CsGy4G025630 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G025630
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionsplicing factor U2af small subunit B-like
LocationGy14Chr4: 30886228 .. 30887058 (-)
RNA-Seq ExpressionCsGy4G025630
SyntenyCsGy4G025630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAACACTTGGCCTCAATATTTGGCACCGAGAAGGACCGTGTCAACTGCCCTTTCTATTTCAAGATCGGCGCCTGCCGCCATGGAGATCGCTGCTCTCGCCTCCACAACCGCCCTACTATTTCCCCTACTCTTCTCCTATCCAACATGTACCAGCGCCCCGACATGATCACCCCGGGCGTTGATGCTCAGGGCCAGCCCATAGATCCCCGAAAGATTCAGGAACACTTCGAAGATTTCTACGAAGACATTTACGAGGAACTTGGCAAGTTCGGTGAGATCGAGAGCCTTAATGTCTGTGATAATCTCGCCGACCACATGATTGGAAATGTCTACGTTCAGTTTAGGGAGGAGGACCAGGCTGCCGCCGCACTCCAAGCCTTGCAAGGGCGGTTTTACTCAGGTCGACCCATTATCGCAGATTTTTCTCCCGTAACTGATTTCCGCGAAGCCACGTGCCGCCAGTACGAGGAGAATAATTGCAACCGAGGGGGATATTGCAATTTTATGCACGTAAAGATGATTGGGAAGGATTTGAGAAGGAAGCTGTTCGGTAGGTACCGGGGATATAGGGCGAGCCGGAGCAGGAGTAGAAGTTTGAGTCCGCGGAACCGGAAAGAGCACGATAGGCGTGAAAGGGATTACAGAGACCGTGACTATCGCGGAAACGGACGGAGCAAGGAGCGTCATGATAGGGATGGGGGAAGGAGAAGACAAGGTAGCCCCAGACGAAGCCGAAGCCGAAGCCCTGTCATTGTGAGAGAAGGTAGCGAAGAGCGACGTGCCCGTATTGAACAGTGGAACCGAGAAAGGGAGGAGAAGCAGTGA

mRNA sequence

ATGGCTGAACACTTGGCCTCAATATTTGGCACCGAGAAGGACCGTGTCAACTGCCCTTTCTATTTCAAGATCGGCGCCTGCCGCCATGGAGATCGCTGCTCTCGCCTCCACAACCGCCCTACTATTTCCCCTACTCTTCTCCTATCCAACATGTACCAGCGCCCCGACATGATCACCCCGGGCGTTGATGCTCAGGGCCAGCCCATAGATCCCCGAAAGATTCAGGAACACTTCGAAGATTTCTACGAAGACATTTACGAGGAACTTGGCAAGTTCGGTGAGATCGAGAGCCTTAATGTCTGTGATAATCTCGCCGACCACATGATTGGAAATGTCTACGTTCAGTTTAGGGAGGAGGACCAGGCTGCCGCCGCACTCCAAGCCTTGCAAGGGCGGTTTTACTCAGGTCGACCCATTATCGCAGATTTTTCTCCCGTAACTGATTTCCGCGAAGCCACGTGCCGCCAGTACGAGGAGAATAATTGCAACCGAGGGGGATATTGCAATTTTATGCACGTAAAGATGATTGGGAAGGATTTGAGAAGGAAGCTGTTCGGTAGGTACCGGGGATATAGGGCGAGCCGGAGCAGGAGTAGAAGTTTGAGTCCGCGGAACCGGAAAGAGCACGATAGGCGTGAAAGGGATTACAGAGACCGTGACTATCGCGGAAACGGACGGAGCAAGGAGCGTCATGATAGGGATGGGGGAAGGAGAAGACAAGGTAGCCCCAGACGAAGCCGAAGCCGAAGCCCTGTCATTGTGAGAGAAGGTAGCGAAGAGCGACGTGCCCGTATTGAACAGTGGAACCGAGAAAGGGAGGAGAAGCAGTGA

Coding sequence (CDS)

ATGGCTGAACACTTGGCCTCAATATTTGGCACCGAGAAGGACCGTGTCAACTGCCCTTTCTATTTCAAGATCGGCGCCTGCCGCCATGGAGATCGCTGCTCTCGCCTCCACAACCGCCCTACTATTTCCCCTACTCTTCTCCTATCCAACATGTACCAGCGCCCCGACATGATCACCCCGGGCGTTGATGCTCAGGGCCAGCCCATAGATCCCCGAAAGATTCAGGAACACTTCGAAGATTTCTACGAAGACATTTACGAGGAACTTGGCAAGTTCGGTGAGATCGAGAGCCTTAATGTCTGTGATAATCTCGCCGACCACATGATTGGAAATGTCTACGTTCAGTTTAGGGAGGAGGACCAGGCTGCCGCCGCACTCCAAGCCTTGCAAGGGCGGTTTTACTCAGGTCGACCCATTATCGCAGATTTTTCTCCCGTAACTGATTTCCGCGAAGCCACGTGCCGCCAGTACGAGGAGAATAATTGCAACCGAGGGGGATATTGCAATTTTATGCACGTAAAGATGATTGGGAAGGATTTGAGAAGGAAGCTGTTCGGTAGGTACCGGGGATATAGGGCGAGCCGGAGCAGGAGTAGAAGTTTGAGTCCGCGGAACCGGAAAGAGCACGATAGGCGTGAAAGGGATTACAGAGACCGTGACTATCGCGGAAACGGACGGAGCAAGGAGCGTCATGATAGGGATGGGGGAAGGAGAAGACAAGGTAGCCCCAGACGAAGCCGAAGCCGAAGCCCTGTCATTGTGAGAGAAGGTAGCGAAGAGCGACGTGCCCGTATTGAACAGTGGAACCGAGAAAGGGAGGAGAAGCAGTGA

Protein sequence

MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITPGVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDLRRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ*
Homology
BLAST of CsGy4G025630 vs. ExPASy Swiss-Prot
Match: Q9FMY5 (Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 6.5e-127
Identity = 238/283 (84.10%), Postives = 252/283 (89.05%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVD QGQP+DP KIQ+HFEDFYEDI+EEL KFGE+ESLNVCDNLADHMIGNVYV F+EED
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
            AAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEEN+CNRGGYCNFMHVK I ++L
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RRKLFGRYRG--YRASRSRSRSLSPRNRKEHDR-RER-DYRDRDYRGNGR----SKERHD 240
           RRKLFGRYR    R SRSRSRS+SPR ++EH R RER D RDRD  GNG+      ERHD
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

Query: 241 RD-GGRRRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREE 275
           RD GGRRR GSP+  RSRSP  VREGSEERRARIEQWNRER+E
Sbjct: 241 RDGGGRRRHGSPK--RSRSPRNVREGSEERRARIEQWNRERDE 281

BLAST of CsGy4G025630 vs. ExPASy Swiss-Prot
Match: Q9S709 (Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A PE=1 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.0e-124
Identity = 238/294 (80.95%), Postives = 259/294 (88.10%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQP+DPRKIQEHFEDF+ED++EELGKFGEIESLN+CDNLADHMIGNVYVQF+EED
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVK++ ++L
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSREL 180

Query: 181 RRKLFGRY-RGYR---ASRSRSRSLSPRNRKEHDR-----RERDYRDRD---YR-GNGR- 240
           RRKLFGRY R YR    SRSRSRS+SPRN++++DR     RE  +RDRD   YR G+G+ 
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGKR 240

Query: 241 ---SKERHDRDGGR-RRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 277
                ER +RDG R RRQ SP+  R  SP   REGSEERRARIEQWNREREEK+
Sbjct: 241 SSERSERQERDGSRGRRQASPK--RGGSPGGGREGSEERRARIEQWNREREEKE 292

BLAST of CsGy4G025630 vs. ExPASy Swiss-Prot
Match: Q6AUG0 (Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35B PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 3.4e-112
Identity = 223/308 (72.40%), Postives = 244/308 (79.22%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPT+SPT++L+NMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTVSPTIVLANMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDP K+QEHFEDFYEDIYEEL KFGE+E+LNVCDNLADHMIGNVYVQFREE+
Sbjct: 61  GVDAQGQPIDPEKMQEHFEDFYEDIYEELSKFGEVETLNVCDNLADHMIGNVYVQFREEE 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QA AA  ALQGRFYSGRPII ++SPVTDFREATCRQ+EEN+CNRGGYCNFMHVK IG++L
Sbjct: 121 QAVAAHNALQGRFYSGRPIIVEYSPVTDFREATCRQFEENSCNRGGYCNFMHVKQIGREL 180

Query: 181 RRKLFGRYRGYRASRSRSRSLSPRNR-----KEHDRRERD-------------------- 240
           RRKL+G     R S  RSRS SPR+R     ++  RRERD                    
Sbjct: 181 RRKLYGGRS--RRSHGRSRSPSPRHRRGNRDRDDFRRERDGYRGGGDGYRGGGGGGGGDG 240

Query: 241 YRDRD-YRGNG-----RSKERHDR--DGGRRRQGSPRRSRSRSPVIVREGSEERRARIEQ 276
           YR  D YRG G         R+DR  DGGRRR GSP R R+RSP  VRE SEERRA+IEQ
Sbjct: 241 YRGGDSYRGGGGGGRRGGGSRYDRYDDGGRRRHGSPPR-RARSP--VRESSEERRAKIEQ 300

BLAST of CsGy4G025630 vs. ExPASy Swiss-Prot
Match: Q9ZQW8 (Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35A PE=2 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 8.0e-109
Identity = 214/295 (72.54%), Postives = 236/295 (80.00%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHN+P++SPTLLLSNMY RPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNKPSVSPTLLLSNMYLRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           G+DAQG PIDP KIQ  FEDFYEDI+EEL K+GEIESL+VCDN ADHMIGNVYVQFREED
Sbjct: 61  GIDAQGNPIDPEKIQADFEDFYEDIFEELSKYGEIESLHVCDNFADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAA ALQAL GR+YSGRPII +FSPV+DFREATCRQYEEN+CNRGGYCNFMHVK IG+DL
Sbjct: 121 QAARALQALTGRYYSGRPIIVEFSPVSDFREATCRQYEENSCNRGGYCNFMHVKEIGRDL 180

Query: 181 RRKLFGRYRGYRASRSRSRSLSP---RNRKEHDR----RERDYRD------RDY--RGNG 240
           R++LFG     R S S  RS SP     R+++DR    R RD+ D       DY   G+ 
Sbjct: 181 RKRLFGHLHRSRRSHSHGRSRSPSPYHYRRDYDRRSSSRSRDHDDYYRGGSHDYYRGGSR 240

Query: 241 RSKERH----DRDGGRRRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 277
           RS ERH    D DG RRR     RSR+RSP  VR+GSEERRA+IEQWNRERE  Q
Sbjct: 241 RSSERHRSSYDSDGSRRR----HRSRTRSP--VRDGSEERRAQIEQWNREREAAQ 289

BLAST of CsGy4G025630 vs. ExPASy Swiss-Prot
Match: A1A4K8 (Splicing factor U2AF 35 kDa subunit OS=Bos taurus OX=9913 GN=U2AF1 PE=2 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 3.7e-66
Identity = 132/250 (52.80%), Postives = 171/250 (68.40%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAE+LASIFGTEKD+VNC FYFKIGACRHGDRCSRLHN+PT S T+ L N+Y+ P   + 
Sbjct: 1   MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQ 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEEL-GKFGEIESLNVCDNLADHMIGNVYVQFREE 120
             D     +   ++QEH+++F+E+++ E+  K+GE+E +NVCDNL DH++GNVYV+FR E
Sbjct: 61  SADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRRE 120

Query: 121 DQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKD 180
           + A  A+  L  R+++G+PI A+ SPVTDFREA CRQYE   C RGG+CNFMH+K I ++
Sbjct: 121 EDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRE 180

Query: 181 LRRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRR 240
           LRR+L+GR R    SRSRSR          +RR R  RDR   G G         GG  R
Sbjct: 181 LRRELYGRRRKKHRSRSRSR----------ERRSRS-RDRGRGGGG--------GGGGGR 231

Query: 241 QGSPRRSRSR 250
           +   RRSR R
Sbjct: 241 ERDRRRSRDR 231

BLAST of CsGy4G025630 vs. NCBI nr
Match: XP_004145565.1 (splicing factor U2af small subunit B [Cucumis sativus] >KGN55547.1 hypothetical protein Csa_012279 [Cucumis sativus])

HSP 1 Score: 546 bits (1406), Expect = 1.10e-195
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQ 240
           RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQ
Sbjct: 181 RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQ 240

Query: 241 GSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 276
           GSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ
Sbjct: 241 GSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 276

BLAST of CsGy4G025630 vs. NCBI nr
Match: XP_008453027.1 (PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >KAA0064675.1 splicing factor U2af small subunit B-like [Cucumis melo var. makuwa] >TYK19916.1 splicing factor U2af small subunit B-like [Cucumis melo var. makuwa])

HSP 1 Score: 535 bits (1378), Expect = 2.03e-191
Identity = 270/276 (97.83%), Postives = 273/276 (98.91%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQ 240
           RRKLFGRYRGYR SRSRSRSLSPRNRKE+DRRERDYR+R+YRGNGRSKERHDRDGGRRR 
Sbjct: 181 RRKLFGRYRGYRPSRSRSRSLSPRNRKEYDRRERDYREREYRGNGRSKERHDRDGGRRRH 240

Query: 241 GSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 276
           GSPRRSRSRSPVI REGSEERRARIEQWNREREEKQ
Sbjct: 241 GSPRRSRSRSPVIAREGSEERRARIEQWNREREEKQ 276

BLAST of CsGy4G025630 vs. NCBI nr
Match: XP_038900064.1 (splicing factor U2af small subunit B-like [Benincasa hispida])

HSP 1 Score: 529 bits (1363), Expect = 3.66e-189
Identity = 270/276 (97.83%), Postives = 270/276 (97.83%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQ 240
           RRKLFGRYRGYR SRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKER DRDGGRRR 
Sbjct: 181 RRKLFGRYRGYRRSRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERQDRDGGRRRH 240

Query: 241 GSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 276
           GSPRRSRS  PV VREGSEERRARIEQWNREREEKQ
Sbjct: 241 GSPRRSRS--PVTVREGSEERRARIEQWNREREEKQ 274

BLAST of CsGy4G025630 vs. NCBI nr
Match: XP_022142994.1 (splicing factor U2af small subunit B-like [Momordica charantia])

HSP 1 Score: 510 bits (1314), Expect = 1.12e-181
Identity = 263/278 (94.60%), Postives = 268/278 (96.40%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDR---DGGR 240
           RRKLFGRYRGYR SRSRSRSLSPRNRK++DRRERD+RDRD+RGNGRSKER+DR   DGGR
Sbjct: 181 RRKLFGRYRGYRRSRSRSRSLSPRNRKDYDRRERDFRDRDFRGNGRSKERYDRYDRDGGR 240

Query: 241 RRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREEK 275
           RR GSPRRSRS     VREGSEERRARIEQWNREREEK
Sbjct: 241 RRHGSPRRSRS----PVREGSEERRARIEQWNREREEK 274

BLAST of CsGy4G025630 vs. NCBI nr
Match: XP_022941119.1 (splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022941120.1 splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022941121.1 splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022981902.1 splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022981903.1 splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022981905.1 splicing factor U2af small subunit B-like [Cucurbita maxima] >KAG7037979.1 Splicing factor U2af small subunit A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506 bits (1304), Expect = 3.87e-180
Identity = 263/280 (93.93%), Postives = 269/280 (96.07%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLN+CDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFG-RYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKER---HDRDGG 240
           RRKLFG R+RGYR SRSRSRSLSPRNRK++DRRERDYRDRD+RGNGRSKER   +DRDGG
Sbjct: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240

Query: 241 RRRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 276
           RRR GSPRRSRS     VREGSEERRARIEQWNREREEKQ
Sbjct: 241 RRRHGSPRRSRS----PVREGSEERRARIEQWNREREEKQ 276

BLAST of CsGy4G025630 vs. ExPASy TrEMBL
Match: A0A0A0L4P8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G664540 PE=4 SV=1)

HSP 1 Score: 546 bits (1406), Expect = 5.31e-196
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQ 240
           RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQ
Sbjct: 181 RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQ 240

Query: 241 GSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 276
           GSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ
Sbjct: 241 GSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 276

BLAST of CsGy4G025630 vs. ExPASy TrEMBL
Match: A0A5A7VFL1 (Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00280 PE=4 SV=1)

HSP 1 Score: 535 bits (1378), Expect = 9.85e-192
Identity = 270/276 (97.83%), Postives = 273/276 (98.91%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQ 240
           RRKLFGRYRGYR SRSRSRSLSPRNRKE+DRRERDYR+R+YRGNGRSKERHDRDGGRRR 
Sbjct: 181 RRKLFGRYRGYRPSRSRSRSLSPRNRKEYDRRERDYREREYRGNGRSKERHDRDGGRRRH 240

Query: 241 GSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 276
           GSPRRSRSRSPVI REGSEERRARIEQWNREREEKQ
Sbjct: 241 GSPRRSRSRSPVIAREGSEERRARIEQWNREREEKQ 276

BLAST of CsGy4G025630 vs. ExPASy TrEMBL
Match: A0A1S3BUN6 (splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC103493856 PE=4 SV=1)

HSP 1 Score: 535 bits (1378), Expect = 9.85e-192
Identity = 270/276 (97.83%), Postives = 273/276 (98.91%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDRDGGRRRQ 240
           RRKLFGRYRGYR SRSRSRSLSPRNRKE+DRRERDYR+R+YRGNGRSKERHDRDGGRRR 
Sbjct: 181 RRKLFGRYRGYRPSRSRSRSLSPRNRKEYDRRERDYREREYRGNGRSKERHDRDGGRRRH 240

Query: 241 GSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 276
           GSPRRSRSRSPVI REGSEERRARIEQWNREREEKQ
Sbjct: 241 GSPRRSRSRSPVIAREGSEERRARIEQWNREREEKQ 276

BLAST of CsGy4G025630 vs. ExPASy TrEMBL
Match: A0A6J1CN17 (splicing factor U2af small subunit B-like OS=Momordica charantia OX=3673 GN=LOC111012985 PE=4 SV=1)

HSP 1 Score: 510 bits (1314), Expect = 5.41e-182
Identity = 263/278 (94.60%), Postives = 268/278 (96.40%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFGRYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKERHDR---DGGR 240
           RRKLFGRYRGYR SRSRSRSLSPRNRK++DRRERD+RDRD+RGNGRSKER+DR   DGGR
Sbjct: 181 RRKLFGRYRGYRRSRSRSRSLSPRNRKDYDRRERDFRDRDFRGNGRSKERYDRYDRDGGR 240

Query: 241 RRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREEK 275
           RR GSPRRSRS     VREGSEERRARIEQWNREREEK
Sbjct: 241 RRHGSPRRSRS----PVREGSEERRARIEQWNREREEK 274

BLAST of CsGy4G025630 vs. ExPASy TrEMBL
Match: A0A6J1IV82 (splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC111480904 PE=4 SV=1)

HSP 1 Score: 506 bits (1304), Expect = 1.87e-180
Identity = 263/280 (93.93%), Postives = 269/280 (96.07%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLN+CDNLADHMIGNVYVQFREED
Sbjct: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNICDNLADHMIGNVYVQFREED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180

Query: 181 RRKLFG-RYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDYRGNGRSKER---HDRDGG 240
           RRKLFG R+RGYR SRSRSRSLSPRNRK++DRRERDYRDRD+RGNGRSKER   +DRDGG
Sbjct: 181 RRKLFGSRFRGYRRSRSRSRSLSPRNRKDYDRRERDYRDRDHRGNGRSKERPDRYDRDGG 240

Query: 241 RRRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 276
           RRR GSPRRSRS     VREGSEERRARIEQWNREREEKQ
Sbjct: 241 RRRHGSPRRSRS----PVREGSEERRARIEQWNREREEKQ 276

BLAST of CsGy4G025630 vs. TAIR 10
Match: AT5G42820.2 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 454.9 bits (1169), Expect = 4.6e-128
Identity = 238/283 (84.10%), Postives = 252/283 (89.05%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVD QGQP+DP KIQ+HFEDFYEDI+EEL KFGE+ESLNVCDNLADHMIGNVYV F+EED
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
            AAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEEN+CNRGGYCNFMHVK I ++L
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RRKLFGRYRG--YRASRSRSRSLSPRNRKEHDR-RER-DYRDRDYRGNGR----SKERHD 240
           RRKLFGRYR    R SRSRSRS+SPR ++EH R RER D RDRD  GNG+      ERHD
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

Query: 241 RD-GGRRRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREE 275
           RD GGRRR GSP+  RSRSP  VREGSEERRARIEQWNRER+E
Sbjct: 241 RDGGGRRRHGSPK--RSRSPRNVREGSEERRARIEQWNRERDE 281

BLAST of CsGy4G025630 vs. TAIR 10
Match: AT5G42820.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 454.9 bits (1169), Expect = 4.6e-128
Identity = 238/283 (84.10%), Postives = 252/283 (89.05%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVD QGQP+DP KIQ+HFEDFYEDI+EEL KFGE+ESLNVCDNLADHMIGNVYV F+EED
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
            AAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEEN+CNRGGYCNFMHVK I ++L
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RRKLFGRYRG--YRASRSRSRSLSPRNRKEHDR-RER-DYRDRDYRGNGR----SKERHD 240
           RRKLFGRYR    R SRSRSRS+SPR ++EH R RER D RDRD  GNG+      ERHD
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

Query: 241 RD-GGRRRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREE 275
           RD GGRRR GSP+  RSRSP  VREGSEERRARIEQWNRER+E
Sbjct: 241 RDGGGRRRHGSPK--RSRSPRNVREGSEERRARIEQWNRERDE 281

BLAST of CsGy4G025630 vs. TAIR 10
Match: AT1G27650.1 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 447.6 bits (1150), Expect = 7.3e-126
Identity = 238/294 (80.95%), Postives = 259/294 (88.10%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREED 120
           GVDAQGQP+DPRKIQEHFEDF+ED++EELGKFGEIESLN+CDNLADHMIGNVYVQF+EED
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKMIGKDL 180
           QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVK++ ++L
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSREL 180

Query: 181 RRKLFGRY-RGYR---ASRSRSRSLSPRNRKEHDR-----RERDYRDRD---YR-GNGR- 240
           RRKLFGRY R YR    SRSRSRS+SPRN++++DR     RE  +RDRD   YR G+G+ 
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGKR 240

Query: 241 ---SKERHDRDGGR-RRQGSPRRSRSRSPVIVREGSEERRARIEQWNREREEKQ 277
                ER +RDG R RRQ SP+  R  SP   REGSEERRARIEQWNREREEK+
Sbjct: 241 SSERSERQERDGSRGRRQASPK--RGGSPGGGREGSEERRARIEQWNREREEKE 292

BLAST of CsGy4G025630 vs. TAIR 10
Match: AT1G27650.2 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 339.0 bits (868), Expect = 3.7e-93
Identity = 188/244 (77.05%), Postives = 209/244 (85.66%), Query Frame = 0

Query: 51  MYQRPDMITPGVDAQGQPIDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIG 110
           MYQRPDMITPGVDAQGQP+DPRKIQEHFEDF+ED++EELGKFGEIESLN+CDNLADHMIG
Sbjct: 1   MYQRPDMITPGVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIG 60

Query: 111 NVYVQFREEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNF 170
           NVYVQF+EEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNF
Sbjct: 61  NVYVQFKEEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNF 120

Query: 171 MHVKMIGKDLRRKLFGRY-RGYR---ASRSRSRSLSPRNRKEHDR-----RERDYRDRD- 230
           MHVK++ ++LRRKLFGRY R YR    SRSRSRS+SPRN++++DR     RE  +RDRD 
Sbjct: 121 MHVKLVSRELRRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDR 180

Query: 231 --YR-GNGR----SKERHDRDGGR-RRQGSPRRSRSRSPVIVREGSEERRARIEQWNRER 277
             YR G+G+      ER +RDG R RRQ SP+  R  SP   REGSEERRARIEQWNRER
Sbjct: 181 EFYRHGSGKRSSERSERQERDGSRGRRQASPK--RGGSPGGGREGSEERRARIEQWNRER 240

BLAST of CsGy4G025630 vs. TAIR 10
Match: AT1G10320.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 133.3 bits (334), Expect = 3.1e-31
Identity = 82/237 (34.60%), Postives = 129/237 (54.43%), Query Frame = 0

Query: 9   FGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITPGVDAQGQP 68
           FGTE+D+ +CPF+ K GACR G RCSR+H  P  S TLL+ NMY  P  IT   D +G  
Sbjct: 237 FGTEQDKAHCPFHLKTGACRFGQRCSRVHFYPNKSCTLLMKNMYNGPG-ITWEQD-EGLE 296

Query: 69  IDPRKIQEHFEDFYEDIYEELGKFGEIESLNVCDNLADHMIGNVYVQFREEDQAAAALQA 128
               + +  +E+FYED++ E  K+GE+ +  VC N + H+ GNVYV +R  + A  A Q+
Sbjct: 297 YTDEEAELCYEEFYEDVHTEFLKYGELVNFKVCRNGSFHLKGNVYVHYRSLESAILAYQS 356

Query: 129 LQGRFYSGRPIIADFSPVTDFREATCRQYEEN---NCNRGGYCNFMH-VKMIGKDLR--- 188
           + GR+++G+ +  +F  ++ ++ A C +Y ++    C+RG  CNF+H  +  G D     
Sbjct: 357 INGRYFAGKQVNCEFVNISRWKVAICGEYMKSRLKTCSRGSACNFIHCFRNPGGDYEWAD 416

Query: 189 -------------RKLFG----RYRGYRASRSRSRSLSPRNRKEHDRRERDYRDRDY 222
                          LFG    ++  + +S S + S+S  +   H +  R  R RD+
Sbjct: 417 HDRPPPRFWIHKMTSLFGYSDEKHMEHESSGSLNDSISDLSTDSHRQPSRRSRSRDH 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMY56.5e-12784.10Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B ... [more]
Q9S7091.0e-12480.95Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A ... [more]
Q6AUG03.4e-11272.40Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q9ZQW88.0e-10972.54Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
A1A4K83.7e-6652.80Splicing factor U2AF 35 kDa subunit OS=Bos taurus OX=9913 GN=U2AF1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004145565.11.10e-195100.00splicing factor U2af small subunit B [Cucumis sativus] >KGN55547.1 hypothetical ... [more]
XP_008453027.12.03e-19197.83PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >KAA0064675.... [more]
XP_038900064.13.66e-18997.83splicing factor U2af small subunit B-like [Benincasa hispida][more]
XP_022142994.11.12e-18194.60splicing factor U2af small subunit B-like [Momordica charantia][more]
XP_022941119.13.87e-18093.93splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022941120.1 s... [more]
Match NameE-valueIdentityDescription
A0A0A0L4P85.31e-196100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G664540 PE=4 SV=1[more]
A0A5A7VFL19.85e-19297.83Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BUN69.85e-19297.83splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC10349385... [more]
A0A6J1CN175.41e-18294.60splicing factor U2af small subunit B-like OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1IV821.87e-18093.93splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT5G42820.24.6e-12884.10Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
AT5G42820.14.6e-12884.10Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
AT1G27650.17.3e-12680.95U2 snRNP auxiliary factor small subunit, putative [more]
AT1G27650.23.7e-9377.05U2 snRNP auxiliary factor small subunit, putative [more]
AT1G10320.13.1e-3134.60Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 258..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 201..241
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 195..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 249..276
NoneNo IPR availablePANTHERPTHR12620:SF35SPLICING FACTOR U2AF SMALL SUBUNIT Acoord: 1..276
NoneNo IPR availableCDDcd12539RRM_U2AF35Bcoord: 44..145
e-value: 2.23244E-60
score: 184.875
IPR009145U2 auxiliary factor small subunitPRINTSPR01848U2AUXFACTORcoord: 165..177
score: 63.29
coord: 75..90
score: 54.26
coord: 103..125
score: 65.61
coord: 18..37
score: 85.68
coord: 37..57
score: 62.34
coord: 130..154
score: 63.64
IPR009145U2 auxiliary factor small subunitPANTHERPTHR12620U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNITcoord: 1..276
IPR003954RNA recognition motif domain, eukaryoteSMARTSM00361rrm2_1coord: 73..142
e-value: 1.5E-4
score: 31.1
IPR000571Zinc finger, CCCH-typeSMARTSM00356c3hfinal6coord: 148..174
e-value: 0.0087
score: 25.3
coord: 13..39
e-value: 0.0068
score: 25.6
IPR000571Zinc finger, CCCH-typePFAMPF00642zf-CCCHcoord: 150..174
e-value: 1.4E-4
score: 21.7
coord: 14..38
e-value: 2.8E-7
score: 30.3
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 12..40
score: 13.065497
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 148..175
score: 11.413015
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 83..139
e-value: 1.2E-7
score: 31.5
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 44..146
score: 11.05946
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 43..145
e-value: 8.2E-31
score: 108.4
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 77..149

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G025630.1CsGy4G025630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005681 spliceosomal complex
cellular_component GO:0089701 U2AF complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030628 pre-mRNA 3'-splice site binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003723 RNA binding