CsGy5G009647 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy5G009647
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Cystatin domain-containing protein
Location	Gy14Chr5: 8360378 .. 8360898 (-)
RNA-Seq Expression	CsGy5G009647
Synteny	CsGy5G009647

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTCTAATTAACCATATCAAACGATCCAAATATCATGAGTTGTGTACAAGTACTTCCAAATACTCATGACCTGATTAAGGATGTCCATAGCCCAAACGTGATAGAAGCTGGAAAGATAGCAGTGGATGAACATAACAAGAAAGAAAATGACAATTTAGTTTTCACTCGTGTAGTGAGTGGTTTAGCACCCCGTGCTATTATTGGAACTATATTATGGTCATATATTCTCGTGATAGAAGCTAAAAACTGCGACGGATATCCTGTGGCATATGTGGCTAAAATAGTTAAGTATCTTACAATTCCAGAAAATAGCTATGAACTTGTCAGCTTTCAAGCTATACTCGGGTATAAGTAGTATTAGATTGCTCTCCCTGTCTTCAGAATAATATGTCCTTTATTTATATATATATATGAAAAGTAAGGGGCATTATTGTGTTTAATTACCTATATGAAAATGGTATAGCCATATATACTGAAATAATCAATGGTACTTACCTAATAATGGATTACATGTCTCCT

mRNA sequence

CTTCTCTAATTAACCATATCAAACGATCCAAATATCATGAGTTGTGTACAAGTACTTCCAAATACTCATGACCTGATTAAGGATGTCCATAGCCCAAACGTGATAGAAGCTGGAAAGATAGCAGTGGATGAACATAACAAGAAAGAAAATGACAATTTAGTTTTCACTCGTGTAGTGAGTGGTTTAGCACCCCGTGCTATTATTGGAACTATATTATGGTCATATATTCTCGTGATAGAAGCTAAAAACTGCGACGGATATCCTGTGGCATATGTGGCTAAAATAGTTAAGTATCTTACAATTCCAGAAAATAGCTATGAACTTGTCAGCTTTCAAGCTATACTCGGGTATAAGTAGTATTAGATTGCTCTCCCTGTCTTCAGAATAATATGTCCTTTATTTATATATATATATGAAAAGTAAGGGGCATTATTGTGTTTAATTACCTATATGAAAATGGTATAGCCATATATACTGAAATAATCAATGGTACTTACCTAATAATGGATTACATGTCTCCT

Coding sequence (CDS)

ATGAGTTGTGTACAAGTACTTCCAAATACTCATGACCTGATTAAGGATGTCCATAGCCCAAACGTGATAGAAGCTGGAAAGATAGCAGTGGATGAACATAACAAGAAAGAAAATGACAATTTAGTTTTCACTCGTGTAGTGAGTGGTTTAGCACCCCGTGCTATTATTGGAACTATATTATGGTCATATATTCTCGTGATAGAAGCTAAAAACTGCGACGGATATCCTGTGGCATATGTGGCTAAAATAGTTAAGTATCTTACAATTCCAGAAAATAGCTATGAACTTGTCAGCTTTCAAGCTATACTCGGGTATAAGTAG

Protein sequence

MSCVQVLPNTHDLIKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNCDGYPVAYVAKIVKYLTIPENSYELVSFQAILGYK*

Homology

[Close]

BLAST of CsGy5G009647 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 3.7e-06
Identity = 34/89 (38.20%), Postives = 50/89 (56.18%), Query Frame = 0


Query: 14  IKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNCD 73
           IK+V  P+V+   K A++EHNK+  + LVF +VV G   + + GT    Y L I AK+  
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEG-TTQVVSGT---KYDLKIAAKDGG 94

Query: 74  GYPVAYVAKIVKYLTIPENSYELVSFQAI 103
           G    Y A +V+ L +   S E  SF+A+
Sbjct: 95  GKIKNYEAVVVEKLWLHSKSLE--SFKAL 117

[Close]

BLAST of CsGy5G009647 vs. NCBI nr
Match: KAE8648095.1 (hypothetical protein Csa_004720 [Cucumis sativus])

HSP 1 Score: 216 bits (550), Expect = 9.08e-71
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 0


Query: 1   MSCVQVLPNTHDLIKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTIL 60
           MSCVQVLPNTHDLIKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTIL
Sbjct: 1   MSCVQVLPNTHDLIKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTIL 60

Query: 61  WSYILVIEAKNCDGYPVAYVAKIVKYLTIPENSYELVSFQAILGYK 106
           WSYILVIEAKNCDGYPVAYVAKIVKYLTIPENSYELVSFQAILGYK
Sbjct: 61  WSYILVIEAKNCDGYPVAYVAKIVKYLTIPENSYELVSFQAILGYK 106

[Close]

BLAST of CsGy5G009647 vs. NCBI nr
Match: KAE8648094.1 (hypothetical protein Csa_004738 [Cucumis sativus])

HSP 1 Score: 169 bits (428), Expect = 3.69e-52
Identity = 79/106 (74.53%), Postives = 93/106 (87.74%), Query Frame = 0


Query: 1   MSCVQVLPNTHDLIKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTIL 60
           MSCVQV PNTHDLIKDVHSP+V+EAGK+AV+EHNKKENDNLVF RVVS +APRA+I   L
Sbjct: 1   MSCVQVPPNTHDLIKDVHSPDVVEAGKVAVEEHNKKENDNLVFIRVVSAIAPRALIFIKL 60

Query: 61  WSYILVIEAKNCDGYPVAYVAKIVKYLTIPENSYELVSFQAILGYK 106
           WS+ILVIEAK C+G+PV YV +I KYLTIPE SY+L+ F+A+L YK
Sbjct: 61  WSFILVIEAKTCNGFPVTYVVEIDKYLTIPEESYKLIRFEAVLEYK 106

[Close]

BLAST of CsGy5G009647 vs. NCBI nr
Match: XP_010434812.1 (PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa])

HSP 1 Score: 59.7 bits (143), Expect = 8.48e-09
Identity = 36/89 (40.45%), Postives = 51/89 (57.30%), Query Frame = 0


Query: 14  IKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNCD 73
           IKD+  PNV+   K A++EHNK+  +NLVF +VV G   + + GT    Y + I AKN  
Sbjct: 32  IKDLSDPNVVAVAKYAIEEHNKQSKENLVFVKVVEG-TTQVVSGT---KYDMKIAAKNGG 91

Query: 74  GYPVAYVAKIVKYLTIPENSYELVSFQAI 102
           G    Y A +V+ L +   S E  SF+A+
Sbjct: 92  GQIKNYEAVVVEKLWLRSKSLE--SFKAV 114

[Close]

BLAST of CsGy5G009647 vs. NCBI nr
Match: XP_010449778.1 (PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa])

HSP 1 Score: 59.3 bits (142), Expect = 1.20e-08
Identity = 37/89 (41.57%), Postives = 50/89 (56.18%), Query Frame = 0


Query: 14  IKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNCD 73
           IKDV  PNV+     A++EHNK+  +NLVF +VV G   + + GT    Y L I AKN  
Sbjct: 32  IKDVSDPNVVAIANYAIEEHNKQSKENLVFVKVVEG-TTQVVSGT---KYDLKIAAKNGG 91

Query: 74  GYPVAYVAKIVKYLTIPENSYELVSFQAI 102
           G    Y A +V+ L +   S E  SF+A+
Sbjct: 92  GQIKNYEAVVVEKLWLRSKSLE--SFKAV 114

[Close]

BLAST of CsGy5G009647 vs. NCBI nr
Match: KAF8093897.1 (hypothetical protein N665_0375s0014 [Sinapis alba])

HSP 1 Score: 58.5 bits (140), Expect = 2.23e-08
Identity = 29/62 (46.77%), Postives = 40/62 (64.52%), Query Frame = 0


Query: 13 LIKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNC 72
          LIKDV +PNV E  K A+DEHN + N+NLVF  ++ G   R ++  I++   L+I AKN 
Sbjct: 30 LIKDVSAPNVKEVAKYAIDEHNSESNENLVFVEIIEG--KRQLLSAIIYD--LIIAAKNN 87

Query: 73 DG 74
           G
Sbjct: 90 GG 87

[Close]

BLAST of CsGy5G009647 vs. ExPASy TrEMBL
Match: A0A6J0MCI3 (cysteine proteinase inhibitor 4-like OS=Raphanus sativus OX=3726 GN=LOC108840944 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.08e-08
Identity = 29/61 (47.54%), Postives = 39/61 (63.93%), Query Frame = 0


Query: 14 IKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNCD 73
          IKDV +PNVIE  K A+DEHN + NDNL+F  +V G   R ++  I++   L+I  KN  
Sbjct: 31 IKDVSAPNVIEVAKYAIDEHNSETNDNLMFVEIVEG--KREVLSAIIYD--LIIAVKNEG 87

[Close]

BLAST of CsGy5G009647 vs. ExPASy TrEMBL
Match: A0A068VPR8 (Cystatin domain-containing protein OS=Coffea canephora OX=49390 GN=GSCOC_T00008667001 PE=4 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 1.12e-07
Identity = 27/55 (49.09%), Postives = 36/55 (65.45%), Query Frame = 0


Query: 20  PNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNCDG 74
           P++ E G+ AVDEHNK+  D LVF  VV+GL     + T+ W   L+IEAK+ DG
Sbjct: 60  PHIQELGRFAVDEHNKQTGDKLVFVAVVAGLKKPVELATLYW---LIIEAKDSDG 111

[Close]

BLAST of CsGy5G009647 vs. ExPASy TrEMBL
Match: A0A6P6XFN5 (cysteine proteinase inhibitor-like OS=Coffea arabica OX=13443 GN=LOC113742174 PE=4 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 1.12e-07
Identity = 27/55 (49.09%), Postives = 36/55 (65.45%), Query Frame = 0


Query: 20  PNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNCDG 74
           P++ E G+ AVDEHNK+  D LVF  VV+GL     + T+ W   L+IEAK+ DG
Sbjct: 60  PHIQELGRFAVDEHNKQTGDKLVFVAVVAGLKKPVELATLYW---LIIEAKDSDG 111

[Close]

BLAST of CsGy5G009647 vs. ExPASy TrEMBL
Match: A0A6J1EK72 (uncharacterized protein LOC111433339 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433339 PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.12e-07
Identity = 32/83 (38.55%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 1  MSCVQVLPNTHDLIKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTIL 60
          MSC  V P     I+ V+   V EA + A+   N+KE   L++ RV++GL+         
Sbjct: 1  MSCEPVFPPVPYAIRGVNHKKVQEAAEAAIKWKNEKERRCLIYKRVINGLSDSKCNHEDS 60

Query: 61 WSYILVIEAKNCDGYPVAYVAKI 83
            Y+LVIEA N DG P +Y+AK+
Sbjct: 61 QHYVLVIEAINDDGIPWSYIAKV 83

[Close]

BLAST of CsGy5G009647 vs. ExPASy TrEMBL
Match: A0A484MG82 (Cystatin domain-containing protein OS=Cuscuta campestris OX=132261 GN=CCAM_LOCUS29596 PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.46e-07
Identity = 35/87 (40.23%), Postives = 48/87 (55.17%), Query Frame = 0


Query: 16  DVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNCDGY 75
           D  SP V+ AG  AVD HNK ENDNL F  V SG   + + G   + Y LVI+A++  G 
Sbjct: 37  DPESPEVVAAGNFAVDAHNKAENDNLGFEDVASG-EEQVVAG---FQYKLVIDARSGHG- 96

Query: 76  PVAYVAKIVKYLTIPENSYELVSFQAI 102
              Y  + V +     +SY L+SF+ +
Sbjct: 97  --VYQYEAVVWAKPSPDSYSLISFKQL 116

[Close]

BLAST of CsGy5G009647 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 2.7e-07
Identity = 34/89 (38.20%), Postives = 50/89 (56.18%), Query Frame = 0


Query: 14  IKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNCD 73
           IK+V  P+V+   K A++EHNK+  + LVF +VV G   + + GT    Y L I AK+  
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEG-TTQVVSGT---KYDLKIAAKDGG 94

Query: 74  GYPVAYVAKIVKYLTIPENSYELVSFQAI 103
           G    Y A +V+ L +   S E  SF+A+
Sbjct: 95  GKIKNYEAVVVEKLWLHSKSLE--SFKAL 117

[Close]

BLAST of CsGy5G009647 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 2.7e-04
Identity = 26/68 (38.24%), Postives = 37/68 (54.41%), Query Frame = 0


Query: 14 IKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTILWSYILVIEAKNCD 73
          I +V  P V+E G+ AV E+NK+    L F  VVSG   + + GT   +Y L + A + D
Sbjct: 34 ISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSG-ETQVVSGT---NYRLKVAANDGD 93

Query: 74 GYPVAYVA 82
          G    Y+A
Sbjct: 94 GVSKNYLA 97

The following BLAST results are available for this feature:

BLAST of CsGy5G009647 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WT8	3.7e-06	38.20	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=...	[more]

BLAST of CsGy5G009647 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8648095.1	9.08e-71	100.00	hypothetical protein Csa_004720 [Cucumis sativus]	[more]
KAE8648094.1	3.69e-52	74.53	hypothetical protein Csa_004738 [Cucumis sativus]	[more]
XP_010434812.1	8.48e-09	40.45	PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa]	[more]
XP_010449778.1	1.20e-08	41.57	PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa]	[more]
KAF8093897.1	2.23e-08	46.77	hypothetical protein N665_0375s0014 [Sinapis alba]	[more]

BLAST of CsGy5G009647 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J0MCI3	1.08e-08	47.54	cysteine proteinase inhibitor 4-like OS=Raphanus sativus OX=3726 GN=LOC108840944...	[more]
A0A068VPR8	1.12e-07	49.09	Cystatin domain-containing protein OS=Coffea canephora OX=49390 GN=GSCOC_T000086...	[more]
A0A6P6XFN5	1.12e-07	49.09	cysteine proteinase inhibitor-like OS=Coffea arabica OX=13443 GN=LOC113742174 PE...	[more]
A0A6J1EK72	1.12e-07	38.55	uncharacterized protein LOC111433339 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A484MG82	1.46e-07	40.23	Cystatin domain-containing protein OS=Cuscuta campestris OX=132261 GN=CCAM_LOCUS...	[more]

BLAST of CsGy5G009647 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16500.1	2.7e-07	38.20	Cystatin/monellin superfamily protein	[more]
AT5G47550.1	2.7e-04	38.24	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 14..101 e-value: 3.0E-13 score: 49.9
None	No IPR available	GENE3D	3.10.450.10		coord: 6..104 e-value: 5.0E-13 score: 50.9
None	No IPR available	PANTHER	PTHR47116:SF5	PHLOEM FILAMENT PROTEIN	coord: 9..104
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 19..101
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 9..104

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy5G009647.1	CsGy5G009647.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005773	vacuole
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity