CsGy4G022820 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy4G022820
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Cytochrome P450 81E8-like
Location	Gy14Chr4: 29129322 .. 29132194 (-)
RNA-Seq Expression	CsGy4G022820
Synteny	CsGy4G022820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACCTCACACACTTACTTGTCTTTCTCTTTCTCTTCTCTTGATCAGTACTCTCATTCTTCTTTCCAAGCTCAGAAAAGTGAGAAACCAAAAAAAACTCCCACCAACCCCACCTTCTCTTCCCATAATCGGTCACCTCCATCTCCTCAAACAACCTTTCTACCGAGTACTACACGATCTCTCCAACAAATACGGTCCAATTCTATCCCTCACAATCGGCTCCCGTCCCGTGGTAGTCGTCTCCTCCCCCACTGCCGTCCGTGAATGCTTCACCAAAAACGACATCGTATTTGCCAACCGCCCTCGCTTACTCTCAGGAAAATACATCAATTACAACTACACCGCCATGGGATTTGCACCGTACGGGCAGCACTGGCGCAACATGCGCCGCATCGCCACAACAGAGCTACTCTCCAATCACCGGCTCAACACGTATCTCAACATTCGAGTGGAAGAACTGAAGTTATGGGTAAAGAATAGTTTGTACAAATGGAGTAGTGGGCGGGGGGATTTTGTTGTGGTGGATATGAAGTACAAATTGAAGGAACTTTCATTCAATACGGTGATGAGGATGATCAGTGGGAAAAGGTATTACGGTGTGGAAGTGGAGGATGTGGAAGAAGCATTGGAATTTAGAGAGATAATGAAAGAGCTACTTGAACTGAGTGGGGCTACTAATGCGGCAGATTTTTTTACAATTTTGAGAGTGTTCGATATTGAAGGAGTTAAGAAGAAGATGATGAAAGCGAGTGGGAGAGCAGATGTTTTTCTACAGAAACTTATTGATGAAGAGAGAGAAAAGGGAGTTTCACGTTGGCCGGAGGAAAAACAGAAAGAGGGGAAAACGTCTATGATTCGAACTTTGCTTTCCTTGCAGGAATCTCAACCTCAGTATTACTCCGATGATATAATCAAGGGTCATGTTCTGGTAAGTAGCCGTAACCTCCTTAGAAATAACAACTTCACTTTTAACCCTTTTCATATATAATTTAGAACTTAGTATCCAAATCGAAATGAGTTGAATGAAAAACGAGAACTATATTGAGTTGACAACTGATCATCGCTCCAAATATAATTTATATGGTTTAAATTACACCATTTCCACACTTCTCCTCTTACAAATAAAATAAGAGTTTCCAAAAAAAATAAAAAATATATAGATGCACGTATATATTTTATAACCAAGCATGTTATGATTAGATGCTAACTTAATCTATATGCATCCTTTTCTTACTACAAAAAGAAAAATAAAGATTCAATTTTAATATCAATTTAGCATGATATCGGAAAGAAAGATAAAAAAAATCAGATTTTGATGATCAAAATTCCTGTTATATAAAAAAAAAATCTCCTTTAATATTGATTTTTACCTATTAGACATTCTATTAAATTTTAAGGCTTATTGCTACAATCAAACGATAAAATAAATTTGGTGGAATGCAAAAAAAATACGAACATCATGTACTAAGTATTTTTTAATAAATTTAATCAAATTTACCATAATTACTACTACTTGGAGATTTTGGATTGATGGGTAGTTTAACCTGAAAAGTGATTTTACTTCATTTTAGCAATACTCTAAACATATAAGTATCTTAATTTATTGTTGGCTATAAAATATATTTAAAAATGGAGTATAATCTATTGATTAAAGGGGAATATTCTTCTTCTTATTGTTTTTTAGAACAGGTCGTTTCCAAATATGTGAAAAATGAACTAAAATAATTTAAAAAATATAGTACAATTTTTATCAATGATAAAGAGTTACATTTCTAAATAAAGTCAAAGATAATTCAAATAGTAAGTATCTTATTATCTTTTAACATTGGAGTTCTAAAATACATATGGATTTGTTGTTATTGTGTTGTAAGATAATAATATAAATTGCGGTGTCACTCTTGGACGAGAATATCAACACCTATCAATGCGGCAGAAACCCCCAAACAGACTTATCTATCCACTTGTCTTCACCATGAATAACAACACTCCATGTACTATCTCCTAATCCACCACATGGCCGAAATTCCTTCCTTTTGATTCATACTATATAAAATCATCAATCTGTGAACTGAAACAATAATGTAATTTAAAGTATTTGTTTCTGAATTTTATATAGAAATAGTAATAAGTAATATTTAAGCTCATAACTTAAATGGTGATAATCACAGTTTCAATATAATCCTCTAACCATTTAATTTTGTTGAACTTTATGATGTAATAGTTTAACTTAAATTTGTTATGTGTGTACAGACGATGTTGGCTGCAGGTACAGATACAACTTCAGGAACAATAGAATGGGCAATGTCACTACTTTTGAATCATCCAATGGTGATGGAGAAAGCTTGGATCGAGATAAGAGACTGTATCGGAGAAAATCAAATGGTGGAGGAAGGTGATGTGAGTAAACTAAAATATTTAGAAGCTATAATATACGAGACTCTTAGAATGTTTCCAGCAGCTCCATTGTTGGTTCCACACGAATGCAGTGAGGATTGTAGTATTGAAGGATTTGAGATACCCAAAGGAACGATGTTGATGGTGAATGCATGGGCCATTCATAGGGATCCGAAAGTGTGGGAGGATCCCACCAGCTTTCGACCGGAAAGGTTTCTAAATTGGGAAGGTGTCGAATCGTACAAGTGGATACCTTTTGGAATGGGGAGGAGGGCTTGTCCAGGGTTTGCTCTGGCCCAACGATCAATGGGATTGACATTAGCAACTCTGATTCAGTGCTTTGAGTGGGAGAAAGTTGATGAAAATGAACAAATAGACTTGTCTGAAGGGAGTGGAATCACTATGCCAAAAGCGAAAGCTTTGGAGGCAATGTGTAAACCTCGTAACTCAATGCTTCACTTGCTTGCTCAGCTTTGA

mRNA sequence

ATGGAACCTCACACACTTACTTGTCTTTCTCTTTCTCTTCTCTTGATCAGTACTCTCATTCTTCTTTCCAAGCTCAGAAAAGTGAGAAACCAAAAAAAACTCCCACCAACCCCACCTTCTCTTCCCATAATCGGTCACCTCCATCTCCTCAAACAACCTTTCTACCGAGTACTACACGATCTCTCCAACAAATACGGTCCAATTCTATCCCTCACAATCGGCTCCCGTCCCGTGGTAGTCGTCTCCTCCCCCACTGCCGTCCGTGAATGCTTCACCAAAAACGACATCGTATTTGCCAACCGCCCTCGCTTACTCTCAGGAAAATACATCAATTACAACTACACCGCCATGGGATTTGCACCGTACGGGCAGCACTGGCGCAACATGCGCCGCATCGCCACAACAGAGCTACTCTCCAATCACCGGCTCAACACGTATCTCAACATTCGAGTGGAAGAACTGAAGTTATGGGTAAAGAATAGTTTGTACAAATGGAGTAGTGGGCGGGGGGATTTTGTTGTGGTGGATATGAAGTACAAATTGAAGGAACTTTCATTCAATACGGTGATGAGGATGATCAGTGGGAAAAGGTATTACGGTGTGGAAGTGGAGGATGTGGAAGAAGCATTGGAATTTAGAGAGATAATGAAAGAGCTACTTGAACTGAGTGGGGCTACTAATGCGGCAGATTTTTTTACAATTTTGAGAGTGTTCGATATTGAAGGAGTTAAGAAGAAGATGATGAAAGCGAGTGGGAGAGCAGATGTTTTTCTACAGAAACTTATTGATGAAGAGAGAGAAAAGGGAGTTTCACGTTGGCCGGAGGAAAAACAGAAAGAGGGGAAAACGTCTATGATTCGAACTTTGCTTTCCTTGCAGGAATCTCAACCTCAGTATTACTCCGATGATATAATCAAGGGTCATGTTCTGACGATGTTGGCTGCAGGTACAGATACAACTTCAGGAACAATAGAATGGGCAATGTCACTACTTTTGAATCATCCAATGGTGATGGAGAAAGCTTGGATCGAGATAAGAGACTGTATCGGAGAAAATCAAATGGTGGAGGAAGGTGATGTGAGTAAACTAAAATATTTAGAAGCTATAATATACGAGACTCTTAGAATGTTTCCAGCAGCTCCATTGTTGGTTCCACACGAATGCAGTGAGGATTGTAGTATTGAAGGATTTGAGATACCCAAAGGAACGATGTTGATGGTGAATGCATGGGCCATTCATAGGGATCCGAAAGTGTGGGAGGATCCCACCAGCTTTCGACCGGAAAGGTTTCTAAATTGGGAAGGTGTCGAATCGTACAAGTGGATACCTTTTGGAATGGGGAGGAGGGCTTGTCCAGGGTTTGCTCTGGCCCAACGATCAATGGGATTGACATTAGCAACTCTGATTCAGTGCTTTGAGTGGGAGAAAGTTGATGAAAATGAACAAATAGACTTGTCTGAAGGGAGTGGAATCACTATGCCAAAAGCGAAAGCTTTGGAGGCAATGTGTAAACCTCGTAACTCAATGCTTCACTTGCTTGCTCAGCTTTGA

Coding sequence (CDS)

ATGGAACCTCACACACTTACTTGTCTTTCTCTTTCTCTTCTCTTGATCAGTACTCTCATTCTTCTTTCCAAGCTCAGAAAAGTGAGAAACCAAAAAAAACTCCCACCAACCCCACCTTCTCTTCCCATAATCGGTCACCTCCATCTCCTCAAACAACCTTTCTACCGAGTACTACACGATCTCTCCAACAAATACGGTCCAATTCTATCCCTCACAATCGGCTCCCGTCCCGTGGTAGTCGTCTCCTCCCCCACTGCCGTCCGTGAATGCTTCACCAAAAACGACATCGTATTTGCCAACCGCCCTCGCTTACTCTCAGGAAAATACATCAATTACAACTACACCGCCATGGGATTTGCACCGTACGGGCAGCACTGGCGCAACATGCGCCGCATCGCCACAACAGAGCTACTCTCCAATCACCGGCTCAACACGTATCTCAACATTCGAGTGGAAGAACTGAAGTTATGGGTAAAGAATAGTTTGTACAAATGGAGTAGTGGGCGGGGGGATTTTGTTGTGGTGGATATGAAGTACAAATTGAAGGAACTTTCATTCAATACGGTGATGAGGATGATCAGTGGGAAAAGGTATTACGGTGTGGAAGTGGAGGATGTGGAAGAAGCATTGGAATTTAGAGAGATAATGAAAGAGCTACTTGAACTGAGTGGGGCTACTAATGCGGCAGATTTTTTTACAATTTTGAGAGTGTTCGATATTGAAGGAGTTAAGAAGAAGATGATGAAAGCGAGTGGGAGAGCAGATGTTTTTCTACAGAAACTTATTGATGAAGAGAGAGAAAAGGGAGTTTCACGTTGGCCGGAGGAAAAACAGAAAGAGGGGAAAACGTCTATGATTCGAACTTTGCTTTCCTTGCAGGAATCTCAACCTCAGTATTACTCCGATGATATAATCAAGGGTCATGTTCTGACGATGTTGGCTGCAGGTACAGATACAACTTCAGGAACAATAGAATGGGCAATGTCACTACTTTTGAATCATCCAATGGTGATGGAGAAAGCTTGGATCGAGATAAGAGACTGTATCGGAGAAAATCAAATGGTGGAGGAAGGTGATGTGAGTAAACTAAAATATTTAGAAGCTATAATATACGAGACTCTTAGAATGTTTCCAGCAGCTCCATTGTTGGTTCCACACGAATGCAGTGAGGATTGTAGTATTGAAGGATTTGAGATACCCAAAGGAACGATGTTGATGGTGAATGCATGGGCCATTCATAGGGATCCGAAAGTGTGGGAGGATCCCACCAGCTTTCGACCGGAAAGGTTTCTAAATTGGGAAGGTGTCGAATCGTACAAGTGGATACCTTTTGGAATGGGGAGGAGGGCTTGTCCAGGGTTTGCTCTGGCCCAACGATCAATGGGATTGACATTAGCAACTCTGATTCAGTGCTTTGAGTGGGAGAAAGTTGATGAAAATGAACAAATAGACTTGTCTGAAGGGAGTGGAATCACTATGCCAAAAGCGAAAGCTTTGGAGGCAATGTGTAAACCTCGTAACTCAATGCTTCACTTGCTTGCTCAGCTTTGA

Protein sequence

MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHDLSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFAPYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYKLKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDIEGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDVSKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWEDPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDENEQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL*

Homology

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BLAST of CsGy4G022820 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 7.3e-148
Identity = 277/512 (54.10%), Postives = 366/512 (71.48%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKQPFYRVLH 60
           ME  TL    L+++L+S  I L      R ++ LPP+P  +LP+IGHLHL+ +  +R L+
Sbjct: 1   MESSTLLYTFLAVVLLS--ISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLY 60

Query: 61  DLSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGF 120
           DLS KYG + SL +G+R V+VVSSP A  ECFTKNDIVFANRP  + GKYI YNYT M  
Sbjct: 61  DLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVG 120

Query: 121 APYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKY 180
           +PYG+HWRN+RR+A  E+ S   LN +L+IR +E+K  +  SLY+ SSG+ DF  V+MK 
Sbjct: 121 SPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLL-SLYQ-SSGQ-DFGKVEMKS 180

Query: 181 KLKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFD 240
           KL ELSFN  MRM++GKRY+G +V D +EA  FR ++ E+ E +GA+N  DF   LR  D
Sbjct: 181 KLSELSFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWID 240

Query: 241 IEGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQY 300
            +  +KK+ K S   D FLQ+LI E R             +   +MI  LLSLQESQP+Y
Sbjct: 241 FKNYEKKVSKISQEMDAFLQRLIHESR-----------INKNNVTMIDHLLSLQESQPEY 300

Query: 301 YSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGD 360
           Y+D IIKG ++ +L AGTDT++ T+EWAMSLLLNHP  +EKA  EI   +G N+++EE D
Sbjct: 301 YTDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQD 360

Query: 361 VSKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVW 420
           + KL YL  II ET R+ PAAP+LVPHE S+DC ++G+++PKGT+L+VNAWAIHRDP+ W
Sbjct: 361 LPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFW 420

Query: 421 EDPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDE 480
           ++PT F+PER    E +E  K +PFGMGRR+CPG  LAQR +GLTL  LIQCFEW+++ E
Sbjct: 421 DEPTLFKPERHGGVE-LEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGE 480

Query: 481 NEQIDLSEGSGITMPKAKALEAMCKPRNSMLH 512
             +ID++EGSG+TMPKA+ LEA+CKPRN +LH
Sbjct: 481 -AKIDMAEGSGLTMPKAQPLEALCKPRN-ILH 492

[Close]

BLAST of CsGy4G022820 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.6e-142
Identity = 270/503 (53.68%), Postives = 346/503 (68.79%), Query Frame = 0


Query: 9   LSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHDLSNKYGPI 68
           +SL  L+I+  +  +  RK +N   LPP P  LPIIG+LH LKQP +   H LS KYG I
Sbjct: 10  ISLFFLIITLKVFFNTSRKFKN---LPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQI 69

Query: 69  LSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFAPYGQHWRN 128
            SL  GSR VVVVSS T  +ECFTKNDIV ANRP  L+GKYI YN T +  +PYG HWRN
Sbjct: 70  FSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRN 129

Query: 129 MRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYKLKELSFNT 188
           +RRI + E+LS+HRLN++L IR +E+   ++    K  +G   F  V+++    E++FNT
Sbjct: 130 LRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNG---FTEVELRPMFSEMTFNT 189

Query: 189 VMRMISGKRYYG--VEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDIEGVKKK 248
           +MRM+SGKRYYG   +V DVEEA  FR I+KE++ L GA N  DF   LR FD +G++K+
Sbjct: 190 IMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKR 249

Query: 249 MMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYYSDDIIK 308
           + K S R D FLQ LIDE R            K    +MI  LL+ Q+SQP+YY+D IIK
Sbjct: 250 LKKISKRTDAFLQGLIDEHR----------FGKRNSNTMIDHLLTQQQSQPEYYTDQIIK 309

Query: 309 GHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDVSKLKYL 368
           G ++ ML AGTDT+S TIEWAMS LLNHP +M+KA  E+   IG ++ V+E D+SKL YL
Sbjct: 310 GLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYL 369

Query: 369 EAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWEDPTSFR 428
           ++I+YETLR+  AAPLLVPH  SED S+ G+ IP+ T+LMVNAW IHRDP +W DPT F+
Sbjct: 370 QSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFK 429

Query: 429 PERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDENEQIDLS 488
           PERF   EG E  K + FG+GRRACPG  L+QR+ GLTL  LIQCFEW+++ E E+ID+ 
Sbjct: 430 PERF-EKEG-EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGE-EKIDMV 489

Query: 489 EGSGITMPKAKALEAMCKPRNSM 510
           E  GIT  K  +L AMCK R+ +
Sbjct: 490 EAKGITAGKKTSLNAMCKVRHPL 493

[Close]

BLAST of CsGy4G022820 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 2.5e-140
Identity = 262/497 (52.72%), Postives = 342/497 (68.81%), Query Frame = 0


Query: 12  SLLLISTLILLSKLRKV--RNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHDLSNKYGPIL 71
           SLL +S +I +  L K+  R  K LPP PP++PIIG+LH LK P +R    LS  YG I 
Sbjct: 6   SLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIF 65

Query: 72  SLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFAPYGQHWRNM 131
           SL  GSR VVVVSSP+   ECFTKNDI+ ANRPR L+GKYI YNYT +G A YG HWRN+
Sbjct: 66  SLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNL 125

Query: 132 RRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYKLKELSFNTV 191
           RRI T ++LSN+RLN++L +R +E    ++  L    S    F  V+++ +L E++FN +
Sbjct: 126 RRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAM 185

Query: 192 MRMISGKRYYG--VEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDIEGVKKKM 251
           MRMISGKRYYG   +V DVEEA +FREI+ E++ L GA N  DF  +LRV D++ ++K+ 
Sbjct: 186 MRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRC 245

Query: 252 MKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYYSDDIIKG 311
            + + R++ FL+ LI+E R   +         +G T MI  LL L ESQP+YYSD +IKG
Sbjct: 246 KRIAKRSNAFLEGLIEEHRRGNI-------HSDGGT-MIDHLLKLSESQPEYYSDHLIKG 305

Query: 312 HVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDVSKLKYLE 371
            +  ML AGTDT++ TIEW MS LLNHP V++KA  E+   IG+N++V+E D+SKL YL+
Sbjct: 306 LIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQ 365

Query: 372 AIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWEDPTSFRP 431
            II ETLR+ P APLL+PH  SEDC+I  F +PK T+++ N W IHRDPK W D  SF+P
Sbjct: 366 NIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKP 425

Query: 432 ERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDENEQIDLSE 491
           ERF   E V   K + FG+GRRACPG +LAQR++G T+  LIQCFEWE+  E E++D+ E
Sbjct: 426 ERFEKEEEVN--KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESE-EKLDMME 485

Query: 492 GSGITMPKAKALEAMCK 505
           G GITMP    L AMCK
Sbjct: 486 GKGITMPMKIPLRAMCK 491

[Close]

BLAST of CsGy4G022820 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 484.2 bits (1245), Expect = 1.9e-135
Identity = 244/503 (48.51%), Postives = 349/503 (69.38%), Query Frame = 0


Query: 6   LTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHDLSNKY 65
           L+ LS S+  ++   + + + + R  K LPP PPSLPIIG+LH LK+P +R    LS KY
Sbjct: 4   LSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKY 63

Query: 66  GPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFAPYGQH 125
           G + SL  GSR VVVVSS +  ++CFTKND+V ANRPR LSGKYI YNYT +G   YG+H
Sbjct: 64  GHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEH 123

Query: 126 WRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYKLKELS 185
           WRN+RRI   ++LSNHR+N++  IR +E +  +       S+   +F  +++  +L +++
Sbjct: 124 WRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSST---NFAEMELSSRLYDMT 183

Query: 186 FNTVMRMISGKRYYG--VEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDIEGV 245
           FN +MRMISGKRYYG   +  D++EA +FR+++ ELL+LSGA N  DF  +LR  D E +
Sbjct: 184 FNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENL 243

Query: 246 KKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYYSDD 305
           +K++   SG+ D FL+ LI+E R K          KE   +MI  LL+LQ+SQP+YY+D 
Sbjct: 244 EKRLKDISGKTDAFLRGLIEEHRTK----------KERANTMIDHLLNLQDSQPEYYTDQ 303

Query: 306 IIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDVSKL 365
           IIKG  L ML AGTD+++ T+EW+MS LLNHP V++K   E+   +G++++V+E D+ KL
Sbjct: 304 IIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKL 363

Query: 366 KYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWEDPT 425
            YL+ +I ETLR++  APLL+PH  S++C+I G+++P+ T++++NAWAIHRDP++W + T
Sbjct: 364 TYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEAT 423

Query: 426 SFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDENEQI 485
           +F+PERF     +E  K I FGMGRRACPG  LA R++ +TLA LIQCF+W K+   ++I
Sbjct: 424 TFKPERFEKKGELE--KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDW-KLINGDKI 483

Query: 486 DLSEGSGITMPKAKALEAMCKPR 507
           DL+E  G T+ K   L+AMCK R
Sbjct: 484 DLAERDGFTLTKLVPLKAMCKSR 490

[Close]

BLAST of CsGy4G022820 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 6.0e-134
Identity = 243/503 (48.31%), Postives = 352/503 (69.98%), Query Frame = 0


Query: 6   LTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHDLSNKY 65
           L+ L  SL  +S   ++  L + R  K LPP P SLPIIG+LH LK+P  R    L+ KY
Sbjct: 4   LSYLCYSLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKY 63

Query: 66  GPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFAPYGQH 125
           G ++SL  GSR VVVVSS +  +ECFTKND+V ANRPR LSGKYI YNYT +G   YG+H
Sbjct: 64  GNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEH 123

Query: 126 WRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYKLKELS 185
           WRN+RRI + ++LSNHR+N +  IR +E +  +K  L + SS +  F  V++ ++  +++
Sbjct: 124 WRNLRRITSLDVLSNHRINNFAPIRRDETQRLIK-KLAEDSSTK--FAEVELTFRFFDMT 183

Query: 186 FNTVMRMISGKRYYG--VEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDIEGV 245
           FN +MRMISGKRYYG   ++ +V+EA +FR+++ ELL+LSGA N  DF  +L+  D E +
Sbjct: 184 FNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENL 243

Query: 246 KKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYYSDD 305
           +K++ +   + DVFL  L+ E+R K          KE   +MI  LL++QESQP+YY+D 
Sbjct: 244 EKRVKRIGEKNDVFLSGLLQEQRSK----------KERTNTMIDHLLNMQESQPEYYTDT 303

Query: 306 IIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDVSKL 365
           IIKG  L ML AGTD+++ T+EW MS +LN+P V++K   E+   +G++++V+E D+ KL
Sbjct: 304 IIKGLCLAMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKL 363

Query: 366 KYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWEDPT 425
            YL  +IYETLR++  APLL+PH  +++C + G+++P+ T++++NAWAIHRDP+ W + T
Sbjct: 364 TYLRNVIYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEAT 423

Query: 426 SFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDENEQI 485
           +F+PERF + +G E  K I FGMGRRACPG  LA R++ +TLA L+QCF+W+++++ E+I
Sbjct: 424 TFKPERF-DKKG-ELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRIND-EKI 483

Query: 486 DLSEGSGITMPKAKALEAMCKPR 507
           D+SE  G TM K   L+AMCK R
Sbjct: 484 DMSERDGFTMTKLLPLKAMCKTR 490

[Close]

BLAST of CsGy4G022820 vs. NCBI nr
Match: XP_004141393.1 (cytochrome P450 81Q32 [Cucumis sativus] >KAE8649872.1 hypothetical protein Csa_012081 [Cucumis sativus])

HSP 1 Score: 1036 bits (2678), Expect = 0.0
Identity = 513/516 (99.42%), Postives = 515/516 (99.81%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60
           MEPH LTCLSLSLLLISTLILLSKLRK+RNQ+KLPPTPPSLPIIGHLHLLKQPFYRVLHD
Sbjct: 1   MEPHALTCLSLSLLLISTLILLSKLRKLRNQRKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60

Query: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120
           LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA
Sbjct: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120

Query: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180
           PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK
Sbjct: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180

Query: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240
           LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI
Sbjct: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240

Query: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300
           EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY
Sbjct: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300

Query: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 360
           SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV
Sbjct: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 360

Query: 361 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 420
           SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE
Sbjct: 361 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 420

Query: 421 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 480
           DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN
Sbjct: 421 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 480

Query: 481 EQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516
           EQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL
Sbjct: 481 EQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516

[Close]

BLAST of CsGy4G022820 vs. NCBI nr
Match: KAA0064424.1 (cytochrome P450 81E8-like [Cucumis melo var. makuwa] >TYK20163.1 cytochrome P450 81E8-like [Cucumis melo var. makuwa])

HSP 1 Score: 971 bits (2511), Expect = 0.0
Identity = 478/516 (92.64%), Postives = 500/516 (96.90%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60
           MEPHT+T  SLSLLLI+TLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLK+PF+RVLH+
Sbjct: 9   MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHN 68

Query: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120
           LSNKYGPILSLTIGSRPV+VVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTA+GFA
Sbjct: 69  LSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFA 128

Query: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180
           PYGQHWRNMRRIATTELLSNHRLN YLNIRVEELKLWVKN+LYKW SG GDFVVVDMK K
Sbjct: 129 PYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSG-GDFVVVDMKSK 188

Query: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240
           LKELSFNTVMRMISGKRY+GVEVEDVEEA EFREIMKELLELSGATNAADF  ILRVFD+
Sbjct: 189 LKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDL 248

Query: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300
           EGVKK+MMKASGRADVFLQKLIDEER+K VSRWPEEKQK+ KTSMIRTLLSLQESQPQYY
Sbjct: 249 EGVKKRMMKASGRADVFLQKLIDEERKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYY 308

Query: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 360
           SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPM M+KAWIEIRDC+GENQMVEEGDV
Sbjct: 309 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDV 368

Query: 361 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 420
           S+LKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFE+PKGTML+VNAW IHRDPKVWE
Sbjct: 369 SELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWE 428

Query: 421 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 480
           DPTSF+PERFLNWEGVE+YKWIPFGMGRRACPG ALAQRSMGLTLATLIQCFEW+KVDE 
Sbjct: 429 DPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQ 488

Query: 481 EQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516
           EQIDLSEGSGITMPKAKALEAMCKPR SMLHLLAQL
Sbjct: 489 EQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 523

[Close]

BLAST of CsGy4G022820 vs. NCBI nr
Match: XP_008453067.1 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 963 bits (2490), Expect = 0.0
Identity = 476/516 (92.25%), Postives = 498/516 (96.51%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60
           MEPHT+T  SLSLLLI+TLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLK+PF+RVLH+
Sbjct: 6   MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHN 65

Query: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120
           LSNKYGPILSLTIGSRPV+VVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTA+GFA
Sbjct: 66  LSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFA 125

Query: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180
           PYGQHWRNMRRIATTELLSNHRLN YLNIRVEELKLWVKN+LYKW SG GDFVVVDMK K
Sbjct: 126 PYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSG-GDFVVVDMKSK 185

Query: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240
           LKELSFNTVMRMISGKRY+GVEVEDVEEA EFREIMKELLELSGATNAADF  ILRVFD+
Sbjct: 186 LKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDL 245

Query: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300
           EGVKK+MMKASGRADVFLQKLIDEER+K   RWPEEKQK+ KTSMIRTLLSLQESQPQYY
Sbjct: 246 EGVKKRMMKASGRADVFLQKLIDEERKKEF-RWPEEKQKQAKTSMIRTLLSLQESQPQYY 305

Query: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 360
           SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPM M+KAWIEIRDC+GENQMVEEGDV
Sbjct: 306 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDV 365

Query: 361 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 420
           S+LKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFE+PKGTML+VNAW IHRDPKVWE
Sbjct: 366 SELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWE 425

Query: 421 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 480
           DPTSF+PERFLNWEGVE+YKWIPFGMGRRACPG ALAQRSMGLTLATLIQCFEW+KVDE 
Sbjct: 426 DPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQ 485

Query: 481 EQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516
           EQIDLSEGSGITMPKAKALEAMCKPR SMLHLLAQL
Sbjct: 486 EQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 519

[Close]

BLAST of CsGy4G022820 vs. NCBI nr
Match: XP_038896686.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 862 bits (2227), Expect = 8.87e-313
Identity = 432/516 (83.72%), Postives = 469/516 (90.89%), Query Frame = 0


Query: 1   MEPHTLTCLS--LSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVL 60
           MEPHT+T     L  L + T ILLSKLRK+ N KKLPP+PPSLP+IGHLHLL+ P +RVL
Sbjct: 1   MEPHTITWFFPFLISLFLFTFILLSKLRKI-NHKKLPPSPPSLPVIGHLHLLRDPLHRVL 60

Query: 61  HDLSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMG 120
           H+LSNKYGPILSL+IGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTA+G
Sbjct: 61  HNLSNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG 120

Query: 121 FAPYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMK 180
           FAPYGQHWRNMRRIATTELLSNHRLNTYLNIRV+ELKLW+KN LYK      +FV V+MK
Sbjct: 121 FAPYGQHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWIKN-LYKLICREDNFVGVEMK 180

Query: 181 YKLKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVF 240
            KLKELSFN VMRM+SGKRY+GVEV DVEEALEFRE+ KELLELSGATN ADF TILRVF
Sbjct: 181 SKLKELSFNIVMRMVSGKRYFGVEVVDVEEALEFRELTKELLELSGATNPADFLTILRVF 240

Query: 241 DIEGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQ 300
           D +G+ K+M+KASGRADVFLQKLIDE+RE+ +SRWPE+K   GK SMI+TLLSLQESQP 
Sbjct: 241 DFQGLNKRMLKASGRADVFLQKLIDEQRERRLSRWPEDKH--GK-SMIQTLLSLQESQPD 300

Query: 301 YYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEG 360
           YYSDDIIKGHVLTMLAAGTDTTSGTIEW MSLLLNHP VM+KAWIEIRDC+GEN+MVEE 
Sbjct: 301 YYSDDIIKGHVLTMLAAGTDTTSGTIEWTMSLLLNHPTVMKKAWIEIRDCVGENRMVEEA 360

Query: 361 DVSKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKV 420
           DVS L+Y+EAIIYETLR+FPAAPLLVPHE SEDC IEGFE+PKGTML+VNAWAIHRDPKV
Sbjct: 361 DVSNLRYMEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEVPKGTMLLVNAWAIHRDPKV 420

Query: 421 WEDPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVD 480
           WEDP SFRPERFLNWEGVESYKWIPFGMGRRACPG ALAQR +GLTLATLIQCFEWEKVD
Sbjct: 421 WEDPNSFRPERFLNWEGVESYKWIPFGMGRRACPGAALAQRVVGLTLATLIQCFEWEKVD 480

Query: 481 ENEQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLA 514
           E +QIDLSEGSGITMPKAKAL AMCKPRNSMLHLL+
Sbjct: 481 E-QQIDLSEGSGITMPKAKALVAMCKPRNSMLHLLS 510

[Close]

BLAST of CsGy4G022820 vs. NCBI nr
Match: XP_023535658.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796 bits (2056), Expect = 1.73e-286
Identity = 394/516 (76.36%), Postives = 455/516 (88.18%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60
           ME  T+T LSLSLL   TLIL+ KLRK ++ +K PP+PPSLP+IGHLHLLKQPF+RVL D
Sbjct: 20  MEASTITWLSLSLLF--TLILIHKLRK-QSPRKCPPSPPSLPLIGHLHLLKQPFHRVLQD 79

Query: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120
           LSNK+GPILSL+IGSRP+VV+SSPTAVRECFTKNDI FANRPRLLSGKY+NYNYT + FA
Sbjct: 80  LSNKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAFA 139

Query: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180
            YG HWRNMRRIATTELLSNHRLNTYLNIRV+ELKLWVKN LY  +    DFV ++M+ K
Sbjct: 140 SYGHHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWVKN-LYGATGESSDFVRLEMQSK 199

Query: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240
           L ELSFNTVMRM+SGKRY+GV+VEDVEEA EFREIMKELL+LSGA+N ADF  ILR+FD 
Sbjct: 200 LNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLPILRLFDY 259

Query: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300
           +G++K+M+KASGRAD+FLQ LID ERE+  S WPEEK   G  +MI++LLSLQESQPQYY
Sbjct: 260 QGLEKRMVKASGRADLFLQNLIDCERERRASPWPEEKH--GNKTMIQSLLSLQESQPQYY 319

Query: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 360
           SDDIIKGHVLTMLAAGTDTT+GTIEWAMSLLLNHP +M KAW EIR+C+GE+++VEEGDV
Sbjct: 320 SDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEEGDV 379

Query: 361 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 420
           S LKY+EAIIYETLR+FP APLLVPHE SE+C IEGF+IPKGTML+VNAWAIHRDP++WE
Sbjct: 380 SSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWE 439

Query: 421 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 480
           DPTSFRPER LNWE  ES+KW+PFG+GRRACPG ALA R +GLTLATLIQCFEW++V E 
Sbjct: 440 DPTSFRPERLLNWESAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGE- 499

Query: 481 EQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516
           E +DLSEG+GIT+PKA  LEAMC+ R+SMLHLL+ L
Sbjct: 500 EPVDLSEGTGITIPKAIPLEAMCRARSSMLHLLSLL 528

[Close]

BLAST of CsGy4G022820 vs. ExPASy TrEMBL
Match: A0A5A7VB12 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002950 PE=3 SV=1)

HSP 1 Score: 971 bits (2511), Expect = 0.0
Identity = 478/516 (92.64%), Postives = 500/516 (96.90%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60
           MEPHT+T  SLSLLLI+TLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLK+PF+RVLH+
Sbjct: 9   MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHN 68

Query: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120
           LSNKYGPILSLTIGSRPV+VVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTA+GFA
Sbjct: 69  LSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFA 128

Query: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180
           PYGQHWRNMRRIATTELLSNHRLN YLNIRVEELKLWVKN+LYKW SG GDFVVVDMK K
Sbjct: 129 PYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSG-GDFVVVDMKSK 188

Query: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240
           LKELSFNTVMRMISGKRY+GVEVEDVEEA EFREIMKELLELSGATNAADF  ILRVFD+
Sbjct: 189 LKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDL 248

Query: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300
           EGVKK+MMKASGRADVFLQKLIDEER+K VSRWPEEKQK+ KTSMIRTLLSLQESQPQYY
Sbjct: 249 EGVKKRMMKASGRADVFLQKLIDEERKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYY 308

Query: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 360
           SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPM M+KAWIEIRDC+GENQMVEEGDV
Sbjct: 309 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDV 368

Query: 361 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 420
           S+LKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFE+PKGTML+VNAW IHRDPKVWE
Sbjct: 369 SELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWE 428

Query: 421 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 480
           DPTSF+PERFLNWEGVE+YKWIPFGMGRRACPG ALAQRSMGLTLATLIQCFEW+KVDE 
Sbjct: 429 DPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQ 488

Query: 481 EQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516
           EQIDLSEGSGITMPKAKALEAMCKPR SMLHLLAQL
Sbjct: 489 EQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 523

[Close]

BLAST of CsGy4G022820 vs. ExPASy TrEMBL
Match: A0A1S3BWG9 (LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493891 PE=3 SV=1)

HSP 1 Score: 963 bits (2490), Expect = 0.0
Identity = 476/516 (92.25%), Postives = 498/516 (96.51%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60
           MEPHT+T  SLSLLLI+TLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLK+PF+RVLH+
Sbjct: 6   MEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHN 65

Query: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120
           LSNKYGPILSLTIGSRPV+VVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTA+GFA
Sbjct: 66  LSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFA 125

Query: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180
           PYGQHWRNMRRIATTELLSNHRLN YLNIRVEELKLWVKN+LYKW SG GDFVVVDMK K
Sbjct: 126 PYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSG-GDFVVVDMKSK 185

Query: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240
           LKELSFNTVMRMISGKRY+GVEVEDVEEA EFREIMKELLELSGATNAADF  ILRVFD+
Sbjct: 186 LKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDL 245

Query: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300
           EGVKK+MMKASGRADVFLQKLIDEER+K   RWPEEKQK+ KTSMIRTLLSLQESQPQYY
Sbjct: 246 EGVKKRMMKASGRADVFLQKLIDEERKKEF-RWPEEKQKQAKTSMIRTLLSLQESQPQYY 305

Query: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 360
           SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPM M+KAWIEIRDC+GENQMVEEGDV
Sbjct: 306 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDV 365

Query: 361 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 420
           S+LKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFE+PKGTML+VNAW IHRDPKVWE
Sbjct: 366 SELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWE 425

Query: 421 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 480
           DPTSF+PERFLNWEGVE+YKWIPFGMGRRACPG ALAQRSMGLTLATLIQCFEW+KVDE 
Sbjct: 426 DPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQ 485

Query: 481 EQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516
           EQIDLSEGSGITMPKAKALEAMCKPR SMLHLLAQL
Sbjct: 486 EQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 519

[Close]

BLAST of CsGy4G022820 vs. ExPASy TrEMBL
Match: A0A6J1F8L9 (isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443310 PE=3 SV=1)

HSP 1 Score: 790 bits (2040), Expect = 1.13e-284
Identity = 391/516 (75.78%), Postives = 453/516 (87.79%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60
           ME +T+T LSLSLL   TL L+ KLRK ++ ++ PP+PPSLP+IGHLHLLKQPF+RVL D
Sbjct: 1   MEANTITWLSLSLLF--TLSLIYKLRK-QSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQD 60

Query: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120
           LS+K+GPILSL+IGSRP+VV+SSPTAVRECFTKNDI FANRPRLLSGKY+NYNYT +  A
Sbjct: 61  LSDKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAVA 120

Query: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180
            YG HWRNMRRIATTELLSNHRLN YLNIRV+ELKLWVKN LY  +    DFV ++M+ K
Sbjct: 121 SYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKN-LYGETGESSDFVRLEMQSK 180

Query: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240
           L ELSFNTVMRM+SGKRY+GV+VEDVEEA EFREIMKELL LSGA+N ADF  ILR+FD 
Sbjct: 181 LNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRLFDY 240

Query: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300
           +G++K+M+KASGRAD+FLQ LID ERE+  SRWPEEK   G  +MI++LLS QESQPQYY
Sbjct: 241 QGLEKRMVKASGRADLFLQNLIDCERERRASRWPEEKH--GNKTMIQSLLSFQESQPQYY 300

Query: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 360
           SDDIIKGHVLTMLAAGTDTT+GTIEWAMSLLLNHP +M+KAW EIR+C+GE+++VEEGDV
Sbjct: 301 SDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGDV 360

Query: 361 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 420
           S LKY+EAIIYETLR+FP APLLVPHE SE+C IEGF+IPKGTML+VNAWAIHRDP++WE
Sbjct: 361 SSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWE 420

Query: 421 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 480
           DPTSFRPERFLNWE  ES+KW+PFG+GRRACPG ALA R +GLTLATLIQCFEW++V E 
Sbjct: 421 DPTSFRPERFLNWEAAESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGE- 480

Query: 481 EQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516
           E IDLSEG+GITMPKA  L AMC+ R+SMLHLL+ L
Sbjct: 481 EPIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509

[Close]

BLAST of CsGy4G022820 vs. ExPASy TrEMBL
Match: A0A6J1IL26 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 SV=1)

HSP 1 Score: 780 bits (2014), Expect = 1.48e-280
Identity = 388/516 (75.19%), Postives = 453/516 (87.79%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60
           ME +T+TCLSLSLLL  TLIL+ KLRK ++ +  PP+PPSLP+IGHLHLLKQPF+RVL D
Sbjct: 12  MEANTITCLSLSLLL--TLILIYKLRK-QSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQD 71

Query: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120
           LSNK+GPILSL+IGSRP+VV+SSPTAVRECFTKNDI  ANRPRLL+GKY+NYNYT + FA
Sbjct: 72  LSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFA 131

Query: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180
            YG HWRNMRRIATTELLSNHRLN YLNIRV+ELKL VKN LY  +    DFV ++M+ K
Sbjct: 132 SYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKN-LYGATGESSDFVRLEMQSK 191

Query: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240
           L ELSFN VMRM+SGKRY+GV+VEDVEEA EFREIMKELL+L GA+N ADF  ILR+FD 
Sbjct: 192 LNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDY 251

Query: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300
           +G++K+M++ASGRAD FLQ LID ER++  SRWPEEK+  G  +MI++LLSLQESQPQ+Y
Sbjct: 252 QGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKR--GNKTMIQSLLSLQESQPQHY 311

Query: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 360
           SDDIIKGHVLTMLAAGTDTT+GT+EWAMSLLLNHP +M KAW EIR+C+GE+++VEEGDV
Sbjct: 312 SDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDV 371

Query: 361 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 420
           S LKYL AIIYETLR+FPAAPLLVPHE SE+C +EGF+IPKGTML+VNAWAIHRDP+VWE
Sbjct: 372 SSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWE 431

Query: 421 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 480
           DPTSFRPERFLNWE  ES+KW+PFG+GRRACPG ALA R +GLTLATLIQCFEW++V E 
Sbjct: 432 DPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGE- 491

Query: 481 EQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516
           E IDLS+G+GIT PKA  LEAMC+ R+SMLHLL+ L
Sbjct: 492 EPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 519

[Close]

BLAST of CsGy4G022820 vs. ExPASy TrEMBL
Match: A0A0A0L0E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641750 PE=3 SV=1)

HSP 1 Score: 609 bits (1570), Expect = 2.92e-216
Identity = 307/314 (97.77%), Postives = 312/314 (99.36%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60
           MEPH LTCLSLSLLLISTLILLSKLRK+RNQ+KLPPTPPSLPIIGHLHLLKQPFYRVLHD
Sbjct: 1   MEPHALTCLSLSLLLISTLILLSKLRKLRNQRKLPPTPPSLPIIGHLHLLKQPFYRVLHD 60

Query: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120
           LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA
Sbjct: 61  LSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFA 120

Query: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180
           PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK
Sbjct: 121 PYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYK 180

Query: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240
           LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI
Sbjct: 181 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 240

Query: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300
           EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY
Sbjct: 241 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 300

Query: 301 SDDIIKGHVLTMLA 314
           SDDIIKGHVL +++
Sbjct: 301 SDDIIKGHVLNLVS 314

[Close]

BLAST of CsGy4G022820 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 483.8 bits (1244), Expect = 1.7e-136
Identity = 252/515 (48.93%), Postives = 356/515 (69.13%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKQPFYRVLH 60
           ME  TL   S+  +++S + L+ KL++  N   LPP+P  SLP+IGHL LLK P +R   
Sbjct: 1   METKTL-IFSILFVVLSLIYLIGKLKRKPN---LPPSPAWSLPVIGHLRLLKPPIHRTFL 60

Query: 61  DLSNKY--GPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAM 120
            LS      PI SL +G+R V V SS +   ECFTKND+V ANRP  +  K++ Y+YT M
Sbjct: 61  SLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTM 120

Query: 121 GFAPYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDM 180
             A YG HWRN+RRI + E+ SNHRLN++L+IR +E++  V      +S    +FV VDM
Sbjct: 121 IAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQ---EFVKVDM 180

Query: 181 KYKLKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRV 240
           K  L +L+FN ++RM++GKRYYG  VED  EA   R+++ +++  +GA NA D+  +LR+
Sbjct: 181 KSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRL 240

Query: 241 FDIEGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQP 300
             +   + ++ K +GR D FLQ L+DE+RE           KE   +MI  LL+LQESQP
Sbjct: 241 --VSDYETRVKKLAGRLDEFLQGLVDEKRE----------AKEKGNTMIDHLLTLQESQP 300

Query: 301 QYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEE 360
            Y++D IIKG++L ++ AGTDT++ T+EWA+S +LNHP V+ KA  EI   IG +++++E
Sbjct: 301 DYFTDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDE 360

Query: 361 GDVSKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPK 420
            D+S L YL+ I+ ETLR++PAAP+L+PH  SEDC + G+++P+GT+L+ N WAIHRDP+
Sbjct: 361 SDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQ 420

Query: 421 VWEDPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKV 480
           +W+DP SF+PERF   EG E+ K +PFG+GRRACPG  LA R + LTL +LIQC EWEK+
Sbjct: 421 LWDDPMSFKPERF-EKEG-EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI 480

Query: 481 DENEQIDLSEGSGITMPKAKALEAMCKPRNSMLHL 513
              E++D+SEG G+TMPKAK LEAMC+ R S++ +
Sbjct: 481 --GEEVDMSEGKGVTMPKAKPLEAMCRARPSVVKI 492

[Close]

BLAST of CsGy4G022820 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 453.8 bits (1166), Expect = 1.9e-127
Identity = 242/504 (48.02%), Postives = 345/504 (68.45%), Query Frame = 0


Query: 12  SLLLIST-----LILLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKQPFYRVLHDLSNKY 71
           +L+LI T     L L+  + +++ +  LPP+P  +LP+IGHL LLK P +RV   +S   
Sbjct: 3   ALMLIFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSL 62

Query: 72  G--PILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFAPYG 131
           G  PI+SL +G+R + VVSS +   ECFTKND++ ANR   +S K+I+Y  + +  A Y 
Sbjct: 63  GDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYS 122

Query: 132 QHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDMKYKLKE 191
           +HWRN+RRI   E+ S HRLN++ +IR +E++  +   L   S G   F  V+MK    +
Sbjct: 123 EHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYG---FTKVEMKSMFSD 182

Query: 192 LSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDIEGV 251
           L+FN ++RM++GK YYG   ED  EA   R ++ E +  SG  NAAD+  IL    I   
Sbjct: 183 LTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTW--ITYS 242

Query: 252 KKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYYSDD 311
           + ++ K +GR D FLQ L+DE+R        E K+K+  T M+  LL LQE+QP+YY D 
Sbjct: 243 ETRIKKLAGRLDEFLQGLVDEKR--------EGKEKKENT-MVDHLLCLQETQPEYYMDR 302

Query: 312 IIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDVSKL 371
           IIKG +L+++A GTDTT+ T+EWA+S LLN+P V+ KA  EI   IG ++++EE D+  L
Sbjct: 303 IIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNL 362

Query: 372 KYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWEDPT 431
            YL+ I+ ETLR++PAAP+L+PH  S+DC + G+++P+GTML+ NAWAIHRDP +W+DPT
Sbjct: 363 PYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPT 422

Query: 432 SFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDENEQI 491
           SF+PERF   EG E+ K +PFG+GRRACPG  LAQR + L+L +LIQCFEWE++ E E++
Sbjct: 423 SFKPERF-EKEG-EAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGE-EEV 482

Query: 492 DLSEGSGITMPKAKALEAMCKPRN 508
           D++EG G+TMPKA+ LEAMC+ R+
Sbjct: 483 DMTEGPGLTMPKARPLEAMCRARD 489

[Close]

BLAST of CsGy4G022820 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 451.4 bits (1160), Expect = 9.5e-127
Identity = 249/510 (48.82%), Postives = 341/510 (66.86%), Query Frame = 0


Query: 1   MEPHTLTCLSLSLLLISTLILLSKLRKVRNQKKLPPTPP-SLPIIGHLHLLKQPFYRVLH 60
           ME  TL    L + L  T I    + +++ +  LPP+P  +LP+IGHL LLK P +RV  
Sbjct: 1   MEGQTLIFTFLFISLSLTFI----IGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFL 60

Query: 61  DLSNKYG--PILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAM 120
            +S   G  PI+SL +G+R V VVSS +   ECFTKND+V ANR   L+ K+I+Y  T +
Sbjct: 61  SVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTV 120

Query: 121 GFAPYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKWSSGRGDFVVVDM 180
             A YG HWRN+RRI   E+ S HRLN++ +IR +E+   +   L + SS   +F  V+M
Sbjct: 121 VTASYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIA-CLSRNSS--LEFTKVEM 180

Query: 181 KYKLKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRV 240
           K     L+FN ++RM++GK YYG   ED  EA   RE++ E +   GA N AD+  IL  
Sbjct: 181 KSMFSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTW 240

Query: 241 FDIEGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQP 300
             I G +K++ K + R D FLQ L+DE R        E K+K   T M+  LL LQE+QP
Sbjct: 241 --ITGSEKRIKKIASRLDEFLQGLVDERR--------EGKEKRQNT-MVDHLLCLQETQP 300

Query: 301 QYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEE 360
           +YY+D+IIKG +L+++ AGTDT++ T+EW +S LLNHP ++ KA  EI + +G N++VEE
Sbjct: 301 EYYTDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEE 360

Query: 361 GDVSKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPK 420
            D+S L YL+ I+ E+LR++PA+PLLVPH  SEDC + G+ +P+GTML+ NAWAIHRDPK
Sbjct: 361 SDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPK 420

Query: 421 VWEDPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKV 480
           +W+DPTSF+PERF   EG E+ K + FG+GRRACPG  LAQR   LT+ +LIQCFEWE++
Sbjct: 421 IWDDPTSFKPERF-EKEG-EAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERI 480

Query: 481 DENEQIDLSE-GSGITMPKAKALEAMCKPR 507
            E E++D++E G G+ MPKA  L AMCK R
Sbjct: 481 GE-EEVDMTEGGGGVIMPKAIPLVAMCKAR 489

[Close]

BLAST of CsGy4G022820 vs. TAIR 10
Match: AT4G37430.1 (cytochrome P450, family 91, subfamily A, polypeptide 2 )

HSP 1 Score: 449.9 bits (1156), Expect = 2.8e-126
Identity = 237/512 (46.29%), Postives = 352/512 (68.75%), Query Frame = 0


Query: 9   LSLSLLLISTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKQPFYRVLHDLSNKYGPI 68
           L L  L+IS   L SK ++      LPP PPS P +GHLHL+K P +R+L   SN+YGPI
Sbjct: 7   LPLLFLVISYKFLYSKTQRF----NLPPGPPSRPFVGHLHLMKPPIHRLLQRYSNQYGPI 66

Query: 69  LSLTIGSRPVVVVSSPTAVRECFT-KNDIVFANRPRLLSGKYINYNYTAMGFAPYGQHWR 128
            SL  GSR VVV++SP+  +E FT +NDIV ++RP  L+ KY+ YN+T +G APYG HWR
Sbjct: 67  FSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHWR 126

Query: 129 NMRRIATTELLSNHRLNTYLNIRVEE-LKLWVKNSLYKWSSGR-GDFVVVDMKYKLKELS 188
           N+RRI + E+LS+HRL  + +IR +E L++  + S Y  +S    DF  ++++  L +L+
Sbjct: 127 NLRRICSQEILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSDLT 186

Query: 189 FNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDIEGVK- 248
           FN ++RM++GKRYYG +V + EEA  F++++ ++   SGA ++AD+  IL++F   G K 
Sbjct: 187 FNNIVRMVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLPILKLF---GNKF 246

Query: 249 KKMMKASGRA-DVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYYSDD 308
           +K +KA G++ D  LQ+L+DE R          + KEG T M+  L+SLQ+ QP+YY+D 
Sbjct: 247 EKEVKAIGKSMDDILQRLLDECR----------RDKEGNT-MVNHLISLQQQQPEYYTDV 306

Query: 309 IIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDVSKL 368
           IIKG +++M+ AGT+T++ T+EWAM+ LL +P V+EKA  EI + IG++++++E D++ L
Sbjct: 307 IIKGLMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVL 366

Query: 369 KYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWEDPT 428
            YL+ ++ ET R+FP AP L+P   ++D  I G+++P+ T++MVNAWAIHRDP++WE+P 
Sbjct: 367 PYLQNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPE 426

Query: 429 SFRPERFLNWEGVE--SYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDENE 488
            F P+R+ +  G +   YK +PFG GRR CPG  L QR + L L +LIQCFEWE V + E
Sbjct: 427 KFNPDRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENV-KGE 486

Query: 489 QIDLSEGSGITMPKAKALEAMCKPRNSMLHLL 514
           ++D+SE +G+ M K   L AMC+PR  M  LL
Sbjct: 487 EMDMSESTGLGMRKMDPLRAMCRPRPIMSKLL 499

[Close]

BLAST of CsGy4G022820 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 449.5 bits (1155), Expect = 3.6e-126
Identity = 250/507 (49.31%), Postives = 337/507 (66.47%), Query Frame = 0


Query: 9   LSLSLLLISTLILLSKLRKVRNQK-KLPPTPP-SLPIIGHLHLLKQPFYRVLHDLSNKY- 68
           L LSL      + L  L   R +K  LPP+P    PIIGHLHLLK P +R    LS    
Sbjct: 13  LILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLN 72

Query: 69  -GPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAMGFAPYGQ 128
              I SL++GSR V VVSS     ECFTKND+V ANRP  L GK+I YN T M  A YG 
Sbjct: 73  NAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGD 132

Query: 129 HWRNMRRIATTELLSNHRLNTYLNIRVEELKLWV----KNSLYKWSSGRGDFVVVDMKYK 188
            WRN+RRI T E+ S+ RLN++++IR +E++  +    KNS +        FV V+MK  
Sbjct: 133 SWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQH-------GFVKVEMKPL 192

Query: 189 LKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTILRVFDI 248
              L+ N ++RM++GKR+YG   E+  EA   R+++ E++   GA NAAD+F ILR   +
Sbjct: 193 FMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRY--V 252

Query: 249 EGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQESQPQYY 308
              +K + K +GR D FLQ L++E+R + V         +G T MI  LLSLQE+QP YY
Sbjct: 253 TNYEKHVKKLAGRVDEFLQSLVNEKRVEKV---------KGNT-MIDHLLSLQETQPDYY 312

Query: 309 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMVEEGDV 368
           +D IIKG +L M+ AGTDT++GT+EWAMS LLNHP V+ KA  EI D IG +++VEE D+
Sbjct: 313 TDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDI 372

Query: 369 SKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRDPKVWE 428
            KL YL+ I+ ETLR++P AP+L+PH  SEDC ++G+++P+GT+++VNAWAIHRDPK+WE
Sbjct: 373 VKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWE 432

Query: 429 DPTSFRPERFLNWEGVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFEWEKVDEN 488
           +P  F+PERF   +  E  K +PFG+GRR+CPG  LAQR + L L +L+QCFEWE+V+E 
Sbjct: 433 EPEKFKPERF--EKKGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEE- 492

Query: 489 EQIDLSEG-SGITMPKAKALEAMCKPR 507
           + +D+ E   G TM KA +L+AMCK R
Sbjct: 493 KYLDMRESEKGTTMRKATSLQAMCKAR 497

The following BLAST results are available for this feature:

BLAST of CsGy4G022820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	7.3e-148	54.10	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ8	1.6e-142	53.68	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNQ9	2.5e-140	52.72	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
P93147	1.9e-135	48.51	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNR0	6.0e-134	48.31	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of CsGy4G022820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141393.1	0.0	99.42	cytochrome P450 81Q32 [Cucumis sativus] >KAE8649872.1 hypothetical protein Csa_0...	[more]
KAA0064424.1	0.0	92.64	cytochrome P450 81E8-like [Cucumis melo var. makuwa] >TYK20163.1 cytochrome P450...	[more]
XP_008453067.1	0.0	92.25	PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81E8-like [Cucumis melo]	[more]
XP_038896686.1	8.87e-313	83.72	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
XP_023535658.1	1.73e-286	76.36	isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsGy4G022820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VB12	0.0	92.64	Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3BWG9	0.0	92.25	LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1F8L9	1.13e-284	75.78	isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1IL26	1.48e-280	75.19	isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 ...	[more]
A0A0A0L0E6	2.92e-216	97.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641750 PE=3 SV=1	[more]

BLAST of CsGy4G022820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37370.1	1.7e-136	48.93	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37360.1	1.9e-127	48.02	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT4G37340.1	9.5e-127	48.82	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT4G37430.1	2.8e-126	46.29	cytochrome P450, family 91, subfamily A, polypeptide 2	[more]
AT3G28740.1	3.6e-126	49.31	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 304..321 score: 34.69 coord: 62..81 score: 42.87 coord: 367..385 score: 39.5 coord: 451..474 score: 35.26 coord: 324..350 score: 35.11 coord: 86..107 score: 26.62 coord: 441..451 score: 53.42 coord: 408..432 score: 44.02 coord: 184..202 score: 18.7
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 315..332 score: 42.33 coord: 442..451 score: 58.19 coord: 451..462 score: 33.13 coord: 368..379 score: 41.25
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 35..497 e-value: 2.4E-100 score: 336.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 27..512 e-value: 2.0E-130 score: 437.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 35..506
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 8..485 e-value: 3.6E-22 score: 76.8
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 27..513
None	No IPR available	PANTHER	PTHR47947:SF27	SUBFAMILY NOT NAMED	coord: 27..513
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 444..453

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy4G022820.2	CsGy4G022820.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen