CsGy3G023650 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G023650
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationGy14Chr3: 23501521 .. 23503905 (+)
RNA-Seq ExpressionCsGy3G023650
SyntenyCsGy3G023650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAATTGATTGTTTAGCAAAATTAGAAGTTCCTATTATAAAATTGTCCAACGTTGATAAAAAAGATGATGATAGTGATCCTGAAAGCAGAAAATTATGGTTAGAAAGAAGCAAGCAAGTGAGAGATGCTCTTGAAGAATATGGGTGTTTTTTAGGTGAGTATGACGATGATGAGAGTAGCTGCAGTTGTAATTCATCTTCATCACCAAAGCTTAATAGTAATAATTATGAAGAAATTTTCAAGGGATTGAAAGAGCTTTTTGATTTGCCATTAGAAACTAAAATAAAGAATGTTTCTGATAAGCCATACCATGGATTTTTGGACTGTAGAACTCCCTTTGTTCTTCCTCTTCATCAAAGCCTCGGTATTGAAAATGCTTCATCTTACGAAGCTGTTCAGTTTTTTGTCAACCTCTTATGGCCTTCTGGCAATCATCACTTCTGGTAATTATTACTTATGGTATTTGATATTCTTATATGTTTTTTCTTTCTATATAATCTTTGATTTTTTTCTTGTTTTTTTTTTTTTTTAATTGTTCTTCATCCCATTGATTCATTCATCATATAATATATATTTAAGTTCAATTGTTCTTGTTAAATTAAAGTTCTCTATTTTGGGACTAAATTTATATATATATAAAAAAAAAAAACTCTCTTGATTTTGCTGATCAAAATATTAATTTCTAGCCGTGTGTAAATTATGTTGTTCTTATTTATTTATCATAGAATCTTTACTCTTTTACTGTTTTTCCAGCTAATGTTAGAGTATATTTACATCTTTAATAATTTTTAAACTAAATATACACTCATGATTTTCTATTCTGGAAAATCAATTAAGATCATGTTTAATAACCATTATTTTAAAGTTTGCTTTTAATTTTTTTTTCAAAATTTATTCTTGATTTCTTTCAAATTCTTAACCATGTGATCATTTTCAATTGATACTAAAATAAATATTTAAATTCTTAATCAAAATTTAAAAACAAAAATTAGTTTTTAAAATCTAATTTTTGAGTTTTTCAAAGTGTTGCCAGTAGAAAGTAGACTAAACAATACAGATCAGTGAAGTGAAAGTAGGGTTTGAAAGTTGAATTTTCAAAACAAAACACAATAAAAAGACAAAAAAATAGAAAGGAAAAAAAAGAGTTAATATATATATTTGTAAAGAATTTCTCATATGCCATTTTGTAAAATTTTCTTTTATTCCTATGTGGGTTTATTGAATTTGTACATAAATGAAAATAAATTTGTATTTGGACAGTGAAAAATTATTTGCATACTCAAAGAAGATATCAGAGATGGAGCAATTAGTAAAGAGGATGGTATTTGAAAGCTATGGAGTGGGAAAATACTATGATTCTCATATTGCTTCAACCACTTATCTCCTAAGAACAATGAAATATAGAATTCCAAAAATGGGTGAAGAGAATATTGGTGCAGAAGCTCACACTGACAAAAGTTTCTTCACTATTCTTCATCAATTTGATGGAGTCAATGGCTTGCAAATCAAACCAAAAAATTATGATCAATGGCTTGGAGTTCAATTCTCTCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGTAATTAAATAATATCTTCTTTTTTCTTTTCTTTTCTTTTTTTATATATCTATTTTAATAACATTTTTTTTAGGCTTCAATTCTTTTCTTATGGTTGAATGATATAATATTAAACATTATTTTAAACTTTTGAGTTAATCAGTTATTTAAGACAATATCTGAGTAAGTTGTCCAAAGAGATCATGTATTTAGGCTCCGACAATGTTATTTCCTCTCTCATTAAAATTGATTTTCATTTATTGGGGTCTTCTCATATTAAACCCACAAGTGAAGATAGTGTTGAGTGATATAATATTATTATTAGTTAAATTTATCTTCAGTTACTAGATTAAGTTTTTGGATCAACAGTTACTGACTGCATTAACCAAATTACACTACAAGAAGTTTAAATGTTCAAAACATCAAATCAAATAAAAAGTCAATATAAACAAGTTGAAAGTGTATCATGCAGAATGTTTCCTAATTCAATTCATATTTTGTTTGGATGGTAAGAATTGATAAAGTTGAATCTCATCAATAATATATATATACATTGTTATTTTATGAATTAGGCATGGAGCAATGGGAGAATTCACTCAGCCACTCATAGAGTTATAATTGAGGGAAACAAAGAGAGATATTCAACTGGCCTATTTTCATACCACAAAGGAATTATTGAAATTCCACAAGAATTGGTGGATGAGAAACATCCCTTGAGATTTAACCCTTTTAACCATTATGGATTACTTGGTTATTTTTCCACTGATGATTGTGACAAAATCCCTTCAACTGCCAATGCTTATTGTGGAGTTTGA

mRNA sequence

ATGGGAATTGATTGTTTAGCAAAATTAGAAGTTCCTATTATAAAATTGTCCAACGTTGATAAAAAAGATGATGATAGTGATCCTGAAAGCAGAAAATTATGGTTAGAAAGAAGCAAGCAAGTGAGAGATGCTCTTGAAGAATATGGGTGTTTTTTAGGTGAGTATGACGATGATGAGAGTAGCTGCAGTTGTAATTCATCTTCATCACCAAAGCTTAATAGTAATAATTATGAAGAAATTTTCAAGGGATTGAAAGAGCTTTTTGATTTGCCATTAGAAACTAAAATAAAGAATGTTTCTGATAAGCCATACCATGGATTTTTGGACTGTAGAACTCCCTTTGTTCTTCCTCTTCATCAAAGCCTCGGTATTGAAAATGCTTCATCTTACGAAGCTGTTCAGTTTTTTGTCAACCTCTTATGGCCTTCTGGCAATCATCACTTCTGTGAAAAATTATTTGCATACTCAAAGAAGATATCAGAGATGGAGCAATTAGTAAAGAGGATGGTATTTGAAAGCTATGGAGTGGGAAAATACTATGATTCTCATATTGCTTCAACCACTTATCTCCTAAGAACAATGAAATATAGAATTCCAAAAATGGGTGAAGAGAATATTGGTGCAGAAGCTCACACTGACAAAAGTTTCTTCACTATTCTTCATCAATTTGATGGAGTCAATGGCTTGCAAATCAAACCAAAAAATTATGATCAATGGCTTGGAGTTCAATTCTCTCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGCATGGAGCAATGGGAGAATTCACTCAGCCACTCATAGAGTTATAATTGAGGGAAACAAAGAGAGATATTCAACTGGCCTATTTTCATACCACAAAGGAATTATTGAAATTCCACAAGAATTGGTGGATGAGAAACATCCCTTGAGATTTAACCCTTTTAACCATTATGGATTACTTGGTTATTTTTCCACTGATGATTGTGACAAAATCCCTTCAACTGCCAATGCTTATTGTGGAGTTTGA

Coding sequence (CDS)

ATGGGAATTGATTGTTTAGCAAAATTAGAAGTTCCTATTATAAAATTGTCCAACGTTGATAAAAAAGATGATGATAGTGATCCTGAAAGCAGAAAATTATGGTTAGAAAGAAGCAAGCAAGTGAGAGATGCTCTTGAAGAATATGGGTGTTTTTTAGGTGAGTATGACGATGATGAGAGTAGCTGCAGTTGTAATTCATCTTCATCACCAAAGCTTAATAGTAATAATTATGAAGAAATTTTCAAGGGATTGAAAGAGCTTTTTGATTTGCCATTAGAAACTAAAATAAAGAATGTTTCTGATAAGCCATACCATGGATTTTTGGACTGTAGAACTCCCTTTGTTCTTCCTCTTCATCAAAGCCTCGGTATTGAAAATGCTTCATCTTACGAAGCTGTTCAGTTTTTTGTCAACCTCTTATGGCCTTCTGGCAATCATCACTTCTGTGAAAAATTATTTGCATACTCAAAGAAGATATCAGAGATGGAGCAATTAGTAAAGAGGATGGTATTTGAAAGCTATGGAGTGGGAAAATACTATGATTCTCATATTGCTTCAACCACTTATCTCCTAAGAACAATGAAATATAGAATTCCAAAAATGGGTGAAGAGAATATTGGTGCAGAAGCTCACACTGACAAAAGTTTCTTCACTATTCTTCATCAATTTGATGGAGTCAATGGCTTGCAAATCAAACCAAAAAATTATGATCAATGGCTTGGAGTTCAATTCTCTCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGCATGGAGCAATGGGAGAATTCACTCAGCCACTCATAGAGTTATAATTGAGGGAAACAAAGAGAGATATTCAACTGGCCTATTTTCATACCACAAAGGAATTATTGAAATTCCACAAGAATTGGTGGATGAGAAACATCCCTTGAGATTTAACCCTTTTAACCATTATGGATTACTTGGTTATTTTTCCACTGATGATTGTGACAAAATCCCTTCAACTGCCAATGCTTATTGTGGAGTTTGA

Protein sequence

MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELVDEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV*
Homology
BLAST of CsGy3G023650 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 5.8e-61
Identity = 131/318 (41.19%), Postives = 190/318 (59.75%), Query Frame = 0

Query: 15  KLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCNSSSSPKLNS 74
           +L  +D  D +  P S K W E +  V  ALE+YGCF   +D              KL+ 
Sbjct: 13  QLPVIDFSDQNLKPGSSK-WDEVTADVLKALEDYGCFEASFD--------------KLSV 72

Query: 75  NNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIENASSYEAVQ 134
                +F+ +++LF+LP+ TK +NVS KP+HG+L C       L++SLGI +A+  E V 
Sbjct: 73  ELNRSVFEAMEDLFELPIPTKQRNVSSKPFHGYL-CHN-----LYESLGINDANVLEKVN 132

Query: 135 FFVNLLWPS-GNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIASTTYLLRT 194
            F   LWP  GN    E +  +S+++ E++ +V+RM+ ES+G+  Y D H+ ST YL R 
Sbjct: 133 DFTQQLWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRL 192

Query: 195 MKYRIPKMGEEN------IGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFSPN 254
           MKY  P   +++      +G  +HTDK+  TILHQ+  V+GL++K K+ D+W+ V+ S +
Sbjct: 193 MKYTSPPDDDDDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQD 252

Query: 255 SFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKG--IIEIPQELVDEKHP 314
           S LVM GD+  A  NGR+HS  HRVI+ G K RYSTGLFS  K   II+ P+ELVD++HP
Sbjct: 253 SVLVMVGDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHP 307

Query: 315 LRFNPFNHYGLLGYFSTD 324
             F PF +   L +F T+
Sbjct: 313 RIFKPFEYTDFLHFFQTE 307

BLAST of CsGy3G023650 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.6e-61
Identity = 131/317 (41.32%), Postives = 190/317 (59.94%), Query Frame = 0

Query: 15  KLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCNSSSSPKLNS 74
           +L  +D  D +  P S K W E    V  ALE+YGCF   +D              KL+ 
Sbjct: 13  QLPVIDFSDQNLKPGSSK-WDEVKADVLKALEDYGCFEAFFD--------------KLSV 72

Query: 75  NNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIENASSYEAVQ 134
                +F+ +++LF+LP+ TK +NVS KP+HG+L C       L++SLGI++A+  E V 
Sbjct: 73  ELNRSVFEAMEDLFELPIPTKQRNVSSKPFHGYL-CHN-----LYESLGIDDANVLEKVN 132

Query: 135 FFVNLLWPS-GNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIASTTYLLRT 194
            F   LWP  GN    E +  +S+++ E++ +V+RM+ ES+G+  Y D H+ ST YL R 
Sbjct: 133 DFTQQLWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRL 192

Query: 195 MKYRIPKMGEEN-----IGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFSPNS 254
           MKY  P   +++     +G  +HTDK+  TILHQ+  V+GL++K K+ D+W+ V+ S +S
Sbjct: 193 MKYTSPPDDDDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQDS 252

Query: 255 FLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKG--IIEIPQELVDEKHPL 314
            LVM GD+  A  NGR+HS  HRVI+ G K RYSTGLFS  K   II+ P+ELVD++HP 
Sbjct: 253 VLVMVGDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPR 306

Query: 315 RFNPFNHYGLLGYFSTD 324
            F PF +   L +F T+
Sbjct: 313 IFKPFEYTDFLHFFQTE 306

BLAST of CsGy3G023650 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.3e-60
Identity = 131/316 (41.46%), Postives = 190/316 (60.13%), Query Frame = 0

Query: 15  KLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCNSSSSPKLNS 74
           +L  +D  D +  P S K W E +  V  ALE+YGCF   +D              KL+ 
Sbjct: 6   QLPVIDFSDQNLKPGSSK-WDEVTADVLKALEDYGCFEASFD--------------KLSV 65

Query: 75  NNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIENASSYEAVQ 134
                +F+ +++LF+LP+ TK +NVS K +HG+L C       L++SLGI +A+  E V 
Sbjct: 66  ELNRSVFEAMEDLFELPIPTKQRNVSSKLFHGYL-CHN-----LYESLGINDANVLEKVN 125

Query: 135 FFVNLLWPS-GNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIASTTYLLRT 194
            F   LWP  GN    E +  +S+++ E++ +V+RM+ ES+G+ KY D H+ ST YL R 
Sbjct: 126 DFTQQLWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRL 185

Query: 195 MKYRIPKMGEEN----IGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFSPNSF 254
           MKY  P   +++    +G  +HTDK+  TILHQ+  V+GL++K K+ D+W+ V+ S +S 
Sbjct: 186 MKYTSPPDDDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQDSV 245

Query: 255 LVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKG--IIEIPQELVDEKHPLR 314
           LVM GD+  A  NGR+HS  HRVI+ G K RYSTGLFS  K   II+ P+ELVD++HP  
Sbjct: 246 LVMVGDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRI 298

Query: 315 FNPFNHYGLLGYFSTD 324
           F PF +   L +F T+
Sbjct: 306 FKPFEYTDFLHFFQTE 298

BLAST of CsGy3G023650 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.3e-56
Identity = 124/318 (38.99%), Postives = 180/318 (56.60%), Query Frame = 0

Query: 7   AKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCNS 66
           A L +P+I LS  + K   +       W     QVR+ALEEYGCF    D          
Sbjct: 5   APLRLPVIDLSMKNLKPGTTS------WNSVRTQVREALEEYGCFEAVID---------- 64

Query: 67  SSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIEN 126
           + SP+L     + +     EL +LPLETK+ N +   Y GF       +  L++ +G+  
Sbjct: 65  AVSPELQ----KAVCNKGHELLNLPLETKMLNGNKPEYDGFTS-----IPNLNEGMGVGR 124

Query: 127 ASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIAS 186
            +  E V+ F NL+WP GN  FCE +++Y K+++E++ ++K MVFES+G+ K++DS   S
Sbjct: 125 ITDLEKVERFTNLMWPEGNKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCES 184

Query: 187 TTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFSP 246
           T YLL  M+Y+ P     +     H DKS  TI++Q D V GL+ + K+  +W  +  + 
Sbjct: 185 TNYLLHFMRYQQPGKDGRSPALSLHKDKSILTIVNQND-VKGLEFETKD-GEW--ILPTA 244

Query: 247 NSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELVDEKHPL 306
           ++ +V+ GD  +AWSNGR+HS  HRV +  N+ R ST  FS+ K IIE P ELVDE+HPL
Sbjct: 245 DNHIVLLGDCFMAWSNGRLHSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPL 293

Query: 307 RFNPFNHYGLLGYFSTDD 325
            FNPF    LL Y  T +
Sbjct: 305 LFNPFEITELLAYCFTKE 293

BLAST of CsGy3G023650 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.1e-48
Identity = 135/451 (29.93%), Postives = 198/451 (43.90%), Query Frame = 0

Query: 9   LEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCNSSS 68
           L++P+I L+     D   +P S K W E    VR ALE++GCF   YD            
Sbjct: 6   LQLPLINLA-----DKTLEPGSSK-WAEVRSDVRKALEDFGCFEASYD------------ 65

Query: 69  SPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIENAS 128
             K++    E I K ++ELF LP+ETK +NV  KPY G+L+        L +SLGI NA+
Sbjct: 66  --KVSLELQESIMKTMEELFALPVETKQRNVCPKPYVGYLNHNN-----LSESLGISNAN 125

Query: 129 SYEAVQFFVNLLWP--SGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIAS 188
             E V  F   LWP   GN +  + +  +++K+ E++ +V+RMV ES+G+ KY D H+ S
Sbjct: 126 ILENVNEFTQQLWPDGDGNENISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKS 185

Query: 189 TTYLLRTMKYRIPKMGEEN----------------------------------------- 248
           T Y +R MKY  P  G+ N                                         
Sbjct: 186 TEYRMRLMKYIAPPEGDANTTVDDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVN 245

Query: 249 ------------------------------------------------------------ 308
                                                                       
Sbjct: 246 AGVGANVNADTGVNDNLNVDAEANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGAT 305

Query: 309 ---------------IGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFSPNSFL 339
                          +G   HTDK+ FT+L Q + + GL++K K+ ++W+ V+ SPN+F+
Sbjct: 306 SGDDVEANDDNEEKKLGLPCHTDKNLFTVLFQHE-IEGLEVKTKD-EKWIRVKPSPNTFI 365

BLAST of CsGy3G023650 vs. NCBI nr
Match: XP_011651543.2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8650761.1 hypothetical protein Csa_017427 [Cucumis sativus])

HSP 1 Score: 702 bits (1811), Expect = 2.46e-255
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60
           MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES
Sbjct: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60

Query: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120
           SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ
Sbjct: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120

Query: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180
           SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY
Sbjct: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180

Query: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240
           DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL
Sbjct: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240

Query: 241 GVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELV 300
           GVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELV
Sbjct: 241 GVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELV 300

Query: 301 DEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 338
           DEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV
Sbjct: 301 DEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 338

BLAST of CsGy3G023650 vs. NCBI nr
Match: XP_038878200.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida])

HSP 1 Score: 542 bits (1397), Expect = 1.62e-192
Identity = 268/338 (79.29%), Postives = 293/338 (86.69%), Query Frame = 0

Query: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60
           MGI   AK EVP+IK S   + D+D  P+S K WLE   QVR ALEEYGCFL EY+    
Sbjct: 1   MGIQSSAKSEVPVIKFSI--ENDEDLHPQSTK-WLETCSQVRAALEEYGCFLAEYE---- 60

Query: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120
                 S+S KL    Y+EIFKGLKELFDLPLETK+KNVSDKPYHGFLD   PFVLPLHQ
Sbjct: 61  ------SASSKL----YDEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQ 120

Query: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180
           SLGIENA+S++AV+ F+ LLWPSGNHHFCE LF+YS+ ISEMEQLVK+MVFESYGVGKYY
Sbjct: 121 SLGIENATSFQAVESFLKLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYY 180

Query: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240
           DSHIASTTYLLRTMKYRIP+MGEENIGAEAHTDKSFFTILHQ +GVNGL+IKPKN D WL
Sbjct: 181 DSHIASTTYLLRTMKYRIPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWL 240

Query: 241 GVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELV 300
            +QFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEG+KERYST LFSYHKGIIEIPQELV
Sbjct: 241 RLQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELV 300

Query: 301 DEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 338
           DE+HPLRFNPFNHYGLLGYFSTD+C+K+PSTANAYCGV
Sbjct: 301 DEQHPLRFNPFNHYGLLGYFSTDECEKMPSTANAYCGV 321

BLAST of CsGy3G023650 vs. NCBI nr
Match: XP_016900413.1 (PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucumis melo])

HSP 1 Score: 487 bits (1253), Expect = 7.93e-172
Identity = 230/243 (94.65%), Postives = 234/243 (96.30%), Query Frame = 0

Query: 96  IKNVSDKPYHGFLDCRTPFVLPLHQSLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAY 155
           +KNVSDKPYHGFLD R PFVLPLHQSLGIENASSYEAVQ FVNLLWPSGNHHFCE LFAY
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 156 SKKISEMEQLVKRMVFESYGVGKYYDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 215
           SKKI+EMEQLVKRMVFESYGVGKY DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 216 FFTILHQFDGVNGLQIKPKNYDQWLGVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIE 275
           FFTILHQFDGVNGLQIKPKNYDQWLGV FSPN FLVMAGDACLAWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 276 GNKERYSTGLFSYHKGIIEIPQELVDEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAY 335
           GNKERYST LFSYHKGIIEIPQELVD+KHPLRF PFNHYGLLGYFSTDDCDK+PSTANAY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

Query: 336 CGV 338
           CGV
Sbjct: 241 CGV 243

BLAST of CsGy3G023650 vs. NCBI nr
Match: XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 484 bits (1245), Expect = 2.16e-169
Identity = 240/338 (71.01%), Postives = 274/338 (81.07%), Query Frame = 0

Query: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60
           MG       EVP+I  S  + +     P+S K W+E    VR ALEEYGCFL EY++D  
Sbjct: 1   MGFRSSTNPEVPVISFSIENLR-----PKSTK-WVETCSHVRAALEEYGCFLAEYENDV- 60

Query: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120
                     +L+    + IF GL++LFDLPLETK+KNVSDKPYHGFLD R PFVLPLH+
Sbjct: 61  ------EQEVELD----DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHE 120

Query: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180
           S GIENA+S+E V+ FV L+WPSGNHHFCE LFAY+K +SEME+LVKRMVFESYGV KYY
Sbjct: 121 SFGIENAASFEGVESFVKLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYY 180

Query: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240
           DSHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKSFFTILHQ +GVNGL+IK K  D W+
Sbjct: 181 DSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDHWI 240

Query: 241 GVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELV 300
           G+QF+PNSFLVMAGDACLAWSNGRIHSATHRVIIEG+KERYST LFSYHKG+IEIP ELV
Sbjct: 241 GLQFNPNSFLVMAGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPNELV 300

Query: 301 DEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 338
           DE+ PL+FNPFNHYGLLGYFS +DC+K PSTA AYCGV
Sbjct: 301 DEQFPLKFNPFNHYGLLGYFSKEDCEKTPSTAGAYCGV 320

BLAST of CsGy3G023650 vs. NCBI nr
Match: XP_022153580.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia])

HSP 1 Score: 482 bits (1241), Expect = 7.86e-169
Identity = 244/333 (73.27%), Postives = 276/333 (82.88%), Query Frame = 0

Query: 6   LAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCN 65
           LAK ++P+I LS      ++  PES   WLE    +R ALEEYGCFLGE+          
Sbjct: 7   LAKAKLPVINLSI-----ENVSPESTN-WLEICSHLRAALEEYGCFLGEF---------- 66

Query: 66  SSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIE 125
            S++P+L+    +EIFKGL+ELFDLPLETK+KNVSDKPYHGFLD RTPFVLPLHQS GIE
Sbjct: 67  GSATPELD----DEIFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIE 126

Query: 126 NASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIA 185
           N+ S+EAVQ FVNL+WPSGN  FCE +F+Y+K +SEME+LVK MVFESYGV KYYDSHIA
Sbjct: 127 NSESFEAVQSFVNLMWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIA 186

Query: 186 STTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFS 245
           STTYLLRTMKYRIP+MGEENIGA+AHTDKSFFTILHQ + VNGL+IK K+   WLGV+F+
Sbjct: 187 STTYLLRTMKYRIPEMGEENIGADAHTDKSFFTILHQNE-VNGLEIKTKD-GHWLGVEFT 246

Query: 246 PNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELVDEKHP 305
           PNSFLVMAGDACLAWSNGRIHSATHRV+IEG KERYST LFSYHKGIIEIP ELVDEKHP
Sbjct: 247 PNSFLVMAGDACLAWSNGRIHSATHRVMIEGTKERYSTALFSYHKGIIEIPYELVDEKHP 306

Query: 306 LRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 338
           LRF+PFNHYGLL YFST D  K+PSTA AYCGV
Sbjct: 307 LRFHPFNHYGLLAYFSTHDSHKMPSTAKAYCGV 317

BLAST of CsGy3G023650 vs. ExPASy TrEMBL
Match: A0A0A0LBU1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G555690 PE=4 SV=1)

HSP 1 Score: 530 bits (1364), Expect = 9.16e-189
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0

Query: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60
           MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES
Sbjct: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60

Query: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120
           SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ
Sbjct: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120

Query: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180
           SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY
Sbjct: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180

Query: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240
           DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL
Sbjct: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240

Query: 241 GVQFSPNSFLVMAGDACL 258
           GVQFSPNSFLVMAGDACL
Sbjct: 241 GVQFSPNSFLVMAGDACL 258

BLAST of CsGy3G023650 vs. ExPASy TrEMBL
Match: A0A1S4DWR1 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103490084 PE=3 SV=1)

HSP 1 Score: 487 bits (1253), Expect = 3.84e-172
Identity = 230/243 (94.65%), Postives = 234/243 (96.30%), Query Frame = 0

Query: 96  IKNVSDKPYHGFLDCRTPFVLPLHQSLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAY 155
           +KNVSDKPYHGFLD R PFVLPLHQSLGIENASSYEAVQ FVNLLWPSGNHHFCE LFAY
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 156 SKKISEMEQLVKRMVFESYGVGKYYDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 215
           SKKI+EMEQLVKRMVFESYGVGKY DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 216 FFTILHQFDGVNGLQIKPKNYDQWLGVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIE 275
           FFTILHQFDGVNGLQIKPKNYDQWLGV FSPN FLVMAGDACLAWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 276 GNKERYSTGLFSYHKGIIEIPQELVDEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAY 335
           GNKERYST LFSYHKGIIEIPQELVD+KHPLRF PFNHYGLLGYFSTDDCDK+PSTANAY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

Query: 336 CGV 338
           CGV
Sbjct: 241 CGV 243

BLAST of CsGy3G023650 vs. ExPASy TrEMBL
Match: A0A6J1DH70 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN=LOC111021051 PE=3 SV=1)

HSP 1 Score: 482 bits (1241), Expect = 3.81e-169
Identity = 244/333 (73.27%), Postives = 276/333 (82.88%), Query Frame = 0

Query: 6   LAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCN 65
           LAK ++P+I LS      ++  PES   WLE    +R ALEEYGCFLGE+          
Sbjct: 7   LAKAKLPVINLSI-----ENVSPESTN-WLEICSHLRAALEEYGCFLGEF---------- 66

Query: 66  SSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIE 125
            S++P+L+    +EIFKGL+ELFDLPLETK+KNVSDKPYHGFLD RTPFVLPLHQS GIE
Sbjct: 67  GSATPELD----DEIFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIE 126

Query: 126 NASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIA 185
           N+ S+EAVQ FVNL+WPSGN  FCE +F+Y+K +SEME+LVK MVFESYGV KYYDSHIA
Sbjct: 127 NSESFEAVQSFVNLMWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIA 186

Query: 186 STTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFS 245
           STTYLLRTMKYRIP+MGEENIGA+AHTDKSFFTILHQ + VNGL+IK K+   WLGV+F+
Sbjct: 187 STTYLLRTMKYRIPEMGEENIGADAHTDKSFFTILHQNE-VNGLEIKTKD-GHWLGVEFT 246

Query: 246 PNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELVDEKHP 305
           PNSFLVMAGDACLAWSNGRIHSATHRV+IEG KERYST LFSYHKGIIEIP ELVDEKHP
Sbjct: 247 PNSFLVMAGDACLAWSNGRIHSATHRVMIEGTKERYSTALFSYHKGIIEIPYELVDEKHP 306

Query: 306 LRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 338
           LRF+PFNHYGLL YFST D  K+PSTA AYCGV
Sbjct: 307 LRFHPFNHYGLLAYFSTHDSHKMPSTAKAYCGV 317

BLAST of CsGy3G023650 vs. ExPASy TrEMBL
Match: A0A6J1GA17 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111452291 PE=3 SV=1)

HSP 1 Score: 479 bits (1232), Expect = 9.94e-168
Identity = 237/338 (70.12%), Postives = 274/338 (81.07%), Query Frame = 0

Query: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60
           MG     K EVP+I  S      ++  P+S K W+E    VR ALEEYGCFL EY++D  
Sbjct: 1   MGFRSSTKPEVPVINFSI-----ENLGPKSTK-WVETCTHVRAALEEYGCFLAEYENDV- 60

Query: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120
                     +L+    + IF GL++LFDLPL+TK+KNVSDKPYHGFLD R PFVLPLH+
Sbjct: 61  ------EQEVELD----DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHE 120

Query: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180
           S GIENA+S+E V+ FV L+WPSGNHHFC+ LFAY+K +SEME+LVKRMVFESYGV KYY
Sbjct: 121 SFGIENAASFEGVESFVKLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYY 180

Query: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240
           DSHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKSFFTILHQ +GVNGL+IK K  D W+
Sbjct: 181 DSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDHWI 240

Query: 241 GVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELV 300
           G+QF+ NSFLVMAGDACLAWSNGRIHSATHRVIIEG+KERYST LFSYHKG+IEIP ELV
Sbjct: 241 GLQFNSNSFLVMAGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELV 300

Query: 301 DEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 338
           DE+ PL+FNPFNHYGLLGYFS ++C+K PSTA AYCGV
Sbjct: 301 DEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV 320

BLAST of CsGy3G023650 vs. ExPASy TrEMBL
Match: A0A6J1K6A3 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LOC111492707 PE=3 SV=1)

HSP 1 Score: 476 bits (1226), Expect = 7.04e-167
Identity = 239/341 (70.09%), Postives = 270/341 (79.18%), Query Frame = 0

Query: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60
           MG     K EVP I  S  + +     P+S K W+E    VR ALEEYGCFL E +    
Sbjct: 1   MGFRSSKKPEVPAINFSIENLR-----PKSTK-WVETCSHVRAALEEYGCFLAECE---- 60

Query: 61  SCSCNSSSSPKLNSNNYE---EIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLP 120
                         NN E    IF GL++LFDLPLETK+KNVSDKPYHGFLD R PFVLP
Sbjct: 61  --------------NNVELDDAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLP 120

Query: 121 LHQSLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVG 180
           LH+S GIENA+S+E V+ FV L+WPSGNHHFCE LFAY+K +SEME+LVKRMVFESYGV 
Sbjct: 121 LHESFGIENAASFEGVESFVKLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVE 180

Query: 181 KYYDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYD 240
           KYYDSHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKSFFTILHQ +GVNGL+IK K  D
Sbjct: 181 KYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDD 240

Query: 241 QWLGVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQ 300
            W+G+QF+PNSFLVMAGDACLAWSNGRIHSATHRV+IEG+KERYST LFSYHKG+IEIP 
Sbjct: 241 HWIGLQFNPNSFLVMAGDACLAWSNGRIHSATHRVLIEGSKERYSTALFSYHKGVIEIPD 300

Query: 301 ELVDEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 338
           ELVDE+ PL+FNPFNHYGLLGYFS ++C+K PSTA AYCGV
Sbjct: 301 ELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV 316

BLAST of CsGy3G023650 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 290.0 bits (741), Expect = 2.4e-78
Identity = 140/333 (42.04%), Postives = 209/333 (62.76%), Query Frame = 0

Query: 6   LAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCN 65
           L   +VPI+ L++  +   ++       W   S++  +ALEEYGCFL  YD         
Sbjct: 5   LKTTKVPILDLTSQQELKPNT-----STWRSVSREACEALEEYGCFLAVYDG-------- 64

Query: 66  SSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIE 125
                 +     + IF   +ELFDLP ETK KNV++KPYHG++  + P V+PLH+ LG++
Sbjct: 65  ------VTQQLDDSIFAAAEELFDLPTETKKKNVNEKPYHGYVG-QMP-VIPLHEGLGVD 124

Query: 126 NASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIA 185
             ++ E  Q F +L+WP GN  FC  +  +S  ++E+++LV RM+FE+YGV K+Y+SH+ 
Sbjct: 125 YVTNKEIAQRFTHLMWPQGNDRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVG 184

Query: 186 STTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFS 245
           S TYLL+ +KY  P           HTDK+F +ILHQ D VNGL++K K+  +W+ +Q  
Sbjct: 185 SKTYLLKFLKYLAPPESISMPAFPQHTDKTFLSILHQND-VNGLEVKSKD-GEWISLQLP 244

Query: 246 PNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELVDEKHP 305
           P S++VMAGD  + WSN RI S  HRV +EG+K RY+ GLFS+   ++ IP+ELVD+KHP
Sbjct: 245 PKSYVVMAGDISMGWSNDRIRSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHP 304

Query: 306 LRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 339
           L + PF++  L+ +++T +  +  ST  AYCG+
Sbjct: 305 LMYKPFDNIALINFYTTKEGREANSTLKAYCGI 314

BLAST of CsGy3G023650 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-77
Identity = 142/323 (43.96%), Postives = 204/323 (63.16%), Query Frame = 0

Query: 15  KLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCNSSSSPKLNS 74
           K+  +D   +D  P   K W    + +R ALEEYGCF+ +  D             K   
Sbjct: 4   KIPTLDFSREDLKP-GTKYWESTRENIRQALEEYGCFIIDLKD-------------KTPL 63

Query: 75  NNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIENASSYEAVQ 134
           +  + +F  L +LFDLP +TK+KN  DKP +G++  + P  LPLH+SLGI+NA+S EA +
Sbjct: 64  DLLDRVFGSLVDLFDLPTQTKMKNKYDKPLNGYVG-QIP-ALPLHESLGIDNATSLEATR 123

Query: 135 FFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIASTTYLLRTM 194
            F  L+WP GN HF E L+ Y++  +E++Q+V RMVF+SY V KYYD +I STTYLLR +
Sbjct: 124 SFTGLMWPQGNEHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVL 183

Query: 195 KYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFSPNSFLVMAG 254
           K R P      +G   HTDKSF TILHQ D VNGL+++ +  ++      SP+ F+V+AG
Sbjct: 184 KNRAPNNENPTLGFVTHTDKSFTTILHQ-DQVNGLEMETREGERININLSSPSLFMVVAG 243

Query: 255 DACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELVDEKHPLRFNPFNHY 314
           DA +AWSN R+ S  H+V++ G  +RYS G+F+++ G +++P+EL+D +HPL + PF+H 
Sbjct: 244 DALMAWSNDRVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHL 303

Query: 315 GLLGYFSTDDCDKIPSTANAYCG 338
           GLL ++ TD   K      AYCG
Sbjct: 304 GLLRFYRTDTGYKSECPIKAYCG 309

BLAST of CsGy3G023650 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 253.8 bits (647), Expect = 1.9e-67
Identity = 135/335 (40.30%), Postives = 199/335 (59.40%), Query Frame = 0

Query: 6   LAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCN 65
           L  L +P+I  SN + K    +PE    W      V+ AL++YG F   +D         
Sbjct: 7   LLPLRLPVIDFSNKNLK--PGEPE----WDLTRADVQKALQDYGYFEASFD--------- 66

Query: 66  SSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIE 125
                ++     + +F  L+ELFDLPL+TK++NVS KP+HG++  + P V PL++S+GI+
Sbjct: 67  -----RIPFELRKSVFGALEELFDLPLQTKLRNVSKKPFHGYVG-QYPMV-PLYESMGID 126

Query: 126 NASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIA 185
           ++   E V  F   LWP GN  F   + ++SKK+SE++  ++RM+ ES+G+ KY D H+ 
Sbjct: 127 DSDIAEKVDAFTEKLWPQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLH 186

Query: 186 STTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFS 245
           ST YLLR MKY+ P   E  +G  AHTDK+  TIL+Q + V GL+++ K+   W+ V+ +
Sbjct: 187 STNYLLRVMKYKGPDTEETKVGLNAHTDKNIVTILYQ-NHVEGLEVQTKD-KNWIKVKPT 246

Query: 246 PNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKG--IIEIPQELVDEK 305
            +SF VM GD+  A  NGR+HS  HRV++ G + RYS GLFS  K   I+  P ELVDE+
Sbjct: 247 QDSFTVMIGDSLYALLNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEE 306

Query: 306 HPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 339
           HP  F PF+H   L ++ T+   +  S    YCG+
Sbjct: 307 HPRLFKPFDHVEFLQFYYTEAGQRSQSALKTYCGI 317

BLAST of CsGy3G023650 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 250.0 bits (637), Expect = 2.8e-66
Identity = 135/334 (40.42%), Postives = 196/334 (58.68%), Query Frame = 0

Query: 10  EVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCNSSSS 69
           E+PII LS+ + K      ++ +LW      VR+A+E +G F+ EY++            
Sbjct: 8   ELPIINLSDKNLK------QNTELWSFTRDSVREAMEHHGWFVAEYNN------------ 67

Query: 70  PKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIENASS 129
               +  ++ I +  KEL DLP+E K+KN + K  HG++   +    P+H+ LGI+  + 
Sbjct: 68  --FPTGLHQSILEAAKELLDLPVEIKLKNENHKAGHGYITMMSD-GQPVHEGLGIDQVND 127

Query: 130 YEAVQFFVNLLWP---SGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIAS 189
            +  + F  L+WP     N  FCE + AY+K  +E+EQLV RM+FESY V KY + +I  
Sbjct: 128 VQQCRGFSRLMWPDDHDDNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGG 187

Query: 190 TTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFSP 249
           T YLLR +KYR    GE N    +HTDKSF +ILHQ + + GL +K +  D W     SP
Sbjct: 188 TRYLLRLLKYRRLPNGEPNRKFISHTDKSFISILHQ-NHITGLMLKSEKEDVWYHFTPSP 247

Query: 250 NSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELVDEKHPL 309
             F+V+AGDA +AWSN RI +  H+V +E  + RYS G FS+ +G+I  P+E+VD+ HPL
Sbjct: 248 TRFVVIAGDAIMAWSNDRIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPL 307

Query: 310 RFNPFNHYGLLGYFSTDDCD-KIPST-ANAYCGV 339
            +NPF+H GLL Y+ T +   K   T   AYCG+
Sbjct: 308 AYNPFHHDGLLEYYETLEVHLKAHRTMTKAYCGI 319

BLAST of CsGy3G023650 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 236.1 bits (601), Expect = 4.1e-62
Identity = 131/318 (41.19%), Postives = 190/318 (59.75%), Query Frame = 0

Query: 15  KLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDESSCSCNSSSSPKLNS 74
           +L  +D  D +  P S K W E +  V  ALE+YGCF   +D              KL+ 
Sbjct: 13  QLPVIDFSDQNLKPGSSK-WDEVTADVLKALEDYGCFEASFD--------------KLSV 72

Query: 75  NNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQSLGIENASSYEAVQ 134
                +F+ +++LF+LP+ TK +NVS KP+HG+L C       L++SLGI +A+  E V 
Sbjct: 73  ELNRSVFEAMEDLFELPIPTKQRNVSSKPFHGYL-CHN-----LYESLGINDANVLEKVN 132

Query: 135 FFVNLLWPS-GNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYYDSHIASTTYLLRT 194
            F   LWP  GN    E +  +S+++ E++ +V+RM+ ES+G+  Y D H+ ST YL R 
Sbjct: 133 DFTQQLWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRL 192

Query: 195 MKYRIPKMGEEN------IGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVQFSPN 254
           MKY  P   +++      +G  +HTDK+  TILHQ+  V+GL++K K+ D+W+ V+ S +
Sbjct: 193 MKYTSPPDDDDDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQD 252

Query: 255 SFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKG--IIEIPQELVDEKHP 314
           S LVM GD+  A  NGR+HS  HRVI+ G K RYSTGLFS  K   II+ P+ELVD++HP
Sbjct: 253 SVLVMVGDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHP 307

Query: 315 LRFNPFNHYGLLGYFSTD 324
             F PF +   L +F T+
Sbjct: 313 RIFKPFEYTDFLHFFQTE 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZTA35.8e-6141.19Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q945B67.6e-6141.32Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q944Z51.3e-6041.46Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ363.3e-5638.99Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q945B51.1e-4829.932-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
XP_011651543.22.46e-255100.00probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8650761... [more]
XP_038878200.11.62e-19279.292-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida][more]
XP_016900413.17.93e-17294.65PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucumis melo][more]
XP_023525673.12.16e-16971.012-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo][more]
XP_022153580.17.86e-16973.272-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A0A0LBU19.16e-189100.00Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A1S4DWR13.84e-17294.652-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1DH703.81e-16973.272-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1GA179.94e-16870.12probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1K6A37.04e-16770.092-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G52800.12.4e-7842.042-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52790.12.0e-7743.962-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.11.9e-6740.302-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G15540.12.8e-6640.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G03070.14.1e-6241.192-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 201..285
e-value: 4.6E-16
score: 59.2
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 4..320
e-value: 1.6E-48
score: 167.6
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 12..337
NoneNo IPR availablePANTHERPTHR47990:SF172-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKEcoord: 12..337
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 9..319
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 186..290
score: 9.446114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G023650.1CsGy3G023650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding