CsGy4G015200 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G015200
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionVacuolar cation/proton exchanger
LocationGy14Chr4: 19895367 .. 19900307 (-)
RNA-Seq ExpressionCsGy4G015200
SyntenyCsGy4G015200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCATTCTATAAAAATGTTTATTCCTACCGCCATATCTCTCAAAGAAAACATCTGAAACCAACACAGCTTTTGCCAACTTCCCCAACTAGTTCTTCTCTGATTACCACACCTGTTTGGATACCCCTATTTCAACCAAAAACACAAAACCATGGCTTCCCAAACAGAGCCTTGGCTAATGGAAAATGGAACCCTTAAGCGCCTAACCAAAGAGCATCGACATGGCCATGGTCGAACAGCTCATAACATGTCCTCCTCTTCCTTACGCAAAAAGTCCGATTTAACCCTCGTCTCCAAAATCCAATTCGGCTGTCTCCGTAAGTTTTGTATTAATCTTCAGGAGGTCATTTTGGGAACCAAACTTTCCATTCTCTTCCCCGCGATTCCTCTCGCTATTGTCGCTCAGAATTTTGGCTTTGGAAGAGTGAGAATCCTCCATAATCTTCGTGAAACTACATGTCGTTTCTTTATTAATAATTGTTTTTTTGGACTAATAAATAATAATAATTTGTTTTGTAGCCTTGGATTTTTGCATTGAGCTTGCTGGGCCTTACGCCATTGGCTGAACGTGTCAGCTTCCTAACCGAGTAAGCTTTTCTAGCTTTTCATTAGTGTATTTTTAATTTTGGTAATCAATATATCAAATTATTATTATATTCCGAAACCTTTTCCAATATTATACTAACCTTTCTCTTCTTCTTCTTGTTTTGATTCGAACTTTGAAGGCAAATCGCTTATTTCACTGGTCCAACAGGTAACAATTCGCTTATGTTTTATTTCATTTGTAACCCATTCATATATATATATATATAACCGTTGGAAATCGGTAGAAAATTTAAATATGCAAATTGTTTATGTTTTCCAGGAAATGCTCCTGAAATGAAAAAAAAAATGTGGCTAAGCTATAGCTTAATTAATCAAATATCAATTATCATAATGTTTTTTATGCTTTGGTTTCCCACCATCTATAGTTATTGATTTTTTTTAATAGATATGGTCATTCTAAGCTAATTATTGATTCAATAAACACAACTTTATTATTAATTCAATTTCAACCATTTGGACTACACCTATTTTTACAAAAGAAAAAAAAATTGAAATATACGTACATGTATTTCTGCATAACGAAATTTTTTTGAGGTACAATAATGTAATTTTTATTTATATATGACGAGGAAGTTTTTAGGTACAACAATGTAATTTTTATTTATATTATTTGTTTTCTTATCCATTTCTCATCCACAAGTACACAATAGCTATAATGACGAATGCATGTATGGCTTTTGCTACTCAAGGGAAAAGCCAACCGTACAAAAACAGTTTCCATTTGTTTTTATTTTTTAAAAAAATGAAAAAGATAATGAACTTCTATAATTTTGAAAACCCTAAAATGATAGTGACATACATAAGGGAGGCCCGTGAAGGAGAATATTTAGATTGGTAAGGTTATTAATTAAGTTTGATAACGATATATACTTTTTTTTTTTTTTTCTGTTTCTTGGGTTCTAAGAACTAGAAACAAGTAATATGTTTTATATCAAATCAGTTTTCAAAATTCAGTCTAAATTTTAAAAAAATTTCTCATATTTTAGAAATAAGAAATGAGATGTATTATCAAAATAAGTATGTTCTTCCAATAAAAATAGGAAATGATCGAATGTACTTCAAATTAATGAATGAAATTTAAATGTGTATTGTATATGTATATGTAGTTGGGGGGCTTTTGAATGCAACATGTGGAAATGCAACGGAGCTGATAATTGCAATATTAGCACTGACACAAAGGAAGATAGACGTGGTGAAGTATTCTCTATTGGGTTCTGTTCTTTCAAACCTTCTTCTTGTTCTTGGAACTTCTCTCTTTTGTGGTGGAATTGCTAATATTAGACGGGAACAAAACTATGACAGAGTAATTACCTCTCTCTCTGATCTCTCTCATTTGTTCTATTGTTTTTAGTATTTTCATTCAAAGATCGAGTTAAAACTAGATTCGGATTTTTAAAATATAAAAACTACTCTTGAAATGTGTTAGAAGTGATTTTGAAGTACTTAGATAAAAATGATTTTAATAAATTCAAAATATTTTCAAATACATATTTAAATTTGAGCAATACACAGAAATTTCTGATTTTATTAACCCTGTAATTAAATAAGCAATCTTTTTAACAGAGGCAAGCTGATGTCAACTCCCTCATGTTGATGCTATCTTTACTGTGCCACCTATTGCCTTTACTCTTTGGCTACGCTGCCGCCTCTGCCTCCACGGCCGCCGCCTCGACCCTCCATCTGTCCAGAGCTAGCAGCATTGTAATGCTCACTGCTTACATTGCTTACTTGGTCTTTCAACTCTGGACTCATCGACAACTGTTTGAAGCTGAGGTACAATGATTGTTCCATTGAATATTATTTTATGTTTAATAGATCTAATGCATGTTAAACTTATTTCAGCTACGTATGTTACAAAGTTTTGTTCGATGATTACAGGATTTTTTAAAATTCAAGTTCACAATCGAATTGGTTTCTTTTAGTAATTGATGATATAGCAAGTGATGTTGATCGGAATTAACAAACTCTACTATTTGCCAAGAACTATGGTTGTGAATCAAGCATTTGGCAATATGTTTCTAGATATAACCAACCTAAGGTGGATGATTTTGATTGTTTGACAACAAATCAACCACACCATACCAAAATTTTGATAAAAAAGATTAAAAAGATCACTTTAAATATTTTATTTTATCAATAAATTATAAAAATAATTTAATCTTCTACAAAATTTGAAAATATATTCAATTCTTTAAATGTAAATTATAATTATTATATAAGTTCACAAAGATAAAAATTAACTGTAGTATTGGAATATTAATCAGGAAGGTGAAGACGATGACGTTCCAGGAGAAGAGGCAGTGATCGGATTCTGGAGTGCATTTGCATGGCTTGTTGGAATGACACTCGTTATTGCTTTGCTTTCCGAGTACGTTGTCAACACAATTGAGGTAAAATTACTAGAAAAATACCCTTTTCACTCTTGATTTGATTTGACCCGGGATAAGTTTTCATTTTATTATTTAACTTTTGAAATGTTATATTTTTAATTCTGATTTTTCAGGAATGCTTACTTTGAGTTGGTTGTGTTATTTTGTAGGAAGCATCTACTACTTGGGGTTTGTCTGTGAGTTTCCTTAGCATAATCTTGCTCCCTATTGTTGGAAATGCCGCCGAACACGCGGGGGCTATCATATTTGCCTTCAAGAATAAGTTGGTAAGCTCTAAAAAATTCACAAATTTACTACAATCAAAATTAATTTTAATTTGCAGATTAAGGGAGGATTACTGTTATATTATATCTAATCATTGTATACTTAATTTTAATTTGTTTTGGTTTTCAGGACATATCTTTGGGGGTTGCCCTGGGATCTGCAACACAAATTGCACTATTTGTGGTAAGTATATGTATGTGTGATTAGTGAAAACTAGTTCAAAGTTTCAAACCTCCCCTAGGAAATTGAAGTGTGGAAATATATATTTGGAAACTGACCCCTGAAGAAAATGTAACATGCAGATTCCAATGTGTGTGATAGTAGCTTGGACAATGGGAGTGGACATGGACCTAAATTTCAACCTAATGGAAACAGGGTCACTTGGATTAGCAATTATTGCCACAGCCTTCACTTTACAAGATGGAACCTCTCATTATATTAAAGGCCTTGTTCTTCTTCTTTGCTACATTATCATTGCTGCTTGCTTCTTTGTTTCCAAGACACAAGAAGGTGATTTTCCAAAATAATTTTCCCATCTTTTCTAGGGGCAGTTTAAATTAATTTAAATAGTGATTTCGATAGGAAAATGATATTGAGACTACGGAAATTTAATAATAACTAAAATAATTATTAGTTTAAAATTTTGTACCTTTCAGAATTAATTTGTTTTTAGCTAATTAATTAATTTGTTAAGAGCTAATTAAACAACACTCTAAATTCACTTCTCTCTTTGTGTTTCCCCCCACAGATAATAACAATGCTCCCAACTTGGGACTCCAATCTTCAAACCAAGCAATCTTCAGAGCTTAAAGGTAAATAATAATAATAATAATAATTAGATTACCCTTTTAATTAATTTAAATAAACACTTTTTAACTTCCATTTAAATTTGACTGATAACTTTATATATTATGATTAATTGGATGCAGGTTTGAAAAATATTCTCAAGTCAAAATCAAATTCGAAAAGAGAGGGACTTCTTTGAAATCTCATTTGTAAAGAAGGACCAAAAAAAAAAAAAACCAAATCAAATGGAATTTTCATTAATTCTTTGATTTTTCATGGCATCAATCGGTTCGATCCCTGCCTGCTGGAAAGCTTAGAAAATTTGAAGATGTTGGGAGTTTAATGAAAGCAGTTTGTGGGCTGAAAATTAAAGCATTCAAATCTGCGGGTTTATTATATGCAATATTTGTATGCTTTTTATATGAATTATTTTCTGTTTTTCTGTTTTTAATTTTATTGTTCGTCTTCTTTTGCTTTAATTGTTGTAATGTAATAATTATTTCTCAAGTTTAAGTAATTAATGAAATAATATGTCCTCAATTCTGTTTTCTTGTGAAATTTTTAGTCTTGGATTCCATTGCCATTTCTTTATAAAAAAAAAATTATGTTACTTTTATGGGATAGCTTGGACCAAAAAAAGGACCCTAAGAATATTGATAGTAACAAGGATAGTTGCAACTATATTATAAAATTTAAGTATATAATAATAATATTTATATATTTTTTTAAAACTTTGGAATACGCTTAATTATTATATTTAAAAATAACTAAATGGTTAATTGTAAATTTAGCAATTAAATTCAAATTAATTAAATATACAGC

mRNA sequence

CTTTCATTCTATAAAAATGTTTATTCCTACCGCCATATCTCTCAAAGAAAACATCTGAAACCAACACAGCTTTTGCCAACTTCCCCAACTAGTTCTTCTCTGATTACCACACCTGTTTGGATACCCCTATTTCAACCAAAAACACAAAACCATGGCTTCCCAAACAGAGCCTTGGCTAATGGAAAATGGAACCCTTAAGCGCCTAACCAAAGAGCATCGACATGGCCATGGTCGAACAGCTCATAACATGTCCTCCTCTTCCTTACGCAAAAAGTCCGATTTAACCCTCGTCTCCAAAATCCAATTCGGCTGTCTCCGTAAGTTTTGTATTAATCTTCAGGAGGTCATTTTGGGAACCAAACTTTCCATTCTCTTCCCCGCGATTCCTCTCGCTATTGTCGCTCAGAATTTTGGCTTTGGAAGACCTTGGATTTTTGCATTGAGCTTGCTGGGCCTTACGCCATTGGCTGAACGTGTCAGCTTCCTAACCGAGCAAATCGCTTATTTCACTGGTCCAACAGTTGGGGGGCTTTTGAATGCAACATGTGGAAATGCAACGGAGCTGATAATTGCAATATTAGCACTGACACAAAGGAAGATAGACGTGGTGAAGTATTCTCTATTGGGTTCTGTTCTTTCAAACCTTCTTCTTGTTCTTGGAACTTCTCTCTTTTGTGGTGGAATTGCTAATATTAGACGGGAACAAAACTATGACAGAAGGCAAGCTGATGTCAACTCCCTCATGTTGATGCTATCTTTACTGTGCCACCTATTGCCTTTACTCTTTGGCTACGCTGCCGCCTCTGCCTCCACGGCCGCCGCCTCGACCCTCCATCTGTCCAGAGCTAGCAGCATTGTAATGCTCACTGCTTACATTGCTTACTTGGTCTTTCAACTCTGGACTCATCGACAACTGTTTGAAGCTGAGGAAGGTGAAGACGATGACGTTCCAGGAGAAGAGGCAGTGATCGGATTCTGGAGTGCATTTGCATGGCTTGTTGGAATGACACTCGTTATTGCTTTGCTTTCCGAGTACGTTGTCAACACAATTGAGGAAGCATCTACTACTTGGGGTTTGTCTGTGAGTTTCCTTAGCATAATCTTGCTCCCTATTGTTGGAAATGCCGCCGAACACGCGGGGGCTATCATATTTGCCTTCAAGAATAAGTTGGACATATCTTTGGGGGTTGCCCTGGGATCTGCAACACAAATTGCACTATTTGTGATTCCAATGTGTGTGATAGTAGCTTGGACAATGGGAGTGGACATGGACCTAAATTTCAACCTAATGGAAACAGGGTCACTTGGATTAGCAATTATTGCCACAGCCTTCACTTTACAAGATGGAACCTCTCATTATATTAAAGGCCTTGTTCTTCTTCTTTGCTACATTATCATTGCTGCTTGCTTCTTTGTTTCCAAGACACAAGAAGATAATAACAATGCTCCCAACTTGGGACTCCAATCTTCAAACCAAGCAATCTTCAGAGCTTAAAGGTTTGAAAAATATTCTCAAGTCAAAATCAAATTCGAAAAGAGAGGGACTTCTTTGAAATCTCATTTGTAAAGAAGGACCAAAAAAAAAAAAAACCAAATCAAATGGAATTTTCATTAATTCTTTGATTTTTCATGGCATCAATCGGTTCGATCCCTGCCTGCTGGAAAGCTTAGAAAATTTGAAGATGTTGGGAGTTTAATGAAAGCAGTTTGTGGGCTGAAAATTAAAGCATTCAAATCTGCGGGTTTATTATATGCAATATTTGTATGCTTTTTATATGAATTATTTTCTGTTTTTCTGTTTTTAATTTTATTGTTCGTCTTCTTTTGCTTTAATTGTTGTAATGTAATAATTATTTCTCAAGTTTAAGTAATTAATGAAATAATATGTCCTCAATTCTGTTTTCTTGTGAAATTTTTAGTCTTGGATTCCATTGCCATTTCTTTATAAAAAAAAAATTATGTTACTTTTATGGGATAGCTTGGACCAAAAAAAGGACCCTAAGAATATTGATAGTAACAAGGATAGTTGCAACTATATTATAAAATTTAAGTATATAATAATAATATTTATATATTTTTTTAAAACTTTGGAATACGCTTAATTATTATATTTAAAAATAACTAAATGGTTAATTGTAAATTTAGCAATTAAATTCAAATTAATTAAATATACAGC

Coding sequence (CDS)

ATGGCTTCCCAAACAGAGCCTTGGCTAATGGAAAATGGAACCCTTAAGCGCCTAACCAAAGAGCATCGACATGGCCATGGTCGAACAGCTCATAACATGTCCTCCTCTTCCTTACGCAAAAAGTCCGATTTAACCCTCGTCTCCAAAATCCAATTCGGCTGTCTCCGTAAGTTTTGTATTAATCTTCAGGAGGTCATTTTGGGAACCAAACTTTCCATTCTCTTCCCCGCGATTCCTCTCGCTATTGTCGCTCAGAATTTTGGCTTTGGAAGACCTTGGATTTTTGCATTGAGCTTGCTGGGCCTTACGCCATTGGCTGAACGTGTCAGCTTCCTAACCGAGCAAATCGCTTATTTCACTGGTCCAACAGTTGGGGGGCTTTTGAATGCAACATGTGGAAATGCAACGGAGCTGATAATTGCAATATTAGCACTGACACAAAGGAAGATAGACGTGGTGAAGTATTCTCTATTGGGTTCTGTTCTTTCAAACCTTCTTCTTGTTCTTGGAACTTCTCTCTTTTGTGGTGGAATTGCTAATATTAGACGGGAACAAAACTATGACAGAAGGCAAGCTGATGTCAACTCCCTCATGTTGATGCTATCTTTACTGTGCCACCTATTGCCTTTACTCTTTGGCTACGCTGCCGCCTCTGCCTCCACGGCCGCCGCCTCGACCCTCCATCTGTCCAGAGCTAGCAGCATTGTAATGCTCACTGCTTACATTGCTTACTTGGTCTTTCAACTCTGGACTCATCGACAACTGTTTGAAGCTGAGGAAGGTGAAGACGATGACGTTCCAGGAGAAGAGGCAGTGATCGGATTCTGGAGTGCATTTGCATGGCTTGTTGGAATGACACTCGTTATTGCTTTGCTTTCCGAGTACGTTGTCAACACAATTGAGGAAGCATCTACTACTTGGGGTTTGTCTGTGAGTTTCCTTAGCATAATCTTGCTCCCTATTGTTGGAAATGCCGCCGAACACGCGGGGGCTATCATATTTGCCTTCAAGAATAAGTTGGACATATCTTTGGGGGTTGCCCTGGGATCTGCAACACAAATTGCACTATTTGTGATTCCAATGTGTGTGATAGTAGCTTGGACAATGGGAGTGGACATGGACCTAAATTTCAACCTAATGGAAACAGGGTCACTTGGATTAGCAATTATTGCCACAGCCTTCACTTTACAAGATGGAACCTCTCATTATATTAAAGGCCTTGTTCTTCTTCTTTGCTACATTATCATTGCTGCTTGCTTCTTTGTTTCCAAGACACAAGAAGATAATAACAATGCTCCCAACTTGGGACTCCAATCTTCAAACCAAGCAATCTTCAGAGCTTAA

Protein sequence

MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCINLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIANIRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACFFVSKTQEDNNNAPNLGLQSSNQAIFRA*
Homology
BLAST of CsGy4G015200 vs. ExPASy Swiss-Prot
Match: Q93Z81 (Vacuolar cation/proton exchanger 3 OS=Arabidopsis thaliana OX=3702 GN=CAX3 PE=1 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 4.2e-168
Identity = 327/455 (71.87%), Postives = 374/455 (82.20%), Query Frame = 0

Query: 1   MASQTEPW--LMENG----TLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGC 60
           M S  EPW  + ENG    T K  ++E R  HGRTAHNMSSSSLRKKSDL LV K+    
Sbjct: 1   MGSIVEPWAAIAENGNANVTAKGSSRELR--HGRTAHNMSSSSLRKKSDLRLVQKVPCKT 60

Query: 61  LRKFCINLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTE 120
           L+    NLQEVILGTKL++LF AIPLAI+A ++ +GRP IF LSL+GLTPLAERVSFLTE
Sbjct: 61  LKNILSNLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTE 120

Query: 121 QIAYFTGPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLF 180
           Q+A++TGPTVGGLLNATCGNATELIIAILAL   K+ VVKYSLLGS+LSNLLLVLGTSLF
Sbjct: 121 QLAFYTGPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLF 180

Query: 181 CGGIANIRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAA---ASASTAAASTLHLSR 240
            GGIANIRREQ +DR+QADVN  +L++ LLCHLLPLL  YAA    S S     +L LSR
Sbjct: 181 FGGIANIRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSR 240

Query: 241 ASSIVMLTAYIAYLVFQLWTHRQLFEAEEGE----DDDVPGEEA-VIGFWSAFAWLVGMT 300
            SSIVML AYIAYL+FQLWTHRQLFEA++ +    DD+V  EE  VIGFWS FAWLVGMT
Sbjct: 241 TSSIVMLIAYIAYLIFQLWTHRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMT 300

Query: 301 LVIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360
           +VIALLSEYVV+TIE+AS +WGLSVSF+SIILLPIVGNAAEHAGAIIFAFKNKLDISLGV
Sbjct: 301 IVIALLSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360

Query: 361 ALGSATQIALFVIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKG 420
           ALGSATQI+LFV+P+ VIVAW +G+ MDLNFN++ET SL LAII TAFTLQDGTSHY+KG
Sbjct: 361 ALGSATQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKG 420

Query: 421 LVLLLCYIIIAACFFVSKTQEDNNNAPNLGLQSSN 442
           LVLLLCY+IIAACFFV +  + N+   ++GLQ  N
Sbjct: 421 LVLLLCYVIIAACFFVDQIPQPND--LDVGLQPMN 451

BLAST of CsGy4G015200 vs. ExPASy Swiss-Prot
Match: Q39253 (Vacuolar cation/proton exchanger 1 OS=Arabidopsis thaliana OX=3702 GN=CAX1 PE=1 SV=3)

HSP 1 Score: 587.8 bits (1514), Expect = 1.0e-166
Identity = 321/451 (71.18%), Postives = 365/451 (80.93%), Query Frame = 0

Query: 5   TEPW-LMENG----TLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFC 64
           TEPW + ENG    T K  ++E R   GRTAHNMSSSSLRKKSDL ++ K+ +  L+ F 
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIA 184
           TGPT+GGLLNATCGNATELIIAILALT  K+ VVKYSLLGS+LSNLLLVLGTSLFCGGIA
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 NIRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGY---AAASASTAAASTLHLSRASSIV 244
           NIRREQ +DR+QADVN  +L+L  LCHLLPLL GY     ASA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLTAYIAYLVFQLWTHRQLFEAEEGE---DDDVPGEEAVIGFWSAFAWLVGMTLVIALLS 304
           ML +YIAYLVFQLWTHRQLF+A+E E   DDDV  E AVI FWS FAWLVGMTLVIALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 364
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKLDISLGVALGSATQ
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQ 365

Query: 365 IALFVIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCY 424
           I LFV+P+ +IVAW +G++MDLNF  +ETG L ++II TAFTLQDG+SHY+KGLVLLLCY
Sbjct: 366 IGLFVVPLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCY 425

Query: 425 IIIAACFFVSKTQEDNNNAPNLGLQSSNQAI 445
            IIA CFFV K  +   NA +LG Q+ N  +
Sbjct: 426 FIIAICFFVDKLPQ-KQNAIHLGHQAMNNVV 453

BLAST of CsGy4G015200 vs. ExPASy Swiss-Prot
Match: Q769E5 (Vacuolar cation/proton exchanger 1a OS=Oryza sativa subsp. japonica OX=39947 GN=CAX1a PE=1 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 7.5e-149
Identity = 286/430 (66.51%), Postives = 339/430 (78.84%), Query Frame = 0

Query: 10  MENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCINLQEVILGT 69
           ME G  ++L   H HG  RTAHNMSSSSLRKKSD  LV K+    LR    NLQEV L T
Sbjct: 7   MEAG--RKLAARHPHGRSRTAHNMSSSSLRKKSDAALVRKVPVAPLRPLLANLQEVFLAT 66

Query: 70  KLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPTVGGLLN 129
           KL++LFPA+PLAI AQ F F + W+FALSLLGL PLAERVSFLTEQIA +TGPTVGGLLN
Sbjct: 67  KLAVLFPAVPLAIAAQCFRFDQVWVFALSLLGLIPLAERVSFLTEQIALYTGPTVGGLLN 126

Query: 130 ATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIANIRREQNYDR 189
           ATCGNATELIIA+ AL + KI+VVK SLLGSVLSNLLLVLGTSLFCGG+ N+   Q YDR
Sbjct: 127 ATCGNATELIIALFALLKGKIEVVKCSLLGSVLSNLLLVLGTSLFCGGVVNLGARQPYDR 186

Query: 190 RQADVNSLMLMLSLLCHLLPLLFGYAAAS-----ASTAAASTLHLSRASSIVMLTAYIAY 249
            Q+DV++ +L L++LCH  PLL  YA A+     ++T+AA++L LSRA S VML +Y+AY
Sbjct: 187 NQSDVSTALLFLAVLCHSAPLLLRYAVAAGEHSVSATSAAASLDLSRACSFVMLASYVAY 246

Query: 250 LVFQLWTHRQLFEAEE--GEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTIEE 309
           L FQL THRQLFE +E  G D     EE  +GF SA  WL  MT VI++LSEYVV TIE 
Sbjct: 247 LFFQLKTHRQLFEPQEVDGGDAGDDDEEPALGFASALFWLALMTAVISVLSEYVVGTIEP 306

Query: 310 ASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIPMC 369
            S +WGLSVSF+SIILLPIVGNAAEHAGAIIFA KNKLDI+LGVALGSATQI++FV+P+ 
Sbjct: 307 TSQSWGLSVSFISIILLPIVGNAAEHAGAIIFALKNKLDITLGVALGSATQISMFVVPLS 366

Query: 370 VIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACFFV 429
           V+VAW MGV MDL+F L+ETGSL +A++ TAFTLQDGTSHY+KG++LLLCYI+I ACFFV
Sbjct: 367 VLVAWIMGVQMDLDFKLLETGSLFMAVLVTAFTLQDGTSHYLKGILLLLCYIVIGACFFV 426

Query: 430 SKTQEDNNNA 433
           ++    + N+
Sbjct: 427 ARQPAGHANS 434

BLAST of CsGy4G015200 vs. ExPASy Swiss-Prot
Match: Q5TKG3 (Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica OX=39947 GN=CAX1b PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 2.7e-138
Identity = 271/428 (63.32%), Postives = 330/428 (77.10%), Query Frame = 0

Query: 9   LMENGTL-KRLTKEHRHGHG----RTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCINLQ 68
           +ME+ T+ K +   H    G    RTAHN+SSSSLRK SD +L+ K+    LR    NL 
Sbjct: 7   MMESNTMAKEMAVRHHLLPGSPRRRTAHNLSSSSLRKSSDASLLHKVPCAALRSLLANLN 66

Query: 69  EVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPT 128
           +V+L T+L +LFPA+ LAI A    FG+ W+F LSL+GL PLAER+SFLTEQIA++TGPT
Sbjct: 67  DVLLTTRLFLLFPAVLLAIAATYLHFGQVWVFVLSLIGLVPLAERLSFLTEQIAFYTGPT 126

Query: 129 VGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIANIRR 188
           VGGLLNAT GN TE+IIA+LAL + KI+VVK SLLGS+LSNLLLVLGTSLF  GIAN+R 
Sbjct: 127 VGGLLNATFGNVTEVIIALLALREGKIEVVKCSLLGSILSNLLLVLGTSLFLAGIANLRA 186

Query: 189 EQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAAS---TLHLSRASSIVMLTA 248
            Q YD +QA VN+ +LML++LCH LPL+  YA  S   A  S    LHLSRA SI+ML A
Sbjct: 187 HQPYDTKQAHVNTALLMLAVLCHSLPLMLRYAVTSGDHAIVSGDAALHLSRACSILMLIA 246

Query: 249 YIAYLVFQLWTHRQLFEAEEGEDDD------VPGEEAVIGFWSAFAWLVGMTLVIALLSE 308
           Y+AYL FQL THRQLFE ++ EDDD         +E V+GF SA  WL  MTL+ ALLS 
Sbjct: 247 YLAYLFFQLNTHRQLFEPQQVEDDDDDDLVIAQDDEPVLGFSSAMIWLALMTLLTALLSG 306

Query: 309 YVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQI 368
           YVV+TIE AS +W LSVSF+SIILLPIVGNAAEHAGA+IFA KNK+DI+LGV+LGSATQI
Sbjct: 307 YVVSTIEAASESWELSVSFISIILLPIVGNAAEHAGAVIFALKNKMDITLGVSLGSATQI 366

Query: 369 ALFVIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYI 423
           ++FV+P+ VIVAWTMG+ MDL+FNL+ETGSL LAI+ TAFTLQ+G SHY+KGL+L+LCY 
Sbjct: 367 SMFVVPVSVIVAWTMGIPMDLDFNLLETGSLFLAILVTAFTLQEGESHYLKGLILVLCYA 426

BLAST of CsGy4G015200 vs. ExPASy Swiss-Prot
Match: Q945S5 (Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana OX=3702 GN=CAX4 PE=1 SV=2)

HSP 1 Score: 486.9 bits (1252), Expect = 2.5e-136
Identity = 253/408 (62.01%), Postives = 328/408 (80.39%), Query Frame = 0

Query: 28  RTAHNMSSSSL-RKKSDLTLVSKIQFGCLRKFCINLQEVILGTKLSILFPAIPLAIVAQN 87
           R    +S+SSL RK+SDL L+S++++  +R+   NLQEV+LGTKL ILFPA+PLA+VA  
Sbjct: 32  RVRRTVSASSLIRKRSDLKLISRVRWEFMRRILTNLQEVLLGTKLFILFPAVPLAVVAHR 91

Query: 88  FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPTVGGLLNATCGNATELIIAILALT 147
           +   R W+FALSLLGLTPLAER+SFLTEQIA+ TGPTVGGL+NATCGNATE+IIAILA+ 
Sbjct: 92  YDCPRAWVFALSLLGLTPLAERISFLTEQIAFHTGPTVGGLMNATCGNATEMIIAILAVG 151

Query: 148 QRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIANIRREQNYDRRQADVNSLMLMLSLLCH 207
           QRK+ +VK SLLGS+LSNLL VLGTSLF GGI+N+R+ Q++D RQ D+NS++L L+LLC 
Sbjct: 152 QRKMRIVKLSLLGSILSNLLFVLGTSLFLGGISNLRKHQSFDPRQGDMNSMLLYLALLCQ 211

Query: 208 LLPLLFGYAAASASTAAASTLHLSRASSIVMLTAYIAYLVFQLWTHR------QLFEAEE 267
            LP++  +   +     +  + LSRASS VML AY+A+L+F L++         L + E+
Sbjct: 212 TLPMIMRFTMEAEEYDGSDVVVLSRASSFVMLIAYLAFLIFHLFSSHLSPPPPPLPQRED 271

Query: 268 GEDDDVPGEE---AVIGFWSAFAWLVGMTLVIALLSEYVVNTIEEASTTWGLSVSFLSII 327
             DDDV  +E   AVIG WSA  WL+ MTL++ALLS+Y+V+TI++A+ +WGLSV F+ II
Sbjct: 272 VHDDDVSDKEEEGAVIGMWSAIFWLIIMTLLVALLSDYLVSTIQDAADSWGLSVGFIGII 331

Query: 328 LLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIPMCVIVAWTMGVDMDLNF 387
           LLPIVGNAAEHAGA+IFAF+NKLDI+LG+ALGSATQIALFV+P+ V+VAWTMG++MDLNF
Sbjct: 332 LLPIVGNAAEHAGAVIFAFRNKLDITLGIALGSATQIALFVVPVTVLVAWTMGIEMDLNF 391

Query: 388 NLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACFFVSKT 426
           NL+ET    L+I+ T+  LQDGTS+Y+KGLVLLLCY++IAACFFVS +
Sbjct: 392 NLLETACFALSILVTSLVLQDGTSNYMKGLVLLLCYVVIAACFFVSNS 439

BLAST of CsGy4G015200 vs. NCBI nr
Match: NP_001267681.1 (vacuolar cation/proton exchanger 1-like [Cucumis sativus] >XP_011652900.1 vacuolar cation/proton exchanger 1-like isoform X1 [Cucumis sativus] >AFQ99295.1 cation exchanger CAX3 [Cucumis sativus] >KGN54380.1 hypothetical protein Csa_018138 [Cucumis sativus])

HSP 1 Score: 855 bits (2210), Expect = 1.89e-312
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60
           MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180
           GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTA 240
           IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTA
Sbjct: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTA 240

Query: 241 YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTI 300
           YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTI
Sbjct: 241 YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTI 300

Query: 301 EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP 360
           EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP
Sbjct: 301 EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP 360

Query: 361 MCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF 420
           MCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF
Sbjct: 361 MCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF 420

Query: 421 FVSKTQEDNNNAPNLGLQSSNQAIFRA 447
           FVSKTQEDNNNAPNLGLQSSNQAIFRA
Sbjct: 421 FVSKTQEDNNNAPNLGLQSSNQAIFRA 447

BLAST of CsGy4G015200 vs. NCBI nr
Match: XP_008449925.1 (PREDICTED: vacuolar cation/proton exchanger 3 [Cucumis melo])

HSP 1 Score: 840 bits (2170), Expect = 2.35e-306
Identity = 437/447 (97.76%), Postives = 444/447 (99.33%), Query Frame = 0

Query: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60
           MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIP AIVA +FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPFAIVAHSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180
           GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTA 240
           +RREQ YDRRQADVNSLMLMLSLLCHLLPLLFGYAA+SASTAAASTLHLSRASSIVML+A
Sbjct: 181 LRREQIYDRRQADVNSLMLMLSLLCHLLPLLFGYAASSASTAAASTLHLSRASSIVMLSA 240

Query: 241 YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTI 300
           YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTL+IALLSEYVVNTI
Sbjct: 241 YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLLIALLSEYVVNTI 300

Query: 301 EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP 360
           EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP
Sbjct: 301 EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP 360

Query: 361 MCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF 420
           +CVIVAWTMGVDMDLNFN+METGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF
Sbjct: 361 LCVIVAWTMGVDMDLNFNIMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF 420

Query: 421 FVSKTQEDNNNAPNLGLQSSNQAIFRA 447
           FVSKTQEDNNNAPNLGLQSSNQAIFRA
Sbjct: 421 FVSKTQEDNNNAPNLGLQSSNQAIFRA 447

BLAST of CsGy4G015200 vs. NCBI nr
Match: XP_038882514.1 (vacuolar cation/proton exchanger 3-like [Benincasa hispida])

HSP 1 Score: 823 bits (2127), Expect = 8.39e-300
Identity = 430/447 (96.20%), Postives = 440/447 (98.43%), Query Frame = 0

Query: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60
           MASQTEPWLMENG+LKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQ GCLRKF I
Sbjct: 1   MASQTEPWLMENGSLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQLGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQ+FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180
           GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTA 240
           +RREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAA+S +TAAASTLHLSRASSIVML+A
Sbjct: 181 LRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAASSTATAAASTLHLSRASSIVMLSA 240

Query: 241 YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTI 300
           YIAYLVFQLWTHRQLFEAEE EDDDVPGEEAVIGFWSAFAWLVGMTL+IALLSEYVVNTI
Sbjct: 241 YIAYLVFQLWTHRQLFEAEEVEDDDVPGEEAVIGFWSAFAWLVGMTLLIALLSEYVVNTI 300

Query: 301 EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP 360
           EEAS+TWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP
Sbjct: 301 EEASSTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP 360

Query: 361 MCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF 420
           +CVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF
Sbjct: 361 LCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF 420

Query: 421 FVSKTQEDNNNAPNLGLQSSNQAIFRA 447
           FVSKTQED  NAPNL LQSSNQAIF+A
Sbjct: 421 FVSKTQEDGKNAPNLELQSSNQAIFKA 447

BLAST of CsGy4G015200 vs. NCBI nr
Match: XP_022949765.1 (vacuolar cation/proton exchanger 3-like [Cucurbita moschata] >KAG6603584.1 Vacuolar cation/proton exchanger 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 798 bits (2060), Expect = 1.41e-289
Identity = 421/450 (93.56%), Postives = 435/450 (96.67%), Query Frame = 0

Query: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQ+FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDVVK+SLLGSVLSNLLLVLGTSLFCGGI N
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASAS---TAAASTLHLSRASSIVM 240
             +EQ+YDRRQADVN+LMLMLSLLCHLLPLLFGYAA+SAS   TAAASTLHLSRASSIVM
Sbjct: 181 --KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240

Query: 241 LTAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVV 300
           L AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTL+IALLSEYVV
Sbjct: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420
           VIP+CVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 420

Query: 421 ACFFVSKTQEDNNNAPNLGLQSSNQAIFRA 447
           ACFFVSKTQED+NNAPNLGLQ+SNQ+IFRA
Sbjct: 421 ACFFVSKTQEDDNNAPNLGLQASNQSIFRA 448

BLAST of CsGy4G015200 vs. NCBI nr
Match: XP_023545142.1 (vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796 bits (2057), Expect = 4.03e-289
Identity = 421/450 (93.56%), Postives = 434/450 (96.44%), Query Frame = 0

Query: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQ+FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDVVK+SLLGSVLSNLLLVLGTSLFCGGI N
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASAS---TAAASTLHLSRASSIVM 240
             +EQ YDRRQADVN+LMLMLSLLCHLLPLLFGYAA+SAS   TAAASTLHLSRASSIVM
Sbjct: 181 --KEQTYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240

Query: 241 LTAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVV 300
           L AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTL+IALLSEYVV
Sbjct: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420
           VIP+CVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 420

Query: 421 ACFFVSKTQEDNNNAPNLGLQSSNQAIFRA 447
           ACFFVSKTQED+NNAPNLGLQSS+Q+IFRA
Sbjct: 421 ACFFVSKTQEDDNNAPNLGLQSSSQSIFRA 448

BLAST of CsGy4G015200 vs. ExPASy TrEMBL
Match: J7KJQ3 (Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX3 PE=2 SV=1)

HSP 1 Score: 855 bits (2210), Expect = 9.13e-313
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60
           MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180
           GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTA 240
           IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTA
Sbjct: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTA 240

Query: 241 YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTI 300
           YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTI
Sbjct: 241 YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTI 300

Query: 301 EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP 360
           EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP
Sbjct: 301 EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP 360

Query: 361 MCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF 420
           MCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF
Sbjct: 361 MCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF 420

Query: 421 FVSKTQEDNNNAPNLGLQSSNQAIFRA 447
           FVSKTQEDNNNAPNLGLQSSNQAIFRA
Sbjct: 421 FVSKTQEDNNNAPNLGLQSSNQAIFRA 447

BLAST of CsGy4G015200 vs. ExPASy TrEMBL
Match: A0A1S3BNT2 (Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103491652 PE=3 SV=1)

HSP 1 Score: 840 bits (2170), Expect = 1.14e-306
Identity = 437/447 (97.76%), Postives = 444/447 (99.33%), Query Frame = 0

Query: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60
           MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIP AIVA +FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPFAIVAHSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180
           GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTA 240
           +RREQ YDRRQADVNSLMLMLSLLCHLLPLLFGYAA+SASTAAASTLHLSRASSIVML+A
Sbjct: 181 LRREQIYDRRQADVNSLMLMLSLLCHLLPLLFGYAASSASTAAASTLHLSRASSIVMLSA 240

Query: 241 YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTI 300
           YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTL+IALLSEYVVNTI
Sbjct: 241 YIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLLIALLSEYVVNTI 300

Query: 301 EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP 360
           EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP
Sbjct: 301 EEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIP 360

Query: 361 MCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF 420
           +CVIVAWTMGVDMDLNFN+METGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF
Sbjct: 361 LCVIVAWTMGVDMDLNFNIMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACF 420

Query: 421 FVSKTQEDNNNAPNLGLQSSNQAIFRA 447
           FVSKTQEDNNNAPNLGLQSSNQAIFRA
Sbjct: 421 FVSKTQEDNNNAPNLGLQSSNQAIFRA 447

BLAST of CsGy4G015200 vs. ExPASy TrEMBL
Match: A0A6J1GDQ2 (Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111453055 PE=3 SV=1)

HSP 1 Score: 798 bits (2060), Expect = 6.82e-290
Identity = 421/450 (93.56%), Postives = 435/450 (96.67%), Query Frame = 0

Query: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQ+FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDVVK+SLLGSVLSNLLLVLGTSLFCGGI N
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASAS---TAAASTLHLSRASSIVM 240
             +EQ+YDRRQADVN+LMLMLSLLCHLLPLLFGYAA+SAS   TAAASTLHLSRASSIVM
Sbjct: 181 --KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240

Query: 241 LTAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVV 300
           L AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTL+IALLSEYVV
Sbjct: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420
           VIP+CVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 420

Query: 421 ACFFVSKTQEDNNNAPNLGLQSSNQAIFRA 447
           ACFFVSKTQED+NNAPNLGLQ+SNQ+IFRA
Sbjct: 421 ACFFVSKTQEDDNNAPNLGLQASNQSIFRA 448

BLAST of CsGy4G015200 vs. ExPASy TrEMBL
Match: A0A6J1ITD3 (Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111478234 PE=3 SV=1)

HSP 1 Score: 795 bits (2053), Expect = 7.94e-289
Identity = 419/450 (93.11%), Postives = 435/450 (96.67%), Query Frame = 0

Query: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQ+FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180
           GPTVGGLLNATCGNATE+IIAILALTQ KIDVVK+SLLGSVLSNLLLVLGTSLFCGGI N
Sbjct: 121 GPTVGGLLNATCGNATEMIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASAS---TAAASTLHLSRASSIVM 240
             +EQ+YDRRQADVN+LMLMLSLLCHLLPLLFGYAA+SAS   TAAASTLHLSRASSIVM
Sbjct: 181 --KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240

Query: 241 LTAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVV 300
           L AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTL+IALLSEYVV
Sbjct: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420
           VIP+CVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 420

Query: 421 ACFFVSKTQEDNNNAPNLGLQSSNQAIFRA 447
           ACFFVSKTQED+NNAPNLGLQ+S+Q+IFRA
Sbjct: 421 ACFFVSKTQEDDNNAPNLGLQASSQSIFRA 448

BLAST of CsGy4G015200 vs. ExPASy TrEMBL
Match: A0A6J1DEY3 (Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111019448 PE=3 SV=1)

HSP 1 Score: 773 bits (1997), Expect = 2.90e-280
Identity = 406/447 (90.83%), Postives = 431/447 (96.42%), Query Frame = 0

Query: 3   SQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCINL 62
           +QTEPWL+ENGTLKRLTK+ RHGHGRTAHNMSSSSLRKKSDLTLVSKI+F CLR F INL
Sbjct: 5   TQTEPWLLENGTLKRLTKDSRHGHGRTAHNMSSSSLRKKSDLTLVSKIRFPCLRNFLINL 64

Query: 63  QEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGP 122
           QEVILGTKLSILFPAIPLAI+AQ F FGRPW+FALSLLGLTPLAERVSFLTEQIAYFTGP
Sbjct: 65  QEVILGTKLSILFPAIPLAIIAQIFRFGRPWVFALSLLGLTPLAERVSFLTEQIAYFTGP 124

Query: 123 TVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIANIR 182
           TVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN+R
Sbjct: 125 TVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIANMR 184

Query: 183 REQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASASTAAASTLHLSRASSIVMLTAYI 242
            EQNYDRRQADVN+LMLMLSLLCHLLPLLFGYAA+SA+TAAASTLHLSRASSIVML+AYI
Sbjct: 185 TEQNYDRRQADVNALMLMLSLLCHLLPLLFGYAASSAATAAASTLHLSRASSIVMLSAYI 244

Query: 243 AYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVVNTIEE 302
           AYLVFQLWTHRQLFE E+G+DD V  EEAVIGF SAFAWL+GMT++IALLSEYVVN+IEE
Sbjct: 245 AYLVFQLWTHRQLFE-EDGDDDVVSDEEAVIGFGSAFAWLIGMTILIALLSEYVVNSIEE 304

Query: 303 ASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIPMC 362
           AST+WGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDI+LGVALGSATQIALFVIPMC
Sbjct: 305 ASTSWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDITLGVALGSATQIALFVIPMC 364

Query: 363 VIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACFFV 422
           VIVAWTMGVDMDLNFN+METGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIAACFFV
Sbjct: 365 VIVAWTMGVDMDLNFNIMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAACFFV 424

Query: 423 SKTQEDNNNAP--NLGLQSSNQAIFRA 447
           SKTQ D++NAP  N+G+QSSNQAIF A
Sbjct: 425 SKTQPDDSNAPIPNMGIQSSNQAIFTA 450

BLAST of CsGy4G015200 vs. TAIR 10
Match: AT3G51860.1 (cation exchanger 3 )

HSP 1 Score: 592.4 bits (1526), Expect = 3.0e-169
Identity = 327/455 (71.87%), Postives = 374/455 (82.20%), Query Frame = 0

Query: 1   MASQTEPW--LMENG----TLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGC 60
           M S  EPW  + ENG    T K  ++E R  HGRTAHNMSSSSLRKKSDL LV K+    
Sbjct: 1   MGSIVEPWAAIAENGNANVTAKGSSRELR--HGRTAHNMSSSSLRKKSDLRLVQKVPCKT 60

Query: 61  LRKFCINLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTE 120
           L+    NLQEVILGTKL++LF AIPLAI+A ++ +GRP IF LSL+GLTPLAERVSFLTE
Sbjct: 61  LKNILSNLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTE 120

Query: 121 QIAYFTGPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLF 180
           Q+A++TGPTVGGLLNATCGNATELIIAILAL   K+ VVKYSLLGS+LSNLLLVLGTSLF
Sbjct: 121 QLAFYTGPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLF 180

Query: 181 CGGIANIRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAA---ASASTAAASTLHLSR 240
            GGIANIRREQ +DR+QADVN  +L++ LLCHLLPLL  YAA    S S     +L LSR
Sbjct: 181 FGGIANIRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSR 240

Query: 241 ASSIVMLTAYIAYLVFQLWTHRQLFEAEEGE----DDDVPGEEA-VIGFWSAFAWLVGMT 300
            SSIVML AYIAYL+FQLWTHRQLFEA++ +    DD+V  EE  VIGFWS FAWLVGMT
Sbjct: 241 TSSIVMLIAYIAYLIFQLWTHRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMT 300

Query: 301 LVIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360
           +VIALLSEYVV+TIE+AS +WGLSVSF+SIILLPIVGNAAEHAGAIIFAFKNKLDISLGV
Sbjct: 301 IVIALLSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360

Query: 361 ALGSATQIALFVIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKG 420
           ALGSATQI+LFV+P+ VIVAW +G+ MDLNFN++ET SL LAII TAFTLQDGTSHY+KG
Sbjct: 361 ALGSATQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKG 420

Query: 421 LVLLLCYIIIAACFFVSKTQEDNNNAPNLGLQSSN 442
           LVLLLCY+IIAACFFV +  + N+   ++GLQ  N
Sbjct: 421 LVLLLCYVIIAACFFVDQIPQPND--LDVGLQPMN 451

BLAST of CsGy4G015200 vs. TAIR 10
Match: AT2G38170.1 (cation exchanger 1 )

HSP 1 Score: 587.8 bits (1514), Expect = 7.4e-168
Identity = 321/451 (71.18%), Postives = 365/451 (80.93%), Query Frame = 0

Query: 5   TEPW-LMENG----TLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFC 64
           TEPW + ENG    T K  ++E R   GRTAHNMSSSSLRKKSDL ++ K+ +  L+ F 
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIA 184
           TGPT+GGLLNATCGNATELIIAILALT  K+ VVKYSLLGS+LSNLLLVLGTSLFCGGIA
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 NIRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGY---AAASASTAAASTLHLSRASSIV 244
           NIRREQ +DR+QADVN  +L+L  LCHLLPLL GY     ASA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLTAYIAYLVFQLWTHRQLFEAEEGE---DDDVPGEEAVIGFWSAFAWLVGMTLVIALLS 304
           ML +YIAYLVFQLWTHRQLF+A+E E   DDDV  E AVI FWS FAWLVGMTLVIALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 364
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKLDISLGVALGSATQ
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQ 365

Query: 365 IALFVIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCY 424
           I LFV+P+ +IVAW +G++MDLNF  +ETG L ++II TAFTLQDG+SHY+KGLVLLLCY
Sbjct: 366 IGLFVVPLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCY 425

Query: 425 IIIAACFFVSKTQEDNNNAPNLGLQSSNQAI 445
            IIA CFFV K  +   NA +LG Q+ N  +
Sbjct: 426 FIIAICFFVDKLPQ-KQNAIHLGHQAMNNVV 453

BLAST of CsGy4G015200 vs. TAIR 10
Match: AT2G38170.3 (cation exchanger 1 )

HSP 1 Score: 580.1 bits (1494), Expect = 1.5e-165
Identity = 315/431 (73.09%), Postives = 355/431 (82.37%), Query Frame = 0

Query: 5   TEPW-LMENG----TLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFC 64
           TEPW + ENG    T K  ++E R   GRTAHNMSSSSLRKKSDL ++ K+ +  L+ F 
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIA 184
           TGPT+GGLLNATCGNATELIIAILALT  K+ VVKYSLLGS+LSNLLLVLGTSLFCGGIA
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 NIRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGY---AAASASTAAASTLHLSRASSIV 244
           NIRREQ +DR+QADVN  +L+L  LCHLLPLL GY     ASA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLTAYIAYLVFQLWTHRQLFEAEEGE---DDDVPGEEAVIGFWSAFAWLVGMTLVIALLS 304
           ML +YIAYLVFQLWTHRQLF+A+E E   DDDV  E AVI FWS FAWLVGMTLVIALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 364
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKLDISLGVALGSATQ
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQ 365

Query: 365 IALFVIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCY 424
           I LFV+P+ +IVAW +G++MDLNF  +ETG L ++II TAFTLQDG+SHY+KGLVLLLCY
Sbjct: 366 IGLFVVPLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCY 425

BLAST of CsGy4G015200 vs. TAIR 10
Match: AT5G01490.1 (cation exchanger 4 )

HSP 1 Score: 491.5 bits (1264), Expect = 7.2e-139
Identity = 255/417 (61.15%), Postives = 330/417 (79.14%), Query Frame = 0

Query: 28  RTAHNMSSSSL-RKKSDLTLVSKIQFGCLRKFCINLQEVILGTKLSILFPAIPLAIVAQN 87
           R    +S+SSL RK+SDL L+S++++  +R+   NLQEV+LGTKL ILFPA+PLA+VA  
Sbjct: 32  RVRRTVSASSLIRKRSDLKLISRVRWEFMRRILTNLQEVLLGTKLFILFPAVPLAVVAHR 91

Query: 88  FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPTVGGLLNATCGNATELIIAILALT 147
           +   R W+FALSLLGLTPLAER+SFLTEQIA+ TGPTVGGL+NATCGNATE+IIAILA+ 
Sbjct: 92  YDCPRAWVFALSLLGLTPLAERISFLTEQIAFHTGPTVGGLMNATCGNATEMIIAILAVG 151

Query: 148 QRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIANIRREQNYDRRQADVNSLMLMLSLLCH 207
           QRK+ +VK SLLGS+LSNLL VLGTSLF GGI+N+R+ Q++D RQ D+NS++L L+LLC 
Sbjct: 152 QRKMRIVKLSLLGSILSNLLFVLGTSLFLGGISNLRKHQSFDPRQGDMNSMLLYLALLCQ 211

Query: 208 LLPLLFGYAAASASTAAASTLHLSRASSIVMLTAYIAYLVFQLWTHR------QLFEAEE 267
            LP++  +   +     +  + LSRASS VML AY+A+L+F L++         L + E+
Sbjct: 212 TLPMIMRFTMEAEEYDGSDVVVLSRASSFVMLIAYLAFLIFHLFSSHLSPPPPPLPQRED 271

Query: 268 GEDDDVPGEE---AVIGFWSAFAWLVGMTLVIALLSEYVVNTIEEASTTWGLSVSFLSII 327
             DDDV  +E   AVIG WSA  WL+ MTL++ALLS+Y+V+TI++A+ +WGLSV F+ II
Sbjct: 272 VHDDDVSDKEEEGAVIGMWSAIFWLIIMTLLVALLSDYLVSTIQDAADSWGLSVGFIGII 331

Query: 328 LLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIPMCVIVAWTMGVDMDLNF 387
           LLPIVGNAAEHAGA+IFAF+NKLDI+LG+ALGSATQIALFV+P+ V+VAWTMG++MDLNF
Sbjct: 332 LLPIVGNAAEHAGAVIFAFRNKLDITLGIALGSATQIALFVVPVTVLVAWTMGIEMDLNF 391

Query: 388 NLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIAACFFVSKTQEDNNNAPN 435
           NL+ET    L+I+ T+  LQDGTS+Y+KGLVLLLCY++IAACFFVS +     N  N
Sbjct: 392 NLLETACFALSILVTSLVLQDGTSNYMKGLVLLLCYVVIAACFFVSNSPSTETNTTN 448

BLAST of CsGy4G015200 vs. TAIR 10
Match: AT2G38170.2 (cation exchanger 1 )

HSP 1 Score: 456.8 bits (1174), Expect = 2.0e-128
Identity = 254/351 (72.36%), Postives = 284/351 (80.91%), Query Frame = 0

Query: 5   TEPW-LMENG----TLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFC 64
           TEPW + ENG    T K  ++E R   GRTAHNMSSSSLRKKSDL ++ K+ +  L+ F 
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIA 184
           TGPT+GGLLNATCGNATELIIAILALT  K+ VVKYSLLGS+LSNLLLVLGTSLFCGGIA
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 NIRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGY---AAASASTAAASTLHLSRASSIV 244
           NIRREQ +DR+QADVN  +L+L  LCHLLPLL GY     ASA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLTAYIAYLVFQLWTHRQLFEAEEGE---DDDVPGEEAVIGFWSAFAWLVGMTLVIALLS 304
           ML +YIAYLVFQLWTHRQLF+A+E E   DDDV  E AVI FWS FAWLVGMTLVIALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISL 345
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKL  S+
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLVSSI 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z814.2e-16871.87Vacuolar cation/proton exchanger 3 OS=Arabidopsis thaliana OX=3702 GN=CAX3 PE=1 ... [more]
Q392531.0e-16671.18Vacuolar cation/proton exchanger 1 OS=Arabidopsis thaliana OX=3702 GN=CAX1 PE=1 ... [more]
Q769E57.5e-14966.51Vacuolar cation/proton exchanger 1a OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q5TKG32.7e-13863.32Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q945S52.5e-13662.01Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana OX=3702 GN=CAX4 PE=1 ... [more]
Match NameE-valueIdentityDescription
NP_001267681.11.89e-312100.00vacuolar cation/proton exchanger 1-like [Cucumis sativus] >XP_011652900.1 vacuol... [more]
XP_008449925.12.35e-30697.76PREDICTED: vacuolar cation/proton exchanger 3 [Cucumis melo][more]
XP_038882514.18.39e-30096.20vacuolar cation/proton exchanger 3-like [Benincasa hispida][more]
XP_022949765.11.41e-28993.56vacuolar cation/proton exchanger 3-like [Cucurbita moschata] >KAG6603584.1 Vacuo... [more]
XP_023545142.14.03e-28993.56vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
J7KJQ39.13e-313100.00Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX3 PE=2 SV=1[more]
A0A1S3BNT21.14e-30697.76Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103491652 PE=3 SV... [more]
A0A6J1GDQ26.82e-29093.56Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111453055 P... [more]
A0A6J1ITD37.94e-28993.11Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111478234 PE=... [more]
A0A6J1DEY32.90e-28090.83Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111019448 ... [more]
Match NameE-valueIdentityDescription
AT3G51860.13.0e-16971.87cation exchanger 3 [more]
AT2G38170.17.4e-16871.18cation exchanger 1 [more]
AT2G38170.31.5e-16573.09cation exchanger 1 [more]
AT5G01490.17.2e-13961.15cation exchanger 4 [more]
AT2G38170.22.0e-12872.36cation exchanger 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044880NCX, central ion-binding domain superfamilyGENE3D1.20.1420.30coord: 273..422
e-value: 8.6E-13
score: 50.2
coord: 122..272
e-value: 9.5E-15
score: 56.6
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 93..250
e-value: 8.9E-23
score: 80.9
coord: 278..419
e-value: 1.5E-16
score: 60.7
NoneNo IPR availableTIGRFAMTIGR00846TIGR00846coord: 58..421
e-value: 2.3E-146
score: 485.3
NoneNo IPR availablePANTHERPTHR31503:SF1VACUOLAR CATION/PROTON EXCHANGER 3coord: 4..434
IPR004798Calcium/proton exchanger CAX-likeTIGRFAMTIGR00378TIGR00378coord: 76..421
e-value: 5.2E-96
score: 319.8
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 4..434

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G015200.2CsGy4G015200.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0006874 cellular calcium ion homeostasis
biological_process GO:0006816 calcium ion transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity