CsGy3G036840 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G036840
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionAuxin efflux carrier component
LocationGy14Chr3: 34871598 .. 34874404 (+)
RNA-Seq ExpressionCsGy3G036840
SyntenyCsGy3G036840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGCTTTATTAACAAGGAATTTCTATAGAGAATACGAAAAATTTTCAATCCTTCTTCTGCTGGTCACCATGATTTCTTTGTCTGATGTTTATCATGTTGTGGCAGCCACAGTCCCTCTGTATGTGGCCATGATGTTAGCTTACATATCTGTAAAATGGTGGAAGTTCTTCACAGCTGAGCAATGTTCTGGAATAAACAAGTTTGTGGCAAAATTTTCAATCCCGCTGTTATCTTTTCAAGTCATTTCCGAGAACAATCTCTACAAAATGAACCGCCAGTTGATATTGGCTGACTTTCTTCAGAAAATATTAGCTATTATTCTGTTGGGAGCAGTCACTAAAATCACCTCTCGTGGAGGCTTGAATTGGATTATCACTGGTCTCTCTCTGTCAACCATGCCAAATACTCTAATTCTAGGATTGCCAGTATTGAAGGCCATGTATGGAAGTGAAGCAGATGTGCTTCTTGCCCAGATAGTTGTCTTGCAGAGCATTCTTTGGTATAACATATTACTGTTTCTCTTTGAGTTTACTACCACCAAAGCAGCTTCCTTAGCTCCAGCATCAGAAGCAACAGGTAGGCATTGAAATTTGCTTTTTAAAAATAGTAACAAAGAGAATGAAATCACCTCAACTCAACTTTATAAGCCATTTATGTGTTTGTTTAGGACTTCCCGAAACAAAAATGGTCATATTCATACCAATGTCATGAACTTCGGTAACCATCCCAGTTTAAGTTTTCATGTATTACATTAGGACTTCCCTCAACTGAAATGGGTGTTTTCATGCTATCAACTTCAATAACATTCCTTCATCCTTGTGAAACCAGAATGGAAACTTGCAAAATTTTCTATTGATTGCCTTTTTCCCTGTTTCCTAGATTTTTATTCTCTTTCTACATCCATAGGTCGTGAGTGCTATACTTAGGTCACTGTATTTGGGAGAAATAATACTCAACATAGTTGTTCGAATGCCCTGAAGGTCTTCGGTTCAACCTTCAAGTCACGTCTTGAGGATATGTATTTATTTATGAGTTATTTACGATAATCTTAAGGTCTAGAGAAGTAGAATATATAAACTTTCTAACCTAGTGATACCATTTTGTGTTCTGAAAACATTTTTTCTAATAATATTATTCTTCCTCTGCTCCGTGGACGTATCTAACATAATGTTAGTGAACCACGTATATCTTTACGTCGATCTCTATACGTTTATTGTATTACTTAATTATTGATTTCGCAACGATAGTCTCACATGAATTCATATAGCAAAAATGTGTAAACTAACTTTATTCGGTTCATTTATCCTAGTTATCGTGTTTGTCCTTTTGTAAAGCAGAAATTGAAATAACTCATGAAGGACGACCGAAAGAAGAGATTGAGGAGGCAAGAGATAGAAACGGAAGGAGTTTGAGAACCAAGTCCATTCTCTTAACAGTTGCAAGGAAGCTCATCATCAACCCCAATACTCATGCAACTATATTGGGACTCATTTGGGCAAGCATAAGATTCAGGTAAGTTAGAGATGATAGCTTTCACTTTAAAAAAGCAATAGATGTATTGGGATCATGGAACTAACATTCTGTCAGGTGGGGAGTAAAACTGCCAGAAGTTATTGACAGATCAATATCAATTCTATCAACTGGAGGCCTTGGTATGGCTATGTTCAGCTTAGGTCTTTTCATGGGTTCAAGGACTAGCATTATAGCATGTGGCACAAAGATGACACTGGTGGCCATGGGAATGAAATTCTTAGTGGGGCCTGCACTTATGGCAGCATGCTCACTTGCTCTAGGACTTAGGGGCAAATTGCTCAGAGTGGCAATTGTGCAGGTTTGTTGTAATTTGTATTAATAACAAGTCCACATCCAGGAATTGGCAATTCTGTTTAACGTGCTTGTTTCTTGATCTTTCTTCTTCTTCTTCTTCTTTGGCACAGGCAGCACTTCCTCAAGGAATTGTTCCATTTGTCTTTTCCAAAGAGTATAATATACATCCAGACGTTTTAAGCACAGGGTAACTATTGTAATTTTCATTTCTTATGTCAATTTTAAACAAGATATTGATCCTCTCTTATATTTAAATGTGCTTAGATCACCAACTTTATGCTCTTTATATACTTTATATAAACTATATATAAAAGATTGAAGTGATACTAATGTTAATACTTAGCTCAGTGGTAAAACTGTCATCTAGTCCCCTCTAGACCCCATTCCTTCATTTTCTTCAATATTATTTCTAGTGAACTACAAATCCTCCATCAACAAAATTTCTAGATCTACTGTCGGCTTAGTTGATAGAATTTTGCTTTCGAGCAGTTTAAGTAGAAGCATTATAGTTACATCCCTAAAGTACATACTGATGTGGGGTTTTCTTTTGGTTACAGGGTACTTTTGGGGTTGCTCATTGCACTGCCAGTAGCACTGGCCTATTACTACTTGCTATCATTGTAAAGCATCAATGCAGCCAATGCCACCAAAGGATAACAAGAAATTTAGGACATTGACGCTAAACATCAGTAAAAGACCCATGAAAATATGAAGCTTTTGAGTTAAAAAATTTTTTTGCCATTTTTTTTCCCGAAATGGTATACTGCTGATGATTTTAAGAAGCATGTAAATGTTTGTGCTGAAGATATCAATGAAGGATGATGATATGCAATAAACAAAATAGAAGGAAAAATTACAATGAGTTAAATAGGAAGCAGAGTGAACAGACCTTTGCTACTAATCAGCATCCTGTTGTTTGTATAGTCTTACTATCCATTGTTTGAGTCTGTAGTTTCAAATATCAGCCAGAGACTTGGGAA

mRNA sequence

GTGCTTTATTAACAAGGAATTTCTATAGAGAATACGAAAAATTTTCAATCCTTCTTCTGCTGGTCACCATGATTTCTTTGTCTGATGTTTATCATGTTGTGGCAGCCACAGTCCCTCTGTATGTGGCCATGATGTTAGCTTACATATCTGTAAAATGGTGGAAGTTCTTCACAGCTGAGCAATGTTCTGGAATAAACAAGTTTGTGGCAAAATTTTCAATCCCGCTGTTATCTTTTCAAGTCATTTCCGAGAACAATCTCTACAAAATGAACCGCCAGTTGATATTGGCTGACTTTCTTCAGAAAATATTAGCTATTATTCTGTTGGGAGCAGTCACTAAAATCACCTCTCGTGGAGGCTTGAATTGGATTATCACTGGTCTCTCTCTGTCAACCATGCCAAATACTCTAATTCTAGGATTGCCAGTATTGAAGGCCATGTATGGAAGTGAAGCAGATGTGCTTCTTGCCCAGATAGTTGTCTTGCAGAGCATTCTTTGGTATAACATATTACTGTTTCTCTTTGAGTTTACTACCACCAAAGCAGCTTCCTTAGCTCCAGCATCAGAAGCAACAGAAATTGAAATAACTCATGAAGGACGACCGAAAGAAGAGATTGAGGAGGCAAGAGATAGAAACGGAAGGAGTTTGAGAACCAAGTCCATTCTCTTAACAGTTGCAAGGAAGCTCATCATCAACCCCAATACTCATGCAACTATATTGGGACTCATTTGGGCAAGCATAAGATTCAGGTGGGGAGTAAAACTGCCAGAAGTTATTGACAGATCAATATCAATTCTATCAACTGGAGGCCTTGGTATGGCTATGTTCAGCTTAGGTCTTTTCATGGGTTCAAGGACTAGCATTATAGCATGTGGCACAAAGATGACACTGGTGGCCATGGGAATGAAATTCTTAGTGGGGCCTGCACTTATGGCAGCATGCTCACTTGCTCTAGGACTTAGGGGCAAATTGCTCAGAGTGGCAATTGTGCAGGCAGCACTTCCTCAAGGAATTGTTCCATTTGTCTTTTCCAAAGAGTATAATATACATCCAGACGTTTTAAGCACAGGGGTACTTTTGGGGTTGCTCATTGCACTGCCAGTAGCACTGGCCTATTACTACTTGCTATCATTGTAAAGCATCAATGCAGCCAATGCCACCAAAGGATAACAAGAAATTTAGGACATTGACGCTAAACATCAGTAAAAGACCCATGAAAATATGAAGCTTTTGAGTTAAAAAATTTTTTTGCCATTTTTTTTCCCGAAATGGTATACTGCTGATGATTTTAAGAAGCATGTAAATGTTTGTGCTGAAGATATCAATGAAGGATGATGATATGCAATAAACAAAATAGAAGGAAAAATTACAATGAGTTAAATAGGAAGCAGAGTGAACAGACCTTTGCTACTAATCAGCATCCTGTTGTTTGTATAGTCTTACTATCCATTGTTTGAGTCTGTAGTTTCAAATATCAGCCAGAGACTTGGGAA

Coding sequence (CDS)

ATGATTTCTTTGTCTGATGTTTATCATGTTGTGGCAGCCACAGTCCCTCTGTATGTGGCCATGATGTTAGCTTACATATCTGTAAAATGGTGGAAGTTCTTCACAGCTGAGCAATGTTCTGGAATAAACAAGTTTGTGGCAAAATTTTCAATCCCGCTGTTATCTTTTCAAGTCATTTCCGAGAACAATCTCTACAAAATGAACCGCCAGTTGATATTGGCTGACTTTCTTCAGAAAATATTAGCTATTATTCTGTTGGGAGCAGTCACTAAAATCACCTCTCGTGGAGGCTTGAATTGGATTATCACTGGTCTCTCTCTGTCAACCATGCCAAATACTCTAATTCTAGGATTGCCAGTATTGAAGGCCATGTATGGAAGTGAAGCAGATGTGCTTCTTGCCCAGATAGTTGTCTTGCAGAGCATTCTTTGGTATAACATATTACTGTTTCTCTTTGAGTTTACTACCACCAAAGCAGCTTCCTTAGCTCCAGCATCAGAAGCAACAGAAATTGAAATAACTCATGAAGGACGACCGAAAGAAGAGATTGAGGAGGCAAGAGATAGAAACGGAAGGAGTTTGAGAACCAAGTCCATTCTCTTAACAGTTGCAAGGAAGCTCATCATCAACCCCAATACTCATGCAACTATATTGGGACTCATTTGGGCAAGCATAAGATTCAGGTGGGGAGTAAAACTGCCAGAAGTTATTGACAGATCAATATCAATTCTATCAACTGGAGGCCTTGGTATGGCTATGTTCAGCTTAGGTCTTTTCATGGGTTCAAGGACTAGCATTATAGCATGTGGCACAAAGATGACACTGGTGGCCATGGGAATGAAATTCTTAGTGGGGCCTGCACTTATGGCAGCATGCTCACTTGCTCTAGGACTTAGGGGCAAATTGCTCAGAGTGGCAATTGTGCAGGCAGCACTTCCTCAAGGAATTGTTCCATTTGTCTTTTCCAAAGAGTATAATATACATCCAGACGTTTTAAGCACAGGGGTACTTTTGGGGTTGCTCATTGCACTGCCAGTAGCACTGGCCTATTACTACTTGCTATCATTGTAA

Protein sequence

MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVISENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPKEEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRGKLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL*
Homology
BLAST of CsGy3G036840 vs. ExPASy Swiss-Prot
Match: Q9LFP6 (Auxin efflux carrier component 8 OS=Arabidopsis thaliana OX=3702 GN=PIN8 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.3e-108
Identity = 214/367 (58.31%), Postives = 277/367 (75.48%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS  D+YHVV+ATVPLYV+M L ++S +  K F+ EQC+GINKFVAKFSIPLLSFQ+IS
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKI----TSRGG-LNWIITGLSLSTMPNTLI 120
           ENN +KM+ +LIL+D LQK L +++L  V +       RGG L W+ITGLS+S +PNTLI
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 LGLPVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPAS------EAT 180
           LG+P+L A+YG EA  +L QIVVLQS++WY ILLFLFE    +A   + AS      +  
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFELNAARALPSSGASLEHTGNDQE 180

Query: 181 EIEITHEGRPKEEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRW 240
           E  I  E + +E+ EE      RS+ T  ILL   RKLIINPNT+AT++G+IWA++ FR 
Sbjct: 181 EANIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIGIIWATLHFRL 240

Query: 241 GVKLPEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALM 300
           G  LPE+ID+SI +LS GGLGMAMFSLGLFM S++SIIACGTKM ++ M +KF++GPALM
Sbjct: 241 GWNLPEMIDKSIHLLSDGGLGMAMFSLGLFMASQSSIIACGTKMAIITMLLKFVLGPALM 300

Query: 301 AACSLALGLRGKLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALA 357
            A +  + L+  L +VAI+QAALPQG+VPFVF+KEYN+HP+++STGV+ G+LIALP  LA
Sbjct: 301 IASAYCIRLKSTLFKVAILQAALPQGVVPFVFAKEYNLHPEIISTGVIFGMLIALPTTLA 360

BLAST of CsGy3G036840 vs. ExPASy Swiss-Prot
Match: Q0JJV0 (Probable auxin efflux carrier component 8 OS=Oryza sativa subsp. japonica OX=39947 GN=PIN8 PE=2 SV=2)

HSP 1 Score: 341.7 bits (875), Expect = 1.0e-92
Identity = 184/354 (51.98%), Postives = 238/354 (67.23%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           M+S  D+Y V+ ATVPLYVAM+LAY+S+KWWK FT EQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MVSWKDIYLVLEATVPLYVAMILAYLSIKWWKLFTPEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
             + Y MN +LI +D LQK LA++   A++K       +W+ITG SLST+PNTLI+G+P+
Sbjct: 61  TTDPYDMNIKLIYSDILQKSLALLGFAAISKACCAEKFDWLITGFSLSTLPNTLIVGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180
           LK MYG +A  LL+QIVVLQS++WY +LLFLFE       +   +SE T           
Sbjct: 121 LKGMYGEQAGKLLSQIVVLQSLIWYTLLLFLFELRAANGMATTTSSETT----------- 180

Query: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
                                                 GLIWA + FRW ++LP ++  S
Sbjct: 181 --------------------------------------GLIWALVGFRWHIRLPLIVSNS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300
           I +LS GGLGMAMFSLGLF   +T IIACG K  L+A+ ++F +GPALM   S A+G+RG
Sbjct: 241 IRMLSDGGLGMAMFSLGLFTALQTKIIACGAKRMLLALAIRFFLGPALMGMSSYAIGMRG 300

Query: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLL 355
            LL++AIVQAALPQGIVPFVF+KEYN+  D+LST +++G+++A+PVALAYY+ +
Sbjct: 301 VLLKIAIVQAALPQGIVPFVFAKEYNVQADILSTAIIVGMMVAVPVALAYYFAM 305

BLAST of CsGy3G036840 vs. ExPASy Swiss-Prot
Match: P0C0X5 (Probable auxin efflux carrier component 1b OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1B PE=2 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.4e-81
Identity = 205/554 (37.00%), Postives = 276/554 (49.82%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI++ D+YHV+ A VPLYVAM LAY SV+WW+ F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITVVDLYHVLTAVVPLYVAMTLAYASVRWWRIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN + MN + + AD LQK++ + LL    ++++RG L+W+IT  SLST+PNTL++G+P+
Sbjct: 61  TNNPFAMNLRFLAADTLQKLIVLALLALWCRLSARGSLDWLITLFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADV----LLAQIVVLQSILWYNILLFLFEF---------------------- 180
           LK MY + ADV    L+ QIVVLQ I+WY ++LFLFE+                      
Sbjct: 121 LKGMYAAAADVDSGSLMVQIVVLQCIIWYTLMLFLFEYRGARLLVMEQFPDTAASIVSFR 180

Query: 181 -----------------------------------TTTKAASLAPASEATE--------- 240
                                               +T + S A  S  T+         
Sbjct: 181 VDSDVVSLAGGGGGAAELQAEAEVGDDGRMRVTVRKSTSSRSEAACSHGTQSHSQSMQPR 240

Query: 241 ------IEI--------------------------------------------------- 300
                 +EI                                                   
Sbjct: 241 VSNLSGVEIYSLQSSRNPTPRGSSFNHAEFFNIVGNGKQGDEEKGAAGGGGHSPQPVVGK 300

Query: 301 ------------------------------------------------------------ 357
                                                                       
Sbjct: 301 RKDLHMFVWSSSASPVSERAAAAAAGAVHVFGGGGADHGDAKGAQAYDEYSFGNKNEKDG 360

BLAST of CsGy3G036840 vs. ExPASy Swiss-Prot
Match: Q0IQA5 (Probable auxin efflux carrier component 1d OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1D PE=2 SV=2)

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-80
Identity = 203/558 (36.38%), Postives = 276/558 (49.46%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI++ D+YHV+ A VPLYVAM LAY SV+WW+ F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITVVDLYHVLTAVVPLYVAMTLAYASVRWWRIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN + MN + + AD LQK++ + LL    ++++RG L+W+IT  SLST+PNTL++G+P+
Sbjct: 61  TNNPFAMNLRFLAADTLQKLIVLALLALWCRLSARGSLDWLITLFSLSTLPNTLVMGIPL 120

Query: 121 LKAMY-------GSEADVLLAQIVVLQSILWYNILLFLFEF------------------- 180
           LK MY       G+++  L+ QIVVLQ I+WY ++LFLFE+                   
Sbjct: 121 LKGMYAAAGAAAGADSGSLMVQIVVLQCIIWYTLMLFLFEYRGARLLVMEQFPDTAASIV 180

Query: 181 --------------------------------------TTTKAASLAPASEATE------ 240
                                                  +T + S A  S  T+      
Sbjct: 181 SFRVDSDVVSLAGGGGGAAELQAEAEVGDDGKMRVTVRKSTSSRSEAACSHGTQSHSQSM 240

Query: 241 ---------IEI------------------------------------------------ 300
                    +EI                                                
Sbjct: 241 QPRVSNLSGVEIYSLQSSRNPTPRGSSFNHAEFFNIVGNGKHGDEEKGAAGGGGHSPQPV 300

Query: 301 ------------------------------------------------------------ 357
                                                                       
Sbjct: 301 VGKRKDLHMFVWSSSASPVSERAAAAAAAGAVHVFGGGGADHGDAKGAQAYDEYSFGNKN 360

BLAST of CsGy3G036840 vs. ExPASy Swiss-Prot
Match: Q67UL3 (Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1C PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 6.7e-76
Identity = 206/592 (34.80%), Postives = 270/592 (45.61%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+ +D YHV+ A VPLYVAM+LAY SVKWW+ FT +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITGADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MN + I AD LQK++ + LL   + ++ RG L W IT  SLST+PNTL++G+P+
Sbjct: 61  TNNPYTMNLRFIAADTLQKLIVLALLTLWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEF-------------TTTKAASLAPASE 180
           LK MYG  +  L+ QIVVLQ I+WY ++LF+FE+             T    AS+   ++
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARILITEQFPDTAGAIASIVVDAD 180

Query: 181 A-----------TEIEITHEG--------------------------------------- 240
                       TE E+  +G                                       
Sbjct: 181 VVSLDGRRDMIETEAEVKEDGKIHVTVRRSNASRSDVYSRRSMGFSSTTPRPSNLTNAEI 240

Query: 241 ---------------------------------------------RPKEEIEEA------ 300
                                                        RP    E+A      
Sbjct: 241 YSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFAAGDAFGVRTGATPRPSNYEEDAAAPNKA 300

Query: 301 ---------------------RDRNGR--------------------------------- 357
                                +  NG+                                 
Sbjct: 301 GSKYGQYPAPNPAMAAPPKPKKAANGQAKGEDGKDLHMFVWSSSASPVSDVFGNGAEYND 360

BLAST of CsGy3G036840 vs. NCBI nr
Match: XP_011652244.1 (auxin efflux carrier component 8 isoform X2 [Cucumis sativus] >KGN59594.1 hypothetical protein Csa_001720 [Cucumis sativus])

HSP 1 Score: 656 bits (1692), Expect = 1.38e-236
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180

Query: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS
Sbjct: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300

Query: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356
           KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of CsGy3G036840 vs. NCBI nr
Match: XP_031738517.1 (auxin efflux carrier component 8 isoform X1 [Cucumis sativus])

HSP 1 Score: 651 bits (1680), Expect = 9.65e-235
Identity = 356/357 (99.72%), Postives = 356/357 (99.72%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT-EIEITHEGRP 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT EIEITHEGRP
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATAEIEITHEGRP 180

Query: 181 KEEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KEEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR
Sbjct: 181 KEEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLR 300
           SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLR
Sbjct: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLR 300

Query: 301 GKLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356
           GKLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 GKLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357

BLAST of CsGy3G036840 vs. NCBI nr
Match: XP_008443438.1 (PREDICTED: putative auxin efflux carrier component 5 [Cucumis melo] >TYK25662.1 putative auxin efflux carrier component 5 [Cucumis melo var. makuwa])

HSP 1 Score: 638 bits (1646), Expect = 1.41e-229
Identity = 346/356 (97.19%), Postives = 352/356 (98.88%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMN QLILADFLQKILAIILLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEIT EGR K
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180

Query: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSL+TKSILLTVARKLIINPNTHATILGLIW+SIRFRWGVKLP VIDRS
Sbjct: 181 EEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSLALGLRG
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLRG 300

Query: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356
           +LLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of CsGy3G036840 vs. NCBI nr
Match: XP_038904643.1 (auxin efflux carrier component 8 [Benincasa hispida])

HSP 1 Score: 637 bits (1643), Expect = 4.04e-229
Identity = 343/356 (96.35%), Postives = 353/356 (99.16%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMN QLILADFLQKILAI+LLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIVLLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEI+IT +GR K
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQDGRAK 180

Query: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSL+T+SILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS
Sbjct: 181 EEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300
           I+ILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSLALGLRG
Sbjct: 241 INILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLRG 300

Query: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356
           +LLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of CsGy3G036840 vs. NCBI nr
Match: KAA0053741.1 (putative auxin efflux carrier component 5 [Cucumis melo var. makuwa])

HSP 1 Score: 634 bits (1634), Expect = 9.86e-228
Identity = 346/357 (96.92%), Postives = 352/357 (98.60%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMN QLILADFLQKILAIILLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT-EIEITHEGRP 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT EIEIT EGR 
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATAEIEITQEGRA 180

Query: 181 KEEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KEEIEEARDRNGRSL+TKSILLTVARKLIINPNTHATILGLIW+SIRFRWGVKLP VIDR
Sbjct: 181 KEEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDR 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLR 300
           SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSLALGLR
Sbjct: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLR 300

Query: 301 GKLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356
           G+LLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357

BLAST of CsGy3G036840 vs. ExPASy TrEMBL
Match: A0A0A0LFB7 (Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_3G827360 PE=3 SV=1)

HSP 1 Score: 656 bits (1692), Expect = 6.68e-237
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180

Query: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS
Sbjct: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300

Query: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356
           KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of CsGy3G036840 vs. ExPASy TrEMBL
Match: A0A5D3DQ58 (Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G008260 PE=3 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 6.83e-230
Identity = 346/356 (97.19%), Postives = 352/356 (98.88%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMN QLILADFLQKILAIILLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEIT EGR K
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180

Query: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSL+TKSILLTVARKLIINPNTHATILGLIW+SIRFRWGVKLP VIDRS
Sbjct: 181 EEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSLALGLRG
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLRG 300

Query: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356
           +LLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of CsGy3G036840 vs. ExPASy TrEMBL
Match: A0A1S3B8S2 (Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103487029 PE=3 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 6.83e-230
Identity = 346/356 (97.19%), Postives = 352/356 (98.88%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMN QLILADFLQKILAIILLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEIT EGR K
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180

Query: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSL+TKSILLTVARKLIINPNTHATILGLIW+SIRFRWGVKLP VIDRS
Sbjct: 181 EEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSLALGLRG
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLRG 300

Query: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356
           +LLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of CsGy3G036840 vs. ExPASy TrEMBL
Match: A0A5A7UJS2 (Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001190 PE=3 SV=1)

HSP 1 Score: 634 bits (1634), Expect = 4.77e-228
Identity = 346/357 (96.92%), Postives = 352/357 (98.60%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMN QLILADFLQKILAIILLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT-EIEITHEGRP 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT EIEIT EGR 
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATAEIEITQEGRA 180

Query: 181 KEEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KEEIEEARDRNGRSL+TKSILLTVARKLIINPNTHATILGLIW+SIRFRWGVKLP VIDR
Sbjct: 181 KEEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDR 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLR 300
           SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSLALGLR
Sbjct: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLR 300

Query: 301 GKLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356
           G+LLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357

BLAST of CsGy3G036840 vs. ExPASy TrEMBL
Match: A0A6J1J3G0 (Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111482319 PE=3 SV=1)

HSP 1 Score: 615 bits (1585), Expect = 1.34e-220
Identity = 331/356 (92.98%), Postives = 347/356 (97.47%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMN QLILADFLQKILAI+LLGA+TKITSRGGL+WIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIVLLGAITKITSRGGLHWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180
           LKAMYG+EADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEI+IT E R K
Sbjct: 121 LKAMYGTEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQEERAK 180

Query: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDR+ RSL+T++ILLTVARKLI NPNTHATILGLIWASIRFRWGVKLPEVIDRS
Sbjct: 181 EEIEEARDRSRRSLKTRTILLTVARKLISNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300
           I+ILSTGGLGMAMFSLGLFMGSR+SIIACGTKMTLVAMGMKFL+GPALMAACS  LGLRG
Sbjct: 241 ITILSTGGLGMAMFSLGLFMGSRSSIIACGTKMTLVAMGMKFLMGPALMAACSFPLGLRG 300

Query: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356
           +LL+VAIVQAALPQGIVPFVF+KEYNIHPDVLSTGVLLGLLIALPVAL YYYLLSL
Sbjct: 301 RLLKVAIVQAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLIALPVALVYYYLLSL 356

BLAST of CsGy3G036840 vs. TAIR 10
Match: AT5G15100.1 (Auxin efflux carrier family protein )

HSP 1 Score: 393.7 bits (1010), Expect = 1.6e-109
Identity = 214/367 (58.31%), Postives = 277/367 (75.48%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS  D+YHVV+ATVPLYV+M L ++S +  K F+ EQC+GINKFVAKFSIPLLSFQ+IS
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKI----TSRGG-LNWIITGLSLSTMPNTLI 120
           ENN +KM+ +LIL+D LQK L +++L  V +       RGG L W+ITGLS+S +PNTLI
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 LGLPVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPAS------EAT 180
           LG+P+L A+YG EA  +L QIVVLQS++WY ILLFLFE    +A   + AS      +  
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFELNAARALPSSGASLEHTGNDQE 180

Query: 181 EIEITHEGRPKEEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRW 240
           E  I  E + +E+ EE      RS+ T  ILL   RKLIINPNT+AT++G+IWA++ FR 
Sbjct: 181 EANIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIGIIWATLHFRL 240

Query: 241 GVKLPEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALM 300
           G  LPE+ID+SI +LS GGLGMAMFSLGLFM S++SIIACGTKM ++ M +KF++GPALM
Sbjct: 241 GWNLPEMIDKSIHLLSDGGLGMAMFSLGLFMASQSSIIACGTKMAIITMLLKFVLGPALM 300

Query: 301 AACSLALGLRGKLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALA 357
            A +  + L+  L +VAI+QAALPQG+VPFVF+KEYN+HP+++STGV+ G+LIALP  LA
Sbjct: 301 IASAYCIRLKSTLFKVAILQAALPQGVVPFVFAKEYNLHPEIISTGVIFGMLIALPTTLA 360

BLAST of CsGy3G036840 vs. TAIR 10
Match: AT2G01420.1 (Auxin efflux carrier family protein )

HSP 1 Score: 276.9 bits (707), Expect = 2.2e-74
Identity = 197/612 (32.19%), Postives = 268/612 (43.79%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  D+Y V+ A VPLYVAM+LAY SV+WWK F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            N+ Y MN + + AD LQKI+ ++LL     +T  G L W+IT  SLST+PNTL++G+P+
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEA------------ 180
           L AMYG+ A  L+ Q+VVLQ I+WY +LLFLFE+   K   +    E             
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 -----------TEIEITHEGR--------------------------------------- 240
                      T+ EI ++G+                                       
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLMMTPRPSNLTGAEIYSLSSTPRGS 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 NFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENNAVKYGFYNNTNSSV 300

Query: 301 --------------------------PKEEIEEARDRNGR-------------------- 357
                                     PKE  ++ ++++ +                    
Sbjct: 301 PAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSKASHDAKELHMFVWSSSASPV 360

BLAST of CsGy3G036840 vs. TAIR 10
Match: AT2G01420.2 (Auxin efflux carrier family protein )

HSP 1 Score: 275.4 bits (703), Expect = 6.4e-74
Identity = 197/616 (31.98%), Postives = 268/616 (43.51%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  D+Y V+ A VPLYVAM+LAY SV+WWK F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            N+ Y MN + + AD LQKI+ ++LL     +T  G L W+IT  SLST+PNTL++G+P+
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEA------------ 180
           L AMYG+ A  L+ Q+VVLQ I+WY +LLFLFE+   K   +    E             
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 -----------TEIEITHEGR--------------------------------------- 240
                      T+ EI ++G+                                       
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLMMTPRPSNLTGAEIYSLSSTPRGS 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 NFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENNAVKYGFYNNTNSSV 300

Query: 301 --------------------------PKEEIEEARDRNGR-------------------- 357
                                     PKE  ++ ++++ +                    
Sbjct: 301 PAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSKASHDAKELHMFVWSSSASPV 360

BLAST of CsGy3G036840 vs. TAIR 10
Match: AT1G23080.3 (Auxin efflux carrier family protein )

HSP 1 Score: 272.7 bits (696), Expect = 4.2e-73
Identity = 205/615 (33.33%), Postives = 263/615 (42.76%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  D+Y V+ A +PLYVAM+LAY SV+WWK F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MN + I AD LQK++ + LL      T  G L W IT  SLST+PNTL++G+P+
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEF-------------------------- 180
           L AMYG  +  L+ QIVVLQ I+WY +LLFLFE+                          
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAEIYSLN 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 TTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEESCAMASSPRF 300

Query: 301 -------------------TTTKAASLAP-----------ASEATEIEI----------- 357
                              T  K  S AP           +++A E+ +           
Sbjct: 301 GYYPGGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELHMFVWGSNGSPVS 360

BLAST of CsGy3G036840 vs. TAIR 10
Match: AT1G23080.1 (Auxin efflux carrier family protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.2e-72
Identity = 205/619 (33.12%), Postives = 263/619 (42.49%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  D+Y V+ A +PLYVAM+LAY SV+WWK F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MN + I AD LQK++ + LL      T  G L W IT  SLST+PNTL++G+P+
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEF-------------------------- 180
           L AMYG  +  L+ QIVVLQ I+WY +LLFLFE+                          
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAEIYSLN 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 TTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEESCAMASSPRF 300

Query: 301 -------------------TTTKAASLAP-----------ASEATEIEI----------- 357
                              T  K  S AP           +++A E+ +           
Sbjct: 301 GYYPGGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELHMFVWGSNGSPVS 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFP62.3e-10858.31Auxin efflux carrier component 8 OS=Arabidopsis thaliana OX=3702 GN=PIN8 PE=2 SV... [more]
Q0JJV01.0e-9251.98Probable auxin efflux carrier component 8 OS=Oryza sativa subsp. japonica OX=399... [more]
P0C0X51.4e-8137.00Probable auxin efflux carrier component 1b OS=Oryza sativa subsp. japonica OX=39... [more]
Q0IQA51.5e-8036.38Probable auxin efflux carrier component 1d OS=Oryza sativa subsp. japonica OX=39... [more]
Q67UL36.7e-7634.80Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39... [more]
Match NameE-valueIdentityDescription
XP_011652244.11.38e-236100.00auxin efflux carrier component 8 isoform X2 [Cucumis sativus] >KGN59594.1 hypoth... [more]
XP_031738517.19.65e-23599.72auxin efflux carrier component 8 isoform X1 [Cucumis sativus][more]
XP_008443438.11.41e-22997.19PREDICTED: putative auxin efflux carrier component 5 [Cucumis melo] >TYK25662.1 ... [more]
XP_038904643.14.04e-22996.35auxin efflux carrier component 8 [Benincasa hispida][more]
KAA0053741.19.86e-22896.92putative auxin efflux carrier component 5 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A0A0LFB76.68e-237100.00Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_3G827360 PE=3 S... [more]
A0A5D3DQ586.83e-23097.19Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3B8S26.83e-23097.19Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103487029 PE=3 SV=1[more]
A0A5A7UJS24.77e-22896.92Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A6J1J3G01.34e-22092.98Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111482319 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT5G15100.11.6e-10958.31Auxin efflux carrier family protein [more]
AT2G01420.12.2e-7432.19Auxin efflux carrier family protein [more]
AT2G01420.26.4e-7431.98Auxin efflux carrier family protein [more]
AT1G23080.34.2e-7333.33Auxin efflux carrier family protein [more]
AT1G23080.11.2e-7233.12Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014024Auxin efflux carrier, plant typeTIGRFAMTIGR00946TIGR00946coord: 1..356
e-value: 2.8E-99
score: 330.3
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 9..351
e-value: 2.0E-100
score: 335.9
NoneNo IPR availablePANTHERPTHR31752AUXIN EFFLUX CARRIER COMPONENT 1B-RELATEDcoord: 1..355
NoneNo IPR availablePANTHERPTHR31752:SF40AUXIN EFFLUX CARRIER COMPONENT 8coord: 1..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G036840.1CsGy3G036840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0009926 auxin polar transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity