CsGy5G005000 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G005000
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionMannose-6-phosphate isomerase
LocationGy14Chr5: 3208431 .. 3211254 (-)
RNA-Seq ExpressionCsGy5G005000
SyntenyCsGy5G005000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGAGGAAGATGATAAGAAATTGAGGAAGTGTAGGAAGAAGAGACTGTTTGGCGCCGACAGGAGGAAGCGGCTCCTTCGTCTAAAATGCTGTGTTCAAAACTACGATTGGGGAGTTATTGGGTGTAATTCCCAAGTTGCGAGGCTGTTTTTTCTCAATTCAGGTCGCTGCAATGTCGATCCGGGGGAATGTTACGCAGAATTCTGGATTGGGACGCATAAATCTGGTCCGTCTTTTGTTGTTTATGGACGGGATAATAATGCTGTCGCGGCGTTTGGATCCAAACCCCTGAGTCTTAAGGATTGTATCTCCGTCGATCCGGTTGCTGTGCTTGGTGATAAGGTTGCGAGGAAATGGGGAGGCGATCTTCCGTTTCTTTTCAAGGTCGGCTTTATTTTCCTTTAGATCCTTGGGGAATGTGTTTTGTCGGTGGGCTTGATTTCTTTCAAGATTTCACAATCGGACACGAATTTTGGGTTTTAATTATGCTCTGCATTGTGTCGGTGTGTGAATTAATTTGGATGCTGAGCATTTGCCTGTTGAATGACATTAATGCTTCATTACCGAAAATTGTTTGCTGCAGCTCTTTGAATTGTATGTTCAATTAGTTCTCGAACCTCAATTACTGTTCTTATTTTCCTTGATGTTAATGTTTTGAGTTCAGTTGGACTGCAACTTGATGATTTCTTATTGGGAGCATAGGTGCTGTCGATAGAGAAGGCTTTATCTATTCAGGCTCATCCAGATAAAGATTTGGCGAGATCCTTGAACGAAGCTCAACCAAGCATCTATAAAGATGATAACCACAAGCCTGAAATGGCTCTAGCATTGACAAGATTTGAAGCCCTCTGTGGGTTCATCAGCTCTAAGGTACCATAACATATAGTACCAGAGACTTACTCTTCTGGGGTTTTCAAAAAGTTTCTTAAACTTACTTTAAATATCTTAATGATTCTTACTCTGTTCTTATTACACTAATTGGATTTAGAGAGATGAAAGAAGCTCAAATCAAGATGTTAATTCCGCTTTTTGGTTAATCGTGTATGTATTTCCAGATGGCTGAAAATTCAGAAAAATAAACAAAGACTATTAGTAACTGATAATGAATGAAGTTTCTTTATAGATTCGTTTACATTGCAAAACCCATGTCATTTGATTTTACTTATTTCATTGTTTTTCAGGAGCTCAAGGCGGTGCTTAGTAGCGTTCCTGAGATAGTAGAATTGGTCCAATGTGCTGATGCAGAGAAGTTCTCTCACGATAGTGAGCAAGATGGGAAGGAGAAAGTAAAACAACTTTTTGAGTCCATATTTAGTCAGATCATGTCATCTAACAAGGGCATAGTTCGCGAGGCTGTATGTAAACTAAAAAGACGTCTTAGTTTGGAGAAAAAGGTAATTGAAGTTTTATTTTATATCTCTACAAATGATCTTGTCAGCAGAATTTGGCATTTTTCAGTGGCGACTTTTTTTTTTTAAATCTCAAGGGTTACCATCGATAGGAAGTTCTGTGATCAATAGGATATATTTCATAATAAAGCTTAAATTGTTCTTTCACTTCATCAATCTCTGCAAAATATTATTCATTTGTTTTCGTATAGTTTTATGCAACTACTCATTGTATTACCTTCTTTTTCTTTCATCAGAAGAGACAATTATCTGCCAAAGAACAATTGATTCTCAGACTAGAAAGTCAATATCCTGCTGATGTTGGTATACTAGCAGCATATTTTCTCAACTATGTGGAGTTAAAACCAGGCGAAGCATTATATGTTGGTCCAAATGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCAGACAATGTTGTTCGTGCAGGCCTTACCTCTAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGTAATCACATATTGTTTGCAGGAGCTGGTTTTTATAGATGTGATTCTAATATGTAAACTGATTATATGCTATTATTGGTTCTTGTTTTCCATGTCCTCTTTCTTGGAGTCTAAAGGCTTATTCAAATTAATTAATTTGCATTTTTTTTTAGTTTCGATGTTAGTCTTAAATGCACATTATATATGTTGTCAAGCTTTCATATTGGTTGGAGCTCTTAGAGTCTATTATGACTTGATGCAAACATACATCTCTCTTGACCAATTGGAGAAATCTTTTTATAACTCCTTTGGTTTGGGCATTTTTCCCTTCTTTTATAAATTTCATATATCGATATTTATATATTTGTTGGAGCTCGCTTATATTTCCAGATGAGTATCTTTTTTTTCCTTTTTCTAGTGCTTGCTAACAGAAAGTAATTAAAAATATGCCTCGGTAGTACGATCTCAAACGACTTGGTTAATGATTCATATTGATCTTCCATTATTACTCATTGAAGATAAGGGAAATAGTTGAAATCTTTCCTCTTTGTGTTTTTGCCTTGGAGTTTCATTGAGAGAAGATTGCTTATAATCAGTTTCTTCATCACAGGGTTTTCCTGAAATTTTGAGAGGAGTTTCCTTGAATGAATACACAAGAAAATACGCCCCTCCATTCGATGAATTTGAAGTCGATCGCTGTATTCTCCCACAAGCTGCATCCGTGTCGTTCCCATCCGTCCCAGGACCATCTCTGTTTCTAGTGATGTCCGGGAAAGGGACAATTATCACAGGCTATTCCGAAGAAACTACATTGCAAGAAGGTGAAGTTCTGTTCGTTCCAGCATATATGGAGGTGAGTATAACAGCTACAGCTATAGAATTACATATGTACAGAGCTGGGATCAACAACAGATTCTTTCGAGACTTGTGA

mRNA sequence

ATGGACGAGGAAGATGATAAGAAATTGAGGAAGTGTAGGAAGAAGAGACTGTTTGGCGCCGACAGGAGGAAGCGGCTCCTTCGTCTAAAATGCTGTGTTCAAAACTACGATTGGGGAGTTATTGGGTGTAATTCCCAAGTTGCGAGGCTGTTTTTTCTCAATTCAGGTCGCTGCAATGTCGATCCGGGGGAATGTTACGCAGAATTCTGGATTGGGACGCATAAATCTGGTCCGTCTTTTGTTGTTTATGGACGGGATAATAATGCTGTCGCGGCGTTTGGATCCAAACCCCTGAGTCTTAAGGATTGTATCTCCGTCGATCCGGTTGCTGTGCTTGGTGATAAGGTTGCGAGGAAATGGGGAGGCGATCTTCCGTTTCTTTTCAAGGTGCTGTCGATAGAGAAGGCTTTATCTATTCAGGCTCATCCAGATAAAGATTTGGCGAGATCCTTGAACGAAGCTCAACCAAGCATCTATAAAGATGATAACCACAAGCCTGAAATGGCTCTAGCATTGACAAGATTTGAAGCCCTCTGTGGGTTCATCAGCTCTAAGGAGCTCAAGGCGGTGCTTAGTAGCGTTCCTGAGATAGTAGAATTGGTCCAATGTGCTGATGCAGAGAAGTTCTCTCACGATAGTGAGCAAGATGGGAAGGAGAAAGTAAAACAACTTTTTGAGTCCATATTTAGTCAGATCATGTCATCTAACAAGGGCATAGTTCGCGAGGCTGTATGTAAACTAAAAAGACGTCTTAGTTTGGAGAAAAAGAAGAGACAATTATCTGCCAAAGAACAATTGATTCTCAGACTAGAAAGTCAATATCCTGCTGATGTTGGTATACTAGCAGCATATTTTCTCAACTATGTGGAGTTAAAACCAGGCGAAGCATTATATGTTGGTCCAAATGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCAGACAATGTTGTTCGTGCAGGCCTTACCTCTAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGGTTTTCCTGAAATTTTGAGAGGAGTTTCCTTGAATGAATACACAAGAAAATACGCCCCTCCATTCGATGAATTTGAAGTCGATCGCTGTATTCTCCCACAAGCTGCATCCGTGTCGTTCCCATCCGTCCCAGGACCATCTCTGTTTCTAGTGATGTCCGGGAAAGGGACAATTATCACAGGCTATTCCGAAGAAACTACATTGCAAGAAGGTGAAGTTCTGTTCGTTCCAGCATATATGGAGGTGAGTATAACAGCTACAGCTATAGAATTACATATGTACAGAGCTGGGATCAACAACAGATTCTTTCGAGACTTGTGA

Coding sequence (CDS)

ATGGACGAGGAAGATGATAAGAAATTGAGGAAGTGTAGGAAGAAGAGACTGTTTGGCGCCGACAGGAGGAAGCGGCTCCTTCGTCTAAAATGCTGTGTTCAAAACTACGATTGGGGAGTTATTGGGTGTAATTCCCAAGTTGCGAGGCTGTTTTTTCTCAATTCAGGTCGCTGCAATGTCGATCCGGGGGAATGTTACGCAGAATTCTGGATTGGGACGCATAAATCTGGTCCGTCTTTTGTTGTTTATGGACGGGATAATAATGCTGTCGCGGCGTTTGGATCCAAACCCCTGAGTCTTAAGGATTGTATCTCCGTCGATCCGGTTGCTGTGCTTGGTGATAAGGTTGCGAGGAAATGGGGAGGCGATCTTCCGTTTCTTTTCAAGGTGCTGTCGATAGAGAAGGCTTTATCTATTCAGGCTCATCCAGATAAAGATTTGGCGAGATCCTTGAACGAAGCTCAACCAAGCATCTATAAAGATGATAACCACAAGCCTGAAATGGCTCTAGCATTGACAAGATTTGAAGCCCTCTGTGGGTTCATCAGCTCTAAGGAGCTCAAGGCGGTGCTTAGTAGCGTTCCTGAGATAGTAGAATTGGTCCAATGTGCTGATGCAGAGAAGTTCTCTCACGATAGTGAGCAAGATGGGAAGGAGAAAGTAAAACAACTTTTTGAGTCCATATTTAGTCAGATCATGTCATCTAACAAGGGCATAGTTCGCGAGGCTGTATGTAAACTAAAAAGACGTCTTAGTTTGGAGAAAAAGAAGAGACAATTATCTGCCAAAGAACAATTGATTCTCAGACTAGAAAGTCAATATCCTGCTGATGTTGGTATACTAGCAGCATATTTTCTCAACTATGTGGAGTTAAAACCAGGCGAAGCATTATATGTTGGTCCAAATGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCAGACAATGTTGTTCGTGCAGGCCTTACCTCTAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGGTTTTCCTGAAATTTTGAGAGGAGTTTCCTTGAATGAATACACAAGAAAATACGCCCCTCCATTCGATGAATTTGAAGTCGATCGCTGTATTCTCCCACAAGCTGCATCCGTGTCGTTCCCATCCGTCCCAGGACCATCTCTGTTTCTAGTGATGTCCGGGAAAGGGACAATTATCACAGGCTATTCCGAAGAAACTACATTGCAAGAAGGTGAAGTTCTGTTCGTTCCAGCATATATGGAGGTGAGTATAACAGCTACAGCTATAGAATTACATATGTACAGAGCTGGGATCAACAACAGATTCTTTCGAGACTTGTGA

Protein sequence

MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNVDPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCGFISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIVREAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVGPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYTRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLFVPAYMEVSITATAIELHMYRAGINNRFFRDL*
Homology
BLAST of CsGy5G005000 vs. ExPASy Swiss-Prot
Match: Q9M884 (Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.0e-125
Identity = 233/428 (54.44%), Postives = 315/428 (73.60%), Query Frame = 0

Query: 20  ADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNVDPGECYAEFWIGTHKSGPS 79
           ADRR RL RL+C V++YDWG IG +S V R++  NS    +DP   YAE W+GTH+SGPS
Sbjct: 15  ADRR-RLRRLRCSVKDYDWGKIGSDSLVYRVYAANSD-YEIDPTRPYAELWMGTHESGPS 74

Query: 80  FVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKWGGDLPFLFKVLSIEKALSI 139
           ++          A GS  ++L+  I+ +P + LG++V  KWG DLPFLFKVLS+ + LSI
Sbjct: 75  YL--------EDADGSNGVTLRSWITENPKS-LGNRVLEKWGCDLPFLFKVLSVARPLSI 134

Query: 140 QAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCGFISSKELKAVLSSVPEIVE 199
           QAHPDK LA+ +++A P++YKDDNHKPEMALA T+FEALCGFI  +ELK+V+ ++PEI E
Sbjct: 135 QAHPDKKLAKKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEE 194

Query: 200 LVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIVREAVCKLKRRLSLEKKKRQ 259
           LV   +A +    +E D +EKVK +  +IF+ +MS++    ++ V KLKRRL +E ++RQ
Sbjct: 195 LVGSEEANQVFCITEHD-EEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQ 254

Query: 260 LSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVGPNEPHAYISGECIECMATS 319
           L+ KE+L+L+LE QYP D+G+++A+F NYV+L PGEALY+G NEPHAY+ GEC+E MATS
Sbjct: 255 LTDKERLVLKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATS 314

Query: 320 DNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYTRKYAPPFDEFEVDRCILPQ 379
           DNVVRAGLTSK  D+Q L SML+YK G+PEIL+G  +  Y  +Y PPF+EFEVD C LP 
Sbjct: 315 DNVVRAGLTSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPS 374

Query: 380 AASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLFVPAYMEVSITATAIELHMY 439
            AS  FPSVPGPSL LV+ G+G + T  S +  +  G+VLFVPA  E+ + +++ +L +Y
Sbjct: 375 GASTVFPSVPGPSLLLVLQGEGRMSTEASAD-GISMGDVLFVPADTEIHLRSSS-DLKLY 428

Query: 440 RAGINNRF 448
           RAGIN+RF
Sbjct: 435 RAGINSRF 428

BLAST of CsGy5G005000 vs. ExPASy Swiss-Prot
Match: Q9FZH5 (Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 2.6e-125
Identity = 226/428 (52.80%), Postives = 305/428 (71.26%), Query Frame = 0

Query: 24  KRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNVDPGECYAEFWIGTHKSGPSFVVY 83
           + + RL+C V+NY+WG +G  S VARL   N+G+  VD    YAEFW+GTH+SGPS V +
Sbjct: 20  EEIQRLRCFVKNYEWGKLGPESLVARLQEANTGQ-RVDSEIPYAEFWMGTHESGPSHVEF 79

Query: 84  GRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHP 143
           G  +          ++LK  + +D   +LG KV  KWG DLPFLFKVLS+ KALSIQAHP
Sbjct: 80  GSGH---GVSDKCMVTLKSWV-LDNPNLLGSKVVDKWGCDLPFLFKVLSVTKALSIQAHP 139

Query: 144 DKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCGFISSKELKAVLSSVPEIVELVQC 203
           +K LA  L+   P +Y+D+NHKPE+ALA+T F+ALCGF++ KELK V+++VPEI ELV  
Sbjct: 140 NKALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVGS 199

Query: 204 ADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIVREAVCKLKRRLSLEKKKRQLSAK 263
             A++  +  E D  E++K +   IF+Q+MS++    ++ V ++K RL LE K R+LS K
Sbjct: 200 KAADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSEK 259

Query: 264 EQLILRLESQYPADVGILAAYFLNYVELKPGEALYVGPNEPHAYISGECIECMATSDNVV 323
           E+L+L LE QY  D+G+++A+F NYV+L PGEALY+  NEPHAYISG+C+ECMA SDNVV
Sbjct: 260 EKLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNVV 319

Query: 324 RAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYTRKYAPPFDEFEVDRCILPQAASV 383
           RAGLT K RDVQ L SML YK G+PEIL+G  L  Y  +Y PPFDEFEVD C LP+  S 
Sbjct: 320 RAGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKST 379

Query: 384 SFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLFVPAYMEVSITATAIELHMYRAGI 443
            FP+VPGPS++LV+ GKG + TG S +  +  G+VLFVPA +E+ +T  +  + +YRAG+
Sbjct: 380 VFPAVPGPSVYLVIEGKGQLRTG-SSKVLVNRGDVLFVPADIEIHVTGESDVMKLYRAGV 439

Query: 444 NNRFFRDL 452
           ++RFF+ L
Sbjct: 440 SSRFFQTL 441

BLAST of CsGy5G005000 vs. ExPASy Swiss-Prot
Match: Q54PA0 (Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=mpi PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.7e-76
Identity = 175/444 (39.41%), Postives = 256/444 (57.66%), Query Frame = 0

Query: 21  DRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCN--VDPGECYAEFWIGTHKSGP 80
           +    ++ LKC  QNY+WG  G NS VA+L    +  C+  +     YAE W+G H S P
Sbjct: 16  NNNNNIVLLKCVSQNYEWGKYGSNSTVAKLLKGYAKECSDIIKETIPYAELWMGDHVSAP 75

Query: 81  SFVVYGRDNNAVAAF---GSKPLSLKDCI-SVDPVAVLGDKVARKWGGDLPFLFKVLSIE 140
           S V Y      +  +     K ++ K    S    ++ G+ V +++G D PFLFKVLSI 
Sbjct: 76  SKVEYKNKELKLREYIDTVQKEINEKSSTSSSSSSSIRGEIVEKRFGNDFPFLFKVLSIR 135

Query: 141 KALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCGFISSKELKAVLSSV 200
            ALSIQAHPD  LA+ L +  P+IYKD  HKPE+A+A T FEALC F    E+++ + ++
Sbjct: 136 TALSIQAHPDSQLAQVLFKKYPNIYKDPYHKPEIAIATTPFEALCSFRPLSEIQSFIDTI 195

Query: 201 PEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIVREAVCKLKRRLSLE 260
           PE           K S  +     +  K+  +SI + ++ ++  ++   + +L  RL+ E
Sbjct: 196 PEF----------KNSLPNNLIKLDDCKEYLKSIVTSLLKADGLLISNNLKELNNRLN-E 255

Query: 261 KKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVGPNEPHAYISGECIE 320
           K++ +    ++L+L+L SQYP DVG+  AY LNY+ LKPGEAL++G  EPHAYISG+C+E
Sbjct: 256 KREEERDDLDRLVLKLYSQYPGDVGVFFAYILNYIVLKPGEALFLGAGEPHAYISGDCVE 315

Query: 321 CMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL-----RGVSLNEYTRKYAPPFDE 380
           CMA SDNVVRAGLT K +DV  L  ML Y+ G P+++       +SL  Y R Y PP DE
Sbjct: 316 CMAPSDNVVRAGLTPKLKDVDTLGDMLTYRTGRPDLVVPQQRPDLSLTNY-RCYQPPVDE 375

Query: 381 FEVDRCILPQAA-SVSFPSVPGPSLFLVMSGKGTIITGYSEET--TLQEGEVLFVPAYME 440
           F+V+   L     +++  S  GPS+ LV SG  +I    ++ T   L  G +LFVPA  +
Sbjct: 376 FQVEYYHLDDCCKNINVYSSKGPSIVLVYSGNLSIENKSNQSTLNNLHTGSILFVPANTD 435

Query: 441 VSI--TATAIELHMYRAGINNRFF 449
                + ++I + +Y A ++NR F
Sbjct: 436 YQFIQSDSSIPVSIYVASVSNRIF 447

BLAST of CsGy5G005000 vs. ExPASy Swiss-Prot
Match: Q9HFU4 (Mannose-6-phosphate isomerase OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=MAN1 PE=3 SV=2)

HSP 1 Score: 272.7 bits (696), Expect = 7.4e-72
Identity = 174/435 (40.00%), Postives = 238/435 (54.71%), Query Frame = 0

Query: 33  VQNYDWGVIGCNSQVARLFFLNSGRCNVDPGECYAEFWIGTHKSGPSFVVYGRDNNAVAA 92
           + +YDWG  G  S  A+L   +    ++D  + YAE W+GTH + PS +    DN     
Sbjct: 12  INSYDWGKKGSASLAAQLATTSIPDFSIDEDKAYAELWMGTHPNNPSRL---SDNTL--- 71

Query: 93  FGSKPLSLKDCISVDPVAVLGDKVARKW----GGDLPFLFKVLSIEKALSIQAHPDKDLA 152
                  L + +   P  ++G  V+ K+     G LPFLFKVLSI  ALSIQAHPDK LA
Sbjct: 72  -------LSEHLKSHP-ELIGSSVSSKFEDCKDGSLPFLFKVLSIGTALSIQAHPDKPLA 131

Query: 153 RSLNEAQPSIYKDDNHKPEMALALTRFEALCGFISSKELKAVLSSVPEIVELVQCADAEK 212
           + L + +P +YKD NHKPEMA+ALT F A   F+    L   L +VPE+ E V  +  E 
Sbjct: 132 KKLFDEKPDVYKDPNHKPEMAIALTPFLAFLNFLPLSVLLLHLLTVPELQEFVDSSLTES 191

Query: 213 FSHD----------------SEQDGKEKVKQLFESIFSQIMSSNKGIVREAVCKLKRRLS 272
            +                  +E     + K + + IF+ +MS++K +V EA+ KL +R  
Sbjct: 192 LASSLGLPTSQPPDTSLFKPTESPATAEQKDILKQIFAALMSADKKLVEEAISKLIKRYQ 251

Query: 273 LEKK-KRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVGPNEPHAYISGE 332
            ++  K    +   L LRL  QYP DVG+L  + LN VELK GEA ++G NEPHAYI G+
Sbjct: 252 AKRDIKENEKSLVDLALRLNDQYPGDVGVLCVFLLNVVELKRGEAAFLGANEPHAYIEGD 311

Query: 333 CIECMATSDNVVRAGLTSKKRDVQALLSMLNYK--QGFPEILRGVSL---NEYTRKYAPP 392
            IECMATSDNVVRAGLT K RDV  L+SML Y+   G  ++L+       ++ T+ Y PP
Sbjct: 312 IIECMATSDNVVRAGLTPKLRDVDTLVSMLTYEAAPGNKQLLQPTPFQKGDDTTKLYDPP 371

Query: 393 FDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLFVPAYME 442
             EF V R  L +    S   V GPSL ++  G+G +  G ++ +    G+V+FV A  E
Sbjct: 372 IAEFSVLRTELSKGMKTSHRPVEGPSLCVITEGEGVVRNG-NDRSEFVRGDVIFVGAGKE 430

BLAST of CsGy5G005000 vs. ExPASy Swiss-Prot
Match: Q9GP38 (Probable mannose-6-phosphate isomerase OS=Echinococcus multilocularis OX=6211 GN=PMIH PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 3.1e-70
Identity = 173/449 (38.53%), Postives = 242/449 (53.90%), Query Frame = 0

Query: 26  LLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNVDPGECYAEFWIGTHKSGPSFVVYGR 85
           +LRLKC  Q YDWG +G NS V +L   +           YAE W+GTH SG S      
Sbjct: 1   MLRLKCASQRYDWGKVGVNSIVYKLQVASENPDISHVDLPYAELWMGTHPSGSS------ 60

Query: 86  DNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHPDK 145
                  + +  ++L   I  +P A LG      +G  LPFLFKVLS+ KALSIQAHPDK
Sbjct: 61  -----CLWDNPHVTLAAHIQENP-ASLGKPSLIYFGRRLPFLFKVLSVAKALSIQAHPDK 120

Query: 146 DLARSLNEAQPSIYKDDNHKPEMALALTRFEALCGFISSKELKAVLSSVPEIVEL----- 205
            +A  L+  QP +YKD +HKPEMA+ALT FEAL GF    ++ A + + PE+ EL     
Sbjct: 121 KMAVRLHAEQPDLYKDGSHKPEMAIALTDFEALLGFRPLNQILAFIQAFPELAELTTLEL 180

Query: 206 --------VQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIVREAVCKLKRRLS 265
                   VQ    ++   +  +   EKV+   +S+ ++  + +K +    +      L 
Sbjct: 181 PSPEEKRDVQPPSIKQLYSNLMRSSPEKVESTIKSLLNRFTTGSKSVCDPPL----SILG 240

Query: 266 LEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVGPNEPHAYISGEC 325
           ++ ++  + A   L LRL   +P DVG L+ +FLNY+ LK GEA+++  N PHAY+SG+C
Sbjct: 241 VDLQEEDVQALVDLFLRLTQAFPGDVGCLSIFFLNYIRLKSGEAIFLKANTPHAYLSGDC 300

Query: 326 IECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT------RKYAPP 385
           +ECMA SDNVVRAGLT K +DV+ LL ML+Y      +  G +    T      + + PP
Sbjct: 301 VECMANSDNVVRAGLTPKFKDVERLLEMLDYTPLTDSLRLGATQPIPTPEGISMKSFIPP 360

Query: 386 FDEFEVDRCIL-PQAASVSFPSVPGPSLFLVMSGKGTII---TG--YSEETTLQEGEVLF 445
             EF VD      ++   S PSVP  S+ L + G+GTI    TG   S+ET    G V F
Sbjct: 361 VSEFAVDVIQFDAESRGFSLPSVPTASILLFLHGQGTITCPSTGEACSQETKFGPGFVYF 420

Query: 446 VPAYMEVSITATAI---ELHMYRAGINNR 447
           VPA M V++ +       LH +RA +N +
Sbjct: 421 VPADMIVNLVSKEDRKGSLHAFRAYVNRK 433

BLAST of CsGy5G005000 vs. NCBI nr
Match: XP_004143675.1 (mannose-6-phosphate isomerase 1 [Cucumis sativus] >XP_011654856.1 mannose-6-phosphate isomerase 1 [Cucumis sativus] >KGN50328.1 hypothetical protein Csa_000669 [Cucumis sativus])

HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 0

Query: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60
           MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV
Sbjct: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60

Query: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120
           DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW
Sbjct: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180
           GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG
Sbjct: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV
Sbjct: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240

Query: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300
           REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG
Sbjct: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420
           RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420

Query: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451
           VPAYMEVSITATAIELHMYRAGINNRFFRDL
Sbjct: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451

BLAST of CsGy5G005000 vs. NCBI nr
Match: XP_038875726.1 (mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875727.1 mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875728.1 mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875729.1 mannose-6-phosphate isomerase 1-like [Benincasa hispida])

HSP 1 Score: 847 bits (2189), Expect = 4.09e-309
Identity = 425/451 (94.24%), Postives = 436/451 (96.67%), Query Frame = 0

Query: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60
           MDEEDD KL+KCRKKRL GADRRKRLLRLKCCVQNYDWGVIGCNSQVARLF LNSG C++
Sbjct: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60

Query: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120
           DP ECYAEFWIGTHKSGPSFVV+GRDNNA AAFGSKPLSLKD ISVDP AVLGDKVARKW
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180
           GGDLPFLFKVLSIEKALSIQAHPDKDLARSLN+AQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELK VLSSVPEIVELVQCADAEKFSH+SEQDGKEKVKQLFESIFSQIMSSNKGIV
Sbjct: 181 FISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIV 240

Query: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300
           REA+CKLKRRLSLEKKKRQLSAKEQLILRLE QYPADVGILAA+FLNYVELKPGEALYVG
Sbjct: 241 REAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420
           RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTI+TGYSEE T QEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLF 420

Query: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451
           VPAYMEVSITA AIELHMYRAG+NNRFF DL
Sbjct: 421 VPAYMEVSITAAAIELHMYRAGVNNRFFGDL 451

BLAST of CsGy5G005000 vs. NCBI nr
Match: XP_023511752.1 (mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 802 bits (2071), Expect = 3.76e-291
Identity = 400/451 (88.69%), Postives = 427/451 (94.68%), Query Frame = 0

Query: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60
           M EEDDK+L+K +KKRL GADRR+RL+RLKCCVQNYDWGV+GCNSQVAR+F LNSG  + 
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRRRLVRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120
           DP ECYAEFWIGTHKSGPSFV +GRD NA AA GSKPLSLKD I+VDP AVLGDKVARKW
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNA-AAIGSKPLSLKDWITVDPGAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180
           GGDLPFLFKVLS+EKALSIQAHPDK+LAR+L+EAQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELK VLSSVPEIVELVQ ADAEKFSHDSEQ+GKEKVK LFESIFSQIMSSNK +V
Sbjct: 181 FISSKELKGVLSSVPEIVELVQVADAEKFSHDSEQNGKEKVKSLFESIFSQIMSSNKAMV 240

Query: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300
           REA+CKLKRRLSLEKKKRQLS KEQL+LRLE+QYPADVGILAA+FLNYV+LKPGEALY+G
Sbjct: 241 REAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420
           RKYAPPFDEFEVDRCILP AASVSFPSVPGPSLFL+MSGKGTI+TGYSEETT QEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEETTFQEGEVLF 420

Query: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451
           VPAYMEVS+TA A ELHMYRAG+NNRFFRDL
Sbjct: 421 VPAYMEVSVTAAATELHMYRAGVNNRFFRDL 450

BLAST of CsGy5G005000 vs. NCBI nr
Match: XP_022921753.1 (mannose-6-phosphate isomerase 1-like [Cucurbita moschata])

HSP 1 Score: 800 bits (2067), Expect = 1.53e-290
Identity = 400/451 (88.69%), Postives = 427/451 (94.68%), Query Frame = 0

Query: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60
           M EEDDK+L+K +KKRL GADRRKRL+RLKCCVQNYDWGV+GCNSQVAR+F LNSG  + 
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRKRLVRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120
           DP ECYAEFWIGTHKSGPSFV +GRD NA AA GSKPLSLKD I+VDP AVLGDKVARKW
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNA-AAIGSKPLSLKDWITVDPGAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180
           GGDLPFLFKVLS+EKALSIQAHPDK+LAR+L+EAQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELK VLSSVPEIVELVQ ADAEKFSHDSEQ+GKEKVK LFESIFSQIMSSNK +V
Sbjct: 181 FISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFSQIMSSNKAMV 240

Query: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300
           REA+CKLKRRLSLEKKKRQLSAKEQL+LRLE+QYPADVGILAA+FLNYV+LKPGEALY+G
Sbjct: 241 REAICKLKRRLSLEKKKRQLSAKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420
           RKYAPPFDEFEVDRCILP AASVSFPSVPGPSLFL+MSGKGTI+TGYSEE T QEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEEITFQEGEVLF 420

Query: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451
           VPAYMEVS+TA A ELHMYRAG+NNRFF+DL
Sbjct: 421 VPAYMEVSVTAAATELHMYRAGVNNRFFKDL 450

BLAST of CsGy5G005000 vs. NCBI nr
Match: XP_023549674.1 (mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 800 bits (2066), Expect = 2.34e-290
Identity = 405/452 (89.60%), Postives = 425/452 (94.03%), Query Frame = 0

Query: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60
           M+EEDD +L+K +KKRL GADRRKRLLRLKC VQNYDWGVIGC+SQVARLF LNSG    
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60

Query: 61  DPGECYAEFWIGTHKSGPSFVVYGRD-NNAVAAFGSKPLSLKDCISVDPVAVLGDKVARK 120
           DP ECYAEFWIGTHKSGPSFVV+GRD NNA AA GS  +SLKD ISVDP AVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALC 180
           WGGDLPFLFKVLS+EKALSIQAHPDK+LAR+L+EAQPSIYKDDNHKPEMALALT FEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELK VLSSVPEIVELVQC DAEKFSHDS QDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYV 300
           VREA+CKLKRRLS EKKKRQLSAKEQLILRLE QYPADVGILA +FLNYV+LKPGEALYV
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLV+SGKGTIITGYSEE+TL+EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420

Query: 421 FVPAYMEVSITATAIELHMYRAGINNRFFRDL 451
           FVPAYMEVSIT+ A ELHMYRAG+NNRFF DL
Sbjct: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452

BLAST of CsGy5G005000 vs. ExPASy TrEMBL
Match: A0A0A0KL16 (Mannose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_5G167200 PE=3 SV=1)

HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 0

Query: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60
           MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV
Sbjct: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60

Query: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120
           DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW
Sbjct: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180
           GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG
Sbjct: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV
Sbjct: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240

Query: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300
           REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG
Sbjct: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420
           RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420

Query: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451
           VPAYMEVSITATAIELHMYRAGINNRFFRDL
Sbjct: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451

BLAST of CsGy5G005000 vs. ExPASy TrEMBL
Match: A0A6J1E4R0 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111429906 PE=3 SV=1)

HSP 1 Score: 800 bits (2067), Expect = 7.40e-291
Identity = 400/451 (88.69%), Postives = 427/451 (94.68%), Query Frame = 0

Query: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60
           M EEDDK+L+K +KKRL GADRRKRL+RLKCCVQNYDWGV+GCNSQVAR+F LNSG  + 
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRKRLVRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120
           DP ECYAEFWIGTHKSGPSFV +GRD NA AA GSKPLSLKD I+VDP AVLGDKVARKW
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNA-AAIGSKPLSLKDWITVDPGAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180
           GGDLPFLFKVLS+EKALSIQAHPDK+LAR+L+EAQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELK VLSSVPEIVELVQ ADAEKFSHDSEQ+GKEKVK LFESIFSQIMSSNK +V
Sbjct: 181 FISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFSQIMSSNKAMV 240

Query: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300
           REA+CKLKRRLSLEKKKRQLSAKEQL+LRLE+QYPADVGILAA+FLNYV+LKPGEALY+G
Sbjct: 241 REAICKLKRRLSLEKKKRQLSAKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420
           RKYAPPFDEFEVDRCILP AASVSFPSVPGPSLFL+MSGKGTI+TGYSEE T QEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEEITFQEGEVLF 420

Query: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451
           VPAYMEVS+TA A ELHMYRAG+NNRFF+DL
Sbjct: 421 VPAYMEVSVTAAATELHMYRAGVNNRFFKDL 450

BLAST of CsGy5G005000 vs. ExPASy TrEMBL
Match: A0A6J1I443 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111470445 PE=3 SV=1)

HSP 1 Score: 799 bits (2064), Expect = 2.12e-290
Identity = 399/451 (88.47%), Postives = 426/451 (94.46%), Query Frame = 0

Query: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60
           M EEDDK+L+K +KKRL GADRR+RLLRLKCCVQNYDWGV+GCNSQVAR+F LNSG  + 
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120
           DP ECYAEFWIGTHKSGPSFV +GRD NA AA GSKPLSLKD I+VDP AVLGDKVARKW
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNA-AAIGSKPLSLKDWITVDPGAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180
           GGDLPFLFKVLS+EKALSIQAHPDK+LAR+L+EAQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELK VLSSVPEIVELVQ ADAEKFSHDSEQ+GKEKVK LFESIF QIMSSNK +V
Sbjct: 181 FISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMV 240

Query: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300
           REA+CKLKRRLSLEKKKRQLS KEQL+LRLE+QYPADVGILAA+FLNYV+LKPGEALY+G
Sbjct: 241 REAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420
           RKYAPPFDEFEVDRCILP AASVSFPSVPGPSLFL+MSGKGTI+TGYSEE+T QEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLF 420

Query: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451
           VPAYMEVS+TA A ELHMYRAG+NNRFFRDL
Sbjct: 421 VPAYMEVSVTAAATELHMYRAGVNNRFFRDL 450

BLAST of CsGy5G005000 vs. ExPASy TrEMBL
Match: A0A6J1FJN3 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111444656 PE=3 SV=1)

HSP 1 Score: 797 bits (2059), Expect = 1.32e-289
Identity = 403/452 (89.16%), Postives = 424/452 (93.81%), Query Frame = 0

Query: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60
           M+EEDD +L+K +KKRL GADRRKRLLRLKC VQNYDWGVIGCNSQVARLF LNSG    
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPGECYAEFWIGTHKSGPSFVVYGRD-NNAVAAFGSKPLSLKDCISVDPVAVLGDKVARK 120
           DP ECYAEFWIGTHKSGPSFVV+GRD NNA AA GS  +SLKD ISVDP AVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALC 180
           WGGDLPFLFKVLS+EKALSIQAHPDK+LAR+L+EAQPSIY+DDNHKPEMALALT FEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYEDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELK VLSSVPEIVELVQC D EKFSHDS QDGKEKVKQLFESIFSQIMSSNK I
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDTEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKAI 240

Query: 241 VREAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYV 300
           VREA+CKLKRRLS EKKKRQLSAKEQLILRLE QYPADVGILAA+FLNYV+LKPGEALYV
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRD+QALLSMLNYKQGFPEIL+GVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDIQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLV+SGKGTIITGYSEE+TLQEGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITATAIELHMYRAGINNRFFRDL 451
           FVPAYMEVSIT+ A ELH+YRAG+NNRFF DL
Sbjct: 421 FVPAYMEVSITSGATELHVYRAGVNNRFFGDL 452

BLAST of CsGy5G005000 vs. ExPASy TrEMBL
Match: A0A6J1K047 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111489838 PE=3 SV=1)

HSP 1 Score: 793 bits (2048), Expect = 6.25e-288
Identity = 402/451 (89.14%), Postives = 423/451 (93.79%), Query Frame = 0

Query: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60
           M+EEDD +L+K +KKRL GADRRKRLLRLKC VQNYDWGVIGCNSQVARLF LNSG    
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPGECYAEFWIGTHKSGPSFVVYGRD-NNAVAAFGSKPLSLKDCISVDPVAVLGDKVARK 120
           DP ECYAEFWIGTHKSGPSFVV+GRD NNA AA GS  +SLKD ISVDP AVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALC 180
           WGG+LPFLFKVLS+EKALSIQAHPDK+LAR+L+EAQPSIYKDDNHKPEMALALT FEALC
Sbjct: 121 WGGELPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELK VLSSVPEIVELVQC DAEKFSHDS QDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYV 300
           VR+A+CKLKRRLS EKKKRQLSAKEQLILRLE QYPADVGILAA+FLNYV+LKPGEALYV
Sbjct: 241 VRDAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSF SVPGPSLFLV+SGKGTIITGYSEE+TLQEGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFASVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITATAIELHMYRAGINNRFFRD 450
           FVPAYMEVSIT+ A EL +YRAG+NNRFF D
Sbjct: 421 FVPAYMEVSITSGATELRLYRAGVNNRFFGD 451

BLAST of CsGy5G005000 vs. TAIR 10
Match: AT3G02570.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 450.7 bits (1158), Expect = 1.4e-126
Identity = 233/428 (54.44%), Postives = 315/428 (73.60%), Query Frame = 0

Query: 20  ADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNVDPGECYAEFWIGTHKSGPS 79
           ADRR RL RL+C V++YDWG IG +S V R++  NS    +DP   YAE W+GTH+SGPS
Sbjct: 15  ADRR-RLRRLRCSVKDYDWGKIGSDSLVYRVYAANSD-YEIDPTRPYAELWMGTHESGPS 74

Query: 80  FVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKWGGDLPFLFKVLSIEKALSI 139
           ++          A GS  ++L+  I+ +P + LG++V  KWG DLPFLFKVLS+ + LSI
Sbjct: 75  YL--------EDADGSNGVTLRSWITENPKS-LGNRVLEKWGCDLPFLFKVLSVARPLSI 134

Query: 140 QAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCGFISSKELKAVLSSVPEIVE 199
           QAHPDK LA+ +++A P++YKDDNHKPEMALA T+FEALCGFI  +ELK+V+ ++PEI E
Sbjct: 135 QAHPDKKLAKKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEE 194

Query: 200 LVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIVREAVCKLKRRLSLEKKKRQ 259
           LV   +A +    +E D +EKVK +  +IF+ +MS++    ++ V KLKRRL +E ++RQ
Sbjct: 195 LVGSEEANQVFCITEHD-EEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQ 254

Query: 260 LSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVGPNEPHAYISGECIECMATS 319
           L+ KE+L+L+LE QYP D+G+++A+F NYV+L PGEALY+G NEPHAY+ GEC+E MATS
Sbjct: 255 LTDKERLVLKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATS 314

Query: 320 DNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYTRKYAPPFDEFEVDRCILPQ 379
           DNVVRAGLTSK  D+Q L SML+YK G+PEIL+G  +  Y  +Y PPF+EFEVD C LP 
Sbjct: 315 DNVVRAGLTSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPS 374

Query: 380 AASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLFVPAYMEVSITATAIELHMY 439
            AS  FPSVPGPSL LV+ G+G + T  S +  +  G+VLFVPA  E+ + +++ +L +Y
Sbjct: 375 GASTVFPSVPGPSLLLVLQGEGRMSTEASAD-GISMGDVLFVPADTEIHLRSSS-DLKLY 428

Query: 440 RAGINNRF 448
           RAGIN+RF
Sbjct: 435 RAGINSRF 428

BLAST of CsGy5G005000 vs. TAIR 10
Match: AT1G67070.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 450.3 bits (1157), Expect = 1.8e-126
Identity = 226/428 (52.80%), Postives = 305/428 (71.26%), Query Frame = 0

Query: 24  KRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNVDPGECYAEFWIGTHKSGPSFVVY 83
           + + RL+C V+NY+WG +G  S VARL   N+G+  VD    YAEFW+GTH+SGPS V +
Sbjct: 20  EEIQRLRCFVKNYEWGKLGPESLVARLQEANTGQ-RVDSEIPYAEFWMGTHESGPSHVEF 79

Query: 84  GRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHP 143
           G  +          ++LK  + +D   +LG KV  KWG DLPFLFKVLS+ KALSIQAHP
Sbjct: 80  GSGH---GVSDKCMVTLKSWV-LDNPNLLGSKVVDKWGCDLPFLFKVLSVTKALSIQAHP 139

Query: 144 DKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCGFISSKELKAVLSSVPEIVELVQC 203
           +K LA  L+   P +Y+D+NHKPE+ALA+T F+ALCGF++ KELK V+++VPEI ELV  
Sbjct: 140 NKALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVGS 199

Query: 204 ADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIVREAVCKLKRRLSLEKKKRQLSAK 263
             A++  +  E D  E++K +   IF+Q+MS++    ++ V ++K RL LE K R+LS K
Sbjct: 200 KAADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSEK 259

Query: 264 EQLILRLESQYPADVGILAAYFLNYVELKPGEALYVGPNEPHAYISGECIECMATSDNVV 323
           E+L+L LE QY  D+G+++A+F NYV+L PGEALY+  NEPHAYISG+C+ECMA SDNVV
Sbjct: 260 EKLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNVV 319

Query: 324 RAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYTRKYAPPFDEFEVDRCILPQAASV 383
           RAGLT K RDVQ L SML YK G+PEIL+G  L  Y  +Y PPFDEFEVD C LP+  S 
Sbjct: 320 RAGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKST 379

Query: 384 SFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLFVPAYMEVSITATAIELHMYRAGI 443
            FP+VPGPS++LV+ GKG + TG S +  +  G+VLFVPA +E+ +T  +  + +YRAG+
Sbjct: 380 VFPAVPGPSVYLVIEGKGQLRTG-SSKVLVNRGDVLFVPADIEIHVTGESDVMKLYRAGV 439

Query: 444 NNRFFRDL 452
           ++RFF+ L
Sbjct: 440 SSRFFQTL 441

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M8842.0e-12554.44Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=... [more]
Q9FZH52.6e-12552.80Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=... [more]
Q54PA01.7e-7639.41Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=m... [more]
Q9HFU47.4e-7240.00Mannose-6-phosphate isomerase OS=Cryptococcus neoformans var. neoformans serotyp... [more]
Q9GP383.1e-7038.53Probable mannose-6-phosphate isomerase OS=Echinococcus multilocularis OX=6211 GN... [more]
Match NameE-valueIdentityDescription
XP_004143675.10.0100.00mannose-6-phosphate isomerase 1 [Cucumis sativus] >XP_011654856.1 mannose-6-phos... [more]
XP_038875726.14.09e-30994.24mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875727.1 mannose... [more]
XP_023511752.13.76e-29188.69mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022921753.11.53e-29088.69mannose-6-phosphate isomerase 1-like [Cucurbita moschata][more]
XP_023549674.12.34e-29089.60mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KL160.0100.00Mannose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_5G167200 PE=3 SV... [more]
A0A6J1E4R07.40e-29188.69Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111429906 PE=3... [more]
A0A6J1I4432.12e-29088.47Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111470445 PE=3 S... [more]
A0A6J1FJN31.32e-28989.16Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111444656 PE=3... [more]
A0A6J1K0476.25e-28889.14Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111489838 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT3G02570.11.4e-12654.44Mannose-6-phosphate isomerase, type I [more]
AT1G67070.11.8e-12652.80Mannose-6-phosphate isomerase, type I [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 253..273
NoneNo IPR availableGENE3D1.10.441.10Phosphomannose Isomerase, domain 2coord: 183..349
e-value: 2.1E-132
score: 444.2
NoneNo IPR availablePIRSRPIRSR001480-2PIRSR001480-2coord: 34..441
e-value: 1.3E-123
score: 411.0
NoneNo IPR availablePIRSRPIRSR001480-1PIRSR001480-1coord: 34..441
e-value: 1.3E-123
score: 411.0
NoneNo IPR availablePANTHERPTHR10309:SF10MANNOSE-6-PHOSPHATE ISOMERASEcoord: 16..449
NoneNo IPR availableCDDcd07011cupin_PMI_type_I_Ncoord: 29..343
e-value: 3.57311E-115
score: 336.059
NoneNo IPR availableCDDcd02208cupin_RmlC-likecoord: 393..435
e-value: 8.13008E-5
score: 39.003
IPR016305Mannose-6-phosphate isomerasePRINTSPR00714MAN6PISMRASEcoord: 270..285
score: 38.75
coord: 65..80
score: 53.12
coord: 29..47
score: 46.32
coord: 286..305
score: 48.0
coord: 324..343
score: 52.0
coord: 159..182
score: 68.75
coord: 305..324
score: 70.0
IPR016305Mannose-6-phosphate isomerasePIRSFPIRSF001480PMIcoord: 23..449
e-value: 1.3E-126
score: 421.1
IPR016305Mannose-6-phosphate isomerasePANTHERPTHR10309MANNOSE-6-PHOSPHATE ISOMERASEcoord: 16..449
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 26..441
e-value: 2.1E-132
score: 444.2
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 30..325
e-value: 2.1E-132
score: 444.2
IPR001250Mannose-6-phosphate isomerase, type IPFAMPF01238PMI_typeIcoord: 26..406
e-value: 3.2E-101
score: 339.2
IPR001250Mannose-6-phosphate isomerase, type ITIGRFAMTIGR00218TIGR00218coord: 26..432
e-value: 4.3E-75
score: 251.1
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00965PMI_I_1coord: 159..167
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 25..443

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G005000.2CsGy5G005000.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0000032 cell wall mannoprotein biosynthetic process
biological_process GO:0009298 GDP-mannose biosynthetic process
biological_process GO:0006486 protein glycosylation
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004476 mannose-6-phosphate isomerase activity
molecular_function GO:0008270 zinc ion binding