CsGy4G017930 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G017930
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionRibonuclease
LocationGy14Chr4: 23161410 .. 23161803 (+)
RNA-Seq ExpressionCsGy4G017930
SyntenyCsGy4G017930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATTCTTCTTTCTTTTTTACCATTGTTTTATCTTTTGAGTCCATTTGTTATGTTGAAGGGGAGAACTATGTTGAAGGGGAGGAAGTGTCCAATCGTGCAACTGTTGAAAGCAAACAGAAAGAAGTGCTCAAGGTTTGCAACTTGGATGTGGATATTTCTGAGTAACTTTTACTTCTTGTTGTTTGCGAACCTTTTCTATTGTCACACTCAGGTAATTGTCACCGAGGTTTTGGATGGTGACAAGTTTTATGTTCAGACAATTCCGGACCAAACAGCCACTTCTCTACAGCAGCAACTAGAGACTTTGAATCTTCAAGAAGTCCCTTTAATCTGTGGTTTTAGTCCTAAAAAAGGTGATATCGTCCTAGCTCAATTTAGTTCAGACAACTAG

mRNA sequence

ATGTATTCTTCTTTCTTTTTTACCATTGTTTTATCTTTTGAGTCCATTTGTTATGTTGAAGGGGAGAACTATGTTGAAGGGGAGGAAGTGTCCAATCGTGCAACTGTTGAAAGCAAACAGAAAGAAGTGCTCAAGGTAATTGTCACCGAGGTTTTGGATGGTGACAAGTTTTATGTTCAGACAATTCCGGACCAAACAGCCACTTCTCTACAGCAGCAACTAGAGACTTTGAATCTTCAAGAAGTCCCTTTAATCTGTGGTTTTAGTCCTAAAAAAGGTGATATCGTCCTAGCTCAATTTAGTTCAGACAACTAG

Coding sequence (CDS)

ATGTATTCTTCTTTCTTTTTTACCATTGTTTTATCTTTTGAGTCCATTTGTTATGTTGAAGGGGAGAACTATGTTGAAGGGGAGGAAGTGTCCAATCGTGCAACTGTTGAAAGCAAACAGAAAGAAGTGCTCAAGGTAATTGTCACCGAGGTTTTGGATGGTGACAAGTTTTATGTTCAGACAATTCCGGACCAAACAGCCACTTCTCTACAGCAGCAACTAGAGACTTTGAATCTTCAAGAAGTCCCTTTAATCTGTGGTTTTAGTCCTAAAAAAGGTGATATCGTCCTAGCTCAATTTAGTTCAGACAACTAG

Protein sequence

MYSSFFFTIVLSFESICYVEGENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQEVPLICGFSPKKGDIVLAQFSSDN*
Homology
BLAST of CsGy4G017930 vs. ExPASy Swiss-Prot
Match: Q9FLT0 (Ribonuclease TUDOR 2 OS=Arabidopsis thaliana OX=3702 GN=TSN2 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 2.1e-22
Identity = 54/84 (64.29%), Postives = 67/84 (79.76%), Query Frame = 0

Query: 22  ENYVEGEEVSNRAT-VESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQ 81
           ENYVEGEEV N ++ VE++QKE LKV+VTEVL G +FYVQT+ DQ   S+Q QL  L+L+
Sbjct: 710 ENYVEGEEVVNGSSKVETRQKETLKVVVTEVLGGGRFYVQTVGDQKVASIQNQLAALSLK 769

Query: 82  EVPLICGFSPKKGDIVLAQFSSDN 105
           + P+I  F+PKKGDIVLAQFS DN
Sbjct: 770 DAPIIGSFNPKKGDIVLAQFSLDN 793

BLAST of CsGy4G017930 vs. ExPASy Swiss-Prot
Match: Q8VZG7 (Ribonuclease TUDOR 1 OS=Arabidopsis thaliana OX=3702 GN=TSN1 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.1e-21
Identity = 53/85 (62.35%), Postives = 67/85 (78.82%), Query Frame = 0

Query: 22  ENYVEGEEVS--NRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNL 81
           ENYVEGEEVS  N  TVE++QKE LKV+VTEVL G +FYVQ+  DQ   S+Q QL +L++
Sbjct: 713 ENYVEGEEVSNGNTNTVETRQKETLKVVVTEVLGGGRFYVQSAGDQKIASIQNQLASLSI 772

Query: 82  QEVPLICGFSPKKGDIVLAQFSSDN 105
           ++ P+I  F+PK+GDIVLAQFS DN
Sbjct: 773 KDAPIIGSFNPKRGDIVLAQFSLDN 797

BLAST of CsGy4G017930 vs. ExPASy Swiss-Prot
Match: Q9W0S7 (Staphylococcal nuclease domain-containing protein 1 OS=Drosophila melanogaster OX=7227 GN=Tudor-SN PE=1 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 7.7e-04
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 0

Query: 23  NYVEGEEVSNRATVESKQKEVL--------KVIVTEVLDGDKFYVQTIPDQTATSLQQQL 82
           NYVE        T E K+ +V+         VIVTE+ +   F+ Q++  ++ + L+  +
Sbjct: 674 NYVEEVPKEKTVTEEEKEDKVVAERKVNYENVIVTEITETLTFFAQSV--ESGSKLESLM 733

Query: 83  ETL--NLQEVPLICG-FSPKKGDIVLAQFSSDN 105
             L  + Q  P I G ++PK+GD+V AQF+ DN
Sbjct: 734 SKLHADFQSNPPIAGSYTPKRGDLVAAQFTLDN 764

BLAST of CsGy4G017930 vs. NCBI nr
Match: KAE8649665.1 (hypothetical protein Csa_012973 [Cucumis sativus])

HSP 1 Score: 203 bits (516), Expect = 1.21e-65
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 0

Query: 1   MYSSFFFTIVLSFESICYVEGENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQ 60
           MYSSFFFTIVLSFESICYVEGENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQ
Sbjct: 1   MYSSFFFTIVLSFESICYVEGENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQ 60

Query: 61  TIPDQTATSLQQQLETLNLQEVPLICGFSPKKGDIVLAQFSSDN 104
           TIPDQTATSLQQQLETLNLQEVPLICGFSPKKGDIVLAQFSSDN
Sbjct: 61  TIPDQTATSLQQQLETLNLQEVPLICGFSPKKGDIVLAQFSSDN 104

BLAST of CsGy4G017930 vs. NCBI nr
Match: KAG6589331.1 (Ribonuclease TUDOR 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 130 bits (327), Expect = 1.53e-34
Identity = 69/83 (83.13%), Postives = 71/83 (85.54%), Query Frame = 0

Query: 22  ENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQE 81
           ENYVEGEEV N A VESKQKEVLKVIVTEVL G KFYVQTI DQ  TSLQ+QL  LNLQE
Sbjct: 23  ENYVEGEEVPNGAAVESKQKEVLKVIVTEVLGGGKFYVQTIGDQKVTSLQKQLAALNLQE 82

Query: 82  VPLICGFSPKKGDIVLAQFSSDN 104
           VPLI  FSPKKGDIVLAQFS+DN
Sbjct: 83  VPLIGAFSPKKGDIVLAQFSADN 105

BLAST of CsGy4G017930 vs. NCBI nr
Match: XP_038878676.1 (ribonuclease TUDOR 1-like [Benincasa hispida] >XP_038878677.1 ribonuclease TUDOR 1-like [Benincasa hispida])

HSP 1 Score: 135 bits (340), Expect = 2.81e-34
Identity = 72/83 (86.75%), Postives = 73/83 (87.95%), Query Frame = 0

Query: 22  ENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQE 81
           ENYVEGEEVSN A VESKQKEVLKVIVTEVL G KFYVQTI DQ ATSLQQQL  LNLQE
Sbjct: 713 ENYVEGEEVSNGAAVESKQKEVLKVIVTEVLGGGKFYVQTIGDQKATSLQQQLAALNLQE 772

Query: 82  VPLICGFSPKKGDIVLAQFSSDN 104
           VPLI  FSPKKGDIVLAQFS+DN
Sbjct: 773 VPLIGAFSPKKGDIVLAQFSADN 795

BLAST of CsGy4G017930 vs. NCBI nr
Match: XP_008464356.1 (PREDICTED: staphylococcal nuclease domain-containing protein 1 [Cucumis melo] >ADN33852.1 short-chain dehydrogenase/reductase [Cucumis melo subsp. melo] >KAA0057721.1 staphylococcal nuclease domain-containing protein 1 [Cucumis melo var. makuwa] >TYJ98406.1 staphylococcal nuclease domain-containing protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 135 bits (340), Expect = 2.81e-34
Identity = 72/83 (86.75%), Postives = 73/83 (87.95%), Query Frame = 0

Query: 22  ENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQE 81
           ENYVEGEEVSN A VESKQKEVLKVIVTEVL G KFYVQTI DQ ATSLQQQL  LNLQE
Sbjct: 713 ENYVEGEEVSNGAAVESKQKEVLKVIVTEVLGGGKFYVQTIGDQKATSLQQQLAALNLQE 772

Query: 82  VPLICGFSPKKGDIVLAQFSSDN 104
           VPLI  FSPKKGDIVLAQFS+DN
Sbjct: 773 VPLIGAFSPKKGDIVLAQFSADN 795

BLAST of CsGy4G017930 vs. NCBI nr
Match: XP_004138013.1 (ribonuclease TUDOR 1 [Cucumis sativus] >KGN63427.1 hypothetical protein Csa_014157 [Cucumis sativus])

HSP 1 Score: 134 bits (337), Expect = 7.13e-34
Identity = 71/83 (85.54%), Postives = 73/83 (87.95%), Query Frame = 0

Query: 22  ENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQE 81
           ENYVEGEEVSN A VESKQKEVLKVIVTEVL G KFYVQTI DQ ATSLQQQL  LNLQE
Sbjct: 713 ENYVEGEEVSNGAAVESKQKEVLKVIVTEVLGGGKFYVQTIGDQKATSLQQQLAALNLQE 772

Query: 82  VPLICGFSPKKGDIVLAQFSSDN 104
           VPLI  F+PKKGDIVLAQFS+DN
Sbjct: 773 VPLIGAFNPKKGDIVLAQFSADN 795

BLAST of CsGy4G017930 vs. ExPASy TrEMBL
Match: A0A5D3BH85 (Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00020 PE=4 SV=1)

HSP 1 Score: 135 bits (340), Expect = 1.36e-34
Identity = 72/83 (86.75%), Postives = 73/83 (87.95%), Query Frame = 0

Query: 22  ENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQE 81
           ENYVEGEEVSN A VESKQKEVLKVIVTEVL G KFYVQTI DQ ATSLQQQL  LNLQE
Sbjct: 713 ENYVEGEEVSNGAAVESKQKEVLKVIVTEVLGGGKFYVQTIGDQKATSLQQQLAALNLQE 772

Query: 82  VPLICGFSPKKGDIVLAQFSSDN 104
           VPLI  FSPKKGDIVLAQFS+DN
Sbjct: 773 VPLIGAFSPKKGDIVLAQFSADN 795

BLAST of CsGy4G017930 vs. ExPASy TrEMBL
Match: E5GBL0 (Ribonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 135 bits (340), Expect = 1.36e-34
Identity = 72/83 (86.75%), Postives = 73/83 (87.95%), Query Frame = 0

Query: 22  ENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQE 81
           ENYVEGEEVSN A VESKQKEVLKVIVTEVL G KFYVQTI DQ ATSLQQQL  LNLQE
Sbjct: 713 ENYVEGEEVSNGAAVESKQKEVLKVIVTEVLGGGKFYVQTIGDQKATSLQQQLAALNLQE 772

Query: 82  VPLICGFSPKKGDIVLAQFSSDN 104
           VPLI  FSPKKGDIVLAQFS+DN
Sbjct: 773 VPLIGAFSPKKGDIVLAQFSADN 795

BLAST of CsGy4G017930 vs. ExPASy TrEMBL
Match: A0A1S3CL97 (Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103502266 PE=4 SV=1)

HSP 1 Score: 135 bits (340), Expect = 1.36e-34
Identity = 72/83 (86.75%), Postives = 73/83 (87.95%), Query Frame = 0

Query: 22  ENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQE 81
           ENYVEGEEVSN A VESKQKEVLKVIVTEVL G KFYVQTI DQ ATSLQQQL  LNLQE
Sbjct: 713 ENYVEGEEVSNGAAVESKQKEVLKVIVTEVLGGGKFYVQTIGDQKATSLQQQLAALNLQE 772

Query: 82  VPLICGFSPKKGDIVLAQFSSDN 104
           VPLI  FSPKKGDIVLAQFS+DN
Sbjct: 773 VPLIGAFSPKKGDIVLAQFSADN 795

BLAST of CsGy4G017930 vs. ExPASy TrEMBL
Match: A0A0A0LRJ8 (Ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_1G000020 PE=4 SV=1)

HSP 1 Score: 134 bits (337), Expect = 3.45e-34
Identity = 71/83 (85.54%), Postives = 73/83 (87.95%), Query Frame = 0

Query: 22  ENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQE 81
           ENYVEGEEVSN A VESKQKEVLKVIVTEVL G KFYVQTI DQ ATSLQQQL  LNLQE
Sbjct: 713 ENYVEGEEVSNGAAVESKQKEVLKVIVTEVLGGGKFYVQTIGDQKATSLQQQLAALNLQE 772

Query: 82  VPLICGFSPKKGDIVLAQFSSDN 104
           VPLI  F+PKKGDIVLAQFS+DN
Sbjct: 773 VPLIGAFNPKKGDIVLAQFSADN 795

BLAST of CsGy4G017930 vs. ExPASy TrEMBL
Match: A0A6J1E6F3 (ribonuclease TUDOR 1-like OS=Cucurbita moschata OX=3662 GN=LOC111429852 PE=4 SV=1)

HSP 1 Score: 130 bits (327), Expect = 5.57e-33
Identity = 69/83 (83.13%), Postives = 71/83 (85.54%), Query Frame = 0

Query: 22  ENYVEGEEVSNRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQE 81
           ENYVEGEEV N A VESKQKEVLKVIVTEVL G KFYVQTI DQ  TSLQ+QL  LNLQE
Sbjct: 371 ENYVEGEEVPNGAAVESKQKEVLKVIVTEVLGGGKFYVQTIGDQKVTSLQKQLAALNLQE 430

Query: 82  VPLICGFSPKKGDIVLAQFSSDN 104
           VPLI  FSPKKGDIVLAQFS+DN
Sbjct: 431 VPLIGAFSPKKGDIVLAQFSADN 453

BLAST of CsGy4G017930 vs. TAIR 10
Match: AT5G61780.1 (TUDOR-SN protein 2 )

HSP 1 Score: 106.3 bits (264), Expect = 1.5e-23
Identity = 54/84 (64.29%), Postives = 67/84 (79.76%), Query Frame = 0

Query: 22  ENYVEGEEVSNRAT-VESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNLQ 81
           ENYVEGEEV N ++ VE++QKE LKV+VTEVL G +FYVQT+ DQ   S+Q QL  L+L+
Sbjct: 710 ENYVEGEEVVNGSSKVETRQKETLKVVVTEVLGGGRFYVQTVGDQKVASIQNQLAALSLK 769

Query: 82  EVPLICGFSPKKGDIVLAQFSSDN 105
           + P+I  F+PKKGDIVLAQFS DN
Sbjct: 770 DAPIIGSFNPKKGDIVLAQFSLDN 793

BLAST of CsGy4G017930 vs. TAIR 10
Match: AT5G07350.1 (TUDOR-SN protein 1 )

HSP 1 Score: 104.0 bits (258), Expect = 7.6e-23
Identity = 53/85 (62.35%), Postives = 67/85 (78.82%), Query Frame = 0

Query: 22  ENYVEGEEVS--NRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNL 81
           ENYVEGEEVS  N  TVE++QKE LKV+VTEVL G +FYVQ+  DQ   S+Q QL +L++
Sbjct: 713 ENYVEGEEVSNGNTNTVETRQKETLKVVVTEVLGGGRFYVQSAGDQKIASIQNQLASLSI 772

Query: 82  QEVPLICGFSPKKGDIVLAQFSSDN 105
           ++ P+I  F+PK+GDIVLAQFS DN
Sbjct: 773 KDAPIIGSFNPKRGDIVLAQFSLDN 797

BLAST of CsGy4G017930 vs. TAIR 10
Match: AT5G07350.2 (TUDOR-SN protein 1 )

HSP 1 Score: 104.0 bits (258), Expect = 7.6e-23
Identity = 53/85 (62.35%), Postives = 67/85 (78.82%), Query Frame = 0

Query: 22  ENYVEGEEVS--NRATVESKQKEVLKVIVTEVLDGDKFYVQTIPDQTATSLQQQLETLNL 81
           ENYVEGEEVS  N  TVE++QKE LKV+VTEVL G +FYVQ+  DQ   S+Q QL +L++
Sbjct: 713 ENYVEGEEVSNGNTNTVETRQKETLKVVVTEVLGGGRFYVQSAGDQKIASIQNQLASLSI 772

Query: 82  QEVPLICGFSPKKGDIVLAQFSSDN 105
           ++ P+I  F+PK+GDIVLAQFS DN
Sbjct: 773 KDAPIIGSFNPKRGDIVLAQFSLDN 797

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLT02.1e-2264.29Ribonuclease TUDOR 2 OS=Arabidopsis thaliana OX=3702 GN=TSN2 PE=1 SV=1[more]
Q8VZG71.1e-2162.35Ribonuclease TUDOR 1 OS=Arabidopsis thaliana OX=3702 GN=TSN1 PE=1 SV=1[more]
Q9W0S77.7e-0433.33Staphylococcal nuclease domain-containing protein 1 OS=Drosophila melanogaster O... [more]
Match NameE-valueIdentityDescription
KAE8649665.11.21e-65100.00hypothetical protein Csa_012973 [Cucumis sativus][more]
KAG6589331.11.53e-3483.13Ribonuclease TUDOR 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038878676.12.81e-3486.75ribonuclease TUDOR 1-like [Benincasa hispida] >XP_038878677.1 ribonuclease TUDOR... [more]
XP_008464356.12.81e-3486.75PREDICTED: staphylococcal nuclease domain-containing protein 1 [Cucumis melo] >A... [more]
XP_004138013.17.13e-3485.54ribonuclease TUDOR 1 [Cucumis sativus] >KGN63427.1 hypothetical protein Csa_0141... [more]
Match NameE-valueIdentityDescription
A0A5D3BH851.36e-3486.75Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00020 P... [more]
E5GBL01.36e-3486.75Ribonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3CL971.36e-3486.75Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103502266 PE=4 SV=1[more]
A0A0A0LRJ83.45e-3485.54Ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_1G000020 PE=4 SV=1[more]
A0A6J1E6F35.57e-3383.13ribonuclease TUDOR 1-like OS=Cucurbita moschata OX=3662 GN=LOC111429852 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT5G61780.11.5e-2364.29TUDOR-SN protein 2 [more]
AT5G07350.17.6e-2362.35TUDOR-SN protein 1 [more]
AT5G07350.27.6e-2362.35TUDOR-SN protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR12302EBNA2 BINDING PROTEIN P100coord: 21..104
NoneNo IPR availablePANTHERPTHR12302:SF20RIBONUCLEASEcoord: 21..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G017930.2CsGy4G017930.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031047 gene silencing by RNA
biological_process GO:0006402 mRNA catabolic process
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
cellular_component GO:0016442 RISC complex
molecular_function GO:0004518 nuclease activity
molecular_function GO:0003723 RNA binding