CsGy4G024243 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy4G024243
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Threonyl-tRNA synthetase
Location	Gy14Chr4: 30032542 .. 30033293 (-)
RNA-Seq Expression	CsGy4G024243
Synteny	CsGy4G024243

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACCATAAATTACACTCTCTTACCTCACCATTCTTTCGAACTACTTTCCCAGGTTTCCAGCCTCTTCAGGTGATATTGTTTCTTCCGTTTCCTTTGTTATTATTTTGAAATTTCTTTCATATTTTGGTGGGTTTGTATATTATCAGCGTTTTCTTTTGTTCTTTCTCACATTTTGGCTGTTTATTTGGGTTCTAATTTGATTTTTTTTATGCTTAATGAAGGATTTTCTTAATGGAGTTCCCCTGGGTCAAGTTTAGGTTTGAACTAGGCAGGAGTTTCATTGCTTAAACAGGGTGTTTCTAACCTAGATGGGGTTTTCTGAATTCCATTTTGCATCTTGGAGATAAGATTGAATGCGGACTTCAGGTTTCAACTGCTTGATATATGCTTTTCAAATTGCAGTGTGCTCATGTAATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTGCTATGATTTTGATATAGAGCCCTTGGGAGATAAAGATTTGATGAAAATCAAGAAGGAGATGGTTGGTATTATACCAAATCAATGAGCATTGAAGTCACTGGTTTTCTTTTTCTTTATTATTTTACATCACATGATTTGGAATATATGCATTAGTCTATGCTTTTAGTATGTGGGGTTTATTTTTCATATCTTCTCTAGGAACGCATTATCAGCACAAACTTACCACTTGTAAGAGAAGAAGTTTCAAGGGATAAAGCTTAA

mRNA sequence

ATGTACCATAAATTACACTCTCTTACCTCACCATTCTTTCGAACTACTTTCCCAGGTTTCCAGCCTCTTCAGTGTGCTCATGTAATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTGCTATGATTTTGATATAGAGCCCTTGGGAGATAAAGATTTGATGAAAATCAAGAAGGAGATGGAACGCATTATCAGCACAAACTTACCACTTGTAAGAGAAGAAGTTTCAAGGGATAAAGCTTAA

Coding sequence (CDS)

ATGTACCATAAATTACACTCTCTTACCTCACCATTCTTTCGAACTACTTTCCCAGGTTTCCAGCCTCTTCAGTGTGCTCATGTAATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTGCTATGATTTTGATATAGAGCCCTTGGGAGATAAAGATTTGATGAAAATCAAGAAGGAGATGGAACGCATTATCAGCACAAACTTACCACTTGTAAGAGAAGAAGTTTCAAGGGATAAAGCTTAA

Protein sequence

MYHKLHSLTSPFFRTTFPGFQPLQCAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEVSRDKA*

Homology

[Close]

BLAST of CsGy4G024243 vs. ExPASy Swiss-Prot
Match: F4IFC5 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2761 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 2.3e-28
Identity = 58/69 (84.06%), Postives = 65/69 (94.20%), Query Frame = 0


Query: 25  CAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLV 84
           CAHVMAMAVQKLFPDAKVTIGPWI+NGF YDFD+EPL DKDL +IKKEM+RIIS NLPL+
Sbjct: 86  CAHVMAMAVQKLFPDAKVTIGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLL 145

Query: 85  REEVSRDKA 94
           REEVSR++A
Sbjct: 146 REEVSREEA 154

[Close]

BLAST of CsGy4G024243 vs. ExPASy Swiss-Prot
Match: Q8DGW0 (Threonine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=thrS PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.2e-19
Identity = 45/69 (65.22%), Postives = 57/69 (82.61%), Query Frame = 0


Query: 26 AHVMAMAVQKLFPDAKVTIGPWIENGFCYDFD-IEPLGDKDLMKIKKEMERIISTNLPLV 85
          +H++AMAVQKLFP A+VTIGPWIENGF YDFD  EP  +KDL  I+KEM +II   LP++
Sbjct: 28 SHILAMAVQKLFPKAQVTIGPWIENGFYYDFDHPEPFSEKDLRLIEKEMVKIIKRKLPVI 87

Query: 86 REEVSRDKA 94
          REEV+R++A
Sbjct: 88 REEVTREEA 96

[Close]

BLAST of CsGy4G024243 vs. ExPASy Swiss-Prot
Match: Q55806 (Threonine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=thrS PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.5e-19
Identity = 45/69 (65.22%), Postives = 56/69 (81.16%), Query Frame = 0


Query: 26 AHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDI-EPLGDKDLMKIKKEMERIISTNLPLV 85
          +HVMAMAVQKLFP A+VTIGPW E GF YDFD+ EP  + DL  IKKEM +II+  LP++
Sbjct: 30 SHVMAMAVQKLFPKAQVTIGPWTETGFYYDFDVAEPFTEADLKAIKKEMVKIINKKLPVI 89

Query: 86 REEVSRDKA 94
          REE+SR++A
Sbjct: 90 REEISREEA 98

[Close]

BLAST of CsGy4G024243 vs. ExPASy Swiss-Prot
Match: Q31JY3 (Threonine--tRNA ligase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=thrS PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 3.4e-19
Identity = 47/69 (68.12%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 26 AHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDI-EPLGDKDLMKIKKEMERIISTNLPLV 85
          +HVMAMAVQKLFP A+VTIGPW E GF YDFD  EP  + DL  IKKEM +II   LP+V
Sbjct: 30 SHVMAMAVQKLFPKAQVTIGPWTETGFYYDFDTPEPFTEADLKAIKKEMVKIIQQKLPVV 89

Query: 86 REEVSRDKA 94
          REEVSR++A
Sbjct: 90 REEVSREEA 98

[Close]

BLAST of CsGy4G024243 vs. ExPASy Swiss-Prot
Match: Q5N1X6 (Threonine--tRNA ligase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=thrS PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 3.4e-19
Identity = 47/69 (68.12%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 26  AHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDI-EPLGDKDLMKIKKEMERIISTNLPLV 85
           +HVMAMAVQKLFP A+VTIGPW E GF YDFD  EP  + DL  IKKEM +II   LP+V
Sbjct: 33  SHVMAMAVQKLFPKAQVTIGPWTETGFYYDFDTPEPFTEADLKAIKKEMVKIIQQKLPVV 92

Query: 86  REEVSRDKA 94
           REEVSR++A
Sbjct: 93  REEVSREEA 101

[Close]

BLAST of CsGy4G024243 vs. NCBI nr
Match: KAE8649928.1 (hypothetical protein Csa_012436 [Cucumis sativus])

HSP 1 Score: 136 bits (343), Expect = 5.95e-40
Identity = 65/65 (100.00%), Postives = 65/65 (100.00%), Query Frame = 0


Query: 29 MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 88
          MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV
Sbjct: 1  MAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLVREEV 60

Query: 89 SRDKA 93
          SRDKA
Sbjct: 61 SRDKA 65

[Close]

BLAST of CsGy4G024243 vs. NCBI nr
Match: XP_022847786.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2-like isoform X1 [Olea europaea var. sylvestris])

HSP 1 Score: 129 bits (324), Expect = 6.94e-35
Identity = 60/71 (84.51%), Postives = 66/71 (92.96%), Query Frame = 0


Query: 23  LQCAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLP 82
           +QCAHVMAMAVQKL+PDAKVTIGPWIENGF YDFD+EPL DKDL +IKKEM+RII  NLP
Sbjct: 133 VQCAHVMAMAVQKLYPDAKVTIGPWIENGFYYDFDMEPLTDKDLKRIKKEMDRIIGRNLP 192

Query: 83  LVREEVSRDKA 93
           LVREEVSRD+A
Sbjct: 193 LVREEVSRDEA 203

[Close]

BLAST of CsGy4G024243 vs. NCBI nr
Match: KAG6589116.1 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022820.1 Threonine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 135 bits (339), Expect = 1.75e-34
Identity = 64/69 (92.75%), Postives = 66/69 (95.65%), Query Frame = 0


Query: 25  CAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLV 84
           CAHVMAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLV
Sbjct: 102 CAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLV 161

Query: 85  REEVSRDKA 93
           REEVSRD+A
Sbjct: 162 REEVSRDEA 170

[Close]

BLAST of CsGy4G024243 vs. NCBI nr
Match: KAG7011877.1 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 135 bits (339), Expect = 1.79e-34
Identity = 64/69 (92.75%), Postives = 66/69 (95.65%), Query Frame = 0


Query: 25  CAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLV 84
           CAHVMAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLV
Sbjct: 108 CAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLV 167

Query: 85  REEVSRDKA 93
           REEVSRD+A
Sbjct: 168 REEVSRDEA 176

[Close]

BLAST of CsGy4G024243 vs. NCBI nr
Match: XP_004144536.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucumis sativus] >KGN43435.1 hypothetical protein Csa_020587 [Cucumis sativus])

HSP 1 Score: 135 bits (339), Expect = 1.79e-34
Identity = 64/69 (92.75%), Postives = 66/69 (95.65%), Query Frame = 0


Query: 25  CAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLV 84
           CAHVMAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLV
Sbjct: 108 CAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLV 167

Query: 85  REEVSRDKA 93
           REEVSRD+A
Sbjct: 168 REEVSRDEA 176

[Close]

BLAST of CsGy4G024243 vs. ExPASy TrEMBL
Match: A0A0A0L0Y5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G650170 PE=4 SV=1)

HSP 1 Score: 196 bits (499), Expect = 2.76e-63
Identity = 93/93 (100.00%), Postives = 93/93 (100.00%), Query Frame = 0


Query: 1   MYHKLHSLTSPFFRTTFPGFQPLQCAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEP 60
           MYHKLHSLTSPFFRTTFPGFQPLQCAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEP
Sbjct: 32  MYHKLHSLTSPFFRTTFPGFQPLQCAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEP 91

Query: 61  LGDKDLMKIKKEMERIISTNLPLVREEVSRDKA 93
           LGDKDLMKIKKEMERIISTNLPLVREEVSRDKA
Sbjct: 92  LGDKDLMKIKKEMERIISTNLPLVREEVSRDKA 124

[Close]

BLAST of CsGy4G024243 vs. ExPASy TrEMBL
Match: A0A6J1JP39 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111486433 PE=3 SV=1)

HSP 1 Score: 135 bits (339), Expect = 8.69e-35
Identity = 64/69 (92.75%), Postives = 66/69 (95.65%), Query Frame = 0


Query: 25  CAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLV 84
           CAHVMAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLV
Sbjct: 108 CAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLV 167

Query: 85  REEVSRDKA 93
           REEVSRD+A
Sbjct: 168 REEVSRDEA 176

[Close]

BLAST of CsGy4G024243 vs. ExPASy TrEMBL
Match: A0A0A0K179 (Threonyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_7G033430 PE=3 SV=1)

HSP 1 Score: 135 bits (339), Expect = 8.69e-35
Identity = 64/69 (92.75%), Postives = 66/69 (95.65%), Query Frame = 0


Query: 25  CAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLV 84
           CAHVMAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLV
Sbjct: 108 CAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLV 167

Query: 85  REEVSRDKA 93
           REEVSRD+A
Sbjct: 168 REEVSRDEA 176

[Close]

BLAST of CsGy4G024243 vs. ExPASy TrEMBL
Match: A0A6J1GM45 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1)

HSP 1 Score: 135 bits (339), Expect = 8.69e-35
Identity = 64/69 (92.75%), Postives = 66/69 (95.65%), Query Frame = 0


Query: 25  CAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLV 84
           CAHVMAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLV
Sbjct: 108 CAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLV 167

Query: 85  REEVSRDKA 93
           REEVSRD+A
Sbjct: 168 REEVSRDEA 176

[Close]

BLAST of CsGy4G024243 vs. ExPASy TrEMBL
Match: A0A6J1EKH5 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435298 PE=3 SV=1)

HSP 1 Score: 135 bits (339), Expect = 8.69e-35
Identity = 64/69 (92.75%), Postives = 66/69 (95.65%), Query Frame = 0


Query: 25  CAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLV 84
           CAHVMAMAVQKLFPDAKVTIGPWIENGF YDFD+EPLGDKDL KIKKEMERIIS NLPLV
Sbjct: 108 CAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLV 167

Query: 85  REEVSRDKA 93
           REEVSRD+A
Sbjct: 168 REEVSRDEA 176

[Close]

BLAST of CsGy4G024243 vs. TAIR 10
Match: AT2G04842.1 (threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative )

HSP 1 Score: 125.9 bits (315), Expect = 1.7e-29
Identity = 58/69 (84.06%), Postives = 65/69 (94.20%), Query Frame = 0


Query: 25  CAHVMAMAVQKLFPDAKVTIGPWIENGFCYDFDIEPLGDKDLMKIKKEMERIISTNLPLV 84
           CAHVMAMAVQKLFPDAKVTIGPWI+NGF YDFD+EPL DKDL +IKKEM+RIIS NLPL+
Sbjct: 86  CAHVMAMAVQKLFPDAKVTIGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLL 145

Query: 85  REEVSRDKA 94
           REEVSR++A
Sbjct: 146 REEVSREEA 154

The following BLAST results are available for this feature:

BLAST of CsGy4G024243 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IFC5	2.3e-28	84.06	Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX...	[more]
Q8DGW0	1.2e-19	65.22	Threonine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 ...	[more]
Q55806	1.5e-19	65.22	Threonine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=111170...	[more]
Q31JY3	3.4e-19	68.12	Threonine--tRNA ligase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) ...	[more]
Q5N1X6	3.4e-19	68.12	Threonine--tRNA ligase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG...	[more]

BLAST of CsGy4G024243 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8649928.1	5.95e-40	100.00	hypothetical protein Csa_012436 [Cucumis sativus]	[more]
XP_022847786.1	6.94e-35	84.51	threonine--tRNA ligase, chloroplastic/mitochondrial 2-like isoform X1 [Olea euro...	[more]
KAG6589116.1	1.75e-34	92.75	Threonine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyro...	[more]
KAG7011877.1	1.79e-34	92.75	Threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita argyrosperma su...	[more]
XP_004144536.1	1.79e-34	92.75	threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucumis sativus] >KGN4343...	[more]

BLAST of CsGy4G024243 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0Y5	2.76e-63	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G650170 PE=4 SV=1	[more]
A0A6J1JP39	8.69e-35	92.75	Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111486433 PE=3 SV=1	[more]
A0A0A0K179	8.69e-35	92.75	Threonyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_7G033430 PE=3 SV=1	[more]
A0A6J1GM45	8.69e-35	92.75	Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1	[more]
A0A6J1EKH5	8.69e-35	92.75	Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435298 PE=3 SV=1	[more]

BLAST of CsGy4G024243 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04842.1	1.7e-29	84.06	threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.980.10		coord: 25..78 e-value: 5.5E-22 score: 80.8
None	No IPR available	GENE3D	3.30.54.20		coord: 79..93 e-value: 5.5E-22 score: 80.8
None	No IPR available	PANTHER	PTHR11451:SF44	THREONINE--TRNA LIGASE, CHLOROPLASTIC/MITOCHONDRIAL 2	coord: 7..93
IPR002320	Threonine-tRNA ligase, class IIa	PANTHER	PTHR11451	THREONINE-TRNA LIGASE	coord: 7..93
IPR018163	Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily	SUPERFAMILY	55186	ThrRS/AlaRS common domain	coord: 25..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy4G024243.1	CsGy4G024243.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006435	threonyl-tRNA aminoacylation
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004829	threonine-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding