 269 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFV 328
           NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFV
Sbjct: 241 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFV 300

Query: 329 MQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQAE 388
           MQG NPFPLFVSE+GYDQREVNDAENRFMSCFTAHLA RDLDWALWAWQGSYYFREGQAE
Sbjct: 301 MQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAE 360

Query: 389 PGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQVSNDNK 448
           PGESFGVLDSNWTQ+KNPNF +KFQLLQTML DPNSNASFSYVIYHPQ+SQCIQVSNDNK
Sbjct: 361 PGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNK 420

Query: 449 EIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSAISNSK 508
           EIFLT+CSTPTRWSH ND TPI+MSSTGL LKASGEGLEASLSTD L QQSVWSAISNSK
Sbjct: 421 EIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSK 480

Query: 509 LHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNTL 564
           LHLAT  QGG SLCLQ+DSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELV TNTL
Sbjct: 481 LHLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

BLAST of CsGy5G016230 vs. NCBI nr
Match: KAE8648504.1 (hypothetical protein Csa_008732 [Cucumis sativus])

HSP 1 Score: 989 bits (2556), Expect = 0.0
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 84  MLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 143
           MLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA
Sbjct: 1   MLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 60

Query: 144 QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQE 203
           QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQE 120

Query: 204 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 263
           WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS
Sbjct: 121 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 180

Query: 264 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID 323
           GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID
Sbjct: 181 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID 240

Query: 324 HAGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFR 383
           HAGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFR
Sbjct: 241 HAGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFR 300

Query: 384 EGQAEPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQV 443
           EGQAEPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQV
Sbjct: 301 EGQAEPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQV 360

Query: 444 SNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSA 503
           SNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSA
Sbjct: 361 SNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSA 420

Query: 504 ISNSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNT 563
           ISNSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNT
Sbjct: 421 ISNSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNT 480

BLAST of CsGy5G016230 vs. NCBI nr
Match: KAE8650477.1 (hypothetical protein Csa_010217 [Cucumis sativus])

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 470/481 (97.71%), Postives = 475/481 (98.75%), Query Frame = 0

Query: 84  MLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 143
           MLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHM TRYANRTIEENFDLLDLKQAKAGLA
Sbjct: 1   MLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMLTRYANRTIEENFDLLDLKQAKAGLA 60

Query: 144 QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQE 203
           QYNPFVLNKT+AEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQE
Sbjct: 61  QYNPFVLNKTVAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQE 120

Query: 204 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 263
           WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS
Sbjct: 121 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 180

Query: 264 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID 323
           GLNYDNDLRCLKEKPLNV TLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID
Sbjct: 181 GLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID 240

Query: 324 HAGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFR 383
           H GFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHL QRDLDWALWAWQGSYYFR
Sbjct: 241 HVGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLTQRDLDWALWAWQGSYYFR 300

Query: 384 EGQAEPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQV 443
           EGQAEPGESFGVLDSNWTQIKNPNFV+KFQLLQTML DPNSNASFSYVIYHPQSSQCIQV
Sbjct: 301 EGQAEPGESFGVLDSNWTQIKNPNFVRKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQV 360

Query: 444 SNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSA 503
           SNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASG+GLEASLS+DTLSQQSVWSA
Sbjct: 361 SNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGKGLEASLSSDTLSQQSVWSA 420

Query: 504 ISNSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNT 563
           ISNSKLHLATFTQGGKSLCLQ+DSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELV TNT
Sbjct: 421 ISNSKLHLATFTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNT 480

BLAST of CsGy5G016230 vs. ExPASy TrEMBL
Match: A0A5A7TMC9 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002090 PE=3 SV=1)

HSP 1 Score: 1038 bits (2683), Expect = 0.0
Identity = 509/536 (94.96%), Postives = 520/536 (97.01%), Query Frame = 0

Query: 29  MRTFLVLLLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEG 88
           MRTFL +LL+ALVFVFS LS  SLPLSTRGRWIIDSQTGRRVKLVC+NWPSHTQSML EG
Sbjct: 1   MRTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEG 60

Query: 89  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 148
           LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF
Sbjct: 61  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 120

Query: 149 VLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 208
           VLNKTIAEAYEAVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGNNNFDPQEWLQGL
Sbjct: 121 VLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 180

Query: 209 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 268
           SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD
Sbjct: 181 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 240

Query: 269 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFV 328
           NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLN+ICANIMNGFIDHAGFV
Sbjct: 241 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFV 300

Query: 329 MQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQAE 388
           MQG NPFPLFVSE+GYDQREVNDAENRFMSCFTAHLA RDLDWALWAWQGSYYFREGQAE
Sbjct: 301 MQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAE 360

Query: 389 PGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQVSNDNK 448
           PGESFGVLDSNWTQ+KNPNF +KFQLLQTML DPNSN SFSYVIYHPQSSQCIQVSNDNK
Sbjct: 361 PGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNK 420

Query: 449 EIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSAISNSK 508
           EIFLT+CSTPTRWSH ND TPI+MSSTGL LKASGEGLEASLSTD LSQQSVWSAISNSK
Sbjct: 421 EIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSK 480

Query: 509 LHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNTL 564
           LHLAT TQGGKSLCLQ+DSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELV TNTL
Sbjct: 481 LHLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of CsGy5G016230 vs. ExPASy TrEMBL
Match: A0A5A7TMX0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002070 PE=3 SV=1)

HSP 1 Score: 1034 bits (2674), Expect = 0.0
Identity = 510/539 (94.62%), Postives = 522/539 (96.85%), Query Frame = 0

Query: 27  KKMRTFLVLLLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLA 86
           +KMRTFLV+LLLALVFVFS L A SLPLSTRGRWIIDSQTGRRVKLVC+NWPSHTQSML 
Sbjct: 2   EKMRTFLVILLLALVFVFSPLFANSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLI 61

Query: 87  EGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYN 146
           EGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA YN
Sbjct: 62  EGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYN 121

Query: 147 PFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQ 206
           PFVLNKTIAEAYEAVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGNNNFDPQEWLQ
Sbjct: 122 PFVLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQ 181

Query: 207 GLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLN 266
           GLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLN
Sbjct: 182 GLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLN 241

Query: 267 YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAG 326
           YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLN+ICANIMNGFIDHAG
Sbjct: 242 YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAG 301

Query: 327 FVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQ 386
           FVMQG NPFPLFVSE+GYDQREVNDAENRFMSCFTAHLA RDLDWALWAWQGSYYFREGQ
Sbjct: 302 FVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQ 361

Query: 387 AEPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQVSND 446
           AEPGESFGVLDSNWTQ+KNPNF +KFQLLQTML DPNSNASFSYVIYHPQSSQCIQVSND
Sbjct: 362 AEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQVSND 421

Query: 447 NKEIFLTNCS-TPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSAIS 506
           NKEIFLT+CS TPTRWSH ND TPI+MSSTGL LKASGEGLEASLSTD LSQQSVWSAIS
Sbjct: 422 NKEIFLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAIS 481

Query: 507 NSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNTL 564
           NSKLHLAT TQGGKSLCLQ+DSSN SKVVTNSCICTNGDPNCLQDTRSQWFELV TNTL
Sbjct: 482 NSKLHLATITQGGKSLCLQIDSSNPSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 540

BLAST of CsGy5G016230 vs. ExPASy TrEMBL
Match: A0A5A7THG1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002110 PE=3 SV=1)

HSP 1 Score: 1033 bits (2670), Expect = 0.0
Identity = 506/536 (94.40%), Postives = 517/536 (96.46%), Query Frame = 0

Query: 29  MRTFLVLLLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEG 88
           MRTFL +LLLALVFVFS LS  SLPLSTRGRWIID QTGRRVKLVC+NWPSHTQSML EG
Sbjct: 1   MRTFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEG 60

Query: 89  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 148
           LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF
Sbjct: 61  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 120

Query: 149 VLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 208
           VLNKTIAEAYEAVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGNNNFDPQEWLQGL
Sbjct: 121 VLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 180

Query: 209 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 268
           SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD
Sbjct: 181 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 240

Query: 269 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFV 328
           NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFV
Sbjct: 241 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFV 300

Query: 329 MQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQAE 388
           MQG NPFPLFVSE+GYDQREVNDAENRFMSCFTAHLA RDLDWALWAWQGSYYFREGQAE
Sbjct: 301 MQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAE 360

Query: 389 PGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQVSNDNK 448
           PGESFGVLDSNWTQ+KNPNF +KFQLLQTML DPNSNASFSYVIYHPQ+SQCIQVSNDNK
Sbjct: 361 PGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNK 420

Query: 449 EIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSAISNSK 508
           EIFLT+CSTPTRWSH ND TPI+MSSTGL LKASGEGLEASLSTD L QQSVWSAISNSK
Sbjct: 421 EIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSK 480

Query: 509 LHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNTL 564
           LHLAT  QGG SLCLQ+DSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELV TNTL
Sbjct: 481 LHLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

BLAST of CsGy5G016230 vs. ExPASy TrEMBL
Match: A0A0A0K853 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3 SV=1)

HSP 1 Score: 956 bits (2471), Expect = 0.0
Identity = 467/536 (87.13%), Postives = 496/536 (92.54%), Query Frame = 0

Query: 30  RTFLVLLLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGL 89
           RT  V+LLLALV VFSS SAYSLPLST GRWIIDSQ+G+RVKLVCVNWPSHTQSML EGL
Sbjct: 3   RTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGL 62

Query: 90  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 149
           NHRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNPFV
Sbjct: 63  NHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFV 122

Query: 150 LNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 209
           LNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGN  FDPQEWLQGLS
Sbjct: 123 LNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLS 182

Query: 210 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 269
           LVAQRF NKSTVVGMSLRNE+RG MENANDWN Y+TQGVTTIH IN  VLVIVSGLNYDN
Sbjct: 183 LVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDN 242

Query: 270 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVM 329
           DLRCLK+KPLNV TLDNKL FEVHLYSFSGDSESKFV+QPLNNICA IM+ FIDHA FV+
Sbjct: 243 DLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVI 302

Query: 330 QGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQAEP 389
           +GPNPFPLFVSEYGYDQREV+DAENRFMSCFTAHLAQ+DLDWALW WQGSYY+REGQAE 
Sbjct: 303 EGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEL 362

Query: 390 GESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQVSNDNKE 449
            E+FGVLDSNWTQIKNPNFVQKFQLLQTML DP SNASFSYVIYH QS QCI+VSNDNKE
Sbjct: 363 AETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDNKE 422

Query: 450 IFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSAISNSKL 509
           IFLTNCST +RWSH+ND TPI+MSSTGL LKASGEGLEASLSTD + +QS+WSAISNS L
Sbjct: 423 IFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNSNL 482

Query: 510 HLATFTQGGKSLCLQM-DSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNTL 564
           HL T T+ GKSLCLQ+ +SSNSSK+VTNSCICT  DP CLQDT+SQWFELV TNTL
Sbjct: 483 HLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

BLAST of CsGy5G016230 vs. ExPASy TrEMBL
Match: A0A5A7TH83 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002080 PE=3 SV=1)

HSP 1 Score: 944 bits (2440), Expect = 0.0
Identity = 458/481 (95.22%), Postives = 466/481 (96.88%), Query Frame = 0

Query: 84  MLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 143
           ML EGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA
Sbjct: 1   MLIEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 60

Query: 144 QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQE 203
           QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGNNNFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQE 120

Query: 204 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 263
           WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVT IHNINSEVLVIVS
Sbjct: 121 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTAIHNINSEVLVIVS 180

Query: 264 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID 323
           GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLN+ICANIMNGFID
Sbjct: 181 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFID 240

Query: 324 HAGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFR 383
           HAGFVMQG NPFPLFVSEYGYDQREVNDAENRFMSCFTAHL  RDLDWALWAWQGSYYFR
Sbjct: 241 HAGFVMQGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLVLRDLDWALWAWQGSYYFR 300

Query: 384 EGQAEPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQV 443
           EGQAEPGESFGVLDSNWTQ+KNPNF +KFQLLQTML DP SN SFSY+IYHPQS QCIQV
Sbjct: 301 EGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPISNTSFSYIIYHPQSGQCIQV 360

Query: 444 SNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSA 503
           SNDNKEIFLTNCSTPTRWSHNNDGTPI+MSSTGL LKASGEGLE SLSTD LSQQSVWSA
Sbjct: 361 SNDNKEIFLTNCSTPTRWSHNNDGTPIKMSSTGLCLKASGEGLETSLSTDCLSQQSVWSA 420

Query: 504 ISNSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNT 563
           ISNSKLHLATFTQGGKSLCLQ+D S+SSKVVTNSCICTNGDPNCLQDTRSQWFELV TNT
Sbjct: 421 ISNSKLHLATFTQGGKSLCLQIDGSSSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNT 480

BLAST of CsGy5G016230 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 411.0 bits (1055), Expect = 1.6e-114
Identity = 211/515 (40.97%), Postives = 316/515 (61.36%), Query Frame = 0

Query: 51  SLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRPLKDLADEAIKLRFNCVR 110
           S PLST  RWI+D + G RVKLVC NWPSH Q ++AEGL+ +P+  +A + +++ FNCVR
Sbjct: 32  SYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNCVR 91

Query: 111 LTYATHMFTRYA---NRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGA 170
           LT+   + T      N T+ ++F  L L     G    NP +++  + EAY+ VV  LG 
Sbjct: 92  LTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTLGN 151

Query: 171 SGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLVAQRFRNKSTVVGMSLR 230
           + +MVI DNH+++P WCC+ DDGNGFFG+  FDP  W+  L  +A  F   S VVGMSLR
Sbjct: 152 NDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVVGMSLR 211

Query: 231 NEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNK 290
           NE+RG  +N NDW KY+ QG   +H+ N++VLVI+SGL++D DL  ++ +P+ + +   K
Sbjct: 212 NELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKL-SFTGK 271

Query: 291 LVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVMQGPNPFPLFVSEYGYDQR 350
           LVFE+H YSFS D  S     P N+IC  ++N   +  G+++     FPLF+SE+G D+R
Sbjct: 272 LVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGYLLN--QGFPLFLSEFGIDER 331

Query: 351 EVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQIKNPN 410
            VN  +NR+  C T   A+ D+DW+LWA  GSYY R+G+    E +GVLDS+W  ++N +
Sbjct: 332 GVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNSS 391

Query: 411 FVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQVS-NDNKEIFLTNCSTPTRWSHNND 470
           F+QK   LQ+ L  P        +++HP +  CI  S +D K + L  C++   WS+   
Sbjct: 392 FLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDDPKMLTLGPCNSSEPWSYTKK 451

Query: 471 GTPIEMSSTGLYLKASGEGLEASLS-TDTLSQQSVWSAISNSKLHLATFTQGGKSLCLQM 530
              + +    L L+++G     +++ T   +  S W  IS S++HLA+ T    SLCL +
Sbjct: 452 A--LRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLDV 511

Query: 531 DSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVE 561
           D++N+  VV N+C C + D +C  +  SQWF++++
Sbjct: 512 DTANN--VVANACKCLSKDKSC--EPMSQWFKIIK 534

BLAST of CsGy5G016230 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 386.3 bits (991), Expect = 4.1e-107
Identity = 219/542 (40.41%), Postives = 314/542 (57.93%), Query Frame = 0

Query: 29  MRTFLVLLLLALVFVFSSLSAYSLPLSTRGRWII-DSQTGRRVKLVCVNWPSHTQSMLAE 88
           M  F  + +  L +V   ++ ++ P ST  RWI+ D   GRRVKL CVNWPSH ++ +AE
Sbjct: 1   MEKFFFISVFLLPYV---ITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAE 60

Query: 89  GLNHRPLKDLADEAIKLRFNCVRLTYATHMFTR---YANRTIEENFDLLDLKQAKAGLAQ 148
           GL+ +PL  +A++ + + FNCVRLT+  ++ T     A  T+ ++     L +A +G   
Sbjct: 61  GLSKQPLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQT 120

Query: 149 YNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEW 208
           +NP +L+  + +A++ VV  L    +MVI DNH+SQP WCCS +DGNGFFG+ + +PQ W
Sbjct: 121 HNPTILDLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVW 180

Query: 209 LQGLSLVAQRFRN-KSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 268
           ++GL  +A  F N  S VVGMSLRNE+RG  +N  DW KY+ +G   +H++N  VLVIVS
Sbjct: 181 IKGLKKMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVS 240

Query: 269 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID 328
           GLNY  DL  L+E+P  V +   K+VFE+H Y F    E       LN IC       + 
Sbjct: 241 GLNYATDLSFLRERPFEV-SFRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKMMK 300

Query: 329 HAGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFR 388
            +GF+++     PLFVSE+G DQR  N  +N+F+SCF A  A RDLDW+LW   GSYY R
Sbjct: 301 MSGFLLE--KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIR 360

Query: 389 EGQAEPGESFGVLDSNWTQIKNPNFVQKFQLLQT-----MLHDPNSNASFSYVIYHPQSS 448
           E      ES+GVLD NW+ I+N   +Q    +QT     M   P        +++HP + 
Sbjct: 361 EKSIGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK------IMFHPSTG 420

Query: 449 QCIQVSNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLST-DTLSQ 508
            CI V     ++ L +C+    W  ++           L LKA  +G    L    + S 
Sbjct: 421 LCI-VRKSLFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESY 480

Query: 509 QSVWSAISNSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFE 560
            S W   S+SK+ L++ T+ G S+CL +D+ N++ +VTNSC C  G+ +C  D RSQWF+
Sbjct: 481 CSKWKLFSDSKMQLSSITKNGFSVCLDVDTENNN-IVTNSCKCLRGNSSC--DPRSQWFK 522

BLAST of CsGy5G016230 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 379.8 bits (974), Expect = 3.8e-105
Identity = 202/517 (39.07%), Postives = 307/517 (59.38%), Query Frame = 0

Query: 53  PLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRPLKDLADEAIKLRFNCVRLT 112
           PLST  RWIID + G+RVKL CVNWPSH Q ++AEGL+ + + DLA + + + FNCVR T
Sbjct: 4   PLSTNSRWIID-EKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 113 YATHMFTRYA---NRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASG 172
           +   + T      N T+ ++F  L L    +G    NP +++  + EAY+ VV  LG + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 173 LMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLVAQRFRNKSTVVGMSLRNE 232
           +MVI DNH+++P WCC  +DGNGFFG+  FDP  W+ GL+ +A  F+  + VVGMSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 233 IRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLV 292
           +RG  +N +DW KY+ QG   +H  N  VLVI+SGL+YD DL  ++ + +N+ T   KLV
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKLV 243

Query: 293 FEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVMQGPNPFPLFVSEYGYDQREV 352
           FE+H YSF+ ++ +   K P N  C  I+    +  GF ++    FP+F+SE+G D R  
Sbjct: 244 FELHRYSFT-NTNTWSSKNP-NEACGEILKSIENGGGFNLR---DFPVFLSEFGIDLRGK 303

Query: 353 NDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQIKNPNFV 412
           N  +NR++ C     A+ D+DW++W  QGSYY REG     E +G+LDS+W ++++ +F+
Sbjct: 304 NVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFL 363

Query: 413 QKFQLLQTMLHDPNSNASFSYVIYHPQSSQC-IQVSNDNKEIFLTNCSTPTRWSHNNDGT 472
           Q+  L+ + L  P S +    +++HP +  C +Q   D  ++ L  C+    WS+    T
Sbjct: 364 QRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQNT 423

Query: 473 PIEMSSTGLYLKASGEGLEASLSTDTLSQQ--SVWSAISNSKLHLATFTQGGKSLCLQMD 532
            + +    L L+++G      LS  + S    S W  IS S + LA       SLCL +D
Sbjct: 424 -LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAA-KSTNNSLCLDVD 483

Query: 533 SSNSSKVVTNSCICTNG-DPNCLQDTRSQWFELVETN 563
            +N+  ++ ++C C  G D +C  D  SQWF++V+ +
Sbjct: 484 ETNN--LMASNCKCVKGEDSSC--DPISQWFKIVKVS 507

BLAST of CsGy5G016230 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 3.1e-99
Identity = 199/541 (36.78%), Postives = 308/541 (56.93%), Query Frame = 0

Query: 35  LLLLALVFVFSSLSAYSL----PLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLN 94
           + L   +F+F SL + +L    PL T+ RWI++++ G RVKL C NWPSH + ++AEGL+
Sbjct: 5   IALTVFLFLFLSLISLTLATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVAEGLS 64

Query: 95  HRPLKDLADEAIKLRFNCVRLTYATHMF---TRYANRTIEENFDLLDLKQAKAGLAQYNP 154
            +P+  ++ +   + FNCVRLT+   +    T   N T++++F+   L     G+  +NP
Sbjct: 65  SQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNP 124

Query: 155 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQG 214
           +++N  +   ++AVV  LG   +MVI DNH + P WCCS DD + FFG+  F+P  W+ G
Sbjct: 125 YIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLG 184

Query: 215 LSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNY 274
           L  +A  F N   VVGMSLRNE+RG+   + DW KY+ +G   +H  N  VLVI+SGLN+
Sbjct: 185 LKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNF 244

Query: 275 DNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGF 334
           D DL  LK++P+N+ +   KLV E+H YSF+ D   ++    +N+ C+ + +      GF
Sbjct: 245 DADLSFLKDRPVNL-SFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERRTGGF 304

Query: 335 VMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQA 394
           V+     FPLF+SE+G DQR  +   NR+M+C  A  A++DLDWA+WA  G YYFREG+ 
Sbjct: 305 VLD--QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKR 364

Query: 395 EPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQVSN-- 454
              E++G+LD+NW  + N  ++++  ++Q     P    +    I+HP +  C+   +  
Sbjct: 365 GVVEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHC 424

Query: 455 DNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSV----- 514
              E+ L  C+    WS+++ G           +     G ++ L  +T   +SV     
Sbjct: 425 HESELTLGPCTKDEPWSYSHGG-----------ILEIRRGHKSCLEGETAVGKSVKLGRI 484

Query: 515 ---WSAISNSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFE 559
                 IS +K+HL+  T  G  +CL +DS N+  VV NSC C  GD  C  +  SQWF+
Sbjct: 485 CTKIEQISATKMHLSFNTSDGSLVCLDVDSDNN--VVANSCNCLTGDTTC--EPASQWFK 525

BLAST of CsGy5G016230 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 304.7 bits (779), Expect = 1.6e-82
Identity = 189/536 (35.26%), Postives = 290/536 (54.10%), Query Frame = 0

Query: 30  RTFLVLLLLALVFVFSSLSAYSLPLSTRGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGL 89
           R+F   L    +   S L+  S PLST+ RWI+D + G+RVKL CVNWP+H Q  +AEGL
Sbjct: 3   RSFYFCLFFLFISSTSKLTT-SYPLSTKSRWIVD-EKGQRVKLACVNWPAHLQPTVAEGL 62

Query: 90  NHRPLKDLADEAIKLRFNCVRLTYATHMFTR---YANRTIEENFDLLDLKQAKAGLAQYN 149
           + +PL  ++ + + + FNCVRLT+   + T        T++++F+ L L +   G+  +N
Sbjct: 63  SKQPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHN 122

Query: 150 PFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQEWLQ 209
           P +L+  +  A++ VV  LG +G+MVI DNH++ P WCC  +D + FFG  +FDP  W +
Sbjct: 123 PKLLHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAK 182

Query: 210 GLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLN 269
           GL  +A  FRN + V+GMSLRNE RG  +  + W +++ QG   +H  N ++LVI+SG++
Sbjct: 183 GLRKMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGID 242

Query: 270 YDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAG 329
           +D +L  L+++ +NV   D KLVFE+H YSFS D    + K   N+ C  I+     + G
Sbjct: 243 FDTNLSFLRDRSVNVSFTD-KLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHNGG 302

Query: 330 FVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFREGQ 389
           F++     FPL +SE+G DQR  + + NR+M+C  A  A+ DLDWA+WA  G YY R G 
Sbjct: 303 FLL--GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTG- 362

Query: 390 AEPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCI--QVS 449
             PG                               PN N     +++HP +  C+    S
Sbjct: 363 --PGLR-----------------------------PNKN-----LLFHPSTGLCVTNNPS 422

Query: 450 NDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASG-EGLEASLSTDTLSQQSVWSA 509
           ++   + L  C     W+ N     + ++   + ++A    G +  L   T  + S    
Sbjct: 423 DNIPTLRLGPCPKSDPWTFNPSEGILWINK--MCVEAPNVVGQKVKLGVGT--KCSKLGQ 482

Query: 510 ISNSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELV 560
           IS +K+HL+  T  G  LCL +D  ++S VV N C     D +C  D  SQWF+++
Sbjct: 483 ISATKMHLSFKTSNGLLLCLDVDERDNS-VVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA08.6e-11039.67Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P545832.3e-1422.89Endoglucanase E1 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351... [more]
P194873.0e-1423.83Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
P235485.7e-1320.68Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
KAA0042711.10.094.96major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
KAA0042709.10.094.62major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
KAA0042713.10.094.40major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
KAE8648504.10.0100.00hypothetical protein Csa_008732 [Cucumis sativus][more]
KAE8650477.10.097.71hypothetical protein Csa_010217 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A5A7TMC90.094.96Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7TMX00.094.62Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7THG10.094.40Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A0A0K8530.087.13Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3... [more]
A0A5A7TH830.095.22Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
AT1G13130.11.6e-11440.97Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.14.1e-10740.41Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.13.8e-10539.07Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.13.1e-9936.78Glycosyl hydrolase superfamily protein [more]
AT5G16700.11.6e-8235.26Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 90..377
e-value: 2.0E-24
score: 86.5
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 52..423
e-value: 3.2E-67
score: 229.2
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 35..562
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 53..412
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 426..539

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G016230.2CsGy5G016230.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds