CsGy4G004070 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G004070
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionUbiquitin carboxyl-terminal hydrolase 12-like isoform X2
LocationGy14Chr4: 2636225 .. 2638464 (+)
RNA-Seq ExpressionCsGy4G004070
SyntenyCsGy4G004070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCAAATCAAACATTGAGATTATTATGGATGATAATATGTCATATGATGATACTACAAAAATAGATGAACGACAACACGGTATATTACTACTTCAAATTATTTTATTATATTGTTAACTTTATATCCACTTCTCTTAGTGTCCAATATTTTTTTCGATTATGTTATTTGCAACCTAAGTACACAAGGTTTGGCAAACTTTAGAAGAAAAGAATAATAACCCAAATTATTTTGGTTTACTAATGTTGATATTATTTAATTTAATTTGCATATCTTTTTTTTTATGCAGAAATTACAATTAAATCGAGGAGGGTTGATCCACCATCTCATTATGCATTTCAAATTGATTCTTACTCGGTGCTTTCACAAATTGAGATGAAGAAATGTGAATCTGGAGACTTTGAAGTTGACGGCTACAAGTGGTAAATTTGGGAACCAAACTTATAATTTTGAAAATACTTTATATTTTAGATTGAAATTAAACATGATTTAATATATCATATCAACTAGGGTTTAAGGCATCATAATTAATTAATCTCTCTATTTCTTATATATATGTGTGTGCTAGGAAATTAATTTTATATCCAAATGGAAACGAAGAAGTGGAAGATCATATTTCTCTCTTCTTGGCTGTATCAACTAATGATAATAATCTTCCTCTTGGTTGGGAACTGAGGGTAATTTTCAGATTCTTTATATTTGATCAAATCAGAGATAACTATTTAACAATCCAAGGTAATTTTTTTCATATTACTTTTCTAATTATATGGTGTTCTTACTGATCTTGTTGTCATCTGCATGTTTCTTGTTAAAAATATTAAAGGTCGAGAGACTCAGGCGAACCTATAATAAAATAAACAAAACCCTAAACCCTAGAAATATGTATTGGTTTTGCAGATGGGAAAATGAGGAAGTATTCTAAAATGAAGAGTGAACATGGATTCACTCACTTGATTTCTCATAATGTTTTCAATAAAGCCTCCAGTGGCTTCCTTGTGAGTAATTGTTGTACATTTGGGGTTGAAGTTTCTATACTTAAAGCCTCAAACAAGGGAGAGAGATTAACCATTTTAAAAGAGCCTCAACAAGACACTTATTTTTGGACTCTATACTCATTTTCTGCTTTAAAGCAACCATTTTATATCTCAGAACCCTTCAATGTCAAAGGAAGAAAATGGTTAGTGAAAAAAGCTCCTGTAAACTAATCTTTTGTGTTTTATCTTTTTCAAAAACTACCAATTTAAATGCTTAAAATTTTAGGAGTTATGTATCAAATTAAACCCTAAATTTATTTTGTGGATGCATCAATTTATGCCATTGATTACATAGTTTAGAAAAATCTAGCGTGAAAGTTTTGGTTTTGAACAAGAAACTAAAAGGACGTGATGAATCAATTTATAATTCATCAATGAATGAATGTAACTTTTCTAAGTGATCTGGATTCCCTTGAATTAGTATAGTACAATTTGTAAAAATTGACATTTAAAAAAGTATGCATTTGTTTTGAGAAGTAATGTATTAATAATTTTCAAAGGCACATAGTACTGAATGTATGAAAATTAAAGAGTTTAATTGACTAACTCAAAAACTTAGGATTTAAAATTTATACAATTAATATAACTTTAAAGTTTAAGTATAAATTGAATCTTTACAGAAAAAAAAAAAAAAAAAGTCTGACTCCTTATCTTTGTTTTGATTTATTAGGAGAATGGAGGTGTACCCACATGGTAATTCCTTGGGAAAGACTTCACATATATCTCTTTACTTGAAGTTGGATAGTTCAGAGACCATTCCTTTGGGGAAGAAAATCTATGCAAAATTTATCCTTGGTGTTTATAACTTTTCAGCCAAAAAATATATTGACAAATCATGTAAGGTCTTTTAATTGAAATTAATTTCCAAATATTTTTTTCTCTTTAGGTTCTTATGTTATGTGACATTATTATTATTATTATAATTGGATTTTGCAGATGAACATTGGTATAAAACCCCAGGTCATGGCAATGGTTTTGATGAGTTCTTGTCCAGAAAAGAAATTAGTACTCATTCGCAAAATGATGCTTTCTATCTCAAAGCTAGAATTGTGGCCATGTCAACTGTGGAAGAATTTTGATAATTCATGGGCATCATTTATCTTGAAAGTTTACAATGTCGTCTACGCTTAGACATTATTACTGCACATGATATTGAATTGTGTTTAAAGTAATATAATGTTGGTAAATAAATAATGGACTTGAGCTAG

mRNA sequence

AGCAAATCAAACATTGAGATTATTATGGATGATAATATGTCATATGATGATACTACAAAAATAGATGAACGACAACACGAAATTACAATTAAATCGAGGAGGGTTGATCCACCATCTCATTATGCATTTCAAATTGATTCTTACTCGGTGCTTTCACAAATTGAGATGAAGAAATGTGAATCTGGAGACTTTGAAGTTGACGGCTACAAGTGGAAATTAATTTTATATCCAAATGGAAACGAAGAAGTGGAAGATCATATTTCTCTCTTCTTGGCTGTATCAACTAATGATAATAATCTTCCTCTTGGTTGGGAACTGAGGGTAATTTTCAGATTCTTTATATTTGATCAAATCAGAGATAACTATTTAACAATCCAAGATGGGAAAATGAGGAAGTATTCTAAAATGAAGAGTGAACATGGATTCACTCACTTGATTTCTCATAATGTTTTCAATAAAGCCTCCAGTGGCTTCCTTGTGAGTAATTGTTGTACATTTGGGGTTGAAGTTTCTATACTTAAAGCCTCAAACAAGGGAGAGAGATTAACCATTTTAAAAGAGCCTCAACAAGACACTTATTTTTGGACTCTATACTCATTTTCTGCTTTAAAGCAACCATTTTATATCTCAGAACCCTTCAATGTCAAAGGAAGAAAATGGAGAATGGAGGTGTACCCACATGGTAATTCCTTGGGAAAGACTTCACATATATCTCTTTACTTGAAGTTGGATAGTTCAGAGACCATTCCTTTGGGGAAGAAAATCTATGCAAAATTTATCCTTGGTGTTTATAACTTTTCAGCCAAAAAATATATTGACAAATCATATGAACATTGGTATAAAACCCCAGGTCATGGCAATGGTTTTGATGAGTTCTTGTCCAGAAAAGAAATTAGTACTCATTCGCAAAATGATGCTTTCTATCTCAAAGCTAGAATTGTGGCCATGTCAACTGTGGAAGAATTTTGATAATTCATGGGCATCATTTATCTTGAAAGTTTACAATGTCGTCTACGCTTAGACATTATTACTGCACATGATATTGAATTGTGTTTAAAGTAATATAATGTTGGTAAATAAATAATGGACTTGAGCTAG

Coding sequence (CDS)

ATGGATGATAATATGTCATATGATGATACTACAAAAATAGATGAACGACAACACGAAATTACAATTAAATCGAGGAGGGTTGATCCACCATCTCATTATGCATTTCAAATTGATTCTTACTCGGTGCTTTCACAAATTGAGATGAAGAAATGTGAATCTGGAGACTTTGAAGTTGACGGCTACAAGTGGAAATTAATTTTATATCCAAATGGAAACGAAGAAGTGGAAGATCATATTTCTCTCTTCTTGGCTGTATCAACTAATGATAATAATCTTCCTCTTGGTTGGGAACTGAGGGTAATTTTCAGATTCTTTATATTTGATCAAATCAGAGATAACTATTTAACAATCCAAGATGGGAAAATGAGGAAGTATTCTAAAATGAAGAGTGAACATGGATTCACTCACTTGATTTCTCATAATGTTTTCAATAAAGCCTCCAGTGGCTTCCTTGTGAGTAATTGTTGTACATTTGGGGTTGAAGTTTCTATACTTAAAGCCTCAAACAAGGGAGAGAGATTAACCATTTTAAAAGAGCCTCAACAAGACACTTATTTTTGGACTCTATACTCATTTTCTGCTTTAAAGCAACCATTTTATATCTCAGAACCCTTCAATGTCAAAGGAAGAAAATGGAGAATGGAGGTGTACCCACATGGTAATTCCTTGGGAAAGACTTCACATATATCTCTTTACTTGAAGTTGGATAGTTCAGAGACCATTCCTTTGGGGAAGAAAATCTATGCAAAATTTATCCTTGGTGTTTATAACTTTTCAGCCAAAAAATATATTGACAAATCATATGAACATTGGTATAAAACCCCAGGTCATGGCAATGGTTTTGATGAGTTCTTGTCCAGAAAAGAAATTAGTACTCATTCGCAAAATGATGCTTTCTATCTCAAAGCTAGAATTGTGGCCATGTCAACTGTGGAAGAATTTTGA

Protein sequence

MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDGYKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEPQQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSETIPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAFYLKARIVAMSTVEEF*
Homology
BLAST of CsGy4G004070 vs. ExPASy Swiss-Prot
Match: Q9M2J1 (MATH domain and coiled-coil domain-containing protein At3g58250 OS=Arabidopsis thaliana OX=3702 GN=At3g58250 PE=4 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 3.0e-11
Identity = 38/123 (30.89%), Postives = 66/123 (53.66%), Query Frame = 0

Query: 44  SQIEMKKCESGDFEVDGYKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFR 103
           S ++ +K  S  F +DG +W+L+ +P GN+   DH+SL+L V+ ++ +LP GW     F 
Sbjct: 19  SSLQSEKIYSDQFVIDGCRWRLLAFPKGNDTKSDHLSLYLDVAESE-SLPCGWRRHAQFS 78

Query: 104 FFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVS 163
           F I + I +   + +   +  + +  S+ GFT+L+         SGFLV       VE+ 
Sbjct: 79  FTIVNHIPEK-CSQRKETIHWFCEKVSDWGFTNLVPLIELKAEDSGFLVKGELKIVVEIE 138

Query: 164 ILK 167
           +L+
Sbjct: 139 VLE 139

BLAST of CsGy4G004070 vs. ExPASy Swiss-Prot
Match: Q9M2I9 (MATH domain and coiled-coil domain-containing protein At3g58270 OS=Arabidopsis thaliana OX=3702 GN=At3g58270 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 4.3e-10
Identity = 41/127 (32.28%), Postives = 67/127 (52.76%), Query Frame = 0

Query: 44  SQIEMKKCESGDFEVDGYKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFR 103
           S  + +K  S +F VDG KW+L+ +P GN    + +SL+LAV+ ++  LP GW     F 
Sbjct: 17  SSQQSRKNYSDEFFVDGCKWRLLAFPKGNG--VEKLSLYLAVAGSE-FLPDGWRRHAYFH 76

Query: 104 FFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVS 163
           F + +Q+ D     ++ K   +    S+ GFT ++S    +    GFLV+      V+VS
Sbjct: 77  FSVVNQLSDELSQARETK-NWFDASTSDWGFTSMLSLKKLHDKDGGFLVNGELKIVVDVS 136

Query: 164 ILKASNK 171
           +L+   K
Sbjct: 137 VLEVIGK 139

BLAST of CsGy4G004070 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 1.6e-09
Identity = 40/147 (27.21%), Postives = 72/147 (48.98%), Query Frame = 0

Query: 175 TILKEPQQD----TYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHIS 234
           T+  +P +D     + WT+ +FS      + S+ F V G KWR+ ++P GN++    H+S
Sbjct: 43  TVENQPAEDPPTLKFTWTIPNFSRQNTRKHYSDVFVVGGYKWRILIFPKGNNV---DHLS 102

Query: 235 LYLKLDSSETIPLGKKIYAKFILGVYNFSAKKY-IDKSYEHWYKTPGHGNGFDEFLSRKE 294
           +YL +  + ++P G   YA+F L V N    +Y + K  +H +       GF  F+   E
Sbjct: 103 MYLDVSDAASLPYGWSRYAQFSLAVVNQIHTRYTVRKETQHQFNARESDWGFTSFMPLSE 162

Query: 295 ISTHSQ----NDAFYLKARIVAMSTVE 313
           +   S+    ND   ++A +     ++
Sbjct: 163 LYDPSRGYLVNDTVLVEAEVAVRKVLD 186

BLAST of CsGy4G004070 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 8.1e-09
Identity = 37/140 (26.43%), Postives = 68/140 (48.57%), Query Frame = 0

Query: 178 KEPQQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDS 237
           ++P    + WT+  F+ L    + S+ F V G KWR+ ++P GN++    H+S+YL +  
Sbjct: 49  EDPPSLKFTWTIPMFTRLNTRKHYSDVFVVGGYKWRILIFPKGNNV---DHLSMYLDVAD 108

Query: 238 SETIPLGKKIYAKFILGVYNFSAKKY-IDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQ- 297
           +  +P G   Y++F L V N    +Y I K  +H +       GF  F+   E+   ++ 
Sbjct: 109 AANLPYGWSRYSQFSLAVVNQVNNRYSIRKETQHQFNARESDWGFTSFMPLSELYEPTRG 168

Query: 298 ---NDAFYLKARIVAMSTVE 313
              ND   ++A +     ++
Sbjct: 169 YLVNDTVLIEAEVAVRKVLD 185

BLAST of CsGy4G004070 vs. ExPASy Swiss-Prot
Match: F4J4A1 (MATH domain and coiled-coil domain-containing protein At3g44800 OS=Arabidopsis thaliana OX=3702 GN=At3g44800 PE=4 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 6.9e-08
Identity = 42/139 (30.22%), Postives = 67/139 (48.20%), Query Frame = 0

Query: 44  SQIEMKKCESGDFEVDGYKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFR 103
           S ++ +  +S  F + G KW L+ YPNG +    ++SL+L   T    LP G   R+ FR
Sbjct: 14  SSLQSEYIKSDIFVIGGCKWCLLAYPNGKQNA-SYLSLYLDGPTL-KTLPCGCRRRIRFR 73

Query: 104 FFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVS 163
             + +Q+ +N     +GK R + K     G+  ++     N    GFLV+N      EV 
Sbjct: 74  LTVVNQLSENLSRRGEGK-RWFDKKLPLCGYEEVLLLTKLNAKHGGFLVNNEVKIVAEVD 133

Query: 164 ILKASNKGERLTILKEPQQ 183
           +L+   K   L + KE Q+
Sbjct: 134 VLEVIGK---LDVSKESQE 146

BLAST of CsGy4G004070 vs. NCBI nr
Match: XP_011653084.1 (MATH domain and coiled-coil domain-containing protein At3g58370 [Cucumis sativus] >KGN53168.1 hypothetical protein Csa_015064 [Cucumis sativus])

HSP 1 Score: 648 bits (1672), Expect = 5.88e-235
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60
           MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG
Sbjct: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60

Query: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG 120
           YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG
Sbjct: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG 120

Query: 121 KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP 180
           KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP
Sbjct: 121 KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP 180

Query: 181 QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 240
           QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET
Sbjct: 181 QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 240

Query: 241 IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAFY 300
           IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAFY
Sbjct: 241 IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAFY 300

Query: 301 LKARIVAMSTVEEF 314
           LKARIVAMSTVEEF
Sbjct: 301 LKARIVAMSTVEEF 314

BLAST of CsGy4G004070 vs. NCBI nr
Match: XP_008453868.1 (PREDICTED: uncharacterized protein LOC103494460 isoform X1 [Cucumis melo] >KAA0044674.1 ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 [Cucumis melo var. makuwa] >TYK16908.1 ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 590 bits (1520), Expect = 8.72e-212
Identity = 286/315 (90.79%), Postives = 303/315 (96.19%), Query Frame = 0

Query: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60
           MDDNMSYD++ K DERQHEITIKSRRVDPPSHYAFQIDSYSVLS+IEMKKCESGDFEVDG
Sbjct: 1   MDDNMSYDESIK-DERQHEITIKSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDG 60

Query: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNN-LPLGWELRVIFRFFIFDQIRDNYLTIQD 120
           Y WKLILYPNGN EVEDHISLFLA+STNDNN LPLGWE+RVIFRFFIFDQIRDNYLTIQD
Sbjct: 61  YMWKLILYPNGNGEVEDHISLFLAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQD 120

Query: 121 GKMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKE 180
           GKMRKYSKMKSEHGFTHLISHNVF+KAS+GFLV+N CTFGVEVSILKASNKGERLTI +E
Sbjct: 121 GKMRKYSKMKSEHGFTHLISHNVFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFRE 180

Query: 181 PQQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSE 240
           PQQDTYFWTLYSFSA     YISEPFNVKGRKWRME+YP+GNSLGKTSHISLYLKLD+SE
Sbjct: 181 PQQDTYFWTLYSFSAKTNQSYISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSE 240

Query: 241 TIPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAF 300
           TIPLGKKIYAKF+LGVYNFSAKKYIDKSYEHW+KTPGHG+GFDEFLSR+EISTHSQNDAF
Sbjct: 241 TIPLGKKIYAKFLLGVYNFSAKKYIDKSYEHWFKTPGHGDGFDEFLSRREISTHSQNDAF 300

Query: 301 YLKARIVAMSTVEEF 314
           YLKARIVAMSTV+EF
Sbjct: 301 YLKARIVAMSTVDEF 314

BLAST of CsGy4G004070 vs. NCBI nr
Match: XP_038902857.1 (MATH domain and coiled-coil domain-containing protein At3g58400-like [Benincasa hispida])

HSP 1 Score: 522 bits (1344), Expect = 5.31e-185
Identity = 252/314 (80.25%), Postives = 279/314 (88.85%), Query Frame = 0

Query: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60
           MDDNMSYDDT   DE+QHEI  +SRRVDPPSHYAF+IDSYSV S+IEMKKCESGDFEVDG
Sbjct: 1   MDDNMSYDDTIN-DEQQHEIITRSRRVDPPSHYAFKIDSYSVFSEIEMKKCESGDFEVDG 60

Query: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG 120
           YKWKLILYPNG+EEV+DHISL+LA+S  DN LPLGWE+RVIFRFFIFDQIRDNYLTIQDG
Sbjct: 61  YKWKLILYPNGDEEVQDHISLYLAISAKDN-LPLGWEVRVIFRFFIFDQIRDNYLTIQDG 120

Query: 121 KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP 180
           KMR+YSKMKSEHGFT L+SHN+FNKAS+GFLV N CTFGVE+S+LKASNKGE LTI KEP
Sbjct: 121 KMRRYSKMKSEHGFTQLLSHNIFNKASNGFLVDNSCTFGVEISVLKASNKGEILTIYKEP 180

Query: 181 QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 240
           QQDT+FW++YSFS     FYISEPFNVKGR+WRM +YP+G+S GKTSHISLYL+LDSSET
Sbjct: 181 QQDTFFWSIYSFSVKINEFYISEPFNVKGRQWRMVIYPNGHSRGKTSHISLYLRLDSSET 240

Query: 241 IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAFY 300
           IPLGKKIYAKF+LGVYNF AKKYIDKS E W+     GNGF EFLSRK++S H QND  Y
Sbjct: 241 IPLGKKIYAKFLLGVYNFVAKKYIDKSKEVWFAATSTGNGFHEFLSRKDMSPHLQNDVLY 300

Query: 301 LKARIVAMSTVEEF 314
           LKARIVAMS VEEF
Sbjct: 301 LKARIVAMSAVEEF 312

BLAST of CsGy4G004070 vs. NCBI nr
Match: XP_023529004.1 (speckle-type POZ protein-like A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 456 bits (1173), Expect = 5.76e-159
Identity = 220/315 (69.84%), Postives = 264/315 (83.81%), Query Frame = 0

Query: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60
           M DNMSYDD  K  E+Q EIT ++RRV+PP++Y FQI+SYS LS+I MKKCESG+F VDG
Sbjct: 1   MVDNMSYDDPIKR-EQQPEIT-RTRRVEPPANYTFQINSYSTLSEIGMKKCESGNFAVDG 60

Query: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG 120
           Y WKLILYPNG++EVEDHISL+LA+ + DN LPLGWE++VIFRFF++D+I+DNYLT+QDG
Sbjct: 61  YNWKLILYPNGDDEVEDHISLYLAIISKDN-LPLGWEVKVIFRFFVYDRIKDNYLTVQDG 120

Query: 121 KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP 180
           KMRKYSKMKSEHGFT  ++HN FN+AS+GFLV N CTFGVEVS+LKASNKGE LTI KEP
Sbjct: 121 KMRKYSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP 180

Query: 181 QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 240
           Q  ++FW++ SFS     FY+SEPF VKGRKWR+ +YP+G S GKTSHISLYLKLDSSET
Sbjct: 181 QLGSFFWSISSFSNKTNEFYLSEPFTVKGRKWRLMIYPNGLSSGKTSHISLYLKLDSSET 240

Query: 241 IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHS-QNDAF 300
           +P GKKIYAKF L VYNF AKKY+D+S E+W+    +G+GF EFLSR ++  H  QND F
Sbjct: 241 VPTGKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVF 300

Query: 301 YLKARIVAMSTVEEF 314
           YLKARIVAMS VE+F
Sbjct: 301 YLKARIVAMSVVEDF 312

BLAST of CsGy4G004070 vs. NCBI nr
Match: KAG6600198.1 (Ubiquitin carboxyl-terminal hydrolase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 456 bits (1173), Expect = 1.25e-157
Identity = 219/315 (69.52%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60
           MDDNMSYDD  K  E+Q EIT ++RRV+PP++Y FQI+SYS LS+I MKKCESG+F VDG
Sbjct: 88  MDDNMSYDDPIKH-EQQPEIT-RTRRVEPPANYTFQINSYSTLSEIGMKKCESGNFAVDG 147

Query: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG 120
           + WKLILYPNG++EVEDHISL+LA+++ DN LPLGWE++VIFRFF++D+I+DNYLT+QDG
Sbjct: 148 FNWKLILYPNGDDEVEDHISLYLAITSKDN-LPLGWEVKVIFRFFVYDRIKDNYLTVQDG 207

Query: 121 KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP 180
           KMRKYSKMKSEHGFT  ++HN FN+AS+GFLV N CTFGVEVS+LKASNKGE LTI KEP
Sbjct: 208 KMRKYSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP 267

Query: 181 QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 240
           Q  ++FW++ SFS     FY+SEPF VKGRKWR+ +YP+G S GK SHISLYLKLDSSET
Sbjct: 268 QLGSFFWSISSFSNKTNEFYLSEPFTVKGRKWRLTIYPNGLSSGKMSHISLYLKLDSSET 327

Query: 241 IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHS-QNDAF 300
           +P GKKIYAKF L VYNF AKKY+D+S EHW+    +G GF EFLSR ++  H  QND F
Sbjct: 328 VPTGKKIYAKFFLSVYNFGAKKYVDQSNEHWFDNTTYGYGFHEFLSRNDLQPHHLQNDVF 387

Query: 301 YLKARIVAMSTVEEF 314
           YLKARI AMS VE+F
Sbjct: 388 YLKARIAAMSVVEDF 399

BLAST of CsGy4G004070 vs. ExPASy TrEMBL
Match: A0A0A0KUE1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G023030 PE=4 SV=1)

HSP 1 Score: 648 bits (1672), Expect = 2.85e-235
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60
           MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG
Sbjct: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60

Query: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG 120
           YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG
Sbjct: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG 120

Query: 121 KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP 180
           KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP
Sbjct: 121 KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP 180

Query: 181 QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 240
           QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET
Sbjct: 181 QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 240

Query: 241 IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAFY 300
           IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAFY
Sbjct: 241 IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAFY 300

Query: 301 LKARIVAMSTVEEF 314
           LKARIVAMSTVEEF
Sbjct: 301 LKARIVAMSTVEEF 314

BLAST of CsGy4G004070 vs. ExPASy TrEMBL
Match: A0A5A7TNH7 (Ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00430 PE=4 SV=1)

HSP 1 Score: 590 bits (1520), Expect = 4.22e-212
Identity = 286/315 (90.79%), Postives = 303/315 (96.19%), Query Frame = 0

Query: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60
           MDDNMSYD++ K DERQHEITIKSRRVDPPSHYAFQIDSYSVLS+IEMKKCESGDFEVDG
Sbjct: 1   MDDNMSYDESIK-DERQHEITIKSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDG 60

Query: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNN-LPLGWELRVIFRFFIFDQIRDNYLTIQD 120
           Y WKLILYPNGN EVEDHISLFLA+STNDNN LPLGWE+RVIFRFFIFDQIRDNYLTIQD
Sbjct: 61  YMWKLILYPNGNGEVEDHISLFLAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQD 120

Query: 121 GKMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKE 180
           GKMRKYSKMKSEHGFTHLISHNVF+KAS+GFLV+N CTFGVEVSILKASNKGERLTI +E
Sbjct: 121 GKMRKYSKMKSEHGFTHLISHNVFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFRE 180

Query: 181 PQQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSE 240
           PQQDTYFWTLYSFSA     YISEPFNVKGRKWRME+YP+GNSLGKTSHISLYLKLD+SE
Sbjct: 181 PQQDTYFWTLYSFSAKTNQSYISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSE 240

Query: 241 TIPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAF 300
           TIPLGKKIYAKF+LGVYNFSAKKYIDKSYEHW+KTPGHG+GFDEFLSR+EISTHSQNDAF
Sbjct: 241 TIPLGKKIYAKFLLGVYNFSAKKYIDKSYEHWFKTPGHGDGFDEFLSRREISTHSQNDAF 300

Query: 301 YLKARIVAMSTVEEF 314
           YLKARIVAMSTV+EF
Sbjct: 301 YLKARIVAMSTVDEF 314

BLAST of CsGy4G004070 vs. ExPASy TrEMBL
Match: A0A1S3BYG6 (uncharacterized protein LOC103494460 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494460 PE=4 SV=1)

HSP 1 Score: 590 bits (1520), Expect = 4.22e-212
Identity = 286/315 (90.79%), Postives = 303/315 (96.19%), Query Frame = 0

Query: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60
           MDDNMSYD++ K DERQHEITIKSRRVDPPSHYAFQIDSYSVLS+IEMKKCESGDFEVDG
Sbjct: 1   MDDNMSYDESIK-DERQHEITIKSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDG 60

Query: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNN-LPLGWELRVIFRFFIFDQIRDNYLTIQD 120
           Y WKLILYPNGN EVEDHISLFLA+STNDNN LPLGWE+RVIFRFFIFDQIRDNYLTIQD
Sbjct: 61  YMWKLILYPNGNGEVEDHISLFLAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQD 120

Query: 121 GKMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKE 180
           GKMRKYSKMKSEHGFTHLISHNVF+KAS+GFLV+N CTFGVEVSILKASNKGERLTI +E
Sbjct: 121 GKMRKYSKMKSEHGFTHLISHNVFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFRE 180

Query: 181 PQQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSE 240
           PQQDTYFWTLYSFSA     YISEPFNVKGRKWRME+YP+GNSLGKTSHISLYLKLD+SE
Sbjct: 181 PQQDTYFWTLYSFSAKTNQSYISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSE 240

Query: 241 TIPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAF 300
           TIPLGKKIYAKF+LGVYNFSAKKYIDKSYEHW+KTPGHG+GFDEFLSR+EISTHSQNDAF
Sbjct: 241 TIPLGKKIYAKFLLGVYNFSAKKYIDKSYEHWFKTPGHGDGFDEFLSRREISTHSQNDAF 300

Query: 301 YLKARIVAMSTVEEF 314
           YLKARIVAMSTV+EF
Sbjct: 301 YLKARIVAMSTVDEF 314

BLAST of CsGy4G004070 vs. ExPASy TrEMBL
Match: A0A6J1JFK1 (uncharacterized protein LOC111486429 OS=Cucurbita maxima OX=3661 GN=LOC111486429 PE=4 SV=1)

HSP 1 Score: 449 bits (1156), Expect = 1.08e-156
Identity = 218/315 (69.21%), Postives = 261/315 (82.86%), Query Frame = 0

Query: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60
           M DNMSYDD  K  E+Q EIT ++ RVDPP++Y FQI+SYS LS+I MKKCESG F VDG
Sbjct: 1   MVDNMSYDDPIKH-EQQPEIT-RTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDG 60

Query: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG 120
           Y WKLIL+PNG+++VEDHISL+LA+++ DN LPLGWE++VIFRFF++DQI+DNYLTIQDG
Sbjct: 61  YNWKLILFPNGDDKVEDHISLYLAITSKDN-LPLGWEVKVIFRFFVYDQIKDNYLTIQDG 120

Query: 121 KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP 180
           KMRKYSKMK+EHGFT  ++HN FN+AS+GFLV N CTFGVEVS+LKASNKGE LTI KEP
Sbjct: 121 KMRKYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP 180

Query: 181 QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 240
           Q  ++FW++ SFS     FY SEPF VKGRKWR+ +YP+G   GKTSHISLYLKLDSSET
Sbjct: 181 QLGSFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSET 240

Query: 241 IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHS-QNDAF 300
           IP GKKIYAKF L VYNF AKKY+D+S E+W+    +G+GF EFLSR ++  H  QND F
Sbjct: 241 IPTGKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVF 300

Query: 301 YLKARIVAMSTVEEF 314
           YLKARIVAMS V++F
Sbjct: 301 YLKARIVAMSVVQDF 312

BLAST of CsGy4G004070 vs. ExPASy TrEMBL
Match: A0A6J1FUL4 (speckle-type POZ protein-like A OS=Cucurbita moschata OX=3662 GN=LOC111447016 PE=4 SV=1)

HSP 1 Score: 447 bits (1150), Expect = 8.82e-156
Identity = 215/315 (68.25%), Postives = 261/315 (82.86%), Query Frame = 0

Query: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60
           M DNMSYDD  K  E+Q EIT ++RR +PP++Y FQI+SYS LS+I MKKCESG+F VDG
Sbjct: 1   MVDNMSYDDPIKH-EQQPEIT-RTRRAEPPANYTFQINSYSTLSEIGMKKCESGNFAVDG 60

Query: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG 120
           + WKLILYPNG++EVEDHISL+LA+++ DN LPLGWE++VIFRFF++D+I+DNYLT+QDG
Sbjct: 61  FNWKLILYPNGDDEVEDHISLYLAITSKDN-LPLGWEVKVIFRFFVYDRIKDNYLTVQDG 120

Query: 121 KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP 180
           KMRKYSKMKSEHGFT  ++HN FN+AS+GFLV N CTFGVEVS+LKASNKGE LTI KEP
Sbjct: 121 KMRKYSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP 180

Query: 181 QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 240
           Q  ++FW++ SFS     FY+SEPF VKGRKWR+ +YP+G S GK SHISLYLKLDSSET
Sbjct: 181 QLGSFFWSISSFSNKTNEFYLSEPFTVKGRKWRLMIYPNGLSSGKMSHISLYLKLDSSET 240

Query: 241 IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHS-QNDAF 300
           +P GKKIYAKF L VY+F AK+Y+D+S EHW+    +G GF EFLSR ++  H  QND F
Sbjct: 241 VPTGKKIYAKFFLSVYDFGAKRYVDQSNEHWFDNTTYGYGFHEFLSRNDLRPHHLQNDGF 300

Query: 301 YLKARIVAMSTVEEF 314
           YLKARI AMS VE+F
Sbjct: 301 YLKARIAAMSVVEDF 312

BLAST of CsGy4G004070 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 193.7 bits (491), Expect = 2.2e-49
Identity = 96/269 (35.69%), Postives = 163/269 (60.59%), Query Frame = 0

Query: 22  IKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDGYKWKLILYPNGN--EEVEDHI 81
           IKS    PP+HY  +I+S+S+L++  +++ E+  FE  GYKWKL+LYPNGN  +  +DH+
Sbjct: 11  IKSISDAPPTHYMVKIESFSLLTKHAIERYETESFEAGGYKWKLVLYPNGNKSKNTKDHV 70

Query: 82  SLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLIS 141
           S++L+++ + ++L  GWE+  +FR ++ DQ +DNYL +Q G  R++  +K E GF   I 
Sbjct: 71  SVYLSLA-DSSSLSPGWEVYAVFRLYLLDQNKDNYLILQ-GNERRFHSVKREWGFDKFIP 130

Query: 142 HNVFNKASSGFLVSNCCTFGVEVSILK--ASNKGERLTILKEPQQDTYFWTLYSFSALKQ 201
              F+ AS+G+L+ + C FG +V + K   S +GE L+++K+     + W + +FS L +
Sbjct: 131 TGTFSDASNGYLMEDTCMFGADVFVSKERRSGRGECLSMIKDATSSKHVWKIENFSKLDK 190

Query: 202 PFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSETIPLGKKIYAKFILGVYN 261
             Y S  F    RKW++E YP G   G  +H+S+YL L   ETI  G KI+ +F + +++
Sbjct: 191 ESYDSNAFFAGDRKWKIEFYPTGTKQGTGTHLSIYLTLVDPETISDGTKIFVEFTIRIFD 250

Query: 262 FSAKKYIDKSYEHWYKTPGHGNGFDEFLS 287
               ++I      W+      +G+ +++S
Sbjct: 251 QLQGRHIAGKVTKWFSRSSSEHGWVKYVS 277

BLAST of CsGy4G004070 vs. TAIR 10
Match: AT5G26280.1 (TRAF-like family protein )

HSP 1 Score: 152.5 bits (384), Expect = 5.6e-37
Identity = 89/291 (30.58%), Postives = 148/291 (50.86%), Query Frame = 0

Query: 26  RVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDGYKWKLILYPNGNEE--VEDHISLFL 85
           R + PS     I S+SV+     +  ES  FE  GYKW+L+LY NGN+     DHISL+ 
Sbjct: 55  REERPSSKIVTITSFSVIKG-RGEPYESSVFEAAGYKWRLVLYVNGNKNDGGNDHISLYA 114

Query: 86  AVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHNVF 145
            +    N+LPLGWE+ V  + F+ +     YLT+ DG +++Y+  K E GF  LI  + F
Sbjct: 115 RIE-ETNSLPLGWEVNVDLKLFVHNGKLHKYLTVTDGLVKRYNNAKKEWGFGQLIPRSTF 174

Query: 146 NKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEPQQDTYFWTLYSFSALKQPFYISE 205
             A+ G+L  +  +FG E+ I+K + + E++T +  P  + + W +  FS L+  FY S+
Sbjct: 175 YNANEGYLDQDTGSFGAEIFIVKPAQQQEKVTFISNPPNNVFTWKILRFSTLEDKFYYSD 234

Query: 206 PFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSETIPLGKKIYAKFILGVYNFSAKKY 265
            F V+ R WR+   P G+  G+   + ++L     +   +    +    L + N  +  +
Sbjct: 235 DFLVEDRYWRLGFNPKGDGGGRPHALPIFLFAQGHKANAVATNTWGAVNLRLKNQRSTNH 294

Query: 266 IDKSYEHWYKT-PGHGNGFDEFLSRKEISTHSQ----NDAFYLKARIVAMS 310
                  WY    G+G G +  +   +++  S+    NDA   +A +V +S
Sbjct: 295 RQIYSAAWYPIGSGYGVGVNNIILLADLNDASKGYLVNDAIIFEAEMVKVS 343

BLAST of CsGy4G004070 vs. TAIR 10
Match: AT5G26260.1 (TRAF-like family protein )

HSP 1 Score: 152.5 bits (384), Expect = 5.6e-37
Identity = 92/313 (29.39%), Postives = 154/313 (49.20%), Query Frame = 0

Query: 4   NMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDGYKW 63
           N SY  T+ +      +T +  R + PS     I S+SV+     +  ES  FE  GYKW
Sbjct: 39  NNSYSLTSNLG-----VTTRELRDERPSSKIVTITSFSVIKD-RGEPYESSIFEAAGYKW 98

Query: 64  KLILYPNGNEE--VEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDGK 123
           +L+LY  GN +  + +HISL+  +   +  LP GWE+ V  + F+ ++    YL++ DG 
Sbjct: 99  RLVLYVKGNPKGGINNHISLYARIEETE-TLPRGWEVNVDLKLFVHNRKLKKYLSVTDGT 158

Query: 124 MRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEPQ 183
           +++Y+  K E GFT LIS   F  A+ G+LV +  +FG E+ I+  + K E++T +  P 
Sbjct: 159 VKRYNDAKKEWGFTQLISLPTFYNANEGYLVQDTASFGAEIFIVNPTEKQEKVTFISNPP 218

Query: 184 QDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSETI 243
            + + W +  FS L+  FY S+ F V  R WR+   P G+  G+   + ++L     +  
Sbjct: 219 DNVFTWKILRFSTLEDKFYYSDDFLVGDRYWRLGFNPKGSGGGRPHALPIFLYAQGHKAN 278

Query: 244 PLGKKIYAKFILGVYNFSAKKYIDKSYEHWYK-TPGHGNGFDEFLSRKEISTHSQ----N 303
            +    +    L + N  +  +       WY     +G G +  +   E+   S+    N
Sbjct: 279 AVVTNTWGAVNLRLKNQRSSNHKQLYSAAWYPIRSDYGVGVNNIILMSELKDASKGYMVN 338

Query: 304 DAFYLKARIVAMS 310
           DA   +A +V +S
Sbjct: 339 DAIIFEAEMVKVS 344

BLAST of CsGy4G004070 vs. TAIR 10
Match: AT4G09770.1 (TRAF-like family protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.8e-35
Identity = 82/274 (29.93%), Postives = 146/274 (53.28%), Query Frame = 0

Query: 26  RVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDGYKWKLILYPNG--NEEVEDHISLFL 85
           R + PS Y  ++  +S + +   +  ES DF+V G+KW+LI YP G   E  +DH+S++ 
Sbjct: 5   REEAPSSYLMKLVGFSEV-KFSHQPYESADFDVGGHKWRLIFYPAGKLEEGGKDHVSIYA 64

Query: 86  AVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHNVF 145
            +     ++ +  EL    +FFI++     Y   QDG M+ Y+K K E GF  ++  + F
Sbjct: 65  RIENVGASMQIDAEL----KFFIYNHNNKQYSVFQDGTMKHYNKEKKECGFAQMLLFSKF 124

Query: 146 NKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEPQQDTYFWTLYSFSAL-KQPFYIS 205
           N   +G++  N C  GVE+ ++K   K ER+   + P ++ + W +  FS +  + +Y S
Sbjct: 125 NDPKNGYIDGNACIVGVEIFVIKPIEKVERVVFTQNPPENKFTWKISHFSYIGDKRYYYS 184

Query: 206 EPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSETIPLGKKIYAKFILGVYNFSAKK 265
           + F V  RKWR+++ P G+   K   +S+Y++  +     +    YAK  L + N     
Sbjct: 185 DEFVVGDRKWRLKISPKGDK--KVRALSVYVQAMAYLPNAVASSTYAKLRLRLLNQKNSN 244

Query: 266 YIDKSYEHWY-KTPGHGNGFDEFLSRKEISTHSQ 296
           +I+K   H+Y +  G G+G  E +S ++++  S+
Sbjct: 245 HIEKRVFHFYSRENGDGSGISELISVEDLNDESK 271

BLAST of CsGy4G004070 vs. TAIR 10
Match: AT4G09770.2 (TRAF-like family protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.8e-35
Identity = 82/274 (29.93%), Postives = 146/274 (53.28%), Query Frame = 0

Query: 26  RVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDGYKWKLILYPNG--NEEVEDHISLFL 85
           R + PS Y  ++  +S + +   +  ES DF+V G+KW+LI YP G   E  +DH+S++ 
Sbjct: 5   REEAPSSYLMKLVGFSEV-KFSHQPYESADFDVGGHKWRLIFYPAGKLEEGGKDHVSIYA 64

Query: 86  AVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHNVF 145
            +     ++ +  EL    +FFI++     Y   QDG M+ Y+K K E GF  ++  + F
Sbjct: 65  RIENVGASMQIDAEL----KFFIYNHNNKQYSVFQDGTMKHYNKEKKECGFAQMLLFSKF 124

Query: 146 NKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEPQQDTYFWTLYSFSAL-KQPFYIS 205
           N   +G++  N C  GVE+ ++K   K ER+   + P ++ + W +  FS +  + +Y S
Sbjct: 125 NDPKNGYIDGNACIVGVEIFVIKPIEKVERVVFTQNPPENKFTWKISHFSYIGDKRYYYS 184

Query: 206 EPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSETIPLGKKIYAKFILGVYNFSAKK 265
           + F V  RKWR+++ P G+   K   +S+Y++  +     +    YAK  L + N     
Sbjct: 185 DEFVVGDRKWRLKISPKGDK--KVRALSVYVQAMAYLPNAVASSTYAKLRLRLLNQKNSN 244

Query: 266 YIDKSYEHWY-KTPGHGNGFDEFLSRKEISTHSQ 296
           +I+K   H+Y +  G G+G  E +S ++++  S+
Sbjct: 245 HIEKRVFHFYSRENGDGSGISELISVEDLNDESK 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2J13.0e-1130.89MATH domain and coiled-coil domain-containing protein At3g58250 OS=Arabidopsis t... [more]
Q9M2I94.3e-1032.28MATH domain and coiled-coil domain-containing protein At3g58270 OS=Arabidopsis t... [more]
Q9FPT11.6e-0927.21Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 ... [more]
Q84WU28.1e-0926.43Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 ... [more]
F4J4A16.9e-0830.22MATH domain and coiled-coil domain-containing protein At3g44800 OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
XP_011653084.15.88e-235100.00MATH domain and coiled-coil domain-containing protein At3g58370 [Cucumis sativus... [more]
XP_008453868.18.72e-21290.79PREDICTED: uncharacterized protein LOC103494460 isoform X1 [Cucumis melo] >KAA00... [more]
XP_038902857.15.31e-18580.25MATH domain and coiled-coil domain-containing protein At3g58400-like [Benincasa ... [more]
XP_023529004.15.76e-15969.84speckle-type POZ protein-like A [Cucurbita pepo subsp. pepo][more]
KAG6600198.11.25e-15769.52Ubiquitin carboxyl-terminal hydrolase 12, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
A0A0A0KUE12.85e-235100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G023030 PE=4 SV=1[more]
A0A5A7TNH74.22e-21290.79Ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 OS=Cucumis melo var. ma... [more]
A0A1S3BYG64.22e-21290.79uncharacterized protein LOC103494460 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1JFK11.08e-15669.21uncharacterized protein LOC111486429 OS=Cucurbita maxima OX=3661 GN=LOC111486429... [more]
A0A6J1FUL48.82e-15668.25speckle-type POZ protein-like A OS=Cucurbita moschata OX=3662 GN=LOC111447016 PE... [more]
Match NameE-valueIdentityDescription
AT3G17380.12.2e-4935.69TRAF-like family protein [more]
AT5G26280.15.6e-3730.58TRAF-like family protein [more]
AT5G26260.15.6e-3729.39TRAF-like family protein [more]
AT4G09770.11.8e-3529.93TRAF-like family protein [more]
AT4G09770.21.8e-3529.93TRAF-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002083MATH/TRAF domainSMARTSM00061math_3coord: 35..143
e-value: 1.6E-5
score: 34.3
coord: 187..290
e-value: 2.6E-6
score: 37.0
IPR002083MATH/TRAF domainPFAMPF00917MATHcoord: 44..164
e-value: 2.5E-7
score: 30.8
coord: 191..305
e-value: 7.9E-12
score: 45.3
IPR002083MATH/TRAF domainPROSITEPS50144MATHcoord: 182..305
score: 23.382574
IPR002083MATH/TRAF domainPROSITEPS50144MATHcoord: 30..162
score: 25.530411
IPR002083MATH/TRAF domainCDDcd00121MATHcoord: 184..305
e-value: 5.75966E-19
score: 79.3454
IPR002083MATH/TRAF domainCDDcd00121MATHcoord: 32..162
e-value: 3.11994E-20
score: 82.8122
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 175..311
e-value: 6.6E-25
score: 89.7
coord: 14..171
e-value: 2.4E-34
score: 120.4
NoneNo IPR availablePANTHERPTHR46162:SF40UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 12-LIKEcoord: 24..311
NoneNo IPR availablePANTHERPTHR46162TRAF-LIKE FAMILY PROTEINcoord: 24..311
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 181..310
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 30..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G004070.2CsGy4G004070.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity
molecular_function GO:0005515 protein binding