CsGy4G017300 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G017300
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description(1->3)-beta-glucan endohydrolase
LocationGy14Chr4: 22321436 .. 22326234 (-)
RNA-Seq ExpressionCsGy4G017300
SyntenyCsGy4G017300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCTTATTCTTCCCTACAACTTCCCTTTTGTTCATCTTCATATTTTCCCATCAATTCCTTCATAGAAGTAATTAACAAATCCTAAAGTTTTATATCCACAAATTTTGTTAATTTGTTCCTTTTTAATACGTTGGAATTTTGTGTTACAGGTGTGTTTGGCGCAGTTGGAGTGAACTATGGGTTGAATAGCGACAATCTTCCAAAACCAAATGAAGTGATAAACTTATACGAACGATGTGGGATAAATATTGTTCGGATATTCGAGCCCAACCATGAGATTCTTCATGCTTTGTGTGGCAAGGAAAATTTAGTGTTGTGGCTAGGTACAAGGAATGAAGACATTGAGGGGTTTGCTACAAATCAAGAAGTGGCTAATGCTTGGGTTAATGCTAATGTGGTTCGTTATTACAAGGATGTCAATATTGCTTATATCACTGTTGGCAATGAAGTTGTCCCTGGCGATGCAGCTTCACCATTTGTTGCTAATGCCATTAAAAATATGATGCAAGCGCTTGATAATGCGGGCGTCCAAAGCGACATTAAGGTAATGAATGCTTTATTGAATTAAATTATGAATTTGGTTTTGTTACATTTTTCATTTGATTGGATATTGTTTTGATTTCTTCGTTACTATTATTTTGACTTTGGAATACACTTAAAAGGGTTAGGATTTCATAAGAGATTAAATAAAAATGATTTTTTGTACTGAGGTATGTTTCAAAGTTTTTATAAATTAATAATAATAATAATAATAAAAGTAATTTTGAGTGGTAGCAGATTTAATTTGATTAATGAGATTTGGATTTAGATTCATTTTATCCCTTATAAATCACTTGTAATGTGAATAATATTTGAGTTAGGTTTTCTATATATAGTCTAATCTTTATAATTTTAAAATTTTGGATTCGTATTCTATATTTGGACTTGGTGTGAACGCCATTTTTCAAAAGGAATAATGCGTAACTTATATGATCTATTAATTCCAAGTCCAAGTACACAAAAGAATTTAGTTTTTAGTAAATTAAACATTTACAGGTGACAACAGTGGTAGCCATGACAGTATTGGAAGTGTCATCCCCACCTTCGGCCGGGGCATTCTCAGCAATCGCCGCTAGAACAATGAAAGACATCGGAAATGTGTTAGAATCTTCATGTGCACCAATATTGGTGAATGTGTACCCATATTTCGCTTACGCATCAAACCCACAACAAATATCAATGAGTTACGCATTGTTCACATCCACAAGCCCGGTAGTAGTAGATGGAGATTTGCAGTACTTCAATTTGTTGGATGCCATGGTGGATTCATTCTATGCAGCGCTGGAGAAGATTGGCGTTGAGGGAGTGAGAATCGGGATATCAGAGACTGGGTGGCCCACCAAAGGAAACGAGCCATTCACGAGCGTTGAAAATGCACTGACTTATAACAAAAATATTGTGGAACATGTGAGCAGTGGAGTTGGAACCCCCAGAATGCCCAATCTCCAATATGACGTTGTTTTGTTTGAAATGTTCAATGAGGATCTTAAGTCGCCTGGAGTTGAGCAGAATTTTGGCTTCTTTGATCCCAGTATGAATCCAGTTTATTCTTTTTGGAATTGTTGAATAACCCATCTCCCCTCCCTACCAAAATCTTCATTTTCCATTGTATATTACCTACTACCTTCACCTTTAATGGTTACGGTTCTTTTTTAACTTTATAAATGTACTTCCCATTTAACTAATATTTCATCAATGTTGGTCACGTTTTAAAAATATGAGGGATATATTGCTGTTTCATTCCTTGTTTATACTCCATATATGTACCATTATTTACTAAAATACTTACCAAATATGTTTTATTACTATATATATATATATATATGTTCATTAAAAAGAATATTCATTTTTTAAAATAATTGAAACTATTTATAAAATATTATAACTATCTTCCATCCTTGTCAGGTCCTTCAAATTATTCAAATTATTTTTAGAAACTAAATTTAAGATCTATTAAAATGATGTTGAGGTCCTTCAAATTCTTTTTAGATTCTAAATTTAAGATCTATTCAAACTATATGAACTCAAATCGAAAAGAATGTGTATTCAAACTATAGGAACTCAAATCGAAAAGAATGTGAACCACAGTGATATTTTATTCTTACTTTTGTTTTTGTGAGCTTATAAATCCTAAATTCTGAGAAAAAAATATATTTTTTCATTTACAAGAGTTGTATTTATACTTAACAAGATAACTACTCAATCTACGGTATCTTGCTTATATTTAGCACATATTTAAACTACTTCAAAACATAAAAAATTCTTTGATCAAATCTTTAACAACTATTTTACTCATTCTCTCACCCGTTTTAATTACTAACTTATTTACTCTAAGAAAAATTATTTTTACAAATAACTTAACAAATTAACCAATTCAAAAAGTGGTGTATCTTATCCATGATGATAATAGTAATAATAAGAGAAAATATTAAGTAACGTAATAAGAGTTGGTTGATGAGTGTTTTAGATTGTAGAGGGGATGAGAGTGTTATGCACAATAATGAAGAGAAAAGAGTAGAATAATTCTTTTATTTGTTTCTGTTCTTTTTCCTCTGTCTCCATTTGGTAACATAATATATATATATATATATATATATATATATATATATATGTACATTTCCAATTGATTACCTCACAATCGTTTTCTGTTCAAAACAGAAAATATAAAAGACAAAAAAAACATCCCATAAAACTCATCTAATATTCGTTAATAACAAAGAATACCTAAATATATTTAAAAGAAGAAAAAAAAAGAATCTAATTGTCCAAAAAATGACAAATCAAAATGGCCACATTATCATTAGCTAGTCCGGAGTCATTAACCGACACGTGCCGACATGCGTGAATGTTTGTTAAGTACACTCAAATATTATATAGAATGCCCACCTCCTATTTTACCTTTCTGTTTCCTTAAACGGAATTACCAAATTCCCATATTCAATTCCCTATAAATTTTCTTCATTTTCCCATTGCTTATCACAGAAAAAGAAATACCATCATTCACACCCCATGGACTTCTTCTTTCCTACTTCCTTTCTTCTGTTGCTCTCTCTCTTTTCTCATCAAATCCCTCCTATAAGTACTTCTACATGTTGTAACTTTGTTTTCTTTCTCTATTCCTTTCTTCTTTACCTTCTTTGTTTTGATGTGTTCAACTTTGTGTTCTAGGTGGCTTCGGCACCCTTGGAGTCAACTATGGGTTGAATGGCGATAATCTTCCAACACCGAGTGATGTCATAAACCTATACGGACGATGTGGGATTAATATTCTACGGTTATTTGAGCCCAACCATGGGGTTCTTGATGCTTTGCAAGGTAAGAAAGATTTAGTGTTGTGGCTAGGGACGAGAAATGAAGACATACAGGGGTTTGCTACAAACCAATTAGCCGCAAATGCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGAATGTCAACATTGCTTATATCACTATTGGCAATGAAGTTGTTCCTGGTGATGCAGCTGCACCTTTTGTTGCCAATGGCATTAAAAATATAATGCAGGCCCTTGTCGACGTCGGCATTAAAAGCGACATTAAGGTGTGACAATCTATTGGGGTTAAATTACAAATTTAGTTTCTATATGATTTTTTTTTTTATTGGTATATTATTTGAAGAATTTTATAATTTTCAACTTTTTCATAAATTAAAAAAAAAGAGAGACAAGTTTTGGTTTCATTTTGTCCCCTAAAAATTTAGAGTTGTAGTTTGTAAATATATATCTAAGTTAGTTTTTCCATACATAGACCATTTTTACTTAAAAGTAACTATATATGTATATGTATAGTCTTTTAACCTTTATTGTCTTAAAATTTTCCATTAGCTTTCTATTTTCAGATTTTTTTGTCAACATGGCTGAGAACGAGGTTATAGGTTTGATTTTAAAAAATAAAAAAAAATAAAATGTTTACTATAGACGTTAATTATTGAAAATTCAATCTTAATGACATACAAGTTAGATCTAAAAGTATAAAATTAAATGTTAAATTAATTTTGAGTGATAAAAAGGGTGTATGATTATAATGATTTTAAAGCTGAGAGAAGTTATTTTAATTTAAAATTTTAAAATTATTCACAAAGAAACATGTATTAATTAATTAAGATCTTAGAAATCAAATAGGTAGAAGTATATAGTATGATTTTGAGTAAATTAAAAATGTGCAGGTGACAACAGTAGTAGCCATGACAGCATTGGGAGTGTCATACCCCCCTTCAGCGGGAGCGTTCACAGGCAGTGCTGCTGGAATAATGAAAGACATCGCAAATGTGTTAGGATCTTCGGGGGCACCAATATTGGTGAATGTGTACCCATATTTTGCCTACGCATCAAACCCACAACAAATCTCACTCAATTACGCACTCTTCACATCCTCTACCCCTGTTGTGGTTGATGGAAATTTACAGTACTTCAATCTGTTCGACGCTATGGTGGATTCATTCTATGCAGCTCTGGAGAAGATTGACGCTGGAGAAATAAGAATTGGGATATCAGAGACTGGGTGGCCCACCAATGGAAACGAACCATTCACGAGCGTTGAGAATGCATTGACTTATAACAAAAACCTTGTGAAACATGTCACAAGTGGAGTTGGAACCCCCAAAAGGCCCAATCTCAAATATGATGTCGTTTTGTTCGAAATGTTCAATGAGGATCTTAAAGCACCTGGAGTTGAGCAAAATTTTGGCTTTTTTTCCCCTAATATGAACCCAGTTTATCCCTTCTGGAATTGTTGA

mRNA sequence

ATGCCCTTATTCTTCCCTACAACTTCCCTTTTGTTCATCTTCATATTTTCCCATCAATTCCTTCATAGAAGTGTGTTTGGCGCAGTTGGAGTGAACTATGGGTTGAATAGCGACAATCTTCCAAAACCAAATGAAGTGATAAACTTATACGAACGATGTGGGATAAATATTGTTCGGATATTCGAGCCCAACCATGAGATTCTTCATGCTTTGTGTGGCAAGGAAAATTTAGTGTTGTGGCTAGGTACAAGGAATGAAGACATTGAGGGGTTTGCTACAAATCAAGAAGTGGCTAATGCTTGGGTTAATGCTAATGTGGTTCGTTATTACAAGGATGTCAATATTGCTTATATCACTGTTGGCAATGAAGTTGTCCCTGGCGATGCAGCTTCACCATTTGTTGCTAATGCCATTAAAAATATGATGCAAGCGCTTGATAATGCGGGCGTCCAAAGCGACATTAAGGTGACAACAGTGGTAGCCATGACAGTATTGGAAGTGTCATCCCCACCTTCGGCCGGGGCATTCTCAGCAATCGCCGCTAGAACAATGAAAGACATCGGAAATGTGTTAGAATCTTCATGTGCACCAATATTGGTGAATGTGTACCCATATTTCGCTTACGCATCAAACCCACAACAAATATCAATGAGTTACGCATTGTTCACATCCACAAGCCCGGTAGTAGTAGATGGAGATTTGCAGTACTTCAATTTGTTGGATGCCATGGTGGATTCATTCTATGCAGCGCTGGAGAAGATTGGCGTTGAGGGAGTGAGAATCGGGATATCAGAGACTGGGTGGCCCACCAAAGGAAACGAGCCATTCACGAGCGTTGAAAATGCACTGACTTATAACAAAAATATTGTGGAACATGTGAGCAGTGGAGTTGGAACCCCCAGAATGCCCAATCTCCAATATGACGTTGTTTTGTTTGAAATGTTCAATGAGGATCTTAAGTCGCCTGGAGTTGAGCAGAATTTTGGCTTCTTTGATCCCAGTGGCTTCGGCACCCTTGGAGTCAACTATGGGTTGAATGGCGATAATCTTCCAACACCGAGTGATGTCATAAACCTATACGGACGATGTGGGATTAATATTCTACGGTTATTTGAGCCCAACCATGGGGTTCTTGATGCTTTGCAAGGTAAGAAAGATTTAGTGTTGTGGCTAGGGACGAGAAATGAAGACATACAGGGGTTTGCTACAAACCAATTAGCCGCAAATGCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGAATGTCAACATTGCTTATATCACTATTGGCAATGAAGTTGTTCCTGGTGATGCAGCTGCACCTTTTGTTGCCAATGGCATTAAAAATATAATGCAGGCCCTTGTCGACGTCGGCATTAAAAGCGACATTAAGGTGACAACAGTAGTAGCCATGACAGCATTGGGAGTGTCATACCCCCCTTCAGCGGGAGCGTTCACAGGCAGTGCTGCTGGAATAATGAAAGACATCGCAAATGTGTTAGGATCTTCGGGGGCACCAATATTGGTGAATGTGTACCCATATTTTGCCTACGCATCAAACCCACAACAAATCTCACTCAATTACGCACTCTTCACATCCTCTACCCCTGTTGTGGTTGATGGAAATTTACAGTACTTCAATCTGTTCGACGCTATGGTGGATTCATTCTATGCAGCTCTGGAGAAGATTGACGCTGGAGAAATAAGAATTGGGATATCAGAGACTGGGTGGCCCACCAATGGAAACGAACCATTCACGAGCGTTGAGAATGCATTGACTTATAACAAAAACCTTGTGAAACATGTCACAAGTGGAGTTGGAACCCCCAAAAGGCCCAATCTCAAATATGATGTCGTTTTGTTCGAAATGTTCAATGAGGATCTTAAAGCACCTGGAGTTGAGCAAAATTTTGGCTTTTTTTCCCCTAATATGAACCCAGTTTATCCCTTCTGGAATTGTTGA

Coding sequence (CDS)

ATGCCCTTATTCTTCCCTACAACTTCCCTTTTGTTCATCTTCATATTTTCCCATCAATTCCTTCATAGAAGTGTGTTTGGCGCAGTTGGAGTGAACTATGGGTTGAATAGCGACAATCTTCCAAAACCAAATGAAGTGATAAACTTATACGAACGATGTGGGATAAATATTGTTCGGATATTCGAGCCCAACCATGAGATTCTTCATGCTTTGTGTGGCAAGGAAAATTTAGTGTTGTGGCTAGGTACAAGGAATGAAGACATTGAGGGGTTTGCTACAAATCAAGAAGTGGCTAATGCTTGGGTTAATGCTAATGTGGTTCGTTATTACAAGGATGTCAATATTGCTTATATCACTGTTGGCAATGAAGTTGTCCCTGGCGATGCAGCTTCACCATTTGTTGCTAATGCCATTAAAAATATGATGCAAGCGCTTGATAATGCGGGCGTCCAAAGCGACATTAAGGTGACAACAGTGGTAGCCATGACAGTATTGGAAGTGTCATCCCCACCTTCGGCCGGGGCATTCTCAGCAATCGCCGCTAGAACAATGAAAGACATCGGAAATGTGTTAGAATCTTCATGTGCACCAATATTGGTGAATGTGTACCCATATTTCGCTTACGCATCAAACCCACAACAAATATCAATGAGTTACGCATTGTTCACATCCACAAGCCCGGTAGTAGTAGATGGAGATTTGCAGTACTTCAATTTGTTGGATGCCATGGTGGATTCATTCTATGCAGCGCTGGAGAAGATTGGCGTTGAGGGAGTGAGAATCGGGATATCAGAGACTGGGTGGCCCACCAAAGGAAACGAGCCATTCACGAGCGTTGAAAATGCACTGACTTATAACAAAAATATTGTGGAACATGTGAGCAGTGGAGTTGGAACCCCCAGAATGCCCAATCTCCAATATGACGTTGTTTTGTTTGAAATGTTCAATGAGGATCTTAAGTCGCCTGGAGTTGAGCAGAATTTTGGCTTCTTTGATCCCAGTGGCTTCGGCACCCTTGGAGTCAACTATGGGTTGAATGGCGATAATCTTCCAACACCGAGTGATGTCATAAACCTATACGGACGATGTGGGATTAATATTCTACGGTTATTTGAGCCCAACCATGGGGTTCTTGATGCTTTGCAAGGTAAGAAAGATTTAGTGTTGTGGCTAGGGACGAGAAATGAAGACATACAGGGGTTTGCTACAAACCAATTAGCCGCAAATGCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGAATGTCAACATTGCTTATATCACTATTGGCAATGAAGTTGTTCCTGGTGATGCAGCTGCACCTTTTGTTGCCAATGGCATTAAAAATATAATGCAGGCCCTTGTCGACGTCGGCATTAAAAGCGACATTAAGGTGACAACAGTAGTAGCCATGACAGCATTGGGAGTGTCATACCCCCCTTCAGCGGGAGCGTTCACAGGCAGTGCTGCTGGAATAATGAAAGACATCGCAAATGTGTTAGGATCTTCGGGGGCACCAATATTGGTGAATGTGTACCCATATTTTGCCTACGCATCAAACCCACAACAAATCTCACTCAATTACGCACTCTTCACATCCTCTACCCCTGTTGTGGTTGATGGAAATTTACAGTACTTCAATCTGTTCGACGCTATGGTGGATTCATTCTATGCAGCTCTGGAGAAGATTGACGCTGGAGAAATAAGAATTGGGATATCAGAGACTGGGTGGCCCACCAATGGAAACGAACCATTCACGAGCGTTGAGAATGCATTGACTTATAACAAAAACCTTGTGAAACATGTCACAAGTGGAGTTGGAACCCCCAAAAGGCCCAATCTCAAATATGATGTCGTTTTGTTCGAAATGTTCAATGAGGATCTTAAAGCACCTGGAGTTGAGCAAAATTTTGGCTTTTTTTCCCCTAATATGAACCCAGTTTATCCCTTCTGGAATTGTTGA

Protein sequence

MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITVGNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIAARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLLDAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTPRMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPNMNPVYPFWNC*
Homology
BLAST of CsGy4G017300 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 305.1 bits (780), Expect = 2.0e-81
Identity = 152/317 (47.95%), Postives = 207/317 (65.30%), Query Frame = 0

Query: 333 PSGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLG 392
           P+   ++GV YG+ G+NLP PS+V++LY    I  +RL++PN   L AL+     VL L 
Sbjct: 23  PTRVQSIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVL-LD 82

Query: 393 TRNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIM 452
               D+Q  A+N  AA  W+  NVV Y+ +V+  YI +GNE++PG   A ++   ++NI 
Sbjct: 83  VPRSDVQSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIY 142

Query: 453 QALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNV 512
            AL   G+++ IKV+T V    LG SYPPSAGAF+ +A   +  I   L S+GAP+LVNV
Sbjct: 143 NALSSAGLQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNV 202

Query: 513 YPYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIG 572
           YPYF+Y  NP QISL YALFT+S  VV DG   Y NLFDA+VD+ +AALE++    + + 
Sbjct: 203 YPYFSYTGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVV 262

Query: 573 ISETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAP 632
           +SE+GWP+ G     S  NA TYN+NL++HV  G GTP+RP  + +  +FEMFNE+ KA 
Sbjct: 263 VSESGWPSAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAG 322

Query: 633 GVEQNFGFFSPNMNPVY 650
           G+EQNFG F PN  PVY
Sbjct: 323 GIEQNFGLFYPNKQPVY 336

BLAST of CsGy4G017300 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.1e-76
Identity = 152/324 (46.91%), Postives = 214/324 (66.05%), Query Frame = 0

Query: 334 SGFGT----LGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVL 393
           SGF T    +G+NYGL GDNLP+PS+VINLY   GI+ +R+F+PN  VL+AL+G +D+ +
Sbjct: 25  SGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEV 84

Query: 394 WLGTRNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIK 453
            +G +++D+   A ++ A   W  AN+  Y  +VNI +IT+GNEV+PG      +   + 
Sbjct: 85  TVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGP-----IGPQVL 144

Query: 454 NIMQALVDVGIKS---DIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGA 513
            +MQ+L ++ +KS    I ++TVVAM+ L  SYPPSAG FT  A   +  +  +L  +  
Sbjct: 145 PVMQSLTNL-VKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTST 204

Query: 514 PILVNVYPYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDA 573
           PILVN+YPYFAYAS+P  I L+YA F + + VV DG+L Y N+FDA+ D+F  A+EK   
Sbjct: 205 PILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGV 264

Query: 574 GEIRIGISETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFN 633
             + + +SETGWP+ GN  FT+   A TYN+N VKH+ SG GTPKRPN   +  LF  FN
Sbjct: 265 KNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFN 324

Query: 634 EDLKAPGVEQNFGFFSP-NMNPVY 650
           E+ K  G EQNFG ++P +M P+Y
Sbjct: 325 ENQKPAGTEQNFGLYNPSDMKPIY 342

BLAST of CsGy4G017300 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.6e-75
Identity = 151/345 (43.77%), Postives = 218/345 (63.19%), Query Frame = 0

Query: 309 VVLFEMFNEDLKSPGVEQNFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINIL 368
           VV+    N D  +     + GF + +   ++G+NYGL GDNLP+PS VI LY    I  +
Sbjct: 17  VVIVNYNNSDFVN--AANSIGFVNAA--NSIGLNYGLLGDNLPSPSKVITLYKSIDITKI 76

Query: 369 RLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYI 428
           R+F+PN  VL+AL+G +D+ + +G R++D+   + ++ A   W   N+ PY  ++NIA+I
Sbjct: 77  RIFDPNTEVLNALRGHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIAFI 136

Query: 429 TIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKS---DIKVTTVVAMTALGVSYPPSAGA 488
           T+GNEV+PG      +   +  +MQ+L ++ +KS    I ++TVVAM  L  SYPPSAG 
Sbjct: 137 TVGNEVIPGP-----IGPQVLPVMQSLTNL-VKSRNLPISISTVVAMWNLEQSYPPSAGM 196

Query: 489 FTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVVDGNLQ 548
           FT  A   +  +  +L  + +PILV +YPYF+YAS+P  I L+YA F +   VV DG+L 
Sbjct: 197 FTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGSLG 256

Query: 549 YFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLVKHVTS 608
           Y N+FDA+ D+F  A+EK    ++ + +SETGWP+ GN   T+ + A TYN+N VKH+ S
Sbjct: 257 YSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIAS 316

Query: 609 GVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPN-MNPVY 650
           G GTPKRPN   D  LF  FNE+ K  G EQNFG ++PN M P+Y
Sbjct: 317 GKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFGLYNPNDMKPIY 351

BLAST of CsGy4G017300 vs. ExPASy Swiss-Prot
Match: Q02439 (Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4513 PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 4.2e-68
Identity = 144/318 (45.28%), Postives = 202/318 (63.52%), Query Frame = 0

Query: 334 SGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGT 393
           +G   +GVNYG+ G +LP+P  V+ LY    I  +R+F P+  VL+AL+    L + LGT
Sbjct: 2   AGVEGIGVNYGMMGSDLPSPDKVVALYKANNITDVRIFHPDTNVLEALR-NSGLGVVLGT 61

Query: 394 RNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQ 453
            N D+   A++   A +WV++ V P+   V+  YI  GNEV+PG++AA  V   +KN+  
Sbjct: 62  LNSDLAPLASDASYAASWVHSYVQPFAGAVSFRYINAGNEVIPGESAA-LVLPAMKNLEA 121

Query: 454 ALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVY 513
           AL   G+   + VTT +A + LG SYPPS G F+ +A   +  I + L SSG P+LVNVY
Sbjct: 122 ALQAAGL--SVPVTTAMATSVLGTSYPPSQGTFSEAALPTVGPIVSHLASSGTPLLVNVY 181

Query: 514 PYFAYASNPQQISLNYALFTSSTPV-VVDGNLQYFNLFDAMVDSFYAALEKIDAGE-IRI 573
           PYFAY+++P  + L+YAL +SS  V V D  ++Y N+FDA++D+ YAA+EK   GE + +
Sbjct: 182 PYFAYSADPSSVRLDYALLSSSAAVAVTDNGVEYANMFDAILDAVYAAVEKAGGGESLEL 241

Query: 574 GISETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKA 633
            +SETGWP+ G     SVENA  Y  NLV+HV    GTP+RP    +  +F MFNE+ K 
Sbjct: 242 VVSETGWPSGGGGYGASVENAAAYINNLVRHVG---GTPRRPGKAVETYIFAMFNENQKP 301

Query: 634 PGVEQNFGFFSPNMNPVY 650
            GVEQNFG F P+M+ VY
Sbjct: 302 EGVEQNFGMFQPDMSQVY 312

BLAST of CsGy4G017300 vs. ExPASy Swiss-Prot
Match: Q01413 (Glucan endo-1,3-beta-glucosidase B OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.6e-67
Identity = 141/314 (44.90%), Postives = 197/314 (62.74%), Query Frame = 0

Query: 339 LGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDI 398
           +GV YG+ G+NLP+ S+VI LY    I  LRL++PNHG L+AL+G    V+ LG  N D+
Sbjct: 27  IGVCYGMMGNNLPSHSEVIQLYKSRNIRRLRLYDPNHGALNALRGSNIEVI-LGLPNVDV 86

Query: 399 QGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAA---APFVANGIKNIMQAL 458
           +  ++    A  WV  NV  ++ +V I YI +GNE+ P       APF    + NI +A+
Sbjct: 87  KHISSGMEHARWWVQKNVRDFWPHVKIKYIAVGNEISPVTGTSNLAPFQVPALVNIYKAI 146

Query: 459 VDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPY 518
            + G+ +DIKV+T V MT +G SYPPS G+F          I   L  + AP+LVN+YPY
Sbjct: 147 GEAGLGNDIKVSTSVDMTLIGNSYPPSQGSFRNDVRWFTDPIVGFLRDTRAPLLVNIYPY 206

Query: 519 FAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISE 578
           F+Y+ NP QISL YALFT+   VV DG+ QY NLFDAM+DS YAA+++   G + I +SE
Sbjct: 207 FSYSGNPGQISLPYALFTAPNVVVQDGSRQYRNLFDAMLDSVYAAMDRTGGGSVGIVVSE 266

Query: 579 TGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVE 638
           +GWP+ G    T  ENA TY +NL++H     G+P++P    +  +F MF+E+ K P +E
Sbjct: 267 SGWPSAGAFGATH-ENAQTYLRNLIQHAKE--GSPRKPG-PIETYIFAMFDENNKNPELE 326

Query: 639 QNFGFFSPNMNPVY 650
           ++FG FSPN  P Y
Sbjct: 327 KHFGMFSPNKQPKY 335

BLAST of CsGy4G017300 vs. NCBI nr
Match: KAE8649635.1 (hypothetical protein Csa_012736 [Cucumis sativus])

HSP 1 Score: 1307 bits (3382), Expect = 0.0
Identity = 654/654 (100.00%), Postives = 654/654 (100.00%), Query Frame = 0

Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI
Sbjct: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120
           FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV
Sbjct: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120

Query: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA
Sbjct: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180

Query: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240
           ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL
Sbjct: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300
           DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP
Sbjct: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLY 360
           RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLY
Sbjct: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLY 360

Query: 361 GRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYY 420
           GRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYY
Sbjct: 361 GRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYY 420

Query: 421 KNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVVAMTALGVSYP 480
           KNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVVAMTALGVSYP
Sbjct: 421 KNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVVAMTALGVSYP 480

Query: 481 PSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVV 540
           PSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVV
Sbjct: 481 PSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVV 540

Query: 541 DGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLV 600
           DGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLV
Sbjct: 541 DGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLV 600

Query: 601 KHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPNMNPVYPFWNC 654
           KHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPNMNPVYPFWNC
Sbjct: 601 KHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPNMNPVYPFWNC 654

BLAST of CsGy4G017300 vs. NCBI nr
Match: KAA0064262.1 (putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa])

HSP 1 Score: 1186 bits (3068), Expect = 0.0
Identity = 594/664 (89.46%), Postives = 626/664 (94.28%), Query Frame = 0

Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M LFFPTTSLL IFIFSHQ LH SVFGAVGVNYGLNSDNLPKP+EVINLYERCGINIVRI
Sbjct: 1   MALFFPTTSLLLIFIFSHQILHISVFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120
           FEPNHE+L AL  KENLVLWLGTRNEDI+GFATNQEVA+AWVNANVV YYKDVNIAYITV
Sbjct: 61  FEPNHEVLDALRCKENLVLWLGTRNEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITV 120

Query: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGDAA+PFVANAI+N+MQAL NA VQSDIKVTTVVAMT LEVSSPPSAGAF+AIA
Sbjct: 121 GNEVVPGDAATPFVANAIQNIMQALVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIA 180

Query: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240
           AR MKDIG+VLESSCAPIL+NVYPYFAYASNPQQI +SYALFTST+PVVVDGDL+YFNLL
Sbjct: 181 ARAMKDIGSVLESSCAPILMNVYPYFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300
           DAMVDSFY ALEKIGVEGVRIGISETGWP+KGNEPFTSV+NALTYN+N V+HVSSGVGTP
Sbjct: 241 DAMVDSFYTALEKIGVEGVRIGISETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS----------GFGTLGVNYGLNGDNL 360
           +MPNLQYD+VLFEMFNEDLKSPGVEQNFGFF P+          G GT+GVNYGLNGDNL
Sbjct: 301 KMPNLQYDIVLFEMFNEDLKSPGVEQNFGFFYPNMNPVYSFWNCGSGTIGVNYGLNGDNL 360

Query: 361 PTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANA 420
           P PSDVINLY RCGINI+RLFEPNHGVLDALQGKKDLVLWLGTRNEDI+GFATNQLAAN 
Sbjct: 361 PIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANT 420

Query: 421 WVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVV 480
           WVNANVVPYYK+VNI+YIT+GNEVVPGDAAAPFVAN IKNIMQALV++GIKSDIKVTTVV
Sbjct: 421 WVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVV 480

Query: 481 AMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYA 540
           AMT L VSYPPSAGAFT SAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYA
Sbjct: 481 AMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYA 540

Query: 541 LFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVE 600
           LFTS+TPVVVDG+LQYFNLFDAMVDSFYAALEKIDA EIRIGISETGWPT+GNEPFTSV+
Sbjct: 541 LFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGISETGWPTSGNEPFTSVD 600

Query: 601 NALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPNMNPVYP 654
           NALTYNKNLV HVTSGVGTPKRPNLKYD VLFEMFNEDLKAPGVEQNFGFF PNMNPVYP
Sbjct: 601 NALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGVEQNFGFFFPNMNPVYP 660

BLAST of CsGy4G017300 vs. NCBI nr
Match: XP_016899853.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo])

HSP 1 Score: 1144 bits (2958), Expect = 0.0
Identity = 578/654 (88.38%), Postives = 610/654 (93.27%), Query Frame = 0

Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M LFFPTTSLL IFIFSHQ LH SVFGAVGVNYGLNSDNLPKP+EVINLYERCGINIVRI
Sbjct: 1   MALFFPTTSLLLIFIFSHQILHISVFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120
           FEPNHE+L AL  KENLVLWLGTRNEDI+GFATNQEVA+AWVNANVV YYKDVNIAYITV
Sbjct: 61  FEPNHEVLDALRCKENLVLWLGTRNEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITV 120

Query: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGDAA+PFVANAI+N+MQAL NA VQSDIKVTTVVAMT LEVSSPPSAGAF+AIA
Sbjct: 121 GNEVVPGDAATPFVANAIQNIMQALVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIA 180

Query: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240
           AR MKDIG+VLESSCAPIL+NVYPYFAYASNPQQI +SYALFTST+PVVVDGDL+YFNLL
Sbjct: 181 ARAMKDIGSVLESSCAPILMNVYPYFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300
           DAMVDSFY ALEKIGVEGVRIGISETGWP+KGNEPFTSV+NALTYN+N V+HVSSGVGTP
Sbjct: 241 DAMVDSFYTALEKIGVEGVRIGISETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLY 360
           +MPNLQYD+V F +    L       NF F  P G GT+GVNYGLNGDNLP PSDVINLY
Sbjct: 301 KMPNLQYDIVFFSIPFFLLFLCFNVLNFLF--PGGSGTIGVNYGLNGDNLPIPSDVINLY 360

Query: 361 GRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYY 420
            RCGINI+RLFEPNHGVLDALQGKKDLVLWLGTRNEDI+GFATNQLAAN WVNANVVPYY
Sbjct: 361 RRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYY 420

Query: 421 KNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVVAMTALGVSYP 480
           K+VNI+YIT+GNEVVPGDAAAPFVAN IKNIMQALV++GIKSDIKVTTVVAMT L VSYP
Sbjct: 421 KDVNISYITVGNEVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYP 480

Query: 481 PSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVV 540
           PSAGAFT SAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTS+TPVVV
Sbjct: 481 PSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVV 540

Query: 541 DGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLV 600
           DG+LQYFNLFDAMVDSFYAALEKIDA EIRIGISETGWPT+GNEPFTSV+NALTYNKNLV
Sbjct: 541 DGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGISETGWPTSGNEPFTSVDNALTYNKNLV 600

Query: 601 KHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPNMNPVYPFWNC 654
            HVTSGVGTPKRPNLKYD VLFEMFNEDLKAPGVEQNFGFF PNMNPVYPFWNC
Sbjct: 601 NHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGVEQNFGFFFPNMNPVYPFWNC 652

BLAST of CsGy4G017300 vs. NCBI nr
Match: XP_023521459.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672 bits (1735), Expect = 1.11e-235
Identity = 367/648 (56.64%), Postives = 433/648 (66.82%), Query Frame = 0

Query: 7   TTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHE 66
           + +LL +FIFS Q L +SV GAVGVNYGLN DNLP P+EV+ LY RCGI+I+R+FEPNH 
Sbjct: 8   SAALLLLFIFSDQALLKSVLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHS 67

Query: 67  ILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITVGNEVVP 126
           +L AL G+ NLV+WLGTRNEDI+GFA +Q  AN+WVNANVV YYK VNIAYITVGNEVVP
Sbjct: 68  VLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVP 127

Query: 127 GDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIAARTMKD 186
            DAA+PFVA AIK +MQAL  AGV+SDIK+++                            
Sbjct: 128 DDAAAPFVAKAIKKIMQALVIAGVKSDIKISS---------------------------- 187

Query: 187 IGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLLDAMVDS 246
                                            ALFT+                      
Sbjct: 188 ---------------------------------ALFTT---------------------- 247

Query: 247 FYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTPRMPNLQ 306
                                       P+T+++                    R P+  
Sbjct: 248 ----------------------------PYTTMDFF------------------RPPSAA 307

Query: 307 YDVVLFEMFNEDLKSPGVEQNFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLYGRCGIN 366
             ++LF   ++ L              SG G +GVNYGLNGDNLP PS+V+ LYGRCGI+
Sbjct: 308 L-LLLFIFSDQALLI------------SGLGAVGVNYGLNGDNLPVPSEVVELYGRCGID 367

Query: 367 ILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYYKNVNIA 426
           I+R+FEPNH VLDAL+G+++LV+WLGTRNEDIQGFA +QLAAN+WVNA+VVPYY++VNIA
Sbjct: 368 IVRVFEPNHWVLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNAHVVPYYESVNIA 427

Query: 427 YITIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAF 486
           YIT+GNEVVP DAAAPFVA  IKNIMQALV  G+KSDIKVTTVV MT L VSYPPSAGAF
Sbjct: 428 YITVGNEVVPDDAAAPFVAKAIKNIMQALVIAGVKSDIKVTTVVPMTPLEVSYPPSAGAF 487

Query: 487 TGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVVDGNLQY 546
           + +A G +KDI  VLG+SGAP+L+NVYPYFAYASNPQQISL+YALFTS+TPVVVDGNLQY
Sbjct: 488 SATAIGPLKDITQVLGTSGAPLLLNVYPYFAYASNPQQISLDYALFTSTTPVVVDGNLQY 513

Query: 547 FNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLVKHVTSG 606
           FNLFDAMVDSFY+ALEKI A  +RI ISETGWP+ GN+P TSVENA TYN+N V+HV SG
Sbjct: 548 FNLFDAMVDSFYSALEKIGAEGVRIDISETGWPSKGNDPLTSVENAATYNRNFVEHVRSG 513

Query: 607 VGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPNMNPVYPFWNC 654
            GTP+RPN+KYD VLFEMFNE+LK  GVEQNFG F  NM PVY FWNC
Sbjct: 608 AGTPRRPNVKYDGVLFEMFNENLKTAGVEQNFGLFYSNMLPVYSFWNC 513

BLAST of CsGy4G017300 vs. NCBI nr
Match: XP_031740439.1 (glucan endo-1,3-beta-glucosidase [Cucumis sativus])

HSP 1 Score: 664 bits (1713), Expect = 4.78e-235
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0

Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI
Sbjct: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120
           FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV
Sbjct: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120

Query: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA
Sbjct: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180

Query: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240
           ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL
Sbjct: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300
           DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP
Sbjct: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS 334
           RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS
Sbjct: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS 334

BLAST of CsGy4G017300 vs. ExPASy TrEMBL
Match: A0A5A7V7B6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold548G002240 PE=3 SV=1)

HSP 1 Score: 1186 bits (3068), Expect = 0.0
Identity = 594/664 (89.46%), Postives = 626/664 (94.28%), Query Frame = 0

Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M LFFPTTSLL IFIFSHQ LH SVFGAVGVNYGLNSDNLPKP+EVINLYERCGINIVRI
Sbjct: 1   MALFFPTTSLLLIFIFSHQILHISVFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120
           FEPNHE+L AL  KENLVLWLGTRNEDI+GFATNQEVA+AWVNANVV YYKDVNIAYITV
Sbjct: 61  FEPNHEVLDALRCKENLVLWLGTRNEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITV 120

Query: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGDAA+PFVANAI+N+MQAL NA VQSDIKVTTVVAMT LEVSSPPSAGAF+AIA
Sbjct: 121 GNEVVPGDAATPFVANAIQNIMQALVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIA 180

Query: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240
           AR MKDIG+VLESSCAPIL+NVYPYFAYASNPQQI +SYALFTST+PVVVDGDL+YFNLL
Sbjct: 181 ARAMKDIGSVLESSCAPILMNVYPYFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300
           DAMVDSFY ALEKIGVEGVRIGISETGWP+KGNEPFTSV+NALTYN+N V+HVSSGVGTP
Sbjct: 241 DAMVDSFYTALEKIGVEGVRIGISETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS----------GFGTLGVNYGLNGDNL 360
           +MPNLQYD+VLFEMFNEDLKSPGVEQNFGFF P+          G GT+GVNYGLNGDNL
Sbjct: 301 KMPNLQYDIVLFEMFNEDLKSPGVEQNFGFFYPNMNPVYSFWNCGSGTIGVNYGLNGDNL 360

Query: 361 PTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANA 420
           P PSDVINLY RCGINI+RLFEPNHGVLDALQGKKDLVLWLGTRNEDI+GFATNQLAAN 
Sbjct: 361 PIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANT 420

Query: 421 WVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVV 480
           WVNANVVPYYK+VNI+YIT+GNEVVPGDAAAPFVAN IKNIMQALV++GIKSDIKVTTVV
Sbjct: 421 WVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVV 480

Query: 481 AMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYA 540
           AMT L VSYPPSAGAFT SAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYA
Sbjct: 481 AMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYA 540

Query: 541 LFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVE 600
           LFTS+TPVVVDG+LQYFNLFDAMVDSFYAALEKIDA EIRIGISETGWPT+GNEPFTSV+
Sbjct: 541 LFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGISETGWPTSGNEPFTSVD 600

Query: 601 NALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPNMNPVYP 654
           NALTYNKNLV HVTSGVGTPKRPNLKYD VLFEMFNEDLKAPGVEQNFGFF PNMNPVYP
Sbjct: 601 NALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGVEQNFGFFFPNMNPVYP 660

BLAST of CsGy4G017300 vs. ExPASy TrEMBL
Match: A0A1S4DV56 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV=1)

HSP 1 Score: 1144 bits (2958), Expect = 0.0
Identity = 578/654 (88.38%), Postives = 610/654 (93.27%), Query Frame = 0

Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           M LFFPTTSLL IFIFSHQ LH SVFGAVGVNYGLNSDNLPKP+EVINLYERCGINIVRI
Sbjct: 1   MALFFPTTSLLLIFIFSHQILHISVFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120
           FEPNHE+L AL  KENLVLWLGTRNEDI+GFATNQEVA+AWVNANVV YYKDVNIAYITV
Sbjct: 61  FEPNHEVLDALRCKENLVLWLGTRNEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITV 120

Query: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGDAA+PFVANAI+N+MQAL NA VQSDIKVTTVVAMT LEVSSPPSAGAF+AIA
Sbjct: 121 GNEVVPGDAATPFVANAIQNIMQALVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIA 180

Query: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240
           AR MKDIG+VLESSCAPIL+NVYPYFAYASNPQQI +SYALFTST+PVVVDGDL+YFNLL
Sbjct: 181 ARAMKDIGSVLESSCAPILMNVYPYFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300
           DAMVDSFY ALEKIGVEGVRIGISETGWP+KGNEPFTSV+NALTYN+N V+HVSSGVGTP
Sbjct: 241 DAMVDSFYTALEKIGVEGVRIGISETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLY 360
           +MPNLQYD+V F +    L       NF F  P G GT+GVNYGLNGDNLP PSDVINLY
Sbjct: 301 KMPNLQYDIVFFSIPFFLLFLCFNVLNFLF--PGGSGTIGVNYGLNGDNLPIPSDVINLY 360

Query: 361 GRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYY 420
            RCGINI+RLFEPNHGVLDALQGKKDLVLWLGTRNEDI+GFATNQLAAN WVNANVVPYY
Sbjct: 361 RRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYY 420

Query: 421 KNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVVAMTALGVSYP 480
           K+VNI+YIT+GNEVVPGDAAAPFVAN IKNIMQALV++GIKSDIKVTTVVAMT L VSYP
Sbjct: 421 KDVNISYITVGNEVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYP 480

Query: 481 PSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVV 540
           PSAGAFT SAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTS+TPVVV
Sbjct: 481 PSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVV 540

Query: 541 DGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLV 600
           DG+LQYFNLFDAMVDSFYAALEKIDA EIRIGISETGWPT+GNEPFTSV+NALTYNKNLV
Sbjct: 541 DGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGISETGWPTSGNEPFTSVDNALTYNKNLV 600

Query: 601 KHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPNMNPVYPFWNC 654
            HVTSGVGTPKRPNLKYD VLFEMFNEDLKAPGVEQNFGFF PNMNPVYPFWNC
Sbjct: 601 NHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGVEQNFGFFFPNMNPVYPFWNC 652

BLAST of CsGy4G017300 vs. ExPASy TrEMBL
Match: A0A0A0L1Z1 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377700 PE=3 SV=1)

HSP 1 Score: 664 bits (1713), Expect = 2.32e-235
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0

Query: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60
           MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI
Sbjct: 1   MPLFFPTTSLLFIFIFSHQFLHRSVFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRI 60

Query: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120
           FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV
Sbjct: 61  FEPNHEILHALCGKENLVLWLGTRNEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITV 120

Query: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180
           GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA
Sbjct: 121 GNEVVPGDAASPFVANAIKNMMQALDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIA 180

Query: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240
           ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL
Sbjct: 181 ARTMKDIGNVLESSCAPILVNVYPYFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLL 240

Query: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300
           DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP
Sbjct: 241 DAMVDSFYAALEKIGVEGVRIGISETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTP 300

Query: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS 334
           RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS
Sbjct: 301 RMPNLQYDVVLFEMFNEDLKSPGVEQNFGFFDPS 334

BLAST of CsGy4G017300 vs. ExPASy TrEMBL
Match: A0A0A0L3A5 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377200 PE=3 SV=1)

HSP 1 Score: 649 bits (1673), Expect = 2.76e-229
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 334 SGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGT 393
           SGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGT
Sbjct: 24  SGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGT 83

Query: 394 RNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQ 453
           RNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQ
Sbjct: 84  RNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQ 143

Query: 454 ALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVY 513
           ALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVY
Sbjct: 144 ALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVY 203

Query: 514 PYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGI 573
           PYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGI
Sbjct: 204 PYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGI 263

Query: 574 SETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPG 633
           SETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPG
Sbjct: 264 SETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPG 323

Query: 634 VEQNFGFFSPNMNPVYPFWNC 654
           VEQNFGFFSPNMNPVYPFWNC
Sbjct: 324 VEQNFGFFSPNMNPVYPFWNC 344

BLAST of CsGy4G017300 vs. ExPASy TrEMBL
Match: A0A5D3D518 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001700 PE=3 SV=1)

HSP 1 Score: 605 bits (1560), Expect = 3.94e-212
Identity = 297/321 (92.52%), Postives = 309/321 (96.26%), Query Frame = 0

Query: 334 SGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGT 393
           SG GT+GVNYGLNGDNLP PSDVINLY RCGINI+RLFEPNHGVLDALQGKKDLVLWLGT
Sbjct: 24  SGSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGT 83

Query: 394 RNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQ 453
           RNEDI+GFATNQLAAN WVNANVVPYYK+VNI+YIT+GNEVVPGDAAAPFVAN IKNIMQ
Sbjct: 84  RNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQ 143

Query: 454 ALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVY 513
           ALV++GIKSDIKVTTVVAMT L VSYPPSAGAFT SAAGIMKDIANVLGSSGAPILVNVY
Sbjct: 144 ALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVY 203

Query: 514 PYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGI 573
           PYFAYASNPQQISLNYALFTS+TPVVVDG+LQYFNLFDAMVDSFYAALEKIDA EIRIGI
Sbjct: 204 PYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGI 263

Query: 574 SETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPG 633
           SETGWPT+GNEPFTSV+NALTYNKNLV HVTSGVGTPKRPNLKYD VLFEMFNEDLKAPG
Sbjct: 264 SETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPG 323

Query: 634 VEQNFGFFSPNMNPVYPFWNC 654
           VEQNFGFF PNMNPVYPFWNC
Sbjct: 324 VEQNFGFFFPNMNPVYPFWNC 344

BLAST of CsGy4G017300 vs. TAIR 10
Match: AT1G77780.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 1.5e-84
Identity = 156/314 (49.68%), Postives = 214/314 (68.15%), Query Frame = 0

Query: 340 GVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQ 399
           GV YG NGDNLP+P+  ++LY +  +  +RL+EP   ++ +LQG   L++ +G RNE I+
Sbjct: 25  GVCYGRNGDNLPSPAKTVSLYKKINVGGIRLYEPFPDLIVSLQG-TGLLVAIGPRNEAIK 84

Query: 400 GFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQALVDVG 459
             A     A  W    + P YKNV   +IT+GNEV+ G+    +V   +KNI  AL ++G
Sbjct: 85  TLAEEYQFALNWDKTFIAP-YKNVAFNWITVGNEVIEGEIGR-YVPQAMKNIKAALTEIG 144

Query: 460 IKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYA 519
             S I VTTV++  AL  SYPPSAG F  +   ++ +I ++L S+ +P++VNVYPYFAYA
Sbjct: 145 -NSKIHVTTVISTAALANSYPPSAGVFKPAITELITEIVSILSSTDSPLMVNVYPYFAYA 204

Query: 520 SNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISETGWP 579
           S+P  +SL YA F S++PVV DG  QY N+FDA +D+F  ALEKI+ G +++ ++ETGWP
Sbjct: 205 SDPSHVSLEYATFRSTSPVVTDGKYQYTNIFDATLDAFNVALEKINHGSVKVYVAETGWP 264

Query: 580 TNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFG 639
           T GN+P+TSVENA  YN+ L+K +T+G GTP+RPN+      FEMFNEDLK   VEQ+FG
Sbjct: 265 TRGNDPYTSVENARAYNQGLLKKLTTGKGTPRRPNVPVITFFFEMFNEDLKQGAVEQSFG 324

Query: 640 FFSPNMNPVYPFWN 654
           FF PNM PVY  WN
Sbjct: 325 FFDPNMAPVYDMWN 334

BLAST of CsGy4G017300 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 289.3 bits (739), Expect = 7.9e-78
Identity = 152/324 (46.91%), Postives = 214/324 (66.05%), Query Frame = 0

Query: 334 SGFGT----LGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVL 393
           SGF T    +G+NYGL GDNLP+PS+VINLY   GI+ +R+F+PN  VL+AL+G +D+ +
Sbjct: 25  SGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEV 84

Query: 394 WLGTRNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIK 453
            +G +++D+   A ++ A   W  AN+  Y  +VNI +IT+GNEV+PG      +   + 
Sbjct: 85  TVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGP-----IGPQVL 144

Query: 454 NIMQALVDVGIKS---DIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGA 513
            +MQ+L ++ +KS    I ++TVVAM+ L  SYPPSAG FT  A   +  +  +L  +  
Sbjct: 145 PVMQSLTNL-VKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTST 204

Query: 514 PILVNVYPYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDA 573
           PILVN+YPYFAYAS+P  I L+YA F + + VV DG+L Y N+FDA+ D+F  A+EK   
Sbjct: 205 PILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGV 264

Query: 574 GEIRIGISETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFN 633
             + + +SETGWP+ GN  FT+   A TYN+N VKH+ SG GTPKRPN   +  LF  FN
Sbjct: 265 KNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFN 324

Query: 634 EDLKAPGVEQNFGFFSP-NMNPVY 650
           E+ K  G EQNFG ++P +M P+Y
Sbjct: 325 ENQKPAGTEQNFGLYNPSDMKPIY 342

BLAST of CsGy4G017300 vs. TAIR 10
Match: AT1G77790.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.3e-77
Identity = 154/309 (49.84%), Postives = 206/309 (66.67%), Query Frame = 0

Query: 343 YGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFA 402
           YG NG+NLPTP+D + LY    I+ +R++EP   +L+AL+G   L++  G RNEDIQ  A
Sbjct: 34  YGRNGNNLPTPADTVALYKSNNIDAIRMYEPFADMLEALRG-SGLLVAFGPRNEDIQSLA 93

Query: 403 TNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKS 462
            +  AA  +V+  + PY  +V I +ITIGNEV PG+  A FVA  IKN+  AL + G+ +
Sbjct: 94  HDPAAATNFVSTWITPYQNDVAIKWITIGNEVFPGE-IAQFVAAAIKNVNVALTNSGV-T 153

Query: 463 DIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNP 522
            I VTTV+AMTAL  +YPPSA  F      IM +I ++L  + +P++ N+YPYFAYAS+P
Sbjct: 154 GISVTTVLAMTALTNTYPPSAATFLPDLTEIMTEITSILSETNSPLMTNIYPYFAYASDP 213

Query: 523 QQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNG 582
             ISL+YA F S+TPVV+DG+L Y N+F+AMVD F AALEKI+A  + + ++ETGWPT G
Sbjct: 214 YHISLDYASFKSNTPVVIDGDLYYNNMFEAMVDGFNAALEKINAANVVVMVAETGWPTEG 273

Query: 583 NEPFTSVENALTYNKNL--VKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGF 642
           N P TSV+NA  YN  +           TP+R N   DV LF MF E+ K   VEQ+FG 
Sbjct: 274 NPPHTSVDNAKAYNMGIRTCGRSAERKRTPRRQNTPVDVFLFAMFKENQKDGPVEQSFGI 333

Query: 643 FSPNMNPVY 650
           F+P+M PVY
Sbjct: 334 FAPDMTPVY 339

BLAST of CsGy4G017300 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 285.4 bits (729), Expect = 1.1e-76
Identity = 151/345 (43.77%), Postives = 218/345 (63.19%), Query Frame = 0

Query: 309 VVLFEMFNEDLKSPGVEQNFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINIL 368
           VV+    N D  +     + GF + +   ++G+NYGL GDNLP+PS VI LY    I  +
Sbjct: 17  VVIVNYNNSDFVN--AANSIGFVNAA--NSIGLNYGLLGDNLPSPSKVITLYKSIDITKI 76

Query: 369 RLFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYI 428
           R+F+PN  VL+AL+G +D+ + +G R++D+   + ++ A   W   N+ PY  ++NIA+I
Sbjct: 77  RIFDPNTEVLNALRGHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIAFI 136

Query: 429 TIGNEVVPGDAAAPFVANGIKNIMQALVDVGIKS---DIKVTTVVAMTALGVSYPPSAGA 488
           T+GNEV+PG      +   +  +MQ+L ++ +KS    I ++TVVAM  L  SYPPSAG 
Sbjct: 137 TVGNEVIPGP-----IGPQVLPVMQSLTNL-VKSRNLPISISTVVAMWNLEQSYPPSAGM 196

Query: 489 FTGSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVVDGNLQ 548
           FT  A   +  +  +L  + +PILV +YPYF+YAS+P  I L+YA F +   VV DG+L 
Sbjct: 197 FTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGSLG 256

Query: 549 YFNLFDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLVKHVTS 608
           Y N+FDA+ D+F  A+EK    ++ + +SETGWP+ GN   T+ + A TYN+N VKH+ S
Sbjct: 257 YSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIAS 316

Query: 609 GVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPN-MNPVY 650
           G GTPKRPN   D  LF  FNE+ K  G EQNFG ++PN M P+Y
Sbjct: 317 GKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFGLYNPNDMKPIY 351

BLAST of CsGy4G017300 vs. TAIR 10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 268.1 bits (684), Expect = 1.9e-71
Identity = 139/327 (42.51%), Postives = 205/327 (62.69%), Query Frame = 0

Query: 327 NFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKD 386
           N GF D +    +G+NYGL GDNLP PS+VINLY    +  +R+F+    VL+A +G ++
Sbjct: 23  NVGFVDAA--TNIGLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTTTDVLNAFRGNRN 82

Query: 387 LVLWLGTRNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVAN 446
           + + +  +N+D++  + ++ A N W   N+ PY  +VNI +I +GNEV+PG+     + +
Sbjct: 83  IGVMVDVKNQDLEALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEVIPGE-----IGS 142

Query: 447 GIKNIMQALVDVGIKS---DIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGS 506
            +  +M++L ++ +KS    I ++T VAMT LG SYPPSAG F   A   +  +   L  
Sbjct: 143 YVLPVMKSLTNI-VKSRSLPILISTTVAMTNLGQSYPPSAGDFMPQAREQLTPVLKFLSQ 202

Query: 507 SGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEK 566
           +  PILVN+YPYFAYA++P  I L+YA+F ++  VV DG L Y N+FD + D+F  A+EK
Sbjct: 203 TNTPILVNIYPYFAYAADPINIQLDYAIFNTNKVVVQDGPLGYTNMFDVIFDAFVWAMEK 262

Query: 567 IDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFE 626
               ++ + ++ETGWP+ GN   T+ + A  YN N VKHV SG GTPKRP       LF 
Sbjct: 263 EGVKDLPMVVTETGWPSAGNGNLTTPDIASIYNTNFVKHVESGKGTPKRPKSGISGFLFA 322

Query: 627 MFNEDLKAPGVEQNFGFFSP-NMNPVY 650
            FNE+ K  G EQNFG ++P +M P+Y
Sbjct: 323 TFNENQKPAGTEQNFGLYNPTDMKPIY 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O223172.0e-8147.95Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2[more]
O493521.1e-7646.91Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN... [more]
O493531.6e-7543.77Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q024394.2e-6845.28Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4... [more]
Q014131.6e-6744.90Glucan endo-1,3-beta-glucosidase B OS=Solanum lycopersicum OX=4081 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAE8649635.10.0100.00hypothetical protein Csa_012736 [Cucumis sativus][more]
KAA0064262.10.089.46putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa][more]
XP_016899853.10.088.38PREDICTED: probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo][more]
XP_023521459.11.11e-23556.64probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita pepo subsp. pepo][more]
XP_031740439.14.78e-235100.00glucan endo-1,3-beta-glucosidase [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A5A7V7B60.089.46(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S4DV560.088.38(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV... [more]
A0A0A0L1Z12.32e-235100.00(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377700 PE=3... [more]
A0A0A0L3A52.76e-229100.00(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377200 PE=3... [more]
A0A5D3D5183.94e-21292.52(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT1G77780.11.5e-8449.68Glycosyl hydrolase superfamily protein [more]
AT5G20330.17.9e-7846.91beta-1,3-glucanase 4 [more]
AT1G77790.11.3e-7749.84Glycosyl hydrolase superfamily protein [more]
AT5G20340.11.1e-7643.77beta-1,3-glucanase 5 [more]
AT5G20390.11.9e-7142.51Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 338..653
e-value: 2.7E-112
score: 376.9
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 29..336
e-value: 9.5E-109
score: 365.1
NoneNo IPR availablePANTHERPTHR32227:SF248GLYCOSYL HYDROLASE SUPERFAMILY PROTEINcoord: 337..651
coord: 21..333
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 29..334
e-value: 1.6E-86
score: 290.8
coord: 339..649
e-value: 2.5E-89
score: 300.0
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 337..651
coord: 21..333
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 29..334
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 339..651

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G017300.2CsGy4G017300.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds