CsGy4G011410 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G011410
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPectinesterase
LocationGy14Chr4: 11022864 .. 11024681 (-)
RNA-Seq ExpressionCsGy4G011410
SyntenyCsGy4G011410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGAAATGTGAGTTCAAGTAAAATATAAAAGGAGAAAAGATCAAATTGTCAAGTTAAGAAAAAAAATGGGGAAAATATTAAATGAATTTTATGCAAAAAAGCTGAGATTTATTCTGCCTTTCTTCCATAGCGTTTTAAAAAGAGGCAACAAAAACACCACGAAATGGGGGCAATATTTTCAATTAAAAAGAAGATTTAGAGGAGAAAATGGTAAGAAAACATACCTAAACGAAAATAAAAATAAACTGTTGTGAAAAATAACTATTCTTAACACATAATAGTTAATTTATTTTAATAATAATGAAGCAAATTTGATAGGAAAATGCTGTTTAATGACATATTCATTAATTTTGATGATTTAAATCAAGAAAAATGTATCCATTGATTGCATGCATAAATCCTCGATCTAACCTTTTTTTTTCCTTTTAAAGATGTATTTTAGTTTACTACATTAATTAGTATTAAACCAAATGAATTTTTAATGAACCATCCATTCTCTATAAATACAAGGGCTTCCTAGTCCCTATTTTCACATATCCTTTTTGCATTCAATAATATTTTGTCTATTACTTTCAACTTAACAATCATGACAATATTCACACTTGAAATGATCTTTGTACTGTATTTGTTAGTTGTTGTTATTGTTGAGTTTTCTGATTGCTTTAGTTTCAATTCCACAGTGTCTTTAGATGGTACAGAAAATTTTATTAAAATTAACGATGCTATAGCAGCAGCACCCAATTTTAGTACGACAAGATTTTACATTCATGTCAAACCTGGAACTTACCAGGAGATTATTGAAGTTCCATACGAGAAAACTTGTATTGCTTTAATTGGAGATGATTCTTCTACTACCATCATCGTTAACAATCGAAGTAATGGCACTGGGTCCTCCACGGCATCTTCTGCGACTCTCAGTAAGCTTCAACTATCTCAACCCTCATTTTAACAAGGACTTGTTTATAATCATTTTTGTAATTTTTTTTAAAAAATTAGATCTATATATAAACACCATTTTACCTTTAATTTTGTAAGAAGTTGACGAAAAAAATATAGGTTTAATTTTAAAAAATCAAATTATTAGTAAATTTTATAATTTATTACTCAAACCAATGTTTGATTTTGGAAGCTGTAAATGGATCAAACTTCATGGCTCAGTTCTTGACCTTTCAAAACACTGCTGGACCAAATGAAGGCCAAGCAATTGCTCTTCTTGACCAAGCAACACACACAGCTTACTACAAGTGTGTGTTTTTGGGTTATCAAGATACTCTCTATGCTGGAGCACTTCCCCAGTTCTTCAAAGAATGCGATATCTATGGAAGCGTCGACTTCATTTTTGGAAATGGACTTGTTGTATTCCAAGATTGTAATATATACGCTCGATTATTTGACGTTCAAATTACCGTAACAGCACAATCGAAACCAAGTTTAAATTCTCTGAGCGGCTTCATCTTCCAAAACTGCAATGTGACTGTGTCGCCAGAGATAGCACCAAGTAAGGACAACGTAACGGTGTTTCTTGGACGACCATGGAGAATGTACTCAATGGTTGTTTTCATTGATTCATTTCTTGACAACGTGGTTCAACCTAAAGGATGGTTGGAGTGGCCGGGAGTGCCAGAGAATTTATTGTATTATGCAGAATACAATAACAGTGGTGGAGGAGCCAACACTTCTCAAAGGGTTAATTGGCCTGGTTATCATGCGTTAAATAACGCAAAAGAAGTTGCAACTTTTACTGTTGAAACATTTATTAATGGGACTCAATGGTTGCCTCAAACTGGCATACCATTTAGAGCTGGTTTCTAG

mRNA sequence

ATGGAGAAGAAATTGTCTTTAGATGGTACAGAAAATTTTATTAAAATTAACGATGCTATAGCAGCAGCACCCAATTTTAGTACGACAAGATTTTACATTCATGTCAAACCTGGAACTTACCAGGAGATTATTGAAGTTCCATACGAGAAAACTTGTATTGCTTTAATTGGAGATGATTCTTCTACTACCATCATCGTTAACAATCGAAGTAATGGCACTGGGTCCTCCACGGCATCTTCTGCGACTCTCACTGTAAATGGATCAAACTTCATGGCTCAGTTCTTGACCTTTCAAAACACTGCTGGACCAAATGAAGGCCAAGCAATTGCTCTTCTTGACCAAGCAACACACACAGCTTACTACAAGTGTGTGTTTTTGGGTTATCAAGATACTCTCTATGCTGGAGCACTTCCCCAGTTCTTCAAAGAATGCGATATCTATGGAAGCGTCGACTTCATTTTTGGAAATGGACTTGTTGTATTCCAAGATTGTAATATATACGCTCGATTATTTGACGTTCAAATTACCGTAACAGCACAATCGAAACCAAGTTTAAATTCTCTGAGCGGCTTCATCTTCCAAAACTGCAATGTGACTGTGTCGCCAGAGATAGCACCAAGTAAGGACAACGTAACGGTGTTTCTTGGACGACCATGGAGAATGTACTCAATGGTTGTTTTCATTGATTCATTTCTTGACAACGTGGTTCAACCTAAAGGATGGTTGGAGTGGCCGGGAGTGCCAGAGAATTTATTGTATTATGCAGAATACAATAACAGTGGTGGAGGAGCCAACACTTCTCAAAGGGTTAATTGGCCTGGTTATCATGCGTTAAATAACGCAAAAGAAGTTGCAACTTTTACTGTTGAAACATTTATTAATGGGACTCAATGGTTGCCTCAAACTGGCATACCATTTAGAGCTGGTTTCTAG

Coding sequence (CDS)

ATGGAGAAGAAATTGTCTTTAGATGGTACAGAAAATTTTATTAAAATTAACGATGCTATAGCAGCAGCACCCAATTTTAGTACGACAAGATTTTACATTCATGTCAAACCTGGAACTTACCAGGAGATTATTGAAGTTCCATACGAGAAAACTTGTATTGCTTTAATTGGAGATGATTCTTCTACTACCATCATCGTTAACAATCGAAGTAATGGCACTGGGTCCTCCACGGCATCTTCTGCGACTCTCACTGTAAATGGATCAAACTTCATGGCTCAGTTCTTGACCTTTCAAAACACTGCTGGACCAAATGAAGGCCAAGCAATTGCTCTTCTTGACCAAGCAACACACACAGCTTACTACAAGTGTGTGTTTTTGGGTTATCAAGATACTCTCTATGCTGGAGCACTTCCCCAGTTCTTCAAAGAATGCGATATCTATGGAAGCGTCGACTTCATTTTTGGAAATGGACTTGTTGTATTCCAAGATTGTAATATATACGCTCGATTATTTGACGTTCAAATTACCGTAACAGCACAATCGAAACCAAGTTTAAATTCTCTGAGCGGCTTCATCTTCCAAAACTGCAATGTGACTGTGTCGCCAGAGATAGCACCAAGTAAGGACAACGTAACGGTGTTTCTTGGACGACCATGGAGAATGTACTCAATGGTTGTTTTCATTGATTCATTTCTTGACAACGTGGTTCAACCTAAAGGATGGTTGGAGTGGCCGGGAGTGCCAGAGAATTTATTGTATTATGCAGAATACAATAACAGTGGTGGAGGAGCCAACACTTCTCAAAGGGTTAATTGGCCTGGTTATCATGCGTTAAATAACGCAAAAGAAGTTGCAACTTTTACTGTTGAAACATTTATTAATGGGACTCAATGGTTGCCTCAAACTGGCATACCATTTAGAGCTGGTTTCTAG

Protein sequence

MEKKLSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGIPFRAGF*
Homology
BLAST of CsGy4G011410 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.6e-79
Identity = 145/309 (46.93%), Postives = 198/309 (64.08%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           +++DGT NF  INDA+ AAPN S TRF I++K G Y E +E+P +KT I  IGD    T+
Sbjct: 224 VAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTV 283

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           I  NRS   G ST  + T+ V G  ++A+ ++F N+AGP + QA+A    + H+A+Y+C 
Sbjct: 284 IKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCE 343

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDV--QITVTAQSK 184
           F GYQDTLY  +  QF++ECDIYG++DFIFGN  VVFQ+ ++YAR  +   +I  TAQS+
Sbjct: 344 FDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSR 403

Query: 185 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 244
              +  +G    NC +  +P++ P K+N   +LGRPWR YS  V I SF+D+++ P GWL
Sbjct: 404 NQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWL 463

Query: 245 EW-PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQ 304
           E         LYY EY N G GAN ++RV WPG+  + N  E   FTV  FI+G+ WL  
Sbjct: 464 EGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNS 523

Query: 305 TGIPFRAGF 311
           TGIPF  GF
Sbjct: 524 TGIPFSLGF 532

BLAST of CsGy4G011410 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 4.8e-78
Identity = 144/304 (47.37%), Postives = 198/304 (65.13%), Query Frame = 0

Query: 8   DGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTIIVN 67
           DG+ N   I +A+AAA     +R+ I++K GTY E IEV  +   I  +GD    TII  
Sbjct: 208 DGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIGKTIITG 267

Query: 68  NRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLG 127
           ++S G G++T  SAT+ V G NF+A+ +T +NTAGPN  QA+AL   +  + +Y+C F G
Sbjct: 268 SKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEG 327

Query: 128 YQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQI-TVTAQSKPSLN 187
           YQDTLY  +  QF++ECDIYG+VDFIFGN  VV Q+CNI+AR    +  T+TAQ +   N
Sbjct: 328 YQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQGRTDPN 387

Query: 188 SLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPG 247
             +G I  NC VT + ++ P + +V  FLGRPW+ YS  V+I +FLD+++ P GW+EW G
Sbjct: 388 QSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSG 447

Query: 248 -VPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGIP 307
               N LYYAEY N+G G++T+ RV W GYH L +  +V+ FTV  FI G  WLP T +P
Sbjct: 448 DFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVP 507

Query: 308 FRAG 310
           F +G
Sbjct: 508 FTSG 509

BLAST of CsGy4G011410 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 287.0 bits (733), Expect = 2.6e-76
Identity = 140/308 (45.45%), Postives = 197/308 (63.96%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           ++LDGT NF KI DAI  AP++S+TRF I++K G Y E +E+  +K  I ++GD    T+
Sbjct: 215 VALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDGIDVTV 274

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           I  NRS   G +T  SAT  V+G  F+A+ +TFQNTAGP + QA+AL   +  + +++C 
Sbjct: 275 ISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSVFFRCA 334

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYAR--LFDVQITVTAQSK 184
             GYQDTLY   + QF++EC I G+VDFIFG+G VVFQ+C I A+  L + + T+TAQ +
Sbjct: 335 MRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTITAQGR 394

Query: 185 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 244
             +N  SGF  Q  N++   ++ P  +    +LGRPW++YS  VFI + + +VV+P+GWL
Sbjct: 395 KDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWL 454

Query: 245 EW-PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQ 304
           EW      + L+Y E+ N G G+  S RV WPGYH  NN+ +   FTV  FI G  WLP 
Sbjct: 455 EWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKGNLWLPS 514

Query: 305 TGIPFRAG 310
           TG+ F  G
Sbjct: 515 TGVTFSDG 522

BLAST of CsGy4G011410 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.8e-75
Identity = 139/308 (45.13%), Postives = 192/308 (62.34%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           ++ DGT NF  I+DA+ AAP++ST R+ IHVK G Y E +E+  +K  I ++GD    T+
Sbjct: 217 VAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATV 276

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           I  NRS   G +T  SAT  V+G  F+A+ +TFQNTAGP + QA+A+        +Y+C 
Sbjct: 277 ITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCA 336

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYAR--LFDVQITVTAQSK 184
             GYQDTLYA ++ QFF+EC I G+VDFIFG+   VFQ C I A+  L + + ++TAQ +
Sbjct: 337 MRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGR 396

Query: 185 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 244
              N  +GF  Q  N+    ++  + +    +LGRPW++YS  VF+ +++ + + P GWL
Sbjct: 397 KDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWL 456

Query: 245 EWPG-VPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQ 304
           EW G    + LYY EY NSG GA+  +RV WPGYH LN + E   FTV   I G  WLP 
Sbjct: 457 EWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPS 516

Query: 305 TGIPFRAG 310
           TGI F AG
Sbjct: 517 TGITFIAG 524

BLAST of CsGy4G011410 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.5e-74
Identity = 143/308 (46.43%), Postives = 192/308 (62.34%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           ++ DGT NF  IN+AI+ APN S  R  I+VK G Y E I++P  KT I LIGD S  T 
Sbjct: 240 VAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVTF 299

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           I  NRS G G +T  SATL V+G  F+A+ +   NTAGP + QA+AL   A   A Y+CV
Sbjct: 300 ITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRCV 359

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARL-FDVQITV-TAQSK 184
             GYQDTLY  +  QF++ECDIYG++D+IFGN  VVFQ CNI ++L    Q TV TAQS+
Sbjct: 360 IDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQSR 419

Query: 185 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 244
            + +  +G   QNC++  S ++  S + V  +LGRPWR +S  V ++S++D  +   GW 
Sbjct: 420 DTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGWS 479

Query: 245 EWPGVPE-NLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQ 304
           +W G    + LYY EYNN+G G+ T +RVNWPG+H +   ++   FT   FI G  WL  
Sbjct: 480 KWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFITGDGWLGS 539

Query: 305 TGIPFRAG 310
           T  P+  G
Sbjct: 540 TSFPYDNG 546

BLAST of CsGy4G011410 vs. NCBI nr
Match: XP_011654361.2 (probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 624 bits (1608), Expect = 6.74e-225
Identity = 305/306 (99.67%), Postives = 306/306 (100.00%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           +SLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI
Sbjct: 32  VSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 91

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV
Sbjct: 92  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 151

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 184
           FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS
Sbjct: 152 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 211

Query: 185 LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 244
           LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW
Sbjct: 212 LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 271

Query: 245 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGI 304
           PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGI
Sbjct: 272 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGI 331

Query: 305 PFRAGF 310
           PFRAGF
Sbjct: 332 PFRAGF 337

BLAST of CsGy4G011410 vs. NCBI nr
Match: XP_031741746.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus] >KAE8648510.1 hypothetical protein Csa_009140 [Cucumis sativus])

HSP 1 Score: 607 bits (1566), Expect = 6.23e-219
Identity = 296/310 (95.48%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 1   MEKKLSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDS 60
           MEKKLSLDGT NF+KINDAIAAAPNFSTTRFYIHVKPGTY+EIIEVPYEKTCIALIGDD+
Sbjct: 1   MEKKLSLDGTGNFVKINDAIAAAPNFSTTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDA 60

Query: 61  STTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAY 120
           STTIIVNNRSNGTGSSTASSATLTVNG+NFMAQFLTFQNTAGPNEGQAIA+LDQA HTAY
Sbjct: 61  STTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIAVLDQAKHTAY 120

Query: 121 YKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQ 180
           YKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQ
Sbjct: 121 YKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQ 180

Query: 181 SKPSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKG 240
           SKPSL++LSGFIFQNC VTVSPEIA SKD VTVFLGRPWRMYSMVVFIDSFLDNVVQPKG
Sbjct: 181 SKPSLSALSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKG 240

Query: 241 WLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLP 300
           WLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYH LNNAKEVATFTVE FINGTQWLP
Sbjct: 241 WLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHVLNNAKEVATFTVEIFINGTQWLP 300

Query: 301 QTGIPFRAGF 310
           QTGIPFRAGF
Sbjct: 301 QTGIPFRAGF 310

BLAST of CsGy4G011410 vs. NCBI nr
Match: XP_031745525.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 608 bits (1567), Expect = 1.20e-218
Identity = 298/306 (97.39%), Postives = 299/306 (97.71%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           +SLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI
Sbjct: 32  VSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 91

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           IVNNRSNGTGSSTASSATLTVNG       LTFQNTAGPNEGQAIALLDQATHTAYYKCV
Sbjct: 92  IVNNRSNGTGSSTASSATLTVNGIKLHGSVLTFQNTAGPNEGQAIALLDQATHTAYYKCV 151

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 184
           FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS
Sbjct: 152 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 211

Query: 185 LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 244
           LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW
Sbjct: 212 LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 271

Query: 245 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGI 304
           PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGI
Sbjct: 272 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGI 331

Query: 305 PFRAGF 310
           PFRAGF
Sbjct: 332 PFRAGF 337

BLAST of CsGy4G011410 vs. NCBI nr
Match: XP_008465826.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 575 bits (1482), Expect = 6.65e-206
Identity = 280/308 (90.91%), Postives = 295/308 (95.78%), Query Frame = 0

Query: 3   KKLSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSST 62
           +KLSLDGT NF+K+NDAIAAAPNFS TRFYIHVKPGTYQEIIEVP EKT IALIGDDSST
Sbjct: 17  RKLSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSST 76

Query: 63  TIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYK 122
           TIIVNNRSNGTGSSTASSATLTV G NFMAQFLTFQN+AGPNEGQAIA+LDQA HTAYY+
Sbjct: 77  TIIVNNRSNGTGSSTASSATLTVYGENFMAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYR 136

Query: 123 CVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK 182
           CVFLGYQDTLYAG++PQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK
Sbjct: 137 CVFLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK 196

Query: 183 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 242
            SL++ SGFIFQNCNVTVSPEIA SKD VTVFLGRPWRMYSMVVFIDSFLD+VVQPKGWL
Sbjct: 197 QSLSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWL 256

Query: 243 EWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQT 302
           EWPGVPEN+LYYAEYNNSGGGANTSQRVNWPGYH L+NA+EVATF+VE FINGTQWLPQT
Sbjct: 257 EWPGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQT 316

Query: 303 GIPFRAGF 310
           GIPFRAGF
Sbjct: 317 GIPFRAGF 324

BLAST of CsGy4G011410 vs. NCBI nr
Match: KAA0040551.1 (putative pectinesterase/pectinesterase inhibitor 39 [Cucumis melo var. makuwa] >TYK05594.1 putative pectinesterase/pectinesterase inhibitor 39 [Cucumis melo var. makuwa])

HSP 1 Score: 571 bits (1471), Expect = 2.43e-204
Identity = 278/308 (90.26%), Postives = 294/308 (95.45%), Query Frame = 0

Query: 3   KKLSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSST 62
           K +SLDGT NF+K+NDAIAAAPNFS TRFYIHVKPGTYQEIIEVP EKT IALIGDDSST
Sbjct: 10  KLVSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSST 69

Query: 63  TIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYK 122
           TIIVNNRSNGTGSSTASSATLTV G NFMA+FLTFQN+AGPNEGQAIA+LDQA HTAYY+
Sbjct: 70  TIIVNNRSNGTGSSTASSATLTVYGENFMARFLTFQNSAGPNEGQAIAVLDQAKHTAYYR 129

Query: 123 CVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK 182
           CVFLGYQDTLYAG++PQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK
Sbjct: 130 CVFLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK 189

Query: 183 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 242
            SL++ SGFIFQNCNVTVSPEIA SKD VTVFLGRPWRMYSMVVFIDSFLD+VVQPKGWL
Sbjct: 190 QSLSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWL 249

Query: 243 EWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQT 302
           EWPGVPEN+LYYAEYNNSGGGANTSQRVNWPGYH L+NA+EVATF+VE FINGTQWLPQT
Sbjct: 250 EWPGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQT 309

Query: 303 GIPFRAGF 310
           GIPFRAGF
Sbjct: 310 GIPFRAGF 317

BLAST of CsGy4G011410 vs. ExPASy TrEMBL
Match: A0A0A0L1M3 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 613 bits (1582), Expect = 2.31e-221
Identity = 301/306 (98.37%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           +SLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI
Sbjct: 25  VSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 84

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV
Sbjct: 85  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 144

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 184
           FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS
Sbjct: 145 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 204

Query: 185 LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 244
           LNSLSGFIFQNC VTVSPEIA SKD VTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW
Sbjct: 205 LNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 264

Query: 245 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGI 304
           PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVE FINGTQWLPQTGI
Sbjct: 265 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVEIFINGTQWLPQTGI 324

Query: 305 PFRAGF 310
           PFRAGF
Sbjct: 325 PFRAGF 330

BLAST of CsGy4G011410 vs. ExPASy TrEMBL
Match: A0A1S3CQ52 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503419 PE=4 SV=1)

HSP 1 Score: 575 bits (1482), Expect = 3.22e-206
Identity = 280/308 (90.91%), Postives = 295/308 (95.78%), Query Frame = 0

Query: 3   KKLSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSST 62
           +KLSLDGT NF+K+NDAIAAAPNFS TRFYIHVKPGTYQEIIEVP EKT IALIGDDSST
Sbjct: 17  RKLSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSST 76

Query: 63  TIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYK 122
           TIIVNNRSNGTGSSTASSATLTV G NFMAQFLTFQN+AGPNEGQAIA+LDQA HTAYY+
Sbjct: 77  TIIVNNRSNGTGSSTASSATLTVYGENFMAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYR 136

Query: 123 CVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK 182
           CVFLGYQDTLYAG++PQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK
Sbjct: 137 CVFLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK 196

Query: 183 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 242
            SL++ SGFIFQNCNVTVSPEIA SKD VTVFLGRPWRMYSMVVFIDSFLD+VVQPKGWL
Sbjct: 197 QSLSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWL 256

Query: 243 EWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQT 302
           EWPGVPEN+LYYAEYNNSGGGANTSQRVNWPGYH L+NA+EVATF+VE FINGTQWLPQT
Sbjct: 257 EWPGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQT 316

Query: 303 GIPFRAGF 310
           GIPFRAGF
Sbjct: 317 GIPFRAGF 324

BLAST of CsGy4G011410 vs. ExPASy TrEMBL
Match: A0A5D3C2R6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00440 PE=4 SV=1)

HSP 1 Score: 571 bits (1471), Expect = 1.18e-204
Identity = 278/308 (90.26%), Postives = 294/308 (95.45%), Query Frame = 0

Query: 3   KKLSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSST 62
           K +SLDGT NF+K+NDAIAAAPNFS TRFYIHVKPGTYQEIIEVP EKT IALIGDDSST
Sbjct: 10  KLVSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSST 69

Query: 63  TIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYK 122
           TIIVNNRSNGTGSSTASSATLTV G NFMA+FLTFQN+AGPNEGQAIA+LDQA HTAYY+
Sbjct: 70  TIIVNNRSNGTGSSTASSATLTVYGENFMARFLTFQNSAGPNEGQAIAVLDQAKHTAYYR 129

Query: 123 CVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK 182
           CVFLGYQDTLYAG++PQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK
Sbjct: 130 CVFLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK 189

Query: 183 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 242
            SL++ SGFIFQNCNVTVSPEIA SKD VTVFLGRPWRMYSMVVFIDSFLD+VVQPKGWL
Sbjct: 190 QSLSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWL 249

Query: 243 EWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQT 302
           EWPGVPEN+LYYAEYNNSGGGANTSQRVNWPGYH L+NA+EVATF+VE FINGTQWLPQT
Sbjct: 250 EWPGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQT 309

Query: 303 GIPFRAGF 310
           GIPFRAGF
Sbjct: 310 GIPFRAGF 317

BLAST of CsGy4G011410 vs. ExPASy TrEMBL
Match: A0A6J1KPG6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1)

HSP 1 Score: 497 bits (1280), Expect = 2.92e-175
Identity = 237/305 (77.70%), Postives = 269/305 (88.20%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           +SLDGT NF+KI+DAI AAPNF+TTRFYIH+KPG Y E IE+P +KT I+LIGD++STTI
Sbjct: 31  VSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIYNEHIEIPSQKTFISLIGDNASTTI 90

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           IV+NRS  TG STA+SATLTVNG+NFMA+FLTFQN+AGP  GQA+A+LDQA HTAYYKCV
Sbjct: 91  IVDNRSKATGFSTANSATLTVNGANFMAKFLTFQNSAGPKMGQAVAVLDQAKHTAYYKCV 150

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 184
           FLGYQDTLYA A+PQFFKECDIYG+VDFIFGNGLVVFQDCNIYARL D+Q TVTAQSK +
Sbjct: 151 FLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVVFQDCNIYARLVDIQSTVTAQSKAN 210

Query: 185 LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 244
           L+  SGF+FQNC VTVSPEIA S+D V VFLGRPWR YSMV F++SFLDNVVQPKGWLEW
Sbjct: 211 LDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWRAYSMVAFVESFLDNVVQPKGWLEW 270

Query: 245 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGI 304
            GVP NLLYY EYNN GGGANTSQRV+WPG+H L++  +VA FTVE FINGT WLPQT I
Sbjct: 271 LGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHSLTDVAKFTVENFINGTTWLPQTTI 330

Query: 305 PFRAG 309
           PFR+G
Sbjct: 331 PFRSG 335

BLAST of CsGy4G011410 vs. ExPASy TrEMBL
Match: A0A6J1GIG0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 1.69e-174
Identity = 235/305 (77.05%), Postives = 268/305 (87.87%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           +SLDGT NF+KI+DAI AAPNF+TTRFYIH+KPG Y E IEVP++KT I+LIGD++STTI
Sbjct: 31  VSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIYNEHIEVPFQKTFISLIGDNASTTI 90

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           IV+NRS   G ST++SATLTVNG+NFMA+FLTFQN+AGP  GQA+A+LDQA HTAYYKCV
Sbjct: 91  IVDNRSKAAGFSTSNSATLTVNGANFMAKFLTFQNSAGPKMGQAVAVLDQAKHTAYYKCV 150

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 184
           FLGYQDTLYA A+PQFFKECDIYG+VDFIFGNGLVVFQDCNIYARL D+  TVTAQSK +
Sbjct: 151 FLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVVFQDCNIYARLVDIHTTVTAQSKAN 210

Query: 185 LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 244
           L+  SGF+FQNC VTVSPEIA S+D V VFLGRPWR YSMV F++SFLDNVVQPKGWLEW
Sbjct: 211 LDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWRAYSMVAFVESFLDNVVQPKGWLEW 270

Query: 245 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTGI 304
            GVP NLLYY EYNN GGGANTSQRV+WPG+H L++  +VA FTVE FINGT WLP+T I
Sbjct: 271 LGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHSLTDVANFTVENFINGTTWLPETTI 330

Query: 305 PFRAG 309
           PFRAG
Sbjct: 331 PFRAG 335

BLAST of CsGy4G011410 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 295.8 bits (756), Expect = 4.0e-80
Identity = 145/309 (46.93%), Postives = 198/309 (64.08%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           +++DGT NF  INDA+ AAPN S TRF I++K G Y E +E+P +KT I  IGD    T+
Sbjct: 224 VAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTV 283

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           I  NRS   G ST  + T+ V G  ++A+ ++F N+AGP + QA+A    + H+A+Y+C 
Sbjct: 284 IKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCE 343

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDV--QITVTAQSK 184
           F GYQDTLY  +  QF++ECDIYG++DFIFGN  VVFQ+ ++YAR  +   +I  TAQS+
Sbjct: 344 FDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSR 403

Query: 185 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 244
              +  +G    NC +  +P++ P K+N   +LGRPWR YS  V I SF+D+++ P GWL
Sbjct: 404 NQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWL 463

Query: 245 EW-PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQ 304
           E         LYY EY N G GAN ++RV WPG+  + N  E   FTV  FI+G+ WL  
Sbjct: 464 EGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNS 523

Query: 305 TGIPFRAGF 311
           TGIPF  GF
Sbjct: 524 TGIPFSLGF 532

BLAST of CsGy4G011410 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 287.0 bits (733), Expect = 1.9e-77
Identity = 140/308 (45.45%), Postives = 197/308 (63.96%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           ++LDGT NF KI DAI  AP++S+TRF I++K G Y E +E+  +K  I ++GD    T+
Sbjct: 215 VALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDGIDVTV 274

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           I  NRS   G +T  SAT  V+G  F+A+ +TFQNTAGP + QA+AL   +  + +++C 
Sbjct: 275 ISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSVFFRCA 334

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYAR--LFDVQITVTAQSK 184
             GYQDTLY   + QF++EC I G+VDFIFG+G VVFQ+C I A+  L + + T+TAQ +
Sbjct: 335 MRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTITAQGR 394

Query: 185 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 244
             +N  SGF  Q  N++   ++ P  +    +LGRPW++YS  VFI + + +VV+P+GWL
Sbjct: 395 KDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWL 454

Query: 245 EW-PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQ 304
           EW      + L+Y E+ N G G+  S RV WPGYH  NN+ +   FTV  FI G  WLP 
Sbjct: 455 EWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKGNLWLPS 514

Query: 305 TGIPFRAG 310
           TG+ F  G
Sbjct: 515 TGVTFSDG 522

BLAST of CsGy4G011410 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 283.1 bits (723), Expect = 2.7e-76
Identity = 139/308 (45.13%), Postives = 192/308 (62.34%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           ++ DGT NF  I+DA+ AAP++ST R+ IHVK G Y E +E+  +K  I ++GD    T+
Sbjct: 217 VAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATV 276

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           I  NRS   G +T  SAT  V+G  F+A+ +TFQNTAGP + QA+A+        +Y+C 
Sbjct: 277 ITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCA 336

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYAR--LFDVQITVTAQSK 184
             GYQDTLYA ++ QFF+EC I G+VDFIFG+   VFQ C I A+  L + + ++TAQ +
Sbjct: 337 MRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGR 396

Query: 185 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 244
              N  +GF  Q  N+    ++  + +    +LGRPW++YS  VF+ +++ + + P GWL
Sbjct: 397 KDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWL 456

Query: 245 EWPG-VPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQ 304
           EW G    + LYY EY NSG GA+  +RV WPGYH LN + E   FTV   I G  WLP 
Sbjct: 457 EWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPS 516

Query: 305 TGIPFRAG 310
           TGI F AG
Sbjct: 517 TGITFIAG 524

BLAST of CsGy4G011410 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 279.3 bits (713), Expect = 3.9e-75
Identity = 143/308 (46.43%), Postives = 192/308 (62.34%), Query Frame = 0

Query: 5   LSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTI 64
           ++ DGT NF  IN+AI+ APN S  R  I+VK G Y E I++P  KT I LIGD S  T 
Sbjct: 240 VAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVTF 299

Query: 65  IVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 124
           I  NRS G G +T  SATL V+G  F+A+ +   NTAGP + QA+AL   A   A Y+CV
Sbjct: 300 ITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRCV 359

Query: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARL-FDVQITV-TAQSK 184
             GYQDTLY  +  QF++ECDIYG++D+IFGN  VVFQ CNI ++L    Q TV TAQS+
Sbjct: 360 IDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQSR 419

Query: 185 PSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 244
            + +  +G   QNC++  S ++  S + V  +LGRPWR +S  V ++S++D  +   GW 
Sbjct: 420 DTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGWS 479

Query: 245 EWPGVPE-NLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQ 304
           +W G    + LYY EYNN+G G+ T +RVNWPG+H +   ++   FT   FI G  WL  
Sbjct: 480 KWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFITGDGWLGS 539

Query: 305 TGIPFRAG 310
           T  P+  G
Sbjct: 540 TSFPYDNG 546

BLAST of CsGy4G011410 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 278.5 bits (711), Expect = 6.6e-75
Identity = 142/306 (46.41%), Postives = 193/306 (63.07%), Query Frame = 0

Query: 10  TENFIKINDAIAAAPNFSTTR---FYIHVKPGTYQEIIEVPYEKTCIALIGDDSSTTIIV 69
           ++NF  I DAIAAAPN +      F I+ + G Y+E I VP  K  + L+GD  + TII 
Sbjct: 310 SDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIIT 369

Query: 70  NNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFL 129
            N +   G +T + ++  V G  FMA  +TF+NTAGP + QA+AL + A  +++Y+C F 
Sbjct: 370 GNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFE 429

Query: 130 GYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYAR--LFDVQITVTAQSKPS 189
           GYQDTLY  +L QF++ECDIYG+VDFIFGN   +FQ+CNIYAR  +   +  +TA  +  
Sbjct: 430 GYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLD 489

Query: 190 LNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 249
            N  +G    NC +  +P++A    +   FLGRPW+ YS  VF+ S++ ++VQP GWLEW
Sbjct: 490 PNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEW 549

Query: 250 PG-VPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVETFINGTQWLPQTG 309
            G +  + +YY EY+N G GANT+QRV W GY+ LN A E   FTV  F  G  WLPQT 
Sbjct: 550 NGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLA-EAMNFTVYNFTMGDTWLPQTD 609

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O814155.6e-7946.93Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
O048874.8e-7847.37Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
Q9SMY72.6e-7645.45Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3... [more]
Q9LXK73.8e-7545.13Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3... [more]
O487115.5e-7446.43Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
XP_011654361.26.74e-22599.67probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
XP_031741746.16.23e-21995.48probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus] >KAE864851... [more]
XP_031745525.11.20e-21897.39probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
XP_008465826.16.65e-20690.91PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo][more]
KAA0040551.12.43e-20490.26putative pectinesterase/pectinesterase inhibitor 39 [Cucumis melo var. makuwa] >... [more]
Match NameE-valueIdentityDescription
A0A0A0L1M32.31e-22198.37Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1[more]
A0A1S3CQ523.22e-20690.91Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503419 PE=4 SV=1[more]
A0A5D3C2R61.18e-20490.26Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00440 ... [more]
A0A6J1KPG62.92e-17577.70Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1[more]
A0A6J1GIG01.69e-17477.05Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02300.14.0e-8046.93Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33220.11.9e-7745.45pectin methylesterase 44 [more]
AT3G43270.12.7e-7645.13Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26440.13.9e-7546.43Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.26.6e-7546.41Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 6..295
e-value: 6.1E-102
score: 340.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 4..310
e-value: 3.5E-108
score: 363.8
NoneNo IPR availablePANTHERPTHR31707:SF149PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATEDcoord: 5..308
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 5..308
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 146..155
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 27..46
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 7..308

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G011410.2CsGy4G011410.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity