CsGy3G030560 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G030560
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Ribonuclease T(2)
Location	Gy14Chr3: 30534073 .. 30534875 (+)
RNA-Seq Expression	CsGy3G030560
Synteny	CsGy3G030560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCAACATATTTTCATTTTATGTCTTTTAAGTTCGTTGTTGTTTGTAAATGGCATTCCATTCGACTTCTTCCAACTAGTTCAACAATGGGGTCCAAACATATGTAGTCCTGCTGGCAGCAAGTGTTATGCTCAACCTCAACCCATGTTCACTATTCATGGGCTATGGCCAAGTAACTTTTCGACTGTCCCAATTTTGTGTCCAAGGACTAAAAAACTATTTCCACAGGGAAAGGTTCCTATCTTCTTGATTTTTTTTTCTGTGCTTTTTTTTTTTTTTCTATCTTTAGCAATCTATTCATTGTTTCAACACAATCTTTTATTCTATTTTAGGTTACATCACTTGAACAACATCTAAATAAGTACTGGCCAGATGTAATAAGTGGAAAGAACTCCAACTTCTGGGAACATGAATGGAGAAAGCACGCGGAATGTATAGACCCTCCTTTTACCATATTTTGGTATTTCGATATTTCTTTGAGACACCGAATGAATAAAACGTATGATCTGTTAACAATCTTAAATGATGCTGGACTCAGTCACCAAACGCACAAAGATCTCATATCTCAAAACGTTCTAGACCCCATTAAAAATGCAACAGGAATGGAACCAGGCATCCGTTGCAACATAAATGGTATAACTAAGAAGTTGCAATTGAAAGAAATTGTATTGTGTTTTGGCAATGATGGTACTACCCTCATAGATTGCCCTTCGTTTGTTAGCAACACTTGTACAAGTCAACCTAAATTTGTGTGGCTCCTCCCACAACAGAGTTCAGTAGGTGTTCCAGATTATCCATGA

mRNA sequence

ATGGCTCAACATATTTTCATTTTATGTCTTTTAAGTTCGTTGTTGTTTGTAAATGGCATTCCATTCGACTTCTTCCAACTAGTTCAACAATGGGGTCCAAACATATGTAGTCCTGCTGGCAGCAAGTGTTATGCTCAACCTCAACCCATGTTCACTATTCATGGGCTATGGCCAAGTAACTTTTCGACTGTCCCAATTTTGTGTCCAAGGACTAAAAAACTATTTCCACAGGGAAAGGTTACATCACTTGAACAACATCTAAATAAGTACTGGCCAGATGTAATAAGTGGAAAGAACTCCAACTTCTGGGAACATGAATGGAGAAAGCACGCGGAATGTATAGACCCTCCTTTTACCATATTTTGGTATTTCGATATTTCTTTGAGACACCGAATGAATAAAACGTATGATCTGTTAACAATCTTAAATGATGCTGGACTCAGTCACCAAACGCACAAAGATCTCATATCTCAAAACGTTCTAGACCCCATTAAAAATGCAACAGGAATGGAACCAGGCATCCGTTGCAACATAAATGGTATAACTAAGAAGTTGCAATTGAAAGAAATTGTATTGTGTTTTGGCAATGATGGTACTACCCTCATAGATTGCCCTTCGTTTGTTAGCAACACTTGTACAAGTCAACCTAAATTTGTGTGGCTCCTCCCACAACAGAGTTCAGTAGGTGTTCCAGATTATCCATGA

Coding sequence (CDS)

ATGGCTCAACATATTTTCATTTTATGTCTTTTAAGTTCGTTGTTGTTTGTAAATGGCATTCCATTCGACTTCTTCCAACTAGTTCAACAATGGGGTCCAAACATATGTAGTCCTGCTGGCAGCAAGTGTTATGCTCAACCTCAACCCATGTTCACTATTCATGGGCTATGGCCAAGTAACTTTTCGACTGTCCCAATTTTGTGTCCAAGGACTAAAAAACTATTTCCACAGGGAAAGGTTACATCACTTGAACAACATCTAAATAAGTACTGGCCAGATGTAATAAGTGGAAAGAACTCCAACTTCTGGGAACATGAATGGAGAAAGCACGCGGAATGTATAGACCCTCCTTTTACCATATTTTGGTATTTCGATATTTCTTTGAGACACCGAATGAATAAAACGTATGATCTGTTAACAATCTTAAATGATGCTGGACTCAGTCACCAAACGCACAAAGATCTCATATCTCAAAACGTTCTAGACCCCATTAAAAATGCAACAGGAATGGAACCAGGCATCCGTTGCAACATAAATGGTATAACTAAGAAGTTGCAATTGAAAGAAATTGTATTGTGTTTTGGCAATGATGGTACTACCCTCATAGATTGCCCTTCGTTTGTTAGCAACACTTGTACAAGTCAACCTAAATTTGTGTGGCTCCTCCCACAACAGAGTTCAGTAGGTGTTCCAGATTATCCATGA

Protein sequence

MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSNFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPPFTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNINGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP*

Homology

[Close]

BLAST of CsGy3G030560 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 5.8e-28
Identity = 70/186 (37.63%), Postives = 93/186 (50.00%), Query Frame = 0


Query: 22  FDFFQLVQQWGPNICS-PAGSKCYAQPQPMFTIHGLWPSNFSTVPILCPRTKKLFPQGKV 81
           FD F  VQQW P +CS      C       FTIHGLWP    T    CP+    F   K+
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSP-FDITKI 60

Query: 82  TSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPPFTIFWYFDISLRHRMNKTYDLLT 141
           + L+  LN  WP+V+   N  FW HEW KH  C +  F    YF +++  R N  YD++ 
Sbjct: 61  SHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNN--YDIIG 120

Query: 142 IL--NDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNINGITKKLQLKEIVLCFGNDG 201
            L  + AG + +T      Q +   +K   G  PG+RC  +  TK   L ++V CF  DG
Sbjct: 121 ALRPHAAGPNGRTKS---RQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDG 180

Query: 202 TTLIDC 205
           +TLIDC
Sbjct: 181 STLIDC 180

[Close]

BLAST of CsGy3G030560 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 9.3e-26
Identity = 73/225 (32.44%), Postives = 106/225 (47.11%), Query Frame = 0


Query: 8   LCLLSSLLFVNGI--------PFDFFQLVQQWGPNICSPAGSKCY---AQPQPMFTIHGL 67
           LCL+S L+ +  +         FDFF  VQQW  + C      CY    +P   F IHGL
Sbjct: 8   LCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGL 67

Query: 68  WPS-NFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVI--SGKNSNFWEHEWRKHAEC 127
           WP+    T P  C  +K  F    ++ L   + K WP +   SG    FWEHEW KH  C
Sbjct: 68  WPNYKDGTYPSNCDASKP-FDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTC 127

Query: 128 IDPPFTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPG 187
            +       YF  +L   + +  +LL  L  AG+ +   K    +++ D IK + G  P 
Sbjct: 128 SESVIDQHEYFQTAL--NLKQKTNLLGALTKAGI-NPDGKSYSLESIRDSIKESIGFTPW 187

Query: 188 IRCNINGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKF 219
           + CN +G +   QL ++ LC    G+ LI+CP F    C ++ +F
Sbjct: 188 VECNRDG-SGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEF 227

[Close]

BLAST of CsGy3G030560 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 1.0e-24
Identity = 73/222 (32.88%), Postives = 108/222 (48.65%), Query Frame = 0


Query: 5   IFILCLLSSLLFVNGI-PFDFFQLVQQWGPNICSPAGSKCY---AQPQPMFTIHGLWP-S 64
           + +L +   L  +N    FDFF  VQQW  + C    S CY    +P   F IHGLWP +
Sbjct: 11  LILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWPNN 70

Query: 65  NFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVI--SGKNSNFWEHEWRKHAECIDPP 124
           N  T P  C      + Q +++ L   + + WP +   SG  S FW HEW KH  C +  
Sbjct: 71  NDGTYPSNCDPNSP-YDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESV 130

Query: 125 FT-IFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRC 184
            T    YF  +L   +    DLL+IL  A + H   +     N+ + IK+A G  P I+C
Sbjct: 131 LTNQHAYFKKAL--DLKNQIDLLSILQGADI-HPDGESYDLVNIRNAIKSAIGYTPWIQC 190

Query: 185 NINGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKF 219
           N++  +   QL ++ +C    G++LI+CP F    C +  +F
Sbjct: 191 NVDQ-SGNSQLYQVYICVDGSGSSLIECPIFPGGKCGTSIEF 227

[Close]

BLAST of CsGy3G030560 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.9e-21
Identity = 72/221 (32.58%), Postives = 98/221 (44.34%), Query Frame = 0


Query: 5   IFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQ---PQPMFTIHGLWPSNF 64
           IFIL L    +      FDFF  V QW    C    S CY Q   P   F IHGLWP N+
Sbjct: 5   IFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWP-NY 64

Query: 65  ST--VPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNS--NFWEHEWRKHAECIDPP 124
            T   P  C    + F   +V+ L   L + WP +    N    FW HEW KH  C +  
Sbjct: 65  KTGGWPQNCNPDSR-FDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESE 124

Query: 125 FTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN 184
                YF+  L  ++ +  +LL  L +AG+     K    +++ + IK   G  PGI CN
Sbjct: 125 LDQHDYFEAGL--KLKQKANLLHALTNAGIK-PDDKFYEMKDIENTIKQVVGFAPGIECN 184

Query: 185 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKF 219
            +  +   QL +I LC     +  I+CP      C S+ +F
Sbjct: 185 KDS-SHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQF 219

[Close]

BLAST of CsGy3G030560 vs. ExPASy Swiss-Prot
Match: O80323 (Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.3e-19
Identity = 66/205 (32.20%), Postives = 104/205 (50.73%), Query Frame = 0


Query: 5   IFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSNF-ST 64
           +F+L +L  +L  + + +D+FQ  QQ+   +C+   + C   P  +FT+HGLWPSN    
Sbjct: 8   VFLLIVL--ILCSSTVGYDYFQFTQQYQLAVCNSNRTLCKDPPDKLFTVHGLWPSNMVGP 67

Query: 65  VPILCPRTKKLFPQGKVTSLEQHLNKYWPDVIS-GKNSNFWEHEWRKHAECIDPPF-TIF 124
            P  CP  K +  + K+  LE  L   WP+V    KN+ FW+ EW KH  C  P      
Sbjct: 68  DPSKCP-IKNIRKREKL--LEHQLEIIWPNVFDRTKNNLFWDKEWMKHGSCGYPTIDNEN 127

Query: 125 WYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKN-ATGMEPGIRCNING 184
            YF+  ++  ++K  ++  IL+ A +     K  +  ++ + I+N A   +P ++C   G
Sbjct: 128 HYFETVIKMYISKKQNVSRILSKAKIEPDGKKRAL-LDIENAIRNGADNKKPKLKCQKKG 187

Query: 185 ITKKLQLKEIVLCFGNDGTTLIDCP 206
            T   +L EI LC    G   IDCP
Sbjct: 188 TT--TELVEITLCSDKSGEHFIDCP 204

[Close]

BLAST of CsGy3G030560 vs. NCBI nr
Match: XP_011651927.1 (ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [Cucumis sativus])

HSP 1 Score: 502 bits (1292), Expect = 1.02e-179
Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSN 60
           MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSN
Sbjct: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSN 60

Query: 61  FSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPPFTI 120
           FSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHA+CIDPPFTI
Sbjct: 61  FSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFTI 120

Query: 121 FWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNING 180
           FWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNING
Sbjct: 121 FWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNING 180

Query: 181 ITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234
           ITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP
Sbjct: 181 ITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234

[Close]

BLAST of CsGy3G030560 vs. NCBI nr
Match: TYK25090.1 (S-RNase [Cucumis melo var. makuwa])

HSP 1 Score: 311 bits (797), Expect = 2.41e-104
Identity = 154/237 (64.98%), Postives = 182/237 (76.79%), Query Frame = 0


Query: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSN 60
           MAQH FILCLLS LLFVNG PFDFFQLV+QWGPN C+  G++C+AQPQP FTIHGLWPSN
Sbjct: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCND-GTQCHAQPQPEFTIHGLWPSN 60

Query: 61  FSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPPFTI 120
           FS   + C  T+KLF  G+++ LE  L++YWPDVI+G N  FW +EW+KH  CIDPPF I
Sbjct: 61  FSNARLFCA-TRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNI 120

Query: 121 FWYFDISLRHRMNKTYDLLTILNDAGLSHQTHK----DLISQNVLDPIKNATGMEPGIRC 180
             YF +SL H  ++TYDLL ILN  G+ H+  +     L  +++L+PI NAT    GIRC
Sbjct: 121 TQYFQLSLDH--HRTYDLLKILN--GVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRC 180

Query: 181 NINGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDY 233
           N NGITKKLQL EIVLCF N+GTTLIDCP FVSN+C +QPKFVWLLPQQSS GVPDY
Sbjct: 181 NKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSC-NQPKFVWLLPQQSSDGVPDY 230

[Close]

BLAST of CsGy3G030560 vs. NCBI nr
Match: XP_031738690.1 (ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 231 bits (590), Expect = 4.09e-73
Identity = 119/223 (53.36%), Postives = 147/223 (65.92%), Query Frame = 0


Query: 1   MAQHIFILCLLSSLLFVNG-IPFDFFQLVQQWGPNICSPAG--SKCYAQPQPMFTIHGLW 60
           M Q I I+  LS ++FVNG +PF+FFQ V+QWGPN+C+     S C+  PQ  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPP 120
           PSN S   +LC ++K ++   KV SLE  LN  WPD+  G N  FW HEW  H +C DPP
Sbjct: 61  PSNHSNAHLLC-QSKTIY--SKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPP 120

Query: 121 FTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN 180
           F++F YF ISL   + + +DLLTIL  AGL+ QT ++L  QN++ PI+  T   PGIRCN
Sbjct: 121 FSLFQYFQISLN--LLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCN 180

Query: 181 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVW 220
            N  T K QL EIVLC   DG TLIDCP+FVSNTC     FVW
Sbjct: 181 KNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAK--SFVW 216

[Close]

BLAST of CsGy3G030560 vs. NCBI nr
Match: XP_031738547.1 (ribonuclease MC-like [Cucumis sativus] >XP_031739193.1 ribonuclease MC-like [Cucumis sativus] >KAE8651059.1 hypothetical protein Csa_001394 [Cucumis sativus] >KAE8651065.1 hypothetical protein Csa_001488 [Cucumis sativus])

HSP 1 Score: 217 bits (553), Expect = 1.67e-67
Identity = 111/223 (49.78%), Postives = 141/223 (63.23%), Query Frame = 0


Query: 1   MAQHIFILCLLSSLLFVNG-IPFDFFQLVQQWGPNICSPAG--SKCYAQPQPMFTIHGLW 60
           M Q I I+  LS ++FVNG +PF+FFQ V+QWGPN+C+     S C+  PQ  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPP 120
           PSN S   +LC    K     KV  LE+ LN  WPD+  G N+ FW HEW  H +C DPP
Sbjct: 61  PSNHSNAHLLCQSITKYT---KVLLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPP 120

Query: 121 FTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN 180
           F++F YF +SL   + + +D+L IL  AGL+ QT +++  QN++ PI+  T   PGIRC 
Sbjct: 121 FSLFQYFQMSLN--LLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCY 180

Query: 181 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVW 220
            N  T K QL EIVLC   DG TLIDCP+FVS+TC      VW
Sbjct: 181 KNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAK--SIVW 216

[Close]

BLAST of CsGy3G030560 vs. NCBI nr
Match: KAE8651058.1 (hypothetical protein Csa_002465 [Cucumis sativus])

HSP 1 Score: 231 bits (590), Expect = 1.68e-67
Identity = 119/223 (53.36%), Postives = 147/223 (65.92%), Query Frame = 0


Query: 1   MAQHIFILCLLSSLLFVNG-IPFDFFQLVQQWGPNICSPAG--SKCYAQPQPMFTIHGLW 60
           M Q I I+  LS ++FVNG +PF+FFQ V+QWGPN+C+     S C+  PQ  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPP 120
           PSN S   +LC ++K ++   KV SLE  LN  WPD+  G N  FW HEW  H +C DPP
Sbjct: 61  PSNHSNAHLLC-QSKTIY--SKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPP 120

Query: 121 FTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN 180
           F++F YF ISL   + + +DLLTIL  AGL+ QT ++L  QN++ PI+  T   PGIRCN
Sbjct: 121 FSLFQYFQISLN--LLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCN 180

Query: 181 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVW 220
            N  T K QL EIVLC   DG TLIDCP+FVSNTC     FVW
Sbjct: 181 KNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAK--SFVW 216

[Close]

BLAST of CsGy3G030560 vs. ExPASy TrEMBL
Match: A0A0A0LAB0 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1)

HSP 1 Score: 502 bits (1292), Expect = 4.93e-180
Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSN 60
           MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSN
Sbjct: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSN 60

Query: 61  FSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPPFTI 120
           FSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHA+CIDPPFTI
Sbjct: 61  FSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFTI 120

Query: 121 FWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNING 180
           FWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNING
Sbjct: 121 FWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNING 180

Query: 181 ITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234
           ITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP
Sbjct: 181 ITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234

[Close]

BLAST of CsGy3G030560 vs. ExPASy TrEMBL
Match: A0A5D3DP91 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002140 PE=3 SV=1)

HSP 1 Score: 311 bits (797), Expect = 1.17e-104
Identity = 154/237 (64.98%), Postives = 182/237 (76.79%), Query Frame = 0


Query: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSN 60
           MAQH FILCLLS LLFVNG PFDFFQLV+QWGPN C+  G++C+AQPQP FTIHGLWPSN
Sbjct: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCND-GTQCHAQPQPEFTIHGLWPSN 60

Query: 61  FSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPPFTI 120
           FS   + C  T+KLF  G+++ LE  L++YWPDVI+G N  FW +EW+KH  CIDPPF I
Sbjct: 61  FSNARLFCA-TRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNI 120

Query: 121 FWYFDISLRHRMNKTYDLLTILNDAGLSHQTHK----DLISQNVLDPIKNATGMEPGIRC 180
             YF +SL H  ++TYDLL ILN  G+ H+  +     L  +++L+PI NAT    GIRC
Sbjct: 121 TQYFQLSLDH--HRTYDLLKILN--GVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRC 180

Query: 181 NINGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDY 233
           N NGITKKLQL EIVLCF N+GTTLIDCP FVSN+C +QPKFVWLLPQQSS GVPDY
Sbjct: 181 NKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSC-NQPKFVWLLPQQSSDGVPDY 230

[Close]

BLAST of CsGy3G030560 vs. ExPASy TrEMBL
Match: A0A0A0LAN6 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 231 bits (590), Expect = 1.98e-73
Identity = 119/223 (53.36%), Postives = 147/223 (65.92%), Query Frame = 0


Query: 1   MAQHIFILCLLSSLLFVNG-IPFDFFQLVQQWGPNICSPAG--SKCYAQPQPMFTIHGLW 60
           M Q I I+  LS ++FVNG +PF+FFQ V+QWGPN+C+     S C+  PQ  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPP 120
           PSN S   +LC ++K ++   KV SLE  LN  WPD+  G N  FW HEW  H +C DPP
Sbjct: 61  PSNHSNAHLLC-QSKTIY--SKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPP 120

Query: 121 FTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN 180
           F++F YF ISL   + + +DLLTIL  AGL+ QT ++L  QN++ PI+  T   PGIRCN
Sbjct: 121 FSLFQYFQISLN--LLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCN 180

Query: 181 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVW 220
            N  T K QL EIVLC   DG TLIDCP+FVSNTC     FVW
Sbjct: 181 KNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAK--SFVW 216

[Close]

BLAST of CsGy3G030560 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 213 bits (543), Expect = 1.53e-65
Identity = 110/223 (49.33%), Postives = 134/223 (60.09%), Query Frame = 0


Query: 1   MAQHIFILCLLSSLLFVNGIP--FDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWP 60
           MAQ  FI+ +   +L  + +   FD+FQ+VQQW P  CS  G +CYA P  MFTIHGLWP
Sbjct: 1   MAQAPFIVSIFKFVLLTSCVKAQFDYFQMVQQWPPATCS--GVRCYANPPAMFTIHGLWP 60

Query: 61  SNFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPPF 120
           SN+S VP++C    + F   ++ +L   LN YWPDVISG N  FW+HEW  H  C DPPF
Sbjct: 61  SNYSNVPLVC--AGQPFNPAQIATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPF 120

Query: 121 TIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNI 180
               YF  +L  R N  YDLL ILN AGL            +   I+ ATG +PG+RCN 
Sbjct: 121 NQLQYFQTTLNIRTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRCNK 180

Query: 181 NGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWL 221
           N  +KK QL EI+LCF  +G TLIDC  F   TC SQ  FVWL
Sbjct: 181 NAQSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQ--FVWL 217

[Close]

BLAST of CsGy3G030560 vs. ExPASy TrEMBL
Match: A0A6J1IAR7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1)

HSP 1 Score: 210 bits (534), Expect = 3.95e-64
Identity = 106/218 (48.62%), Postives = 133/218 (61.01%), Query Frame = 0


Query: 6   FILCLLSSLLFVNGIP--FDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSNFST 65
           FI+ + + +L   G+   FD+FQ+VQQW P  CS  G +CYA P  MFTIHGLWPS +S 
Sbjct: 7   FIVSIFNLVLLTIGVKAQFDYFQMVQQWPPATCS--GVRCYANPPAMFTIHGLWPSTYSN 66

Query: 66  VPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHAECIDPPFTIFWY 125
           VP +C    + F + ++ +LE  LN YWPDVI+G N  FW+HEW  H  C DPPF    Y
Sbjct: 67  VPPVC--AGQPFDRAQIATLEPQLNTYWPDVINGNNQRFWKHEWDNHGTCSDPPFNQLQY 126

Query: 126 FDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNINGITK 185
           F  +L  R N  YDLL ILN AGL          Q +   I+ ATG +PG+RCN N  +K
Sbjct: 127 FQTTLNIRTNHNYDLLAILNTAGLGPTATTTRQYQAIEGAIQAATGKKPGLRCNKNAQSK 186

Query: 186 KLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWL 221
           + QL EI+LCF  +G TLI+C  F   TC SQ  FVWL
Sbjct: 187 RKQLFEIILCFDKNGVTLINCTQFAGITCPSQ--FVWL 218

[Close]

BLAST of CsGy3G030560 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 118.6 bits (296), Expect = 6.6e-27
Identity = 73/225 (32.44%), Postives = 106/225 (47.11%), Query Frame = 0


Query: 8   LCLLSSLLFVNGI--------PFDFFQLVQQWGPNICSPAGSKCY---AQPQPMFTIHGL 67
           LCL+S L+ +  +         FDFF  VQQW  + C      CY    +P   F IHGL
Sbjct: 8   LCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGL 67

Query: 68  WPS-NFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVI--SGKNSNFWEHEWRKHAEC 127
           WP+    T P  C  +K  F    ++ L   + K WP +   SG    FWEHEW KH  C
Sbjct: 68  WPNYKDGTYPSNCDASKP-FDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTC 127

Query: 128 IDPPFTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPG 187
            +       YF  +L   + +  +LL  L  AG+ +   K    +++ D IK + G  P 
Sbjct: 128 SESVIDQHEYFQTAL--NLKQKTNLLGALTKAGI-NPDGKSYSLESIRDSIKESIGFTPW 187

Query: 188 IRCNINGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKF 219
           + CN +G +   QL ++ LC    G+ LI+CP F    C ++ +F
Sbjct: 188 VECNRDG-SGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEF 227

[Close]

BLAST of CsGy3G030560 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 114.8 bits (286), Expect = 9.6e-26
Identity = 67/203 (33.00%), Postives = 101/203 (49.75%), Query Frame = 0


Query: 22  FDFFQLVQQWGPNICSPAGSKCY---AQPQPMFTIHGLWPS-NFSTVPILCPRTKKLFPQ 81
           FDFF  V QW    C  + + CY    +P   F IHGLWP+ N  + P  C    + F +
Sbjct: 28  FDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQ-FDR 87

Query: 82  GKVTSLEQHLNKYWPDVI--SGKNSNFWEHEWRKHAECIDPPFTIFWYFDISLRHRMNKT 141
            +++ L   L K WP +   S +  NFWEHEW KH  C +       YF+ +L  ++ + 
Sbjct: 88  SQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENAL--KLKQK 147

Query: 142 YDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNINGITKKLQLKEIVLCFG 201
            +LL IL ++G++       + + + + IK+  G  PGI CN     +  QL +I +C  
Sbjct: 148 ANLLQILKNSGINPDDGFYNLDK-ITNAIKDGIGFTPGIECN-KDPERNAQLHQIYICVD 207

Query: 202 NDGTTLIDCPSFVSNTCTSQPKF 219
             GT  I+CP     +C SQ +F
Sbjct: 208 TSGTEFIECPVLPRGSCPSQIQF 225

[Close]

BLAST of CsGy3G030560 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 102.4 bits (254), Expect = 4.9e-22
Identity = 72/221 (32.58%), Postives = 98/221 (44.34%), Query Frame = 0


Query: 5   IFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQ---PQPMFTIHGLWPSNF 64
           IFIL L    +      FDFF  V QW    C    S CY Q   P   F IHGLWP N+
Sbjct: 5   IFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWP-NY 64

Query: 65  ST--VPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNS--NFWEHEWRKHAECIDPP 124
            T   P  C    + F   +V+ L   L + WP +    N    FW HEW KH  C +  
Sbjct: 65  KTGGWPQNCNPDSR-FDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESE 124

Query: 125 FTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN 184
                YF+  L  ++ +  +LL  L +AG+     K    +++ + IK   G  PGI CN
Sbjct: 125 LDQHDYFEAGL--KLKQKANLLHALTNAGIK-PDDKFYEMKDIENTIKQVVGFAPGIECN 184

Query: 185 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKF 219
            +  +   QL +I LC     +  I+CP      C S+ +F
Sbjct: 185 KDS-SHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQF 219

[Close]

BLAST of CsGy3G030560 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 101.7 bits (252), Expect = 8.4e-22
Identity = 68/215 (31.63%), Postives = 96/215 (44.65%), Query Frame = 0


Query: 22  FDFFQLVQQWGPNICSPAGSKC---YAQPQPMFTIHGLWPS-NFSTVPILCPRTKKLFPQ 81
           F+FF  V  W   IC      C          F IHGLWP  N  T P  C +T  LF  
Sbjct: 26  FNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLWPQFNNGTWPAFCDQT-NLFDI 85

Query: 82  GKVTSLEQHLNKYWPD----VISGKNSNFWEHEWRKHAECIDPPFTIFWYFDISLRHRMN 141
            K++ L   + K W +          +N WEHEW KH  C+   F    YF  +L+ + +
Sbjct: 86  SKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTCVQSIFDQHSYFRTNLKFK-H 145

Query: 142 KTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNINGITKKLQLKEIVLC 201
           K + LL IL   G+        + + + + IK A G  PGI CN   +    QL +I +C
Sbjct: 146 KVH-LLNILIQKGIKPNDGFYSLDE-IKNAIKCAIGFAPGIECN-EDVKGNKQLFQIYIC 205

Query: 202 FGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSV 229
             N     ++CP     +C S+ KF   LP++ S+
Sbjct: 206 LDNYAKEFVECPYVPDKSCASKIKFP-SLPERDSL 234

The following BLAST results are available for this feature:

BLAST of CsGy3G030560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	5.8e-28	37.63	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	9.3e-26	32.44	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80022	1.0e-24	32.88	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42815	6.9e-21	32.58	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
O80323	1.3e-19	32.20	Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]

BLAST of CsGy3G030560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651927.1	1.02e-179	99.57	ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [C...	[more]
TYK25090.1	2.41e-104	64.98	S-RNase [Cucumis melo var. makuwa]	[more]
XP_031738690.1	4.09e-73	53.36	ribonuclease MC-like [Cucumis sativus]	[more]
XP_031738547.1	1.67e-67	49.78	ribonuclease MC-like [Cucumis sativus] >XP_031739193.1 ribonuclease MC-like [Cuc...	[more]
KAE8651058.1	1.68e-67	53.36	hypothetical protein Csa_002465 [Cucumis sativus]	[more]

BLAST of CsGy3G030560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAB0	4.93e-180	99.57	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1	[more]
A0A5D3DP91	1.17e-104	64.98	Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00...	[more]
A0A0A0LAN6	1.98e-73	53.36	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1	[more]
A0A6J1EXD5	1.53e-65	49.33	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1	[more]
A0A6J1IAR7	3.95e-64	48.62	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1	[more]

BLAST of CsGy3G030560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	6.6e-27	32.44	ribonuclease 1	[more]
AT1G14220.1	9.6e-26	33.00	Ribonuclease T2 family protein	[more]
AT1G26820.1	4.9e-22	32.58	ribonuclease 3	[more]
AT1G14210.1	8.4e-22	31.63	Ribonuclease T2 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 20..220 e-value: 2.6E-45 score: 157.0
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 22..219
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 22..204 e-value: 1.0E-34 score: 120.3
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 10..215
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 10..215
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 51..58
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 102..113
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 22..218 e-value: 5.11997E-50 score: 160.19

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy3G030560.1	CsGy3G030560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding