CsGy4G013043 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G013043
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionLipoxygenase
LocationGy14Chr4: 18173043 .. 18175690 (+)
RNA-Seq ExpressionCsGy4G013043
SyntenyCsGy4G013043
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTATAAGGGTACTGTAACTGTTACTGTGAATCCCAAAAAGAACGAAGCCTTTCTTACGACCGAAGTCATATATCTCGAGTTTGTTAGCGTCGAATTGGATTCGAGTAAATCCTCATCTTCTCTCTTTATTTATTTATATATGATTTTCTACACTGCTAATGTAACCTATATATATTCTTCTAATGTAAAGTGATTTAATAATGCACATATGACATGTACATATACACAGAAGGAAATCAACAGATTATCGGATGTACGGCTGGATTAAAATGTGGTGGTGACGTGAGCAAGCTGTTTGAAACAAGTATTGATGTTCCTGGAGATTTCGGGGAGATTGGAGCAGTGATTGTTGAATTGGATGATGATGTGAAAGAGAGGTTTATCGACACCATTACTATTGATTTCAAGAAGTGTACCTCGTTGACGTTTAGCTGCAAATCATGGGTGCAACCCAAAAGTAGTACTCACCAACGAAGGATCTTCTTTTCGTCCAACAAGGTGATGCTTTGCTTCATTATTTAATTTATATAAATTAATTAATACTCAAACCCCATTATTCTCAATCCACATAAATATTGAGTAATTCAAATTTCAGTGGATATTTTTTAAGAAAATTATAAAAATGAAAATTAATTATTAAATAAAACATTTTATTATGTATTATTTCAAAAAGCATAGCAAAAAGAAGTAAAGCATTTACAAAATGCAACAAATTATCATACTTTGTCACCCATTTATTAGAGCGTAAATTGTGATATTTTGTTATATTTATAAATATTTTCATAACTTGTGTCATTTAAAATAATTGTTTTTTATTAATTTCAAATAAGTAAACATTTTACTATTTATTCTACTTTTTTTTTTTTTGTTGTGTTTTGCGTATTTTTCTACGTATAATAGATATATTAAATTCATGTTTGGTATCAATATCAAACCAAAATTCATGTTTGGTATCAATATCAAACCTAAGCAAACGATGAAGTATGGATGAAAATATTGAGATGCAGATGGAAATTTAATATGATTTTGTGAGGCTAATTAATAATTGAATATGAGGATGAATGCAGTCTTATTTGTCGGGAAAAACACCAGAAGGACTGGTGAAGCTGAGAGCAGAAGACCTTGCAAATCTGAGAGGCGAAAAAGAAGATGGATCGGTAGATAAAAAGAAGCGTAATGCATTTGAAAGAATTTATGATTACGATCTGTACAACGACCTCGGAAAACCCGACGAATCATCAGATTTGATAAGGCCTGTTCTTGGCGGTTCAGAGGAGTATCCTTATCCTCGACGTTGTAGAACTGGTCGTGATACTAGTGACAATGGTATCTTAATTTTCTAAATCATTTCTTGTTAATTTAATTGTACAATACATACATGCGTATTTATTAATGGATTCATTTGGCCGTACTATTGGATTTAGATAAACATGCAGAGGAAAGGATTGAGGCACCTTTTTATGTTCCACGAGACGAAAACTTTTCAGAGGAAAAGCAAGGAAGTGTTTTAGTAGAAAACGAGGCATTGCTGGGAAAAGCTCCTTTCTCTGACCTCCCTGAACTTGACATTAAAACGCCTTTCGCTGCTTCCAAAATTAATCAACTTCCATTTGATATACCATCCCTAGTTTCCTCTAATCAACCACCTCCTCTTAATTCATTAGACGATGAACCCAGTAGTTCCGTTGAACTTCCACTACCAGAGTCCTACAAGAGTGAGTATATATGTTTATGATAAGACACTTCGATTTCAAAGTGCATCCATGTGGCAAACACTAACTAACTAATATGCAAAAACCTTTTTCGTTTGATTATTATACTTCATTTTCTCAGGAGACAAATACAATTGGCTGAGTGACATTGAATTTGCTAGACTCACACTTGCTGGTCTAAACCCTTACTCTATACAGCTAGTCAAAGTATGTGACAATTAACTCAAAATCAATTAATAATATATAACTTTATAAATATGGATTAATCTCTATAATTTAATTATTCTCCTGATAATAATTGATCAGAGTTTGCCGTTTATGAGTAAGCTTGACGAGGGAGTTTATGGACCTCGAGAATCAAAATTCACTCATGAACTAGTTCAAGAATTACTTGGATGTCGCATCAAAGTTTGCAAGGTGAAATAAATTAGCTCAATGCAGTAATTTATTAAATTAGAACTTAAATAAAAAAATGTGATTAAATTTTTGTGAATTTATGCAGGCATTAACCGATAAAAGATTATTTGTATTGGATTACCATGATATTTTAATGCCATATGTAAGCAAAGTAAGAAAAATTGAGTGGACGACACTATATGGATCAAGGACCTTGTTCTTTCTAAACTCCGATGACACTTTGGAGCCACTGGGAATTGAGCTGACTCGACCTCCAATCAAAGGACATTCTGAACAATGGAAAGAAGTTTTCACACCCGGCACAAATTCTACAGATGTATGGATATGGAGGCTTGCGAAAGCCCATGTCCTTTCTCACGATTCTTGCATTCATCAACTTGTTATTCACTGGTATGTATATTCATACCTATATACATACATACATGCATGCATATATTTATTATCAAATATGATAATTGTTATACAGGCTGAGAGCTCATTGTTGTATGGAGCCGTATGCCATCGCCACACATAG

mRNA sequence

ATGAGTTATAAGGGTACTGTAACTGTTACTGTGAATCCCAAAAAGAACGAAGCCTTTCTTACGACCGAAGTCATATATCTCGAGTTTGTTAGCGTCGAATTGGATTCGAAAGGAAATCAACAGATTATCGGATGTACGGCTGGATTAAAATGTGGTGGTGACGTGAGCAAGCTGTTTGAAACAAGTATTGATGTTCCTGGAGATTTCGGGGAGATTGGAGCAGTGATTGTTGAATTGGATGATGATGTGAAAGAGAGGTTTATCGACACCATTACTATTGATTTCAAGAAGTGTACCTCGTTGACGTTTAGCTGCAAATCATGGGTGCAACCCAAAAGTAGTACTCACCAACGAAGGATCTTCTTTTCGTCCAACAAGTCTTATTTGTCGGGAAAAACACCAGAAGGACTGGTGAAGCTGAGAGCAGAAGACCTTGCAAATCTGAGAGGCGAAAAAGAAGATGGATCGGTAGATAAAAAGAAGCGTAATGCATTTGAAAGAATTTATGATTACGATCTGTACAACGACCTCGGAAAACCCGACGAATCATCAGATTTGATAAGGCCTGTTCTTGGCGGTTCAGAGGAGTATCCTTATCCTCGACGTTGTAGAACTGGTCGTGATACTAGTGACAATGATAAACATGCAGAGGAAAGGATTGAGGCACCTTTTTATGTTCCACGAGACGAAAACTTTTCAGAGGAAAAGCAAGGAAGTGTTTTAGTAGAAAACGAGGCATTGCTGGGAAAAGCTCCTTTCTCTGACCTCCCTGAACTTGACATTAAAACGCCTTTCGCTGCTTCCAAAATTAATCAACTTCCATTTGATATACCATCCCTAGTTTCCTCTAATCAACCACCTCCTCTTAATTCATTAGACGATGAACCCAGTAGTTCCGTTGAACTTCCACTACCAGAGTCCTACAAGAGAGACAAATACAATTGGCTGAGTGACATTGAATTTGCTAGACTCACACTTGCTGGTCTAAACCCTTACTCTATACAGCTAGTCAAAAGTTTGCCGTTTATGAGTAAGCTTGACGAGGGAGTTTATGGACCTCGAGAATCAAAATTCACTCATGAACTAGTTCAAGAATTACTTGGATGTCGCATCAAAGTTTGCAAGGCATTAACCGATAAAAGATTATTTGTATTGGATTACCATGATATTTTAATGCCATATGTAAGCAAAGTAAGAAAAATTGAGTGGACGACACTATATGGATCAAGGACCTTGTTCTTTCTAAACTCCGATGACACTTTGGAGCCACTGGGAATTGAGCTGACTCGACCTCCAATCAAAGGACATTCTGAACAATGGAAAGAAGTTTTCACACCCGGCACAAATTCTACAGATGTATGGATATGGAGGCTTGCGAAAGCCCATGTCCTTTCTCACGATTCTTGCATTCATCAACTTGTTATTCACTGGTATGTATATTCATACCTATATACATACATACATGCATGCATATATTTATTATCAAATATGATAATTGTTATACAGGCTGAGAGCTCATTGTTGTATGGAGCCGTATGCCATCGCCACACATAG

Coding sequence (CDS)

ATGAGTTATAAGGGTACTGTAACTGTTACTGTGAATCCCAAAAAGAACGAAGCCTTTCTTACGACCGAAGTCATATATCTCGAGTTTGTTAGCGTCGAATTGGATTCGAAAGGAAATCAACAGATTATCGGATGTACGGCTGGATTAAAATGTGGTGGTGACGTGAGCAAGCTGTTTGAAACAAGTATTGATGTTCCTGGAGATTTCGGGGAGATTGGAGCAGTGATTGTTGAATTGGATGATGATGTGAAAGAGAGGTTTATCGACACCATTACTATTGATTTCAAGAAGTGTACCTCGTTGACGTTTAGCTGCAAATCATGGGTGCAACCCAAAAGTAGTACTCACCAACGAAGGATCTTCTTTTCGTCCAACAAGTCTTATTTGTCGGGAAAAACACCAGAAGGACTGGTGAAGCTGAGAGCAGAAGACCTTGCAAATCTGAGAGGCGAAAAAGAAGATGGATCGGTAGATAAAAAGAAGCGTAATGCATTTGAAAGAATTTATGATTACGATCTGTACAACGACCTCGGAAAACCCGACGAATCATCAGATTTGATAAGGCCTGTTCTTGGCGGTTCAGAGGAGTATCCTTATCCTCGACGTTGTAGAACTGGTCGTGATACTAGTGACAATGATAAACATGCAGAGGAAAGGATTGAGGCACCTTTTTATGTTCCACGAGACGAAAACTTTTCAGAGGAAAAGCAAGGAAGTGTTTTAGTAGAAAACGAGGCATTGCTGGGAAAAGCTCCTTTCTCTGACCTCCCTGAACTTGACATTAAAACGCCTTTCGCTGCTTCCAAAATTAATCAACTTCCATTTGATATACCATCCCTAGTTTCCTCTAATCAACCACCTCCTCTTAATTCATTAGACGATGAACCCAGTAGTTCCGTTGAACTTCCACTACCAGAGTCCTACAAGAGAGACAAATACAATTGGCTGAGTGACATTGAATTTGCTAGACTCACACTTGCTGGTCTAAACCCTTACTCTATACAGCTAGTCAAAAGTTTGCCGTTTATGAGTAAGCTTGACGAGGGAGTTTATGGACCTCGAGAATCAAAATTCACTCATGAACTAGTTCAAGAATTACTTGGATGTCGCATCAAAGTTTGCAAGGCATTAACCGATAAAAGATTATTTGTATTGGATTACCATGATATTTTAATGCCATATGTAAGCAAAGTAAGAAAAATTGAGTGGACGACACTATATGGATCAAGGACCTTGTTCTTTCTAAACTCCGATGACACTTTGGAGCCACTGGGAATTGAGCTGACTCGACCTCCAATCAAAGGACATTCTGAACAATGGAAAGAAGTTTTCACACCCGGCACAAATTCTACAGATGTATGGATATGGAGGCTTGCGAAAGCCCATGTCCTTTCTCACGATTCTTGCATTCATCAACTTGTTATTCACTGGTATGTATATTCATACCTATATACATACATACATGCATGCATATATTTATTATCAAATATGATAATTGTTATACAGGCTGAGAGCTCATTGTTGTATGGAGCCGTATGCCATCGCCACACATAG

Protein sequence

MSYKGTVTVTVNPKKNEAFLTTEVIYLEFVSVELDSKGNQQIIGCTAGLKCGGDVSKLFETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCTSLTFSCKSWVQPKSSTHQRRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLGKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQGSVLVENEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDDEPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIHQLVIHWYVYSYLYTYIHACIYLLSNMIIVIQAESSLLYGAVCHRHT*
Homology
BLAST of CsGy4G013043 vs. ExPASy Swiss-Prot
Match: R9WS04 (Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 5.1e-101
Identity = 223/500 (44.60%), Postives = 293/500 (58.60%), Query Frame = 0

Query: 5   GTVTVTVNPKKNEAFLTTEVIYLEFVSVELDSKGNQQ-IIGCTAGLKCGGDVSKL--FET 64
           GTV  +V+     +FL      LE VS +LDS G ++  +   A        SKL  ++ 
Sbjct: 93  GTVADSVSDFLGRSFL------LELVSSDLDSSGKEKDTVKAYATYDELDKESKLYKYQC 152

Query: 65  SIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCTSLTFSCKSWVQPKSSTHQRRIF 124
             +VP DFGEIGAV+V+ ++  ++ ++  I +D      +TF+C SW+  K     +RIF
Sbjct: 153 EFEVPDDFGEIGAVLVQ-NERHRDAYVKNIVLD----EIVTFTCDSWIHSKFDNPDKRIF 212

Query: 125 FSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLGKPD 184
           F  NKSYL  +TPEGL  LR +DL +LRG  E       +R +F+RIYDYD YND+G PD
Sbjct: 213 F-LNKSYLPSETPEGLKSLRQKDLESLRGNGEG------ERQSFDRIYDYDTYNDIGDPD 272

Query: 185 ESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQ---- 244
             SD+ RPVLGG+ E+P+PRRCRTGR  +  +  +E R   PFYVPRDE+F+E KQ    
Sbjct: 273 TDSDMARPVLGGN-EHPFPRRCRTGRKMTSTEPWSESRTTLPFYVPRDEDFAEIKQITRG 332

Query: 245 ---------------GSVLVENEALLGKAPFSDLPEL-----DIKTPFAASKINQLPFDI 304
                           SVL + +   G   F D+  L     DI  P  +  ++ LP  +
Sbjct: 333 ATTLYSVLHGVIPALSSVLKDEDK--GFPLFRDIELLYEKGVDIDPPPDSGTLSALPRLV 392

Query: 305 PSLVSSNQPPPLNSLDDEPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLV 364
            ++ +S +              ++   P    +D ++W  D EF R TLAGLNPYSIQLV
Sbjct: 393 KAITNSTK------------KVLQFETPRIKHKDSFSWFRDEEFCRQTLAGLNPYSIQLV 452

Query: 365 KSLPFMSKLDEGVYGPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSK 424
              P MSKLD  VYGP ES  T E V+E +   +   +AL  KRLF+LDYHD+L+PYV+K
Sbjct: 453 TEWPLMSKLDPEVYGPAESAITKETVEEEIKGFMTXEEALEQKRLFLLDYHDLLLPYVNK 512

Query: 425 VRKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWR 478
           VR+IE TTLYGSRTL FL    TL PL IELTRPP  G   QWK V+TP  ++TD W+W+
Sbjct: 513 VREIEGTTLYGSRTLMFLTCTGTLRPLAIELTRPPNNG-KPQWKHVYTPCWDATDXWLWK 558

BLAST of CsGy4G013043 vs. ExPASy Swiss-Prot
Match: O24370 (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 7.2e-95
Identity = 209/476 (43.91%), Postives = 280/476 (58.82%), Query Frame = 0

Query: 25  IYLEFVSVELDSKGNQQIIGCTAGLKCGGDV--SKLFETSIDVPGDFGEIGAVIVELDDD 84
           + L  V+ ELD K   +  G  A    G DV     +E    +P DFGE+GA+++E ++ 
Sbjct: 101 LLLWIVAAELDHKTGIEKPGIRAYAHRGRDVDGDTHYEADFVIPQDFGEVGAILIE-NEH 160

Query: 85  VKERFIDTITIDFKKCTSLTFSCKSWVQPKSSTHQRRIFFSSNKSYLSGKTPEGLVKLRA 144
            KE ++  I ID      +  +C SWV  K     +RIFF +NKSYL  +TP G+ +LR 
Sbjct: 161 HKEMYVKNIVIDGFVHGKVEITCNSWVHSKFDNPDKRIFF-TNKSYLPSQTPSGVSRLRE 220

Query: 145 EDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLGKPDESS-DLIRPVLGGSEEYPYPR 204
           E+L  LRG   DG  ++K    FERIYDYD+YNDLG+ D ++ D  RPVLGG +E PYPR
Sbjct: 221 EELVTLRG---DGIGERK---VFERIYDYDVYNDLGEADSNNDDAKRPVLGG-KELPYPR 280

Query: 205 RCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQGSVLVENEALLGKAPFSDL----- 264
           RC+TGR  S  D  +E R     YVPRDE FSE       V++ A  G   +S L     
Sbjct: 281 RCKTGRPRSKKDPLSETR-STFVYVPRDEAFSE-------VKSVAFSGNTVYSVLHAVVP 340

Query: 265 --------PELDIKTPFAASKINQLPFDIPSLVSSNQ------PPPLNSLDDEPSSSVEL 324
                   P L      A   +  +  D+P L           P  + ++ D     +  
Sbjct: 341 ALESVVTDPNLGFPHFPAIDSLFNVGVDLPGLGDKKSGLFNVVPRLIKAISDTRKDVLLF 400

Query: 325 PLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPRESKFTHEL 384
             P+  +RDK++W  D+EFAR TLAGLNPYSI+LV   P  SKLD  VYGP ES+ T EL
Sbjct: 401 ESPQLVQRDKFSWFRDVEFARQTLAGLNPYSIRLVTEWPLRSKLDPKVYGPPESEITKEL 460

Query: 385 VQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFFLNSDDTLE 444
           +++ +G  + V +A+  K+LF+LDYHD+L+PYV+KV +++ + LYGSRT+FFL    TL+
Sbjct: 461 IEKEIGNYMTVEQAVQQKKLFILDYHDLLLPYVNKVNELKGSMLYGSRTIFFLTPQGTLK 520

Query: 445 PLGIELTRPPIKGHSEQWKEVFTPGT-NSTDVWIWRLAKAHVLSHDSCIHQLVIHW 478
           PL IELTRPP+     QWKEV++P   N+T  W+W+LAKAHVLSHDS  HQLV HW
Sbjct: 521 PLAIELTRPPV-DDKPQWKEVYSPNDWNATGAWLWKLAKAHVLSHDSGYHQLVSHW 558

BLAST of CsGy4G013043 vs. ExPASy Swiss-Prot
Match: P38419 (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 347.1 bits (889), Expect = 3.6e-94
Identity = 223/531 (42.00%), Postives = 307/531 (57.82%), Query Frame = 0

Query: 6   TVTVTVNPKKNEAF----LTTEVIYLEFVSVELDSK-GNQQIIGCTAGLKCGGDVSKL-- 65
           T+ VTV    N +     L    + LE VS ELD+K G ++    +       D   +  
Sbjct: 91  TIKVTVGELINRSIDIRDLIGRSLSLELVSSELDAKTGKEKATVRSYAHNVDDDDHSVVT 150

Query: 66  FETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFK----KCTSLTFSCKSWVQPKS- 125
           +E   DVP  FG IGA+IV  ++  +E F++ I +         T L   C SWVQPKS 
Sbjct: 151 YEADFDVPSGFGPIGAIIV-TNELRQEMFLEDINLTASDGAGNSTVLPIRCNSWVQPKSV 210

Query: 126 ---STHQRRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYD 185
               T  +RIFF +NK+YL G+TP GL   R  DL   RG   DG+    +R A +R+YD
Sbjct: 211 GDEGTPSKRIFF-ANKTYLPGQTPAGLRSYRKNDLQQKRG---DGT---GEREADDRVYD 270

Query: 186 YDLYNDLGKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDE 245
           YD+YNDLG PD + DL RPVLGG++++PYPRRCRTGR  S  D  +E R +   YVPRDE
Sbjct: 271 YDVYNDLGNPDSNGDLARPVLGGNKQFPYPRRCRTGRPPSKKDPKSETR-KGNVYVPRDE 330

Query: 246 NFSEEKQ--------GSVLVENEALLGKAPFSDLPELDIKTPFAA----SKINQLPFDIP 305
            FS EK+        GSVL   +A +  A    L +L    PF +     K+ +   ++P
Sbjct: 331 EFSPEKEDYFLRKTVGSVL---QAAVPAAQSLLLDKLKWNLPFPSFFVIDKLFEDGVELP 390

Query: 306 -----SLVSSNQPPPLNSLDDEPSSSV-ELPLPESYKRDKYNWLSDIEFARLTLAGLNPY 365
                + + S  P  L  L D P+  +     P + ++DK+ WL D EFAR TLAG+NPY
Sbjct: 391 GVDKLNFLESVVPRLLEHLRDTPAEKILRFETPANIQKDKFAWLRDEEFARETLAGINPY 450

Query: 366 SIQLVKSLPFMSKLDEGVYGPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILM 425
           +I+LV+  P  SKLD  VYGP ES  T +L++E +   + V +A++ KRLF+LD+HD+ +
Sbjct: 451 AIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFL 510

Query: 426 PYVSKVRKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTD 485
           PYV K+R ++ TT+YGSRT+FFL  D TL+ L IELTR P      QW++VFTP T++T 
Sbjct: 511 PYVHKIRSLDHTTMYGSRTVFFLTDDGTLQLLAIELTR-PASPSQPQWRQVFTPSTDATM 570

Query: 486 VWIWRLAKAHVLSHDSCIHQLVIHWY-VYSYLYTYIHACIYLLSNMIIVIQ 503
            W+WR+AKAHV +HD+  H+L+ HW   +  +  YI A    LS M  + Q
Sbjct: 571 SWLWRMAKAHVRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMHPIYQ 608

BLAST of CsGy4G013043 vs. ExPASy Swiss-Prot
Match: Q84YK8 (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.0e-93
Identity = 213/505 (42.18%), Postives = 296/505 (58.61%), Query Frame = 0

Query: 25  IYLEFVSVELDSK-GNQQIIGCTAGLKCGGDVSKL--FETSIDVPGDFGEIGAVIVELDD 84
           ++LE VS EL++K G ++    +   K   D   +  +E   DVP  FG IGAV+V  ++
Sbjct: 132 LFLELVSSELEAKTGKKKATVHSYAHKVDDDDHGVVTYEADFDVPTGFGPIGAVVV-TNE 191

Query: 85  DVKERFIDTITI----DFKKCTSLTFSCKSWVQPKSS----THQRRIFFSSNKSYLSGKT 144
             +E F++ + +         T L   C SWVQPKSS    T  +RIFF+  K+YL G+T
Sbjct: 192 LGQEMFLEDLNLTAGDGAGNSTVLPIRCNSWVQPKSSIDEGTPGKRIFFA--KAYLPGQT 251

Query: 145 PEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLGKPDESSDLIRPVLGG 204
           P GL   R EDL   RG          +R A +R+YDYD+YNDLG PD + DL RPVLGG
Sbjct: 252 PAGLRSYREEDLKQKRGN------GAGQREADDRVYDYDVYNDLGNPDSNGDLARPVLGG 311

Query: 205 SEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQGSVLVEN-----EALL 264
           S+++PYPRRCRTGR  S  D  +E R +   YVPRDE FSE K    L++       A +
Sbjct: 312 SKQFPYPRRCRTGRPPSKKDPKSETR-KGNVYVPRDEEFSEVKNAQFLLKTLQSVLHAAV 371

Query: 265 GKAPFSDLPELDIKTPFAA----SKINQLPFDIPSL-----VSSNQPPPLNSLDDEPSSS 324
             A  + +  L +  PF +     K+ +   ++P +     + S  P  L  L D P   
Sbjct: 372 PAAQSALIDNLSLNLPFPSFFVIDKLFEDGVELPGVEKLGFLHSIVPRLLELLRDSPGDK 431

Query: 325 VEL-PLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPRESKF 384
           + L   P + ++DK+ WL D EFAR TLAG+NPY+I+LV+  P  SKLD  VYGP ES  
Sbjct: 432 ILLFDTPANVQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAI 491

Query: 385 THELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFFLNSD 444
           T +L++E +   + V +A++ KRLF+LD+HD+ +PYV K+R ++ TT+YGSRT+FFL  D
Sbjct: 492 TADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSLKHTTMYGSRTIFFLTDD 551

Query: 445 DTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIHQLVIHWY 503
            TL  L IELTR P      QW++VFTP T++T  W+WR+AKAHV +HD+  H+L+ HW 
Sbjct: 552 GTLRLLAIELTR-PASPSQPQWRQVFTPSTDTTKSWLWRMAKAHVRAHDAGHHELITHWL 611

BLAST of CsGy4G013043 vs. ExPASy Swiss-Prot
Match: P38418 (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 4.3e-92
Identity = 196/439 (44.65%), Postives = 264/439 (60.14%), Query Frame = 0

Query: 59  FETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCTSLTFSCKSWVQPKSSTHQR 118
           +E   ++P DFG +GA+ ++ +   ++ F+  + +      S+TF+C+SWV PKS    +
Sbjct: 137 YECEFEMPEDFGPVGAIKIQ-NQYHRQLFLKGVELKLPG-GSITFTCESWVAPKSVDPTK 196

Query: 119 RIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLG 178
           RIFF S+KSYL  +TPE L K R E+L  L+G+  +   +  K   FERIYDYD+YND+G
Sbjct: 197 RIFF-SDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEFTK---FERIYDYDVYNDVG 256

Query: 179 KPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQG 238
            PD   +L RPV+GG   +PYPRRC+TGR   + D  +E+R    FYVPRDE FS  K  
Sbjct: 257 DPDNDPELARPVIGGL-THPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEFSTAK-- 316

Query: 239 SVLVENEALLGKAPFSDLPE---------LDIKTPFAASKI--------NQLPFD---IP 298
                  +  GKA  + LP          L  + PF   K          QLP D   +P
Sbjct: 317 -----GTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKDAGLLP 376

Query: 299 SLVSSNQPPPLNSLDDEPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVK 358
            L     P  + +L +     ++   P    RD+++WL D EFAR TLAGLNPYSIQLV+
Sbjct: 377 LL-----PRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVE 436

Query: 359 SLPFMSKLDEGVYGPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKV 418
             P +SKLD  VYG   S  T E+V+  +   + V +AL +KRLFVLDYHD+L+PYV+KV
Sbjct: 437 EWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKV 496

Query: 419 RKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRL 478
           R++  TTLY SRTLFFL+ D TL P+ IELT PP   +  QWK+VFTPG ++T  W+W L
Sbjct: 497 RELNNTTLYASRTLFFLSDDSTLRPVAIELTCPP-NINKPQWKQVFTPGYDATSCWLWNL 555

BLAST of CsGy4G013043 vs. NCBI nr
Match: KAE8649495.1 (hypothetical protein Csa_018136 [Cucumis sativus])

HSP 1 Score: 1034 bits (2673), Expect = 0.0
Identity = 513/519 (98.84%), Postives = 514/519 (99.04%), Query Frame = 0

Query: 1   MSYKGTVTVTVNPKKNEAFLTTEVIYLEFVSVELDSKGNQQIIGCTAGLKCGGDVSKLFE 60
           MSYKGTVTVTVNPKKNEAFLT EVIYLEFVSVELDSKGNQQIIGCTAGLKCGGDVSKLFE
Sbjct: 1   MSYKGTVTVTVNPKKNEAFLTIEVIYLEFVSVELDSKGNQQIIGCTAGLKCGGDVSKLFE 60

Query: 61  TSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCTSLTFSCKSWVQPKSSTHQRRI 120
           TSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCT LTFSCKSWVQPKSSTHQRRI
Sbjct: 61  TSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCTLLTFSCKSWVQPKSSTHQRRI 120

Query: 121 FFSSNK--SYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLG 180
           FFSSNK  SYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLG
Sbjct: 121 FFSSNKVMSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLG 180

Query: 181 KPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQG 240
           KPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQG
Sbjct: 181 KPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQG 240

Query: 241 SVLVENEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDDEPSS 300
           SVL ENEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDDEPSS
Sbjct: 241 SVLAENEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDDEPSS 300

Query: 301 SVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPRESKF 360
           SVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPRESKF
Sbjct: 301 SVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPRESKF 360

Query: 361 THELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFFLNSD 420
           THELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFFLNSD
Sbjct: 361 THELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFFLNSD 420

Query: 421 DTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIHQLVIHWY 480
           DTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVW+WRLAKAHVLSHDSCIHQLVIHWY
Sbjct: 421 DTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIHQLVIHWY 480

Query: 481 VYSYLYTYIHACIYLLSNMIIVIQAESSLLYGAVCHRHT 517
           VYSYLYTYIHACIYLLSNMIIVIQAESSLLYGAVCHRHT
Sbjct: 481 VYSYLYTYIHACIYLLSNMIIVIQAESSLLYGAVCHRHT 519

BLAST of CsGy4G013043 vs. NCBI nr
Match: XP_031740428.1 (LOW QUALITY PROTEIN: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 889 bits (2297), Expect = 0.0
Identity = 437/444 (98.42%), Postives = 440/444 (99.10%), Query Frame = 0

Query: 36  SKGNQQIIGCTAGLKCGGDVSKLFETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDF 95
           ++GNQQIIGCTAGLKCGGDVSKLFETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDF
Sbjct: 5   TEGNQQIIGCTAGLKCGGDVSKLFETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDF 64

Query: 96  KKCTSLTFSCKSWVQPKSSTHQRRIFFSSNK--SYLSGKTPEGLVKLRAEDLANLRGEKE 155
           KKCT LTFSCKSWVQPKSSTHQRRIFFSSNK  SYLSGKTPEGLVKLRAEDLANLRGEKE
Sbjct: 65  KKCTLLTFSCKSWVQPKSSTHQRRIFFSSNKVMSYLSGKTPEGLVKLRAEDLANLRGEKE 124

Query: 156 DGSVDKKKRNAFERIYDYDLYNDLGKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDND 215
           DGSVDKKKRNAFERIYDYDLYNDLGKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDND
Sbjct: 125 DGSVDKKKRNAFERIYDYDLYNDLGKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDND 184

Query: 216 KHAEERIEAPFYVPRDENFSEEKQGSVLVENEALLGKAPFSDLPELDIKTPFAASKINQL 275
           KHAEERIEAPFYVPRDENFSEEKQGSVL ENEALLGKAPFSDLPELDIKTPFAASKINQL
Sbjct: 185 KHAEERIEAPFYVPRDENFSEEKQGSVLAENEALLGKAPFSDLPELDIKTPFAASKINQL 244

Query: 276 PFDIPSLVSSNQPPPLNSLDDEPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYS 335
           PFDIPSLVSSNQPPPLNSLDDEPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYS
Sbjct: 245 PFDIPSLVSSNQPPPLNSLDDEPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYS 304

Query: 336 IQLVKSLPFMSKLDEGVYGPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMP 395
           IQLVKSLPFMSKLDEGVYGPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMP
Sbjct: 305 IQLVKSLPFMSKLDEGVYGPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMP 364

Query: 396 YVSKVRKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDV 455
           YVSKVRKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDV
Sbjct: 365 YVSKVRKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDV 424

Query: 456 WIWRLAKAHVLSHDSCIHQLVIHW 477
           W+WRLAKAHVLSHDSCIHQLVIHW
Sbjct: 425 WLWRLAKAHVLSHDSCIHQLVIHW 448

BLAST of CsGy4G013043 vs. NCBI nr
Match: TYK21645.1 (lipoxygenase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 660 bits (1702), Expect = 3.45e-233
Identity = 356/522 (68.20%), Postives = 399/522 (76.44%), Query Frame = 0

Query: 1   MSYKGTVTVTVNPKKNEAFLTTEVIYLEFVSVELDSKG-NQQIIGCTAGLKCGGDVS--K 60
           MSY G V VTV PKK E F   E+IYL+F SVELDS G +++ I C A L+   +    K
Sbjct: 1   MSYTGNVVVTVKPKKKEEFPWVEIIYLKFASVELDSNGQSKEFIKCEAQLQEVDESVEYK 60

Query: 61  LFETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCT-SLTFSCKSWVQPKSSTH 120
            ++  I V   FGEIGAVIVEL + V ERFIDTI+++ ++   S+TFSCKSWVQPK    
Sbjct: 61  KYKGEIKVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGLIA 120

Query: 121 QRRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYND 180
            RRIFFSSNKSYLSGKTP GLVKLRAEDLANLRGEK +GSVD+ +R AFERIYDYDLYND
Sbjct: 121 HRRIFFSSNKSYLSGKTPGGLVKLRAEDLANLRGEKANGSVDRNERKAFERIYDYDLYND 180

Query: 181 LGKPDESSDLIRPVLGGSEE-YPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEE 240
           LG PD+S DL RPVLGGSE+ YPYPRRCRTGR  S ND  +E+RIE  FYVPRDE FSE 
Sbjct: 181 LGDPDQSMDLKRPVLGGSEDQYPYPRRCRTGRPPSQNDPASEKRIEEWFYVPRDEEFSEI 240

Query: 241 KQGSVLVE-NEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDD 300
           KQ S   + N+ LLGK PFSDLPELDI+TPFAASKIN L F+I SLVSS++PP L+S D 
Sbjct: 241 KQSSSQPDGNKKLLGKVPFSDLPELDIETPFAASKIN-LQFNISSLVSSHRPPALSSSDV 300

Query: 301 EPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPR 360
             S+ VELP PESYKRD+YNWLSDIEFARLTLAGLNPYSI+LV+ LPF S L+E  YGPR
Sbjct: 301 PSSTLVELPPPESYKRDQYNWLSDIEFARLTLAGLNPYSIELVRDLPFKSTLEESDYGPR 360

Query: 361 ESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFF 420
           +SKFT E VQELLGCRI V KAL DKRLFVLDYHD L+PYV KVRKI+ TTLYGSRTLFF
Sbjct: 361 QSKFTPERVQELLGCRITVAKALVDKRLFVLDYHDTLLPYVRKVRKIKGTTLYGSRTLFF 420

Query: 421 LNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIHQLV 480
           LNSD TL PLGIELTRPP+  H EQWKEVFTPGT+STDVW+WRLAKAHVLSHDSCIHQL 
Sbjct: 421 LNSDGTLMPLGIELTRPPMDEHLEQWKEVFTPGTDSTDVWLWRLAKAHVLSHDSCIHQL- 480

Query: 481 IHWYVYSYLYTYIHACIYLLSNMIIVIQAESSLLYGAVCHRH 516
                                       AESSLLYGA+CHR+
Sbjct: 481 ----------------------------AESSLLYGAICHRY 492

BLAST of CsGy4G013043 vs. NCBI nr
Match: XP_004142238.3 (lipoxygenase 2, chloroplastic [Cucumis sativus] >KAE8649494.1 hypothetical protein Csa_017907 [Cucumis sativus])

HSP 1 Score: 649 bits (1675), Expect = 3.59e-224
Identity = 345/486 (70.99%), Postives = 383/486 (78.81%), Query Frame = 0

Query: 1   MSYKGTVTVTVNPKKNEAFLTTEVIYLEFVSVELDSKGN-QQIIGCTAGLKCGGDVSKLF 60
           MSY GTV VT+ PKK+E F   E+IYL+F SVELDS    ++ I C A L+   +V +L 
Sbjct: 1   MSYTGTVVVTMKPKKDEQFRWVEIIYLKFASVELDSNEQPKEFIKCEAQLQ---EVDELV 60

Query: 61  ETS-----IDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCT-SLTFSCKSWVQPKS 120
           E       I V   FGEIGAVIVEL + V ERFIDTI+++ ++   S+TFSCKSWVQPK 
Sbjct: 61  EYKKYIGEIVVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISITFSCKSWVQPKG 120

Query: 121 STHQRRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDL 180
               RRIFFSSNKSYL GKTP GLVKLR EDLANLRGEKEDGSVD+ +R AFERIYDYDL
Sbjct: 121 LIDHRRIFFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDL 180

Query: 181 YNDLGKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFS 240
           YNDLG PD S DL RPVLGGSEEYPYPRRCRTGR  + ND  +E+R+E  FY+PRDE FS
Sbjct: 181 YNDLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFS 240

Query: 241 EEKQGSVLVENEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPP--LNS 300
           E KQ S    N+ LL KAP SDLPE+ IK P AASKIN L  ++ S+V S+QPP   +NS
Sbjct: 241 EIKQSSNQPRNKKLLKKAPSSDLPEIGIKMPSAASKIN-LQCNVSSIVCSHQPPAAAINS 300

Query: 301 LDDEPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVY 360
            D   S+ VELP PESYKRD+YNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEG Y
Sbjct: 301 SDVPSSTLVELPPPESYKRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDY 360

Query: 361 GPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRT 420
           GPRESKFT E VQELLGC I V KAL DKRLFV+DYHD LMPYV +VRKI+ TTLYGSRT
Sbjct: 361 GPRESKFTPERVQELLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSRT 420

Query: 421 LFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIH 477
           LFFLNSD TL PLGIELTRPP+  H EQWKEVFTPGTNSTD+W+WR AKAHVLSHDSCIH
Sbjct: 421 LFFLNSDGTLMPLGIELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCIH 480

BLAST of CsGy4G013043 vs. NCBI nr
Match: KAA0041373.1 (lipoxygenase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 648 bits (1671), Expect = 9.81e-224
Identity = 345/479 (72.03%), Postives = 383/479 (79.96%), Query Frame = 0

Query: 1   MSYKGTVTVTVNPKKNEAFLTTEVIYLEFVSVELDSKGN-QQIIGCTAGLKCGGDVS--K 60
           MSY G V VTV PKK E F   E+IYL+F SVELDS G  ++ I C A L+   +    K
Sbjct: 1   MSYMGNVVVTVKPKKKEEFPWVEIIYLKFASVELDSNGQPKEFIKCEAQLQEVDESVEYK 60

Query: 61  LFETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCT-SLTFSCKSWVQPKSSTH 120
            ++  I V   FGEIGAVIVEL + V ERFIDTI++  ++   S+TFSCKSWVQPK    
Sbjct: 61  KYKGEIKVAEGFGEIGAVIVELQEGVNERFIDTISVVAEEPPISVTFSCKSWVQPKGLIA 120

Query: 121 QRRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYND 180
            RRIFFSSNKSYLSGKTP GLVKLRAEDLANLRGEK +GSVD+ +R AFERIYDYDLYND
Sbjct: 121 HRRIFFSSNKSYLSGKTPGGLVKLRAEDLANLRGEKANGSVDRNERKAFERIYDYDLYND 180

Query: 181 LGKPDESSDLIRPVLGGSEE-YPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEE 240
           LG PD+S DL RPVLGGSE+ YPYPRRCRTGR  S ND  +E+RIE  FYVPRDE FSE 
Sbjct: 181 LGDPDQSMDLKRPVLGGSEDQYPYPRRCRTGRPPSQNDPASEKRIEEWFYVPRDEEFSEI 240

Query: 241 KQGSVLVE-NEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDD 300
           KQ S   + N+ LLGK PFSDLPELDI+TPFAASKIN L F+I SLVSS++PP L+S D 
Sbjct: 241 KQSSSQPDGNKKLLGKVPFSDLPELDIETPFAASKIN-LQFNISSLVSSHRPPALSSSDV 300

Query: 301 EPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPR 360
             S+ VELP PESYKRD+YNWLSDIEFARLTLAGLNPYSIQLV+ LPF S L+E  YGPR
Sbjct: 301 PSSTLVELPPPESYKRDQYNWLSDIEFARLTLAGLNPYSIQLVRDLPFKSTLEESDYGPR 360

Query: 361 ESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFF 420
           +SKFT E V ELLGCRI V KAL DKRLFVLDYHD L+PYV KVRKI+ TTLYGSRTLFF
Sbjct: 361 QSKFTPERVHELLGCRITVAKALVDKRLFVLDYHDTLLPYVRKVRKIKGTTLYGSRTLFF 420

Query: 421 LNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIHQL 473
           LNSD TL PLGIELTRPP+ GH EQWKEVFTPGT+STDVW+WRLAKAHVLSHDSCIHQL
Sbjct: 421 LNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTDSTDVWLWRLAKAHVLSHDSCIHQL 478

BLAST of CsGy4G013043 vs. ExPASy TrEMBL
Match: A0A5D3DDQ4 (Lipoxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00070 PE=3 SV=1)

HSP 1 Score: 660 bits (1702), Expect = 1.67e-233
Identity = 356/522 (68.20%), Postives = 399/522 (76.44%), Query Frame = 0

Query: 1   MSYKGTVTVTVNPKKNEAFLTTEVIYLEFVSVELDSKG-NQQIIGCTAGLKCGGDVS--K 60
           MSY G V VTV PKK E F   E+IYL+F SVELDS G +++ I C A L+   +    K
Sbjct: 1   MSYTGNVVVTVKPKKKEEFPWVEIIYLKFASVELDSNGQSKEFIKCEAQLQEVDESVEYK 60

Query: 61  LFETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCT-SLTFSCKSWVQPKSSTH 120
            ++  I V   FGEIGAVIVEL + V ERFIDTI+++ ++   S+TFSCKSWVQPK    
Sbjct: 61  KYKGEIKVAEGFGEIGAVIVELQEGVNERFIDTISVEAEEPPISVTFSCKSWVQPKGLIA 120

Query: 121 QRRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYND 180
            RRIFFSSNKSYLSGKTP GLVKLRAEDLANLRGEK +GSVD+ +R AFERIYDYDLYND
Sbjct: 121 HRRIFFSSNKSYLSGKTPGGLVKLRAEDLANLRGEKANGSVDRNERKAFERIYDYDLYND 180

Query: 181 LGKPDESSDLIRPVLGGSEE-YPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEE 240
           LG PD+S DL RPVLGGSE+ YPYPRRCRTGR  S ND  +E+RIE  FYVPRDE FSE 
Sbjct: 181 LGDPDQSMDLKRPVLGGSEDQYPYPRRCRTGRPPSQNDPASEKRIEEWFYVPRDEEFSEI 240

Query: 241 KQGSVLVE-NEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDD 300
           KQ S   + N+ LLGK PFSDLPELDI+TPFAASKIN L F+I SLVSS++PP L+S D 
Sbjct: 241 KQSSSQPDGNKKLLGKVPFSDLPELDIETPFAASKIN-LQFNISSLVSSHRPPALSSSDV 300

Query: 301 EPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPR 360
             S+ VELP PESYKRD+YNWLSDIEFARLTLAGLNPYSI+LV+ LPF S L+E  YGPR
Sbjct: 301 PSSTLVELPPPESYKRDQYNWLSDIEFARLTLAGLNPYSIELVRDLPFKSTLEESDYGPR 360

Query: 361 ESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFF 420
           +SKFT E VQELLGCRI V KAL DKRLFVLDYHD L+PYV KVRKI+ TTLYGSRTLFF
Sbjct: 361 QSKFTPERVQELLGCRITVAKALVDKRLFVLDYHDTLLPYVRKVRKIKGTTLYGSRTLFF 420

Query: 421 LNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIHQLV 480
           LNSD TL PLGIELTRPP+  H EQWKEVFTPGT+STDVW+WRLAKAHVLSHDSCIHQL 
Sbjct: 421 LNSDGTLMPLGIELTRPPMDEHLEQWKEVFTPGTDSTDVWLWRLAKAHVLSHDSCIHQL- 480

Query: 481 IHWYVYSYLYTYIHACIYLLSNMIIVIQAESSLLYGAVCHRH 516
                                       AESSLLYGA+CHR+
Sbjct: 481 ----------------------------AESSLLYGAICHRY 492

BLAST of CsGy4G013043 vs. ExPASy TrEMBL
Match: A0A5A7TDY8 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00180 PE=3 SV=1)

HSP 1 Score: 648 bits (1671), Expect = 4.75e-224
Identity = 345/479 (72.03%), Postives = 383/479 (79.96%), Query Frame = 0

Query: 1   MSYKGTVTVTVNPKKNEAFLTTEVIYLEFVSVELDSKGN-QQIIGCTAGLKCGGDVS--K 60
           MSY G V VTV PKK E F   E+IYL+F SVELDS G  ++ I C A L+   +    K
Sbjct: 1   MSYMGNVVVTVKPKKKEEFPWVEIIYLKFASVELDSNGQPKEFIKCEAQLQEVDESVEYK 60

Query: 61  LFETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCT-SLTFSCKSWVQPKSSTH 120
            ++  I V   FGEIGAVIVEL + V ERFIDTI++  ++   S+TFSCKSWVQPK    
Sbjct: 61  KYKGEIKVAEGFGEIGAVIVELQEGVNERFIDTISVVAEEPPISVTFSCKSWVQPKGLIA 120

Query: 121 QRRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYND 180
            RRIFFSSNKSYLSGKTP GLVKLRAEDLANLRGEK +GSVD+ +R AFERIYDYDLYND
Sbjct: 121 HRRIFFSSNKSYLSGKTPGGLVKLRAEDLANLRGEKANGSVDRNERKAFERIYDYDLYND 180

Query: 181 LGKPDESSDLIRPVLGGSEE-YPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEE 240
           LG PD+S DL RPVLGGSE+ YPYPRRCRTGR  S ND  +E+RIE  FYVPRDE FSE 
Sbjct: 181 LGDPDQSMDLKRPVLGGSEDQYPYPRRCRTGRPPSQNDPASEKRIEEWFYVPRDEEFSEI 240

Query: 241 KQGSVLVE-NEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDD 300
           KQ S   + N+ LLGK PFSDLPELDI+TPFAASKIN L F+I SLVSS++PP L+S D 
Sbjct: 241 KQSSSQPDGNKKLLGKVPFSDLPELDIETPFAASKIN-LQFNISSLVSSHRPPALSSSDV 300

Query: 301 EPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPR 360
             S+ VELP PESYKRD+YNWLSDIEFARLTLAGLNPYSIQLV+ LPF S L+E  YGPR
Sbjct: 301 PSSTLVELPPPESYKRDQYNWLSDIEFARLTLAGLNPYSIQLVRDLPFKSTLEESDYGPR 360

Query: 361 ESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFF 420
           +SKFT E V ELLGCRI V KAL DKRLFVLDYHD L+PYV KVRKI+ TTLYGSRTLFF
Sbjct: 361 QSKFTPERVHELLGCRITVAKALVDKRLFVLDYHDTLLPYVRKVRKIKGTTLYGSRTLFF 420

Query: 421 LNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIHQL 473
           LNSD TL PLGIELTRPP+ GH EQWKEVFTPGT+STDVW+WRLAKAHVLSHDSCIHQL
Sbjct: 421 LNSDGTLMPLGIELTRPPMDGHLEQWKEVFTPGTDSTDVWLWRLAKAHVLSHDSCIHQL 478

BLAST of CsGy4G013043 vs. ExPASy TrEMBL
Match: A0A5A7TI85 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00160 PE=3 SV=1)

HSP 1 Score: 632 bits (1631), Expect = 5.65e-218
Identity = 346/483 (71.64%), Postives = 383/483 (79.30%), Query Frame = 0

Query: 1   MSYKGTVTVTVNPKKNEAFLTTEVIYLEFVSVELDSKGNQQIIGCTAGLKCGGD-VSKLF 60
           MSY  TV VTV PK  + F + EVI LEF SV LDS    + I C A LK G + VSKLF
Sbjct: 1   MSYTCTVAVTVKPKSKDDFPSNEVINLEFASVNLDSNEQPKFITCIAQLKSGNNKVSKLF 60

Query: 61  ETSIDVPGDFGEIGAVIVELDDDVK-ERFIDTITIDFKKCT-SLTFSCKSWVQPKSSTHQ 120
           E SI+V  DFGEIGAVIVEL +  K ERFIDTI+++ ++   S+TFSCKSWVQPK     
Sbjct: 61  EGSIEVARDFGEIGAVIVELHERTKNERFIDTISVEAEEPPISVTFSCKSWVQPKGLIAH 120

Query: 121 RRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDG---SVDKKKRNAFERIYDYDLY 180
           RRIFFSSNKSYL  KTP+GLVKLRAEDLANLRGEK      SVD+K+R AFERIYDYD+Y
Sbjct: 121 RRIFFSSNKSYLPEKTPKGLVKLRAEDLANLRGEKLSNGSISVDRKERKAFERIYDYDVY 180

Query: 181 NDLGKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSE 240
           NDLGKPD+S DL RPVLGG+E YPYPRRCRTGR  SDND  +E+R EA FYVPRDE FSE
Sbjct: 181 NDLGKPDQSMDLKRPVLGGTE-YPYPRRCRTGRPPSDNDPASEKRFEAWFYVPRDEEFSE 240

Query: 241 EKQGSV-LVENEALLGKAPFSDLPELDIKTPFAASKINQLPFDIPSLVSSNQPPPLNSLD 300
            KQ S      E LLG++ FSDLP++DI+TP AASKIN LPF+I SLVSS+Q PPLNSLD
Sbjct: 241 IKQKSFDQPGKEKLLGQSSFSDLPQIDIETPSAASKIN-LPFEISSLVSSHQSPPLNSLD 300

Query: 301 DEPSSSVELPLP---ESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGV 360
           DE SSS  +PLP   ES+KRD YNWLSDIEFARLTLAGLNPYSIQLV++LPF SKLD   
Sbjct: 301 DE-SSSFLVPLPPRPESHKRDPYNWLSDIEFARLTLAGLNPYSIQLVRNLPFKSKLDVTK 360

Query: 361 YGPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSR 420
           YGP +SKFT   VQELLGCR+ V  AL  KRLFVLDYHD LMPYV KVR+IE  TLYGSR
Sbjct: 361 YGPPDSKFTLTRVQELLGCRMNVADALKKKRLFVLDYHDTLMPYVQKVREIEDKTLYGSR 420

Query: 421 TLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCI 473
           TLFFLNSDDTL PLGIELTRPPIKG  +QWKE+F PGTNSTDVW+WRLAKAHVLSHDSCI
Sbjct: 421 TLFFLNSDDTLVPLGIELTRPPIKGDPKQWKEIFPPGTNSTDVWLWRLAKAHVLSHDSCI 480

BLAST of CsGy4G013043 vs. ExPASy TrEMBL
Match: A0A6J1FXU8 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111448228 PE=3 SV=1)

HSP 1 Score: 520 bits (1340), Expect = 1.58e-176
Identity = 284/483 (58.80%), Postives = 345/483 (71.43%), Query Frame = 0

Query: 9   VTVNPKKNEAFLTTEVIYLEFVSVELDSKGNQQ-IIGCTAGLKCGGDVSKLFETSIDVPG 68
           VTV PK NE F + + + L+F S +LD+ G Q+ +IG  A ++        +   I VP 
Sbjct: 22  VTVRPKFNEKFPSDDTLSLKFASSDLDADGQQKPLIGGVATIQNESAEEYTYVAKIQVPR 81

Query: 69  DFGEIGAVIVELDDDVKERFIDTITI-DFKKCTSLTFSCKSWVQPKSST-HQRRIFFSSN 128
            FGEIGAVI+EL D+  ERFIDT+++ +     S+TFSC SWVQPK+    QRRIFFS+ 
Sbjct: 82  GFGEIGAVIIELKDNSTERFIDTVSVANSSSQNSVTFSCTSWVQPKNLIPDQRRIFFST- 141

Query: 129 KSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLGKPDESSD 188
           KSYL G TP GLVKLRAEDLANLRG+K DG+ D+ +R AFERIYDYD+YNDLG PD    
Sbjct: 142 KSYLPGNTPAGLVKLRAEDLANLRGQKPDGTEDENERKAFERIYDYDVYNDLGDPDVDMK 201

Query: 189 LIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQGSVLVE-- 248
             RPVLGGS +YPYPRRCRTGR  + +D  +E R E  FYVPRDE FSE KQ        
Sbjct: 202 WKRPVLGGSAQYPYPRRCRTGRPAAVSDPWSEHRGEESFYVPRDEAFSEVKQKMFPANPG 261

Query: 249 NEALLGKAPFSDLPELD------IKTPFAASKINQLPFDIPSLVSSNQPPPL---NSLDD 308
            +  LG  PFSD  ++D      I+ P A  +I  L F+I S +S+   PP    ++L  
Sbjct: 262 KKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEI--LRFNISSTLSTMHAPPFVDPSTLQP 321

Query: 309 EPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPR 368
           +P+  V+ P PE+ KRDK+NWLSD EFAR TLAGLNPYSIQLVKSLP MS+L+   YGP+
Sbjct: 322 QPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQ 381

Query: 369 ESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFF 428
           ES FT ELVQ+L+GC I V +AL  K+LFVLDYHD LM YV+KVR I+ TTLYGSRTLFF
Sbjct: 382 ESGFTTELVQKLIGCSITVEEALAHKKLFVLDYHDTLMQYVTKVRSIKRTTLYGSRTLFF 441

Query: 429 LNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIHQLV 477
           LNSDDTL PLGIELTRPP+ G   QWK+VF+P T++TD W+WRLAKAHVL+HDSC+HQLV
Sbjct: 442 LNSDDTLTPLGIELTRPPMDG-KPQWKQVFSPSTHATDAWLWRLAKAHVLAHDSCVHQLV 500

BLAST of CsGy4G013043 vs. ExPASy TrEMBL
Match: A0A6J1FUE7 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111448228 PE=3 SV=1)

HSP 1 Score: 520 bits (1340), Expect = 9.53e-174
Identity = 284/483 (58.80%), Postives = 345/483 (71.43%), Query Frame = 0

Query: 9   VTVNPKKNEAFLTTEVIYLEFVSVELDSKGNQQ-IIGCTAGLKCGGDVSKLFETSIDVPG 68
           VTV PK NE F + + + L+F S +LD+ G Q+ +IG  A ++        +   I VP 
Sbjct: 22  VTVRPKFNEKFPSDDTLSLKFASSDLDADGQQKPLIGGVATIQNESAEEYTYVAKIQVPR 81

Query: 69  DFGEIGAVIVELDDDVKERFIDTITI-DFKKCTSLTFSCKSWVQPKSST-HQRRIFFSSN 128
            FGEIGAVI+EL D+  ERFIDT+++ +     S+TFSC SWVQPK+    QRRIFFS+ 
Sbjct: 82  GFGEIGAVIIELKDNSTERFIDTVSVANSSSQNSVTFSCTSWVQPKNLIPDQRRIFFST- 141

Query: 129 KSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLGKPDESSD 188
           KSYL G TP GLVKLRAEDLANLRG+K DG+ D+ +R AFERIYDYD+YNDLG PD    
Sbjct: 142 KSYLPGNTPAGLVKLRAEDLANLRGQKPDGTEDENERKAFERIYDYDVYNDLGDPDVDMK 201

Query: 189 LIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQGSVLVE-- 248
             RPVLGGS +YPYPRRCRTGR  + +D  +E R E  FYVPRDE FSE KQ        
Sbjct: 202 WKRPVLGGSAQYPYPRRCRTGRPAAVSDPWSEHRGEESFYVPRDEAFSEVKQKMFPANPG 261

Query: 249 NEALLGKAPFSDLPELD------IKTPFAASKINQLPFDIPSLVSSNQPPPL---NSLDD 308
            +  LG  PFSD  ++D      I+ P A  +I  L F+I S +S+   PP    ++L  
Sbjct: 262 KKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEI--LRFNISSTLSTMHAPPFVDPSTLQP 321

Query: 309 EPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPR 368
           +P+  V+ P PE+ KRDK+NWLSD EFAR TLAGLNPYSIQLVKSLP MS+L+   YGP+
Sbjct: 322 QPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQ 381

Query: 369 ESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFF 428
           ES FT ELVQ+L+GC I V +AL  K+LFVLDYHD LM YV+KVR I+ TTLYGSRTLFF
Sbjct: 382 ESGFTTELVQKLIGCSITVEEALAHKKLFVLDYHDTLMQYVTKVRSIKRTTLYGSRTLFF 441

Query: 429 LNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIHQLV 477
           LNSDDTL PLGIELTRPP+ G   QWK+VF+P T++TD W+WRLAKAHVL+HDSC+HQLV
Sbjct: 442 LNSDDTLTPLGIELTRPPMDG-KPQWKQVFSPSTHATDAWLWRLAKAHVLAHDSCVHQLV 500

BLAST of CsGy4G013043 vs. TAIR 10
Match: AT3G45140.1 (lipoxygenase 2 )

HSP 1 Score: 340.1 bits (871), Expect = 3.1e-93
Identity = 196/439 (44.65%), Postives = 264/439 (60.14%), Query Frame = 0

Query: 59  FETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCTSLTFSCKSWVQPKSSTHQR 118
           +E   ++P DFG +GA+ ++ +   ++ F+  + +      S+TF+C+SWV PKS    +
Sbjct: 137 YECEFEMPEDFGPVGAIKIQ-NQYHRQLFLKGVELKLPG-GSITFTCESWVAPKSVDPTK 196

Query: 119 RIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLG 178
           RIFF S+KSYL  +TPE L K R E+L  L+G+  +   +  K   FERIYDYD+YND+G
Sbjct: 197 RIFF-SDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEFTK---FERIYDYDVYNDVG 256

Query: 179 KPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIEAPFYVPRDENFSEEKQG 238
            PD   +L RPV+GG   +PYPRRC+TGR   + D  +E+R    FYVPRDE FS  K  
Sbjct: 257 DPDNDPELARPVIGGL-THPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEFSTAK-- 316

Query: 239 SVLVENEALLGKAPFSDLPE---------LDIKTPFAASKI--------NQLPFD---IP 298
                  +  GKA  + LP          L  + PF   K          QLP D   +P
Sbjct: 317 -----GTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKDAGLLP 376

Query: 299 SLVSSNQPPPLNSLDDEPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVK 358
            L     P  + +L +     ++   P    RD+++WL D EFAR TLAGLNPYSIQLV+
Sbjct: 377 LL-----PRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVE 436

Query: 359 SLPFMSKLDEGVYGPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKV 418
             P +SKLD  VYG   S  T E+V+  +   + V +AL +KRLFVLDYHD+L+PYV+KV
Sbjct: 437 EWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKV 496

Query: 419 RKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRL 478
           R++  TTLY SRTLFFL+ D TL P+ IELT PP   +  QWK+VFTPG ++T  W+W L
Sbjct: 497 RELNNTTLYASRTLFFLSDDSTLRPVAIELTCPP-NINKPQWKQVFTPGYDATSCWLWNL 555

BLAST of CsGy4G013043 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-80
Identity = 198/533 (37.15%), Postives = 283/533 (53.10%), Query Frame = 0

Query: 1   MSYKGTVTVTVNPKKNEAFLTTEV-------------IYLEFVSVELDSKGNQQIIGCTA 60
           + +K    VTV  K  E    T V             I LE +S +LD K         A
Sbjct: 82  VKFKVRAVVTVRNKNKEDLKETLVKHLDAFADKIGRNIVLELISTQLDPKTKLPKKSNAA 141

Query: 61  GLKCGGDVSKL------FETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCTSL 120
            LK     SK       +     V   FG  GA+ V ++   KE F+++ITI+      +
Sbjct: 142 VLKDWSKKSKTKAERVHYTAEFTVDAAFGSPGAITV-MNKHQKEFFLESITIEGFALGPV 201

Query: 121 TFSCKSWVQPKSSTHQRRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKK 180
            F C SWVQ +     +RIFF +N+ YL  +TP GL  LR ++L NLRG   DGS     
Sbjct: 202 HFPCNSWVQSQKDHPDKRIFF-TNQPYLPNETPSGLRVLREKELKNLRG---DGS---GV 261

Query: 181 RNAFERIYDYDLYNDLGKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIE 240
           R   +RIYD+D+YNDLG PD+SS+L RP LGG +E PYPRRCRTGR ++ +DK AE R+E
Sbjct: 262 RKLSDRIYDFDVYNDLGNPDKSSELSRPKLGG-KEVPYPRRCRTGRQSTVSDKDAESRVE 321

Query: 241 --APFYVPRDENFSEEKQGS------------VLVENEALLGKAPFSDLPELD------- 300
              P YVPRDE F E KQ +            ++   +A +    F+D  E+D       
Sbjct: 322 KPLPMYVPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKASIVAEDFADFGEIDRLYKEGL 381

Query: 301 -IKTPFAASKINQLPFDIPSLVSSNQPPPLNSLDDEPSSSVELPLPESYKRDKYNWLSDI 360
            +K  F      + P  +P +V       +++L +     ++   P+   +DK  WL D 
Sbjct: 382 LLKLGFQDDIFKKFP--LPKVV-------VDTLQESTKGLLKYDTPKILSKDKNAWLRDD 441

Query: 361 EFARLTLAGLNPYSIQLVKSLPFMSKLDEGVYGPRESKFTHE-LVQELLGCRIKVCKALT 420
           EFAR  +AG+NP +I+ VK+ P +S LD  +YGP+ S  T + ++  L G    V +AL 
Sbjct: 442 EFARQAIAGINPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIGHLDG--FSVQQALE 501

Query: 421 DKRLFVLDYHDILMPYVSKVRKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHSE 480
           + RL++LDYHDI +P++ ++  ++    Y +RT+FFL    TL+P+ IEL+ PP  G   
Sbjct: 502 ENRLYMLDYHDIFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIELSLPP-HGPKH 561

Query: 481 QWKEVFTPGTNSTDVWIWRLAKAHVLSHDSCIHQLVIHWYVYSYLYTYIHACI 492
           + K V TP  ++T  W+W+LAKAHV S+D+ +HQLV HW          HAC+
Sbjct: 562 RSKRVLTPPVDATSNWMWQLAKAHVSSNDAGVHQLVNHW-------LRTHACL 586

BLAST of CsGy4G013043 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.8e-77
Identity = 187/526 (35.55%), Postives = 273/526 (51.90%), Query Frame = 0

Query: 1   MSYKGTVTVTVNPKKNEAFLTTEV-------------IYLEFVSVELDSKGNQQIIGCTA 60
           + +K     TV  K  E F  T V             + LE +S ++D K N+      A
Sbjct: 88  VKFKVRAVATVRNKNKEDFKETLVKHLDAFTDKIGRNVVLELMSTQVDPKTNEPKKSKAA 147

Query: 61  GLKCGGDVSKL------FETSIDVPGDFGEIGAVIVELDDDVKERFIDTITIDFKKCTSL 120
            LK     S        +     V   FG  GA+ V  +   KE F+++ITI+   C  +
Sbjct: 148 VLKDWSKKSNSKAERVHYTAEFTVDSAFGSPGAITV-TNKHQKEFFLESITIEGFACGPV 207

Query: 121 TFSCKSWVQPKSSTHQRRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKK 180
            F C SWVQ +     +RI F +N+ YL  +TP GL  LR ++L NLRG        K +
Sbjct: 208 HFPCNSWVQSQKDHPSKRILF-TNQPYLPSETPSGLRTLREKELENLRGN------GKGE 267

Query: 181 RNAFERIYDYDLYNDLGKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERIE 240
           R   +RIYDYD+YND+G PD S +L RP LGG  E+PYPRRCRTGR ++D D  +E R+E
Sbjct: 268 RKLSDRIYDYDVYNDIGNPDISRELARPTLGG-REFPYPRRCRTGRSSTDTDMMSERRVE 327

Query: 241 --APFYVPRDENFSEEKQG------------SVLVENEALLGKAPFSDLPELDIKTPFAA 300
              P YVPRDE F E KQ             +++   +A +    F++  E+D  + +  
Sbjct: 328 KPLPMYVPRDEQFEESKQNTFAACRLKAVLHNLIPSLKASILAEDFANFGEID--SLYKE 387

Query: 301 SKINQLPFDIPSLVSSNQPPPLNSLDDEPSSSVELPLPESYKRDKYNWLSDIEFARLTLA 360
             + +L F          P  + +L       +    P+   +DKY WL D EFAR  +A
Sbjct: 388 GLLLKLGFQDDMFKKFPLPKIVTTLQKSSEGLLRYDTPKIVSKDKYAWLRDDEFARQAIA 447

Query: 361 GLNPYSIQLVKSLPFMSKLDEGVYGP-RESKFTHE-LVQELLGCRIKVCKALTDKRLFVL 420
           G+NP +I+ V S P +S LD  +YGP   S  T + ++ +L G  + V +AL   RLF++
Sbjct: 448 GINPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHIIGQLDG--LTVQQALETNRLFMV 507

Query: 421 DYHDILMPYVSKVRKIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFT 480
           DYHDI +P++ ++  ++    Y +RT+ FL    TL+P+ IEL+ P     +++ K V T
Sbjct: 508 DYHDIYLPFLDRINALDGRKAYATRTILFLTRLGTLKPIAIELSLPSQSSSNQKSKRVVT 567

Query: 481 PGTNSTDVWIWRLAKAHVLSHDSCIHQLVIHWYVYSYLYTYIHACI 492
           P  ++T  W+W+LAKAHV S+D+ +HQLV HW          HAC+
Sbjct: 568 PPVDATSNWMWQLAKAHVGSNDAGVHQLVNHW-------LRTHACL 593

BLAST of CsGy4G013043 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 256.9 bits (655), Expect = 3.4e-68
Identity = 193/510 (37.84%), Postives = 270/510 (52.94%), Query Frame = 0

Query: 27  LEFVSVELD---SKGNQQIIGCTAGL-KCGGDVSKL-FETSIDVPGDFGEIGAVIVE--- 86
           ++ VS E+D    KG + +     GL K   D   L F     VP +FG+ GA++V    
Sbjct: 117 IQLVSEEIDPETGKGRKSLESPVMGLPKAVKDPRYLVFTADFTVPINFGKPGAILVTNLL 176

Query: 87  -----LDDDVKERFIDTITIDFKKCTSLTFSCKSWVQPKSSTHQRRIFFSSNKSYLSGKT 146
                L + + E   DTI           F   +W+  K+   Q RI F S +  L  +T
Sbjct: 177 STEICLSEIIIEDSTDTI----------LFPANTWIHSKNDNPQARIIFRS-QPCLPSET 236

Query: 147 PEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDLGKPDESSDLIRPVLGG 206
           P+G+ +LR +DL ++RG+       K +R   ERIYDYD+YNDLG P   ++ +RPVL G
Sbjct: 237 PDGIKELREKDLVSVRGD------GKGERKPHERIYDYDVYNDLGDP-RKTERVRPVL-G 296

Query: 207 SEEYPYPRRCRTGRDTSDNDKHAEER--IEAPFYVPRDENFSEEKQGSVLVENEALLGKA 266
             E PYPRRCRTGR     D   E R   +  FYVPRDE F E K+ +      A   KA
Sbjct: 297 VPETPYPRRCRTGRPLVSKDPPCESRGKEKEEFYVPRDEVFEEIKRDTF----RAGRFKA 356

Query: 267 PFSDLPELDIKTPFAASKINQLPF----DIPSLVSSN------QPPP----------LNS 326
            F +L    + +  AA     +PF    DI +L  SN      +P            +N 
Sbjct: 357 LFHNL----VPSIAAALSNLDIPFTCFSDIDNLYKSNIVLGHTEPKDTGLGGFIGGFMNG 416

Query: 327 LDDEPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGVY 386
           + +   + ++   P   K D++ WL D EF R  LAG+NP +I+L+K LP  S LD  +Y
Sbjct: 417 ILNVTETLLKYDTPAVIKWDRFAWLRDNEFGRQALAGVNPVNIELLKELPIRSNLDPALY 476

Query: 387 GPRESKFTHELV-QELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVRKI--EWTTLYG 446
           GP+ES  T E++ +E+      + KAL +KRLF++DYHDIL+P+V K+  I  +    Y 
Sbjct: 477 GPQESVLTEEIIAREVEHYGTTIEKALEEKRLFLVDYHDILLPFVEKINSIKEDPRKTYA 536

Query: 447 SRTLFFLNSDDTLEPLGIELTRPPIKGHSEQWKEVFTPGTNSTDVWIWRLAKAHVLSHDS 498
           SRT+FF + +  L PL IEL+ PP    SE  K V+T G ++T  WIW+LAKAHV S+D+
Sbjct: 537 SRTIFFYSKNGALRPLAIELSLPP-TAESEN-KFVYTHGHDATTHWIWKLAKAHVCSNDA 596

BLAST of CsGy4G013043 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 238.8 bits (608), Expect = 9.7e-63
Identity = 167/439 (38.04%), Postives = 230/439 (52.39%), Query Frame = 0

Query: 59  FETSIDVPGDFGEIGAVIVELDDDVKERFIDTITI-DFKKCTSLTFSCKSWVQPKSSTHQ 118
           F+ + D   DFG  GA ++  +    E  + ++T+ D      + + C SW+ P      
Sbjct: 97  FKVTFDYETDFGYPGAFLIR-NSHFSEFLLKSLTLEDVPGHGRVHYICNSWIYPAKHYTT 156

Query: 119 RRIFFSSNKSYLSGKTPEGLVKLRAEDLANLRGEKEDGSVDKKKRNAFERIYDYDLYNDL 178
            R+FF SNK+YL  +TP  L+K R E+L +LRG  E       +   ++R+YDY  YNDL
Sbjct: 157 DRVFF-SNKTYLPHETPATLLKYREEELVSLRGTGEG------ELKEWDRVYDYAYYNDL 216

Query: 179 GKPDESSDLIRPVLGGSEEYPYPRRCRTGRDTSDNDKHAEERI----EAPFYVPRDENFS 238
           G P ++    RPVLGG++EYPYPRR RTGR  +  D   E R+        YVPRDE F 
Sbjct: 217 GVPPKNP---RPVLGGTQEYPYPRRGRTGRKPTKEDPQTESRLPITSSLDIYVPRDERFG 276

Query: 239 EEKQGSVLVENEALLG-------KAPFSDLPELDIKTPFAASKINQLPFDIPS---LVSS 298
             K    L      +        +A F D P+ +  +     KI +   D+P+   + S 
Sbjct: 277 HLKMSDFLAYALKAIAQFIQPALEAVFDDTPK-EFDSFEDVLKIYEEGIDLPNQALIDSI 336

Query: 299 NQPPPLNSLDD----EPSSSVELPLPESYKRDKYNWLSDIEFARLTLAGLNPYSIQLVKS 358
            +  PL  L +    +    ++ P+P+  K DK  W +D EFAR  LAGLNP  IQL+K 
Sbjct: 337 VKNIPLEMLKEIFRTDGQKFLKFPVPQVIKEDKTAWRTDEEFAREMLAGLNPVVIQLLKE 396

Query: 359 LPFMSKLDEGVYGPRESKFTHELVQELLGCRIKVCKALTDKRLFVLDYHDILMPYVSKVR 418
            P  SKLD   YG + S  T   ++  L   + V +AL  +RLF+LD+HD LMPY+ +V 
Sbjct: 397 FPPKSKLDSESYGNQNSTITKSHIEHNLD-GLTVEEALEKERLFILDHHDTLMPYLGRVN 456

Query: 419 KIEWTTLYGSRTLFFLNSDDTLEPLGIELTRPPIKGHS-EQWKEVFTPGTNSTDVWIWRL 478
               T  Y SRTL FL  D TL+PL IEL+ P   G       EV+TPG    D  +W+L
Sbjct: 457 TTT-TKTYASRTLLFLKDDGTLKPLVIELSLPHPNGDKFGAVSEVYTPGEGVYDS-LWQL 516

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
R9WS045.1e-10144.60Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=... [more]
O243707.2e-9543.91Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO... [more]
P384193.6e-9442.00Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX... [more]
Q84YK81.0e-9342.18Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 ... [more]
P384184.3e-9244.65Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAE8649495.10.098.84hypothetical protein Csa_018136 [Cucumis sativus][more]
XP_031740428.10.098.42LOW QUALITY PROTEIN: lipoxygenase 2, chloroplastic-like [Cucumis sativus][more]
TYK21645.13.45e-23368.20lipoxygenase 2 [Cucumis melo var. makuwa][more]
XP_004142238.33.59e-22470.99lipoxygenase 2, chloroplastic [Cucumis sativus] >KAE8649494.1 hypothetical prote... [more]
KAA0041373.19.81e-22472.03lipoxygenase 2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A5D3DDQ41.67e-23368.20Lipoxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00070... [more]
A0A5A7TDY84.75e-22472.03Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00180 P... [more]
A0A5A7TI855.65e-21871.64Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00160 P... [more]
A0A6J1FXU81.58e-17658.80Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111448228 PE=3 SV=1[more]
A0A6J1FUE79.53e-17458.80Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111448228 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G45140.13.1e-9344.65lipoxygenase 2 [more]
AT1G17420.11.3e-8037.15lipoxygenase 3 [more]
AT1G72520.11.8e-7735.55PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G67560.13.4e-6837.84PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G55020.19.7e-6338.04lipoxygenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001246Lipoxygenase, plantPRINTSPR00468PLTLPOXGNASEcoord: 300..316
score: 28.29
coord: 198..217
score: 55.85
coord: 165..181
score: 73.59
coord: 339..358
score: 40.85
coord: 407..431
score: 57.28
coord: 93..111
score: 33.82
IPR001024PLAT/LH2 domainSMARTSM00308LH2_4coord: 3..124
e-value: 1.8E-8
score: 44.2
IPR001024PLAT/LH2 domainPROSITEPS50095PLATcoord: 3..123
score: 16.390484
NoneNo IPR availableGENE3D4.10.375.10coord: 129..235
e-value: 1.7E-36
score: 126.7
NoneNo IPR availableGENE3D2.60.60.20PLAT/LH2 domaincoord: 6..128
e-value: 2.8E-20
score: 74.8
NoneNo IPR availableGENE3D3.10.450.60coord: 333..467
e-value: 6.4E-43
score: 148.0
NoneNo IPR availablePANTHERPTHR11771:SF156LIPOXYGENASEcoord: 17..497
IPR013819Lipoxygenase, C-terminalPFAMPF00305Lipoxygenasecoord: 136..495
e-value: 2.3E-105
score: 353.6
IPR013819Lipoxygenase, C-terminalPROSITEPS51393LIPOXYGENASE_3coord: 127..477
score: 86.052864
IPR027433Lipoxygenase, domain 3GENE3D4.10.372.10coord: 260..331
e-value: 2.0E-8
score: 36.7
IPR000907LipoxygenasePANTHERPTHR11771LIPOXYGENASEcoord: 17..497
IPR036226Lipoxigenase, C-terminal domain superfamilySUPERFAMILY48484Lipoxigenasecoord: 130..478
IPR036392PLAT/LH2 domain superfamilySUPERFAMILY49723Lipase/lipooxygenase domain (PLAT/LH2 domain)coord: 3..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G013043.1CsGy4G013043.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0031408 oxylipin biosynthetic process
biological_process GO:0070816 phosphorylation of RNA polymerase II C-terminal domain
biological_process GO:0006360 transcription by RNA polymerase I
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000439 transcription factor TFIIH core complex
cellular_component GO:0005675 transcription factor TFIIH holo complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function GO:0005515 protein binding
molecular_function GO:0016491 oxidoreductase activity